Comprehensive Tool for Differential Expression Analysis in MS Proteomics
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Annotation File

TMT

Ensure empty channels/ TMT labels are set as "none" under the column Label.

Example for an IP-MS TMT10 run of 3 baits and 1 control with two repeats per condition.

Sample file (IP-MS)

Example for an IP-MS TMT10 run for cancer driver fusion FUS-ERG and 1 control with two repeats per condition.

Sample file (Fusion IP-MS)

Example for benchmarking dataset (downsampled) of a TMT9 run of human yeast proteome with a yeast proteome spike-in at specific ratios. Run on Orbitrap Fusion Lumos.

Sample file (Whole-proteome)

Example for phospho-proteome benchmarking dataset (downsampled) of a TMT10 run of human with a yeast proteome spike-in at specific ratios. Published by Hogrebe et al. Nature Communications 2018 as TMT MS3 OTMC.

Sample file (Phospho-proteome)


SILAC

Example for an IP-MS (heavy and light - two channels) run on METTL3 with a forward and reverse repeat.

Sample file (IP-MS - Protein level)


DIA-LFQ

Example for an IP-MS run on Bruker of 4 conditions with 3 repeats each.

Sample file (IP-MS)

Example for benchmarking dataset (downsampled) of human yeast proteome with a yeast proteome spike-in at specific ratios. Run on Bruker timsTOF with 80 min gradient.

Sample file (Whole-proteome)


File used for quantification

TMT

IP-MS TMT10 run (downsampled) of 3 baits and 1 control with two repeats per condition.

Sample file (IP-MS) S/N file for Proteome Discoverer search
Sample file (IP-MS) Raw reporter intensity file for Proteome Discoverer search

IP-MS TMT10 run for cancer driver fusion FUS-ERG and 1 control with two repeats per condition.

Sample file (Fusion IP-MS) S/N file for Proteome Discoverer search
Sample file (Fusion IP-MS) Raw reporter intensity file for Proteome Discoverer search

Benchmarking dataset (downsampled) of a TMT9 run of human yeast proteome with a yeast proteome spike-in at specific ratios. Run on Orbitrap Fusion Lumos.

Sample file (whole proteome) S/N file for Proteome Discoverer search
Sample file (whole proteome) Raw reporter intensity file for Proteome Discoverer search
Sample file (whole proteome) Raw reporter intensity file for COMET search

Benchmarking phosphoproteome dataset (downsampled) of a TMT9 run of human with a yeast proteome spike-in at specific ratios. Published by Hogrebe et al. Nature Communications 2018.

Sample file (phospho proteome) S/N file for Proteome Discoverer search
Sample file (phospho proteome) Raw reporter intensity file for Proteome Discoverer search
Sample file (phospho proteome) Raw reporter intensity file for COMET search

SILAC

IP-MS run on METTL3 (light and heavy) Wild Type -vs- Control Forward run.

Sample file (IP-MS) COMET search

IP-MS run on METTL3 (light and heavy) Wild Type -vs- Control Reverse run.

Sample file (IP-MS) COMET search


DIA-LFQ

IP-MS run on Bruker of 4 conditions with 3 repeats each.

Sample file (IP-MS - Protein level)
Sample file (IP-MS - Full Information for # precursor assignment)

Benchmarking dataset (downsampled) of human yeast proteome with a yeast proteome spike-in at specific ratios. Run on Bruker timsTOF with 80 min gradient.

Sample file (Whole Proteome - Protein level)
Sample file (Whole Proteome - Precursor level)
Sample file (Whole Proteome - Full Information for # precursor assignment)