Checked Confidence Identifying Node PSM Ambiguity Sequence Annotated Sequence Modifications # Proteins Master Protein Accessions Protein Accessions # Missed Cleavages Charge DeltaScore DeltaCn Rank Search Engine Rank m/z [Da] MH+ [Da] Theo. MH+ [Da] DeltaM [ppm] Deltam/z [Da] Intensity Activation Type MS Order Isolation Interference [%] SPS Mass Matches [%] Average Reporter S/N Ion Inject Time [ms] RT [min] First Scan Spectrum File File ID Abundance: 126 Abundance: 127N Abundance: 127C Abundance: 128N Abundance: 128C Abundance: 129N Abundance: 129C Abundance: 130N Abundance: 130C Abundance: 131 Quan Info XCorr # Protein Groups Percolator q-Value Percolator PEP False High Sequest HT (A2) Unambiguous GRAEGEWEDQEALDYFSDK [K].gRAEGEWEDQEALDYFsDk.[E] N-Term(TMT6plex); S17(Phospho); K19(TMT6plex) 3 Q9NYF8 Q9NYF8-3; Q9NYF8; Q9NYF8-2 1 3 0.1318 0 1 1 928.42222 2783.2521 2783.26019 -2.91 -0.0027 3734313.5 CID (Collision Induced Dissociation) MS2 4.839325 40 75.2 22.756 102.792 33757 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 73.6 64.1 61.0 86.9 70.4 71.6 89.9 78.7 79.6 75.9 Excluded by Method 6.45 1 0.0003003 1.3e-05 False High Sequest HT (A2) Unambiguous LENESLPNLSG [K].lENESLPNLsG.[-] N-Term(TMT6plex); S10(Phospho) 1 Q05024 Q05024 0 2 0.437 0 1 1 741.3585 1481.70973 1481.70862 0.75 0.00055 1049494.75 CID (Collision Induced Dissociation) MS2 22.04548 50 143.4 60.0 102.9082 33803 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 41.9 119.3 238.1 41.1 115.6 286.1 56.2 131.7 268.3 136.2 Excluded by Method 2.38 1 0.0003003 0.0002556 False High Sequest HT (A2) Unambiguous GSLEEIIAEGQK [K].gSLEEIIAEGQk.[K] N-Term(TMT6plex); K12(TMT6plex) 1 P02400 P02400 0 3 0.5211 0 1 1 578.00106 1731.98862 1731.98929 -0.39 -0.00022 618819.0 CID (Collision Induced Dissociation) MS2 0.0 80 66.6 60.0 103.7259 34110 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 25.3 60.6 89.3 25.3 55.0 118.6 31.8 62.9 126.1 71.4 3.55 1 0.0003003 4.603e-05 False High Sequest HT (A2) Unambiguous DVPPLSETEATPVPIK [K].dVPPLSETEAtPVPIk.[D] N-Term(TMT6plex); T11(Phospho); K16(TMT6plex) 2 P27816 P27816; P27816-6 0 3 0.3436 0 1 1 744.40428 2231.19827 2231.19764 0.29 0.00021 512941.8125 CID (Collision Induced Dissociation) MS2 0.0 30 70.3 60.0 103.6948 34098 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 71.8 63.7 62.6 68.1 71.6 69.6 81.0 75.2 76.5 63.3 Excluded by Method 4.86 1 0.0003003 1.056e-06 False High Sequest HT (A2) Unambiguous AFGPGLQGGSAGSPARFTIDTK [K].aFGPGLQGGSAGsPARFTIDTk.[G] N-Term(TMT6plex); S13(Phospho); K22(TMT6plex) 2 P21333 P21333; P21333-2 1 3 0.084 0 1 1 891.79824 2673.38018 2673.38019 0.0 0.0 4696624.5 CID (Collision Induced Dissociation) MS2 0.0 20 63.2 26.795 103.5978 34062 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 69.2 63.8 57.0 61.6 56.4 66.5 68.5 69.8 58.7 60.9 Excluded by Method 5.36 1 0.0003003 3.79e-05 False High Sequest HT (A2) Unambiguous GGITTGDISEEETVDNSINTEEYDK [K].gGITTGDIsEEETVDNSINTEEYDk.[L] N-Term(TMT6plex); S9(Phospho); K25(TMT6plex) 1 Q06704 Q06704 0 3 0.0516 0 1 1 1085.50074 3254.48767 3254.48774 -0.02 -2e-05 1127996.5 CID (Collision Induced Dissociation) MS2 0.0 50 46.3 60.0 103.2973 33951 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 10.9 35.8 69.0 11.4 36.8 91.7 17.5 42.8 95.9 51.0 Excluded by Method 8.34 1 0.0003003 4.579e-10 False High Sequest HT (A2) Unambiguous DNEELQEIDNVSLDEPK [K].dNEELQEIDNVsLDEPk.[I] N-Term(TMT6plex); S12(Phospho); K17(TMT6plex) 1 P25037 P25037 0 4 0.4519 0 1 1 632.05695 2525.20598 2525.20603 -0.02 -1e-05 1205698.0 CID (Collision Induced Dissociation) MS2 19.52778 40 110.9 51.481 103.2159 33922 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 33.0 88.0 145.8 44.9 94.4 195.2 44.6 110.7 229.5 122.8 Excluded by Method 6.24 1 0.0003003 5.359e-06 False High Sequest HT (A2) Unambiguous TQSSESRLYELSAEDLEK [K].tQsSESRLYELSAEDLEk.[E] N-Term(TMT6plex); S3(Phospho); K18(TMT6plex) 1 P53885 P53885 1 3 0.0355 0 1 1 875.10173 2623.29064 2623.29043 0.08 7e-05 692120.5 CID (Collision Induced Dissociation) MS2 41.42597 50 32.2 60.0 103.1366 33890 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 12.7 24.9 44.1 16.6 28.0 51.8 19.2 30.3 64.7 29.9 Excluded by Method 5.63 1 0.0003003 2.101e-05 False High Sequest HT (A2) Unambiguous GFSVVADTPELQRIK [K].gFSVVADtPELQRIk.[K] N-Term(TMT6plex); T8(Phospho); K15(TMT6plex) 3 Q14847 Q14847-2; Q14847-3; Q14847 1 3 0.2827 0 1 1 733.40466 2198.19942 2198.19864 0.35 0.00026 1600745.125 CID (Collision Induced Dissociation) MS2 6.219447 30 184.0 60.0 103.1047 33877 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 190.8 172.0 162.6 178.7 172.1 183.6 197.3 203.9 186.4 193.0 Excluded by Method 2.37 1 0.0003003 4.095e-05 False High Sequest HT (A2) Unambiguous CTGGGIILTASHNPGGPENDLGIK [K].cTGGGIILTAsHNPGGPENDLGIk.[Y] N-Term(TMT6plex); C1(Carbamidomethyl); S11(Phospho); K24(TMT6plex) 1 P33401 P33401 0 3 0.0949 0 1 1 972.82841 2916.47067 2916.46908 0.55 0.00053 852780.8125 CID (Collision Induced Dissociation) MS2 0.0 30 33.8 60.0 101.4746 33259 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 13.9 27.3 42.5 14.2 31.6 66.6 15.3 29.6 62.0 35.4 Excluded by Method 4.95 1 0.0003003 0.0001902 False High Sequest HT (A2) Unambiguous IEEVLSPEGSPSK [K].iEEVLsPEGsPSk.[S] N-Term(TMT6plex); S6(Phospho); S10(Phospho); K13(TMT6plex) 2 Q9UEY8 Q9UEY8-2; Q9UEY8 0 3 0.5768 0 1 1 663.99105 1989.95861 1989.95873 -0.06 -4e-05 583311.25 CID (Collision Induced Dissociation) MS2 17.99117 60 161.6 52.159 101.5051 33270 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 164.2 161.7 133.4 178.1 152.2 148.3 193.3 168.4 161.1 155.3 Excluded by Method 4.56 1 0.0003003 4.411e-05 False High Sequest HT (A2) Unambiguous VQGEAVSNIQENTQTPTVQEESEEEEVDETGVEVK [K].vQGEAVSNIQENTQTPTVQEEsEEEEVDETGVEVk.[D] N-Term(TMT6plex); S22(Phospho); K35(TMT6plex) 3 Q13765 Q13765; E9PAV3; E9PAV3-2 0 4 0.0284 0 1 1 1100.52038 4399.05967 4399.06016 -0.11 -0.00012 1810177.5 CID (Collision Induced Dissociation) MS2 3.6309 50 66.5 60.0 100.9468 33061 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 63.8 71.5 49.1 70.2 71.1 63.5 83.7 69.3 56.0 66.4 Excluded by Method 6.7 1 0.0003003 4.643e-06 False High Sequest HT (A2) Unambiguous VQISPDSGGLPERSVSLTGAPESVQK [K].vQISPDsGGLPERSVSLTGAPESVQk.[A] N-Term(TMT6plex); S7(Phospho); K26(TMT6plex) 1 Q92945 Q92945 1 4 0.0028 0 1 1 794.92112 3176.66263 3176.66044 0.69 0.00055 1160165.375 CID (Collision Induced Dissociation) MS2 7.339591 50 56.9 42.396 100.8777 33035 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 53.1 54.2 53.9 58.4 55.9 61.5 59.1 66.6 49.6 56.6 Excluded by Method 3.52 1 0.0003003 0.0001553 False High Sequest HT (A2) Unambiguous DIDSEDEQDPFESHFNQVPEK [K].dIDsEDEQDPFESHFNQVPEk.[F] N-Term(TMT6plex); S4(Phospho); K21(TMT6plex) 1 P40498 P40498 0 3 0.3711 0 1 1 1015.12651 3043.36497 3043.36103 1.3 0.00131 1126846.75 CID (Collision Induced Dissociation) MS2 6.494675 30 63.4 60.0 100.8398 33022 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 13.7 52.0 91.7 19.6 52.4 125.1 23.6 51.5 132.3 72.4 Excluded by Method 5.39 1 0.0003003 0.0001452 False High Sequest HT (A2) Unambiguous SPASDTYIVFGEAK [K].sPASDTYIVFGEAk.[I] N-Term(TMT6plex); K14(TMT6plex) 3 Q13765 Q13765; E9PAV3; E9PAV3-2 0 3 0.3956 0 1 1 648.35564 1943.05236 1943.05261 -0.13 -8e-05 361017.28125 CID (Collision Induced Dissociation) MS2 0.0 50 32.4 60.0 101.0366 33095 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 30.0 30.1 24.3 33.3 34.8 34.1 37.8 31.0 34.2 34.8 Excluded by Method 2.73 1 0.0003003 9.763e-05 False High Sequest HT (A2) Unambiguous VQGEAVSNIQENTQTPTVQEESEEEEVDETGVEVK [K].vQGEAVSNIQENTQTPTVQEEsEEEEVDETGVEVk.[D] N-Term(TMT6plex); S22(Phospho); K35(TMT6plex) 3 Q13765 Q13765; E9PAV3; E9PAV3-2 0 4 0.0678 0 1 1 1100.52135 4399.06358 4399.06016 0.78 0.00085 804390.875 CID (Collision Induced Dissociation) MS2 0.0 30 46.0 45.393 102.3338 33582 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 53.4 46.4 35.6 44.0 40.8 43.5 47.9 48.7 44.3 55.8 Excluded by Method 5.75 1 0.0003003 2.088e-06 False High Sequest HT (A2) Unambiguous NPDEDEFLINSDDEM [K].nPDEDEFLINsDDEm.[-] N-Term(TMT6plex); S11(Phospho); M15(Oxidation) 1 Q08412 Q08412 0 3 0.5033 0 1 1 703.28043 2107.82674 2107.82524 0.71 0.0005 736993.5 CID (Collision Induced Dissociation) MS2 5.929857 60 66.9 60.0 101.5757 33295 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 17.5 52.8 102.4 24.6 52.4 127.4 19.5 63.4 138.1 70.5 Excluded by Method 4.49 1 0.0003003 2.603e-05 False High Sequest HT (A2) Unambiguous SEADPEGLSNSNIVNDADNINSISK [K].sEADPEGLSNSNIVNDADNINSIsk.[T] N-Term(TMT6plex); S24(Phospho); K25(TMT6plex) 1 Q06685 Q06685 0 3 0.0627 0 1 1 1047.84003 3141.50553 3141.49892 2.1 0.0022 496114.71875 CID (Collision Induced Dissociation) MS2 14.46025 70 43.8 60.0 101.7127 33346 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 10.8 30.4 61.2 10.8 32.9 89.5 17.3 43.2 91.5 49.9 8.13 1 0.0003003 4.035e-10 False High Sequest HT (A2) Unambiguous DFSEDLNSFDK [K].dFSEDLNsFDk.[Q] N-Term(TMT6plex); S8(Phospho); K11(TMT6plex) 1 Q04773 Q04773 0 3 0.4485 0 1 1 618.95693 1854.85625 1854.8563 -0.03 -2e-05 724918.25 CID (Collision Induced Dissociation) MS2 0.0 50 137.1 60.0 102.1985 33530 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 44.0 115.3 201.0 47.9 110.2 254.8 53.9 135.3 271.9 136.2 Excluded by Method 4.95 1 0.0003003 7.218e-06 False High Sequest HT (A2) Unambiguous RQSSTPSAPELGQQPDVNISEWK [K].rQSStPSAPELGQQPDVNISEWk.[D] N-Term(TMT6plex); T5(Phospho); K23(TMT6plex) 4 Q93100 Q93100-2; Q93100; Q93100-3; Q93100-4 1 4 0.0019 0 1 1 773.89313 3092.55068 3092.54541 1.7 0.00132 1270201.0 CID (Collision Induced Dissociation) MS2 2.160956 30 52.4 60.0 103.7892 34133 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 51.8 53.8 45.0 46.2 48.5 58.4 63.1 55.6 60.3 41.5 Excluded by Method 5.33 1 0.0003003 6.387e-05 False High Sequest HT (A2) Unambiguous ALGVISNFQSSPK [K].aLGVISNFQSsPk.[Y] N-Term(TMT6plex); S11(Phospho); K13(TMT6plex) 2 O94876 O94876-2; O94876 0 3 0.0322 0 1 1 629.34419 1886.01801 1886.01889 -0.46 -0.00029 1332489.75 CID (Collision Induced Dissociation) MS2 2.130759 40 125.6 60.0 106.4117 35109 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 127.1 123.0 100.5 138.4 122.9 116.9 134.3 138.9 137.8 116.6 Excluded by Method 4.35 1 0.0003003 7.137e-05 False High Sequest HT (A2) Unambiguous LFESDEEETNGENIQINK [K].lFEsDEEETNGENIQINk.[G] N-Term(TMT6plex); S4(Phospho); K18(TMT6plex) 1 Q04500 Q04500 0 3 0.1309 0 1 1 883.08927 2647.25326 2647.25404 -0.3 -0.00026 2200421.75 CID (Collision Induced Dissociation) MS2 21.80801 40 81.7 35.972 106.6199 35186 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 20.4 69.7 134.9 20.6 73.7 145.7 24.6 70.2 173.5 84.0 Excluded by Method 4.89 1 0.0003003 2.485e-06 False High Sequest HT (A2) Unambiguous IENQQGVNNFDEILK [K].iENQQGVNNFDEILk.[V] N-Term(TMT6plex); K15(TMT6plex) 1 P00549 P00549 0 3 0.509 0 1 1 740.40721 2219.20708 2219.20722 -0.06 -5e-05 898358.125 CID (Collision Induced Dissociation) MS2 32.87698 60 152.2 60.0 108.0218 35714 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 40.9 129.6 242.3 45.8 124.4 281.8 53.4 141.8 302.2 159.4 Excluded by Method 4.99 1 0.0003003 2.578e-08 False High Sequest HT (A2) Unambiguous TGPFEVGVHVLPK [K].tGPFEVGVHVLPk.[I] N-Term(TMT6plex); T1(Phospho); K13(TMT6plex) 1 P39954 P39954 0 3 0.6026 0 1 1 640.02466 1918.05941 1918.06036 -0.49 -0.00031 363436.46875 CID (Collision Induced Dissociation) MS2 0.0 40 89.9 60.0 107.6158 35564 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 28.8 74.7 143.6 39.1 76.6 157.7 37.4 76.9 164.7 99.4 Excluded by Method 4.68 1 0.0003003 0.0001939 False High Sequest HT (A2) Unambiguous YSDVEVPASVTGYSFASDGDSGTCSPLRHSFQK [K].ySDVEVPASVTGYSFASDGDSGTcSPLRHsFQk.[Q] N-Term(TMT6plex); C24(Carbamidomethyl); S30(Phospho); K33(TMT6plex) 6 P35611 P35611-2; P35611-6; P35611-4; P35611-3; P35611-5; P35611 1 4 0.0528 0 1 1 1023.22873 4089.89309 4089.89416 -0.26 -0.00027 441450.46875 CID (Collision Induced Dissociation) MS2 9.449142 80 32.0 60.0 107.5583 35542 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 29.1 31.1 25.7 27.0 29.0 29.4 38.7 37.9 37.5 34.7 7.39 1 0.0003003 3.212e-06 False High Sequest HT (A2) Unambiguous VQGEAVSNIQENTQTPTVQEESEEEEVDETGVEVK [K].vQGEAVSNIQENTQTPTVQEEsEEEEVDETGVEVk.[D] N-Term(TMT6plex); S22(Phospho); K35(TMT6plex) 3 Q13765 Q13765; E9PAV3; E9PAV3-2 0 4 0.0603 0 1 1 1100.51964 4399.05674 4399.06016 -0.78 -0.00085 759072.0 CID (Collision Induced Dissociation) MS2 0.0 40 31.0 60.0 103.8042 34139 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 30.5 29.9 26.0 37.2 28.5 31.9 33.4 34.9 26.9 30.3 Excluded by Method 5.47 1 0.0003003 6.567e-05 False High Sequest HT (A2) Unambiguous ESMCSTPAFPVSPETPYVK [K].eSmcSTPAFPVsPETPYVk.[T] N-Term(TMT6plex); M3(Oxidation); C4(Carbamidomethyl); S12(Phospho); K19(TMT6plex) 2 Q68CZ2 Q68CZ2; Q68CZ2-2 0 3 0.0154 0 1 1 894.42824 2681.27018 2681.26441 2.15 0.00192 368783.375 CID (Collision Induced Dissociation) MS2 0.0 80 32.2 60.0 104.5998 34438 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 31.2 37.1 22.8 30.2 33.0 35.7 32.6 32.4 31.3 35.3 3.89 1 0.0003003 0.0004659 False High Sequest HT (A2) Unambiguous GRAEGEWEDQEALDYFSDK [K].gRAEGEWEDQEALDYFsDk.[E] N-Term(TMT6plex); S17(Phospho); K19(TMT6plex) 3 Q9NYF8 Q9NYF8-3; Q9NYF8; Q9NYF8-2 1 3 0.1134 0 1 1 928.42332 2783.2554 2783.26019 -1.72 -0.0016 1271061.5 CID (Collision Induced Dissociation) MS2 0.0 40 61.4 42.675 104.0948 34249 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 57.2 67.1 49.5 64.9 50.5 70.3 69.0 58.0 67.6 60.0 Excluded by Method 4.94 1 0.0003003 3.249e-05 False High Sequest HT (A2) Unambiguous VSAEAEVAPVSPEVTQEVVEEHCASPEDK [K].vSAEAEVAPVsPEVTQEVVEEHcAsPEDk.[T] N-Term(TMT6plex); S11(Phospho); C23(Carbamidomethyl); S25(Phospho); K29(TMT6plex) 1 P46821 P46821 0 4 0.1536 0 1 1 935.93295 3740.70997 3740.70556 1.18 0.0011 715578.9375 CID (Collision Induced Dissociation) MS2 34.06585 20 88.9 60.0 104.2573 34309 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 91.2 81.4 75.8 97.3 72.6 92.7 106.0 94.2 92.2 85.3 Excluded by Method 5.73 1 0.0003003 0.0001424 False High Sequest HT (A2) Unambiguous GSLVGLVDYPDDEEEDEEEESSPRK [K].gSLVGLVDYPDDEEEDEEEESsPRk.[R] N-Term(TMT6plex); S22(Phospho); K25(TMT6plex) 4 Q5MIZ7 Q5MIZ7-3; Q5MIZ7; Q5MIZ7-2; Q5MIZ7-5 1 3 0.0037 0 1 1 1121.1805 3361.52696 3361.52486 0.63 0.0007 543435.8125 CID (Collision Induced Dissociation) MS2 10.97367 40 41.5 60.0 104.8749 34541 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 45.0 38.8 35.4 43.5 40.2 39.9 45.7 41.4 39.0 45.7 Excluded by Method 5.35 1 0.0003003 0.0004423 False High Sequest HT (A2) Unambiguous ASEVEEILDGNDEK [K].aSEVEEILDGNDEk.[Y] N-Term(TMT6plex); K14(TMT6plex) 2 Q12824 Q12824; Q12824-2 0 3 0.2175 0 1 1 669.34914 2006.03287 2006.033 -0.07 -5e-05 396393.9375 CID (Collision Induced Dissociation) MS2 42.52888 70 41.2 60.0 105.7383 34863 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 41.7 32.0 38.5 44.3 35.4 40.9 44.4 50.5 37.6 46.3 3.08 1 0.0003003 0.0002736 False High Sequest HT (A2) Unambiguous GLIVYCVTSPK [K].gLIVYcVTsPk.[K] N-Term(TMT6plex); C6(Carbamidomethyl); S9(Phospho); K11(TMT6plex) 2 Q8TEU7 Q8TEU7; Q8TEU7-4 0 3 0.1614 0 1 1 592.32397 1774.95737 1774.95787 -0.28 -0.00017 1260713.125 CID (Collision Induced Dissociation) MS2 4.659388 60 119.6 60.0 105.7102 34853 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 125.3 111.6 92.6 136.4 111.0 123.9 140.3 124.0 115.6 115.7 Excluded by Method 3.78 1 0.0003003 3.319e-05 False High Sequest HT (A2) Unambiguous GLINDPDVNNTFNINK [K].gLINDPDVNNTFNINk.[G] N-Term(TMT6plex); K16(TMT6plex) 1 P32449 P32449 0 3 0.5424 0 1 1 749.41056 2246.21711 2246.21812 -0.45 -0.00033 2314312.0 CID (Collision Induced Dissociation) MS2 9.597257 30 72.0 43.363 104.8589 34534 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 18.4 56.0 117.4 25.1 56.5 142.2 19.2 66.6 142.3 76.6 Excluded by Method 4.48 1 0.0003003 0.0001841 False High Sequest HT (A2) Unambiguous DEGIGSPDIWEDEK [K].dEGIGsPDIWEDEk.[A] N-Term(TMT6plex); S6(Phospho); K14(TMT6plex) 1 Q01664 Q01664 0 3 0.356 0 1 1 710.00125 2127.98918 2127.98877 0.2 0.00014 3867948.5 CID (Collision Induced Dissociation) MS2 1.839441 70 110.7 31.217 104.9527 34569 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 104.1 104.2 84.3 126.2 100.1 104.5 142.4 121.0 111.5 108.4 5.0 1 0.0003003 2.104e-05 False High Sequest HT (A2) Unambiguous LCAGIMITASHNPK [K].lcAGIMITAsHNPk.[Q] N-Term(TMT6plex); C2(Carbamidomethyl); S10(Phospho); K14(TMT6plex) 2 Q96G03 Q96G03-2; Q96G03 0 4 0.1154 0 1 1 513.52025 2051.05916 2051.05833 0.41 0.00021 1657234.0 CID (Collision Induced Dissociation) MS2 5.337653 90 33.8 60.0 91.8133 29633 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 38.6 34.9 27.2 29.5 31.5 35.9 40.3 35.0 34.0 30.8 3.12 1 0.0003003 0.0004001 False High Sequest HT (A2) Unambiguous LIDENATENGLAGSPK [K].lIDENATENGLAGsPk.[D] N-Term(TMT6plex); S14(Phospho); K16(TMT6plex) 1 P31374 P31374 0 3 0.538 0 1 1 723.0406 2167.10724 2167.1048 1.12 0.00081 1472561.125 CID (Collision Induced Dissociation) MS2 3.578086 80 111.4 60.0 91.402 29479 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 30.2 99.2 164.7 34.2 82.7 205.8 38.9 105.0 237.0 116.3 5.52 1 0.0003003 1.994e-07 False High Sequest HT (A2) Unambiguous VVDYSQFQESDDADEDYGRDSGPPTK [K].vVDYSQFQEsDDADEDYGRDSGPPTk.[K] N-Term(TMT6plex); S10(Phospho); K26(TMT6plex) 1 Q9H1E3 Q9H1E3 1 4 0.1559 0 1 1 865.38763 3458.52871 3458.53134 -0.76 -0.00066 1840425.75 CID (Collision Induced Dissociation) MS2 5.877563 60 54.5 27.68 91.3895 29474 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 57.3 54.9 51.0 46.7 55.1 53.2 58.3 61.1 57.8 50.1 Excluded by Method 6.22 1 0.0003003 1.597e-06 False High Sequest HT (A2) Unambiguous EQNVAFSPLK [K].eQNVAFsPLk.[R] N-Term(TMT6plex); S7(Phospho); K10(TMT6plex) 1 Q12882 Q12882 0 3 0.2528 0 1 1 557.63481 1670.88988 1670.89189 -1.2 -0.00067 754877.6875 CID (Collision Induced Dissociation) MS2 0.0 80 97.5 60.0 91.3295 29453 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 104.3 100.7 79.6 99.5 92.5 94.4 101.4 110.4 97.3 94.5 5.38 1 0.0003003 0.0004367 False High Sequest HT (A2) Unambiguous ALSYASLDK [K].aLsYASLDk.[E] N-Term(TMT6plex); S3(Phospho); K9(TMT6plex) 2 Q6R327 Q6R327; Q6R327-3 0 3 0.0302 0 1 1 502.60547 1505.80185 1505.80168 0.11 5e-05 856088.5625 CID (Collision Induced Dissociation) MS2 0.0 60 12.2 60.0 92.253 29801 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 14.6 14.1 10.8 11.5 10.7 14.0 11.6 14.6 8.8 11.7 Excluded by Method 2.98 1 0.0003003 0.0002557 False High Sequest HT (A2) Unambiguous LVQIAEEESDK [K].lVQIAEEEsDk.[E] N-Term(TMT6plex); S9(Phospho); K11(TMT6plex) 1 Q06810 Q06810 0 3 0.4645 0 1 1 600.31267 1798.92346 1798.92398 -0.29 -0.00017 821402.25 CID (Collision Induced Dissociation) MS2 4.374105 90 63.7 60.0 92.5112 29895 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 19.4 52.1 84.7 21.3 47.8 119.8 24.1 60.4 136.4 70.6 4.65 1 0.0003003 1.489e-05 False High Sequest HT (A2) Unambiguous FEDEGAGFEESSETGDYEEK [K].fEDEGAGFEESsETGDYEEk.[A] N-Term(TMT6plex); S12(Phospho); K20(TMT6plex) 1 P46821 P46821 0 4 0.0688 0 1 1 699.04759 2793.16852 2793.17043 -0.69 -0.00048 1092105.375 CID (Collision Induced Dissociation) MS2 8.321567 80 67.2 58.017 94.6655 30705 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 71.7 65.6 55.7 69.2 68.5 57.0 77.6 71.5 69.9 65.7 7.12 1 0.0003003 2.338e-06 False High Sequest HT (A2) Unambiguous QREMLMEDVGSEEEQEEEDEAPFQEK [K].qREmLMEDVGsEEEQEEEDEAPFQEk.[D] N-Term(TMT6plex); M4(Oxidation); S11(Phospho); K26(TMT6plex) 2 Q9H1E3 Q9H1E3; Q9H1E3-2 1 4 0.0019 0 1 1 924.65477 3695.59724 3695.6018 -1.24 -0.00114 1125463.875 CID (Collision Induced Dissociation) MS2 1.753056 40 33.4 42.388 94.5212 30650 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 35.3 28.2 26.3 38.4 33.0 39.6 34.5 32.2 31.2 35.4 Excluded by Method 5.24 1 0.0003003 0.0002456 False High Sequest HT (A2) Unambiguous VVDYSQFQESDDADEDYGRDSGPPTK [K].vVDYSQFQEsDDADEDYGRDSGPPTk.[K] N-Term(TMT6plex); S10(Phospho); K26(TMT6plex) 1 Q9H1E3 Q9H1E3 1 4 0.0518 0 1 1 865.38947 3458.53603 3458.53134 1.36 0.00117 800606.875 CID (Collision Induced Dissociation) MS2 0.0 60 23.4 60.0 93.9669 30442 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 17.5 21.3 19.3 22.1 22.1 25.4 22.6 25.1 29.2 29.3 Excluded by Method 7.53 1 0.0003003 4.73e-06 False High Sequest HT (A2) Unambiguous ITIADCGQLE [K].iTIADcGQLE.[-] N-Term(TMT6plex); C6(Carbamidomethyl) 2 P62937 P62937; P62937-2 0 2 0.6511 0 1 1 674.85267 1348.69807 1348.69798 0.06 4e-05 6605124.5 CID (Collision Induced Dissociation) MS2 12.05953 70 114.4 28.179 93.2026 30154 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 115.1 113.0 98.6 100.2 115.7 116.6 132.8 124.7 112.3 114.9 2.78 1 0.0003003 0.000437 False High Sequest HT (A2) Unambiguous VVDYSQFQESDDADEDYGRDSGPPTK [K].vVDYSQFQEsDDADEDYGRDSGPPTk.[K] N-Term(TMT6plex); S10(Phospho); K26(TMT6plex) 1 Q9H1E3 Q9H1E3 1 4 0.1181 0 1 1 865.38745 3458.52797 3458.53134 -0.97 -0.00084 693745.0 CID (Collision Induced Dissociation) MS2 0.0 70 36.3 45.714 92.6069 29931 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 27.6 32.0 33.8 34.0 35.5 36.7 39.4 44.5 38.0 41.0 5.76 1 0.0003003 2.883e-05 False High Sequest HT (A2) Unambiguous LFIIRGSPQQIDHAK [K].lFIIRGsPQQIDHAk.[Q] N-Term(TMT6plex); S7(Phospho); K15(TMT6plex) 1 Q92945 Q92945 1 4 0.4975 0 1 1 566.06992 2261.25785 2261.25716 0.31 0.00017 1032909.125 CID (Collision Induced Dissociation) MS2 1.842616 20 84.3 60.0 94.6835 30712 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 75.8 74.3 65.0 83.9 81.5 90.5 97.3 88.1 89.7 96.8 Excluded by Method 4.06 1 0.0003003 0.0003325 False High Sequest HT (A2) Unambiguous YGGPYHIGGSPFK [K].yGGPYHIGGsPFk.[A] N-Term(TMT6plex); S10(Phospho); K13(TMT6plex) 2 P21333 P21333; P21333-2 0 3 0.4212 0 1 1 639.99365 1917.96639 1917.96646 -0.03 -2e-05 1249089.625 CID (Collision Induced Dissociation) MS2 5.145415 30 73.3 55.857 89.6067 28798 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 76.3 76.6 56.1 79.9 72.0 72.1 79.9 77.5 69.5 72.8 Excluded by Method 3.11 1 0.0003003 0.0002811 False High Sequest HT (A2) Unambiguous SIFDTDDDD [K].sIFDtDDDD.[-] N-Term(TMT6plex); T5(Phospho) 1 P36054 P36054 0 2 0.5 0 1 1 676.26087 1351.51447 1351.514 0.35 0.00023 1360166.625 CID (Collision Induced Dissociation) MS2 13.01208 80 136.2 60.0 89.5859 28790 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 41.3 111.3 219.6 40.0 110.5 252.5 49.6 120.1 282.9 134.0 3.58 1 0.0003003 7.167e-05 False High Sequest HT (A2) Unambiguous IELSDDSDVEVHPNVDKK [K].iELsDDsDVEVHPNVDkk.[S] N-Term(TMT6plex); S4(Phospho); S7(Phospho); K17(TMT6plex); K18(TMT6plex) 1 P06101 P06101 1 4 0.6141 0 1 1 722.35951 2886.41619 2886.41421 0.69 0.0005 983955.3125 CID (Collision Induced Dissociation) MS2 0.0 0 124.1 60.0 89.6184 28802 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 41.0 114.6 183.2 48.5 110.0 208.9 40.1 129.9 231.5 133.3 Excluded by Method 5.83 1 0.0003003 5.568e-05 False High Sequest HT (A2) Unambiguous ALSYASLDK [K].aLSYAsLDk.[E] N-Term(TMT6plex); S6(Phospho); K9(TMT6plex) 2 Q6R327 Q6R327; Q6R327-3 0 2 0.1241 0 1 1 753.40463 1505.80198 1505.80168 0.2 0.00015 2525719.0 CID (Collision Induced Dissociation) MS2 9.393049 20 110.6 58.044 89.2905 28679 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 111.2 108.4 88.2 108.5 110.2 112.6 113.7 119.6 126.4 107.7 Excluded by Method 3.95 1 0.0003003 0.0003759 False High Sequest HT (A2) Unambiguous EEPLSEEEPCTSTAIASPEK [K].eEPLSEEEPctSTAIAsPEk.[K] N-Term(TMT6plex); C10(Carbamidomethyl); T11(Phospho); S17(Phospho); K20(TMT6plex) 1 Q9Y2X3 Q9Y2X3 0 3 0.0883 0 1 1 941.41927 2822.24326 2822.24963 -2.26 -0.00212 311295.40625 CID (Collision Induced Dissociation) MS2 0.0 30 224.4 60.0 89.2539 28666 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 229.2 212.3 190.6 233.8 219.7 213.2 253.3 224.4 241.4 225.8 Excluded by Method 4.87 1 0.0003003 0.0003282 False High Sequest HT (A2) Unambiguous EEEPILAPSVK [K].eEEPILAPsVk.[D] N-Term(TMT6plex); S9(Phospho); K11(TMT6plex) 1 P05750 P05750 0 3 0.4704 0 1 1 583.98634 1749.94446 1749.94399 0.27 0.00016 1517438.125 CID (Collision Induced Dissociation) MS2 34.61556 80 177.0 40.137 89.2268 28655 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 48.0 143.1 286.5 59.7 145.2 321.8 60.5 167.0 366.7 171.3 3.04 1 0.0003003 0.0002359 False High Sequest HT (A2) Unambiguous IGEGTYGVVYK [K].iGEGTyGVVYk.[AG] N-Term(TMT6plex); Y6(Phospho); K11(TMT6plex) 5 P24941 P24941; P06493; Q00526; P24941-2; P06493-2 0 2 0.0278 0 1 1 862.45758 1723.90789 1723.90721 0.4 0.00034 8316545.5 CID (Collision Induced Dissociation) MS2 9.253338 90 70.9 19.107 89.0343 28580 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 72.7 69.9 57.6 71.1 69.0 60.6 87.5 74.8 67.5 78.4 4.32 1 0.0003003 6.385e-06 False High Sequest HT (A2) Unambiguous ASGAFTGENSVDQIK [K].asGAFTGENSVDQIk.[D] N-Term(TMT6plex); S2(Phospho); K15(TMT6plex) 1 P00942 P00942 0 3 0.0699 0 1 1 688.01415 2062.02791 2062.02582 1.01 0.0007 1765817.75 CID (Collision Induced Dissociation) MS2 30.71227 50 100.3 60.0 89.3086 28686 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 51.6 79.3 135.1 55.1 87.4 155.6 64.1 101.6 169.6 103.3 Excluded by Method 3.29 1 0.0003003 0.0003906 False High Sequest HT (A2) Unambiguous DSGSDEDFLMEDDDDSDYGSSK [K].dSGsDEDFLmEDDDDSDYGSSk.[K] N-Term(TMT6plex); S4(Phospho); M10(Oxidation); K22(TMT6plex) 2 Q9H1E3 Q9H1E3; Q9H1E3-2 0 3 0.0461 0 1 1 995.06147 2983.16986 2983.16 3.3 0.00329 278625.65625 CID (Collision Induced Dissociation) MS2 63.09497 60 20.0 60.0 89.8456 28887 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 21.4 16.4 15.0 22.5 20.6 19.6 22.9 21.2 22.8 17.7 Excluded by Method 4.77 1 0.0003003 2.906e-05 False High Sequest HT (A2) Unambiguous TMFAQVESDDEEAK [K].tMFAQVEsDDEEAk.[N] N-Term(TMT6plex); S8(Phospho); K14(TMT6plex) 1 Q9NW82 Q9NW82 0 3 0.4699 0 1 1 713.33016 2137.97593 2137.97649 -0.26 -0.00019 2075479.375 CID (Collision Induced Dissociation) MS2 6.700909 50 142.4 57.101 90.7708 29239 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 142.0 141.5 106.5 151.5 127.8 132.2 181.7 157.2 135.9 148.0 Excluded by Method 5.64 1 0.0003003 3.517e-06 False High Sequest HT (A2) Unambiguous KSPSEARQDVDLCLVSSCEYK [K].ksPSEARQDVDLcLVSScEYk.[H] N-Term(TMT6plex); K1(TMT6plex); S2(Phospho); C13(Carbamidomethyl); C18(Carbamidomethyl); K21(TMT6plex) 1 P46821 P46821 2 4 0.1199 0 1 1 810.40736 3238.60762 3238.60923 -0.5 -0.0004 2086606.75 CID (Collision Induced Dissociation) MS2 0.0 50 77.2 55.734 90.7465 29229 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 74.9 67.5 70.4 75.0 77.2 81.1 85.3 75.7 86.8 77.7 Excluded by Method 4.42 1 0.0003003 3.061e-05 False High Sequest HT (A2) Unambiguous DSVDDNENSDDGLDIPK [K].dSVDDNENsDDGLDIPk.[N] N-Term(TMT6plex); S9(Phospho); K17(TMT6plex) 1 Q06631 Q06631 0 2 0.2978 0 1 1 1193.53807 2386.06887 2386.06993 -0.45 -0.00053 847867.9375 CID (Collision Induced Dissociation) MS2 0.0 40 29.7 60.0 90.5451 29156 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 8.8 19.9 41.3 13.1 28.7 48.8 9.7 26.6 65.7 34.8 Excluded by Method 4.5 1 0.0003003 0.000229 False High Sequest HT (A2) Unambiguous FACQIDIPNEENNNASK [K].fAcQIDIPNEENNNASk.[D] N-Term(TMT6plex); C3(Carbamidomethyl); K17(TMT6plex) 1 P06105 P06105 0 3 0.5191 0 1 1 808.07399 2422.20742 2422.20729 0.05 4e-05 905891.75 CID (Collision Induced Dissociation) MS2 6.14648 40 89.1 60.0 90.3231 29071 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 22.6 72.6 136.9 28.8 72.6 168.3 27.8 79.9 184.9 96.6 Excluded by Method 3.66 1 0.0003003 4.051e-05 False High Sequest HT (A2) Unambiguous LWNLQNANNK [K].lWNLQNANNk.[S] N-Term(TMT6plex); K10(TMT6plex) 1 P16649 P16649 0 3 0.5119 0 1 1 558.32213 1672.95183 1672.95351 -1.0 -0.00056 678767.0625 CID (Collision Induced Dissociation) MS2 45.90307 100 213.5 60.0 90.2238 29032 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 54.9 175.7 331.2 72.7 169.1 423.2 73.5 200.5 413.5 220.3 4.63 1 0.0003003 6.185e-05 False High Sequest HT (A2) Unambiguous GILAADESTGSIAK [K].gILAADESTGsIAk.[R] N-Term(TMT6plex); S11(Phospho); K14(TMT6plex) 2 P04075 P04075; P04075-2 0 3 0.0833 0 1 1 624.33559 1870.99221 1870.99273 -0.28 -0.00017 2900968.0 CID (Collision Induced Dissociation) MS2 1.487488 40 129.9 50.9 90.1007 28984 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 134.6 110.7 109.6 137.9 127.0 137.2 141.6 137.8 140.1 122.3 Excluded by Method 3.12 1 0.0003003 1.857e-05 False High Sequest HT (A2) Unambiguous IELSDDSDVEVHPNVDK [K].iELSDDsDVEVHPNVDk.[K] N-Term(TMT6plex); S7(Phospho); K17(TMT6plex) 1 P06101 P06101 0 3 0.0632 0 1 1 817.06812 2449.1898 2449.18999 -0.07 -6e-05 2204523.75 CID (Collision Induced Dissociation) MS2 38.66046 20 34.1 60.0 90.2958 29060 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 9.3 31.4 52.1 7.5 24.0 64.2 12.8 37.7 68.7 33.4 Excluded by Method 7.12 1 0.0003003 5.476e-08 False High Sequest HT (A2) Unambiguous IEEVLSPEGSPSK [K].iEEVLSPEGsPSk.[S] N-Term(TMT6plex); S10(Phospho); K13(TMT6plex) 2 Q9UEY8 Q9UEY8-2; Q9UEY8 0 3 0.154 0 1 1 637.33588 1909.99308 1909.9924 0.36 0.00023 1023084.625 CID (Collision Induced Dissociation) MS2 15.73032 30 234.4 60.0 98.4284 32121 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 219.7 227.8 177.4 235.8 226.4 229.0 283.1 256.9 252.6 235.2 Excluded by Method 4.74 1 0.0003003 0.0001719 False High Sequest HT (A2) Unambiguous DGVLTLANNVTPAK [K].dGVLTLANNVtPAk.[D] N-Term(TMT6plex); T11(Phospho); K14(TMT6plex) 2 P27816 P27816; P27816-6 0 3 0.5782 0 1 1 651.02733 1951.06742 1951.06656 0.44 0.00029 2650820.0 CID (Collision Induced Dissociation) MS2 35.93436 90 179.9 39.746 97.9379 31937 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 218.7 183.5 151.9 187.6 183.6 165.4 203.5 179.7 158.4 167.1 5.69 1 0.0003003 8.302e-07 False High Sequest HT (A2) Unambiguous EEDNIDEDEYEEEGEGEESDFDEYK [K].eEDNIDEDEYEEEGEGEEsDFDEYk.[D] N-Term(TMT6plex); S19(Phospho); K25(TMT6plex) 1 Q06839 Q06839 0 3 0.1757 0 1 1 1194.47716 3581.41692 3581.40525 3.26 0.00389 544177.0625 CID (Collision Induced Dissociation) MS2 48.04217 80 13.4 60.0 97.8162 31892 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 2.8 12.5 20.4 2.6 9.0 24.3 7.1 13.1 29.3 12.9 5.52 1 0.0003003 4.311e-06 False High Sequest HT (A2) Unambiguous AAEAGGAEEQYGFLTTPTK [K].aAEAGGAEEQYGFLTtPTk.[Q] N-Term(TMT6plex); T16(Phospho); K19(TMT6plex) 1 P46821 P46821 0 3 0.0304 0 1 1 827.07702 2479.2165 2479.21581 0.28 0.00023 3672508.0 CID (Collision Induced Dissociation) MS2 5.833375 60 55.0 29.574 97.6356 31823 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 55.1 54.0 47.9 61.1 54.2 50.1 62.9 55.2 54.6 55.1 Excluded by Method 4.27 1 0.0003003 7.611e-05 False High Sequest HT (A2) Unambiguous FEGSDDDENHPLPPLNVAK [K].fEGsDDDENHPLPPLNVAk.[G] N-Term(TMT6plex); S4(Phospho); K19(TMT6plex) 1 P13185 P13185 0 3 0.6252 0 1 1 878.0955 2632.27196 2632.26963 0.88 0.00077 1542747.375 CID (Collision Induced Dissociation) MS2 52.70231 20 69.9 60.0 100.3455 32837 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 15.7 54.1 115.5 23.2 57.5 139.8 26.1 61.6 136.7 69.0 Excluded by Method 6.51 1 0.0003003 7.56e-07 False High Sequest HT (A2) Unambiguous GDNITLLQSVSN [K].gDNITLLQSVSN.[-] N-Term(TMT6plex) 1 P62304 P62304 0 2 0.5729 0 1 1 745.40672 1489.80615 1489.80595 0.14 0.0001 1326406.875 CID (Collision Induced Dissociation) MS2 18.9098 80 164.1 60.0 99.2835 32440 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 166.5 170.6 133.9 159.1 147.8 181.5 169.6 188.9 166.9 156.3 2.88 1 0.0003003 5.105e-05 False High Sequest HT (A2) Unambiguous IEEVLSPEGSPSKSPSK [K].iEEVLSPEGsPskSPSk.[K] N-Term(TMT6plex); S10(Phospho); S12(Phospho); K13(TMT6plex); K17(TMT6plex) 2 Q9UEY8 Q9UEY8-2; Q9UEY8 1 4 0.0693 0 1 1 655.33897 2618.33403 2618.33344 0.23 0.00015 440961.53125 CID (Collision Induced Dissociation) MS2 5.243401 30 123.0 60.0 99.137 32386 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 116.7 127.6 85.6 151.8 124.9 112.3 144.3 140.2 123.0 104.0 Excluded by Method 5.34 1 0.0003003 0.0002097 False High Sequest HT (A2) Unambiguous FQYIAISQSDADSESCK [K].fQYIAISQSDADSESck.[M] N-Term(TMT6plex); C16(Carbamidomethyl); K17(TMT6plex) 1 P38013 P38013 0 3 0.6477 0 1 1 803.0671 2407.18675 2407.18516 0.66 0.00053 1934921.75 CID (Collision Induced Dissociation) MS2 4.569589 90 74.2 54.836 97.432 31743 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 20.7 54.5 113.5 22.2 63.3 144.6 25.9 71.7 146.1 79.2 5.96 1 0.0003003 8.481e-08 False High Sequest HT (A2) Unambiguous EVQSILTQEVDNNNK [K].eVQSILTQEVDNNNk.[T] N-Term(TMT6plex); K15(TMT6plex) 1 P47119 P47119 0 4 0.4844 0 1 1 548.05041 2189.17982 2189.1814 -0.72 -0.00039 313988.03125 CID (Collision Induced Dissociation) MS2 0.0 60 57.1 60.0 96.0402 31221 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 15.5 46.0 88.5 23.3 51.8 99.9 25.5 49.9 111.0 59.6 Excluded by Method 5.12 1 0.0003003 1.743e-05 False High Sequest HT (A2) Unambiguous KTESPLENVAELK [K].kTEsPLENVAELk.[K] N-Term(TMT6plex); K1(TMT6plex); S4(Phospho); K13(TMT6plex) 1 P35732 P35732 1 3 0.0726 0 1 1 742.4185 2225.24095 2225.23973 0.55 0.00041 1423498.25 CID (Collision Induced Dissociation) MS2 0.0 100 163.7 60.0 95.904 31171 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 45.1 137.7 235.6 64.5 137.9 306.9 60.4 141.9 336.9 170.2 5.65 1 0.0003003 5.493e-06 False High Sequest HT (A2) Unambiguous EKPLNSDQEDVFISEK [K].ekPLNsDQEDVFISEk.[T] N-Term(TMT6plex); K2(TMT6plex); S6(Phospho); K16(TMT6plex) 1 Q04279 Q04279 0 3 0.5028 0 1 1 882.4614 2645.36966 2645.36784 0.69 0.00061 1305894.25 CID (Collision Induced Dissociation) MS2 14.46228 20 48.4 60.0 95.7639 31119 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 36.2 47.8 53.0 33.7 51.8 62.1 36.7 51.1 66.2 45.3 Excluded by Method 3.58 1 0.0003003 0.0004012 False High Sequest HT (A2) Unambiguous QLPLEPESPSGQVGPRPAPPQEESPSSEAK [K].qLPLEPESPsGQVGPRPAPPQEEsPSSEAk.[S] N-Term(TMT6plex); S10(Phospho); S24(Phospho); K30(TMT6plex) 3 Q3KQU3 Q3KQU3-2; Q3KQU3-4; Q3KQU3 0 4 0.3936 0 1 1 936.70437 3743.79566 3743.79709 -0.38 -0.00036 529581.375 CID (Collision Induced Dissociation) MS2 0.0 20 61.4 60.0 95.3612 30966 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 63.1 62.2 52.3 68.9 55.5 59.6 76.1 58.6 58.1 59.3 Excluded by Method 6.25 1 0.0003003 1.865e-05 False High Sequest HT (A2) Unambiguous IGEGTYGVVYK [K].iGEGtyGVVYk.[AG] N-Term(TMT6plex); T5(Phospho); Y6(Phospho); K11(TMT6plex) 5 P24941 P24941; P06493; Q00526; P24941-2; P06493-2 0 2 0.2907 0 1 1 902.4405 1803.87373 1803.87354 0.1 9e-05 9031624.0 CID (Collision Induced Dissociation) MS2 3.798547 40 229.5 19.295 95.6305 31067 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 238.2 242.6 183.8 251.8 217.9 210.9 258.5 238.3 241.1 212.2 Excluded by Method 4.92 1 0.0003003 1.03e-05 False High Sequest HT (A2) Unambiguous VVDYSQFQESDDADEDYGRDSGPPTK [K].vVDYSQFQEsDDADEDYGRDSGPPTk.[K] N-Term(TMT6plex); S10(Phospho); K26(TMT6plex) 1 Q9H1E3 Q9H1E3 1 4 0.1368 0 1 1 865.38812 3458.53066 3458.53134 -0.2 -0.00017 525381.5625 CID (Collision Induced Dissociation) MS2 17.59451 50 17.8 60.0 97.3022 31694 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 18.2 19.4 20.3 15.9 19.3 21.2 12.1 17.2 14.3 19.7 Excluded by Method 5.7 1 0.0003003 2.53e-05 False High Sequest HT (A2) Unambiguous DESCYDNAEAAFSDDEEDLNSK [K].dEScYDNAEAAFsDDEEDLNSk.[G] N-Term(TMT6plex); C4(Carbamidomethyl); S13(Phospho); K22(TMT6plex) 4 Q68E01 Q68E01; Q68E01-4; Q68E01-2; Q68E01-3 0 3 0.3153 0 1 1 1021.42173 3062.25063 3062.24982 0.26 0.00027 1363555.75 CID (Collision Induced Dissociation) MS2 0.0 70 55.4 60.0 97.278 31684 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 56.5 53.2 38.1 66.3 48.7 64.9 60.0 61.3 52.7 52.0 8.31 1 0.0003003 5.746e-10 False High Sequest HT (A2) Unambiguous DLSSLYSEK [K].dLSSLYsEk.[K] N-Term(TMT6plex); S7(Phospho); K9(TMT6plex) 1 P25558 P25558 0 3 0.2818 0 1 1 527.27212 1579.80181 1579.80208 -0.17 -9e-05 644808.6875 CID (Collision Induced Dissociation) MS2 38.00534 70 82.2 60.0 96.8923 31538 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 23.3 56.0 124.4 24.8 63.3 158.3 37.1 75.8 167.2 91.5 3.69 1 0.0003003 0.0003292 False High Sequest HT (A2) Unambiguous IGEGTYGVVYK [K].iGEGtYGVVYk.[AG] N-Term(TMT6plex); T5(Phospho); K11(TMT6plex) 5 P24941 P24941; P06493; Q00526; P24941-2; P06493-2 0 2 0.0928 0 1 1 862.45703 1723.90679 1723.90721 -0.24 -0.00021 29905912.0 CID (Collision Induced Dissociation) MS2 9.647584 50 157.5 7.036 96.5307 31405 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 160.8 160.6 123.5 165.4 165.9 150.5 183.4 159.7 148.3 157.0 Excluded by Method 4.31 1 0.0003003 0.0005007 False High Sequest HT (A2) Unambiguous SPQPPVEEEDEHFDDTVVCLDTYNCDLHFK [K].sPQPPVEEEDEHFDDTVVcLDTYNcDLHFk.[I] N-Term(TMT6plex); S1(Phospho); C19(Carbamidomethyl); C25(Carbamidomethyl); K30(TMT6plex) 2 Q00839 Q00839-2; Q00839 0 4 0.4208 0 1 1 1044.22199 4173.86613 4173.84991 3.88 0.00405 353910.8125 CID (Collision Induced Dissociation) MS2 35.87197 60 20.9 60.0 120.1259 40199 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 23.1 24.7 17.3 20.3 19.8 21.9 22.9 22.2 17.5 19.3 Excluded by Method 9.98 1 0.0003003 9.731e-10 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVssDEEF.[-] N-Term(TMT6plex); S8(Phospho); S9(Phospho) 1 P16521 P16521 0 2 0.1148 0 1 1 925.35459 1849.70191 1849.70195 -0.02 -2e-05 7471839.0 CID (Collision Induced Dissociation) MS2 0.0 40 160.2 25.998 120.2807 40258 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 39.5 118.5 266.0 44.8 118.0 322.1 52.7 155.7 321.7 162.9 Excluded by Method 4.53 1 0.0003003 2.428e-05 False High Sequest HT (A2) Unambiguous RVTFGEDLSPEVFDESLPANTPLRK [K].rVTFGEDLsPEVFDESLPANTPLRk.[G] N-Term(TMT6plex); S9(Phospho); K25(TMT6plex) 1 Q69YH5 Q69YH5 2 4 0.2739 0 1 1 839.68869 3355.73295 3355.73394 -0.3 -0.00025 803875.6875 CID (Collision Induced Dissociation) MS2 14.65371 30 36.2 60.0 119.742 40059 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 29.3 34.9 41.9 25.8 33.0 45.2 35.5 41.7 42.0 32.9 Excluded by Method 4.49 1 0.0003003 4.79e-05 False High Sequest HT (A2) Unambiguous IVVVRAEELNISGEFFRNK [K].iVVVRAEELNISGEFFRNk.[L] N-Term(TMT6plex); K19(TMT6plex) 1 P26784 P26784 2 4 0.6067 0 1 1 670.39046 2678.54001 2678.53939 0.23 0.00015 1066661.25 CID (Collision Induced Dissociation) MS2 12.21166 40 74.5 60.0 119.7268 40053 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 18.1 57.3 115.8 23.8 61.1 164.5 20.1 68.4 150.9 65.2 Excluded by Method 5.39 1 0.0003003 1.586e-06 False High Sequest HT (A2) Unambiguous LAALESPPPVLK [K].lAALEsPPPVLk.[A] N-Term(TMT6plex); S6(Phospho); K12(TMT6plex) 1 P21576 P21576 0 3 0.3782 0 1 1 591.68292 1773.03422 1773.03275 0.83 0.00049 669517.375 CID (Collision Induced Dissociation) MS2 0.0 70 132.0 60.0 119.6785 40036 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 39.3 107.9 214.9 48.9 110.8 249.1 53.3 122.1 241.2 132.7 3.86 1 0.0003003 0.0003112 False High Sequest HT (A2) Unambiguous SFEERVETTVTSLK [K].sFEERVETTVTSLk.[T] N-Term(TMT6plex); S1(Phospho); K14(TMT6plex) 2 Q16890 Q16890; Q16890-5 1 3 0.332 0 1 1 722.04872 2164.13159 2164.13029 0.6 0.00044 1266226.875 CID (Collision Induced Dissociation) MS2 7.557006 40 45.6 53.259 119.6629 40030 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 41.6 48.6 37.6 49.6 40.5 45.7 50.8 46.8 43.6 51.7 Excluded by Method 5.15 1 0.0003003 1.536e-05 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVSsDEEF.[-] N-Term(TMT6plex); S9(Phospho) 1 P16521 P16521 0 2 0.0119 0 1 1 885.36985 1769.73242 1769.73562 -1.81 -0.0016 966042.625 CID (Collision Induced Dissociation) MS2 10.99616 50 69.0 60.0 119.4382 39948 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 21.6 57.1 115.8 16.9 51.4 143.4 17.3 58.9 142.1 65.2 Excluded by Method 4.2 1 0.0003003 7.096e-05 False High Sequest HT (A2) Unambiguous GVNLPGTDVDLPALSEK [K].gVNLPGTDVDLPALSEk.[D] N-Term(TMT6plex); K17(TMT6plex) 1 P00549 P00549 0 3 0.4789 0 1 1 728.41571 2183.23258 2183.23237 0.1 7e-05 645574.5625 CID (Collision Induced Dissociation) MS2 28.90212 50 45.1 60.0 119.7397 40058 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 17.0 41.5 59.6 22.1 42.4 82.6 17.2 37.8 82.6 48.2 Excluded by Method 4.51 1 0.0003003 0.0002952 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVSsDEEF.[-] N-Term(TMT6plex); S9(Phospho) 1 P16521 P16521 0 2 0.0289 0 1 1 885.36985 1769.73242 1769.73562 -1.81 -0.0016 739476.5625 CID (Collision Induced Dissociation) MS2 0.0 50 88.3 60.0 120.4958 40337 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 22.9 73.2 142.3 30.3 69.2 171.9 30.4 72.2 176.1 94.5 Excluded by Method 3.8 1 0.0003003 0.0003831 False High Sequest HT (A2) Unambiguous DVLDIEQFSTVK [K].dVLDIEQFsTVk.[G] N-Term(TMT6plex); S9(Phospho); K12(TMT6plex) 4 P34947 P34947; P43250; P43250-2; P43250-3 0 3 0.003 0 1 1 644.67608 1932.01368 1932.01313 0.28 0.00018 1261616.125 CID (Collision Induced Dissociation) MS2 3.219556 50 160.5 60.0 121.5062 40706 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 164.7 163.3 131.7 180.3 141.6 158.2 184.7 160.9 158.6 161.1 Excluded by Method 3.36 1 0.0003003 2.047e-05 False High Sequest HT (A2) Unambiguous SPQPPVEEEDEHFDDTVVCLDTYNCDLHFK [K].sPQPPVEEEDEHFDDTVVcLDTYNcDLHFk.[I] N-Term(TMT6plex); S1(Phospho); C19(Carbamidomethyl); C25(Carbamidomethyl); K30(TMT6plex) 2 Q00839 Q00839-2; Q00839 0 4 0.4925 0 1 1 1044.21845 4173.85197 4173.84991 0.49 0.00051 761676.375 CID (Collision Induced Dissociation) MS2 9.543482 60 13.1 60.0 121.3747 40655 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 13.2 15.0 9.3 12.2 12.6 13.5 15.8 12.9 13.8 12.4 Excluded by Method 7.35 1 0.0003003 1.772e-07 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVssDEEF.[-] N-Term(TMT6plex); S8(Phospho); S9(Phospho) 1 P16521 P16521 0 2 0.1269 0 1 1 925.35453 1849.70179 1849.70195 -0.09 -8e-05 7410133.0 CID (Collision Induced Dissociation) MS2 1.419437 40 177.3 60.0 121.3703 40654 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 39.0 139.4 286.9 45.9 143.1 365.2 54.0 168.8 349.5 180.7 Excluded by Method 4.49 1 0.0003003 8.436e-06 False High Sequest HT (A2) Unambiguous SPSFASEWDEIEK [K].sPSFAsEWDEIEk.[I] N-Term(TMT6plex); S6(Phospho); K13(TMT6plex) 1 Q92625 Q92625 0 3 0.0021 0 1 1 688.33044 2062.97676 2062.97747 -0.35 -0.00024 3410560.75 CID (Collision Induced Dissociation) MS2 1.221672 40 164.8 32.338 119.3623 39919 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 175.9 164.9 133.9 173.6 144.9 169.8 188.9 169.3 169.1 158.0 Excluded by Method 4.71 1 0.0003003 0.0004316 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVssDEEF.[-] N-Term(TMT6plex); S8(Phospho); S9(Phospho) 1 P16521 P16521 0 2 0.1269 0 1 1 925.35484 1849.7024 1849.70195 0.24 0.00022 4440161.0 CID (Collision Induced Dissociation) MS2 0.0 40 193.9 55.118 118.081 39441 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 52.1 158.9 310.7 53.6 162.9 372.1 72.0 176.4 383.4 197.0 Excluded by Method 4.65 1 0.0003003 4.022e-05 False High Sequest HT (A2) Unambiguous SGLELTELQNMTVPEDDNISNDSNDFTEVENGQINSK [K].sGLELTELQNmTVPEDDNIsNDsNDFTEVENGQINSk.[F] N-Term(TMT6plex); M11(Oxidation); S20(Phospho); S23(Phospho); K37(TMT6plex) 1 Q9H2H9 Q9H2H9 0 4 0.2813 0 1 1 1183.53735 4731.12759 4731.09459 6.98 0.00825 638900.5625 CID (Collision Induced Dissociation) MS2 0.0 70 6.3 60.0 118.0192 39418 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 5.9 6.6 9.2 6.5 3.7 3.1 7.5 7.8 3.5 9.1 4.55 1 0.0003003 0.0004608 False High Sequest HT (A2) Unambiguous ALIVEPSRELAEQTLNNIK [K].aLIVEPSRELAEQTLNNIk.[Q] N-Term(TMT6plex); K19(TMT6plex) 3 Q92499 Q92499; Q92499-3; Q92499-2 1 4 0.1137 0 1 1 649.88231 2596.5074 2596.50742 -0.01 0.0 838703.875 CID (Collision Induced Dissociation) MS2 8.707593 30 36.2 48.977 117.7481 39317 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 37.6 40.8 30.4 32.2 35.4 36.1 35.6 42.2 34.0 37.8 Excluded by Method 2.99 1 0.0003003 0.0002558 False High Sequest HT (A2) Unambiguous NIEDVIAQGIGK [K].nIEDVIAQGIGk.[L] N-Term(TMT6plex); K12(TMT6plex) 1 P05387 P05387 0 2 0.6325 0 1 1 858.0088 1715.01032 1715.01036 -0.02 -2e-05 1296291.125 CID (Collision Induced Dissociation) MS2 6.719385 100 73.9 60.0 118.0969 39447 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 79.2 71.4 63.1 77.6 69.7 85.7 83.0 78.3 68.0 62.7 4.68 1 0.0003003 6.138e-08 False High Sequest HT (A2) Unambiguous KGDLVNENEDLEELDIEEEYSD [K].kGDLVNENEDLEELDIEEEYsD.[-] N-Term(TMT6plex); K1(TMT6plex); S21(Phospho) 1 Q03771 Q03771 1 3 0.0343 0 1 1 1045.81098 3135.41838 3135.41827 0.03 4e-05 298005.875 CID (Collision Induced Dissociation) MS2 51.13874 60 30.3 60.0 117.6054 39263 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 9.6 24.7 43.8 14.4 23.6 51.5 15.4 31.0 61.2 27.7 Excluded by Method 3.5 1 0.0003003 9.435e-05 False High Sequest HT (A2) Unambiguous FPEFCSSPSPPVEVK [K].fPEFcSSPsPPVEVk.[I] N-Term(TMT6plex); C5(Carbamidomethyl); S9(Phospho); K15(TMT6plex) 4 Q68E01 Q68E01; Q68E01-4; Q68E01-2; Q68E01-3 0 3 0.2327 0 1 1 749.03891 2245.10219 2245.10163 0.25 0.00019 4016326.75 CID (Collision Induced Dissociation) MS2 2.348646 30 84.0 24.852 117.5378 39238 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 80.3 86.7 66.7 89.1 72.8 96.5 87.1 93.4 85.0 82.1 Excluded by Method 5.07 1 0.0003003 2.275e-05 False High Sequest HT (A2) Unambiguous GADFLVTEVENGGSLGSK [K].gADFLVTEVENGGSLGSk.[K] N-Term(TMT6plex); K18(TMT6plex) 3 P14618 P14618-2; P14618-3; P14618 0 3 0.5349 0 1 1 746.73905 2238.2026 2238.2018 0.36 0.00027 740379.5625 CID (Collision Induced Dissociation) MS2 8.832785 60 84.8 60.0 117.4914 39223 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 91.1 81.7 74.5 85.2 80.6 86.6 85.6 90.6 84.0 88.1 Excluded by Method 4.3 1 0.0003003 1.106e-06 False High Sequest HT (A2) Unambiguous ESDQTLAALLSPK [K].eSDQTLAALLsPk.[E] N-Term(TMT6plex); S11(Phospho); K13(TMT6plex) 9 P18583 P18583; P18583-4; P18583-6; P18583-5; P18583-10; P18583-9; P18583-2; P18583-3; P18583-7 0 3 0.5011 0 1 1 637.67963 1911.02433 1911.02403 0.15 0.0001 2512514.25 CID (Collision Induced Dissociation) MS2 10.36249 40 328.1 60.0 117.449 39206 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 353.1 330.1 258.4 375.8 311.7 294.8 386.4 340.9 337.4 292.6 Excluded by Method 4.63 1 0.0003003 5.952e-06 False High Sequest HT (A2) Unambiguous AYEPDLSDADFEGIEASPK [K].aYEPDLsDADFEGIEASPk.[L] N-Term(TMT6plex); S7(Phospho); K19(TMT6plex) 1 P49687 P49687 0 3 0.076 0 1 1 864.74329 2592.21531 2592.21587 -0.22 -0.00019 1242308.375 CID (Collision Induced Dissociation) MS2 16.48241 40 44.2 60.0 117.3952 39187 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 12.8 33.4 70.1 13.4 32.6 87.2 16.1 35.6 101.8 38.7 Excluded by Method 5.13 1 0.0003003 1.223e-05 False High Sequest HT (A2) Unambiguous TFVLTPELSPGK [K].tFVLTPELsPGk.[L] N-Term(TMT6plex); S9(Phospho); K12(TMT6plex) 1 Q96DY7 Q96DY7 0 3 0.4972 0 1 1 609.6738 1827.00686 1827.00692 -0.03 -2e-05 1126280.0 CID (Collision Induced Dissociation) MS2 3.67196 50 96.6 57.323 117.6368 39275 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 90.2 100.1 70.1 110.2 93.6 88.4 112.7 99.8 99.5 101.9 Excluded by Method 5.29 1 0.0003003 0.000162 False High Sequest HT (A2) Unambiguous EVDPSTGELQSLQMPESEGPSSLDPSQEGPTGLK [K].eVDPSTGELQSLQMPESEGPSsLDPSQEGPTGLk.[E] N-Term(TMT6plex); S22(Phospho); K34(TMT6plex) 2 Q13501 Q13501-2; Q13501 0 4 0.0117 0 1 1 1016.98698 4064.92609 4064.92994 -0.95 -0.00096 1600245.5 CID (Collision Induced Dissociation) MS2 2.371728 40 28.4 60.0 119.1896 39855 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 28.4 28.5 21.9 26.2 24.4 26.8 32.4 33.2 32.6 29.6 Excluded by Method 5.99 1 0.0003003 1.864e-06 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVssDEEF.[-] N-Term(TMT6plex); S8(Phospho); S9(Phospho) 1 P16521 P16521 0 2 0.1258 0 1 1 925.35453 1849.70179 1849.70195 -0.09 -8e-05 4422951.0 CID (Collision Induced Dissociation) MS2 2.660615 40 227.1 46.098 119.183 39853 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 54.4 187.7 374.8 58.3 189.9 440.8 76.2 199.1 449.7 240.0 Excluded by Method 4.53 1 0.0003003 1.704e-05 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVSsDEEF.[-] N-Term(TMT6plex); S9(Phospho) 1 P16521 P16521 0 2 0.0255 0 1 1 885.37192 1769.73657 1769.73562 0.53 0.00047 1032969.0625 CID (Collision Induced Dissociation) MS2 0.0 50 108.4 60.0 118.3412 39540 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 18.7 81.8 174.0 37.6 89.7 206.7 38.4 99.5 216.2 121.0 Excluded by Method 3.92 1 0.0003003 1.613e-05 False High Sequest HT (A2) Unambiguous TPSADSLPLSNSSTDLTISDNIK [K].tPSADSLPLSNsSTDLTISDNIk.[E] N-Term(TMT6plex); S12(Phospho); K23(TMT6plex) 1 P32525 P32525 0 3 0.0279 0 1 1 972.16142 2914.4697 2914.46985 -0.05 -5e-05 1878856.75 CID (Collision Induced Dissociation) MS2 11.43009 60 36.5 50.413 118.3008 39525 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 9.9 36.0 57.5 12.9 31.5 66.7 15.8 28.4 69.1 37.6 Excluded by Method 3.58 1 0.0003003 6.328e-05 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVSsDEEF.[-] N-Term(TMT6plex); S9(Phospho) 1 P16521 P16521 0 2 0.0131 0 1 1 885.37217 1769.73705 1769.73562 0.81 0.00072 747326.8125 CID (Collision Induced Dissociation) MS2 40.63754 50 73.0 60.0 122.0523 40903 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 20.1 67.2 110.9 26.4 52.8 141.2 25.5 62.2 138.7 85.3 Excluded by Method 3.83 1 0.0003003 0.0002322 False High Sequest HT (A2) Unambiguous LFQLGPPSPVK [K].lFQLGPPsPVk.[M] N-Term(TMT6plex); S8(Phospho); K11(TMT6plex) 3 O95997 O95997; Q9NZH5; Q9NZH5-2 0 3 0.356 0 1 1 574.33181 1720.98088 1720.98032 0.33 0.00019 3745204.25 CID (Collision Induced Dissociation) MS2 5.363523 50 198.1 38.279 122.0567 40904 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 217.5 206.9 175.2 202.5 185.9 188.5 237.4 207.5 198.0 161.8 Excluded by Method 3.68 1 0.0003003 2.094e-05 False High Sequest HT (A2) Unambiguous SSSPSASLTENELLWEPTPVK [K].ssSPSASLTENELLWEPTPVk.[L] N-Term(TMT6plex); S2(Phospho); K21(TMT6plex) 1 O96028-3 O96028-3 0 3 0.0027 0 1 1 937.47799 2810.41942 2810.42653 -2.53 -0.00237 673023.9375 CID (Collision Induced Dissociation) MS2 59.72027 30 48.4 60.0 126.6504 42594 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 57.8 49.7 36.9 49.1 48.7 39.0 56.1 52.4 51.1 43.1 Excluded by Method 3.76 1 0.0003003 0.0004989 False High Sequest HT (A2) Unambiguous LAAVPAAGPASAGGAAAASGDAAAEEEKEEEAAEESDDDMGFGLFD [K].lAAVPAAGPASAGGAAAASGDAAAEEEkEEEAAEEsDDDmGFGLFD.[-] N-Term(TMT6plex); K28(TMT6plex); S36(Phospho); M40(Oxidation) 1 P05319 P05319 1 4 0.4426 0 1 1 1231.30572 4922.20106 4922.17633 5.02 0.00618 1288259.375 CID (Collision Induced Dissociation) MS2 0.0 30 30.8 60.0 126.4556 42521 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 8.5 22.5 45.6 11.5 22.4 51.9 15.2 30.5 65.0 35.1 Excluded by Method 6.19 1 0.0003003 1.62e-06 False High Sequest HT (A2) Unambiguous DLVLPTQALPASPALK [K].dLVLPTQALPAsPALk.[N] N-Term(TMT6plex); S12(Phospho); K16(TMT6plex) 1 Q15554 Q15554 0 3 0.5151 0 1 1 724.75294 2172.24425 2172.24453 -0.13 -9e-05 4094416.0 CID (Collision Induced Dissociation) MS2 1.010054 40 148.6 27.342 126.3141 42470 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 156.7 136.4 116.1 165.3 142.5 144.2 172.2 153.8 153.2 145.4 Excluded by Method 5.3 1 0.0003003 0.000116 False High Sequest HT (A2) Unambiguous FLYGSDSELALPSSK [K].fLYGsDSELALPSSk.[R] N-Term(TMT6plex); S5(Phospho); K15(TMT6plex) 1 P45976 P45976 0 3 0.0107 0 1 1 718.03773 2152.09865 2152.09792 0.34 0.00024 592048.5 CID (Collision Induced Dissociation) MS2 0.0 50 91.0 60.0 126.1485 42409 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 36.9 64.2 142.0 36.6 75.5 163.4 42.5 95.6 162.5 91.1 Excluded by Method 3.73 1 0.0003003 4.742e-05 False High Sequest HT (A2) Unambiguous APLATGEDDDDEVPDLVENFDEASK [K].aPLATGEDDDDEVPDLVENFDEASk.[N] N-Term(TMT6plex); K25(TMT6plex) 2 P20290 P20290-2; P20290 0 3 0.5712 0 1 1 1050.50592 3149.5032 3149.50503 -0.58 -0.00061 1488320.125 CID (Collision Induced Dissociation) MS2 50.18258 40 72.0 40.442 126.784 42645 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 80.7 69.2 58.7 74.5 64.6 60.9 84.4 74.1 77.6 75.7 Excluded by Method 6.39 1 0.0003003 9.894e-09 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVssDEEF.[-] N-Term(TMT6plex); S8(Phospho); S9(Phospho) 1 P16521 P16521 0 2 0.1148 0 1 1 925.35459 1849.70191 1849.70195 -0.02 -2e-05 7003290.0 CID (Collision Induced Dissociation) MS2 3.804511 40 129.8 26.437 125.8968 42314 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 35.4 90.8 216.4 32.3 103.0 254.9 38.3 126.0 262.9 138.0 Excluded by Method 4.79 1 0.0003003 3.478e-05 False High Sequest HT (A2) Unambiguous LSLGQYDNDAGGQLPFSK [K].lsLGQYDNDAGGQLPFSk.[C] N-Term(TMT6plex); S2(Phospho); K18(TMT6plex) 2 Q68CZ2 Q68CZ2; Q68CZ2-2 0 3 0.0119 0 1 1 816.74585 2448.22301 2448.22123 0.73 0.00059 1154032.625 CID (Collision Induced Dissociation) MS2 5.477354 50 137.1 54.714 125.6597 42227 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 143.2 134.0 128.7 131.8 124.5 134.4 155.1 141.5 141.9 135.9 Excluded by Method 5.04 1 0.0003003 8.165e-08 False High Sequest HT (A2) Unambiguous EEVTEGQAEATEWAEENADNVEW [K].eEVTEGQAEATEWAEENADNVEW.[-] N-Term(TMT6plex) 1 P46654 P46654 0 3 0.5784 0 1 1 955.75653 2865.25504 2865.25362 0.5 0.00047 557245.5 CID (Collision Induced Dissociation) MS2 0.0 50 57.4 60.0 125.0248 41995 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 11.4 47.1 90.6 19.6 47.1 111.7 14.4 48.6 121.2 62.1 Excluded by Method 4.91 1 0.0003003 1.791e-05 False High Sequest HT (A2) Unambiguous GYALVPIIVNSSTPK [K].gYALVPIIVNSStPk.[S] N-Term(TMT6plex); T13(Phospho); K15(TMT6plex) 2 P82094 P82094; P82094-2 0 3 0.0159 0 1 1 699.73148 2097.1799 2097.17611 1.8 0.00126 587624.625 CID (Collision Induced Dissociation) MS2 9.618839 70 41.5 60.0 125.9176 42322 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 41.4 36.1 33.2 43.9 38.3 47.2 45.1 43.3 42.6 44.2 3.78 1 0.0003003 0.0003624 False High Sequest HT (A2) Unambiguous FLYGSDSELALPSSK [K].fLYGsDSELALPSSk.[R] N-Term(TMT6plex); S5(Phospho); K15(TMT6plex) 1 P45976 P45976 0 3 0.0101 0 1 1 718.03731 2152.09737 2152.09792 -0.26 -0.00019 1183474.875 CID (Collision Induced Dissociation) MS2 6.403069 50 187.6 60.0 125.0127 41990 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 47.8 151.5 335.4 57.3 147.5 352.7 62.7 165.0 366.3 190.0 Excluded by Method 3.97 1 0.0003003 4.821e-06 False High Sequest HT (A2) Unambiguous FTPVNITSPNLSFQK [K].fTPVNITsPNLSFQk.[R] N-Term(TMT6plex); S8(Phospho); K15(TMT6plex) 1 Q06685 Q06685 0 3 0.1181 0 1 1 744.40177 2231.19077 2231.18774 1.36 0.00101 2652502.5 CID (Collision Induced Dissociation) MS2 0.0 40 49.5 29.012 126.8816 42683 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 15.6 34.2 79.0 15.9 41.3 101.9 18.5 47.3 91.2 49.8 Excluded by Method 3.81 1 0.0003003 0.0004931 False High Sequest HT (A2) Unambiguous LGDVSPTQIDVSQFGSFK [K].lGDVsPTQIDVSQFGSFk.[E] N-Term(TMT6plex); S5(Phospho); K18(TMT6plex) 1 P46821 P46821 0 3 0.0084 0 1 1 821.75714 2463.25686 2463.25728 -0.17 -0.00014 2551553.5 CID (Collision Induced Dissociation) MS2 19.63327 50 10.0 15.448 128.3066 43306 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 13.0 10.9 8.1 8.8 12.8 11.5 12.5 7.2 7.4 8.0 Excluded by Method 4.74 1 0.0003003 3.628e-06 False High Sequest HT (A2) Unambiguous EEEAAEESDDDMGFGLFD [K].eEEAAEEsDDDmGFGLFD.[-] N-Term(TMT6plex); S8(Phospho); M12(Oxidation) 2 P05319 P10622; P05319 0 2 0.8149 0 1 1 1165.94631 2330.88535 2330.87332 5.16 0.00602 6727555.5 CID (Collision Induced Dissociation) MS2 21.26449 70 36.4 9.794 127.8861 43110 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 9.2 32.4 60.0 12.9 32.0 66.7 10.0 35.3 76.7 29.1 4.43 1 0.0003003 0.000539 False High Sequest HT (A2) Unambiguous AILESVGIEIEDEK [K].aILESVGIEIEDEk.[V] N-Term(TMT6plex); K14(TMT6plex) 1 P05319 P05319 0 3 0.3432 0 1 1 668.38137 2003.12955 2003.13126 -0.85 -0.00057 909927.0 CID (Collision Induced Dissociation) MS2 43.60561 50 108.8 60.0 126.9288 42701 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 35.5 100.0 153.9 45.3 89.7 207.3 40.5 98.3 208.1 109.6 Excluded by Method 3.38 1 0.0003003 2.58e-05 False High Sequest HT (A2) Unambiguous ELVGPPLAETVFTPK [K].eLVGPPLAETVFtPk.[T] N-Term(TMT6plex); T13(Phospho); K15(TMT6plex) 1 P49792 P49792 0 3 0.2542 0 1 1 712.72988 2136.17509 2136.17578 -0.32 -0.00023 5823727.5 CID (Collision Induced Dissociation) MS2 15.9119 30 95.1 15.667 127.2867 42842 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 96.3 89.0 71.2 98.0 90.0 107.7 109.9 91.3 100.7 96.8 Excluded by Method 2.4 1 0.0003003 0.0003112 False High Sequest HT (A2) Unambiguous VQGEAVSNIQENTQTPTVQEESEEEEVDETGVEVK [K].vQGEAVSNIQENTQTPTVQEEsEEEEVDETGVEVk.[D] N-Term(TMT6plex); S22(Phospho); K35(TMT6plex) 3 Q13765 Q13765; E9PAV3; E9PAV3-2 0 4 0.0145 0 1 1 1100.52099 4399.06211 4399.06016 0.44 0.00049 850079.75 CID (Collision Induced Dissociation) MS2 10.20485 50 21.7 46.872 127.243 42825 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 21.1 23.2 13.0 22.7 18.0 22.1 21.9 28.0 24.2 22.8 Excluded by Method 4.13 1 0.0003003 0.0003205 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVssDEEF.[-] N-Term(TMT6plex); S8(Phospho); S9(Phospho) 1 P16521 P16521 0 2 0.1371 0 1 1 925.35466 1849.70203 1849.70195 0.04 4e-05 6191965.5 CID (Collision Induced Dissociation) MS2 9.044225 50 116.8 33.546 127.0133 42733 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 25.0 96.3 194.9 32.7 97.3 235.1 33.5 106.7 228.0 118.4 Excluded by Method 4.23 1 0.0003003 0.0001264 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVssDEEF.[-] N-Term(TMT6plex); S8(Phospho); S9(Phospho) 1 P16521 P16521 0 2 0.1294 0 1 1 925.35453 1849.70179 1849.70195 -0.09 -8e-05 6990656.5 CID (Collision Induced Dissociation) MS2 7.658944 40 192.1 23.38 124.8065 41913 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 50.9 154.9 318.1 51.6 144.7 372.8 63.5 164.0 386.3 214.5 Excluded by Method 4.56 1 0.0003003 3.777e-05 False High Sequest HT (A2) Unambiguous FVQGLLQNAAANAEAK [K].fVQGLLQNAAANAEAk.[G] N-Term(TMT6plex); K16(TMT6plex) 2 P46990 P46990; P05740 0 3 0.413 0 1 1 701.73716 2103.19692 2103.19626 0.31 0.00022 1028746.5625 CID (Collision Induced Dissociation) MS2 16.8982 60 119.7 60.0 122.9752 41236 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 31.9 104.1 169.7 44.5 97.0 237.2 48.4 114.8 229.9 119.8 Excluded by Method 4.31 1 0.0003003 3.574e-06 False High Sequest HT (A2) Unambiguous GVWGTPGQVYDVEEVDVK [K].gVWGtPGQVYDVEEVDVk.[D] N-Term(TMT6plex); T5(Phospho); K18(TMT6plex) 2 Q53EL6 Q53EL6-2; Q53EL6 0 3 0.1412 0 1 1 839.08988 2515.25508 2515.25219 1.15 0.00096 441469.03125 CID (Collision Induced Dissociation) MS2 10.79496 60 24.9 60.0 123.2883 41351 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 21.0 23.7 22.0 28.5 21.5 29.7 24.8 29.5 21.8 26.9 Excluded by Method 4.25 1 0.0003003 1.453e-05 False High Sequest HT (A2) Unambiguous DITDPLSLNTCTDEGHVVLASPLK [K].dITDPLSLNTcTDEGHVVLAsPLk.[T] N-Term(TMT6plex); C11(Carbamidomethyl); S21(Phospho); K24(TMT6plex) 1 Q7L2J0 Q7L2J0 0 4 0.3194 0 1 1 784.15203 3133.58627 3133.58925 -0.95 -0.00075 1792586.875 CID (Collision Induced Dissociation) MS2 5.525775 10 33.6 60.0 122.3378 41010 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 37.7 34.4 29.2 38.2 31.3 31.8 32.9 33.4 33.7 33.2 Excluded by Method 7.64 1 0.0003003 1.063e-05 False High Sequest HT (A2) Unambiguous VPTVGSYGVAGATLPETIPTSK [K].vPTVGsYGVAGATLPETIPTSk.[N] N-Term(TMT6plex); S6(Phospho); K22(TMT6plex) 1 P38250 P38250 0 4 0.0157 0 1 1 671.61421 2683.43502 2683.43597 -0.35 -0.00024 1108746.625 CID (Collision Induced Dissociation) MS2 13.13341 30 76.5 60.0 122.1637 40944 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 25.4 59.4 127.6 23.8 63.4 148.6 24.8 61.1 158.0 72.7 Excluded by Method 5.08 1 0.0003003 3.374e-05 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVssDEEF.[-] N-Term(TMT6plex); S8(Phospho); S9(Phospho) 1 P16521 P16521 0 2 0.1231 0 1 1 925.35453 1849.70179 1849.70195 -0.09 -8e-05 7246736.0 CID (Collision Induced Dissociation) MS2 1.888065 40 171.3 38.172 122.5049 41072 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 38.5 144.5 269.5 46.0 135.0 358.1 51.9 154.3 340.6 174.2 Excluded by Method 4.71 1 0.0003003 1.884e-05 False High Sequest HT (A2) Unambiguous GSGEDDEEETETTPTSTVPVATIAQETLK [K].gSGEDDEEETETtPTSTVPVATIAQETLk.[V] N-Term(TMT6plex); T13(Phospho); K29(TMT6plex) 1 P12962 P12962 0 3 0.036 0 1 1 1191.90355 3573.69611 3573.69846 -0.66 -0.00079 655625.8125 CID (Collision Induced Dissociation) MS2 0.0 40 72.0 60.0 123.4441 41408 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 16.2 58.7 120.2 18.8 60.9 130.9 24.3 70.0 139.2 81.0 Excluded by Method 5.56 1 0.0003003 7.735e-07 False High Sequest HT (A2) Unambiguous ITEIEDGQDGGVTPYSNESGLVNADLDVK [K].iTEIEDGQDGGVtPYSNESGLVNADLDVk.[D] N-Term(TMT6plex); T13(Phospho); K29(TMT6plex) 1 P04050 P04050 0 4 0.0778 0 1 1 894.18526 3573.71923 3573.72495 -1.6 -0.00143 734286.5625 CID (Collision Induced Dissociation) MS2 0.0 30 27.3 46.25 124.6235 41844 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 3.6 22.5 36.9 8.2 23.4 52.3 11.4 28.6 59.2 26.7 Excluded by Method 7.58 1 0.0003003 1.621e-05 False High Sequest HT (A2) Unambiguous YAAAAPAATSAASGDAAPAEEAAAEEEEESDDDMGFGLFD [K].yAAAAPAATSAASGDAAPAEEAAAEEEEEsDDDmGFGLFD.[-] N-Term(TMT6plex); S30(Phospho); M34(Oxidation) 1 P05317 P05317 0 4 0.2073 0 1 1 1062.19548 4245.76007 4245.73286 6.41 0.0068 797075.3125 CID (Collision Induced Dissociation) MS2 23.93638 30 5.4 60.0 124.2265 41698 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 2.4 2.1 12.4 3.6 13.1 3.1 11.0 6.6 Excluded by Method 4.63 1 0.0003003 0.0003326 False High Sequest HT (A2) Unambiguous LALSPLSLGK [K].lALsPLSLGk.[E] N-Term(TMT6plex); S4(Phospho); K10(TMT6plex) 1 P49454 P49454 0 2 0.0981 0 1 1 768.9621 1536.91693 1536.91665 0.18 0.00014 3907786.25 CID (Collision Induced Dissociation) MS2 5.018425 40 104.1 41.627 123.6782 41494 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 99.9 101.9 87.0 108.3 100.4 101.9 112.6 111.7 106.4 110.9 Excluded by Method 3.67 1 0.0003003 0.0004883 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVssDEEF.[-] N-Term(TMT6plex); S8(Phospho); S9(Phospho) 1 P16521 P16521 0 2 0.133 0 1 1 925.35447 1849.70167 1849.70195 -0.15 -0.00014 6496939.5 CID (Collision Induced Dissociation) MS2 0.0 50 212.4 27.19 123.6605 41487 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 53.5 158.6 343.1 64.5 163.3 457.9 74.5 179.1 430.1 199.4 Excluded by Method 4.51 1 0.0003003 0.0003158 False High Sequest HT (A2) Unambiguous SFLVIVDGSPK [K].sFLVIVDGsPk.[N] N-Term(TMT6plex); S9(Phospho); K11(TMT6plex) 2 Q9H116 Q9H116; Q9H116-4 0 3 0.6005 0 1 1 567.31882 1699.94191 1699.9436 -0.99 -0.00056 543081.0 CID (Collision Induced Dissociation) MS2 32.32129 70 48.8 60.0 123.6082 41468 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 45.4 46.5 40.0 56.9 39.7 52.4 53.5 50.2 52.8 50.8 4.13 1 0.0003003 0.0001315 False High Sequest HT (A2) Unambiguous EYVPLDNAEQSTSSSQETK [K].eYVPLDNAEQSTSssQETk.[E] N-Term(TMT6plex); S14(Phospho); S15(Phospho); K19(TMT6plex) 1 P33322 P33322 0 3 0.0667 0 1 1 911.07672 2731.21561 2731.21529 0.12 0.00011 235452.46875 CID (Collision Induced Dissociation) MS2 0.0 40 38.2 60.0 89.0101 28572 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 16.1 36.3 46.6 17.0 35.8 57.4 16.9 37.1 75.3 43.9 Excluded by Method 3.3 1 0.0003003 0.000283 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVSsDEEF.[-] N-Term(TMT6plex); S9(Phospho) 1 P16521 P16521 0 2 0.0439 0 1 1 885.37149 1769.73571 1769.73562 0.05 4e-05 34224124.0 CID (Collision Induced Dissociation) MS2 0.0 60 233.5 11.369 111.1519 36882 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 54.1 196.2 393.8 61.9 185.9 448.5 75.8 200.9 481.8 236.5 Excluded by Method 4.56 1 0.0003003 9.869e-07 False High Sequest HT (A2) Unambiguous ESQIDVLQQDELDSYPDSVK [K].eSQIDVLQQDELDSyPDSVk.[K] N-Term(TMT6plex); Y15(Phospho); K20(TMT6plex) 1 Q9NQS3 Q9NQS3 0 3 0.0088 0 1 1 949.46333 2846.37543 2846.37489 0.19 0.00018 450005.9375 CID (Collision Induced Dissociation) MS2 9.892038 50 32.4 60.0 111.0419 36842 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 33.4 24.6 29.3 30.6 32.6 39.7 30.0 36.4 35.9 31.8 Excluded by Method 4.55 1 0.0003003 3.028e-05 False High Sequest HT (A2) Unambiguous FLNINRVCSSDSDLPGSDIK [K].fLNINRVcsSDSDLPGSDIk.[S] N-Term(TMT6plex); C8(Carbamidomethyl); S9(Phospho); K20(TMT6plex) 1 P27466 P27466 1 3 0.1395 0 1 1 925.79685 2775.37601 2775.37887 -1.03 -0.00095 999386.4375 CID (Collision Induced Dissociation) MS2 17.59788 50 90.8 60.0 111.87 37151 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 27.7 73.0 141.4 34.2 77.3 158.4 36.7 103.0 168.7 87.4 Excluded by Method 3.37 1 0.0003003 0.000373 False High Sequest HT (A2) Unambiguous VFVGGLSPDTSEEQIK [K].vFVGGLsPDTSEEQIk.[E] N-Term(TMT6plex); S7(Phospho); K16(TMT6plex) 3 O14979 O14979-3; O14979; O14979-2 0 3 0.108 0 1 1 748.72458 2244.15919 2244.1565 1.2 0.0009 1109343.0 CID (Collision Induced Dissociation) MS2 0.0 40 105.5 60.0 112.4525 37369 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 106.1 103.8 90.8 101.1 97.4 111.6 127.8 105.4 104.4 106.4 Excluded by Method 5.37 1 0.0003003 3.895e-05 False High Sequest HT (A2) Unambiguous DNLSDTEEVIVIK [K].dNLsDTEEVIVIk.[K] N-Term(TMT6plex); S4(Phospho); K13(TMT6plex) 1 Q08951 Q08951 0 3 0.0055 0 1 1 671.69014 2013.05587 2013.05572 0.07 5e-05 4229273.0 CID (Collision Induced Dissociation) MS2 7.012022 60 173.6 29.322 112.424 37357 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 48.0 141.6 249.9 55.5 143.3 333.7 58.4 171.8 354.2 179.3 Excluded by Method 3.63 1 0.0003003 0.0003714 False High Sequest HT (A2) Unambiguous VQGEAVSNIQENTQTPTVQEESEEEEVDETGVEVK [K].vQGEAVSNIQENTQTPTVQEEsEEEEVDETGVEVk.[D] N-Term(TMT6plex); S22(Phospho); K35(TMT6plex) 3 Q13765 Q13765; E9PAV3; E9PAV3-2 0 4 0.0042 0 1 1 1100.51232 4399.02745 4399.06016 -7.44 -0.00818 713864.375 CID (Collision Induced Dissociation) MS2 31.6118 50 22.8 60.0 112.3717 37337 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 27.0 18.2 17.6 25.0 21.3 24.5 23.8 24.3 20.3 26.3 Excluded by Method 4.79 1 0.0003003 1.102e-05 False High Sequest HT (A2) Unambiguous VVVAENFDEIVNNENK [K].vVVAENFDEIVNNENk.[D] N-Term(TMT6plex); K16(TMT6plex) 1 P30101 P30101 0 3 0.3748 0 1 1 764.41517 2291.23097 2291.22835 1.14 0.00087 3515193.25 CID (Collision Induced Dissociation) MS2 1.578555 50 97.9 60.0 112.1551 37257 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 111.6 87.3 79.8 98.3 91.3 97.8 103.4 105.1 99.7 104.2 Excluded by Method 5.23 1 0.0003003 0.0001407 False High Sequest HT (A2) Unambiguous LTFDTTFSPNTGK [K].lTFDTTFsPNTGk.[K] N-Term(TMT6plex); S8(Phospho); K13(TMT6plex) 3 P45880 P45880; P45880-2; P45880-1 0 2 0.0783 0 1 1 984.001 1966.99472 1966.99273 1.01 0.001 467514.6875 CID (Collision Induced Dissociation) MS2 0.0 50 109.2 60.0 112.0281 37209 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 111.5 118.4 82.8 101.8 103.5 108.5 121.4 109.4 118.0 117.1 Excluded by Method 5.11 1 0.0003003 3.674e-05 False High Sequest HT (A2) Unambiguous NYWDVNDSPIIK [K].nYWDVNDsPIIk.[V] N-Term(TMT6plex); S8(Phospho); K12(TMT6plex) 1 P23201 P23201 0 2 0.4659 0 1 1 1001.50653 2002.00579 2002.00872 -1.46 -0.00146 580334.5 CID (Collision Induced Dissociation) MS2 0.0 60 107.4 60.0 111.9989 37198 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 20.2 87.5 179.6 35.7 85.3 201.8 41.9 100.5 227.9 93.7 Excluded by Method 5.28 1 0.0003003 3.226e-07 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVSsDEEF.[-] N-Term(TMT6plex); S9(Phospho) 1 P16521 P16521 0 2 0.0455 0 1 1 885.37168 1769.73608 1769.73562 0.26 0.00023 14131541.0 CID (Collision Induced Dissociation) MS2 0.0 40 155.3 17.868 112.284 37305 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 34.9 118.4 230.7 38.9 122.7 319.6 43.9 146.8 326.1 170.9 Excluded by Method 4.4 1 0.0003003 1.748e-05 False High Sequest HT (A2) Unambiguous QGSPDQVSPVSEMTSTSLYQDK [K].qGsPDQVSPVSEMTSTSLYQDk.[Q] N-Term(TMT6plex); S3(Phospho); K22(TMT6plex) 1 P46821 P46821 0 3 0.0825 0 1 1 974.7999 2922.38515 2922.38441 0.26 0.00025 888336.0625 CID (Collision Induced Dissociation) MS2 34.24566 70 26.3 60.0 108.9428 36055 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 25.6 28.3 20.3 27.0 23.6 28.8 25.8 26.1 29.9 27.7 4.12 1 0.0003003 2.054e-05 False High Sequest HT (A2) Unambiguous TLSVAAAFNEDEDSEPEEMPPEAK [K].tLSVAAAFNEDEDsEPEEmPPEAk.[M] N-Term(TMT6plex); S14(Phospho); M19(Oxidation); K24(TMT6plex) 1 Q8WW12 Q8WW12 0 3 0.2797 0 1 1 1054.1491 3160.43275 3160.43214 0.19 0.0002 659100.375 CID (Collision Induced Dissociation) MS2 18.90506 20 18.8 60.0 108.5124 35898 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 16.2 16.3 19.9 13.4 20.1 16.8 22.4 21.2 20.5 21.3 Excluded by Method 3.54 1 0.0003003 2.122e-05 False High Sequest HT (A2) Unambiguous DFDLENDEYELSEEEK [K].dFDLENDEYELsEEEk.[N] N-Term(TMT6plex); S12(Phospho); K16(TMT6plex) 1 Q03661 Q03661 0 3 0.4067 0 1 1 848.04354 2542.11607 2542.11621 -0.06 -5e-05 720845.125 CID (Collision Induced Dissociation) MS2 30.73294 40 61.8 60.0 108.4917 35890 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 17.6 50.2 84.3 24.1 55.2 108.9 27.9 65.0 124.1 60.4 Excluded by Method 5.63 1 0.0003003 4.593e-06 False High Sequest HT (A2) Unambiguous AYEELSNTDLEFK [K].aYEELsNTDLEFk.[F] N-Term(TMT6plex); S6(Phospho); K13(TMT6plex) 1 P16521 P16521 0 3 0.0759 0 1 1 699.67796 2097.01932 2097.01934 -0.01 -1e-05 3275233.0 CID (Collision Induced Dissociation) MS2 3.108511 30 114.2 28.845 108.2434 35799 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 27.0 82.4 182.9 37.3 88.4 215.3 41.0 103.1 242.9 121.4 Excluded by Method 3.95 1 0.0003003 0.000247 False High Sequest HT (A2) Unambiguous DMSPLSETEMALGK [K].dmsPLSETEmALGk.[D] N-Term(TMT6plex); M2(Oxidation); S3(Phospho); M10(Oxidation); K14(TMT6plex) 3 P27816 P27816; P27816-6; P27816-2 0 3 0.1041 0 1 1 693.66393 2078.97723 2078.97913 -0.92 -0.00064 837404.125 CID (Collision Induced Dissociation) MS2 28.61522 50 50.0 60.0 110.4704 36629 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 46.5 54.4 43.9 53.4 47.6 56.5 46.3 44.9 55.8 50.2 Excluded by Method 2.69 1 0.0003003 0.0005401 False High Sequest HT (A2) Unambiguous APEGELGDSLQTAFDEGK [K].aPEGELGDSLQTAFDEGk.[D] N-Term(TMT6plex); K18(TMT6plex) 1 P23301 P23301 0 3 0.605 0 1 1 774.73474 2322.18967 2322.18654 1.35 0.00104 2975284.25 CID (Collision Induced Dissociation) MS2 0.0 80 101.6 46.9 110.3589 36586 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 26.3 82.7 155.7 32.5 74.1 192.5 39.1 91.3 217.5 104.5 6.05 1 0.0003003 1.308e-07 False High Sequest HT (A2) Unambiguous AGLSPAEPLLSRAGFSPDSSK [K].aGLSPAEPLLSRAGFsPDSSk.[A] N-Term(TMT6plex); S16(Phospho); K21(TMT6plex) 1 O95402 O95402 1 3 0.1962 0 1 1 875.79485 2625.36999 2625.36895 0.39 0.00034 973280.375 CID (Collision Induced Dissociation) MS2 10.08654 40 61.8 60.0 109.636 36317 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 55.4 56.1 45.9 72.9 61.6 68.2 71.5 67.1 62.6 56.7 Excluded by Method 3.72 1 0.0003003 0.000159 False High Sequest HT (A2) Unambiguous DNGFDQLPVLTEDGK [K].dNGFDQLPVLTEDGk.[L] N-Term(TMT6plex); K15(TMT6plex) 1 P32582 P32582 0 3 0.4191 0 1 1 702.70859 2106.11122 2106.11192 -0.33 -0.00023 691073.8125 CID (Collision Induced Dissociation) MS2 0.0 50 104.5 60.0 110.2561 36547 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 23.9 83.8 160.1 34.4 89.9 196.7 42.8 99.7 201.1 112.6 Excluded by Method 4.51 1 0.0003003 7.817e-05 False High Sequest HT (A2) Unambiguous STELLLGVK [K].sTELLLGVk.[T] N-Term(TMT6plex); S1(Phospho); K9(TMT6plex) 2 Q6R327 Q6R327; Q6R327-3 0 2 0.0864 0 1 1 749.43869 1497.87011 1497.86937 0.49 0.00037 1585213.0 CID (Collision Induced Dissociation) MS2 0.0 60 117.4 60.0 110.2427 36542 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 112.0 111.5 85.4 126.4 108.1 126.1 136.6 132.8 117.1 117.8 Excluded by Method 4.05 1 0.0003003 0.0002594 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVsSDEEF.[-] N-Term(TMT6plex); S8(Phospho) 1 P16521 P16521 0 2 0.0387 0 1 1 885.37168 1769.73608 1769.73562 0.26 0.00023 3094355.25 CID (Collision Induced Dissociation) MS2 12.4442 40 128.6 51.023 109.9583 36434 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 33.6 103.4 206.9 37.6 112.5 238.0 41.1 133.8 250.0 129.5 Excluded by Method 3.88 1 0.0003003 0.0002427 False High Sequest HT (A2) Unambiguous EVDPSTGELQSLQMPESEGPSSLDPSQEGPTGLK [K].eVDPSTGELQSLQmPESEGPSsLDPSQEGPTGLk.[E] N-Term(TMT6plex); M14(Oxidation); S22(Phospho); K34(TMT6plex) 2 Q13501 Q13501-2; Q13501 0 4 0.0057 0 1 1 1020.98209 4080.90653 4080.92485 -4.49 -0.00458 882877.75 CID (Collision Induced Dissociation) MS2 0.0 40 41.8 60.0 109.9465 36429 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 39.8 39.7 38.9 43.5 39.9 43.8 43.9 45.9 41.6 40.7 Excluded by Method 7.03 1 0.0003003 1.834e-06 False High Sequest HT (A2) Unambiguous SSENVQVDLANFEK [K].ssENVQVDLANFEk.[A] N-Term(TMT6plex); S2(Phospho); K14(TMT6plex) 1 P22147 P22147 0 3 0.0102 0 1 1 706.68832 2118.05042 2118.05204 -0.77 -0.00054 1959455.25 CID (Collision Induced Dissociation) MS2 0.0 50 169.8 52.052 110.2832 36557 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 40.1 137.1 270.2 48.3 146.4 316.4 59.8 155.3 337.9 186.2 Excluded by Method 4.89 1 0.0003003 2.447e-06 False High Sequest HT (A2) Unambiguous VTFSVSPIEATEK [K].vTFSVsPIEATEk.[C] N-Term(TMT6plex); S6(Phospho); K13(TMT6plex) 3 Q9ULD2 Q9ULD2-5; Q9ULD2; Q9ULD2-2 0 3 0.0646 0 1 1 649.34764 1946.02837 1946.02878 -0.21 -0.00014 576401.875 CID (Collision Induced Dissociation) MS2 0.0 60 57.1 60.0 112.6466 37441 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 50.9 58.7 54.7 49.6 51.3 68.0 54.3 66.1 62.6 55.3 Excluded by Method 3.56 1 0.0003003 0.0002718 False High Sequest HT (A2) Unambiguous EETDTDLEVVLEK [K].eEtDTDLEVVLEk.[K] N-Term(TMT6plex); T3(Phospho); K13(TMT6plex) 1 Q1ED39 Q1ED39 0 3 0.0028 0 1 1 686.68158 2058.03017 2058.02957 0.29 0.0002 2442845.5 CID (Collision Induced Dissociation) MS2 3.613065 60 167.9 60.0 115.5594 38512 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 169.9 167.4 138.5 166.1 153.1 170.8 196.4 171.9 167.4 177.1 Excluded by Method 3.51 1 0.0003003 0.0001759 False High Sequest HT (A2) Unambiguous NPHDIIEGINAGEIEIPEN [K].nPHDIIEGINAGEIEIPEN.[-] N-Term(TMT6plex) 1 P0CX53 P0CX53 0 3 0.3011 0 1 1 768.39552 2303.17199 2303.17167 0.14 0.00011 4726475.0 CID (Collision Induced Dissociation) MS2 1.658129 10 109.3 23.399 115.4381 38468 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 24.7 87.2 177.2 25.0 75.0 234.9 39.7 92.6 222.5 114.7 Excluded by Method 2.69 1 0.0003003 0.0002467 False High Sequest HT (A2) Unambiguous LPWEVSDGEVSSGK [K].lPWEVsDGEVSSGk.[T] N-Term(TMT6plex); S6(Phospho); K14(TMT6plex) 1 P48415 P48415 0 3 0.5118 0 1 1 676.67494 2028.01025 2028.00911 0.56 0.00038 1088879.625 CID (Collision Induced Dissociation) MS2 2.866628 50 188.2 60.0 114.8195 38244 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 53.1 154.4 301.5 56.1 165.6 361.0 61.2 162.4 370.9 196.2 Excluded by Method 5.1 1 0.0003003 1.735e-05 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVssDEEF.[-] N-Term(TMT6plex); S8(Phospho); S9(Phospho) 1 P16521 P16521 0 2 0.1245 0 1 1 925.35411 1849.70094 1849.70195 -0.55 -0.00051 2591257.75 CID (Collision Induced Dissociation) MS2 0.0 40 124.6 60.0 115.8092 38600 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 29.2 94.5 217.6 38.2 96.1 250.2 37.4 108.9 237.1 136.9 Excluded by Method 4.58 1 0.0003003 0.0002183 False High Sequest HT (A2) Unambiguous LFPGSPAIYK [K].lFPGsPAIYk.[L] N-Term(TMT6plex); S5(Phospho); K10(TMT6plex) 9 P49792 P0DJD0; P49792; Q99666; Q7Z3J3-2; P0DJD1; Q99666-2; O14715; A6NKT7; Q7Z3J3 0 2 0.2198 0 1 1 815.95368 1630.90008 1630.901 -0.56 -0.00046 706947.8125 CID (Collision Induced Dissociation) MS2 11.94559 30 76.3 60.0 117.1047 39078 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 77.8 74.4 69.4 65.1 74.5 86.2 80.2 79.5 80.7 74.9 Excluded by Method 3.73 1 0.0003003 0.0005258 False High Sequest HT (A2) Unambiguous VVADDLVLVTPK [K].vVADDLVLVtPk.[K] N-Term(TMT6plex); T10(Phospho); K12(TMT6plex) 1 P38934 P38934 0 3 0.2981 0 1 1 603.01793 1807.03924 1807.03822 0.56 0.00034 534708.4375 CID (Collision Induced Dissociation) MS2 34.69899 80 81.8 60.0 116.8252 38974 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 21.7 64.0 123.4 29.1 65.9 159.1 34.4 81.7 165.9 73.1 4.16 1 0.0003003 0.0001555 False High Sequest HT (A2) Unambiguous CSGPGLSPGMVRANLPQSFQVDTSK [K].cSGPGLsPGMVRANLPQSFQVDTSk.[A] N-Term(TMT6plex); C1(Carbamidomethyl); S7(Phospho); K25(TMT6plex) 2 P21333 P21333; P21333-2 1 4 0.0209 0 1 1 793.64902 3171.57426 3171.57323 0.33 0.00026 1386215.75 CID (Collision Induced Dissociation) MS2 3.353931 20 124.7 60.0 116.2675 38769 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 103.3 132.0 114.1 109.0 102.2 144.0 127.5 138.8 159.8 116.7 Excluded by Method 4.31 1 0.0003003 0.0002519 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVSsDEEF.[-] N-Term(TMT6plex); S9(Phospho) 1 P16521 P16521 0 2 0.0695 0 1 1 885.37107 1769.73486 1769.73562 -0.43 -0.00038 5192502.0 CID (Collision Induced Dissociation) MS2 0.0 70 196.5 33.141 113.3784 37712 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 49.8 166.9 326.7 55.9 150.0 373.9 68.7 178.1 391.8 203.4 4.75 1 0.0003003 5.588e-08 False High Sequest HT (A2) Unambiguous MNGRKQSLGELIGTLNAAK [K].mNGRkQsLGELIGTLNAAk.[V] N-Term(TMT6plex); K5(TMT6plex); S7(Phospho); K19(TMT6plex) 2 P60174 P60174; P60174-3 2 4 0.252 0 1 1 692.89238 2768.5477 2768.5461 0.58 0.0004 1170702.125 CID (Collision Induced Dissociation) MS2 3.9864 40 75.0 60.0 113.262 37669 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 81.0 82.1 58.3 70.3 66.6 73.0 81.6 84.7 71.8 80.3 Excluded by Method 3.69 1 0.0003003 5.719e-05 False High Sequest HT (A2) Unambiguous AAADALSDLEIK [K].aAADALsDLEIk.[D] N-Term(TMT6plex); S7(Phospho); K12(TMT6plex) 1 P09938 P09938 0 2 0.5962 0 1 1 877.97069 1754.9341 1754.93415 -0.03 -3e-05 5870386.5 CID (Collision Induced Dissociation) MS2 15.44511 50 154.1 35.447 112.8161 37504 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 36.1 121.5 239.5 49.7 120.4 297.8 46.8 147.1 316.9 165.6 Excluded by Method 5.25 1 0.0003003 2.854e-06 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVssDEEF.[-] N-Term(TMT6plex); S8(Phospho); S9(Phospho) 1 P16521 P16521 0 2 0.1328 0 1 1 925.35472 1849.70216 1849.70195 0.11 0.0001 1471447.75 CID (Collision Induced Dissociation) MS2 0.0 40 69.5 60.0 113.5162 37762 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 21.2 58.2 110.7 18.2 56.2 145.7 23.6 57.9 139.0 64.4 Excluded by Method 3.99 1 0.0003003 0.0004685 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVsSDEEF.[-] N-Term(TMT6plex); S8(Phospho) 1 P16521 P16521 0 2 0.0223 0 1 1 885.37076 1769.73425 1769.73562 -0.78 -0.00069 3110187.75 CID (Collision Induced Dissociation) MS2 3.480417 40 159.5 60.0 114.5513 38143 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 38.1 131.6 247.2 48.7 126.2 330.7 43.3 148.4 314.1 166.7 Excluded by Method 4.94 1 0.0003003 1.351e-06 False High Sequest HT (A2) Unambiguous LIREASPATEFANEFTGENSTAK [K].lIREASPAtEFANEFTGENSTAk.[S] N-Term(TMT6plex); T9(Phospho); K23(TMT6plex) 1 P13259 P13259 1 4 0.0679 0 1 1 756.12954 3021.49633 3021.49707 -0.24 -0.00018 464970.53125 CID (Collision Induced Dissociation) MS2 6.463033 20 32.2 60.0 114.4627 38110 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 11.3 23.0 48.7 11.4 26.1 55.7 15.1 28.1 68.9 33.7 Excluded by Method 3.24 1 0.0003003 9.566e-05 False High Sequest HT (A2) Unambiguous DISASSPNLGGILK [K].dISASsPNLGGILk.[A] N-Term(TMT6plex); S6(Phospho); K14(TMT6plex) 1 Q08548 Q08548 0 3 0.0259 0 1 1 637.35175 1910.04069 1910.04002 0.35 0.00022 1434420.625 CID (Collision Induced Dissociation) MS2 9.584052 40 170.7 60.0 114.2787 38041 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 57.0 147.7 281.8 52.0 141.0 315.2 54.7 167.8 322.2 167.7 Excluded by Method 4.24 1 0.0003003 0.0001671 False High Sequest HT (A2) Unambiguous VTPDIEESLLEPENEK [K].vTPDIEEsLLEPENEk.[I] N-Term(TMT6plex); S8(Phospho); K16(TMT6plex) 1 Q14151 Q14151 0 3 0.0261 0 1 1 794.06961 2380.19429 2380.19367 0.26 0.00021 1529051.375 CID (Collision Induced Dissociation) MS2 53.94995 40 152.9 60.0 114.1393 37992 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 143.5 159.0 117.2 148.2 143.9 152.7 181.4 172.1 151.7 159.1 Excluded by Method 4.59 1 0.0003003 5.943e-06 False High Sequest HT (A2) Unambiguous AYEPDLSDADFEGIEASPK [K].aYEPDLSDADFEGIEAsPk.[L] N-Term(TMT6plex); S17(Phospho); K19(TMT6plex) 1 P49687 P49687 0 3 0.4866 0 1 1 864.74384 2592.21696 2592.21587 0.42 0.00036 374219.90625 CID (Collision Induced Dissociation) MS2 12.79336 50 54.6 60.0 113.9634 37929 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 14.1 43.8 76.9 18.4 44.9 107.3 21.0 49.2 105.8 64.5 Excluded by Method 5.22 1 0.0003003 3.032e-06 False High Sequest HT (A2) Unambiguous VQGEAVSNIQENTQTPTVQEESEEEEVDETGVEVK [K].vQGEAVSNIQENTQTPTVQEEsEEEEVDETGVEVk.[D] N-Term(TMT6plex); S22(Phospho); K35(TMT6plex) 3 Q13765 Q13765; E9PAV3; E9PAV3-2 0 4 0.0322 0 1 1 1100.51977 4399.05723 4399.06016 -0.67 -0.00073 464464.84375 CID (Collision Induced Dissociation) MS2 0.0 40 14.2 60.0 113.6829 37825 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 10.8 14.5 14.0 18.0 7.4 15.2 19.2 12.9 15.0 15.3 Excluded by Method 3.73 1 0.0003003 0.0003316 False High Sequest HT (A2) Unambiguous FSVSSNDSQNSLK [K].fSVSSNDsQNsLk.[N] N-Term(TMT6plex); S8(Phospho); S11(Phospho); K13(TMT6plex) 1 Q06833 Q06833 0 3 0.3325 0 1 1 677.64649 2030.92492 2030.92374 0.58 0.00039 1092348.375 CID (Collision Induced Dissociation) MS2 43.22995 30 72.2 57.39 88.9058 28533 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 23.4 58.1 112.0 30.4 66.5 123.5 31.5 70.5 140.7 65.8 Excluded by Method 3.91 1 0.0003003 7.888e-05 False High Sequest HT (A2) Unambiguous VVDYSQFQESDDADEDYGRDSGPPTK [K].vVDYSQFQEsDDADEDYGRDSGPPTk.[K] N-Term(TMT6plex); S10(Phospho); K26(TMT6plex) 1 Q9H1E3 Q9H1E3 1 4 0.2422 0 1 1 865.38867 3458.53286 3458.53134 0.44 0.00038 893731.5 CID (Collision Induced Dissociation) MS2 3.531206 50 68.2 47.983 88.8759 28521 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 73.1 65.3 56.3 65.0 65.1 74.4 78.3 71.9 68.9 63.5 Excluded by Method 9.25 1 0.0003003 1.868e-10 False High Sequest HT (A2) Unambiguous APVPSTCSSTFPEELSPPSHQAK [K].aPVPSTcSSTFPEELsPPSHQAk.[R] N-Term(TMT6plex); C7(Carbamidomethyl); S16(Phospho); K23(TMT6plex) 5 Q14980 Q14980-3; Q14980-5; Q14980-2; Q14980-4; Q14980 0 3 0.0609 0 1 1 998.15394 2992.44725 2992.45276 -1.84 -0.00184 489378.96875 CID (Collision Induced Dissociation) MS2 0.0 40 23.5 60.0 88.8115 28497 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 28.2 26.3 19.1 20.8 20.7 23.3 26.8 26.2 26.2 17.0 Excluded by Method 6.4 1 0.0003003 6.712e-06 False High Sequest HT (A2) Unambiguous SQENLGNPSK [K].sQENLGNPSk.[N] N-Term(TMT6plex); S1(Phospho); K10(TMT6plex) 2 Q9H0X4 Q9H0X4-2; Q9H0X4 0 2 0.6129 0 1 1 806.41103 1611.81478 1611.81437 0.25 0.0002 739182.4375 CID (Collision Induced Dissociation) MS2 33.3974 40 63.4 60.0 52.1601 14788 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 65.6 61.1 47.9 67.2 68.9 62.0 69.3 67.2 63.5 61.3 Excluded by Method 3.72 1 0.0003003 8.782e-05 False High Sequest HT (A2) Unambiguous SPPAEEAASPEK [K].sPPAEEAAsPEk.[E] N-Term(TMT6plex); S9(Phospho); K12(TMT6plex) 2 P12036 P12036; P12036-2 0 3 0.4805 0 1 1 584.29444 1750.86878 1750.86647 1.32 0.00077 506032.8125 CID (Collision Induced Dissociation) MS2 48.12482 70 139.7 60.0 51.7393 14633 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 135.9 141.4 109.4 149.2 125.9 128.1 163.5 152.6 154.2 137.2 5.39 1 0.0003003 1.362e-05 False High Sequest HT (A2) Unambiguous GDDDEESDEEAVK [K].gDDDEEsDEEAVk.[K] N-Term(TMT6plex); S7(Phospho); K13(TMT6plex) 1 O43395 O43395 0 2 0.5394 0 1 1 988.42549 1975.84371 1975.84216 0.78 0.00077 250209.53125 CID (Collision Induced Dissociation) MS2 0.0 30 150.1 60.0 52.2197 14810 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 143.1 148.8 121.3 165.9 136.4 126.7 192.7 169.6 148.6 147.8 Excluded by Method 5.84 1 0.0003003 1.045e-07 False High Sequest HT (A2) Unambiguous AEASGEAAEEAEDEE [K].aEASGEAAEEAEDEE.[-] N-Term(TMT6plex) 1 P05755 P05755 0 2 0.8403 0 1 1 883.37583 1765.74438 1765.74493 -0.31 -0.00027 913576.3125 CID (Collision Induced Dissociation) MS2 0.0 100 212.1 60.0 51.255 14453 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 50.6 178.0 355.8 67.1 170.0 386.5 78.3 185.6 427.9 221.5 5.26 1 0.0003003 1.909e-08 False High Sequest HT (A2) Unambiguous AVSPTDSTPPSTHTEDSK [K].aVsPTDSTPPSTHTEDSk.[R] N-Term(TMT6plex); S3(Phospho); K18(TMT6plex) 30 P0C2I9 Q99337; P0C2I9; P47100; Q12141; P47098; P0C2J0; Q03619; P0C2I3; Q12269; Q03855; P0C2I2; Q04214; Q99231; Q12414; Q12088; Q07793; Q04711; Q12316; O13535; Q04670; Q12273; Q03612; Q92393; Q12193; P0C2I5; Q12490; Q03434; O13527; Q12112; P0C2I7 0 3 0.0586 0 1 1 799.0524 2395.14263 2395.14304 -0.17 -0.00013 625044.5 CID (Collision Induced Dissociation) MS2 0.0 40 43.6 60.0 50.89 14317 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 14.9 37.4 64.1 16.4 35.6 82.0 22.6 41.9 73.9 47.3 Excluded by Method 3.24 1 0.0003003 0.0001804 False High Sequest HT (A2) Unambiguous AEASGEAAEEAEDEE [K].aEAsGEAAEEAEDEE.[-] N-Term(TMT6plex); S4(Phospho) 1 P05755 P05755 0 2 0.8287 0 1 1 923.35997 1845.71266 1845.71126 0.76 0.0007 867257.625 CID (Collision Induced Dissociation) MS2 0.0 90 24.0 60.0 54.2026 15545 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 7.0 20.6 33.9 8.1 22.8 46.0 8.0 24.5 43.1 26.4 3.56 1 0.0003003 0.0002661 False High Sequest HT (A2) Unambiguous TDLQGSASPSK [K].tDLQGSAsPSk.[V] N-Term(TMT6plex); S8(Phospho); K11(TMT6plex) 2 Q6VY07 Q6VY07; Q6VY07-2 0 3 0.1511 0 1 1 543.61469 1628.82951 1628.82969 -0.11 -6e-05 2906859.25 CID (Collision Induced Dissociation) MS2 10.63241 60 84.7 60.0 53.4954 15283 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 82.0 90.5 58.3 90.9 84.5 76.7 100.0 89.0 89.3 86.2 Excluded by Method 4.7 1 0.0003003 1.758e-05 False High Sequest HT (A2) Unambiguous RGSIGENQIK [K].rGsIGENQIk.[D] N-Term(TMT6plex); S3(Phospho); K10(TMT6plex) 2 Q05682-4 Q05682-4; Q05682-5 1 3 0.3778 0 1 1 547.30261 1639.89328 1639.89329 -0.01 0.0 31298996.0 CID (Collision Induced Dissociation) MS2 3.804335 60 113.5 6.929 52.2789 14831 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 126.5 114.7 82.1 110.2 95.3 104.2 135.0 127.4 124.9 115.1 Excluded by Method 3.6 1 0.0003003 7.636e-05 False High Sequest HT (A2) Unambiguous EPEGPPPSPGK [K].ePEGPPPsPGk.[G] N-Term(TMT6plex); S8(Phospho); K11(TMT6plex) 1 P58012 P58012 0 3 0.6165 0 1 1 543.94788 1629.82909 1629.82896 0.08 4e-05 982916.625 CID (Collision Induced Dissociation) MS2 2.11311 50 170.7 60.0 53.2113 15180 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 169.8 178.0 125.5 185.6 164.3 154.0 204.0 188.7 170.5 166.1 Excluded by Method 5.71 1 0.0003003 3.086e-05 False High Sequest HT (A2) Unambiguous ETTESPAETTTGTSTEAPSAAN [K].eTtESPAETTTGTSTEAPSAAN.[-] N-Term(TMT6plex); T3(Phospho) 1 P38205 P38205 0 3 0.0297 0 1 1 821.3598 2462.06485 2462.06568 -0.34 -0.00028 520653.25 CID (Collision Induced Dissociation) MS2 4.236364 70 55.6 60.0 52.7923 15023 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 15.6 46.9 85.4 14.8 49.0 100.1 18.9 58.8 108.8 57.5 5.39 1 0.0003003 1.362e-05 False High Sequest HT (A2) Unambiguous AEDGATPSPSNETPK [K].aEDGATPsPSNETPk.[K] N-Term(TMT6plex); S8(Phospho); K15(TMT6plex) 1 P29966 P29966 0 3 0.1181 0 1 1 680.33027 2038.97624 2038.97345 1.37 0.00093 1250516.75 CID (Collision Induced Dissociation) MS2 33.96979 90 452.6 60.0 52.545 14934 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 473.9 452.3 368.1 493.7 429.3 421.1 553.1 487.8 426.5 420.2 3.98 1 0.0003003 1.193e-05 False High Sequest HT (A2) Unambiguous NEATPEAEQVK [K].nEAtPEAEQVk.[K] N-Term(TMT6plex); T4(Phospho); K11(TMT6plex) 1 Q08245 Q08245 0 2 0.3973 0 1 1 877.44224 1753.87721 1753.87737 -0.09 -8e-05 11831590.0 CID (Collision Induced Dissociation) MS2 35.36947 60 274.3 30.532 52.489 14911 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 63.0 217.7 424.6 76.8 218.6 531.4 93.0 250.1 583.3 284.3 Excluded by Method 3.75 1 0.0003003 9.66e-05 False High Sequest HT (A2) Unambiguous ADESDPVESK [K].aDEsDPVESk.[K] N-Term(TMT6plex); S4(Phospho); K10(TMT6plex) 1 P38174 P38174 0 2 0.383 0 1 1 807.8871 1614.76692 1614.76642 0.31 0.00025 2224071.0 CID (Collision Induced Dissociation) MS2 0.0 50 290.4 60.0 54.796 15768 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 68.1 233.0 438.2 84.9 238.4 536.6 93.3 286.3 625.0 300.4 Excluded by Method 4.36 1 0.0003003 0.000103 False High Sequest HT (A2) Unambiguous RAASEESDVEEDEDK [K].rAASEEsDVEEDEDk.[L] N-Term(TMT6plex); S7(Phospho); K15(TMT6plex) 1 Q04660 Q04660 1 3 0.1239 0 1 1 749.67398 2247.0074 2247.0066 0.35 0.00026 656174.3125 CID (Collision Induced Dissociation) MS2 0.0 40 134.8 60.0 47.093 12931 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 46.9 114.2 187.2 56.9 129.7 218.3 70.8 137.9 256.5 129.2 Excluded by Method 5.73 1 0.0003003 4.781e-06 False High Sequest HT (A2) Unambiguous AEDGATPSPSNETPK [K].aEDGATPSPSNEtPk.[K] N-Term(TMT6plex); T13(Phospho); K15(TMT6plex) 1 P29966 P29966 0 3 0.2348 0 1 1 680.32972 2038.97459 2038.97345 0.56 0.00038 17198612.0 CID (Collision Induced Dissociation) MS2 4.261799 60 165.7 15.536 49.2561 13721 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 179.6 160.4 123.0 186.4 145.4 146.2 192.3 184.1 168.3 170.9 Excluded by Method 5.41 1 0.0003003 1.844e-07 False High Sequest HT (A2) Unambiguous GRLVREDENDASDDEDDDEK [K].gRLVREDENDAsDDEDDDEk.[R] N-Term(TMT6plex); S12(Phospho); K20(TMT6plex) 4 Q9Y5B6 Q9Y5B6-4; Q9Y5B6-3; Q9Y5B6; Q9Y5B6-2 2 4 0.5994 0 1 1 715.81807 2860.25046 2860.2522 -0.61 -0.00044 429840.3125 CID (Collision Induced Dissociation) MS2 0.0 40 59.1 60.0 47.4085 13046 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 65.0 61.3 42.6 70.8 50.7 53.8 62.0 62.8 62.9 59.1 Excluded by Method 6.69 1 0.0003003 7.628e-08 False High Sequest HT (A2) Unambiguous AEASGEAADEADEADEE [K].aEASGEAADEADEADEE.[-] N-Term(TMT6plex) 1 O13516 O13516 0 2 0.8122 0 1 1 969.40275 1937.79823 1937.79333 2.53 0.00245 130873.859375 CID (Collision Induced Dissociation) MS2 0.0 100 59.6 60.0 48.3368 13385 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 13.8 45.2 90.4 19.6 49.7 120.3 21.0 55.7 124.1 55.7 5.59 1 0.0003003 5.84e-08 False High Sequest HT (A2) Unambiguous EEPAAAGSGAASPSAAEK [K].eEPAAAGsGAASPSAAEk.[G] N-Term(TMT6plex); S8(Phospho); K18(TMT6plex) 1 P29966 P29966 0 3 0.3363 0 1 1 713.68392 2139.0372 2139.03711 0.04 3e-05 1593090.625 CID (Collision Induced Dissociation) MS2 5.552043 90 24.6 60.0 54.9299 15817 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 25.2 22.9 19.2 27.7 24.9 24.2 25.2 26.4 24.6 26.0 4.49 1 0.0003003 2.728e-06 False High Sequest HT (A2) Unambiguous RIACEEEFSDSEEEGEGGRK [K].rIAcEEEFsDsEEEGEGGRk.[N] N-Term(TMT6plex); C4(Carbamidomethyl); S9(Phospho); S11(Phospho); K20(TMT6plex) 1 Q13547 Q13547 2 4 0.5771 0 1 1 733.81805 2932.25036 2932.24733 1.03 0.00076 700726.75 CID (Collision Induced Dissociation) MS2 2.07288 40 50.1 60.0 61.4196 18225 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 51.9 43.7 42.2 59.1 41.6 50.6 58.3 51.5 55.0 47.3 Excluded by Method 4.28 1 0.0003003 0.0004385 False High Sequest HT (A2) Unambiguous ELDQDMVTEDEDDPGSHK [K].eLDQDmVtEDEDDPGSHk.[R] N-Term(TMT6plex); M6(Oxidation); T8(Phospho); K18(TMT6plex) 2 Q9NRL2 Q9NRL2; Q9NRL2-2 0 3 0.576 0 1 1 872.04766 2614.12843 2614.12679 0.62 0.00054 1596817.625 CID (Collision Induced Dissociation) MS2 0.0 20 77.8 60.0 61.4095 18221 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 90.3 74.8 84.3 50.0 65.7 84.2 89.2 91.1 59.1 89.7 Excluded by Method 4.34 1 0.0003003 3.562e-06 False High Sequest HT (A2) Unambiguous AEGEPQEESPLK [K].aEGEPQEEsPLk.[S] N-Term(TMT6plex); S9(Phospho); K12(TMT6plex) 4 Q9NYF8 Q9NYF8-3; Q9NYF8-4; Q9NYF8; Q9NYF8-2 0 3 0.4828 0 1 1 617.97592 1851.9132 1851.91415 -0.51 -0.00032 11705281.0 CID (Collision Induced Dissociation) MS2 4.277274 70 79.2 9.642 60.8725 18022 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 77.5 84.7 55.9 86.5 72.8 75.2 85.7 86.1 85.2 82.6 5.22 1 0.0003003 0.0004267 False High Sequest HT (A2) Unambiguous VEDNLSEDDSTMK [K].vEDNLsEDDSTmk.[E] N-Term(TMT6plex); S6(Phospho); M12(Oxidation); K13(TMT6plex) 1 P47116 P47116 0 3 0.3207 0 1 1 679.64301 2036.91448 2036.91355 0.45 0.00031 1719283.5 CID (Collision Induced Dissociation) MS2 11.01604 40 132.0 60.0 64.7794 19477 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 111.6 130.9 141.9 111.1 123.0 165.8 131.6 127.2 151.8 125.2 Excluded by Method 2.9 1 0.0003003 0.000322 False High Sequest HT (A2) Unambiguous YRIQEQESSGEEDSDLSPEEREK [K].yRIQEQESsGEEDSDLSPEEREk.[K] N-Term(TMT6plex); S9(Phospho); K23(TMT6plex) 2 Q6NXS1 Q6NXS1; P41236 2 4 0.0304 0 1 1 820.38312 3278.51064 3278.51021 0.13 0.00011 1467405.625 CID (Collision Induced Dissociation) MS2 22.12743 30 28.5 60.0 64.7515 19466 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 21.1 29.1 25.2 28.9 24.2 25.7 29.1 39.3 32.9 29.6 Excluded by Method 4.6 1 0.0003003 6.848e-06 False High Sequest HT (A2) Unambiguous WDGSEEDEDNSK [K].wDGsEEDEDNSk.[K] N-Term(TMT6plex); S4(Phospho); K12(TMT6plex) 1 O60841 O60841 0 3 0.3177 0 1 1 650.27885 1948.82201 1948.82137 0.33 0.00021 783109.125 CID (Collision Induced Dissociation) MS2 41.44407 60 125.9 60.0 64.4357 19350 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 121.2 117.0 96.8 128.0 123.7 129.1 151.4 125.4 129.2 137.1 Excluded by Method 4.91 1 0.0003003 1.381e-05 False High Sequest HT (A2) Unambiguous EYDVDMEDSDDEK [K].eYDVDmEDsDDEk.[D] N-Term(TMT6plex); M6(Oxidation); S9(Phospho); K13(TMT6plex) 1 P53313 P53313 0 3 0.5506 0 1 1 715.29463 2143.86935 2143.86666 1.25 0.0009 754211.4375 CID (Collision Induced Dissociation) MS2 0.0 60 76.5 60.0 62.494 18627 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 21.2 61.1 115.8 30.8 62.4 128.8 33.5 72.9 164.0 74.1 Excluded by Method 5.63 1 0.0003003 8.483e-06 False High Sequest HT (A2) Unambiguous DAPTSPASVASSSSTPSSK [K].dAPTsPASVASSSSTPSSk.[T] N-Term(TMT6plex); S5(Phospho); K19(TMT6plex) 7 Q04726 Q04726-5; Q04726-3; Q04726-2; Q04726-4; Q04726-7; Q04726-6; Q04726 0 3 0.0063 0 1 1 768.0456 2302.12225 2302.12157 0.3 0.00023 2969948.5 CID (Collision Induced Dissociation) MS2 4.134989 40 145.9 60.0 63.0844 18845 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 151.7 144.8 125.5 158.0 144.0 151.6 156.2 143.7 135.5 147.7 Excluded by Method 4.76 1 0.0003003 5.289e-08 False High Sequest HT (A2) Unambiguous EDQEEALSDNEPESSEK [K].eDQEEALsDNEPESSEk.[F] N-Term(TMT6plex); S8(Phospho); K17(TMT6plex) 1 P34250 P34250 0 3 0.4204 0 1 1 825.36736 2474.08754 2474.08597 0.63 0.00052 2052980.0 CID (Collision Induced Dissociation) MS2 0.0 60 75.5 60.0 60.4472 17864 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 16.4 61.5 106.4 21.8 69.3 145.6 29.9 74.8 151.9 77.8 Excluded by Method 5.4 1 0.0003003 5.862e-06 False High Sequest HT (A2) Unambiguous VVRNNSITSATSK [K].vVRNNsITSATSk.[T] N-Term(TMT6plex); S6(Phospho); K13(TMT6plex) 1 Q05050 Q05050 1 3 0.2396 0 1 1 639.01904 1915.04257 1915.04141 0.6 0.00039 3663735.25 CID (Collision Induced Dissociation) MS2 4.827443 50 56.1 54.852 56.6266 16443 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 15.9 44.8 86.9 23.5 49.8 104.9 18.9 57.0 107.0 52.4 Excluded by Method 3.59 1 0.0003003 0.0002828 False High Sequest HT (A2) Unambiguous EVTPESGPPK [K].eVtPESGPPk.[V] N-Term(TMT6plex); T3(Phospho); K10(TMT6plex) 4 P46087 P46087-3; P46087-2; P46087; P46087-4 0 2 0.2199 0 1 1 789.91258 1578.81788 1578.81806 -0.12 -9e-05 1172366.125 CID (Collision Induced Dissociation) MS2 6.018803 50 302.7 60.0 56.2411 16300 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 310.3 302.9 242.8 298.7 297.8 294.4 330.9 324.8 298.3 325.7 Excluded by Method 3.32 1 0.0003003 0.0003681 False High Sequest HT (A2) Unambiguous STSAPQMSPGSSDNQSSSPQPAQQK [K].sTSAPQMsPGSSDNQSSSPQPAQQk.[L] N-Term(TMT6plex); S8(Phospho); K25(TMT6plex) 5 Q14157-1 Q14157-1; Q14157-5; Q14157-4; Q14157; Q14157-3 0 3 0.0565 0 1 1 1024.14609 3070.42371 3070.41889 1.57 0.00161 173841.578125 CID (Collision Induced Dissociation) MS2 0.0 60 15.6 60.0 55.6965 16100 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 14.0 16.3 13.7 13.2 16.3 16.9 15.6 15.8 17.8 16.5 Excluded by Method 4.25 1 0.0003003 0.0003196 False High Sequest HT (A2) Unambiguous RESVASGDDRAEEDMDEAIEK [K].rEsVASGDDRAEEDmDEAIEk.[G] N-Term(TMT6plex); S3(Phospho); M15(Oxidation); K21(TMT6plex) 1 P46821 P46821 2 4 0.0668 0 1 1 727.33325 2906.31119 2906.3127 -0.52 -0.00038 323386.0 CID (Collision Induced Dissociation) MS2 8.441985 60 35.6 60.0 55.4726 16016 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 33.7 40.8 31.1 35.7 31.6 41.9 31.5 37.8 34.2 38.1 Excluded by Method 3.89 1 0.0003003 5.521e-05 False High Sequest HT (A2) Unambiguous VNGDASPAAAESGAK [K].vNGDAsPAAAESGAk.[E] N-Term(TMT6plex); S6(Phospho); K15(TMT6plex) 1 P29966 P29966 0 2 0.3233 0 1 1 941.96816 1882.92904 1882.93119 -1.14 -0.00108 1244158.375 CID (Collision Induced Dissociation) MS2 22.12875 20 134.1 60.0 55.7825 16133 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 138.5 134.9 102.1 135.2 125.4 131.9 156.8 135.4 136.0 144.5 Excluded by Method 4.3 1 0.0003003 4.079e-05 False High Sequest HT (A2) Unambiguous RKPSPEPEGEVGPPK [K].rkPsPEPEGEVGPPk.[I] N-Term(TMT6plex); K2(TMT6plex); S4(Phospho); K15(TMT6plex) 2 Q7Z5L9 Q7Z5L9-2; Q7Z5L9 1 3 0.5242 0 1 1 791.1041 2371.29776 2371.29897 -0.51 -0.00041 200956.9375 CID (Collision Induced Dissociation) MS2 51.82631 40 54.1 60.0 56.8416 16523 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 52.3 52.4 46.8 54.9 60.3 50.8 65.2 52.6 50.3 55.0 Excluded by Method 5.38 1 0.0003003 0.0001717 False High Sequest HT (A2) Unambiguous NEPEDEEEEEEEEDEDEEEEDEDEE [K].nEPEDEEEEEEEEDEDEEEEDEDEE.[-] N-Term(TMT6plex) 1 P26583 P26583 0 3 0.9826 0 1 1 1119.73007 3357.17565 3357.17785 -0.66 -0.00074 785672.4375 CID (Collision Induced Dissociation) MS2 0.0 90 8.2 60.0 60.0615 17712 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 8.0 3.3 7.5 6.0 9.2 8.8 8.8 9.9 11.5 9.4 5.16 1 0.0003003 3.092e-06 False High Sequest HT (A2) Unambiguous LYERRGGGSGGGEESEGEEVDED [K].lYERRGGGsGGGEEsEGEEVDED.[-] N-Term(TMT6plex); S9(Phospho); S15(Phospho) 1 Q96QR8 Q96QR8 2 3 0.1799 0 1 1 934.70389 2802.09711 2802.0978 -0.25 -0.00023 436486.90625 CID (Collision Induced Dissociation) MS2 0.0 50 59.2 60.0 59.4308 17478 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 54.8 59.0 56.8 59.5 54.6 53.8 70.6 66.0 61.7 55.0 Excluded by Method 4.28 1 0.0003003 0.0003177 False High Sequest HT (A2) Unambiguous KNEPEDEEEEEEEEDEDEEEEDEDEE [K].kNEPEDEEEEEEEEDEDEEEEDEDEE.[-] N-Term(TMT6plex); K1(TMT6plex) 1 P26583 P26583 1 3 0.8179 0 1 1 1238.81841 3714.44067 3714.43575 1.32 0.00164 796677.75 CID (Collision Induced Dissociation) MS2 2.751836 70 69.1 60.0 58.0374 16964 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 71.3 68.4 46.0 73.9 64.6 70.6 87.7 70.1 62.2 76.0 6.26 1 0.0003003 6.488e-09 False High Sequest HT (A2) Unambiguous RAAVGQESPGGLEAGNAK [K].rAAVGQEsPGGLEAGNAk.[A] N-Term(TMT6plex); S8(Phospho); K18(TMT6plex) 1 Q29RF7 Q29RF7 1 3 0.4962 0 1 1 750.72647 2250.16486 2250.16438 0.21 0.00016 10414056.0 CID (Collision Induced Dissociation) MS2 0.7785502 30 121.0 16.862 57.8171 16883 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 113.3 126.3 90.4 120.9 114.1 125.9 140.0 127.9 113.9 137.3 Excluded by Method 5.3 1 0.0003003 5.639e-08 False High Sequest HT (A2) Unambiguous GGKDDEIDEEDDSEEAK [K].gGkDDEIDEEDDsEEAk.[A] N-Term(TMT6plex); K3(TMT6plex); S13(Phospho); K17(TMT6plex) 1 P32892 P32892 1 3 0.5052 0 1 1 883.40671 2648.20559 2648.20671 -0.42 -0.00037 383274.5 CID (Collision Induced Dissociation) MS2 26.84257 60 29.9 60.0 57.7891 16873 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 9.2 27.4 37.9 10.9 24.2 48.8 15.9 30.1 61.1 33.3 Excluded by Method 3.88 1 0.0003003 9.428e-05 False High Sequest HT (A2) Unambiguous EESTTEATGNESQDATK [K].eESTTEATGNEsQDATk.[V] N-Term(TMT6plex); S12(Phospho); K17(TMT6plex) 1 P32499 P32499 0 3 0.2435 0 1 1 779.35809 2336.05971 2336.05428 2.32 0.00181 879545.3125 CID (Collision Induced Dissociation) MS2 19.37536 100 40.8 60.0 45.2831 12275 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 13.7 33.1 61.3 15.4 35.1 79.2 15.9 35.7 75.8 43.1 4.97 1 0.0003003 5.487e-05 False High Sequest HT (A2) Unambiguous STSAPQMSPGSSDNQSSSPQPAQQK [K].sTSAPQmsPGSSDNQSSSPQPAQQk.[L] N-Term(TMT6plex); M7(Oxidation); S8(Phospho); K25(TMT6plex) 5 Q14157-1 Q14157-1; Q14157-5; Q14157-4; Q14157; Q14157-3 0 4 0.065 0 1 1 772.36225 3086.42716 3086.41381 4.32 0.00334 234504.234375 CID (Collision Induced Dissociation) MS2 0.0 80 109.3 60.0 45.1298 12220 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 111.9 100.4 88.6 105.8 107.4 105.2 134.6 124.7 107.5 106.9 5.54 1 0.0003003 0.0001457 False High Sequest HT (A2) Unambiguous YAAAAPAATSAASGDAAPAEEAAAEEEEESDDDMGFGLFD [K].yAAAAPAATSAASGDAAPAEEAAAEEEEEsDDDmGFGLFD.[-] N-Term(TMT6plex); S30(Phospho); M34(Oxidation) 1 P05317 P05317 0 4 0.4004 0 1 1 1062.19334 4245.75152 4245.73286 4.39 0.00466 3435219.5 CID (Collision Induced Dissociation) MS2 0.0 60 30.0 21.008 129.7926 44026 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 8.8 20.3 45.3 9.0 26.4 54.3 11.1 32.9 59.6 32.6 Excluded by Method 4.77 1 0.0003003 0.0002028 False High Sequest HT (A2) Unambiguous VRTDTTYPAGFMDVITLDATNENFRLVYDVK [K].vRTDTTYPAGFmDVITLDATNENFRLVYDVk.[G] N-Term(TMT6plex); M12(Oxidation); K31(TMT6plex) 1 P0CX35 P0CX35 2 4 0.5573 0 1 1 1010.52774 4039.08914 4039.08869 0.11 0.00011 4401977.0 CID (Collision Induced Dissociation) MS2 45.98464 30 76.5 11.944 127.6512 43053 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 16.5 63.0 106.8 25.5 51.8 158.7 28.4 82.0 157.8 74.0 Excluded by Method 8.2 1 0.0003003 2.38e-09 False High Sequest HT (A2) Unambiguous ELVGPPLAETVFTPK [K].eLVGPPLAETVFtPk.[T] N-Term(TMT6plex); T13(Phospho); K15(TMT6plex) 1 P49792 P49792 0 3 0.2781 0 1 1 712.73021 2136.17606 2136.17578 0.13 9e-05 9199922.0 CID (Collision Induced Dissociation) MS2 3.799387 20 92.6 13.636 127.492 42981 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 93.6 86.8 73.1 98.0 84.5 90.3 111.2 93.9 96.4 98.1 Excluded by Method 3.38 1 0.0003003 0.000158 False High Sequest HT (A2) Unambiguous EEVLSESEAENQQAGAAALAPEIVIK [K].eEVLsESEAENQQAGAAALAPEIVIk.[V] N-Term(TMT6plex); S5(Phospho); K26(TMT6plex) 2 Q9UJX5 Q9UJX5-3; Q9UJX5 0 4 0.1339 0 1 1 809.42011 3234.65859 3234.65469 1.21 0.00098 1071174.25 CID (Collision Induced Dissociation) MS2 26.37404 50 16.5 30.84 127.2496 42874 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 14.4 14.4 14.7 14.1 13.3 25.9 13.0 16.1 24.4 14.3 Excluded by Method 3.81 1 0.0003003 0.0003255 False High Sequest HT (A2) Unambiguous LSNSSLSNLFK [K].lsNSSLSNLFk.[K] N-Term(TMT6plex); S2(Phospho); K11(TMT6plex) 1 P13259 P13259 0 3 0.0079 0 1 1 583.31817 1747.93996 1747.93957 0.22 0.00013 4593342.5 CID (Collision Induced Dissociation) MS2 0.0 50 210.2 58.367 127.79 43115 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 51.9 165.9 331.1 68.5 172.2 400.9 78.8 188.7 426.1 217.5 Excluded by Method 3.78 1 0.0003003 0.0002563 False High Sequest HT (A2) Unambiguous ALVLELDEMSNGSEIQDALEEISGQK [K].aLVLELDEmsNGSEIQDALEEISGQk.[T] N-Term(TMT6plex); M9(Oxidation); S10(Phospho); K26(TMT6plex) 2 P17695 P17695; P17695-2 0 3 0.0751 0 1 1 1124.88871 3372.65157 3372.65337 -0.53 -0.0006 2258619.75 CID (Collision Induced Dissociation) MS2 0.0 70 36.9 22.996 130.0174 44124 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 13.5 33.5 51.9 9.0 28.6 70.6 14.7 33.7 76.8 36.5 3.73 1 0.0003003 1.091e-05 False High Sequest HT (A2) Unambiguous EEEAAEESDDDMGFGLFD [K].eEEAAEEsDDDMGFGLFD.[-] N-Term(TMT6plex); S8(Phospho) 2 P05319 P10622; P05319 0 3 0.7756 0 1 1 772.29807 2314.87967 2314.8784 0.55 0.00042 1549760.875 CID (Collision Induced Dissociation) MS2 0.0 60 54.7 60.0 131.7206 44835 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 18.0 47.7 83.5 18.2 40.6 104.0 21.3 52.3 97.4 64.4 Excluded by Method 4.5 1 0.0003003 0.0001971 False High Sequest HT (A2) Unambiguous YAAAAPAATSAASGDAAPAEEAAAEEEEESDDDMGFGLFD [K].yAAAAPAATSAASGDAAPAEEAAAEEEEEsDDDmGFGLFD.[-] N-Term(TMT6plex); S30(Phospho); M34(Oxidation) 1 P05317 P05317 0 4 0.3228 0 1 1 1062.18858 4245.73248 4245.73286 -0.09 -0.0001 1406208.75 CID (Collision Induced Dissociation) MS2 10.35455 70 18.3 25.46 131.1532 44608 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 3.3 15.9 23.3 6.0 14.4 33.3 7.7 18.0 41.0 19.9 3.81 1 0.0003003 0.0002582 False High Sequest HT (A2) Unambiguous EEEAAEESDDDMGFGLFD [K].eEEAAEEsDDDmGFGLFD.[-] N-Term(TMT6plex); S8(Phospho); M12(Oxidation) 2 P05319 P10622; P05319 0 2 0.7975 0 1 1 1165.94128 2330.87527 2330.87332 0.84 0.00098 6713477.0 CID (Collision Induced Dissociation) MS2 43.54589 70 39.9 20.847 130.2839 44239 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 8.1 29.3 52.4 12.4 33.0 76.4 12.3 44.4 86.5 44.2 4.84 1 0.0003003 3.748e-05 False High Sequest HT (A2) Unambiguous NMITGTSQADCAILIIAGGVGEFEAGISK [K].nmITGTSQADcAILIIAGGVGEFEAGISk.[D] N-Term(TMT6plex); M2(Oxidation); C11(Carbamidomethyl); K29(TMT6plex) 1 P02994 P02994 0 4 0.2944 0 1 1 850.19912 3397.77463 3397.77475 -0.03 -3e-05 4221678.0 CID (Collision Induced Dissociation) MS2 0.0 60 17.2 14.891 130.7722 44448 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 7.2 14.8 23.3 9.2 17.1 28.6 8.6 17.0 27.9 18.6 Excluded by Method 2.31 1 0.0003003 0.0004607 False High Sequest HT (A2) Unambiguous DSTLIMQLLRDNLTLWTSENQGDEGDAGEGEN [K].dSTLImQLLRDNLTLWTSENQGDEGDAGEGEN.[-] N-Term(TMT6plex); M6(Oxidation) 2 P31946 P31946-2; P31946 1 4 0.4039 0 1 1 942.44456 3766.75642 3766.75512 0.35 0.00033 2994276.0 CID (Collision Induced Dissociation) MS2 5.094781 40 29.0 8.579 130.706 44422 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 26.5 25.7 29.2 18.3 31.7 35.2 29.0 33.2 32.5 29.1 Excluded by Method 4.06 1 0.0003003 0.0002376 False High Sequest HT (A2) Unambiguous EEEAAEESDDDMGFGLFD [K].eEEAAEEsDDDMGFGLFD.[-] N-Term(TMT6plex); S8(Phospho) 2 P05319 P10622; P05319 0 2 0.8274 0 1 1 1157.94311 2314.87895 2314.8784 0.24 0.00028 1308289.375 CID (Collision Induced Dissociation) MS2 50.21339 60 50.4 25.56 130.6016 44376 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 14.2 38.8 73.0 15.4 38.3 99.7 15.9 48.8 100.4 59.1 Excluded by Method 4.81 1 0.0003003 0.0001007 False High Sequest HT (A2) Unambiguous SLQPFDDLLGDFNK [K].sLQPFDDLLGDFNk.[V] N-Term(TMT6plex); S1(Phospho); K14(TMT6plex) 1 Q06336 Q06336 0 3 0.3406 0 1 1 716.36613 2147.08383 2147.08261 0.57 0.00041 1054829.125 CID (Collision Induced Dissociation) MS2 68.33602 30 73.7 60.0 130.5691 44363 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 22.2 59.8 94.7 29.9 63.3 136.3 33.3 77.1 140.1 80.3 Excluded by Method 3.2 1 0.0003003 0.0001812 False High Sequest HT (A2) Unambiguous APLATGEDDDDEVPDLVENFDEASK [K].aPLATGEDDDDEVPDLVENFDEASk.[N] N-Term(TMT6plex); K25(TMT6plex) 2 P20290 P20290-2; P20290 0 3 0.5342 0 1 1 1050.50634 3149.50446 3149.50503 -0.18 -0.00019 3084127.25 CID (Collision Induced Dissociation) MS2 10.45553 50 47.5 20.293 127.1724 42840 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 51.0 45.5 38.5 48.7 35.6 49.8 53.7 51.6 45.7 54.5 Excluded by Method 6.44 1 0.0003003 7.741e-08 False High Sequest HT (A2) Unambiguous ITEIEDGQDGGVTPYSNESGLVNADLDVK [K].iTEIEDGQDGGVtPYSNESGLVNADLDVk.[D] N-Term(TMT6plex); T13(Phospho); K29(TMT6plex) 1 P04050 P04050 0 3 0.009 0 1 1 1191.90792 3573.70921 3573.72495 -4.4 -0.00525 454650.0625 CID (Collision Induced Dissociation) MS2 15.96014 60 47.6 60.0 125.2019 42104 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 9.1 36.6 71.5 14.5 38.2 89.1 20.0 43.9 105.1 47.7 Excluded by Method 7.75 1 0.0003003 9.617e-08 False High Sequest HT (A2) Unambiguous YAAAAPAATSAASGDAAPAEEAAAEEEEESDDDMGFGLFD [K].yAAAAPAATSAASGDAAPAEEAAAEEEEEsDDDmGFGLFD.[-] N-Term(TMT6plex); S30(Phospho); M34(Oxidation) 1 P05317 P05317 0 4 0.2908 0 1 1 1062.19383 4245.75348 4245.73286 4.85 0.00515 488030.625 CID (Collision Induced Dissociation) MS2 38.56771 30 3.0 60.0 124.3457 41787 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 2.8 2.6 6.7 2.2 8.4 1.8 2.2 3.4 Excluded by Method 3.37 1 0.0003003 0.0002124 False High Sequest HT (A2) Unambiguous EEVTEGQAEATEWAEENADNVEW [K].eEVTEGQAEATEWAEENADNVEW.[-] N-Term(TMT6plex) 1 P46654 P46654 0 3 0.6365 0 1 1 955.75583 2865.25293 2865.25362 -0.24 -0.00023 405839.28125 CID (Collision Induced Dissociation) MS2 8.598962 70 54.4 60.0 125.5758 42239 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 10.9 49.8 90.7 17.9 38.9 112.9 17.8 46.6 102.7 56.0 5.2 1 0.0003003 1.097e-05 False High Sequest HT (A2) Unambiguous FLYGSDSELALPSSK [K].fLYGsDSELALPSSk.[R] N-Term(TMT6plex); S5(Phospho); K15(TMT6plex) 1 P45976 P45976 0 3 0.0111 0 1 1 718.03776 2152.09872 2152.09792 0.37 0.00026 4229480.5 CID (Collision Induced Dissociation) MS2 4.089017 20 184.0 27.805 125.6758 42277 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 39.2 151.4 308.0 56.5 153.6 346.7 67.2 181.5 361.0 175.1 Excluded by Method 4.52 1 0.0003003 4.485e-05 False High Sequest HT (A2) Unambiguous LAAVPAAGPASAGGAAAASGDAAAEEEKEEEAAEESDDDMGFGLFD [K].lAAVPAAGPASAGGAAAASGDAAAEEEkEEEAAEEsDDDmGFGLFD.[-] N-Term(TMT6plex); K28(TMT6plex); S36(Phospho); M40(Oxidation) 1 P05319 P05319 1 4 0.3758 0 1 1 1231.3073 4922.20735 4922.17633 6.3 0.00776 1177382.625 CID (Collision Induced Dissociation) MS2 12.79044 30 21.6 60.0 126.8438 42711 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 3.4 16.4 37.6 3.9 22.4 47.6 7.2 16.9 41.4 18.9 Excluded by Method 5.96 1 0.0003003 3.436e-05 False High Sequest HT (A2) Unambiguous DLVLPTQALPASPALK [K].dLVLPTQALPAsPALk.[N] N-Term(TMT6plex); S12(Phospho); K16(TMT6plex) 1 Q15554 Q15554 0 3 0.5768 0 1 1 724.75351 2172.24597 2172.24453 0.66 0.00048 2187808.0 CID (Collision Induced Dissociation) MS2 2.056868 50 152.9 59.297 126.8289 42704 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 159.5 144.8 123.7 171.8 138.1 151.6 190.8 158.4 136.1 154.6 Excluded by Method 5.34 1 0.0003003 4.345e-06 False High Sequest HT (A2) Unambiguous LSLGQYDNDAGGQLPFSK [K].lsLGQYDNDAGGQLPFSk.[C] N-Term(TMT6plex); S2(Phospho); K18(TMT6plex) 2 Q68CZ2 Q68CZ2; Q68CZ2-2 0 3 0.0366 0 1 1 816.74682 2448.22591 2448.22123 1.91 0.00156 1782631.0 CID (Collision Induced Dissociation) MS2 10.32567 50 101.6 60.0 126.4855 42575 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 97.1 105.6 85.3 114.5 93.4 107.9 109.5 103.8 103.7 95.5 Excluded by Method 4.37 1 0.0003003 1.107e-08 False High Sequest HT (A2) Unambiguous SLGVSPDSLEHTFSGFSQGSSIEDDQDAISNQEK [K].sLGVSPDSLEHTFSGFSQGSSIEDDQDAIsNQEk.[K] N-Term(TMT6plex); S30(Phospho); K34(TMT6plex) 1 Q05050 Q05050 0 4 0.4601 0 1 1 1038.23645 4149.92399 4149.91779 1.49 0.00155 266378.1875 CID (Collision Induced Dissociation) MS2 0.0 50 13.9 60.0 126.3653 42531 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 2.5 13.5 24.0 4.1 12.4 25.1 3.4 14.2 27.8 12.0 Excluded by Method 5.76 1 0.0003003 6.406e-07 False High Sequest HT (A2) Unambiguous SFPTFQQLYEPGTK [K].sFPTFQQLYEPGTk.[I] N-Term(TMT6plex); S1(Phospho); K14(TMT6plex) 1 P47025 P47025 0 3 0.0232 0 1 1 727.70535 2181.10149 2181.10334 -0.85 -0.00062 581044.1875 CID (Collision Induced Dissociation) MS2 4.226764 40 106.6 60.0 126.1124 42437 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 27.6 83.7 168.8 30.7 98.0 209.4 34.6 93.0 203.8 116.7 Excluded by Method 4.75 1 0.0003003 1.417e-05 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVssDEEF.[-] N-Term(TMT6plex); S8(Phospho); S9(Phospho) 1 P16521 P16521 0 2 0.1319 0 1 1 925.3551 1849.70293 1849.70195 0.53 0.00049 3410403.5 CID (Collision Induced Dissociation) MS2 5.457572 40 106.4 33.426 125.831 42334 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 24.5 86.6 164.7 27.2 89.8 221.3 32.1 86.4 225.0 106.5 Excluded by Method 4.55 1 0.0003003 2.415e-05 False High Sequest HT (A2) Unambiguous YAAAAPAATSAASGDAAPAEEAAAEEEEESDDDMGFGLFD [K].yAAAAPAATSAASGDAAPAEEAAAEEEEEsDDDmGFGLFD.[-] N-Term(TMT6plex); S30(Phospho); M34(Oxidation) 1 P05317 P05317 0 4 0.3285 0 1 1 1062.19554 4245.76031 4245.73286 6.46 0.00686 722344.6875 CID (Collision Induced Dissociation) MS2 10.51202 50 16.5 43.149 132.5482 45164 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 4.2 17.0 25.8 1.5 11.5 31.6 7.9 15.3 35.8 14.8 Excluded by Method 5.48 1 0.0003003 0.0004636 False High Sequest HT (A2) Unambiguous VQGEAVSNIQENTQTPTVQEESEEEEVDETGVEVK [K].vQGEAVSNIQENTQTPTVQEEsEEEEVDETGVEVk.[D] N-Term(TMT6plex); S22(Phospho); K35(TMT6plex) 3 Q13765 Q13765; E9PAV3; E9PAV3-2 0 4 0.0629 0 1 1 1100.51837 4399.05165 4399.06016 -1.93 -0.00213 404957.75 CID (Collision Induced Dissociation) MS2 13.70876 50 27.6 60.0 132.819 45263 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 27.9 24.9 19.7 30.1 26.5 27.2 27.8 33.3 30.9 27.5 Excluded by Method 5.25 1 0.0003003 3.392e-05 False High Sequest HT (A2) Unambiguous HSPTEDEESAK [K].hsPTEDEESAk.[A] N-Term(TMT6plex); S2(Phospho); K11(TMT6plex) 1 P46821 P46821 0 3 0.0055 0 1 1 589.94525 1767.82121 1767.82025 0.55 0.00032 226749.4375 CID (Collision Induced Dissociation) MS2 0.0 60 70.7 60.0 34.3531 8345 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 69.5 69.9 54.4 72.8 71.3 64.0 81.9 78.6 75.6 69.3 Excluded by Method 3.63 1 0.0003003 0.0002511 False High Sequest HT (A2) Unambiguous NAEEESESEAEEGD [K].nAEEEsESEAEEGD.[-] N-Term(TMT6plex); S6(Phospho) 4 Q7L1Q6 Q7L1Q6-2; Q7L1Q6-4; Q7L1Q6-3; Q7L1Q6 0 2 0.1821 0 1 1 917.34069 1833.6741 1833.67487 -0.42 -0.00039 149289.5625 CID (Collision Induced Dissociation) MS2 16.342 70 91.4 60.0 37.1665 9352 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 95.2 94.5 75.0 93.5 84.8 86.6 100.8 93.2 101.4 89.1 3.9 1 0.0003003 0.000418 False High Sequest HT (A2) Unambiguous DDSHSAEDSEDEK [K].dDSHSAEDsEDEk.[E] N-Term(TMT6plex); S9(Phospho); K13(TMT6plex) 2 Q9H1E3 Q9H1E3; Q9H1E3-2 0 3 0.4205 0 1 1 667.94838 2001.8306 2001.83266 -1.03 -0.00069 76009.3203125 CID (Collision Induced Dissociation) MS2 41.62357 30 153.0 60.0 31.0536 7181 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 164.1 160.5 119.5 170.0 131.2 138.9 178.5 169.9 155.4 142.1 Excluded by Method 6.16 1 0.0003003 2.29e-06 False High Sequest HT (A2) Unambiguous DHNSEDEDEDK [K].dHNsEDEDEDk.[Y] N-Term(TMT6plex); S4(Phospho); K11(TMT6plex) 1 O95391 O95391 0 3 0.4816 0 1 1 624.26259 1870.77322 1870.77442 -0.64 -0.0004 65919.6875 CID (Collision Induced Dissociation) MS2 0.0 70 18.0 60.0 28.9403 6442 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 23.8 20.6 14.0 21.9 11.8 13.1 18.9 16.6 18.1 21.2 3.8 1 0.0003003 0.0004402 False High Sequest HT (A2) Unambiguous YAAAAPAATSAASGDAAPAEEAAAEEEEESDDDMGFGLFD [K].yAAAAPAATSAASGDAAPAEEAAAEEEEEsDDDmGFGLFD.[-] N-Term(TMT6plex); S30(Phospho); M34(Oxidation) 1 P05317 P05317 0 4 0.3647 0 1 1 1062.19261 4245.74859 4245.73286 3.7 0.00393 353915.40625 CID (Collision Induced Dissociation) MS2 8.008565 60 12.1 60.0 147.3438 50286 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 9.9 23.2 1.9 8.9 21.7 4.1 13.1 24.5 13.2 Excluded by Method 4.36 1 0.0003003 0.0003931 False High Sequest HT (A2) Unambiguous YAAAAPAATSAASGDAAPAEEAAAEEEEESDDDMGFGLFD [K].yAAAAPAATSAASGDAAPAEEAAAEEEEEsDDDmGFGLFD.[-] N-Term(TMT6plex); S30(Phospho); M34(Oxidation) 1 P05317 P05317 0 4 0.2733 0 1 1 1062.19261 4245.74859 4245.73286 3.7 0.00393 482497.5 CID (Collision Induced Dissociation) MS2 9.007803 40 8.9 60.0 146.273 49917 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 1.4 3.2 15.1 7.5 17.1 1.3 12.6 20.9 10.1 Excluded by Method 5.16 1 0.0003003 0.0002657 False High Sequest HT (A2) Unambiguous NAEEESESEAEEGD [K].nAEEEsEsEAEEGD.[-] N-Term(TMT6plex); S6(Phospho); S8(Phospho) 4 Q7L1Q6 Q7L1Q6-2; Q7L1Q6-4; Q7L1Q6-3; Q7L1Q6 0 2 0.9707 0 1 1 957.3244 1913.64152 1913.6412 0.17 0.00016 135753.71875 CID (Collision Induced Dissociation) MS2 0.0 60 8.0 60.0 37.7208 9549 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 8.8 9.8 7.2 7.8 7.9 4.0 8.5 8.6 9.4 7.6 Excluded by Method 3.41 1 0.0003003 0.0002389 False High Sequest HT (A2) Unambiguous DGQTDSAAEESSSQETSK [K].dGQtDSAAEESSSQETSk.[GD] N-Term(TMT6plex); T4(Phospho); K18(TMT6plex) 4 P55201 P55201-3; P55201-4; P55201-2; P55201 0 3 0.011 0 1 1 799.02316 2395.05493 2395.05501 -0.03 -3e-05 454523.03125 CID (Collision Induced Dissociation) MS2 0.0 70 9.7 60.0 43.5284 11641 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 9.6 11.3 7.8 12.3 10.2 8.7 10.1 9.1 9.5 8.5 3.65 1 0.0003003 3.463e-05 False High Sequest HT (A2) Unambiguous HIVSNDSSDSDDESHEPK [K].hIVSNDSSDsDDESHEPk.[G] N-Term(TMT6plex); S10(Phospho); K18(TMT6plex) 1 P47712 P47712 0 3 0.1624 0 1 1 846.04635 2536.1245 2536.12409 0.16 0.00014 342130.375 CID (Collision Induced Dissociation) MS2 0.0 40 69.5 60.0 42.3269 11205 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 40.1 67.6 68.3 60.2 62.3 87.0 73.2 72.1 118.1 46.3 Excluded by Method 6.96 1 0.0003003 2.5e-06 False High Sequest HT (A2) Unambiguous ANADANEDNNVDEK [K].aNADANEDNNVDEk.[Q] N-Term(TMT6plex); K14(TMT6plex) 1 P49090 P49090 0 3 0.5168 0 1 1 659.65726 1976.95722 1976.95615 0.54 0.00036 574425.625 CID (Collision Induced Dissociation) MS2 14.25532 60 156.7 60.0 42.3195 11202 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 45.6 111.2 245.0 52.6 136.9 290.6 57.4 149.4 310.3 168.3 Excluded by Method 4.16 1 0.0003003 1.267e-05 False High Sequest HT (A2) Unambiguous RGSIGENQGEEK [K].rGsIGENQGEEk.[G] N-Term(TMT6plex); S3(Phospho); K12(TMT6plex) 3 Q05682-2 Q05682-3; Q05682-6; Q05682-2 1 3 0.5068 0 1 1 614.64401 1841.91749 1841.91588 0.87 0.00054 5952071.0 CID (Collision Induced Dissociation) MS2 4.135568 60 117.8 60.0 39.3529 10139 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 110.6 115.1 83.9 130.8 118.1 121.6 125.2 133.6 122.8 116.6 Excluded by Method 5.15 1 0.0003003 3.61e-05 False High Sequest HT (A2) Unambiguous EEEAAEESDDDMGFGLFD [K].eEEAAEEsDDDMGFGLFD.[-] N-Term(TMT6plex); S8(Phospho) 2 P05319 P10622; P05319 0 2 0.6766 0 1 1 1157.94153 2314.87578 2314.8784 -1.13 -0.00131 489816.9375 CID (Collision Induced Dissociation) MS2 46.48319 60 28.9 60.0 137.8 47006 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 10.0 25.0 40.8 6.4 22.3 58.5 10.5 26.7 58.7 29.7 Excluded by Method 3.03 1 0.0003003 0.0003731 False High Sequest HT (A2) Unambiguous ALVLELDEMSNGSEIQDALEEISGQK [K].aLVLELDEmSNGsEIQDALEEISGQk.[T] N-Term(TMT6plex); M9(Oxidation); S13(Phospho); K26(TMT6plex) 2 P17695 P17695; P17695-2 0 3 0.0145 0 1 1 1124.88956 3372.65413 3372.65337 0.23 0.00025 308741.03125 CID (Collision Induced Dissociation) MS2 58.15659 70 19.8 60.0 136.1727 46446 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 7.4 15.5 29.7 9.2 13.3 38.8 10.4 17.4 36.5 19.7 2.75 1 0.0003003 0.0004755 False High Sequest HT (A2) Unambiguous YAAAAPAATSAASGDAAPAEEAAAEEEEESDDDMGFGLFD [K].yAAAAPAATSAASGDAAPAEEAAAEEEEEsDDDmGFGLFD.[-] N-Term(TMT6plex); S30(Phospho); M34(Oxidation) 1 P05317 P05317 0 4 0.2819 0 1 1 1062.19322 4245.75103 4245.73286 4.28 0.00454 790942.5625 CID (Collision Induced Dissociation) MS2 50.65611 60 22.8 60.0 135.3136 46154 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 7.2 18.9 35.1 6.3 20.4 44.3 6.1 21.1 46.5 22.2 Excluded by Method 5.57 1 0.0003003 1.594e-05 False High Sequest HT (A2) Unambiguous VQGEAVSNIQENTQTPTVQEESEEEEVDETGVEVK [K].vQGEAVSNIQENTQTPTVQEEsEEEEVDETGVEVk.[D] N-Term(TMT6plex); S22(Phospho); K35(TMT6plex) 3 Q13765 Q13765; E9PAV3; E9PAV3-2 0 4 0.0186 0 1 1 1100.52252 4399.06825 4399.06016 1.84 0.00202 808671.8125 CID (Collision Induced Dissociation) MS2 0.0 50 19.7 60.0 135.0708 46068 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 26.1 18.8 14.1 23.5 18.0 18.4 21.7 18.9 18.5 19.2 Excluded by Method 4.84 1 0.0003003 1.285e-06 False High Sequest HT (A2) Unambiguous ALVLELDEMSNGSEIQDALEEISGQK [K].aLVLELDEmSNGsEIQDALEEISGQk.[T] N-Term(TMT6plex); M9(Oxidation); S13(Phospho); K26(TMT6plex) 2 P17695 P17695; P17695-2 0 3 0.0084 0 1 1 1124.88724 3372.64717 3372.65337 -1.84 -0.00207 393362.59375 CID (Collision Induced Dissociation) MS2 10.19868 90 19.6 60.0 134.5897 45900 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 2.9 20.7 24.2 10.2 16.2 28.4 8.5 19.6 39.7 25.5 3.56 1 0.0003003 5.921e-05 False High Sequest HT (A2) Unambiguous EEEAAEESDDDMGFGLFD [K].eEEAAEEsDDDMGFGLFD.[-] N-Term(TMT6plex); S8(Phospho) 2 P05319 P10622; P05319 0 2 0.7912 0 1 1 1157.94495 2314.88261 2314.8784 1.82 0.00211 776254.4375 CID (Collision Induced Dissociation) MS2 0.0 70 37.3 60.0 132.8981 45294 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 10.8 35.6 53.6 11.8 31.2 74.3 12.0 35.6 70.4 37.8 3.88 1 0.0003003 0.0001188 False High Sequest HT (A2) Unambiguous EEEAAEESDDDMGFGLFD [K].eEEAAEEsDDDMGFGLFD.[-] N-Term(TMT6plex); S8(Phospho) 2 P05319 P10622; P05319 0 3 0.8558 0 1 1 772.29838 2314.88059 2314.8784 0.94 0.00073 395289.46875 CID (Collision Induced Dissociation) MS2 6.781814 80 27.1 60.0 139.3583 47541 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 7.0 28.7 42.3 11.4 23.7 46.4 11.2 20.8 52.4 26.7 4.37 1 0.0003003 7.008e-06 False High Sequest HT (A2) Unambiguous ALVLELDEMSNGSEIQDALEEISGQK [K].aLVLELDEmsNGSEIQDALEEISGQk.[T] N-Term(TMT6plex); M9(Oxidation); S10(Phospho); K26(TMT6plex) 2 P17695 P17695; P17695-2 0 4 0.084 0 1 1 843.91799 3372.65015 3372.65337 -0.96 -0.00081 732333.25 CID (Collision Induced Dissociation) MS2 26.04527 60 39.0 60.0 139.4661 47578 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 20.1 32.2 49.8 16.6 32.4 60.5 21.8 45.0 68.5 43.2 Excluded by Method 3.69 1 0.0003003 0.0005302 False High Sequest HT (A2) Unambiguous EEEAAEESDDDMGFGLFD [K].eEEAAEEsDDDMGFGLFD.[-] N-Term(TMT6plex); S8(Phospho) 2 P05319 P10622; P05319 0 3 0.7606 0 1 1 772.29991 2314.88516 2314.8784 2.92 0.00225 341376.625 CID (Collision Induced Dissociation) MS2 57.93543 60 36.1 60.0 143.6038 49003 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 8.9 32.0 43.5 12.3 25.5 70.5 13.7 34.1 75.3 44.8 Excluded by Method 3.55 1 0.0003003 0.0001504 False High Sequest HT (A2) Unambiguous YAAAAPAATSAASGDAAPAEEAAAEEEEESDDDMGFGLFD [K].yAAAAPAATSAASGDAAPAEEAAAEEEEEsDDDmGFGLFD.[-] N-Term(TMT6plex); S30(Phospho); M34(Oxidation) 1 P05317 P05317 0 4 0.3605 0 1 1 1062.19431 4245.75543 4245.73286 5.31 0.00564 440991.15625 CID (Collision Induced Dissociation) MS2 0.0 60 13.8 60.0 142.9963 48795 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 11.2 26.1 2.9 10.8 27.8 2.9 15.9 27.2 13.4 Excluded by Method 4.3 1 0.0003003 0.0002708 False High Sequest HT (A2) Unambiguous EEEAAEESDDDMGFGLFD [K].eEEAAEEsDDDMGFGLFD.[-] N-Term(TMT6plex); S8(Phospho) 2 P05319 P10622; P05319 0 3 0.7984 0 1 1 772.29917 2314.88297 2314.8784 1.97 0.00152 540574.6875 CID (Collision Induced Dissociation) MS2 0.0 60 18.5 60.0 142.6328 48666 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 6.6 15.8 26.0 2.8 16.7 33.8 8.1 17.6 37.4 20.1 Excluded by Method 3.82 1 0.0003003 0.000171 False High Sequest HT (A2) Unambiguous EEEAAEESDDDMGFGLFD [K].eEEAAEEsDDDMGFGLFD.[-] N-Term(TMT6plex); S8(Phospho) 2 P05319 P10622; P05319 0 3 0.797 0 1 1 772.29801 2314.87949 2314.8784 0.47 0.00036 336754.1875 CID (Collision Induced Dissociation) MS2 6.910796 70 24.2 60.0 141.5539 48296 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 3.8 25.3 34.1 4.0 16.8 50.2 8.9 21.1 55.0 22.9 3.99 1 0.0003003 2.693e-05 False High Sequest HT (A2) Unambiguous YAAAAPAATSAASGDAAPAEEAAAEEEEESDDDMGFGLFD [K].yAAAAPAATSAASGDAAPAEEAAAEEEEEsDDDmGFGLFD.[-] N-Term(TMT6plex); S30(Phospho); M34(Oxidation) 1 P05317 P05317 0 4 0.2045 0 1 1 1062.19187 4245.74566 4245.73286 3.01 0.0032 577197.0 CID (Collision Induced Dissociation) MS2 0.0 50 13.8 60.0 141.503 48278 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 2.4 12.3 22.6 2.8 12.2 26.5 5.9 14.1 23.6 15.5 Excluded by Method 4.89 1 0.0003003 0.0002116 False High Sequest HT (A2) Unambiguous EEEAAEESDDDMGFGLFD [K].eEEAAEEsDDDMGFGLFD.[-] N-Term(TMT6plex); S8(Phospho) 2 P05319 P10622; P05319 0 3 0.7971 0 1 1 772.29795 2314.87931 2314.8784 0.39 0.0003 337902.96875 CID (Collision Induced Dissociation) MS2 0.0 60 27.3 60.0 140.4228 47907 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 10.1 20.8 36.8 9.1 22.5 50.2 14.2 28.2 52.7 28.4 Excluded by Method 4.19 1 0.0003003 0.000273 False High Sequest HT (A2) Unambiguous YAAAAPAATSAASGDAAPAEEAAAEEEEESDDDMGFGLFD [K].yAAAAPAATSAASGDAAPAEEAAAEEEEEsDDDmGFGLFD.[-] N-Term(TMT6plex); S30(Phospho); M34(Oxidation) 1 P05317 P05317 0 4 0.349 0 1 1 1062.19065 4245.74078 4245.73286 1.86 0.00198 489795.5625 CID (Collision Induced Dissociation) MS2 7.458574 70 17.5 60.0 140.2754 47857 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 2.4 18.6 24.6 4.2 11.3 37.5 9.3 17.2 34.4 15.6 4.67 1 0.0003003 0.000117 False High Sequest HT (A2) Unambiguous EEEAAEESDDDMGFGLFD [K].eEEAAEEsDDDmGFGLFD.[-] N-Term(TMT6plex); S8(Phospho); M12(Oxidation) 2 P05319 P10622; P05319 0 2 0.7871 0 1 1 1165.94192 2330.87656 2330.87332 1.39 0.00162 12974660.0 CID (Collision Induced Dissociation) MS2 52.46475 70 41.5 8.398 129.0496 43651 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 12.8 33.6 53.9 11.7 34.0 83.8 14.6 43.6 76.8 49.8 4.65 1 0.0003003 0.0003965 False High Sequest HT (A2) Unambiguous AREAAAGIQWSEEETEDEEEEK [K].aREAAAGIQWsEEEtEDEEEEk.[E] N-Term(TMT6plex); S11(Phospho); T15(Phospho); K22(TMT6plex) 4 P46087 P46087-3; P46087-2; P46087; P46087-4 1 3 0.6084 0 1 1 1052.12566 3154.36244 3154.3543 2.58 0.00271 428919.875 CID (Collision Induced Dissociation) MS2 16.72708 30 77.7 60.0 82.0105 25951 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 81.1 72.0 62.2 85.9 68.4 77.2 92.9 76.7 89.4 71.6 Excluded by Method 4.98 1 0.0003003 4.592e-05 False High Sequest HT (A2) Unambiguous EYVPLDNAEQSTSSSQETK [K].eYVPLDNAEQSTsSSQETk.[E] N-Term(TMT6plex); S13(Phospho); K19(TMT6plex) 1 P33322 P33322 0 3 0.013 0 1 1 884.4227 2651.25356 2651.24896 1.74 0.00153 809128.0625 CID (Collision Induced Dissociation) MS2 7.686621 70 62.7 60.0 81.8246 25881 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 19.5 55.6 90.6 21.5 51.8 111.5 26.0 62.3 121.1 66.7 5.4 1 0.0003003 8.693e-05 False High Sequest HT (A2) Unambiguous SPSDSGYSYETIGK [K].sPSDSGYSYETIGk.[T] N-Term(TMT6plex); S1(Phospho); K14(TMT6plex) 1 P46821 P46821 0 2 0.1132 0 1 1 1014.98204 2028.9568 2028.95674 0.03 3e-05 881174.875 CID (Collision Induced Dissociation) MS2 0.0 50 93.9 60.0 81.6788 25826 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 101.0 93.7 76.4 88.0 97.5 89.8 113.4 102.9 89.7 86.2 Excluded by Method 4.86 1 0.0003003 2.014e-07 False High Sequest HT (A2) Unambiguous ADDEEDLSDENIQPELRGQ [K].aDDEEDLsDENIQPELRGQ.[-] N-Term(TMT6plex); S8(Phospho) 1 P14164 P14164 1 3 0.4881 0 1 1 828.03252 2482.08301 2482.082 0.41 0.00034 2187067.25 CID (Collision Induced Dissociation) MS2 0.0 50 73.8 60.0 81.6725 25823 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 19.0 56.9 114.8 22.5 60.2 134.2 22.9 77.7 157.9 72.4 Excluded by Method 6.31 1 0.0003003 8.748e-08 False High Sequest HT (A2) Unambiguous DFELEETDEEK [K].dFELEETDEEk.[A] N-Term(TMT6plex); K11(TMT6plex) 2 P15108 P02829; P15108 0 3 0.4006 0 1 1 614.64023 1841.90613 1841.90568 0.25 0.00015 3791603.5 CID (Collision Induced Dissociation) MS2 10.07554 80 61.6 31.772 81.6548 25816 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 24.4 42.9 77.9 27.0 54.4 113.0 31.8 62.6 117.1 65.2 3.62 1 0.0003003 0.0001108 False High Sequest HT (A2) Unambiguous ESEQESEEEILAQK [K].eSEQEsEEEILAQk.[K] N-Term(TMT6plex); S6(Phospho); K14(TMT6plex) 1 Q9BW71 Q9BW71 0 3 0.0189 0 1 1 729.68573 2187.04263 2187.04701 -2.0 -0.00146 4307921.5 CID (Collision Induced Dissociation) MS2 3.358987 20 132.2 18.246 81.776 25862 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 148.5 131.8 109.7 138.3 122.0 115.3 159.2 128.3 127.2 141.2 Excluded by Method 4.23 1 0.0003003 8.692e-05 False High Sequest HT (A2) Unambiguous LVEGLANDPENK [K].lVEGLANDPENk.[V] N-Term(TMT6plex); K12(TMT6plex) 1 P48589 P48589 0 3 0.2396 0 1 1 586.33271 1756.98358 1756.98453 -0.54 -0.00032 857186.5 CID (Collision Induced Dissociation) MS2 3.671168 50 66.8 60.0 82.7647 26239 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 23.6 56.6 89.5 29.0 53.7 132.5 31.3 66.8 122.5 62.7 Excluded by Method 4.55 1 0.0003003 4.181e-05 False High Sequest HT (A2) Unambiguous QASASYDSEEEEEGLPMSYDEK [K].qASAsYDSEEEEEGLPmSYDEk.[R] N-Term(TMT6plex); S5(Phospho); M17(Oxidation); K22(TMT6plex) 1 Q15059 Q15059 0 3 0.0286 0 1 1 1016.77171 3048.30057 3048.29571 1.6 0.00162 528108.8125 CID (Collision Induced Dissociation) MS2 0.0 30 18.0 60.0 82.4323 26111 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 15.4 19.1 15.5 13.8 22.5 16.9 20.4 22.3 18.5 15.9 Excluded by Method 3.85 1 0.0003003 0.000303 False High Sequest HT (A2) Unambiguous LNNFNDVSSYNENK [K].lNNFNDVSSYNENk.[S] N-Term(TMT6plex); K14(TMT6plex) 1 P32582 P32582 0 3 0.0669 0 1 1 706.02945 2116.0738 2116.07112 1.27 0.00089 2031941.5 CID (Collision Induced Dissociation) MS2 0.0 70 73.6 60.0 82.6542 26196 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 63.7 80.4 62.7 70.6 69.1 83.6 71.3 81.1 84.8 68.3 2.99 1 0.0003003 0.0002478 False High Sequest HT (A2) Unambiguous QREMLMEDVGSEEEQEEEDEAPFQEK [K].qREmLmEDVGsEEEQEEEDEAPFQEk.[D] N-Term(TMT6plex); M4(Oxidation); M6(Oxidation); S11(Phospho); K26(TMT6plex) 2 Q9H1E3 Q9H1E3; Q9H1E3-2 1 3 0.6238 0 1 1 1237.87224 3711.60217 3711.59672 1.47 0.00182 1659434.375 CID (Collision Induced Dissociation) MS2 0.0 50 86.4 60.0 83.2688 26427 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 87.7 91.4 69.4 81.4 80.5 87.1 97.6 89.0 97.8 82.1 Excluded by Method 5.13 1 0.0003003 3.73e-07 False High Sequest HT (A2) Unambiguous LDESDENEEALAK [K].lDEsDENEEALAk.[A] N-Term(TMT6plex); S4(Phospho); K13(TMT6plex) 1 Q08492 Q08492 0 3 0.5533 0 1 1 667.65383 2000.94694 2000.94657 0.18 0.00012 2195982.5 CID (Collision Induced Dissociation) MS2 1.993169 20 155.5 50.123 81.2418 25660 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 32.3 121.6 241.1 51.7 121.3 281.7 56.5 150.0 326.7 172.0 Excluded by Method 4.41 1 0.0003003 0.0001815 False High Sequest HT (A2) Unambiguous DDFESEEEDVK [K].dDFEsEEEDVk.[S] N-Term(TMT6plex); S5(Phospho); K11(TMT6plex) 3 Q7Z6E9 Q7Z6E9-4; Q7Z6E9-2; Q7Z6E9 0 2 0.603 0 1 1 940.41646 1879.82565 1879.82506 0.32 0.0003 9125182.0 CID (Collision Induced Dissociation) MS2 10.1205 40 96.7 21.713 79.0453 24839 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 94.1 90.1 72.6 111.4 88.4 93.3 116.3 108.6 95.1 97.0 Excluded by Method 4.66 1 0.0003003 0.0001008 False High Sequest HT (A2) Unambiguous NLENDTTSSPTQDLDEK [K].nLENDTTSsPTQDLDEk.[S] N-Term(TMT6plex); S9(Phospho); K17(TMT6plex) 1 Q04279 Q04279 0 3 0.1168 0 1 1 815.71955 2445.14409 2445.14343 0.27 0.00022 2417955.25 CID (Collision Induced Dissociation) MS2 2.342654 70 71.4 46.097 78.3147 24564 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 14.8 62.3 103.6 24.3 54.6 124.6 24.3 73.2 148.6 83.8 4.28 1 0.0003003 5.408e-05 False High Sequest HT (A2) Unambiguous ATLEEAEGESGVEDDAATGSSNK [K].aTLEEAEGEsGVEDDAATGsSNk.[L] N-Term(TMT6plex); S10(Phospho); S20(Phospho); K23(TMT6plex) 1 P21304 P21304 0 3 0.0999 0 1 1 962.41766 2885.23841 2885.23787 0.19 0.00018 616766.9375 CID (Collision Induced Dissociation) MS2 0.0 80 67.2 60.0 80.8474 25509 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 20.0 61.2 100.6 18.5 53.5 127.3 26.7 57.6 135.3 70.8 6.81 1 0.0003003 1.067e-07 False High Sequest HT (A2) Unambiguous EAPAEGEAAEPGSPTAAEGEAASAASSTSSPK [K].eAPAEGEAAEPGSPtAAEGEAASAASStSSPk.[A] N-Term(TMT6plex); T15(Phospho); T28(Phospho); K32(TMT6plex) 1 P29966 P29966 0 3 0.0113 0 1 1 1178.52503 3533.56054 3533.561 -0.13 -0.00015 473780.25 CID (Collision Induced Dissociation) MS2 37.8655 60 32.8 60.0 80.7474 25469 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 33.7 25.7 24.7 36.5 39.0 32.0 38.4 30.4 35.3 32.3 Excluded by Method 4.42 1 0.0003003 0.0001095 False High Sequest HT (A2) Unambiguous QSFDDNDSEELEDK [K].qSFDDNDsEELEDk.[D] N-Term(TMT6plex); S8(Phospho); K14(TMT6plex) 1 O60841 O60841 0 3 0.0789 0 1 1 736.99163 2208.96033 2208.95859 0.79 0.00058 760009.4375 CID (Collision Induced Dissociation) MS2 19.29181 50 62.0 60.0 79.5398 25022 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 59.9 79.4 44.6 68.8 53.6 58.1 69.1 58.6 69.7 58.5 Excluded by Method 3.55 1 0.0003003 0.0004018 False High Sequest HT (A2) Unambiguous LFEDDDSNEK [K].lFEDDDsNEk.[L] N-Term(TMT6plex); S7(Phospho); K10(TMT6plex) 1 O43719 O43719 0 2 0.4931 0 1 1 875.40355 1749.79982 1749.79845 0.79 0.00069 1981197.75 CID (Collision Induced Dissociation) MS2 0.0 60 77.5 60.0 87.277 27928 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 69.2 80.9 54.0 88.7 76.9 72.8 97.3 78.0 86.4 71.0 Excluded by Method 4.36 1 0.0003003 2.783e-05 False High Sequest HT (A2) Unambiguous EEPLSEEEPCTSTAIASPEK [K].eEPLsEEEPcTSTAIASPEk.[K] N-Term(TMT6plex); S5(Phospho); C10(Carbamidomethyl); K20(TMT6plex) 1 Q9Y2X3 Q9Y2X3 0 3 0.2214 0 1 1 914.76647 2742.28487 2742.28329 0.57 0.00052 8453058.0 CID (Collision Induced Dissociation) MS2 1.265377 70 146.4 16.689 86.8269 27754 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 155.9 150.1 123.0 150.6 141.8 131.5 175.3 154.3 142.5 139.1 5.6 1 0.0003003 1.515e-05 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVssDEEF.[-] N-Term(TMT6plex); S8(Phospho); S9(Phospho) 1 P16521 P16521 0 2 0.1448 0 1 1 925.35501 1849.70275 1849.70195 0.43 0.0004 850288.0 CID (Collision Induced Dissociation) MS2 0.0 40 84.2 60.0 148.9339 50899 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 15.3 65.4 143.1 22.8 66.9 160.5 27.4 74.8 179.9 86.2 Excluded by Method 3.73 1 0.0003003 0.0003552 False High Sequest HT (A2) Unambiguous VFDDESDEKEDEEYADEK [K].vFDDEsDEkEDEEYADEk.[G] N-Term(TMT6plex); S6(Phospho); K9(TMT6plex); K18(TMT6plex) 1 O43719 O43719 1 4 0.4274 0 1 1 740.58647 2959.32405 2959.32247 0.54 0.0004 1485601.5 CID (Collision Induced Dissociation) MS2 0.0 20 47.3 60.0 86.8285 27755 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 49.4 44.0 35.1 69.7 41.1 42.9 57.2 44.6 43.7 44.9 Excluded by Method 4.68 1 0.0003003 0.0001634 False High Sequest HT (A2) Unambiguous KPTPLPSLK [K].kPtPLPsLk.[D] N-Term(TMT6plex); K1(TMT6plex); T3(Phospho); S7(Phospho); K9(TMT6plex) 1 P06105 P06105 0 3 0.5221 0 1 1 610.01676 1828.03573 1828.03535 0.21 0.00013 1122007.125 CID (Collision Induced Dissociation) MS2 0.0 50 374.9 60.0 86.2901 27553 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 96.7 297.3 572.7 137.4 302.5 685.9 134.7 368.5 746.3 406.7 Excluded by Method 3.62 1 0.0003003 0.0001428 False High Sequest HT (A2) Unambiguous RSIFASPESVTGK [K].rSIFAsPESVTGk.[V] N-Term(TMT6plex); S6(Phospho); K13(TMT6plex) 1 O75940 O75940 1 3 0.1609 0 1 1 639.67993 1917.02523 1917.0247 0.28 0.00018 2049995.625 CID (Collision Induced Dissociation) MS2 4.149837 50 65.8 56.728 87.5181 28015 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 68.7 65.9 58.4 63.9 64.8 71.1 70.5 66.4 62.8 65.9 Excluded by Method 4.04 1 0.0003003 7.582e-05 False High Sequest HT (A2) Unambiguous GNAGGSNDDFTAPISATAK [K].gNAGGsNDDFTAPISATAk.[M] N-Term(TMT6plex); S6(Phospho); K19(TMT6plex) 1 P32578 P32578 0 3 0.3119 0 1 1 778.04553 2332.12203 2332.12224 -0.09 -7e-05 5076517.0 CID (Collision Induced Dissociation) MS2 0.6244891 50 47.0 27.491 88.4653 28363 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 14.6 41.6 67.6 14.3 38.6 95.4 16.8 37.7 94.5 48.7 Excluded by Method 4.2 1 0.0003003 0.0002451 False High Sequest HT (A2) Unambiguous YDEYFNEYNNNSN [K].yDEYFNEYNNNSN.[-] N-Term(TMT6plex) 1 P32776 P32776 0 2 0.7143 0 1 1 957.90399 1914.8007 1914.79797 1.43 0.00137 523820.125 CID (Collision Induced Dissociation) MS2 20.81597 80 78.5 60.0 88.0947 28225 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 22.8 57.0 126.1 27.1 69.4 160.5 27.2 70.7 153.6 70.5 3.78 1 0.0003003 1.319e-05 False High Sequest HT (A2) Unambiguous GLQTVVVEEDA [K].gLQTVVVEEDA.[-] N-Term(TMT6plex) 2 P0C2H8 P0C2H8; P0C2H9 0 2 0.6619 0 1 1 694.87712 1388.74696 1388.74704 -0.06 -4e-05 2798182.0 CID (Collision Induced Dissociation) MS2 4.000221 90 220.3 60.0 88.0604 28211 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 54.1 181.6 367.7 56.0 167.0 430.6 76.1 204.9 430.0 235.0 2.81 1 0.0003003 0.0001854 False High Sequest HT (A2) Unambiguous LFDEEEDSSEK [K].lFDEEEDSsEk.[L] N-Term(TMT6plex); S9(Phospho); K11(TMT6plex) 1 O43719 O43719 0 2 0.0175 0 1 1 933.42642 1865.84557 1865.84579 -0.12 -0.00011 3143826.0 CID (Collision Induced Dissociation) MS2 10.10454 20 322.8 60.0 88.0447 28205 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 339.7 317.0 238.9 389.2 301.5 286.3 391.0 319.5 350.4 294.9 Excluded by Method 4.56 1 0.0003003 1.754e-05 False High Sequest HT (A2) Unambiguous VDSPDDPFMTK [K].vDsPDDPFmTk.[S] N-Term(TMT6plex); S3(Phospho); M9(Oxidation); K11(TMT6plex) 1 P19659 P19659 0 3 0.5148 0 1 1 602.61937 1805.84356 1805.84329 0.15 9e-05 1795752.125 CID (Collision Induced Dissociation) MS2 0.0 80 110.9 60.0 87.8088 28119 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 30.1 88.6 153.8 33.7 89.3 221.6 48.4 104.2 222.0 117.3 3.71 1 0.0003003 0.0005555 False High Sequest HT (A2) Unambiguous LFEDDDSNEK [K].lFEDDDsNEk.[L] N-Term(TMT6plex); S7(Phospho); K10(TMT6plex) 1 O43719 O43719 0 3 0.4026 0 1 1 583.938 1749.79945 1749.79845 0.57 0.00033 2201802.75 CID (Collision Induced Dissociation) MS2 4.31793 60 225.0 46.933 86.086 27475 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 235.5 227.6 177.1 264.9 215.6 198.1 280.3 245.7 207.4 197.7 Excluded by Method 3.8 1 0.0003003 6.982e-06 False High Sequest HT (A2) Unambiguous IPSPEFNSNNEK [K].iPsPEFNSNNEk.[E] N-Term(TMT6plex); S3(Phospho); K12(TMT6plex) 1 P38250 P38250 0 3 0.3779 0 1 1 638.65192 1913.94119 1913.94103 0.09 5e-05 1347542.5 CID (Collision Induced Dissociation) MS2 0.0 40 51.0 57.706 84.509 26884 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 16.0 43.7 75.2 19.4 49.5 103.1 15.9 47.3 93.7 46.7 Excluded by Method 5.16 1 0.0003003 6.099e-05 False High Sequest HT (A2) Unambiguous SNVPESINEDISK [K].sNVPEsINEDISk.[T] N-Term(TMT6plex); S6(Phospho); K13(TMT6plex) 1 Q08412 Q08412 0 3 0.1047 0 1 1 657.33432 1969.98842 1969.98837 0.02 2e-05 1807843.0 CID (Collision Induced Dissociation) MS2 6.379102 30 136.4 46.607 84.1892 26765 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 33.4 105.3 203.2 41.7 111.8 258.9 39.9 139.9 285.4 144.8 Excluded by Method 4.3 1 0.0003003 0.0001699 False High Sequest HT (A2) Unambiguous ENENADDDMDSLFGDEDEDEK [K].eNENADDDmDsLFGDEDEDEk.[Q] N-Term(TMT6plex); M9(Oxidation); S11(Phospho); K21(TMT6plex) 1 P38915 P38915 0 3 0.5355 0 1 1 996.06403 2986.17755 2986.1709 2.23 0.00221 221047.671875 CID (Collision Induced Dissociation) MS2 0.0 50 22.7 60.0 84.1417 26748 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 3.9 18.0 30.7 9.7 19.5 43.0 10.5 23.6 43.3 24.8 Excluded by Method 4.22 1 0.0003003 3.28e-05 False High Sequest HT (A2) Unambiguous EESVGSDDDATAANQQVYLK [K].eESVGsDDDATAANQQVYLk.[T] N-Term(TMT6plex); S6(Phospho); K20(TMT6plex) 1 Q03305 Q03305 0 3 0.0212 0 1 1 893.42544 2678.26176 2678.25986 0.71 0.00064 3594912.25 CID (Collision Induced Dissociation) MS2 58.87191 90 59.0 48.792 84.3472 26823 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 22.5 45.3 91.0 21.8 43.4 103.5 26.4 55.3 117.8 63.2 6.12 1 0.0003003 1.397e-07 False High Sequest HT (A2) Unambiguous SPGASNFSTLPK [K].sPGASNFSTLPk.[I] N-Term(TMT6plex); S1(Phospho); K12(TMT6plex) 1 Q9Y4E8 Q9Y4E8 0 2 0.3371 0 1 1 872.45824 1743.90921 1743.90827 0.54 0.00047 2020967.375 CID (Collision Induced Dissociation) MS2 0.0 40 36.0 56.92 85.114 27112 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 36.5 33.9 31.3 33.1 39.2 32.3 43.7 35.8 37.5 37.0 Excluded by Method 3.56 1 0.0003003 0.0004141 False High Sequest HT (A2) Unambiguous VLDEEGSEREFDEDSDEK [K].vLDEEGsEREFDEDsDEk.[E] N-Term(TMT6plex); S7(Phospho); S15(Phospho); K18(TMT6plex) 1 O43719 O43719 1 3 0.598 0 1 1 916.05273 2746.14363 2746.14218 0.53 0.00048 2092530.375 CID (Collision Induced Dissociation) MS2 33.11043 20 147.3 46.708 85.1163 27113 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 150.2 138.2 111.6 161.5 139.4 147.8 178.6 154.0 148.7 143.3 Excluded by Method 4.9 1 0.0003003 0.0003794 False High Sequest HT (A2) Unambiguous GPTTGEGALDLSDVHSPPKSPEGK [K].gPTTGEGALDLSDVHsPPksPEGk.[T] N-Term(TMT6plex); S16(Phospho); K19(TMT6plex); S20(Phospho); K24(TMT6plex) 2 O95684 O95684-2; O95684 1 4 0.4895 0 1 1 806.65346 3223.59201 3223.58922 0.87 0.0007 950319.125 CID (Collision Induced Dissociation) MS2 0.0 50 72.3 60.0 85.9012 27407 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 69.1 72.3 63.2 79.1 63.7 72.9 93.0 70.0 75.2 64.2 Excluded by Method 6.68 1 0.0003003 7.362e-06 False High Sequest HT (A2) Unambiguous KPYRIESDEEEDFENVGK [K].kPYRIEsDEEEDFENVGk.[V] N-Term(TMT6plex); K1(TMT6plex); S7(Phospho); K18(TMT6plex) 3 Q96T23 Q96T23; Q96T23-3; Q96T23-2 1 4 0.3731 0 1 1 738.62212 2951.46664 2951.46426 0.81 0.0006 3586108.0 CID (Collision Induced Dissociation) MS2 0.0 10 285.0 43.039 85.895 27404 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 311.7 283.5 232.6 310.2 290.3 251.4 329.8 296.2 270.3 274.1 Excluded by Method 4.02 1 0.0003003 7.508e-05 False High Sequest HT (A2) Unambiguous LPRTQPDGTSVPGEPASPISQRLPPK [K].lPRTQPDGTSVPGEPAsPISQRLPPk.[V] N-Term(TMT6plex); S17(Phospho); K26(TMT6plex) 3 Q14980 Q14980-5; Q14980-2; Q14980 2 4 0.134 0 1 1 816.69373 3263.75309 3263.75535 -0.69 -0.00056 1406585.625 CID (Collision Induced Dissociation) MS2 3.875062 70 84.9 60.0 85.7104 27334 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 80.6 76.4 71.9 76.6 81.1 96.9 91.5 93.8 89.3 91.0 5.67 1 0.0003003 6.391e-05 False High Sequest HT (A2) Unambiguous QETVADFTPK [K].qETVADFtPk.[K] N-Term(TMT6plex); T8(Phospho); K10(TMT6plex) 3 O75400 O75400-2; O75400-3; O75400 0 3 0.5257 0 1 1 558.62321 1673.85509 1673.85517 -0.05 -3e-05 3465501.75 CID (Collision Induced Dissociation) MS2 3.922746 80 129.6 39.637 77.7514 24350 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 133.8 129.5 107.4 129.8 134.9 126.6 139.3 143.8 132.9 118.1 4.87 1 0.0003003 1.482e-06 False High Sequest HT (A2) Unambiguous EMLASDDEEDVSSK [K].emLAsDDEEDVSSk.[V] N-Term(TMT6plex); M2(Oxidation); S5(Phospho); K14(TMT6plex) 4 Q0ZGT2 Q0ZGT2-2; Q0ZGT2-4; Q0ZGT2; Q0ZGT2-3 0 2 0.5446 0 1 1 1054.97258 2108.93789 2108.93468 1.52 0.0016 153831.8125 CID (Collision Induced Dissociation) MS2 0.0 20 96.5 60.0 65.2886 19667 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 90.8 93.5 72.2 97.3 96.5 95.3 111.9 108.0 96.0 103.3 Excluded by Method 4.04 1 0.0003003 1.74e-05 False High Sequest HT (A2) Unambiguous QSFDDNDSEELEDK [K].qSFDDNDsEELEDk.[D] N-Term(TMT6plex); S8(Phospho); K14(TMT6plex) 1 O60841 O60841 0 2 0.4783 0 1 1 1104.98265 2208.95802 2208.95859 -0.26 -0.00028 998362.0625 CID (Collision Induced Dissociation) MS2 0.0 30 38.3 60.0 77.6466 24312 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 33.2 36.0 25.5 48.7 39.8 40.9 38.1 40.3 42.3 38.0 Excluded by Method 3.91 1 0.0003003 1.293e-05 False High Sequest HT (A2) Unambiguous EMLASDDEEDVSSK [K].eMLAsDDEEDVSSk.[V] N-Term(TMT6plex); S5(Phospho); K14(TMT6plex) 4 Q0ZGT2 Q0ZGT2-2; Q0ZGT2-4; Q0ZGT2; Q0ZGT2-3 0 3 0.6206 0 1 1 698.31767 2092.93845 2092.93977 -0.63 -0.00044 3165342.5 CID (Collision Induced Dissociation) MS2 0.8326551 40 111.0 44.821 77.4617 24243 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 113.4 110.5 86.9 113.8 96.5 118.1 126.6 120.9 109.0 114.0 Excluded by Method 4.56 1 0.0003003 7.264e-05 False High Sequest HT (A2) Unambiguous VNNEEAISEDDGIEEEHIHSEK [K].vNNEEAIsEDDGIEEEHIHSEk.[S] N-Term(TMT6plex); S8(Phospho); K22(TMT6plex) 1 P39993 P39993 0 4 0.4957 0 1 1 766.10505 3061.39838 3061.40395 -1.82 -0.00139 562979.5 CID (Collision Induced Dissociation) MS2 15.05969 60 78.1 60.0 69.2468 21145 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 22.2 72.2 111.0 26.3 67.3 144.4 30.0 72.4 151.8 83.7 Excluded by Method 6.94 1 0.0003003 1.773e-05 False High Sequest HT (A2) Unambiguous SSSPSASLTENEVK [K].sSSPsASLTENEVk.[-] N-Term(TMT6plex); S5(Phospho); K14(TMT6plex) 1 O96028-5 O96028-5 0 3 0.0545 0 1 1 658.66727 1973.98725 1973.98329 2.01 0.00132 1062310.375 CID (Collision Induced Dissociation) MS2 0.0 40 79.0 60.0 69.2358 21141 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 82.9 87.5 58.3 80.9 74.6 80.0 83.1 84.2 79.1 79.5 Excluded by Method 4.77 1 0.0003003 0.0003363 False High Sequest HT (A2) Unambiguous YNLDASEEEDSNK [K].yNLDAsEEEDSNk.[K] N-Term(TMT6plex); S6(Phospho); K13(TMT6plex) 2 O95218 O95218-2; O95218 0 2 0.2814 0 1 1 1026.46383 2051.92039 2051.92108 -0.34 -0.00035 1246540.25 CID (Collision Induced Dissociation) MS2 0.0 40 201.4 60.0 69.688 21312 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 197.0 191.9 176.4 216.4 192.1 190.5 222.7 221.3 216.4 189.4 Excluded by Method 5.33 1 0.0003003 3.042e-06 False High Sequest HT (A2) Unambiguous EVEDKESEGEEEDEDEDLSK [K].eVEDkEsEGEEEDEDEDLSk.[Y] N-Term(TMT6plex); K5(TMT6plex); S7(Phospho); K20(TMT6plex) 2 O95218 O95218-2; O95218 1 3 0.5208 0 1 1 1036.4676 3107.38826 3107.392 -1.2 -0.00125 3414345.25 CID (Collision Induced Dissociation) MS2 32.98585 50 111.1 60.0 68.9216 21021 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 124.0 118.1 78.8 141.2 112.4 88.0 140.4 110.9 102.4 94.9 Excluded by Method 6.72 1 0.0003003 7.111e-08 False High Sequest HT (A2) Unambiguous ENETDEENTEVMIK [K].eNEtDEENTEVmIk.[G] N-Term(TMT6plex); T4(Phospho); M12(Oxidation); K14(TMT6plex) 1 Q9HAU5 Q9HAU5 0 3 0.2384 0 1 1 745.6768 2235.01584 2235.014 0.82 0.00061 1355676.25 CID (Collision Induced Dissociation) MS2 0.0 70 65.4 60.0 69.9295 21403 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 56.0 61.3 56.3 74.2 67.9 68.1 72.5 72.8 69.0 55.8 5.41 1 0.0003003 6.434e-07 False High Sequest HT (A2) Unambiguous SSTSFANIQENSN [K].sSTsFANIQENSN.[-] N-Term(TMT6plex); S4(Phospho) 1 Q86WC4 Q86WC4 0 2 0.0284 0 1 1 854.37489 1707.74251 1707.74244 0.04 3e-05 3827228.25 CID (Collision Induced Dissociation) MS2 0.0 80 128.4 60.0 71.7321 22079 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 125.5 122.0 115.9 122.5 119.0 124.6 148.9 139.3 132.5 133.5 4.22 1 0.0003003 1.23e-06 False High Sequest HT (A2) Unambiguous RLLGDSDSEEEQK [K].rLLGDsDsEEEQk.[E] N-Term(TMT6plex); S6(Phospho); S8(Phospho); K13(TMT6plex) 1 Q9BW71 Q9BW71 1 3 0.5922 0 1 1 708.66018 2123.966 2123.96633 -0.16 -0.00011 2403990.25 CID (Collision Induced Dissociation) MS2 0.0 40 138.3 60.0 71.5778 22019 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 149.8 140.0 106.8 151.8 125.2 134.7 161.2 148.8 132.0 132.9 Excluded by Method 5.1 1 0.0003003 1.057e-05 False High Sequest HT (A2) Unambiguous EMEHNTVCAAGTSPVGEIGEEK [K].emEHNTVcAAGTsPVGEIGEEk.[I] N-Term(TMT6plex); M2(Oxidation); C8(Carbamidomethyl); S13(Phospho); K22(TMT6plex) 9 P18583 P18583; P18583-4; P18583-6; P18583-5; P18583-10; P18583-9; P18583-2; P18583-3; P18583-7 0 3 0.0579 0 1 1 967.11369 2899.32653 2899.32551 0.35 0.00034 1605897.125 CID (Collision Induced Dissociation) MS2 15.25978 20 166.5 60.0 71.4771 21982 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 168.3 157.4 126.8 193.6 162.9 148.5 194.1 186.5 157.2 169.7 Excluded by Method 3.8 1 0.0003003 0.0003063 False High Sequest HT (A2) Unambiguous LEEVDEEEEEK [K].lEEVDEEEEEk.[K] N-Term(TMT6plex); K11(TMT6plex) 1 P15108 P15108 0 2 0.4771 0 1 1 918.4616 1835.91592 1835.91624 -0.17 -0.00016 669868.4375 CID (Collision Induced Dissociation) MS2 0.0 90 18.2 60.0 71.099 21842 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 7.0 14.2 24.4 7.8 15.4 32.7 6.8 18.6 37.9 17.1 3.71 1 0.0003003 1.903e-07 False High Sequest HT (A2) Unambiguous EDSAPVAAGESESSL [K].eDSAPVAAGESEsSL.[-] N-Term(TMT6plex); S13(Phospho) 1 P25567 P25567 0 2 0.2208 0 1 1 879.38792 1757.76856 1757.76799 0.33 0.00029 2401788.5 CID (Collision Induced Dissociation) MS2 0.0 90 148.3 60.0 71.1268 21852 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 33.7 114.6 242.9 40.4 123.0 293.5 50.4 135.0 296.7 152.3 4.71 1 0.0003003 1.281e-06 False High Sequest HT (A2) Unambiguous GEPAAAAAPEAGASPVEK [K].gEPAAAAAPEAGAsPVEk.[E] N-Term(TMT6plex); S14(Phospho); K18(TMT6plex) 1 P29966 P29966 0 3 0.5758 0 1 1 721.03657 2161.09516 2161.09424 0.43 0.00031 1360247.125 CID (Collision Induced Dissociation) MS2 8.225119 40 260.7 60.0 65.6523 19803 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 281.7 245.3 219.5 263.1 244.9 257.0 288.3 287.2 254.7 265.0 Excluded by Method 6.86 1 0.0003003 8.359e-08 False High Sequest HT (A2) Unambiguous FSPGAPGGSGSQPNQK [K].fsPGAPGGSGSQPNQk.[L] N-Term(TMT6plex); S2(Phospho); K16(TMT6plex) 2 Q15942 Q15942; Q15942-2 0 3 0.2786 0 1 1 685.34222 2054.01211 2054.01084 0.62 0.00042 9768020.0 CID (Collision Induced Dissociation) MS2 6.777282 60 152.6 35.683 65.5918 19779 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 151.5 133.9 127.0 148.1 142.9 147.1 163.7 166.5 174.7 170.1 Excluded by Method 4.81 1 0.0003003 5.763e-06 False High Sequest HT (A2) Unambiguous AGLDNVDAESK [K].aGLDNVDAEsk.[E] N-Term(TMT6plex); S10(Phospho); K11(TMT6plex) 1 P09064 P09064 0 3 0.5099 0 1 1 552.9464 1656.82466 1656.8246 0.03 2e-05 1828203.0 CID (Collision Induced Dissociation) MS2 25.18517 90 199.4 60.0 66.6668 20180 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 48.9 172.9 293.8 63.6 165.7 357.6 77.6 189.6 410.8 213.5 4.53 1 0.0003003 7.738e-05 False High Sequest HT (A2) Unambiguous HPNYSPPGSPIEK [K].hPNYsPPGsPIEk.[Y] N-Term(TMT6plex); S5(Phospho); S9(Phospho); K13(TMT6plex) 3 Q9UHF7 Q9UHF7-3; Q9UHF7-2; Q9UHF7 0 3 0.0097 0 1 1 680.99146 2040.95982 2040.95973 0.04 3e-05 1010906.625 CID (Collision Induced Dissociation) MS2 18.10723 60 160.2 60.0 67.5822 20518 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 155.5 162.2 131.0 157.8 150.5 170.6 195.2 173.7 159.1 146.8 Excluded by Method 5.18 1 0.0003003 2.573e-05 False High Sequest HT (A2) Unambiguous VSGTLDTPEK [K].vSGTLDtPEk.[T] N-Term(TMT6plex); T7(Phospho); K10(TMT6plex) 1 Q9BYG3 Q9BYG3 0 2 0.1354 0 1 1 792.91794 1584.82861 1584.82863 -0.01 -1e-05 2110272.0 CID (Collision Induced Dissociation) MS2 11.65837 30 174.9 60.0 67.1117 20344 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 172.0 178.2 136.2 184.4 165.3 168.4 200.6 192.4 179.8 172.0 Excluded by Method 3.84 1 0.0003003 8.539e-06 False High Sequest HT (A2) Unambiguous DTSATSQSVNGSPQAEQPSLESTSK [K].dTSATSQSVNGsPQAEQPSLESTSk.[E] N-Term(TMT6plex); S12(Phospho); K25(TMT6plex) 3 Q86WB0 Q86WB0-2; Q86WB0-3; Q86WB0 0 3 0.1736 0 1 1 1025.49057 3074.45715 3074.45672 0.14 0.00015 1002909.8125 CID (Collision Induced Dissociation) MS2 2.22679 70 27.7 60.0 67.0001 20304 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 29.9 22.5 23.8 30.8 25.2 29.7 30.6 25.2 27.1 32.0 6.22 1 0.0003003 4.076e-06 False High Sequest HT (A2) Unambiguous EVSEEENTEPEVQEEK [K].eVsEEENtEPEVQEEk.[E] N-Term(TMT6plex); S3(Phospho); T8(Phospho); K16(TMT6plex) 1 P38249 P38249 0 3 0.5039 0 1 1 841.69956 2523.08411 2523.08288 0.49 0.00041 821316.3125 CID (Collision Induced Dissociation) MS2 39.23773 60 34.9 60.0 66.8482 20247 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 9.9 31.2 47.3 13.3 30.7 67.6 14.1 33.1 69.7 32.6 Excluded by Method 5.1 1 0.0003003 1.598e-05 False High Sequest HT (A2) Unambiguous TVPQISDQETEK [K].tVPQIsDQETEk.[R] N-Term(TMT6plex); S6(Phospho); K12(TMT6plex) 23 P0C2I9 P0C2I9; P47100; Q12141; P47098; P0C2J0; P0C2I3; Q12269; Q03855; P0C2I6; P0C2I2; Q99231; Q12414; Q07793; Q04711; Q04670; Q12273; Q03612; Q92393; P0C2I5; Q03434; O13527; P0C2J1; P0C2I7 0 3 0.2267 0 1 1 638.3277 1912.96854 1912.96691 0.85 0.00054 2129652.25 CID (Collision Induced Dissociation) MS2 15.84436 60 96.3 60.0 72.0588 22203 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 28.1 69.4 147.7 37.1 81.9 174.0 41.7 86.6 199.0 97.6 Excluded by Method 5.47 1 0.0003003 4.888e-05 False High Sequest HT (A2) Unambiguous LDEDEDEDDADLSK [K].lDEDEDEDDADLsk.[Y] N-Term(TMT6plex); S13(Phospho); K14(TMT6plex) 2 O95218 O95218-2; O95218 0 2 0.6145 0 1 1 1073.96951 2146.93174 2146.93171 0.02 2e-05 837396.25 CID (Collision Induced Dissociation) MS2 0.0 50 99.4 60.0 76.3282 23813 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 102.2 86.3 72.9 115.0 96.1 94.0 118.9 102.7 106.2 99.3 Excluded by Method 5.37 1 0.0003003 9.043e-07 False High Sequest HT (A2) Unambiguous NAVSTKPTPPPAPEASAESGLSSK [K].nAVSTkPTPPPAPEASAESGLSsk.[V] N-Term(TMT6plex); K6(TMT6plex); S23(Phospho); K24(TMT6plex) 1 P16862 P16862 0 4 0.0843 0 1 1 773.41358 3090.63247 3090.63272 -0.08 -6e-05 2095931.375 CID (Collision Induced Dissociation) MS2 1.4924 30 179.5 47.454 76.2928 23799 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 46.1 135.2 269.4 71.0 136.6 329.9 61.7 170.7 404.4 169.7 Excluded by Method 5.93 1 0.0003003 8.037e-06 False High Sequest HT (A2) Unambiguous DSNELSDSAGEEDSADLK [K].dSNELsDsAGEEDSADLk.[R] N-Term(TMT6plex); S6(Phospho); S8(Phospho); K18(TMT6plex) 2 Q9Y6X9 Q9Y6X9-2; Q9Y6X9 0 3 0.0795 0 1 1 834.01896 2500.04234 2500.04174 0.24 0.0002 430920.46875 CID (Collision Induced Dissociation) MS2 41.04454 30 31.2 60.0 76.1087 23729 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 30.4 32.0 26.7 33.0 24.9 34.9 33.4 31.8 33.0 31.6 Excluded by Method 3.52 1 0.0003003 0.0002204 False High Sequest HT (A2) Unambiguous ATLEEAEGESGVEDDAATGSSNK [K].aTLEEAEGEsGVEDDAATGSSNk.[L] N-Term(TMT6plex); S10(Phospho); K23(TMT6plex) 1 P21304 P21304 0 4 0.2789 0 1 1 702.0734 2805.27177 2805.27154 0.08 6e-05 2026487.25 CID (Collision Induced Dissociation) MS2 0.9306357 70 101.7 22.949 76.0008 23686 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 26.3 94.9 147.6 32.2 86.9 190.6 36.8 92.7 204.2 105.0 6.49 1 0.0003003 1.347e-06 False High Sequest HT (A2) Unambiguous SKPIPIMPASPQK [K].skPIPImPAsPQk.[G] N-Term(TMT6plex); K2(TMT6plex); M7(Oxidation); S10(Phospho); K13(TMT6plex) 9 O00429 O00429-4; O00429-9; O00429; O00429-6; O00429-8; O00429-7; O00429-3; O00429-5; O00429-2 0 3 0.6054 0 1 1 726.41712 2177.2368 2177.23722 -0.2 -0.00014 857107.0 CID (Collision Induced Dissociation) MS2 12.3702 50 213.5 60.0 75.6328 23550 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 221.8 223.9 166.2 236.7 212.0 203.9 247.2 219.9 198.8 204.4 Excluded by Method 4.84 1 0.0003003 2.789e-06 False High Sequest HT (A2) Unambiguous EDSAPVAAGESESSL [K].eDsAPVAAGESESSL.[-] N-Term(TMT6plex); S3(Phospho) 1 P25567 P25567 0 2 0.5358 0 1 1 879.38755 1757.76783 1757.76799 -0.09 -8e-05 5150012.0 CID (Collision Induced Dissociation) MS2 11.25033 90 184.5 60.0 76.0025 23687 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 37.6 151.9 298.5 51.2 144.1 359.1 58.6 169.6 383.0 191.2 4.89 1 0.0003003 8.188e-07 False High Sequest HT (A2) Unambiguous SSSPELVTHLK [K].sSsPELVTHLk.[W] N-Term(TMT6plex); S3(Phospho); K11(TMT6plex) 1 Q07960 Q07960 0 3 0.0204 0 1 1 579.31819 1735.94003 1735.93957 0.26 0.00015 6784718.5 CID (Collision Induced Dissociation) MS2 6.666345 90 122.0 22.107 77.198 24143 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 136.4 114.3 90.8 124.0 123.8 120.1 143.4 119.1 122.6 125.3 4.91 1 0.0003003 0.0002578 False High Sequest HT (A2) Unambiguous KDESDSENDIEIK [K].kDEsDsENDIEIk.[G] N-Term(TMT6plex); K1(TMT6plex); S4(Phospho); S6(Phospho); K13(TMT6plex) 1 P32499 P32499 1 3 0.5281 0 1 1 790.37571 2369.11258 2369.11295 -0.15 -0.00012 1602891.125 CID (Collision Induced Dissociation) MS2 23.86579 40 174.7 60.0 76.8118 23999 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 68.3 142.6 226.3 69.7 144.9 283.8 91.9 177.3 358.7 183.2 Excluded by Method 5.51 1 0.0003003 2.39e-05 False High Sequest HT (A2) Unambiguous EENLQQEDDGEVESSGALEK [K].eENLQQEDDGEVEsSGALEk.[K] N-Term(TMT6plex); S14(Phospho); K20(TMT6plex) 1 P46949 P46949 0 3 0.0227 0 1 1 915.41985 2744.24501 2744.25517 -3.7 -0.00338 1778057.625 CID (Collision Induced Dissociation) MS2 45.07636 10 38.4 60.0 76.7385 23971 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 38.3 29.8 39.6 41.3 37.0 49.5 35.1 35.4 47.4 30.2 Excluded by Method 3.97 1 0.0003003 0.0002432 False High Sequest HT (A2) Unambiguous YNDVIEDESDDDNMSTDEAK [K].yNDVIEDEsDDDNmSTDEAk.[N] N-Term(TMT6plex); S9(Phospho); M14(Oxidation); K20(TMT6plex) 1 P48415 P48415 0 3 0.213 0 1 1 953.73346 2859.18584 2859.18035 1.92 0.00183 313923.8125 CID (Collision Induced Dissociation) MS2 0.0 40 40.5 60.0 76.6637 23942 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 12.0 32.2 53.5 12.8 35.5 79.9 18.0 39.6 78.7 42.8 Excluded by Method 4.6 1 0.0003003 9.443e-05 False High Sequest HT (A2) Unambiguous FTPPAESGSPSK [K].fTPPAESGsPSk.[E] N-Term(TMT6plex); S9(Phospho); K12(TMT6plex) 1 Q70E73 Q70E73 0 3 0.2134 0 1 1 581.63051 1742.87697 1742.87664 0.19 0.00011 984045.9375 CID (Collision Induced Dissociation) MS2 12.50274 50 105.0 60.0 72.9048 22525 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 108.9 108.9 89.6 108.6 91.6 103.2 124.0 107.4 99.7 107.9 Excluded by Method 5.67 1 0.0003003 2.394e-05 False High Sequest HT (A2) Unambiguous RLLGDSDSEEEQK [K].rLLGDsDsEEEQk.[E] N-Term(TMT6plex); S6(Phospho); S8(Phospho); K13(TMT6plex) 1 Q9BW71 Q9BW71 1 3 0.5314 0 1 1 708.66165 2123.97039 2123.96633 1.91 0.00135 1002470.4375 CID (Collision Induced Dissociation) MS2 0.0 50 103.2 60.0 72.9025 22524 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 91.6 101.7 83.1 116.5 95.8 99.1 111.9 117.3 106.5 108.0 Excluded by Method 5.25 1 0.0003003 0.0001708 False High Sequest HT (A2) Unambiguous DSISAVSSEKVSPSK [K].dSISAVSSEkVsPSk.[S] N-Term(TMT6plex); K10(TMT6plex); S12(Phospho); K15(TMT6plex) 1 P46821 P46821 1 3 0.1588 0 1 1 763.41695 2288.23628 2288.23537 0.4 0.0003 1904364.5 CID (Collision Induced Dissociation) MS2 0.0 10 97.4 60.0 72.5073 22373 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 100.0 95.9 72.7 122.6 96.9 83.7 113.4 104.3 91.2 93.7 Excluded by Method 2.96 1 0.0003003 0.0001592 False High Sequest HT (A2) Unambiguous GTDTQTPAVLSPSK [K].gTDTQTPAVLsPSk.[T] N-Term(TMT6plex); S11(Phospho); K14(TMT6plex) 3 P46087 P46087-2; P46087; P46087-4 0 3 0.1071 0 1 1 647.343 1940.01446 1940.01419 0.14 9e-05 15367385.0 CID (Collision Induced Dissociation) MS2 8.427844 80 182.0 10.417 72.4833 22364 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 182.3 179.6 152.2 186.5 162.2 177.5 216.9 206.2 185.9 170.7 4.76 1 0.0003003 3.547e-05 False High Sequest HT (A2) Unambiguous RTCPSLSPTSPLNNK [K].rTcPSLsPTSPLNNk.[G] N-Term(TMT6plex); C3(Carbamidomethyl); S7(Phospho); K15(TMT6plex) 2 O75925 O75925; O75925-2 1 3 0.1367 0 1 1 737.38463 2210.13934 2210.14047 -0.51 -0.00038 732231.5 CID (Collision Induced Dissociation) MS2 20.58016 50 50.6 60.0 72.4128 22338 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 44.6 43.2 56.5 44.9 44.6 61.5 45.8 49.0 70.4 45.6 Excluded by Method 4.39 1 0.0003003 0.0003687 False High Sequest HT (A2) Unambiguous SQVDGAGDVSNEEIAADDTK [K].sQVDGAGDVsNEEIAADDTk.[K] N-Term(TMT6plex); S10(Phospho); K20(TMT6plex) 1 P53438 P53438 0 3 0.2098 0 1 1 853.73366 2559.18642 2559.18636 0.02 2e-05 508225.28125 CID (Collision Induced Dissociation) MS2 0.0 50 22.0 60.0 73.086 22593 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 8.2 19.9 32.2 8.1 17.6 36.7 11.0 20.4 44.6 21.0 Excluded by Method 2.86 1 0.0003003 0.0002409 False High Sequest HT (A2) Unambiguous DETFGEYSDNEEK [K].dETFGEYsDNEEk.[A] N-Term(TMT6plex); S8(Phospho); K13(TMT6plex) 2 P32004-2 P32004-3; P32004-2 0 3 0.1662 0 1 1 700.97306 2100.90463 2100.9051 -0.22 -0.00016 708393.9375 CID (Collision Induced Dissociation) MS2 38.74318 70 218.7 60.0 73.2256 22643 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 219.6 213.5 155.9 272.4 206.2 212.5 259.2 216.1 227.8 203.9 3.85 1 0.0003003 3.782e-06 False High Sequest HT (A2) Unambiguous EAPAEGEAAEPGSPTAAEGEAASAASSTSSPK [K].eAPAEGEAAEPGSPTAAEGEAASAASSTSsPk.[A] N-Term(TMT6plex); S30(Phospho); K32(TMT6plex) 1 P29966 P29966 0 4 0.037 0 1 1 864.15357 3453.59243 3453.59467 -0.65 -0.00056 1384528.125 CID (Collision Induced Dissociation) MS2 0.0 60 140.0 47.116 74.641 23171 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 148.5 148.1 110.7 162.9 134.2 123.6 155.1 158.7 134.2 124.4 Excluded by Method 4.86 1 0.0003003 1.695e-06 False High Sequest HT (A2) Unambiguous GSEGYLAATYPTVGQTSPRARK [K].gSEGYLAATYPTVGQTsPRARk.[S] N-Term(TMT6plex); S17(Phospho); K22(TMT6plex) 3 P21359 P21359-2; P21359-6; P21359 2 4 0.0525 0 1 1 712.87539 2848.47972 2848.47588 1.35 0.00096 543869.5625 CID (Collision Induced Dissociation) MS2 0.0 40 43.9 60.0 74.3934 23076 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 40.1 39.5 38.0 43.1 37.8 45.0 58.1 49.9 42.2 45.4 Excluded by Method 4.57 1 0.0003003 0.0001464 False High Sequest HT (A2) Unambiguous VFDDESDEK [K].vFDDEsDEk.[E] N-Term(TMT6plex); S6(Phospho); K9(TMT6plex) 1 O43719 O43719 0 2 0.3061 0 1 1 811.37251 1621.73774 1621.73987 -1.31 -0.00106 2003377.125 CID (Collision Induced Dissociation) MS2 13.68588 50 227.6 60.0 74.3487 23058 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 245.4 225.4 186.3 272.2 195.6 204.5 271.9 243.7 232.9 198.0 Excluded by Method 4.41 1 0.0003003 0.000155 False High Sequest HT (A2) Unambiguous AAAYDISEDEED [K].aAAYDIsEDEED.[-] N-Term(TMT6plex); S7(Phospho) 1 P49585 P49585 0 2 0.2408 0 1 1 818.82745 1636.64763 1636.64647 0.71 0.00058 5737237.5 CID (Collision Induced Dissociation) MS2 0.0 60 276.2 29.811 73.6099 22785 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 292.8 272.4 244.2 257.8 268.3 301.6 303.6 268.8 283.5 268.7 Excluded by Method 4.36 1 0.0003003 4.309e-06 False High Sequest HT (A2) Unambiguous YAAAAPAATSAASGDAAPAEEAAAEEEEESDDDMGFGLFD [K].yAAAAPAATSAASGDAAPAEEAAAEEEEEsDDDmGFGLFD.[-] N-Term(TMT6plex); S30(Phospho); M34(Oxidation) 1 P05317 P05317 0 4 0.4764 0 1 1 1062.18998 4245.7381 4245.73286 1.23 0.00131 5575279.0 CID (Collision Induced Dissociation) MS2 4.807242 80 27.9 9.083 129.0895 43669 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 2.9 23.8 41.2 7.0 24.5 54.1 11.2 23.5 59.5 31.4 4.87 1 0.0003003 0.0005289 False High Sequest HT (A2) Unambiguous NNSNDIVNAIMELTM [K].nNSNDIVNAImELTm.[-] N-Term(TMT6plex); M11(Oxidation); M15(Oxidation) 3 Q13765 Q13765; E9PAV3; E9PAV3-2 0 3 0.4644 0 1 1 647.31511 1939.93078 1939.93024 0.28 0.00018 3780163.5 CID (Collision Induced Dissociation) MS2 3.977842 70 169.7 44.792 129.6784 43927 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 143.1 177.7 159.0 160.3 154.3 181.9 192.3 173.7 193.3 161.2 5.19 1 0.0003003 4.906e-05 False High Sequest HT (A2) Unambiguous SLHPWYGITPTSSPK [K].sLHPWYGItPTSsPk.[T] N-Term(TMT6plex); T9(Phospho); S13(Phospho); K15(TMT6plex) 1 Q8N3F8 Q8N3F8 0 3 0.0098 0 1 1 763.70955 2289.11411 2289.11221 0.83 0.00063 1181777.375 CID (Collision Induced Dissociation) MS2 2.578469 50 85.7 60.0 106.9351 35277 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 78.2 85.0 75.8 87.3 82.6 85.4 83.9 93.3 101.0 84.3 Excluded by Method 5.1 1 0.0003003 7.284e-05 False High Sequest HT (A2) Unambiguous IGGDAATTVNNSTPDFGFGGQK [K].iGGDAATTVNNStPDFGFGGQk.[R] N-Term(TMT6plex); T13(Phospho); K22(TMT6plex) 1 Q92945 Q92945 0 3 0.1236 0 1 1 898.10579 2692.30283 2692.302 0.31 0.00028 521072.75 CID (Collision Induced Dissociation) MS2 6.264137 50 37.0 60.0 106.6446 35167 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 35.5 42.9 37.0 29.9 37.5 36.3 37.9 39.0 35.4 38.4 Excluded by Method 4.61 1 0.0003003 6.978e-06 False High Sequest HT (A2) Unambiguous ISLSPSTESLADSK [K].iSLSPsTEsLADSk.[S] N-Term(TMT6plex); S6(Phospho); S9(Phospho); K14(TMT6plex) 1 P31374 P31374 0 2 0.3308 0 1 1 1027.00095 2052.99461 2052.99076 1.88 0.00193 314672.78125 CID (Collision Induced Dissociation) MS2 0.0 30 106.9 60.0 106.6404 35165 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 27.1 78.6 159.8 38.4 93.4 200.2 37.6 98.7 224.3 111.0 Excluded by Method 3.99 1 0.0003003 0.0003175 False High Sequest HT (A2) Unambiguous GLIVYCVTSPK [K].gLIVYcVTsPk.[K] N-Term(TMT6plex); C6(Carbamidomethyl); S9(Phospho); K11(TMT6plex) 2 Q8TEU7 Q8TEU7; Q8TEU7-4 0 3 0.1872 0 1 1 592.32382 1774.9569 1774.95787 -0.55 -0.00032 1071695.5 CID (Collision Induced Dissociation) MS2 0.0 70 105.7 60.0 106.627 35160 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 105.0 105.8 83.9 120.9 109.5 106.8 115.3 100.0 104.4 105.3 3.9 1 0.0003003 8.298e-05 False High Sequest HT (A2) Unambiguous EDVTPPLSPDDLK [K].eDVtPPLsPDDLk.[Y] N-Term(TMT6plex); T4(Phospho); S8(Phospho); K13(TMT6plex) 4 Q9Y4B5 Q9Y4B5-3; Q9Y4B5-2; Q9Y4B5-4; Q9Y4B5 0 3 0.335 0 1 1 681.99449 2043.96892 2043.96929 -0.18 -0.00012 664128.75 CID (Collision Induced Dissociation) MS2 11.52615 20 53.4 60.0 106.5361 35125 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 42.2 47.2 56.9 49.1 46.2 73.2 37.9 54.5 77.8 49.3 Excluded by Method 3.91 1 0.0003003 0.0003513 False High Sequest HT (A2) Unambiguous TGPFEVGVHVLPK [K].tGPFEVGVHVLPk.[I] N-Term(TMT6plex); T1(Phospho); K13(TMT6plex) 1 P39954 P39954 0 3 0.5804 0 1 1 640.02527 1918.06125 1918.06036 0.47 0.0003 956472.75 CID (Collision Induced Dissociation) MS2 19.43534 40 74.6 60.0 108.6729 35923 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 26.5 54.2 116.4 26.8 70.0 139.3 33.2 70.1 132.0 77.8 Excluded by Method 4.29 1 0.0003003 0.0004077 False High Sequest HT (A2) Unambiguous DVQSPGFLNEPLSSK [K].dVQsPGFLNEPLSSk.[S] N-Term(TMT6plex); S4(Phospho); K15(TMT6plex) 4 Q8NG31 Q8NG31-4; Q8NG31-3; Q8NG31-2; Q8NG31 0 3 0.3645 0 1 1 719.37321 2156.10507 2156.10407 0.46 0.00033 1092067.0 CID (Collision Induced Dissociation) MS2 8.750067 60 37.2 60.0 107.4087 35451 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 36.5 43.1 32.7 36.5 36.0 40.2 33.9 34.0 42.3 36.5 Excluded by Method 4.17 1 0.0003003 0.0001137 False High Sequest HT (A2) Unambiguous AYSPTCSPTLGFK [K].aYsPTcsPTLGFk.[E] N-Term(TMT6plex); S3(Phospho); C6(Carbamidomethyl); S7(Phospho); K13(TMT6plex) 1 Q5TGY3 Q5TGY3 0 3 0.0468 0 1 1 682.98503 2046.94053 2046.9413 -0.38 -0.00026 480214.28125 CID (Collision Induced Dissociation) MS2 0.0 50 143.3 60.0 108.0655 35697 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 141.1 143.8 129.0 126.6 129.2 139.9 163.2 162.6 160.4 137.3 Excluded by Method 3.63 1 0.0003003 0.0004018 False High Sequest HT (A2) Unambiguous SHYADVDPENQNFLLESNLGK [K].sHyADVDPENQNFLLESNLGk.[K] N-Term(TMT6plex); Y3(Phospho); K21(TMT6plex) 1 Q96QD8 Q96QD8 0 3 0.0031 0 1 1 976.8111 2928.41873 2928.41809 0.22 0.00021 498339.90625 CID (Collision Induced Dissociation) MS2 26.15559 80 15.9 60.0 108.0039 35674 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 15.1 12.9 12.6 12.1 16.3 17.8 20.7 15.2 18.1 17.7 3.19 1 0.0003003 0.000265 False High Sequest HT (A2) Unambiguous IGPLGLSPK [K].iGPLGLsPk.[K] N-Term(TMT6plex); S7(Phospho); K9(TMT6plex) 2 P0CX53 P30050; P0CX53 0 3 0.5485 0 1 1 473.95111 1419.83877 1419.83767 0.77 0.00036 407016.21875 CID (Collision Induced Dissociation) MS2 0.0 50 189.0 60.0 108.3185 35791 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 175.4 184.7 157.4 215.3 166.9 194.7 199.4 184.8 219.9 191.9 Excluded by Method 4.43 1 0.0003003 0.0001203 False High Sequest HT (A2) Unambiguous GSLEEIIAEGQK [K].gSLEEIIAEGQk.[K] N-Term(TMT6plex); K12(TMT6plex) 1 P02400 P02400 0 3 0.5 0 1 1 578.00103 1731.98853 1731.98929 -0.44 -0.00025 901055.0 CID (Collision Induced Dissociation) MS2 7.902421 90 26.3 60.0 104.8414 34488 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 14.4 18.4 34.8 14.2 24.7 45.1 16.8 25.0 41.5 28.5 2.54 1 0.0003003 0.000536 False High Sequest HT (A2) Unambiguous RQSSTPSAPELGQQPDVNISEWK [K].rQSsTPSAPELGQQPDVNISEWk.[D] N-Term(TMT6plex); S4(Phospho); K23(TMT6plex) 4 Q93100 Q93100-2; Q93100; Q93100-3; Q93100-4 1 4 0.0116 0 1 1 773.89215 3092.54678 3092.54541 0.44 0.00034 673169.8125 CID (Collision Induced Dissociation) MS2 46.45343 40 61.1 60.0 104.5583 34382 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 66.4 61.0 48.1 58.4 57.0 63.2 66.0 65.9 60.9 63.7 Excluded by Method 5.18 1 0.0003003 1.051e-05 False High Sequest HT (A2) Unambiguous AFGPGLQGGSAGSPARFTIDTK [K].aFGPGLQGGSAGsPARFTIDTk.[G] N-Term(TMT6plex); S13(Phospho); K22(TMT6plex) 2 P21333 P21333; P21333-2 1 3 0.0585 0 1 1 891.79923 2673.38312 2673.38019 1.1 0.00098 1071899.625 CID (Collision Induced Dissociation) MS2 7.465587 20 77.6 60.0 104.5143 34365 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 82.9 80.8 65.6 72.4 76.4 73.4 87.2 81.3 81.6 74.3 Excluded by Method 3.76 1 0.0003003 9.454e-05 False High Sequest HT (A2) Unambiguous VSAEAEVAPVSPEVTQEVVEEHCASPEDK [K].vSAEAEVAPVsPEVTQEVVEEHcAsPEDk.[T] N-Term(TMT6plex); S11(Phospho); C23(Carbamidomethyl); S25(Phospho); K29(TMT6plex) 1 P46821 P46821 0 4 0.1268 0 1 1 935.93239 3740.70771 3740.70556 0.58 0.00054 612763.0 CID (Collision Induced Dissociation) MS2 0.0 50 217.9 60.0 105.061 34571 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 230.0 220.3 173.1 250.9 199.0 194.6 259.8 227.3 220.4 203.5 Excluded by Method 6.31 1 0.0003003 6.968e-06 False High Sequest HT (A2) Unambiguous NNSPILETLPSNEIK [K].nNsPILETLPSNEIk.[T] N-Term(TMT6plex); S3(Phospho); K15(TMT6plex) 1 P38853 P38853 0 3 0.2547 0 1 1 736.39582 2207.17291 2207.17249 0.19 0.00014 5212663.0 CID (Collision Induced Dissociation) MS2 8.45891 50 110.5 19.108 106.0755 34948 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 27.8 87.1 169.5 35.7 92.3 207.6 32.3 108.0 233.6 111.5 Excluded by Method 3.22 1 0.0003003 0.0003034 False High Sequest HT (A2) Unambiguous GLINDPDVNNTFNINK [K].gLINDPDVNNTFNINk.[G] N-Term(TMT6plex); K16(TMT6plex) 1 P32449 P32449 0 3 0.5527 0 1 1 749.41093 2246.21825 2246.21812 0.06 4e-05 2398883.25 CID (Collision Induced Dissociation) MS2 1.486324 40 113.3 58.699 105.7811 34839 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 29.5 99.7 188.1 37.3 93.3 234.4 111.0 219.2 120.8 Excluded by Method 4.74 1 0.0003003 6.007e-06 False High Sequest HT (A2) Unambiguous DEGIGSPDIWEDEK [K].dEGIGsPDIWEDEk.[A] N-Term(TMT6plex); S6(Phospho); K14(TMT6plex) 1 Q01664 Q01664 0 3 0.4593 0 1 1 710.00189 2127.99111 2127.98877 1.1 0.00078 2336911.75 CID (Collision Induced Dissociation) MS2 8.505637 90 146.3 60.0 105.7014 34809 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 158.1 155.8 101.7 151.7 146.5 130.0 177.2 155.9 137.5 148.3 5.16 1 0.0003003 1.139e-05 False High Sequest HT (A2) Unambiguous GRAEGEWEDQEALDYFSDK [K].gRAEGEWEDQEALDYFsDk.[E] N-Term(TMT6plex); S17(Phospho); K19(TMT6plex) 3 Q9NYF8 Q9NYF8-3; Q9NYF8; Q9NYF8-2 1 3 0.191 0 1 1 928.42117 2783.24895 2783.26019 -4.04 -0.00375 549078.25 CID (Collision Induced Dissociation) MS2 85.38993 40 50.3 60.0 105.0747 34576 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 44.5 47.2 44.3 56.0 44.5 47.9 60.7 56.4 51.2 50.2 Excluded by Method 4.66 1 0.0003003 0.0004414 False High Sequest HT (A2) Unambiguous TISQEFLTPGK [K].tIsQEFLTPGk.[L] N-Term(TMT6plex); S3(Phospho); K11(TMT6plex) 4 Q0ZGT2 Q0ZGT2-2; Q0ZGT2-4; Q0ZGT2; Q0ZGT2-3 0 2 0.2302 0 1 1 879.97533 1758.94339 1758.94432 -0.53 -0.00047 788904.5625 CID (Collision Induced Dissociation) MS2 0.0 60 73.4 60.0 105.3625 34681 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 76.4 78.7 58.9 72.2 73.3 78.7 77.8 80.3 70.3 67.4 Excluded by Method 4.3 1 0.0003003 0.0001086 False High Sequest HT (A2) Unambiguous VQGEAVSNIQENTQTPTVQEESEEEEVDETGVEVK [K].vQGEAVSNIQENTQTPTVQEEsEEEEVDETGVEVk.[D] N-Term(TMT6plex); S22(Phospho); K35(TMT6plex) 3 Q13765 Q13765; E9PAV3; E9PAV3-2 0 4 0.0329 0 1 1 1100.52474 4399.07712 4399.06016 3.86 0.00424 238891.78125 CID (Collision Induced Dissociation) MS2 0.0 40 32.7 60.0 105.2994 34658 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 34.1 29.1 24.6 33.5 35.0 37.3 37.8 37.5 29.8 28.7 Excluded by Method 5.78 1 0.0003003 3.963e-06 False High Sequest HT (A2) Unambiguous MSPITFSDVDNK [K].msPITFSDVDNk.[I] N-Term(TMT6plex); S2(Phospho); K12(TMT6plex) 1 P53148 P53148 0 3 0.1034 0 1 1 631.31416 1891.92794 1891.92769 0.13 8e-05 423193.21875 CID (Collision Induced Dissociation) MS2 65.85012 50 26.8 60.0 108.8374 35982 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 12.6 21.2 36.3 9.9 21.9 45.4 13.1 31.6 47.9 28.1 Excluded by Method 2.9 1 0.0003003 0.0002686 False High Sequest HT (A2) Unambiguous FLNINRVCSSDSDLPGSDIK [K].fLNINRVcsSDSDLPGSDIk.[S] N-Term(TMT6plex); C8(Carbamidomethyl); S9(Phospho); K20(TMT6plex) 1 P27466 P27466 1 3 0.1163 0 1 1 925.79745 2775.37781 2775.37887 -0.38 -0.00035 1127483.5 CID (Collision Induced Dissociation) MS2 48.52167 40 68.9 55.488 112.9112 37504 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 19.9 52.9 105.8 20.9 57.3 128.4 24.0 64.1 146.9 68.8 Excluded by Method 4.3 1 0.0003003 7.245e-05 False High Sequest HT (A2) Unambiguous VVVAENFDEIVNNENK [K].vVVAENFDEIVNNENk.[D] N-Term(TMT6plex); K16(TMT6plex) 1 P30101 P30101 0 3 0.4164 0 1 1 764.41487 2291.23007 2291.22835 0.75 0.00057 3731949.0 CID (Collision Induced Dissociation) MS2 1.575351 60 100.8 60.0 113.1118 37578 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 99.6 110.9 83.5 96.6 103.2 96.8 108.6 104.4 97.9 106.9 Excluded by Method 5.5 1 0.0003003 1.039e-07 False High Sequest HT (A2) Unambiguous LTFDTTFSPNTGK [K].lTFDTTFsPNTGk.[K] N-Term(TMT6plex); S8(Phospho); K13(TMT6plex) 3 P45880 P45880; P45880-2; P45880-1 0 3 0.1964 0 1 1 656.33713 1966.99683 1966.99273 2.09 0.00137 3021903.0 CID (Collision Induced Dissociation) MS2 3.208061 80 150.1 53.276 112.7518 37445 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 169.9 156.9 127.0 151.4 135.6 141.9 186.5 165.3 142.6 123.8 4.94 1 0.0003003 8.107e-05 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVSsDEEF.[-] N-Term(TMT6plex); S9(Phospho) 1 P16521 P16521 0 2 0.0455 0 1 1 885.37138 1769.73548 1769.73562 -0.08 -7e-05 23609960.0 CID (Collision Induced Dissociation) MS2 0.0 70 244.5 18.925 112.705 37428 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 60.0 201.2 403.7 67.8 190.1 483.3 76.0 210.2 498.0 254.8 4.62 1 0.0003003 3.444e-06 False High Sequest HT (A2) Unambiguous KEESEESDDDMGFGLFD [K].kEESEEsDDDmGFGLFD.[-] N-Term(TMT6plex); K1(TMT6plex); S7(Phospho); M11(Oxidation) 3 P05387 P05386; P05386-2; P05387 1 2 0.0952 0 1 1 1252.52825 2504.04922 2504.04642 1.12 0.0014 1075267.75 CID (Collision Induced Dissociation) MS2 0.0 20 119.5 60.0 112.1563 37222 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 131.5 132.5 90.1 128.9 108.3 111.7 125.2 128.1 123.0 115.3 Excluded by Method 4.2 1 0.0003003 0.0001677 False High Sequest HT (A2) Unambiguous NYWDVNDSPIIK [K].nYWDVNDsPIIk.[V] N-Term(TMT6plex); S8(Phospho); K12(TMT6plex) 1 P23201 P23201 0 2 0.5074 0 1 1 1001.5099 2002.01253 2002.00872 1.9 0.00191 472249.28125 CID (Collision Induced Dissociation) MS2 37.97894 40 68.0 60.0 112.7409 37441 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 15.1 55.8 108.8 23.4 55.5 125.0 27.3 69.9 128.9 70.3 Excluded by Method 4.08 1 0.0003003 0.0001915 False High Sequest HT (A2) Unambiguous DNLSDTEEVIVIK [K].dNLsDTEEVIVIk.[K] N-Term(TMT6plex); S4(Phospho); K13(TMT6plex) 1 Q08951 Q08951 0 3 0.0082 0 1 1 671.69013 2013.05583 2013.05572 0.05 4e-05 3239709.25 CID (Collision Induced Dissociation) MS2 9.25889 60 163.6 42.179 113.1265 37584 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 40.0 139.9 270.3 45.8 122.7 299.2 65.8 149.4 337.6 165.7 Excluded by Method 3.68 1 0.0003003 0.0002399 False High Sequest HT (A2) Unambiguous VFVGGLSPDTSEEQIK [K].vFVGGLsPDTSEEQIk.[E] N-Term(TMT6plex); S7(Phospho); K16(TMT6plex) 3 O14979 O14979-3; O14979; O14979-2 0 2 0.1367 0 1 1 1122.58195 2244.15662 2244.1565 0.05 6e-05 331753.53125 CID (Collision Induced Dissociation) MS2 0.0 20 24.2 60.0 113.3803 37678 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 26.1 26.2 18.8 19.7 24.9 22.5 25.0 28.4 25.8 24.7 Excluded by Method 4.9 1 0.0003003 0.0002605 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVSsDEEF.[-] N-Term(TMT6plex); S9(Phospho) 1 P16521 P16521 0 2 0.0223 0 1 1 885.37089 1769.73451 1769.73562 -0.63 -0.00056 4467109.5 CID (Collision Induced Dissociation) MS2 0.0 70 191.2 45.598 113.8413 37847 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 48.0 146.1 315.9 55.4 153.7 366.5 62.1 172.9 397.0 194.4 4.48 1 0.0003003 2.302e-06 False High Sequest HT (A2) Unambiguous VTFSVSPIEATEK [K].vTFSVsPIEATEk.[C] N-Term(TMT6plex); S6(Phospho); K13(TMT6plex) 3 Q9ULD2 Q9ULD2-5; Q9ULD2; Q9ULD2-2 0 3 0.0538 0 1 1 649.34892 1946.03221 1946.02878 1.76 0.00114 370748.28125 CID (Collision Induced Dissociation) MS2 11.66901 50 71.4 60.0 113.378 37677 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 77.0 67.6 69.8 70.5 58.2 83.1 65.1 73.6 83.7 65.8 Excluded by Method 3.72 1 0.0003003 0.0003499 False High Sequest HT (A2) Unambiguous VQGEAVSNIQENTQTPTVQEESEEEEVDETGVEVK [K].vQGEAVSNIQENTQTPTVQEEsEEEEVDETGVEVk.[D] N-Term(TMT6plex); S22(Phospho); K35(TMT6plex) 3 Q13765 Q13765; E9PAV3; E9PAV3-2 0 4 0.0379 0 1 1 1100.51595 4399.04197 4399.06016 -4.14 -0.00455 650974.75 CID (Collision Induced Dissociation) MS2 20.37229 50 24.6 60.0 113.4361 37698 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 26.1 26.4 17.5 22.2 24.7 23.1 30.4 24.0 28.9 22.9 Excluded by Method 5.28 1 0.0003003 8.824e-05 False High Sequest HT (A2) Unambiguous AAADALSDLEIK [K].aAADALsDLEIk.[D] N-Term(TMT6plex); S7(Phospho); K12(TMT6plex) 1 P09938 P09938 0 3 0.1698 0 1 1 585.64933 1754.93343 1754.93415 -0.41 -0.00024 696550.0625 CID (Collision Induced Dissociation) MS2 0.0 50 202.9 60.0 113.5519 37741 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 54.1 181.0 306.7 61.4 151.6 373.4 77.3 186.1 430.6 206.7 Excluded by Method 3.18 1 0.0003003 2.438e-05 False High Sequest HT (A2) Unambiguous TLSVAAAFNEDEDSEPEEMPPEAK [K].tLSVAAAFNEDEDsEPEEmPPEAk.[M] N-Term(TMT6plex); S14(Phospho); M19(Oxidation); K24(TMT6plex) 1 Q8WW12 Q8WW12 0 4 0.3182 0 1 1 790.8635 3160.43215 3160.43214 0.0 0.0 1180389.125 CID (Collision Induced Dissociation) MS2 16.15122 50 53.0 55.241 109.1846 36113 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 40.8 45.9 49.9 43.6 46.2 76.9 43.1 57.3 72.8 53.0 Excluded by Method 5.72 1 0.0003003 1.876e-06 False High Sequest HT (A2) Unambiguous IENQQGVNNFDEILK [K].iENQQGVNNFDEILk.[V] N-Term(TMT6plex); K15(TMT6plex) 1 P00549 P00549 0 3 0.4957 0 1 1 740.40784 2219.20896 2219.20722 0.79 0.00058 2267291.5 CID (Collision Induced Dissociation) MS2 8.82146 60 72.6 57.6 109.017 36050 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 19.6 64.4 111.9 20.7 63.9 140.7 26.1 68.9 146.2 63.5 Excluded by Method 4.62 1 0.0003003 2.801e-08 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVsSDEEF.[-] N-Term(TMT6plex); S8(Phospho) 1 P16521 P16521 0 2 0.0239 0 1 1 885.37291 1769.73854 1769.73562 1.65 0.00146 1115124.375 CID (Collision Induced Dissociation) MS2 0.0 60 126.7 60.0 110.4375 36585 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 24.0 96.1 202.2 38.6 111.5 241.3 46.9 118.2 268.4 119.9 Excluded by Method 3.76 1 0.0003003 5.175e-05 False High Sequest HT (A2) Unambiguous EVDPSTGELQSLQMPESEGPSSLDPSQEGPTGLK [K].eVDPSTGELQSLQmPESEGPSsLDPSQEGPTGLk.[E] N-Term(TMT6plex); M14(Oxidation); S22(Phospho); K34(TMT6plex) 2 Q13501 Q13501-2; Q13501 0 4 0.0213 0 1 1 1020.9846 4080.91657 4080.92485 -2.03 -0.00207 687451.125 CID (Collision Induced Dissociation) MS2 0.0 50 45.0 60.0 110.4558 36592 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 41.4 44.9 38.0 44.0 46.9 50.8 45.6 50.4 43.1 44.8 Excluded by Method 7.99 1 0.0003003 2.437e-09 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVSsDEEF.[-] N-Term(TMT6plex); S9(Phospho) 1 P16521 P16521 0 2 0.0219 0 1 1 885.37187 1769.73646 1769.73562 0.47 0.00042 9658387.0 CID (Collision Induced Dissociation) MS2 0.0 70 169.0 18.058 111.5728 37003 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 42.9 136.9 306.0 41.3 154.4 348.1 146.7 346.2 167.0 4.57 1 0.0003003 7.37e-07 False High Sequest HT (A2) Unambiguous DNGFDQLPVLTEDGK [K].dNGFDQLPVLTEDGk.[L] N-Term(TMT6plex); K15(TMT6plex) 1 P32582 P32582 0 3 0.4296 0 1 1 702.70887 2106.11206 2106.11192 0.07 5e-05 1375309.125 CID (Collision Induced Dissociation) MS2 0.0 60 90.0 60.0 111.2038 36872 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 25.0 76.4 130.2 25.5 68.6 164.8 35.1 91.5 190.9 91.9 Excluded by Method 4.05 1 0.0003003 1.058e-05 False High Sequest HT (A2) Unambiguous STELLLGVK [K].sTELLLGVk.[T] N-Term(TMT6plex); S1(Phospho); K9(TMT6plex) 2 Q6R327 Q6R327; Q6R327-3 0 2 0.0831 0 1 1 749.43895 1497.87062 1497.86937 0.84 0.00063 2359497.75 CID (Collision Induced Dissociation) MS2 0.0 60 119.2 60.0 111.108 36835 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 126.4 109.0 97.0 123.5 112.1 118.0 132.1 134.4 120.2 119.0 Excluded by Method 3.97 1 0.0003003 0.0002101 False High Sequest HT (A2) Unambiguous DLFDYSPPLHK [K].dLFDYsPPLHk.[N] N-Term(TMT6plex); S6(Phospho); K11(TMT6plex) 3 Q9NYF8 Q9NYF8-3; Q9NYF8; Q9NYF8-2 0 2 0.0133 0 1 1 935.48158 1869.95588 1869.95522 0.35 0.00033 5808320.5 CID (Collision Induced Dissociation) MS2 0.0 30 194.9 60.0 111.1014 36833 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 206.1 206.5 155.6 207.3 194.5 184.9 216.7 205.3 184.0 188.3 Excluded by Method 4.5 1 0.0003003 0.0001002 False High Sequest HT (A2) Unambiguous SSENVQVDLANFEK [K].ssENVQVDLANFEk.[A] N-Term(TMT6plex); S2(Phospho); K14(TMT6plex) 1 P22147 P22147 0 3 0.0126 0 1 1 706.68842 2118.0507 2118.05204 -0.63 -0.00045 1701913.375 CID (Collision Induced Dissociation) MS2 0.0 40 172.1 60.0 111.0201 36803 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 43.3 136.8 267.0 48.2 144.6 323.7 63.0 159.0 355.3 180.4 Excluded by Method 4.77 1 0.0003003 1.739e-05 False High Sequest HT (A2) Unambiguous AGLSPAEPLLSRAGFSPDSSK [K].aGLSPAEPLLSRAGFsPDSSk.[A] N-Term(TMT6plex); S16(Phospho); K21(TMT6plex) 1 O95402 O95402 1 3 0.1127 0 1 1 875.79462 2625.36929 2625.36895 0.13 0.00011 1386150.625 CID (Collision Induced Dissociation) MS2 0.0 40 61.6 60.0 110.6052 36648 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 67.4 65.8 48.8 59.0 57.7 62.5 72.5 63.3 61.7 56.8 Excluded by Method 4.79 1 0.0003003 9.304e-05 False High Sequest HT (A2) Unambiguous GGITTGDISEEETVDNSINTEEYDK [K].gGITTGDIsEEETVDNSINTEEYDk.[L] N-Term(TMT6plex); S9(Phospho); K25(TMT6plex) 1 Q06704 Q06704 0 3 0.0344 0 1 1 1085.50047 3254.48687 3254.48774 -0.27 -0.00029 763681.9375 CID (Collision Induced Dissociation) MS2 0.0 50 34.5 60.0 104.0955 34209 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 11.7 31.2 47.5 8.7 26.4 72.1 11.9 32.0 71.1 32.3 Excluded by Method 7.85 1 0.0003003 9.957e-09 False High Sequest HT (A2) Unambiguous EFSIPRAHAWPSPYK [K].eFSIPRAHAWPsPYk.[D] N-Term(TMT6plex); S12(Phospho); K15(TMT6plex) 1 Q8NFJ5 Q8NFJ5 1 4 0.2801 0 1 1 581.80485 2324.19759 2324.19931 -0.74 -0.00043 1355268.25 CID (Collision Induced Dissociation) MS2 0.0 20 158.9 60.0 98.0026 31932 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 180.7 152.4 122.3 158.8 156.1 158.2 167.0 178.3 144.8 170.0 Excluded by Method 2.82 1 0.0003003 0.0002915 False High Sequest HT (A2) Unambiguous LLEPAYDGASVEQQ [K].lLEPAYDGASVEQQ.[-] N-Term(TMT6plex) 1 P17423 P17423 0 2 0.6329 0 1 1 874.94843 1748.88958 1748.89041 -0.48 -0.00042 926636.8125 CID (Collision Induced Dissociation) MS2 0.0 70 14.7 60.0 97.9027 31894 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 6.5 11.0 20.4 11.5 11.1 28.9 3.9 18.6 20.9 14.6 2.86 1 0.0003003 0.0005006 False High Sequest HT (A2) Unambiguous GSILLTSDEEEEEK [K].gSILLtsDEEEEEk.[G] N-Term(TMT6plex); T6(Phospho); S7(Phospho); K14(TMT6plex) 1 P46949 P46949 0 3 0.1506 0 1 1 733.00343 2196.99574 2196.99663 -0.4 -0.0003 312683.6875 CID (Collision Induced Dissociation) MS2 25.96255 80 25.6 60.0 97.5982 31778 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 11.8 22.6 31.6 12.9 24.7 40.8 13.1 23.2 50.8 24.9 3.85 1 0.0003003 0.0003103 False High Sequest HT (A2) Unambiguous IGEGTYGVVYK [K].iGEGtYGVVYk.[AG] N-Term(TMT6plex); T5(Phospho); K11(TMT6plex) 5 P24941 P24941; P06493; Q00526; P24941-2; P06493-2 0 2 0.097 0 1 1 862.45724 1723.9072 1723.90721 -0.01 -1e-05 32913330.0 CID (Collision Induced Dissociation) MS2 18.38008 40 93.7 8.78 97.4994 31739 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 87.7 94.9 70.0 95.8 94.1 96.4 90.8 102.9 103.9 100.6 Excluded by Method 4.33 1 0.0003003 2.972e-05 False High Sequest HT (A2) Unambiguous IAASLSDDDLK [K].iAASLsDDDLk.[E] N-Term(TMT6plex); S6(Phospho); K11(TMT6plex) 1 P25389 P25389 0 2 0.1284 0 1 1 843.44299 1685.8787 1685.8763 1.42 0.0012 1684666.125 CID (Collision Induced Dissociation) MS2 30.97173 30 47.1 60.0 97.3887 31699 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 17.5 41.8 69.5 17.3 42.5 86.4 20.8 39.2 87.2 48.6 Excluded by Method 4.05 1 0.0003003 0.0005555 False High Sequest HT (A2) Unambiguous DGVLTLANNVTPAK [K].dGVLTLANNVtPAk.[D] N-Term(TMT6plex); T11(Phospho); K14(TMT6plex) 2 P27816 P27816; P27816-6 0 2 0.4291 0 1 1 976.03827 1951.06926 1951.06656 1.38 0.00135 1290253.25 CID (Collision Induced Dissociation) MS2 0.0 50 61.6 60.0 98.9185 32279 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 60.0 63.2 49.0 62.5 60.2 63.6 70.0 64.3 59.1 64.4 Excluded by Method 5.08 1 0.0003003 6.613e-06 False High Sequest HT (A2) Unambiguous VVDYSQFQESDDADEDYGRDSGPPTK [K].vVDYSQFQEsDDADEDYGRDSGPPTk.[K] N-Term(TMT6plex); S10(Phospho); K26(TMT6plex) 1 Q9H1E3 Q9H1E3 1 4 0.1064 0 1 1 865.38784 3458.52951 3458.53134 -0.53 -0.00046 354241.96875 CID (Collision Induced Dissociation) MS2 0.0 70 17.4 60.0 98.7117 32201 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 15.4 17.9 18.4 16.0 15.6 19.6 20.8 19.8 16.1 14.2 5.64 1 0.0003003 4.512e-05 False High Sequest HT (A2) Unambiguous EEDNIDEDEYEEEGEGEESDFDEYK [K].eEDNIDEDEYEEEGEGEEsDFDEYk.[D] N-Term(TMT6plex); S19(Phospho); K25(TMT6plex) 1 Q06839 Q06839 0 3 0.1989 0 1 1 1194.47647 3581.41486 3581.40525 2.68 0.0032 572157.5625 CID (Collision Induced Dissociation) MS2 0.0 70 12.1 60.0 98.6351 32172 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 9.3 14.4 3.7 15.0 23.7 13.2 25.1 16.9 5.63 1 0.0003003 4.534e-06 False High Sequest HT (A2) Unambiguous SHDDGNIDLESDSFLK [K].sHDDGNIDLESDsFLk.[F] N-Term(TMT6plex); S13(Phospho); K16(TMT6plex) 10 P18583 P18583; P18583-4; P18583-6; P18583-5; P18583-10; P18583-9; P18583-2; P18583-3; P18583-7; P18583-8 0 3 0.1154 0 1 1 777.37054 2330.09706 2330.09536 0.73 0.00057 479582.5 CID (Collision Induced Dissociation) MS2 0.0 50 54.6 60.0 98.5434 32137 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 63.0 54.7 43.2 61.1 45.0 48.4 66.8 58.3 47.0 58.2 Excluded by Method 6.15 1 0.0003003 1.021e-06 False High Sequest HT (A2) Unambiguous DESCYDNAEAAFSDDEEDLNSK [K].dEScYDNAEAAFsDDEEDLNSk.[G] N-Term(TMT6plex); C4(Carbamidomethyl); S13(Phospho); K22(TMT6plex) 4 Q68E01 Q68E01; Q68E01-4; Q68E01-2; Q68E01-3 0 3 0.2299 0 1 1 1021.42167 3062.25046 3062.24982 0.21 0.00021 432622.71875 CID (Collision Induced Dissociation) MS2 58.31414 60 14.4 60.0 98.3725 32072 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 11.6 14.3 11.2 16.7 11.2 13.0 12.7 17.6 19.2 16.6 Excluded by Method 4.35 1 0.0003003 2.456e-05 False High Sequest HT (A2) Unambiguous AAEAGGAEEQYGFLTTPTK [K].aAEAGGAEEQYGFLTtPTk.[Q] N-Term(TMT6plex); T16(Phospho); K19(TMT6plex) 1 P46821 P46821 0 4 0.0982 0 1 1 620.55961 2479.21659 2479.21581 0.32 0.0002 877344.4375 CID (Collision Induced Dissociation) MS2 0.0 80 46.9 60.0 98.3284 32055 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 44.7 45.4 37.4 49.4 43.3 50.8 50.3 50.5 48.8 48.8 5.09 1 0.0003003 2.677e-06 False High Sequest HT (A2) Unambiguous EETPPPVEPEEEEDTEDAGLDDWEAMASDEETEK [K].eETPPPVEPEEEEDTEDAGLDDWEAmAsDEETEk.[V] N-Term(TMT6plex); M26(Oxidation); S28(Phospho); K34(TMT6plex) 1 O60841 O60841 0 4 0.0581 0 1 1 1101.47127 4402.86325 4402.83694 5.97 0.00658 706032.1875 CID (Collision Induced Dissociation) MS2 17.34 50 103.4 60.0 99.0701 32337 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 111.5 105.0 78.3 126.0 91.6 91.8 120.5 108.7 96.9 104.0 Excluded by Method 8.61 1 0.0003003 2.556e-06 False High Sequest HT (A2) Unambiguous EVQSILTQEVDNNNK [K].eVQSILTQEVDNNNk.[T] N-Term(TMT6plex); K15(TMT6plex) 1 P47119 P47119 0 3 0.3419 0 1 1 730.39864 2189.18137 2189.1814 -0.01 -1e-05 1553560.625 CID (Collision Induced Dissociation) MS2 1.779076 70 155.6 54.233 97.0026 31550 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 38.3 122.9 241.5 48.4 132.0 302.4 47.1 147.8 308.9 166.7 6.64 1 0.0003003 5.295e-08 False High Sequest HT (A2) Unambiguous ETTSDNEDLLIK [K].eTTsDNEDLLIk.[E] N-Term(TMT6plex); S4(Phospho); K12(TMT6plex) 1 Q04347 Q04347 0 2 0.0878 0 1 1 958.4866 1915.96593 1915.96658 -0.34 -0.00032 7896603.5 CID (Collision Induced Dissociation) MS2 9.608745 30 190.1 47.494 95.4176 30951 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 46.8 160.7 281.7 54.5 150.4 366.0 71.8 177.6 390.3 201.1 Excluded by Method 5.01 1 0.0003003 0.000165 False High Sequest HT (A2) Unambiguous SFDHLISDTK [K].sFDHLISDTk.[A] N-Term(TMT6plex); S1(Phospho); K10(TMT6plex) 3 P21359 P21359-2; P21359-6; P21359 0 3 0.5216 0 1 1 567.62721 1700.86707 1700.86607 0.58 0.00033 703439.875 CID (Collision Induced Dissociation) MS2 5.088344 50 368.5 60.0 95.2376 30884 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 376.4 339.3 314.4 387.7 358.1 364.2 408.7 388.4 367.8 380.4 Excluded by Method 4.39 1 0.0003003 0.0003449 False High Sequest HT (A2) Unambiguous FEDEGAGFEESSETGDYEEK [K].fEDEGAGFEESsETGDYEEk.[A] N-Term(TMT6plex); S12(Phospho); K20(TMT6plex) 1 P46821 P46821 0 3 0.0026 0 1 1 931.72701 2793.16648 2793.17043 -1.42 -0.00132 696972.9375 CID (Collision Induced Dissociation) MS2 49.17144 60 25.0 60.0 95.0438 30813 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 26.2 26.1 23.3 23.6 19.3 27.4 28.7 27.5 25.4 22.5 Excluded by Method 3.89 1 0.0003003 1.934e-05 False High Sequest HT (A2) Unambiguous GDEESGADTVTSPITFEK [K].gDEESGADTVTsPITFEk.[K] N-Term(TMT6plex); S12(Phospho); K18(TMT6plex) 1 P53191 P53191 0 3 0.0879 0 1 1 807.72064 2421.14738 2421.14745 -0.03 -3e-05 1105498.5 CID (Collision Induced Dissociation) MS2 46.10166 60 27.8 60.0 94.4543 30592 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 11.6 18.7 36.7 11.1 31.0 51.2 15.2 31.9 44.3 26.6 Excluded by Method 3.87 1 0.0003003 0.0001149 False High Sequest HT (A2) Unambiguous LFIIRGSPQQIDHAK [K].lFIIRGsPQQIDHAk.[Q] N-Term(TMT6plex); S7(Phospho); K15(TMT6plex) 1 Q92945 Q92945 1 4 0.5926 0 1 1 566.06978 2261.25727 2261.25716 0.05 3e-05 1474266.625 CID (Collision Induced Dissociation) MS2 0.0 20 86.8 60.0 95.8976 31134 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 86.9 88.4 72.0 88.9 81.8 83.9 93.6 97.1 85.6 89.7 Excluded by Method 3.78 1 0.0003003 1.575e-05 False High Sequest HT (A2) Unambiguous SPSLSPSPPSPLEK [K].sPsLSPSPPSPLEk.[T] N-Term(TMT6plex); S1(Phospho); S3(Phospho); K14(TMT6plex) 1 P46821 P46821 0 3 0.0082 0 1 1 681.00688 2041.0061 2041.00601 0.04 3e-05 693565.25 CID (Collision Induced Dissociation) MS2 33.85336 50 201.6 60.0 96.7814 31465 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 212.6 214.5 166.4 203.1 185.8 184.1 236.5 218.1 197.3 197.9 Excluded by Method 3.65 1 0.0003003 0.000412 False High Sequest HT (A2) Unambiguous IEEVLSPEGSPSK [K].iEEVLsPEGsPSk.[S] N-Term(TMT6plex); S6(Phospho); S10(Phospho); K13(TMT6plex) 2 Q9UEY8 Q9UEY8-2; Q9UEY8 0 3 0.5447 0 1 1 663.99141 1989.95968 1989.95873 0.48 0.00032 2821014.5 CID (Collision Induced Dissociation) MS2 0.0 50 219.4 60.0 102.3276 33546 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 217.9 225.1 168.4 241.2 210.0 207.2 263.4 228.5 220.4 212.1 Excluded by Method 4.81 1 0.0003003 0.0002327 False High Sequest HT (A2) Unambiguous LEVTEIVKPSPK [K].lEVTEIVkPsPk.[R] N-Term(TMT6plex); K8(TMT6plex); S10(Phospho); K12(TMT6plex) 2 Q7Z6E9 Q7Z6E9-2; Q7Z6E9 0 4 0.5588 0 1 1 527.56502 2107.23826 2107.23827 0.0 0.0 1406852.0 CID (Collision Induced Dissociation) MS2 4.509638 40 168.6 60.0 102.1262 33471 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 173.7 171.6 114.3 194.6 170.4 148.5 195.1 184.1 168.8 164.7 Excluded by Method 2.38 1 0.0003003 0.0003419 False High Sequest HT (A2) Unambiguous NPDEDEFLINSDDEM [K].nPDEDEFLINsDDEm.[-] N-Term(TMT6plex); S11(Phospho); M15(Oxidation) 1 Q08412 Q08412 0 2 0.6843 0 1 1 1054.41813 2107.82899 2107.82524 1.78 0.00187 1596634.0 CID (Collision Induced Dissociation) MS2 0.0 80 76.1 60.0 102.4278 33582 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 19.6 60.0 123.7 23.0 59.1 149.2 26.6 67.6 160.9 71.0 4.15 1 0.0003003 6.667e-06 False High Sequest HT (A2) Unambiguous VQGEAVSNIQENTQTPTVQEESEEEEVDETGVEVK [K].vQGEAVSNIQENTQTPTVQEEsEEEEVDETGVEVk.[D] N-Term(TMT6plex); S22(Phospho); K35(TMT6plex) 3 Q13765 Q13765; E9PAV3; E9PAV3-2 0 4 0.0568 0 1 1 1100.52144 4399.06394 4399.06016 0.86 0.00095 772410.1875 CID (Collision Induced Dissociation) MS2 4.117719 50 69.5 38.603 102.1088 33464 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 72.1 69.2 57.7 70.8 63.4 66.8 78.3 85.0 71.9 60.2 Excluded by Method 6.87 1 0.0003003 8.819e-07 False High Sequest HT (A2) Unambiguous DNEELQEIDNVSLDEPK [K].dNEELQEIDNVsLDEPk.[I] N-Term(TMT6plex); S12(Phospho); K17(TMT6plex) 1 P25037 P25037 0 3 0.556 0 1 1 842.40686 2525.20602 2525.20603 0.0 0.0 7702194.0 CID (Collision Induced Dissociation) MS2 0.4328085 60 124.8 16.317 104.0637 34196 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 33.2 95.6 182.2 44.5 99.8 212.3 43.2 120.4 281.8 135.1 Excluded by Method 4.82 1 0.0003003 2.869e-06 False High Sequest HT (A2) Unambiguous VYYFQGGNNELGTAVGK [K].vYYFQGGNNELGTAVGk.[L] N-Term(TMT6plex); K17(TMT6plex) 1 P14120 P14120 0 3 0.4826 0 1 1 759.07608 2275.21368 2275.2123 0.61 0.00046 1371615.125 CID (Collision Induced Dissociation) MS2 7.476518 60 64.7 60.0 103.8919 34136 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 21.3 49.1 89.3 23.6 54.7 122.6 26.4 65.3 126.6 67.7 Excluded by Method 2.88 1 0.0003003 1.14e-05 False High Sequest HT (A2) Unambiguous GRAEGEWEDQEALDYFSDK [K].gRAEGEWEDQEALDYFsDk.[E] N-Term(TMT6plex); S17(Phospho); K19(TMT6plex) 3 Q9NYF8 Q9NYF8-3; Q9NYF8; Q9NYF8-2 1 3 0.0983 0 1 1 928.4244 2783.25866 2783.26019 -0.55 -0.00051 3190487.25 CID (Collision Induced Dissociation) MS2 2.770479 40 99.5 24.539 103.6747 34052 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 98.6 104.2 77.7 101.0 93.8 102.1 114.1 112.3 95.7 96.0 Excluded by Method 7.12 1 0.0003003 7.258e-06 False High Sequest HT (A2) Unambiguous SPASDTYIVFGEAK [K].sPASDTYIVFGEAk.[I] N-Term(TMT6plex); K14(TMT6plex) 3 Q13765 Q13765; E9PAV3; E9PAV3-2 0 3 0.4671 0 1 1 648.356 1943.05345 1943.05261 0.43 0.00028 457479.375 CID (Collision Induced Dissociation) MS2 23.21296 50 50.4 60.0 102.0207 33433 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 55.1 49.0 42.4 48.0 48.7 49.7 53.7 45.1 61.6 50.4 Excluded by Method 3.04 1 0.0003003 7.278e-05 False High Sequest HT (A2) Unambiguous EEVSPEAVGVTSQRPVFCPFHK [K].eEVsPEAVGVTSQRPVFcPFHk.[K] N-Term(TMT6plex); S4(Phospho); C18(Carbamidomethyl); K22(TMT6plex) 2 O15164 O15164-2; O15164 0 4 0.5401 0 1 1 760.38528 3038.51929 3038.52111 -0.6 -0.00046 495952.28125 CID (Collision Induced Dissociation) MS2 47.18534 40 34.4 60.0 100.2197 32765 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 33.6 37.6 30.6 34.1 24.7 34.2 34.6 36.8 44.8 33.1 Excluded by Method 5.98 1 0.0003003 5.883e-06 False High Sequest HT (A2) Unambiguous IEEVLSPEGSPSKSPSK [K].iEEVLSPEGsPskSPSk.[K] N-Term(TMT6plex); S10(Phospho); S12(Phospho); K13(TMT6plex); K17(TMT6plex) 2 Q9UEY8 Q9UEY8-2; Q9UEY8 1 4 0.0167 0 1 1 655.3386 2618.33255 2618.33344 -0.34 -0.00022 266487.0 CID (Collision Induced Dissociation) MS2 9.680576 40 154.5 60.0 99.9972 32681 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 147.8 154.2 111.8 194.2 139.7 135.3 193.0 161.3 162.7 145.3 Excluded by Method 4.79 1 0.0003003 5.27e-05 False High Sequest HT (A2) Unambiguous NFNYNNNLQGYQAGFQPQSQGMSLNDFQK [K].nFNYNNNLQGYQAGFQPQSQGmSLNDFQk.[Q] N-Term(TMT6plex); M22(Oxidation); K29(TMT6plex) 1 P05453 P05453 0 4 0.5508 0 1 1 957.4686 3826.85258 3826.82839 6.32 0.00605 700601.6875 CID (Collision Induced Dissociation) MS2 0.0 50 42.2 60.0 99.8626 32631 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 11.1 32.3 61.5 17.9 43.3 79.2 17.6 36.4 84.7 38.4 Excluded by Method 5.9 1 0.0003003 3.842e-05 False High Sequest HT (A2) Unambiguous IEEVLSPEGSPSK [K].iEEVLSPEGsPSk.[S] N-Term(TMT6plex); S10(Phospho); K13(TMT6plex) 2 Q9UEY8 Q9UEY8-2; Q9UEY8 0 3 0.1556 0 1 1 637.336 1909.99346 1909.9924 0.55 0.00035 653944.0 CID (Collision Induced Dissociation) MS2 10.30912 30 249.0 60.0 99.3111 32426 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 244.4 227.2 193.9 266.2 235.2 242.6 305.8 274.6 249.2 251.4 Excluded by Method 4.82 1 0.0003003 0.0001757 False High Sequest HT (A2) Unambiguous DIDSEDEQDPFESHFNQVPEK [K].dIDsEDEQDPFESHFNQVPEk.[F] N-Term(TMT6plex); S4(Phospho); K21(TMT6plex) 1 P40498 P40498 0 3 0.357 0 1 1 1015.12603 3043.36353 3043.36103 0.82 0.00083 299740.3125 CID (Collision Induced Dissociation) MS2 0.0 30 73.0 60.0 101.6852 33312 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 16.6 58.9 117.1 29.8 63.3 129.0 26.5 71.6 145.9 71.5 Excluded by Method 5.77 1 0.0003003 6.051e-05 False High Sequest HT (A2) Unambiguous FEGSDDDENHPLPPLNVAK [K].fEGsDDDENHPLPPLNVAk.[G] N-Term(TMT6plex); S4(Phospho); K19(TMT6plex) 1 P13185 P13185 0 3 0.6298 0 1 1 878.09454 2632.26906 2632.26963 -0.22 -0.00019 687451.5 CID (Collision Induced Dissociation) MS2 0.0 40 75.6 60.0 101.2383 33144 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 23.2 63.8 108.2 22.1 58.0 139.1 25.3 77.7 142.6 95.7 Excluded by Method 6.51 1 0.0003003 6.217e-07 False High Sequest HT (A2) Unambiguous VQGEAVSNIQENTQTPTVQEESEEEEVDETGVEVK [K].vQGEAVSNIQENTQTPTVQEEsEEEEVDETGVEVk.[D] N-Term(TMT6plex); S22(Phospho); K35(TMT6plex) 3 Q13765 Q13765; E9PAV3; E9PAV3-2 0 4 0.0361 0 1 1 1100.52083 4399.0615 4399.06016 0.3 0.00033 1799303.0 CID (Collision Induced Dissociation) MS2 0.0 50 73.6 30.631 100.9375 33029 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 76.4 76.4 60.0 71.1 68.9 72.2 94.2 68.7 73.5 74.3 Excluded by Method 6.93 1 0.0003003 5.832e-07 False High Sequest HT (A2) Unambiguous GFSVVADTPELQRIK [K].gFSVVADtPELQRIk.[K] N-Term(TMT6plex); T8(Phospho); K15(TMT6plex) 3 Q14847 Q14847-2; Q14847-3; Q14847 1 3 0.1473 0 1 1 733.40529 2198.20131 2198.19864 1.21 0.00089 963564.3125 CID (Collision Induced Dissociation) MS2 0.0 30 195.4 60.0 104.0996 34211 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 209.1 204.7 177.0 174.6 189.7 192.4 217.3 199.4 199.3 190.3 Excluded by Method 2.24 1 0.0003003 0.0004999 False High Sequest HT (A2) Unambiguous LAAVPAAGPASAGGAAAASGDAAAEEEKEEEAAEESDDDMGFGLFD [K].lAAVPAAGPASAGGAAAASGDAAAEEEkEEEAAEEsDDDmGFGLFD.[-] N-Term(TMT6plex); K28(TMT6plex); S36(Phospho); M40(Oxidation) 1 P05319 P05319 1 5 0.0588 0 1 1 985.24589 4922.20037 4922.17633 4.88 0.00481 3011267.5 CID (Collision Induced Dissociation) MS2 19.77066 60 21.3 25.257 127.5352 42924 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 3.7 14.6 26.8 9.2 19.2 41.4 8.1 19.6 43.6 26.7 Excluded by Method 4.25 1 0.0003003 0.0001263 False High Sequest HT (A2) Unambiguous AILESVGIEIEDEK [K].aILESVGIEIEDEk.[V] N-Term(TMT6plex); K14(TMT6plex) 1 P05319 P05319 0 3 0.2523 0 1 1 668.38191 2003.13117 2003.13126 -0.05 -3e-05 1041067.25 CID (Collision Induced Dissociation) MS2 34.21291 60 47.3 60.0 127.4038 42867 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 19.3 35.3 63.3 18.8 41.4 88.7 22.1 45.8 93.7 45.0 Excluded by Method 3.21 1 0.0003003 0.0003697 False High Sequest HT (A2) Unambiguous FTPVNITSPNLSFQK [K].fTPVNITsPNLSFQk.[R] N-Term(TMT6plex); S8(Phospho); K15(TMT6plex) 1 Q06685 Q06685 0 3 0.0667 0 1 1 744.40081 2231.18788 2231.18774 0.06 5e-05 3633364.0 CID (Collision Induced Dissociation) MS2 0.0 40 42.1 21.817 127.3075 42823 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 15.4 35.2 62.6 15.6 30.7 79.9 18.2 35.0 85.4 43.1 Excluded by Method 3.0 1 0.0003003 0.0005516 False High Sequest HT (A2) Unambiguous APLATGEDDDDEVPDLVENFDEASK [K].aPLATGEDDDDEVPDLVENFDEASk.[N] N-Term(TMT6plex); K25(TMT6plex) 2 P20290 P20290-2; P20290 0 3 0.5643 0 1 1 1050.50383 3149.49695 3149.50503 -2.57 -0.0027 1675225.25 CID (Collision Induced Dissociation) MS2 24.78952 40 62.1 58.406 127.2057 42782 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 63.3 58.7 57.9 63.9 59.5 58.3 61.9 71.1 63.0 63.5 Excluded by Method 6.84 1 0.0003003 1.109e-07 False High Sequest HT (A2) Unambiguous EEEAAEESDDDMGFGLFD [K].eEEAAEEsDDDmGFGLFD.[-] N-Term(TMT6plex); S8(Phospho); M12(Oxidation) 2 P05319 P10622; P05319 0 2 0.808 0 1 1 1165.94112 2330.87497 2330.87332 0.71 0.00083 15546685.0 CID (Collision Induced Dissociation) MS2 6.046947 60 52.1 9.242 127.3598 42847 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 12.2 42.3 84.7 15.1 37.5 99.7 18.5 50.6 102.0 58.5 Excluded by Method 5.78 1 0.0003003 8.036e-06 False High Sequest HT (A2) Unambiguous ALVLELDEMSNGSEIQDALEEISGQK [K].aLVLELDEmsNGSEIQDALEEISGQk.[T] N-Term(TMT6plex); M9(Oxidation); S10(Phospho); K26(TMT6plex) 2 P17695 P17695; P17695-2 0 3 0.325 0 1 1 1124.88944 3372.65377 3372.65337 0.12 0.00013 28501462.0 CID (Collision Induced Dissociation) MS2 0.0 40 28.8 7.623 130.055 44073 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 7.5 24.5 46.0 10.4 20.3 45.7 14.9 21.1 66.4 30.9 Excluded by Method 2.4 1 0.0003003 0.0002054 False High Sequest HT (A2) Unambiguous NNSNDIVNAIMELTM [K].nNSNDIVNAImELTm.[-] N-Term(TMT6plex); M11(Oxidation); M15(Oxidation) 3 Q13765 Q13765; E9PAV3; E9PAV3-2 0 2 0.3716 0 1 1 970.46907 1939.93085 1939.93024 0.31 0.00031 4833869.5 CID (Collision Induced Dissociation) MS2 15.43657 50 79.5 18.474 129.7677 43946 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 69.5 82.0 65.9 86.7 72.5 87.2 80.6 78.0 85.9 86.5 Excluded by Method 3.66 1 0.0003003 4.304e-05 False High Sequest HT (A2) Unambiguous EEEAAEESDDDMGFGLFD [K].eEEAAEEsDDDmGFGLFD.[-] N-Term(TMT6plex); S8(Phospho); M12(Oxidation) 2 P05319 P10622; P05319 0 2 0.8008 0 1 1 1165.94222 2330.87717 2330.87332 1.65 0.00193 13220496.0 CID (Collision Induced Dissociation) MS2 39.2711 60 34.3 9.987 129.5949 43870 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 9.7 29.2 49.5 9.8 31.6 59.6 8.7 36.0 70.3 39.0 Excluded by Method 5.07 1 0.0003003 2.894e-05 False High Sequest HT (A2) Unambiguous YAAAAPAATSAASGDAAPAEEAAAEEEEESDDDMGFGLFD [K].yAAAAPAATSAASGDAAPAEEAAAEEEEEsDDDmGFGLFD.[-] N-Term(TMT6plex); S30(Phospho); M34(Oxidation) 1 P05317 P05317 0 4 0.3905 0 1 1 1062.18875 4245.73316 4245.73286 0.07 7e-05 9357024.0 CID (Collision Induced Dissociation) MS2 2.461923 70 49.5 8.097 129.4726 43817 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 9.6 46.4 77.3 12.6 34.1 102.7 16.7 49.9 101.0 44.5 5.71 1 0.0003003 1.705e-05 False High Sequest HT (A2) Unambiguous DSTLIMQLLRDNLTLWTSENQGDEGDAGEGEN [K].dSTLImQLLRDNLTLWTSENQGDEGDAGEGEN.[-] N-Term(TMT6plex); M6(Oxidation) 2 P31946 P31946-2; P31946 1 4 0.4713 0 1 1 942.44482 3766.75747 3766.75512 0.62 0.00059 1605540.375 CID (Collision Induced Dissociation) MS2 29.77886 40 20.4 17.914 129.1282 43664 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 19.1 23.0 18.3 21.5 16.5 19.0 21.3 19.2 19.8 26.2 Excluded by Method 5.05 1 0.0003003 0.0004274 False High Sequest HT (A2) Unambiguous EEEAAEESDDDMGFGLFD [K].eEEAAEEsDDDMGFGLFD.[-] N-Term(TMT6plex); S8(Phospho) 2 P05319 P10622; P05319 0 2 0.8292 0 1 1 1157.94272 2314.87817 2314.8784 -0.1 -0.00011 3955546.25 CID (Collision Induced Dissociation) MS2 16.55494 60 77.8 26.803 130.4748 44259 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 24.8 73.6 117.8 21.7 56.5 150.8 28.5 79.8 150.1 74.7 Excluded by Method 4.86 1 0.0003003 4.8e-07 False High Sequest HT (A2) Unambiguous DLVLPTQALPASPALK [K].dLVLPTQALPAsPALk.[N] N-Term(TMT6plex); S12(Phospho); K16(TMT6plex) 1 Q15554 Q15554 0 3 0.4179 0 1 1 724.75308 2172.24469 2172.24453 0.07 5e-05 10161043.0 CID (Collision Induced Dissociation) MS2 0.7743405 40 111.1 10.6 127.0181 42702 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 120.1 114.5 97.0 121.3 101.0 107.2 120.4 107.2 106.4 115.5 Excluded by Method 4.14 1 0.0003003 4.752e-06 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVssDEEF.[-] N-Term(TMT6plex); S8(Phospho); S9(Phospho) 1 P16521 P16521 0 2 0.1269 0 1 1 925.35483 1849.70238 1849.70195 0.23 0.00021 13651265.0 CID (Collision Induced Dissociation) MS2 9.817993 40 141.2 13.96 126.7373 42596 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 28.3 120.7 241.1 32.0 113.1 291.8 43.2 132.0 271.4 138.8 Excluded by Method 4.57 1 0.0003003 5.799e-05 False High Sequest HT (A2) Unambiguous SFLVIVDGSPK [K].sFLVIVDGsPk.[N] N-Term(TMT6plex); S9(Phospho); K11(TMT6plex) 2 Q9H116 Q9H116; Q9H116-4 0 3 0.6244 0 1 1 567.31965 1699.9444 1699.9436 0.47 0.00027 680604.375 CID (Collision Induced Dissociation) MS2 0.0 60 34.6 60.0 124.5947 41800 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 36.6 31.2 22.7 40.8 33.7 35.9 37.4 34.8 40.0 33.1 Excluded by Method 3.86 1 0.0003003 2.318e-05 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVssDEEF.[-] N-Term(TMT6plex); S8(Phospho); S9(Phospho) 1 P16521 P16521 0 2 0.121 0 1 1 925.35489 1849.7025 1849.70195 0.3 0.00027 16585658.0 CID (Collision Induced Dissociation) MS2 1.554277 40 209.5 13.673 124.4803 41757 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 45.1 158.2 359.5 55.9 169.2 415.0 69.1 190.6 426.1 205.8 Excluded by Method 4.71 1 0.0003003 4.575e-05 False High Sequest HT (A2) Unambiguous LALSPLSLGK [K].lALsPLSLGk.[E] N-Term(TMT6plex); S4(Phospho); K10(TMT6plex) 1 P49454 P49454 0 2 0.0932 0 1 1 768.96198 1536.91669 1536.91665 0.03 2e-05 875931.5 CID (Collision Induced Dissociation) MS2 39.13133 40 140.2 60.0 124.4132 41733 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 155.4 153.3 122.2 128.3 133.2 139.7 156.1 159.4 129.6 124.8 Excluded by Method 3.97 1 0.0003003 0.0002169 False High Sequest HT (A2) Unambiguous GSGEDDEEETETTPTSTVPVATIAQETLK [K].gSGEDDEEETETtPTSTVPVATIAQETLk.[V] N-Term(TMT6plex); T13(Phospho); K29(TMT6plex) 1 P12962 P12962 0 3 0.0404 0 1 1 1191.90299 3573.69442 3573.69846 -1.13 -0.00135 472754.65625 CID (Collision Induced Dissociation) MS2 0.0 40 88.8 60.0 124.2326 41670 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 21.8 68.6 139.9 28.3 63.3 171.2 29.8 88.6 173.5 103.5 Excluded by Method 5.7 1 0.0003003 5.344e-07 False High Sequest HT (A2) Unambiguous FVQGLLQNAAANAEAK [K].fVQGLLQNAAANAEAk.[G] N-Term(TMT6plex); K16(TMT6plex) 2 P46990 P46990; P05740 0 3 0.4847 0 1 1 701.73664 2103.19538 2103.19626 -0.42 -0.00029 1028432.3125 CID (Collision Induced Dissociation) MS2 15.32676 40 59.8 60.0 124.144 41636 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 15.0 46.8 87.5 20.0 49.7 113.8 21.5 59.7 120.2 64.1 Excluded by Method 4.89 1 0.0003003 7.403e-07 False High Sequest HT (A2) Unambiguous ITEIEDGQDGGVTPYSNESGLVNADLDVK [K].iTEIEDGQDGGVtPYSNESGLVNADLDVk.[D] N-Term(TMT6plex); T13(Phospho); K29(TMT6plex) 1 P04050 P04050 0 3 0.0361 0 1 1 1191.91337 3573.72555 3573.72495 0.17 0.0002 757817.5625 CID (Collision Induced Dissociation) MS2 0.0 60 34.7 60.0 125.4398 42111 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 12.2 23.6 54.3 10.6 30.4 54.7 9.5 36.9 79.4 35.8 Excluded by Method 7.75 1 0.0003003 5.749e-08 False High Sequest HT (A2) Unambiguous LSLGQYDNDAGGQLPFSK [K].lsLGQYDNDAGGQLPFSk.[C] N-Term(TMT6plex); S2(Phospho); K18(TMT6plex) 2 Q68CZ2 Q68CZ2; Q68CZ2-2 0 3 0.0493 0 1 1 816.74571 2448.22258 2448.22123 0.55 0.00045 1410593.25 CID (Collision Induced Dissociation) MS2 77.16142 40 77.2 43.854 126.6132 42550 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 70.7 64.3 74.4 72.5 76.1 84.9 91.4 72.3 82.2 83.2 Excluded by Method 3.65 1 0.0003003 0.0003179 False High Sequest HT (A2) Unambiguous AEDDTGEEEEDDPVIEEFPLK [K].aEDDtGEEEEDDPVIEEFPLk.[I] N-Term(TMT6plex); T5(Phospho); K21(TMT6plex) 1 P36121 P36121 0 3 0.5725 0 1 1 982.11223 2944.32215 2944.32766 -1.87 -0.00184 848902.3125 CID (Collision Induced Dissociation) MS2 0.0 40 25.6 60.0 126.3726 42461 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 20.2 21.1 26.5 14.9 25.2 35.1 27.6 26.9 29.8 29.0 Excluded by Method 4.14 1 0.0003003 0.0003114 False High Sequest HT (A2) Unambiguous VQGEAVSNIQENTQTPTVQEESEEEEVDETGVEVK [K].vQGEAVSNIQENTQTPTVQEEsEEEEVDETGVEVk.[D] N-Term(TMT6plex); S22(Phospho); K35(TMT6plex) 3 Q13765 Q13765; E9PAV3; E9PAV3-2 0 4 0.0281 0 1 1 1100.52034 4399.05955 4399.06016 -0.14 -0.00015 448182.71875 CID (Collision Induced Dissociation) MS2 0.0 40 11.6 60.0 126.0274 42331 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 15.9 11.9 9.9 7.1 8.2 12.0 15.1 12.2 10.1 14.0 Excluded by Method 4.27 1 0.0003003 4.108e-05 False High Sequest HT (A2) Unambiguous FLYGSDSELALPSSK [K].fLYGsDSELALPSSk.[R] N-Term(TMT6plex); S5(Phospho); K15(TMT6plex) 1 P45976 P45976 0 3 0.011 0 1 1 718.03782 2152.09891 2152.09792 0.46 0.00033 736019.5 CID (Collision Induced Dissociation) MS2 9.374095 20 322.5 60.0 125.8288 42258 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 69.7 254.8 554.9 92.1 264.9 599.6 111.8 327.5 635.3 314.8 Excluded by Method 4.56 1 0.0003003 6.78e-06 False High Sequest HT (A2) Unambiguous EEVTEGQAEATEWAEENADNVEW [K].eEVTEGQAEATEWAEENADNVEW.[-] N-Term(TMT6plex) 1 P46654 P46654 0 3 0.6313 0 1 1 955.7551 2865.25076 2865.25362 -1.0 -0.00095 1066465.0 CID (Collision Induced Dissociation) MS2 15.57861 80 61.6 60.0 125.7559 42231 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 16.3 47.9 92.1 12.6 48.6 139.7 20.0 58.0 119.8 61.0 5.18 1 0.0003003 6.769e-07 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVssDEEF.[-] N-Term(TMT6plex); S8(Phospho); S9(Phospho) 1 P16521 P16521 0 2 0.1294 0 1 1 925.35458 1849.70189 1849.70195 -0.03 -3e-05 14784415.0 CID (Collision Induced Dissociation) MS2 2.713817 40 223.7 16.957 125.6002 42171 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 49.6 182.8 380.7 56.5 174.3 420.9 77.9 207.8 461.9 224.9 Excluded by Method 4.56 1 0.0003003 8.961e-05 False High Sequest HT (A2) Unambiguous EEEAAEESDDDMGFGLFD [K].eEEAAEEsDDDmGFGLFD.[-] N-Term(TMT6plex); S8(Phospho); M12(Oxidation) 2 P05319 P10622; P05319 0 2 0.79 0 1 1 1165.94588 2330.88449 2330.87332 4.79 0.00559 2186701.75 CID (Collision Induced Dissociation) MS2 0.0 60 81.3 60.0 125.8691 42273 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 23.2 60.1 123.5 25.4 63.1 171.1 28.0 66.8 172.4 79.8 Excluded by Method 5.38 1 0.0003003 3.432e-06 False High Sequest HT (A2) Unambiguous DSTLIMQLLRDNLTLWTSENQGDEGDAGEGEN [K].dSTLImQLLRDNLTLWTSENQGDEGDAGEGEN.[-] N-Term(TMT6plex); M6(Oxidation) 2 P31946 P31946-2; P31946 1 4 0.6334 0 1 1 942.44458 3766.75649 3766.75512 0.36 0.00034 5054440.5 CID (Collision Induced Dissociation) MS2 4.424623 0 19.4 27.576 130.6356 44329 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 19.1 18.8 18.7 14.3 16.1 24.5 24.4 18.3 23.6 16.3 Excluded by Method 6.52 1 0.0003003 3.211e-07 False High Sequest HT (A2) Unambiguous ALVLELDEMSNGSEIQDALEEISGQK [K].aLVLELDEmsNGSEIQDALEEISGQk.[T] N-Term(TMT6plex); M9(Oxidation); S10(Phospho); K26(TMT6plex) 2 P17695 P17695; P17695-2 0 4 0.0757 0 1 1 843.92035 3372.65955 3372.65337 1.83 0.00155 267788.96875 CID (Collision Induced Dissociation) MS2 5.890699 60 44.2 60.0 142.1787 48551 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 15.3 32.4 59.4 16.0 35.0 86.1 24.7 38.2 93.1 41.7 Excluded by Method 5.15 1 0.0003003 0.0003744 False High Sequest HT (A2) Unambiguous YAAAAPAATSAASGDAAPAEEAAAEEEEESDDDMGFGLFD [K].yAAAAPAATSAASGDAAPAEEAAAEEEEEsDDDmGFGLFD.[-] N-Term(TMT6plex); S30(Phospho); M34(Oxidation) 1 P05317 P05317 0 4 0.3275 0 1 1 1062.19058 4245.74049 4245.73286 1.8 0.00191 535042.5 CID (Collision Induced Dissociation) MS2 5.043761 50 27.3 60.0 141.2196 48218 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 9.1 20.6 41.6 4.0 19.4 58.3 11.5 25.3 53.0 29.8 Excluded by Method 4.58 1 0.0003003 0.0005022 False High Sequest HT (A2) Unambiguous EEEAAEESDDDMGFGLFD [K].eEEAAEEsDDDMGFGLFD.[-] N-Term(TMT6plex); S8(Phospho) 2 P05319 P10622; P05319 0 3 0.7925 0 1 1 772.29798 2314.87938 2314.8784 0.42 0.00033 775925.75 CID (Collision Induced Dissociation) MS2 0.0 60 33.9 60.0 140.7964 48068 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 12.6 31.3 46.6 7.9 25.2 65.7 13.7 35.0 67.3 33.9 Excluded by Method 4.82 1 0.0003003 1.776e-05 False High Sequest HT (A2) Unambiguous EEEAAEESDDDMGFGLFD [K].eEEAAEEsDDDMGFGLFD.[-] N-Term(TMT6plex); S8(Phospho) 2 P05319 P10622; P05319 0 2 0.6371 0 1 1 1157.94138 2314.87549 2314.8784 -1.26 -0.00146 554234.3125 CID (Collision Induced Dissociation) MS2 63.70475 60 20.1 60.0 140.2824 47888 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 8.2 15.1 27.5 3.0 18.9 35.4 7.5 19.6 49.0 17.0 Excluded by Method 3.61 1 0.0003003 4.493e-06 False High Sequest HT (A2) Unambiguous EEEAAEESDDDMGFGLFD [K].eEEAAEEsDDDMGFGLFD.[-] N-Term(TMT6plex); S8(Phospho) 2 P05319 P10622; P05319 0 3 0.8166 0 1 1 772.29743 2314.87773 2314.8784 -0.29 -0.00022 503576.15625 CID (Collision Induced Dissociation) MS2 6.388542 60 34.1 60.0 139.7522 47702 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 10.9 30.2 48.1 10.9 27.1 63.9 12.9 32.4 67.5 37.0 Excluded by Method 4.69 1 0.0003003 0.0004165 False High Sequest HT (A2) Unambiguous ALVLELDEMSNGSEIQDALEEISGQK [K].aLVLELDEmsNGSEIQDALEEISGQk.[T] N-Term(TMT6plex); M9(Oxidation); S10(Phospho); K26(TMT6plex) 2 P17695 P17695; P17695-2 0 4 0.0719 0 1 1 843.92218 3372.66688 3372.65337 4.0 0.00338 387086.78125 CID (Collision Induced Dissociation) MS2 19.93258 50 34.0 60.0 139.2777 47538 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 13.8 28.1 50.7 10.4 24.6 64.6 18.6 34.1 61.3 33.9 Excluded by Method 4.87 1 0.0003003 0.0002944 False High Sequest HT (A2) Unambiguous EEEAAEESDDDMGFGLFD [K].eEEAAEEsDDDMGFGLFD.[-] N-Term(TMT6plex); S8(Phospho) 2 P05319 P10622; P05319 0 3 0.8363 0 1 1 772.29731 2314.87737 2314.8784 -0.45 -0.00035 788122.1875 CID (Collision Induced Dissociation) MS2 0.0 80 41.7 60.0 138.4684 47256 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 12.1 33.8 64.7 15.0 27.4 77.0 15.9 45.0 78.0 48.5 3.91 1 0.0003003 8.898e-05 False High Sequest HT (A2) Unambiguous ALVLELDEMSNGSEIQDALEEISGQK [K].aLVLELDEmsNGSEIQDALEEISGQk.[T] N-Term(TMT6plex); M9(Oxidation); S10(Phospho); K26(TMT6plex) 2 P17695 P17695; P17695-2 0 4 0.0642 0 1 1 843.92169 3372.66492 3372.65337 3.43 0.00289 593259.0625 CID (Collision Induced Dissociation) MS2 26.34495 60 43.3 60.0 138.3292 47207 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 19.8 39.3 67.3 15.7 27.6 82.1 23.1 36.2 85.0 37.2 Excluded by Method 4.83 1 0.0003003 0.0002829 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVssDEEF.[-] N-Term(TMT6plex); S8(Phospho); S9(Phospho) 1 P16521 P16521 0 2 0.1574 0 1 1 925.35556 1849.70385 1849.70195 1.02 0.00095 600160.25 CID (Collision Induced Dissociation) MS2 21.81759 50 67.3 60.0 138.2613 47183 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 14.2 58.2 119.5 22.5 54.8 116.8 23.3 57.2 137.7 68.5 Excluded by Method 3.24 1 0.0003003 0.000501 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVssDEEF.[-] N-Term(TMT6plex); S8(Phospho); S9(Phospho) 1 P16521 P16521 0 2 0.1246 0 1 1 925.35422 1849.70116 1849.70195 -0.43 -0.0004 722723.0 CID (Collision Induced Dissociation) MS2 19.31643 40 72.0 60.0 142.5312 48671 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 16.4 66.8 119.8 20.9 52.3 145.0 23.3 70.7 134.9 69.5 Excluded by Method 3.13 1 0.0003003 0.0002617 False High Sequest HT (A2) Unambiguous YAAAAPAATSAASGDAAPAEEAAAEEEEESDDDMGFGLFD [K].yAAAAPAATSAASGDAAPAEEAAAEEEEEsDDDmGFGLFD.[-] N-Term(TMT6plex); S30(Phospho); M34(Oxidation) 1 P05317 P05317 0 4 0.4867 0 1 1 1062.19204 4245.74635 4245.73286 3.18 0.00337 337118.09375 CID (Collision Induced Dissociation) MS2 12.46127 50 10.5 60.0 148.4264 50723 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 9.6 14.5 9.8 21.1 2.9 11.7 23.2 11.8 Excluded by Method 4.89 1 0.0003003 0.0002298 False High Sequest HT (A2) Unambiguous EEEAAEESDDDMGFGLFD [K].eEEAAEEsDDDmGFGLFD.[-] N-Term(TMT6plex); S8(Phospho); M12(Oxidation) 2 P05319 P10622; P05319 0 2 0.6511 0 1 1 1165.9443 2330.88132 2330.87332 3.43 0.004 809613.0625 CID (Collision Induced Dissociation) MS2 0.0 70 46.5 60.0 147.8906 50538 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 12.4 39.7 68.5 16.0 37.5 91.0 18.5 43.2 88.2 50.4 4.07 1 0.0003003 0.0002514 False High Sequest HT (A2) Unambiguous EEEAAEESDDDMGFGLFD [K].eEEAAEEsDDDMGFGLFD.[-] N-Term(TMT6plex); S8(Phospho) 2 P05319 P10622; P05319 0 3 0.7761 0 1 1 772.29889 2314.88213 2314.8784 1.61 0.00124 245104.9375 CID (Collision Induced Dissociation) MS2 10.1269 70 20.6 60.0 147.4856 50398 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 3.7 18.6 35.1 7.0 12.8 47.3 7.0 14.9 39.1 20.7 4.02 1 0.0003003 1.944e-05 False High Sequest HT (A2) Unambiguous YAAAAPAATSAASGDAAPAEEAAAEEEEESDDDMGFGLFD [K].yAAAAPAATSAASGDAAPAEEAAAEEEEEsDDDmGFGLFD.[-] N-Term(TMT6plex); S30(Phospho); M34(Oxidation) 1 P05317 P05317 0 4 0.3682 0 1 1 1062.19131 4245.74342 4245.73286 2.49 0.00264 451951.40625 CID (Collision Induced Dissociation) MS2 15.30571 50 12.5 60.0 147.349 50350 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 1.4 10.1 21.6 4.8 9.6 22.3 2.0 13.1 26.7 12.9 Excluded by Method 4.97 1 0.0003003 0.0001352 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVssDEEF.[-] N-Term(TMT6plex); S8(Phospho); S9(Phospho) 1 P16521 P16521 0 2 0.1271 0 1 1 925.35495 1849.70262 1849.70195 0.36 0.00034 775687.625 CID (Collision Induced Dissociation) MS2 20.83553 50 87.7 60.0 146.7875 50154 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 24.8 69.3 147.4 26.7 65.7 179.6 34.8 81.7 169.0 78.3 Excluded by Method 3.62 1 0.0003003 0.0003229 False High Sequest HT (A2) Unambiguous EEEAAEESDDDMGFGLFD [K].eEEAAEEsDDDMGFGLFD.[-] N-Term(TMT6plex); S8(Phospho) 2 P05319 P10622; P05319 0 3 0.7817 0 1 1 772.2984 2314.88066 2314.8784 0.98 0.00075 426742.9375 CID (Collision Induced Dissociation) MS2 8.996144 80 34.5 60.0 142.9214 48807 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 7.5 32.2 44.8 14.4 25.7 73.3 12.7 30.1 65.9 38.6 4.49 1 0.0003003 3.595e-05 False High Sequest HT (A2) Unambiguous EEEAAEESDDDMGFGLFD [K].eEEAAEEsDDDMGFGLFD.[-] N-Term(TMT6plex); S8(Phospho) 2 P05319 P10622; P05319 0 3 0.8062 0 1 1 772.29804 2314.87956 2314.8784 0.5 0.00039 251035.484375 CID (Collision Induced Dissociation) MS2 9.738035 80 25.9 60.0 146.3518 50002 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 3.7 24.6 33.4 9.6 24.7 44.0 10.9 31.8 47.0 29.1 4.49 1 0.0003003 0.0001072 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVssDEEF.[-] N-Term(TMT6plex); S8(Phospho); S9(Phospho) 1 P16521 P16521 0 2 0.1424 0 1 1 925.35495 1849.70262 1849.70195 0.36 0.00034 1076792.25 CID (Collision Induced Dissociation) MS2 22.59525 40 85.0 60.0 145.7254 49781 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 19.0 71.9 138.6 22.8 70.1 160.7 30.3 80.5 174.7 81.2 Excluded by Method 3.44 1 0.0003003 0.0004951 False High Sequest HT (A2) Unambiguous EEEAAEESDDDMGFGLFD [K].eEEAAEEsDDDMGFGLFD.[-] N-Term(TMT6plex); S8(Phospho) 2 P05319 P10622; P05319 0 3 0.7962 0 1 1 772.29743 2314.87773 2314.8784 -0.29 -0.00022 422058.03125 CID (Collision Induced Dissociation) MS2 0.0 60 24.0 60.0 145.2128 49600 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 3.1 20.2 39.6 2.3 17.3 47.1 10.0 25.3 49.8 25.7 Excluded by Method 4.71 1 0.0003003 6.117e-06 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVssDEEF.[-] N-Term(TMT6plex); S8(Phospho); S9(Phospho) 1 P16521 P16521 0 2 0.1558 0 1 1 925.35507 1849.70287 1849.70195 0.49 0.00046 852503.125 CID (Collision Induced Dissociation) MS2 6.217478 40 80.3 60.0 144.6639 49409 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 16.5 59.4 128.4 18.4 58.8 166.4 31.2 71.5 164.5 87.9 Excluded by Method 3.53 1 0.0003003 0.0002663 False High Sequest HT (A2) Unambiguous EEEAAEESDDDMGFGLFD [K].eEEAAEEsDDDMGFGLFD.[-] N-Term(TMT6plex); S8(Phospho) 2 P05319 P10622; P05319 0 3 0.7804 0 1 1 772.29731 2314.87737 2314.8784 -0.45 -0.00035 405570.34375 CID (Collision Induced Dissociation) MS2 7.685398 60 28.4 60.0 144.0441 49199 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 7.6 30.5 42.2 7.9 18.0 47.8 11.1 25.9 59.4 33.8 Excluded by Method 3.78 1 0.0003003 4.985e-05 False High Sequest HT (A2) Unambiguous YAAAAPAATSAASGDAAPAEEAAAEEEEESDDDMGFGLFD [K].yAAAAPAATSAASGDAAPAEEAAAEEEEEsDDDmGFGLFD.[-] N-Term(TMT6plex); S30(Phospho); M34(Oxidation) 1 P05317 P05317 0 4 0.3702 0 1 1 1062.19534 4245.75953 4245.73286 6.28 0.00667 492692.21875 CID (Collision Induced Dissociation) MS2 3.462368 40 14.9 60.0 145.913 49848 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 6.6 12.5 23.3 12.0 29.7 7.5 13.1 32.0 11.8 Excluded by Method 4.7 1 0.0003003 0.0002795 False High Sequest HT (A2) Unambiguous YAAAAPAATSAASGDAAPAEEAAAEEEEESDDDMGFGLFD [K].yAAAAPAATSAASGDAAPAEEAAAEEEEEsDDDmGFGLFD.[-] N-Term(TMT6plex); S30(Phospho); M34(Oxidation) 1 P05317 P05317 0 4 0.3557 0 1 1 1062.19388 4245.75367 4245.73286 4.9 0.0052 730150.875 CID (Collision Induced Dissociation) MS2 0.0 50 19.5 60.0 137.4672 46904 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 3.9 16.5 31.1 6.4 14.0 36.2 8.2 18.1 36.3 24.0 Excluded by Method 4.92 1 0.0003003 0.0001834 False High Sequest HT (A2) Unambiguous ALVLELDEMSNGSEIQDALEEISGQK [K].aLVLELDEmsNGSEIQDALEEISGQk.[T] N-Term(TMT6plex); M9(Oxidation); S10(Phospho); K26(TMT6plex) 2 P17695 P17695; P17695-2 0 4 0.0455 0 1 1 843.9201 3372.65858 3372.65337 1.54 0.0013 799217.625 CID (Collision Induced Dissociation) MS2 15.60625 90 29.3 35.21 132.5233 45124 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 12.2 27.1 37.5 13.0 16.2 57.5 18.8 26.7 51.1 32.5 4.62 1 0.0003003 0.0001397 False High Sequest HT (A2) Unambiguous EEEAAEESDDDMGFGLFD [K].eEEAAEEsDDDMGFGLFD.[-] N-Term(TMT6plex); S8(Phospho) 2 P05319 P10622; P05319 0 3 0.7411 0 1 1 772.29725 2314.87718 2314.8784 -0.53 -0.00041 2357369.75 CID (Collision Induced Dissociation) MS2 42.64081 60 89.5 41.612 131.6011 44748 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 61.6 95.6 110.1 52.2 71.9 128.8 67.2 93.4 126.0 88.1 Excluded by Method 4.79 1 0.0003003 5.669e-06 False High Sequest HT (A2) Unambiguous FALGQGVGVILCIGETLEEK [K].fALGQGVGVILcIGETLEEk.[K] N-Term(TMT6plex); C12(Carbamidomethyl); K20(TMT6plex) 1 P00942 P00942 0 3 0.5128 0 1 1 864.4894 2591.45365 2591.45188 0.68 0.00059 9847156.0 CID (Collision Induced Dissociation) MS2 1.587624 90 45.2 11.025 131.7391 44806 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 11.8 45.3 62.2 18.9 35.0 82.0 18.1 44.6 87.6 46.1 3.51 1 0.0003003 0.0002199 False High Sequest HT (A2) Unambiguous EEEAAEESDDDMGFGLFD [K].eEEAAEEsDDDMGFGLFD.[-] N-Term(TMT6plex); S8(Phospho) 2 P05319 P10622; P05319 0 3 0.732 0 1 1 772.29877 2314.88176 2314.8784 1.45 0.00112 451886.5 CID (Collision Induced Dissociation) MS2 0.0 80 36.6 60.0 137.4216 46888 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 10.5 34.3 56.5 10.3 29.9 75.3 13.4 31.2 69.2 35.0 3.88 1 0.0003003 5.958e-05 False High Sequest HT (A2) Unambiguous ALVLELDEMSNGSEIQDALEEISGQK [K].aLVLELDEmsNGSEIQDALEEISGQk.[T] N-Term(TMT6plex); M9(Oxidation); S10(Phospho); K26(TMT6plex) 2 P17695 P17695; P17695-2 0 4 0.0851 0 1 1 843.91949 3372.65613 3372.65337 0.82 0.00069 601016.9375 CID (Collision Induced Dissociation) MS2 16.78567 50 43.0 60.0 137.2023 46811 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 17.0 36.0 66.2 18.0 32.3 76.0 23.8 40.3 78.8 41.6 Excluded by Method 5.29 1 0.0003003 6.617e-05 False High Sequest HT (A2) Unambiguous VISAGRIIYVDDGVLSFQVLEVVDDK [K].vISAGRIIYVDDGVLSFQVLEVVDDk.[T] N-Term(TMT6plex); K26(TMT6plex) 1 P00549 P00549 1 4 0.4218 0 1 1 827.71963 3307.85669 3307.85536 0.4 0.00033 280463.15625 CID (Collision Induced Dissociation) MS2 34.624 40 11.1 60.0 136.672 46625 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 2.2 8.3 17.4 2.3 11.9 19.8 2.7 11.8 24.4 10.6 Excluded by Method 3.39 1 0.0003003 0.0005249 False High Sequest HT (A2) Unambiguous YAAAAPAATSAASGDAAPAEEAAAEEEEESDDDMGFGLFD [K].yAAAAPAATSAASGDAAPAEEAAAEEEEEsDDDmGFGLFD.[-] N-Term(TMT6plex); S30(Phospho); M34(Oxidation) 1 P05317 P05317 0 4 0.3362 0 1 1 1062.19436 4245.75562 4245.73286 5.36 0.00569 674202.375 CID (Collision Induced Dissociation) MS2 12.34918 40 22.2 60.0 136.3437 46509 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 2.7 19.9 39.2 6.8 16.6 44.7 7.9 20.6 40.8 22.3 Excluded by Method 4.64 1 0.0003003 0.0001639 False High Sequest HT (A2) Unambiguous EEEAAEESDDDMGFGLFD [K].eEEAAEEsDDDMGFGLFD.[-] N-Term(TMT6plex); S8(Phospho) 2 P05319 P10622; P05319 0 3 0.819 0 1 1 772.29798 2314.87938 2314.8784 0.42 0.00033 589913.0625 CID (Collision Induced Dissociation) MS2 6.773708 60 40.6 60.0 136.3414 46508 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 14.1 34.1 64.9 14.3 31.6 75.3 15.9 41.8 75.2 39.1 Excluded by Method 5.8 1 0.0003003 9.89e-07 False High Sequest HT (A2) Unambiguous VQGEAVSNIQENTQTPTVQEESEEEEVDETGVEVK [K].vQGEAVSNIQENTQTPTVQEEsEEEEVDETGVEVk.[D] N-Term(TMT6plex); S22(Phospho); K35(TMT6plex) 3 Q13765 Q13765; E9PAV3; E9PAV3-2 0 4 0.052 0 1 1 1100.52071 4399.06101 4399.06016 0.19 0.00021 141565.765625 CID (Collision Induced Dissociation) MS2 12.15865 40 15.1 60.0 135.8967 46356 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 11.7 14.8 13.2 14.6 13.9 14.4 17.7 21.5 14.5 14.5 Excluded by Method 5.0 1 0.0003003 5.374e-06 False High Sequest HT (A2) Unambiguous ALVLELDEMSNGSEIQDALEEISGQK [K].aLVLELDEmsNGSEIQDALEEISGQk.[T] N-Term(TMT6plex); M9(Oxidation); S10(Phospho); K26(TMT6plex) 2 P17695 P17695; P17695-2 0 3 0.0556 0 1 1 1124.89298 3372.66439 3372.65337 3.27 0.00367 494598.84375 CID (Collision Induced Dissociation) MS2 8.839705 90 25.9 60.0 135.569 46244 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 10.2 20.1 41.1 11.1 16.5 44.8 13.8 25.3 48.1 28.3 3.06 1 0.0003003 0.0002531 False High Sequest HT (A2) Unambiguous EEEAAEESDDDMGFGLFD [K].eEEAAEEsDDDMGFGLFD.[-] N-Term(TMT6plex); S8(Phospho) 2 P05319 P10622; P05319 0 3 0.748 0 1 1 772.29804 2314.87956 2314.8784 0.5 0.00039 1247010.875 CID (Collision Induced Dissociation) MS2 9.906731 50 35.2 60.0 135.2436 46128 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 20.7 30.1 47.3 18.9 23.6 60.6 22.5 31.2 63.8 33.4 Excluded by Method 3.81 1 0.0003003 5.405e-05 False High Sequest HT (A2) Unambiguous YAAAAPAATSAASGDAAPAEEAAAEEEEESDDDMGFGLFD [K].yAAAAPAATSAASGDAAPAEEAAAEEEEEsDDDmGFGLFD.[-] N-Term(TMT6plex); S30(Phospho); M34(Oxidation) 1 P05317 P05317 0 4 0.3138 0 1 1 1062.19473 4245.75709 4245.73286 5.71 0.00606 838319.625 CID (Collision Induced Dissociation) MS2 11.04621 60 31.6 60.0 134.9847 46036 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 10.3 26.3 49.2 6.8 21.7 70.5 13.9 25.6 63.7 28.0 Excluded by Method 4.27 1 0.0003003 0.0003212 False High Sequest HT (A2) Unambiguous VVDYSQFQESDDADEDYGRDSGPPTK [K].vVDYSQFQEsDDADEDYGRDSGPPTk.[K] N-Term(TMT6plex); S10(Phospho); K26(TMT6plex) 1 Q9H1E3 Q9H1E3 1 4 0.065 0 1 1 865.38887 3458.53366 3458.53134 0.67 0.00058 398459.125 CID (Collision Induced Dissociation) MS2 11.73811 50 9.8 60.0 134.8887 46003 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 9.2 10.3 9.8 4.0 9.0 12.1 11.4 10.2 13.6 8.0 Excluded by Method 5.08 1 0.0003003 7.836e-06 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVsSDEEF.[-] N-Term(TMT6plex); S8(Phospho) 1 P16521 P16521 0 2 0.0226 0 1 1 885.37065 1769.73402 1769.73562 -0.91 -0.0008 1147990.875 CID (Collision Induced Dissociation) MS2 15.72344 50 99.6 60.0 117.3327 39124 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 22.3 88.0 162.2 28.5 78.2 205.3 90.8 214.6 106.2 Excluded by Method 4.42 1 0.0003003 3.942e-06 False High Sequest HT (A2) Unambiguous CSGPGLSPGMVRANLPQSFQVDTSK [K].cSGPGLsPGMVRANLPQSFQVDTSk.[A] N-Term(TMT6plex); C1(Carbamidomethyl); S7(Phospho); K25(TMT6plex) 2 P21333 P21333; P21333-2 1 4 0.0284 0 1 1 793.64877 3171.57325 3171.57323 0.01 1e-05 2138357.25 CID (Collision Induced Dissociation) MS2 2.614923 20 72.3 37.097 117.231 39085 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 61.0 71.1 68.5 73.9 67.8 83.5 65.7 70.7 89.7 71.3 Excluded by Method 4.22 1 0.0003003 9.531e-05 False High Sequest HT (A2) Unambiguous WYPSEDVAALK [K].wYPsEDVAALk.[K] N-Term(TMT6plex); S4(Phospho); K11(TMT6plex) 1 Q02326 Q02326 0 2 0.274 0 1 1 908.96808 1816.92888 1816.92867 0.11 0.0001 989229.6875 CID (Collision Induced Dissociation) MS2 21.23477 50 54.7 60.0 117.1358 39050 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 25.7 43.4 72.7 31.0 45.9 101.4 20.3 56.8 103.4 45.9 Excluded by Method 3.54 1 0.0003003 9.465e-05 False High Sequest HT (A2) Unambiguous EEEAKEESDDDMGFGLFD [K].eEEAkEEsDDDmGFGLFD.[-] N-Term(TMT6plex); K5(TMT6plex); S8(Phospho); M12(Oxidation) 1 P02400 P02400 1 3 0.6776 0 1 1 873.03681 2617.09587 2617.0941 0.68 0.00059 3367832.0 CID (Collision Induced Dissociation) MS2 0.0 40 210.2 42.302 116.8282 38935 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 52.1 167.0 306.8 69.6 176.1 397.2 80.0 180.1 463.3 209.7 Excluded by Method 3.66 1 0.0003003 0.000468 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVssDEEF.[-] N-Term(TMT6plex); S8(Phospho); S9(Phospho) 1 P16521 P16521 0 2 0.1208 0 1 1 925.35519 1849.70311 1849.70195 0.63 0.00058 3606624.75 CID (Collision Induced Dissociation) MS2 0.0 40 188.4 60.0 116.6951 38884 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 43.5 140.9 312.8 46.9 151.6 368.7 64.6 178.1 390.6 186.7 Excluded by Method 4.8 1 0.0003003 3.038e-05 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVssDEEF.[-] N-Term(TMT6plex); S8(Phospho); S9(Phospho) 1 P16521 P16521 0 2 0.135 0 1 1 925.35489 1849.7025 1849.70195 0.3 0.00027 6356237.0 CID (Collision Induced Dissociation) MS2 0.0 40 178.4 33.175 117.814 39304 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 43.6 144.3 307.5 51.7 139.7 367.5 155.3 379.1 194.8 Excluded by Method 4.37 1 0.0003003 0.0001112 False High Sequest HT (A2) Unambiguous FLNIENPENQSE [K].fLNIENPENQsE.[-] N-Term(TMT6plex); S11(Phospho) 1 Q04779 Q04779 0 2 0.6217 0 1 1 871.89576 1742.78424 1742.78358 0.38 0.00033 3780982.5 CID (Collision Induced Dissociation) MS2 0.0 80 141.4 57.297 118.5424 39575 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 27.2 117.6 243.4 40.8 109.0 280.5 45.9 131.4 272.1 146.0 4.23 1 0.0003003 1.881e-06 False High Sequest HT (A2) Unambiguous TFVLTPELSPGK [K].tFVLTPELsPGk.[L] N-Term(TMT6plex); S9(Phospho); K12(TMT6plex) 1 Q96DY7 Q96DY7 0 3 0.5567 0 1 1 609.67449 1827.00893 1827.00692 1.1 0.00067 771771.0 CID (Collision Induced Dissociation) MS2 17.91509 50 80.8 60.0 118.5097 39563 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 80.2 76.5 63.3 95.4 64.1 69.3 89.3 92.5 92.2 85.0 Excluded by Method 5.64 1 0.0003003 4.101e-05 False High Sequest HT (A2) Unambiguous AYEPDLSDADFEGIEASPK [K].aYEPDLsDADFEGIEASPk.[L] N-Term(TMT6plex); S7(Phospho); K19(TMT6plex) 1 P49687 P49687 0 3 0.1498 0 1 1 864.74349 2592.21591 2592.21587 0.02 2e-05 1786879.125 CID (Collision Induced Dissociation) MS2 0.0 40 54.9 50.852 118.1928 39445 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 15.9 43.1 92.4 18.6 40.1 92.1 22.0 56.7 108.2 59.9 Excluded by Method 4.94 1 0.0003003 6.404e-05 False High Sequest HT (A2) Unambiguous FPEFCSSPSPPVEVK [K].fPEFcSSPsPPVEVk.[I] N-Term(TMT6plex); C5(Carbamidomethyl); S9(Phospho); K15(TMT6plex) 4 Q68E01 Q68E01; Q68E01-4; Q68E01-2; Q68E01-3 0 3 0.1934 0 1 1 749.03935 2245.10351 2245.10163 0.84 0.00063 372688.28125 CID (Collision Induced Dissociation) MS2 23.61819 30 159.4 60.0 118.0129 39379 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 157.5 154.3 136.9 159.0 142.3 146.6 188.3 196.4 152.4 160.3 Excluded by Method 5.48 1 0.0003003 5.326e-07 False High Sequest HT (A2) Unambiguous ESDQTLAALLSPK [K].eSDQTLAALLsPk.[E] N-Term(TMT6plex); S11(Phospho); K13(TMT6plex) 9 P18583 P18583; P18583-4; P18583-6; P18583-5; P18583-10; P18583-9; P18583-2; P18583-3; P18583-7 0 3 0.369 0 1 1 637.67945 1911.02379 1911.02403 -0.13 -8e-05 297507.84375 CID (Collision Induced Dissociation) MS2 7.409939 40 505.2 60.0 118.3345 39497 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 527.9 484.5 399.7 580.8 454.1 475.5 576.2 561.7 511.4 479.8 Excluded by Method 4.39 1 0.0003003 5.947e-05 False High Sequest HT (A2) Unambiguous QGSPDQVSPVSEMTSTSLYQDK [K].qGsPDQVsPVSEMTSTSLYQDk.[Q] N-Term(TMT6plex); S3(Phospho); S8(Phospho); K22(TMT6plex) 1 P46821 P46821 0 3 0.2544 0 1 1 1001.45363 3002.34633 3002.35074 -1.47 -0.00147 457821.625 CID (Collision Induced Dissociation) MS2 0.0 70 20.0 60.0 115.2351 38357 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 21.0 23.3 17.1 18.5 11.8 22.2 25.0 18.2 23.1 20.0 3.38 1 0.0003003 0.0003868 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVSsDEEF.[-] N-Term(TMT6plex); S9(Phospho) 1 P16521 P16521 0 2 0.0111 0 1 1 885.37205 1769.73683 1769.73562 0.68 0.0006 2467538.5 CID (Collision Induced Dissociation) MS2 0.0 50 120.4 60.0 114.9545 38253 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 31.8 101.2 192.6 35.2 94.8 236.5 37.1 113.6 234.4 126.7 Excluded by Method 4.49 1 0.0003003 8.151e-07 False High Sequest HT (A2) Unambiguous AYEPDLSDADFEGIEASPK [K].aYEPDLSDADFEGIEAsPk.[L] N-Term(TMT6plex); S17(Phospho); K19(TMT6plex) 1 P49687 P49687 0 3 0.5342 0 1 1 864.74471 2592.21957 2592.21587 1.43 0.00124 604858.9375 CID (Collision Induced Dissociation) MS2 5.87155 50 42.5 60.0 114.7971 38196 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 12.1 34.0 62.0 10.7 34.2 80.5 17.4 40.5 90.6 42.6 Excluded by Method 5.11 1 0.0003003 2.534e-05 False High Sequest HT (A2) Unambiguous VQGEAVSNIQENTQTPTVQEESEEEEVDETGVEVK [K].vQGEAVSNIQENTQTPTVQEEsEEEEVDETGVEVk.[D] N-Term(TMT6plex); S22(Phospho); K35(TMT6plex) 3 Q13765 Q13765; E9PAV3; E9PAV3-2 0 4 0.0652 0 1 1 1100.51876 4399.0532 4399.06016 -1.58 -0.00174 427125.625 CID (Collision Induced Dissociation) MS2 0.0 50 19.4 60.0 115.3891 38414 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 14.9 20.7 15.4 20.4 16.9 23.9 19.2 22.2 21.5 18.5 Excluded by Method 4.91 1 0.0003003 4.353e-06 False High Sequest HT (A2) Unambiguous EETDTDLEVVLEK [K].eETDtDLEVVLEk.[K] N-Term(TMT6plex); T5(Phospho); K13(TMT6plex) 1 Q1ED39 Q1ED39 0 3 0.0027 0 1 1 686.68137 2058.02956 2058.02957 0.0 0.0 1637852.75 CID (Collision Induced Dissociation) MS2 4.876331 40 110.9 60.0 116.4291 38788 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 113.1 112.9 95.2 108.5 99.6 105.1 120.6 115.2 116.5 122.7 Excluded by Method 3.64 1 0.0003003 0.0001602 False High Sequest HT (A2) Unambiguous LPWEVSDGEVSSGK [K].lPWEVsDGEVSSGk.[T] N-Term(TMT6plex); S6(Phospho); K14(TMT6plex) 1 P48415 P48415 0 3 0.5613 0 1 1 676.67486 2028.01003 2028.00911 0.45 0.00031 3422629.5 CID (Collision Induced Dissociation) MS2 5.555332 70 188.2 31.577 115.6974 38525 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 57.9 163.0 298.9 62.0 161.6 357.9 67.6 187.0 345.1 180.9 4.81 1 0.0003003 9.793e-06 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVssDEEF.[-] N-Term(TMT6plex); S8(Phospho); S9(Phospho) 1 P16521 P16521 0 2 0.1256 0 1 1 925.35446 1849.70165 1849.70195 -0.17 -0.00015 2801820.5 CID (Collision Induced Dissociation) MS2 12.59151 50 159.7 60.0 115.5946 38489 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 38.7 125.9 258.4 40.6 131.2 313.4 57.3 146.3 324.5 161.0 Excluded by Method 4.54 1 0.0003003 3.288e-06 False High Sequest HT (A2) Unambiguous SGLELTELQNMTVPEDDNISNDSNDFTEVENGQINSK [K].sGLELTELQNmTVPEDDNIsNDsNDFTEVENGQINSk.[F] N-Term(TMT6plex); M11(Oxidation); S20(Phospho); S23(Phospho); K37(TMT6plex) 1 Q9H2H9 Q9H2H9 0 4 0.2792 0 1 1 1183.53264 4731.10875 4731.09459 2.99 0.00354 646741.875 CID (Collision Induced Dissociation) MS2 0.0 60 14.8 60.0 118.6762 39625 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 18.3 14.6 11.3 13.6 17.2 10.3 16.8 16.1 15.4 14.4 Excluded by Method 5.91 1 0.0003003 0.0001857 False High Sequest HT (A2) Unambiguous DVLDIEQFSTVK [K].dVLDIEQFsTVk.[G] N-Term(TMT6plex); S9(Phospho); K12(TMT6plex) 4 P34947 P34947; P43250; P43250-2; P43250-3 0 3 0.0028 0 1 1 644.67589 1932.01313 1932.01313 0.0 0.0 929947.8125 CID (Collision Induced Dissociation) MS2 0.0 30 125.0 60.0 122.3211 40973 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 127.5 122.6 101.5 133.8 124.6 106.8 145.2 135.5 124.7 127.4 Excluded by Method 3.53 1 0.0003003 0.0003689 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVssDEEF.[-] N-Term(TMT6plex); S8(Phospho); S9(Phospho) 1 P16521 P16521 0 2 0.1329 0 1 1 925.35513 1849.70299 1849.70195 0.56 0.00052 16123095.0 CID (Collision Induced Dissociation) MS2 0.0 50 162.1 18.684 122.2253 40937 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 40.6 135.1 274.4 40.0 121.9 320.7 52.5 147.8 335.3 153.0 Excluded by Method 4.59 1 0.0003003 4.392e-05 False High Sequest HT (A2) Unambiguous SPQPPVEEEDEHFDDTVVCLDTYNCDLHFK [K].sPQPPVEEEDEHFDDTVVcLDTYNcDLHFk.[I] N-Term(TMT6plex); S1(Phospho); C19(Carbamidomethyl); C25(Carbamidomethyl); K30(TMT6plex) 2 Q00839 Q00839-2; Q00839 0 4 0.4669 0 1 1 1044.21808 4173.85048 4173.84991 0.14 0.00014 411335.40625 CID (Collision Induced Dissociation) MS2 9.290144 70 12.0 60.0 122.323 40974 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 7.5 17.4 9.9 12.9 9.0 15.3 13.0 12.8 10.5 11.4 8.91 1 0.0003003 1.321e-07 False High Sequest HT (A2) Unambiguous IVVVRAEELNISGEFFRNK [K].iVVVRAEELNISGEFFRNk.[L] N-Term(TMT6plex); K19(TMT6plex) 1 P26784 P26784 2 4 0.5073 0 1 1 670.39063 2678.54069 2678.53939 0.49 0.00033 1677860.875 CID (Collision Induced Dissociation) MS2 8.332108 40 64.7 55.939 121.2226 40568 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 14.9 43.5 102.6 17.1 57.0 134.9 22.0 66.7 130.6 57.9 Excluded by Method 5.5 1 0.0003003 1.454e-06 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVssDEEF.[-] N-Term(TMT6plex); S8(Phospho); S9(Phospho) 1 P16521 P16521 0 2 0.1279 0 1 1 925.35501 1849.70275 1849.70195 0.43 0.0004 15918989.0 CID (Collision Induced Dissociation) MS2 1.926869 40 211.2 14.417 123.353 41343 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 54.9 175.0 365.3 58.1 166.5 453.3 195.1 434.2 210.0 Excluded by Method 4.69 1 0.0003003 8.526e-05 False High Sequest HT (A2) Unambiguous LFQLGPPSPVK [K].lFQLGPPsPVk.[M] N-Term(TMT6plex); S8(Phospho); K11(TMT6plex) 3 O95997 O95997; Q9NZH5; Q9NZH5-2 0 3 0.4502 0 1 1 574.33197 1720.98135 1720.98032 0.6 0.00034 1701927.625 CID (Collision Induced Dissociation) MS2 4.158803 50 245.1 60.0 122.9562 41202 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 268.6 267.8 207.3 249.2 215.8 228.1 285.6 249.2 249.6 229.3 Excluded by Method 3.11 1 0.0003003 2.978e-05 False High Sequest HT (A2) Unambiguous VPTVGSYGVAGATLPETIPTSK [K].vPTVGsYGVAGATLPETIPTSk.[N] N-Term(TMT6plex); S6(Phospho); K22(TMT6plex) 1 P38250 P38250 0 3 0.0084 0 1 1 895.15054 2683.43706 2683.43597 0.41 0.00036 1569449.375 CID (Collision Induced Dissociation) MS2 0.0 30 208.5 60.0 122.942 41196 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 53.9 167.0 343.3 60.8 163.9 402.1 68.6 204.4 413.3 208.0 Excluded by Method 4.75 1 0.0003003 9.23e-06 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVssDEEF.[-] N-Term(TMT6plex); S8(Phospho); S9(Phospho) 1 P16521 P16521 0 2 0.1503 0 1 1 925.35465 1849.70201 1849.70195 0.03 3e-05 13234061.0 CID (Collision Induced Dissociation) MS2 0.7339803 40 200.4 19.542 121.1301 40534 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 51.8 167.7 338.5 62.0 160.6 416.5 180.4 409.1 217.2 Excluded by Method 4.79 1 0.0003003 2.518e-05 False High Sequest HT (A2) Unambiguous EETPPPVEPEEEEDTEDAGLDDWEAMASDEETEK [K].eETPPPVEPEEEEDTEDAGLDDWEAMAsDEETEk.[V] N-Term(TMT6plex); S28(Phospho); K34(TMT6plex) 1 O60841 O60841 0 4 0.1006 0 1 1 1097.4653 4386.83935 4386.84203 -0.61 -0.00067 237223.109375 CID (Collision Induced Dissociation) MS2 0.0 60 28.2 60.0 121.0887 40519 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 29.9 25.0 22.5 25.1 22.9 34.5 34.7 34.1 28.9 24.6 Excluded by Method 4.87 1 0.0003003 0.0005603 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVssDEEF.[-] N-Term(TMT6plex); S8(Phospho); S9(Phospho) 1 P16521 P16521 0 2 0.1213 0 1 1 925.35495 1849.70262 1849.70195 0.36 0.00034 11239312.0 CID (Collision Induced Dissociation) MS2 1.14129 40 195.8 18.231 119.9923 40111 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 44.8 159.5 322.6 52.8 148.2 414.5 185.5 419.7 210.6 Excluded by Method 4.7 1 0.0003003 1.822e-05 False High Sequest HT (A2) Unambiguous NIEDVIAQGIGK [K].nIEDVIAQGIGk.[L] N-Term(TMT6plex); K12(TMT6plex) 1 P05387 P05387 0 3 0.485 0 1 1 572.34174 1715.01066 1715.01036 0.17 0.0001 1670025.625 CID (Collision Induced Dissociation) MS2 0.0 50 267.7 60.0 118.9781 39737 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 288.2 283.5 236.5 258.1 243.7 260.5 311.9 278.9 269.2 246.0 Excluded by Method 3.67 1 0.0003003 0.0001334 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVssDEEF.[-] N-Term(TMT6plex); S8(Phospho); S9(Phospho) 1 P16521 P16521 0 2 0.1293 0 1 1 925.35507 1849.70287 1849.70195 0.49 0.00046 7957197.0 CID (Collision Induced Dissociation) MS2 5.772198 40 169.8 34.294 118.9063 39711 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 41.3 134.3 285.5 46.3 146.6 339.2 171.2 356.3 177.5 Excluded by Method 4.64 1 0.0003003 4.198e-06 False High Sequest HT (A2) Unambiguous GVNLPGTDVDLPALSEK [K].gVNLPGTDVDLPALSEk.[D] N-Term(TMT6plex); K17(TMT6plex) 1 P00549 P00549 0 3 0.5 0 1 1 728.41616 2183.23393 2183.23237 0.71 0.00052 629992.375 CID (Collision Induced Dissociation) MS2 22.40914 80 66.0 60.0 120.6606 40362 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 15.5 55.2 90.8 18.5 51.7 141.0 24.8 68.1 125.3 68.8 4.7 1 0.0003003 3.637e-05 False High Sequest HT (A2) Unambiguous RVTFGEDLSPEVFDESLPANTPLRK [K].rVTFGEDLsPEVFDESLPANTPLRk.[G] N-Term(TMT6plex); S9(Phospho); K25(TMT6plex) 1 Q69YH5 Q69YH5 2 4 0.1921 0 1 1 839.68854 3355.73234 3355.73394 -0.48 -0.0004 789620.0 CID (Collision Induced Dissociation) MS2 25.29743 40 41.1 60.0 120.6312 40351 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 51.3 39.8 33.8 41.3 40.6 42.9 42.0 46.2 42.2 31.3 Excluded by Method 4.84 1 0.0003003 0.0001087 False High Sequest HT (A2) Unambiguous RAVDNIPVGPNFDDEEDEGEDLCNCEFSLAYGAK [K].rAVDNIPVGPNFDDEEDEGEDLcNcEFSLAYGAk.[I] N-Term(TMT6plex); C23(Carbamidomethyl); C25(Carbamidomethyl); K34(TMT6plex) 1 P16521 P16521 1 4 0.5548 0 1 1 1076.75061 4303.98062 4303.98 0.14 0.00015 582884.4375 CID (Collision Induced Dissociation) MS2 0.0 60 29.4 60.0 120.42 40274 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 3.6 26.0 51.6 7.0 27.6 51.7 10.3 25.7 57.8 32.6 Excluded by Method 5.84 1 0.0003003 4.633e-06 False High Sequest HT (A2) Unambiguous SPSFASEWDEIEK [K].sPsFASEWDEIEk.[I] N-Term(TMT6plex); S3(Phospho); K13(TMT6plex) 1 Q92625 Q92625 0 3 0.002 0 1 1 688.33072 2062.97761 2062.97747 0.07 5e-05 1698806.75 CID (Collision Induced Dissociation) MS2 4.799522 30 143.9 53.334 120.1941 40186 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 149.5 147.2 120.5 153.7 129.3 138.7 162.5 151.0 147.7 139.1 Excluded by Method 5.0 1 0.0003003 5.661e-05 False High Sequest HT (A2) Unambiguous LALSPLSLGK [K].lALsPLSLGk.[E] N-Term(TMT6plex); S4(Phospho); K10(TMT6plex) 1 P49454 P49454 0 2 0.1535 0 1 1 768.96208 1536.91688 1536.91665 0.15 0.00011 1608459.875 CID (Collision Induced Dissociation) MS2 32.3145 40 131.7 49.036 124.1229 41708 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 139.6 136.5 117.6 127.4 118.4 143.1 141.8 141.0 120.5 131.4 Excluded by Method 4.04 1 0.0003003 0.0002077 False High Sequest HT (A2) Unambiguous DVNPWLANESDEEHTVK [K].dVNPWLANEsDEEHTVk.[K] N-Term(TMT6plex); S10(Phospho); K17(TMT6plex) 1 Q04500 Q04500 0 3 0.1101 0 1 1 841.07239 2521.20261 2521.20122 0.55 0.00046 4769826.5 CID (Collision Induced Dissociation) MS2 5.403852 80 59.6 24.57 94.0601 30438 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 28.4 49.7 86.7 29.9 48.0 98.6 29.7 57.0 104.4 63.4 5.63 1 0.0003003 3.146e-06 False High Sequest HT (A2) Unambiguous VVDYSQFQESDDADEDYGRDSGPPTK [K].vVDYSQFQEsDDADEDYGRDSGPPTk.[K] N-Term(TMT6plex); S10(Phospho); K26(TMT6plex) 1 Q9H1E3 Q9H1E3 1 4 0.0785 0 1 1 865.38912 3458.53464 3458.53134 0.95 0.00083 464794.03125 CID (Collision Induced Dissociation) MS2 18.23711 60 29.8 54.984 93.8774 30374 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 29.3 32.3 29.2 26.4 28.2 28.7 29.8 27.7 34.6 31.4 Excluded by Method 7.13 1 0.0003003 2.481e-07 False High Sequest HT (A2) Unambiguous AEASGEAAEEAEDEE [K].aEAsGEAAEEAEDEE.[-] N-Term(TMT6plex); S4(Phospho) 1 P05755 P05755 0 2 0.8281 0 1 1 923.35929 1845.7113 1845.71126 0.02 2e-05 1182533.5 CID (Collision Induced Dissociation) MS2 22.1325 90 43.2 60.0 54.6077 15660 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 10.9 34.4 72.7 12.7 32.6 85.7 11.7 35.2 96.0 40.5 4.13 1 0.0003003 8.928e-05 False High Sequest HT (A2) Unambiguous EEDEEPESPPEK [K].eEDEEPEsPPEk.[K] N-Term(TMT6plex); S8(Phospho); K12(TMT6plex) 2 Q9H1E3 Q9H1E3; Q9H1E3-2 0 2 0.5229 0 1 1 976.94214 1952.877 1952.87782 -0.42 -0.00041 639015.5625 CID (Collision Induced Dissociation) MS2 0.0 40 171.3 60.0 54.4525 15603 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 179.8 164.4 130.6 191.8 155.8 154.3 198.3 185.0 175.2 177.9 Excluded by Method 4.15 1 0.0003003 3.896e-05 False High Sequest HT (A2) Unambiguous EPEGPPPSPGK [K].ePEGPPPsPGk.[G] N-Term(TMT6plex); S8(Phospho); K11(TMT6plex) 1 P58012 P58012 0 3 0.6414 0 1 1 543.94793 1629.82923 1629.82896 0.17 9e-05 837059.1875 CID (Collision Induced Dissociation) MS2 0.0 70 194.5 60.0 53.9095 15404 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 206.6 202.7 152.8 220.9 181.3 182.7 222.8 193.3 193.2 188.3 5.27 1 0.0003003 5.188e-06 False High Sequest HT (A2) Unambiguous RAASEESDVEEDEDK [K].rAAsEEsDVEEDEDk.[L] N-Term(TMT6plex); S4(Phospho); S7(Phospho); K15(TMT6plex) 1 Q04660 Q04660 1 3 0.5532 0 1 1 776.328 2326.96945 2326.97293 -1.5 -0.00116 264158.96875 CID (Collision Induced Dissociation) MS2 0.0 50 132.6 60.0 53.5721 15280 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 35.7 109.1 184.3 45.4 116.6 241.6 50.4 126.0 286.9 130.3 Excluded by Method 6.11 1 0.0003003 4.079e-05 False High Sequest HT (A2) Unambiguous AEDGATPSPSNETPK [K].aEDGATPsPSNETPk.[K] N-Term(TMT6plex); S8(Phospho); K15(TMT6plex) 1 P29966 P29966 0 2 0.0746 0 1 1 1019.99101 2038.97474 2038.97345 0.63 0.00065 2486040.5 CID (Collision Induced Dissociation) MS2 0.0 60 132.2 60.0 53.493 15250 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 136.8 127.9 103.6 138.1 126.9 133.2 152.0 132.4 133.4 137.3 Excluded by Method 4.69 1 0.0003003 1.064e-06 False High Sequest HT (A2) Unambiguous AEASGEAAEEAEDEE [K].aEASGEAAEEAEDEE.[-] N-Term(TMT6plex) 1 P05755 P05755 0 2 0.7333 0 1 1 883.377 1765.74672 1765.74493 1.02 0.0009 135212.0625 CID (Collision Induced Dissociation) MS2 0.0 100 15.3 60.0 54.2028 15511 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 3.0 17.7 23.3 1.9 10.4 27.7 8.4 15.5 29.0 15.6 2.55 1 0.0003003 0.0001756 False High Sequest HT (A2) Unambiguous HIQEEEEQDVDMTGDEEQEEEPDREK [K].hIQEEEEQDVDmtGDEEQEEEPDREk.[I] N-Term(TMT6plex); M12(Oxidation); T13(Phospho); K26(TMT6plex) 1 P28000 P28000 1 4 0.4312 0 1 1 932.65065 3727.58078 3727.58424 -0.93 -0.00086 445581.5625 CID (Collision Induced Dissociation) MS2 0.0 60 5.0 60.0 55.4579 15982 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 2.4 10.4 1.7 2.5 10.5 2.6 2.1 14.5 3.4 Excluded by Method 4.87 1 0.0003003 0.0001492 False High Sequest HT (A2) Unambiguous ADESDPVESK [K].aDEsDPVESk.[K] N-Term(TMT6plex); S4(Phospho); K10(TMT6plex) 1 P38174 P38174 0 2 0.3709 0 1 1 807.88824 1614.76921 1614.76642 1.73 0.0014 846998.375 CID (Collision Induced Dissociation) MS2 17.86557 40 243.3 60.0 55.497 15998 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 64.9 198.6 358.1 80.4 198.6 450.3 83.9 235.8 516.9 245.7 Excluded by Method 4.26 1 0.0003003 0.000389 False High Sequest HT (A2) Unambiguous QFEDHQSSEEDASEK [K].qFEDHQssEEDASEk.[D] N-Term(TMT6plex); S7(Phospho); S8(Phospho); K15(TMT6plex) 1 P40477 P40477 0 3 0.6234 0 1 1 795.32839 2383.97062 2383.97327 -1.11 -0.00088 268671.59375 CID (Collision Induced Dissociation) MS2 47.62836 70 30.7 60.0 57.1772 16622 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 10.7 27.0 41.7 16.4 29.3 59.3 15.7 28.2 54.4 24.2 3.93 1 0.0003003 0.0003457 False High Sequest HT (A2) Unambiguous SNNEVTEHSDSEDLTEK [K].sNNEVTEHsDSEDLTEk.[Q] N-Term(TMT6plex); S9(Phospho); K17(TMT6plex) 1 P40563 P40563 0 3 0.171 0 1 1 824.71109 2472.11872 2472.11794 0.31 0.00026 3188317.75 CID (Collision Induced Dissociation) MS2 2.388749 60 40.2 49.658 57.0479 16573 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 9.5 36.1 52.2 10.2 36.4 76.3 14.3 41.3 85.9 39.7 Excluded by Method 4.97 1 0.0003003 3.824e-05 False High Sequest HT (A2) Unambiguous RESVASGDDRAEEDMDEAIEK [K].rEsVASGDDRAEEDmDEAIEk.[G] N-Term(TMT6plex); S3(Phospho); M15(Oxidation); K21(TMT6plex) 1 P46821 P46821 2 4 0.1342 0 1 1 727.33401 2906.31421 2906.3127 0.52 0.00038 671864.3125 CID (Collision Induced Dissociation) MS2 5.736363 50 50.7 60.0 56.8026 16484 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 41.5 52.0 40.2 55.1 40.8 51.4 62.3 55.4 55.6 52.7 Excluded by Method 5.59 1 0.0003003 8.973e-06 False High Sequest HT (A2) Unambiguous TAQSNGNDDEDASPQLK [K].tAQSNGNDDEDAsPQLk.[L] N-Term(TMT6plex); S13(Phospho); K17(TMT6plex) 1 Q04500 Q04500 0 3 0.4294 0 1 1 776.69738 2328.0776 2328.07568 0.82 0.00064 594313.375 CID (Collision Induced Dissociation) MS2 26.13707 40 18.5 60.0 56.7367 16460 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 10.3 15.6 26.2 12.1 9.1 26.0 11.7 16.5 38.8 18.6 Excluded by Method 3.61 1 0.0003003 4.702e-05 False High Sequest HT (A2) Unambiguous STSAPQMSPGSSDNQSSSPQPAQQK [K].sTSAPQMsPGSSDNQSSSPQPAQQk.[L] N-Term(TMT6plex); S8(Phospho); K25(TMT6plex) 5 Q14157-1 Q14157-1; Q14157-5; Q14157-4; Q14157; Q14157-3 0 3 0.0286 0 1 1 1024.14512 3070.42079 3070.41889 0.62 0.00063 361228.78125 CID (Collision Induced Dissociation) MS2 0.0 70 35.5 60.0 56.3972 16335 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 31.9 38.1 27.7 30.5 31.3 37.1 42.7 40.0 38.9 37.3 5.94 1 0.0003003 9.839e-06 False High Sequest HT (A2) Unambiguous VNGDASPAAAESGAK [K].vNGDAsPAAAESGAk.[E] N-Term(TMT6plex); S6(Phospho); K15(TMT6plex) 1 P29966 P29966 0 3 0.3349 0 1 1 628.31563 1882.93235 1882.93119 0.61 0.00039 11075213.0 CID (Collision Induced Dissociation) MS2 4.930374 60 74.2 18.78 56.6968 16445 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 71.3 78.9 63.9 70.0 69.5 79.9 65.9 88.0 70.4 84.2 Excluded by Method 4.15 1 0.0003003 6.562e-06 False High Sequest HT (A2) Unambiguous NSSQQDNSSRESPSLEDEETK [K].nSSQQDNSSREsPSLEDEETk.[K] N-Term(TMT6plex); S12(Phospho); K21(TMT6plex) 2 Q96T23 Q96T23; Q96T23-2 1 3 0.1038 0 1 1 969.10945 2905.31379 2905.31005 1.29 0.00125 361249.90625 CID (Collision Induced Dissociation) MS2 0.0 40 53.7 60.0 50.9312 14301 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 45.7 53.0 44.0 60.6 49.9 50.9 61.7 57.9 51.1 62.1 Excluded by Method 3.95 1 0.0003003 0.0003459 False High Sequest HT (A2) Unambiguous AEDGATPSPSNETPK [K].aEDGATPSPSNEtPk.[K] N-Term(TMT6plex); T13(Phospho); K15(TMT6plex) 1 P29966 P29966 0 2 0.0909 0 1 1 1019.99009 2038.97291 2038.97345 -0.27 -0.00027 254263.859375 CID (Collision Induced Dissociation) MS2 40.17272 50 114.6 60.0 49.9931 13959 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 113.6 97.0 87.6 134.3 106.7 119.2 134.8 111.8 119.3 121.4 Excluded by Method 5.39 1 0.0003003 3.634e-09 False High Sequest HT (A2) Unambiguous NEATPEAEQVK [K].nEAtPEAEQVk.[K] N-Term(TMT6plex); T4(Phospho); K11(TMT6plex) 1 Q08245 Q08245 0 2 0.3719 0 1 1 877.4425 1753.87773 1753.87737 0.2 0.00018 19634506.0 CID (Collision Induced Dissociation) MS2 6.419937 40 279.4 14.438 53.178 15133 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 55.2 226.6 437.4 91.3 233.9 545.4 92.3 247.4 583.3 281.1 Excluded by Method 4.06 1 0.0003003 0.0002104 False High Sequest HT (A2) Unambiguous GDDDEESDEEAVK [K].gDDDEEsDEEAVk.[K] N-Term(TMT6plex); S7(Phospho); K13(TMT6plex) 1 O43395 O43395 0 2 0.5243 0 1 1 988.4257 1975.84412 1975.84216 0.99 0.00098 249201.75 CID (Collision Induced Dissociation) MS2 0.0 30 221.1 60.0 52.9249 15040 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 204.7 220.7 167.2 249.7 215.4 207.5 261.5 247.3 215.8 221.0 Excluded by Method 5.76 1 0.0003003 1.386e-08 False High Sequest HT (A2) Unambiguous SQENLGNPSK [K].sQENLGNPSk.[N] N-Term(TMT6plex); S1(Phospho); K10(TMT6plex) 2 Q9H0X4 Q9H0X4-2; Q9H0X4 0 2 0.63 0 1 1 806.41071 1611.81414 1611.81437 -0.14 -0.00012 1173711.5 CID (Collision Induced Dissociation) MS2 0.0 40 58.8 60.0 52.8492 15011 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 63.2 68.2 47.1 59.4 54.8 53.7 64.5 57.5 50.6 69.1 Excluded by Method 3.73 1 0.0003003 0.0001659 False High Sequest HT (A2) Unambiguous SPPAEEAASPEK [K].sPPAEEAAsPEk.[E] N-Term(TMT6plex); S9(Phospho); K12(TMT6plex) 2 P12036 P12036; P12036-2 0 3 0.5037 0 1 1 584.29392 1750.86722 1750.86647 0.43 0.00025 1495818.75 CID (Collision Induced Dissociation) MS2 4.634153 70 127.3 60.0 52.5287 14894 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 130.8 121.8 99.8 155.7 122.3 127.7 143.1 127.9 124.3 119.9 5.42 1 0.0003003 2.087e-06 False High Sequest HT (A2) Unambiguous SDQQGEALDSSQK [K].sDQQGEALDSSQk.[K] N-Term(TMT6plex); S1(Phospho); K13(TMT6plex) 3 Q32MZ4 Q32MZ4-2; Q32MZ4; Q32MZ4-3 0 3 0.4773 0 1 1 644.31133 1930.91944 1930.91594 1.82 0.00117 507202.84375 CID (Collision Induced Dissociation) MS2 36.14243 60 26.5 60.0 52.3132 14814 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 24.7 22.6 18.5 28.2 26.6 29.0 29.3 30.8 29.0 26.1 Excluded by Method 3.08 1 0.0003003 0.0003336 False High Sequest HT (A2) Unambiguous AEASGEAAEEAEDEE [K].aEASGEAAEEAEDEE.[-] N-Term(TMT6plex) 1 P05755 P05755 0 2 0.8374 0 1 1 883.37578 1765.74428 1765.74493 -0.37 -0.00032 443999.34375 CID (Collision Induced Dissociation) MS2 0.0 100 281.7 60.0 51.9829 14692 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 64.2 232.6 439.0 75.2 230.0 559.6 97.2 253.4 576.7 288.7 5.29 1 0.0003003 2.251e-09 False High Sequest HT (A2) Unambiguous GEPAAAAAPEAGASPVEK [K].gEPAAAAAPEAGAsPVEk.[E] N-Term(TMT6plex); S14(Phospho); K18(TMT6plex) 1 P29966 P29966 0 3 0.5918 0 1 1 721.03623 2161.09414 2161.09424 -0.05 -3e-05 2654905.75 CID (Collision Induced Dissociation) MS2 11.79279 70 162.4 60.0 66.6556 20153 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 168.0 166.0 125.9 160.7 163.4 151.0 190.3 165.6 165.3 168.2 6.37 1 0.0003003 1.923e-09 False High Sequest HT (A2) Unambiguous YRIQEQESSGEEDSDLSPEEREK [K].yRIQEQESsGEEDSDLSPEEREk.[K] N-Term(TMT6plex); S9(Phospho); K23(TMT6plex) 2 Q6NXS1 Q6NXS1; P41236 2 4 0.0462 0 1 1 820.38297 3278.51006 3278.51021 -0.04 -4e-05 727762.4375 CID (Collision Induced Dissociation) MS2 12.27549 30 18.2 59.336 65.8503 19858 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 15.5 17.8 13.4 19.0 20.1 16.4 20.6 16.8 20.6 22.2 Excluded by Method 4.11 1 0.0003003 6.279e-05 False High Sequest HT (A2) Unambiguous NIGRDTPTSAGPNSFNK [K].nIGRDtPTSAGPNSFNk.[G] N-Term(TMT6plex); T6(Phospho); K17(TMT6plex) 2 Q8WW12 Q8WW12; Q8WW12-3 1 3 0.062 0 1 1 772.05787 2314.15904 2314.1593 -0.11 -8e-05 711809.375 CID (Collision Induced Dissociation) MS2 49.38659 50 107.9 60.0 67.7033 20551 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 110.7 98.3 85.9 111.2 106.2 116.3 118.4 116.7 108.6 106.9 Excluded by Method 5.0 1 0.0003003 2.986e-06 False High Sequest HT (A2) Unambiguous AGLDNVDAESK [K].aGLDNVDAEsk.[E] N-Term(TMT6plex); S10(Phospho); K11(TMT6plex) 1 P09064 P09064 0 3 0.4787 0 1 1 552.9462 1656.82405 1656.8246 -0.33 -0.00018 1187862.875 CID (Collision Induced Dissociation) MS2 34.385 90 198.9 56.098 67.5739 20502 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 45.5 164.4 306.0 68.7 169.6 371.2 66.3 188.5 409.3 199.5 4.7 1 0.0003003 0.0001019 False High Sequest HT (A2) Unambiguous EQLSETEGSGEDEPGNDPSETTQK [K].eQLsETEGsGEDEPGNDPSETTQk.[K] N-Term(TMT6plex); S4(Phospho); S9(Phospho); K24(TMT6plex) 2 Q13107 Q13107-2; Q13107 0 3 0.652 0 1 1 1061.449 3182.33246 3182.33396 -0.47 -0.0005 2366703.5 CID (Collision Induced Dissociation) MS2 0.0 50 40.2 60.0 67.5358 20488 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 36.7 40.4 31.8 44.9 28.8 42.3 49.8 49.1 36.3 41.7 Excluded by Method 7.04 1 0.0003003 1.808e-06 False High Sequest HT (A2) Unambiguous EVSEEENTEPEVQEEK [K].eVsEEENtEPEVQEEk.[E] N-Term(TMT6plex); S3(Phospho); T8(Phospho); K16(TMT6plex) 1 P38249 P38249 0 3 0.593 0 1 1 841.70081 2523.08786 2523.08288 1.98 0.00166 317306.09375 CID (Collision Induced Dissociation) MS2 0.0 40 50.3 60.0 67.5055 20477 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 13.6 38.0 66.5 12.5 43.5 96.9 18.0 47.9 111.6 54.6 Excluded by Method 5.16 1 0.0003003 5.582e-05 False High Sequest HT (A2) Unambiguous EVSEEENTEPEVQEEK [K].eVsEEENTEPEVQEEk.[E] N-Term(TMT6plex); S3(Phospho); K16(TMT6plex) 1 P38249 P38249 0 3 0.0327 0 1 1 815.04308 2443.11468 2443.11655 -0.76 -0.00062 3182594.25 CID (Collision Induced Dissociation) MS2 10.42732 40 74.3 48.38 66.7992 20209 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 19.5 60.1 103.8 23.1 59.2 136.5 26.4 73.9 150.7 89.9 Excluded by Method 3.98 1 0.0003003 0.0003872 False High Sequest HT (A2) Unambiguous NGVAAEVSPAK [K].nGVAAEVsPAk.[E] N-Term(TMT6plex); S8(Phospho); K11(TMT6plex) 1 Q9BW71 Q9BW71 0 3 0.4459 0 1 1 527.61971 1580.84458 1580.84494 -0.23 -0.00012 7623977.0 CID (Collision Induced Dissociation) MS2 7.380174 80 130.5 44.125 60.1531 17723 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 97.6 125.4 129.4 126.1 117.3 163.5 122.7 129.8 163.0 130.2 5.36 1 0.0003003 5.362e-05 False High Sequest HT (A2) Unambiguous SSETSPDSTPAPSAPASTNAPTNNK [K].sSETsPDSTPAPSAPASTNAPTNNk.[E] N-Term(TMT6plex); S5(Phospho); K25(TMT6plex) 1 P53235 P53235 0 3 0.0205 0 1 1 989.80466 2967.39944 2967.39848 0.32 0.00032 957826.5625 CID (Collision Induced Dissociation) MS2 0.0 50 42.3 60.0 58.3546 17054 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 12.3 29.6 72.8 11.2 30.4 84.2 12.6 42.1 86.1 41.8 Excluded by Method 4.88 1 0.0003003 3.693e-05 False High Sequest HT (A2) Unambiguous RKPSPEPEGEVGPPK [K].rkPsPEPEGEVGPPk.[I] N-Term(TMT6plex); K2(TMT6plex); S4(Phospho); K15(TMT6plex) 2 Q7Z5L9 Q7Z5L9-2; Q7Z5L9 1 4 0.5588 0 1 1 593.58053 2371.30028 2371.29897 0.55 0.00033 1275042.875 CID (Collision Induced Dissociation) MS2 6.470994 40 205.6 58.635 57.9197 16896 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 233.1 200.5 167.3 223.6 200.0 186.6 244.6 206.4 185.7 208.3 Excluded by Method 4.08 1 0.0003003 6.013e-05 False High Sequest HT (A2) Unambiguous NEPEDEEEEEEEEDEDEEEEDEDEE [K].nEPEDEEEEEEEEDEDEEEEDEDEE.[-] N-Term(TMT6plex) 1 P26583 P26583 0 3 0.9497 0 1 1 1119.73112 3357.17882 3357.17785 0.29 0.00032 1563006.75 CID (Collision Induced Dissociation) MS2 0.0 80 25.5 60.0 60.7371 17943 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 27.4 25.4 21.0 28.4 26.2 27.0 25.9 24.7 24.3 24.2 7.95 1 0.0003003 8.654e-09 False High Sequest HT (A2) Unambiguous LVEQANSPK [K].lVEQANsPk.[H] N-Term(TMT6plex); S7(Phospho); K9(TMT6plex) 1 O95391 O95391 0 3 0.4027 0 1 1 508.61218 1523.82198 1523.82348 -0.99 -0.0005 1122836.25 CID (Collision Induced Dissociation) MS2 0.0 90 49.1 60.0 63.1679 18862 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 48.0 47.8 36.5 46.6 47.9 41.2 58.6 55.3 50.3 59.2 4.47 1 0.0003003 0.0001151 False High Sequest HT (A2) Unambiguous ATVTPSPVK [K].aTVTPsPVk.[G] N-Term(TMT6plex); S6(Phospho); K9(TMT6plex) 2 Q9H1E3 Q9H1E3; Q9H1E3-2 0 2 0.2254 0 1 1 719.40958 1437.81189 1437.81185 0.03 2e-05 56248380.0 CID (Collision Induced Dissociation) MS2 1.680085 50 261.9 4.77 63.0535 18817 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 256.7 258.7 215.0 285.4 258.7 247.9 307.2 295.1 238.7 255.9 Excluded by Method 4.17 1 0.0003003 0.0004235 False High Sequest HT (A2) Unambiguous ELDQDMVTEDEDDPGSHK [K].eLDQDmVtEDEDDPGSHk.[R] N-Term(TMT6plex); M6(Oxidation); T8(Phospho); K18(TMT6plex) 2 Q9NRL2 Q9NRL2; Q9NRL2-2 0 3 0.4461 0 1 1 872.04706 2614.12664 2614.12679 -0.06 -5e-05 1668819.25 CID (Collision Induced Dissociation) MS2 0.0 20 73.0 60.0 62.3432 18548 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 84.1 70.5 66.0 57.6 70.5 74.8 81.2 80.6 61.8 82.8 Excluded by Method 4.73 1 0.0003003 1.122e-05 False High Sequest HT (A2) Unambiguous QFANSNNNNNDSGNNNQGDYVTK [K].qFANSNNNNNDSGNNNQGDYVTk.[A] N-Term(TMT6plex); K23(TMT6plex) 1 P18899 P18899 0 4 0.4419 0 1 1 747.60247 2987.38803 2987.38799 0.02 1e-05 2131543.25 CID (Collision Induced Dissociation) MS2 39.20634 50 55.0 39.527 62.0056 18420 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 15.8 44.8 73.1 21.5 45.2 107.3 18.2 47.3 119.3 57.3 Excluded by Method 3.87 1 0.0003003 0.0004087 False High Sequest HT (A2) Unambiguous AEGEPQEESPLK [K].aEGEPQEEsPLk.[S] N-Term(TMT6plex); S9(Phospho); K12(TMT6plex) 4 Q9NYF8 Q9NYF8-3; Q9NYF8-4; Q9NYF8; Q9NYF8-2 0 2 0.5952 0 1 1 926.46066 1851.91405 1851.91415 -0.05 -5e-05 1368685.5 CID (Collision Induced Dissociation) MS2 0.0 50 149.0 60.0 61.4806 18224 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 147.8 149.5 115.2 158.9 141.1 143.6 173.5 156.0 150.7 153.6 Excluded by Method 4.99 1 0.0003003 1.514e-05 False High Sequest HT (A2) Unambiguous EDQEEALSDNEPESSEK [K].eDQEEALsDNEPESSEk.[F] N-Term(TMT6plex); S8(Phospho); K17(TMT6plex) 1 P34250 P34250 0 3 0.4431 0 1 1 825.3674 2474.08765 2474.08597 0.68 0.00056 1123422.0 CID (Collision Induced Dissociation) MS2 0.0 40 43.9 60.0 61.3598 18180 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 11.0 31.8 67.7 17.8 35.9 85.2 16.3 39.8 89.4 44.2 Excluded by Method 4.04 1 0.0003003 0.0003836 False High Sequest HT (A2) Unambiguous GTSQVGELSQSSTK [K].gTSQVGELSQsSTk.[L] N-Term(TMT6plex); S11(Phospho); K14(TMT6plex) 1 P32604 P32604 0 3 0.0488 0 1 1 649.66679 1946.98582 1946.98362 1.13 0.00073 2140286.25 CID (Collision Induced Dissociation) MS2 51.27145 90 121.1 60.0 61.3464 18175 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 31.4 99.7 186.6 32.9 100.4 243.6 44.5 107.3 239.7 124.6 4.3 1 0.0003003 2.272e-05 False High Sequest HT (A2) Unambiguous DTSATSQSVNGSPQAEQPSLESTSK [K].dTSATSQSVNGsPQAEQPSLESTSk.[E] N-Term(TMT6plex); S12(Phospho); K25(TMT6plex) 3 Q86WB0 Q86WB0-2; Q86WB0-3; Q86WB0 0 3 0.1689 0 1 1 1025.49069 3074.45753 3074.45672 0.26 0.00027 331565.3125 CID (Collision Induced Dissociation) MS2 0.0 60 72.5 60.0 67.721 20558 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 78.7 78.1 57.1 79.0 72.4 68.4 78.2 76.3 67.7 69.0 Excluded by Method 7.34 1 0.0003003 3.952e-07 False High Sequest HT (A2) Unambiguous GRLVREDENDASDDEDDDEK [K].gRLVREDENDAsDDEDDDEk.[R] N-Term(TMT6plex); S12(Phospho); K20(TMT6plex) 4 Q9Y5B6 Q9Y5B6-4; Q9Y5B6-3; Q9Y5B6; Q9Y5B6-2 2 3 0.5383 0 1 1 954.08946 2860.25382 2860.2522 0.56 0.00054 1296267.125 CID (Collision Induced Dissociation) MS2 0.0 10 20.1 60.0 48.4485 13390 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 21.6 21.2 13.3 19.4 19.6 18.3 21.8 21.3 25.4 19.5 Excluded by Method 4.7 1 0.0003003 4.341e-07 False High Sequest HT (A2) Unambiguous EEEAAEESDDDMGFGLFD [K].eEEAAEEsDDDMGFGLFD.[-] N-Term(TMT6plex); S8(Phospho) 2 P05319 P10622; P05319 0 3 0.8504 0 1 1 772.29822 2314.88011 2314.8784 0.74 0.00057 437105.375 CID (Collision Induced Dissociation) MS2 9.409959 80 31.1 60.0 137.9169 47041 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 10.0 27.8 51.7 4.0 21.9 62.7 12.8 25.3 59.8 35.2 4.68 1 0.0003003 1.511e-05 False High Sequest HT (A2) Unambiguous YAAAAPAATSAASGDAAPAEEAAAEEEEESDDDMGFGLFD [K].yAAAAPAATSAASGDAAPAEEAAAEEEEEsDDDmGFGLFD.[-] N-Term(TMT6plex); S30(Phospho); M34(Oxidation) 1 P05317 P05317 0 4 0.3822 0 1 1 1062.19535 4245.75959 4245.73286 6.29 0.00668 721050.25 CID (Collision Induced Dissociation) MS2 14.01607 50 17.5 60.0 137.6753 46957 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 3.9 16.6 25.0 1.9 15.4 35.4 8.8 19.6 33.3 15.1 Excluded by Method 4.84 1 0.0003003 0.0003435 False High Sequest HT (A2) Unambiguous ALVLELDEMSNGSEIQDALEEISGQK [K].aLVLELDEmsNGSEIQDALEEISGQk.[T] N-Term(TMT6plex); M9(Oxidation); S10(Phospho); K26(TMT6plex) 2 P17695 P17695; P17695-2 0 4 0.0829 0 1 1 843.92001 3372.65822 3372.65337 1.44 0.00121 614438.9375 CID (Collision Induced Dissociation) MS2 30.34212 80 48.1 60.0 137.3312 46837 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 20.3 45.4 69.6 18.6 44.8 74.9 26.6 45.5 79.0 56.0 4.22 1 0.0003003 0.0001574 False High Sequest HT (A2) Unambiguous VVDYSQFQESDDADEDYGRDSGPPTK [K].vVDYSQFQEsDDADEDYGRDSGPPTk.[K] N-Term(TMT6plex); S10(Phospho); K26(TMT6plex) 1 Q9H1E3 Q9H1E3 1 4 0.0956 0 1 1 865.38873 3458.5331 3458.53134 0.51 0.00044 438082.8125 CID (Collision Induced Dissociation) MS2 9.409125 50 8.4 60.0 137.0633 46747 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 6.8 6.8 12.9 3.6 8.7 9.5 8.4 6.5 13.6 7.7 Excluded by Method 4.08 1 0.0003003 3.743e-05 False High Sequest HT (A2) Unambiguous EEEAAEESDDDMGFGLFD [K].eEEAAEEsDDDMGFGLFD.[-] N-Term(TMT6plex); S8(Phospho) 2 P05319 P10622; P05319 0 3 0.7782 0 1 1 772.29724 2314.87718 2314.8784 -0.53 -0.00041 730958.5625 CID (Collision Induced Dissociation) MS2 13.9111 80 37.4 60.0 136.8515 46674 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 9.4 35.1 58.3 14.7 28.1 66.2 16.2 36.2 71.8 38.5 5.05 1 0.0003003 2.19e-05 False High Sequest HT (A2) Unambiguous YAAAAPAATSAASGDAAPAEEAAAEEEEESDDDMGFGLFD [K].yAAAAPAATSAASGDAAPAEEAAAEEEEEsDDDmGFGLFD.[-] N-Term(TMT6plex); S30(Phospho); M34(Oxidation) 1 P05317 P05317 0 4 0.3768 0 1 1 1062.19377 4245.75324 4245.73286 4.8 0.00509 848256.0 CID (Collision Induced Dissociation) MS2 13.21228 50 19.6 60.0 136.3703 46504 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 2.6 15.6 26.1 6.9 13.5 43.8 7.1 16.3 40.4 23.2 Excluded by Method 4.83 1 0.0003003 0.0001375 False High Sequest HT (A2) Unambiguous VQGEAVSNIQENTQTPTVQEESEEEEVDETGVEVK [K].vQGEAVSNIQENTQTPTVQEEsEEEEVDETGVEVk.[D] N-Term(TMT6plex); S22(Phospho); K35(TMT6plex) 3 Q13765 Q13765; E9PAV3; E9PAV3-2 0 4 0.0328 0 1 1 1100.52184 4399.06553 4399.06016 1.22 0.00134 463355.8125 CID (Collision Induced Dissociation) MS2 5.610644 40 19.3 60.0 136.0485 46394 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 22.6 17.3 14.7 18.9 17.9 16.9 20.0 23.7 19.3 21.3 Excluded by Method 4.88 1 0.0003003 2.689e-05 False High Sequest HT (A2) Unambiguous EEEAAEESDDDMGFGLFD [K].eEEAAEEsDDDMGFGLFD.[-] N-Term(TMT6plex); S8(Phospho) 2 P05319 P10622; P05319 0 3 0.8013 0 1 1 772.29773 2314.87864 2314.8784 0.1 8e-05 1040338.5 CID (Collision Induced Dissociation) MS2 0.0 80 37.2 60.0 135.7891 46305 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 10.1 31.4 61.3 10.8 31.1 65.6 21.8 37.7 73.3 29.3 4.73 1 0.0003003 7.448e-06 False High Sequest HT (A2) Unambiguous VQGEAVSNIQENTQTPTVQEESEEEEVDETGVEVK [K].vQGEAVSNIQENTQTPTVQEEsEEEEVDETGVEVk.[D] N-Term(TMT6plex); S22(Phospho); K35(TMT6plex) 3 Q13765 Q13765; E9PAV3; E9PAV3-2 0 4 0.0319 0 1 1 1100.51647 4399.04405 4399.06016 -3.66 -0.00403 396282.90625 CID (Collision Induced Dissociation) MS2 8.332326 40 11.1 60.0 138.2774 47166 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 8.3 10.1 7.9 14.7 12.2 11.5 10.6 13.3 11.5 11.0 Excluded by Method 4.39 1 0.0003003 0.0001669 False High Sequest HT (A2) Unambiguous YAAAAPAATSAASGDAAPAEEAAAEEEEESDDDMGFGLFD [K].yAAAAPAATSAASGDAAPAEEAAAEEEEEsDDDmGFGLFD.[-] N-Term(TMT6plex); S30(Phospho); M34(Oxidation) 1 P05317 P05317 0 4 0.2275 0 1 1 1062.1923 4245.74738 4245.73286 3.42 0.00363 814199.5 CID (Collision Induced Dissociation) MS2 0.0 60 14.1 60.0 138.7705 47335 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 1.3 15.2 24.1 2.3 11.2 30.0 4.1 12.9 26.2 13.4 Excluded by Method 4.44 1 0.0003003 0.0002435 False High Sequest HT (A2) Unambiguous ALVLELDEMSNGSEIQDALEEISGQK [K].aLVLELDEmsNGSEIQDALEEISGQk.[T] N-Term(TMT6plex); M9(Oxidation); S10(Phospho); K26(TMT6plex) 2 P17695 P17695; P17695-2 0 4 0.0909 0 1 1 843.92166 3372.66481 3372.65337 3.39 0.00286 340796.71875 CID (Collision Induced Dissociation) MS2 27.49573 60 31.6 60.0 142.1918 48516 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 7.8 26.4 44.6 13.0 26.7 59.1 13.1 32.5 57.2 35.9 Excluded by Method 4.51 1 0.0003003 0.0002956 False High Sequest HT (A2) Unambiguous EEEAAEESDDDMGFGLFD [K].eEEAAEEsDDDMGFGLFD.[-] N-Term(TMT6plex); S8(Phospho) 2 P05319 P10622; P05319 0 3 0.7674 0 1 1 772.29724 2314.87718 2314.8784 -0.53 -0.00041 377271.53125 CID (Collision Induced Dissociation) MS2 14.72376 50 27.8 60.0 141.4062 48243 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 10.1 23.4 44.1 6.4 18.5 58.0 7.3 27.0 57.5 26.0 Excluded by Method 4.6 1 0.0003003 1.022e-05 False High Sequest HT (A2) Unambiguous EEEAAEESDDDMGFGLFD [K].eEEAAEEsDDDmGFGLFD.[-] N-Term(TMT6plex); S8(Phospho); M12(Oxidation) 2 P05319 P10622; P05319 0 3 0.6715 0 1 1 777.63067 2330.87746 2330.87332 1.78 0.00138 1842399.5 CID (Collision Induced Dissociation) MS2 35.68966 40 151.6 44.579 140.9674 48094 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 38.8 120.3 242.5 46.6 124.5 297.9 48.5 138.1 310.0 148.6 Excluded by Method 3.47 1 0.0003003 0.0005207 False High Sequest HT (A2) Unambiguous ALVLELDEMSNGSEIQDALEEISGQK [K].aLVLELDEmsNGSEIQDALEEISGQk.[T] N-Term(TMT6plex); M9(Oxidation); S10(Phospho); K26(TMT6plex) 2 P17695 P17695; P17695-2 0 4 0.0739 0 1 1 843.91855 3372.65236 3372.65337 -0.3 -0.00025 440794.875 CID (Collision Induced Dissociation) MS2 5.818029 70 37.4 60.0 138.3822 47201 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 15.5 34.5 53.5 18.0 27.2 62.6 21.7 44.7 62.5 34.0 4.06 1 0.0003003 0.000421 False High Sequest HT (A2) Unambiguous YAAAAPAATSAASGDAAPAEEAAAEEEEESDDDMGFGLFD [K].yAAAAPAATSAASGDAAPAEEAAAEEEEEsDDDmGFGLFD.[-] N-Term(TMT6plex); S30(Phospho); M34(Oxidation) 1 P05317 P05317 0 4 0.272 0 1 1 1062.19206 4245.7464 4245.73286 3.19 0.00338 740712.9375 CID (Collision Induced Dissociation) MS2 0.0 70 17.7 60.0 140.3264 47872 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 3.7 14.8 25.9 3.1 12.7 33.2 8.4 16.9 37.8 20.5 4.78 1 0.0003003 0.0002208 False High Sequest HT (A2) Unambiguous EEEAAEESDDDMGFGLFD [K].eEEAAEEsDDDMGFGLFD.[-] N-Term(TMT6plex); S8(Phospho) 2 P05319 P10622; P05319 0 3 0.8535 0 1 1 772.29761 2314.87828 2314.8784 -0.05 -4e-05 517331.1875 CID (Collision Induced Dissociation) MS2 4.948272 80 41.1 60.0 138.9956 47413 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 11.0 35.6 57.8 14.2 31.2 83.2 15.6 39.2 79.0 44.5 3.89 1 0.0003003 0.0001973 False High Sequest HT (A2) Unambiguous EEEAAEESDDDMGFGLFD [K].eEEAAEEsDDDMGFGLFD.[-] N-Term(TMT6plex); S8(Phospho) 2 P05319 P10622; P05319 0 3 0.6553 0 1 1 772.29724 2314.87718 2314.8784 -0.53 -0.00041 336013.71875 CID (Collision Induced Dissociation) MS2 4.408593 80 31.4 60.0 140.2432 47843 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 4.2 27.2 41.2 7.6 19.2 66.2 15.5 32.0 67.6 33.6 4.09 1 0.0003003 0.0001835 False High Sequest HT (A2) Unambiguous YAAAAPAATSAASGDAAPAEEAAAEEEEESDDDMGFGLFD [K].yAAAAPAATSAASGDAAPAEEAAAEEEEEsDDDmGFGLFD.[-] N-Term(TMT6plex); S30(Phospho); M34(Oxidation) 1 P05317 P05317 0 4 0.3909 0 1 1 1062.19389 4245.75373 4245.73286 4.91 0.00522 688139.75 CID (Collision Induced Dissociation) MS2 7.230999 40 13.6 60.0 135.2941 46132 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 3.3 10.7 23.3 11.7 24.8 6.9 13.2 27.9 13.9 Excluded by Method 5.27 1 0.0003003 3.792e-05 False High Sequest HT (A2) Unambiguous EEEAAEESDDDMGFGLFD [K].eEEAAEEsDDDmGFGLFD.[-] N-Term(TMT6plex); S8(Phospho); M12(Oxidation) 2 P05319 P10622; P05319 0 3 0.7168 0 1 1 777.63031 2330.87636 2330.87332 1.31 0.00102 5609621.5 CID (Collision Induced Dissociation) MS2 29.76866 40 63.0 13.327 130.6114 44340 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 27.4 51.3 96.7 26.3 52.9 115.4 32.1 55.1 114.3 58.7 Excluded by Method 3.39 1 0.0003003 0.0005329 False High Sequest HT (A2) Unambiguous YAAAAPAATSAASGDAAPAEEAAAEEEEESDDDMGFGLFD [K].yAAAAPAATSAASGDAAPAEEAAAEEEEEsDDDmGFGLFD.[-] N-Term(TMT6plex); S30(Phospho); M34(Oxidation) 1 P05317 P05317 0 4 0.2829 0 1 1 1062.19084 4245.74152 4245.73286 2.04 0.00216 2261144.0 CID (Collision Induced Dissociation) MS2 19.13173 60 19.0 19.594 131.1939 44592 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 7.4 18.1 30.1 6.6 14.4 31.1 7.1 18.7 37.4 18.7 Excluded by Method 4.1 1 0.0003003 0.0003288 False High Sequest HT (A2) Unambiguous SLQPFDDLLGDFNK [K].sLQPFDDLLGDFNk.[V] N-Term(TMT6plex); S1(Phospho); K14(TMT6plex) 1 Q06336 Q06336 0 3 0.4411 0 1 1 716.36611 2147.08376 2147.08261 0.54 0.00039 1791553.375 CID (Collision Induced Dissociation) MS2 0.0 40 93.1 49.131 130.4947 44290 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 23.8 77.8 134.1 39.4 86.0 167.0 36.8 93.6 176.8 95.2 Excluded by Method 3.31 1 0.0003003 0.0003535 False High Sequest HT (A2) Unambiguous DSTLIMQLLRDNLTLWTSENQGDEGDAGEGEN [K].dSTLImQLLRDNLTLWTSENQGDEGDAGEGEN.[-] N-Term(TMT6plex); M6(Oxidation) 2 P31946 P31946-2; P31946 1 4 0.3087 0 1 1 942.44576 3766.7612 3766.75512 1.61 0.00152 2471128.25 CID (Collision Induced Dissociation) MS2 3.561391 30 64.1 21.667 130.4403 44267 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 74.6 66.4 46.2 60.2 52.2 68.9 71.5 72.3 68.0 60.3 Excluded by Method 3.79 1 0.0003003 4.63e-05 False High Sequest HT (A2) Unambiguous EEEAAEESDDDMGFGLFD [K].eEEAAEEsDDDmGFGLFD.[-] N-Term(TMT6plex); S8(Phospho); M12(Oxidation) 2 P05319 P10622; P05319 0 2 0.7678 0 1 1 1165.94228 2330.87729 2330.87332 1.71 0.00199 6810151.5 CID (Collision Induced Dissociation) MS2 24.3838 70 37.4 13.236 130.0848 44110 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 9.5 28.4 59.0 14.0 25.3 72.3 12.0 39.1 69.2 45.4 4.22 1 0.0003003 0.0005169 False High Sequest HT (A2) Unambiguous ALVLELDEMSNGSEIQDALEEISGQK [K].aLVLELDEmsNGSEIQDALEEISGQk.[T] N-Term(TMT6plex); M9(Oxidation); S10(Phospho); K26(TMT6plex) 2 P17695 P17695; P17695-2 0 3 0.033 0 1 1 1124.88961 3372.65426 3372.65337 0.26 0.0003 4687362.0 CID (Collision Induced Dissociation) MS2 7.685104 60 41.2 23.189 129.9728 44061 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 14.6 34.0 59.7 21.4 34.4 73.6 17.1 39.0 81.7 36.4 Excluded by Method 3.03 1 0.0003003 8.123e-05 False High Sequest HT (A2) Unambiguous EEEAAEESDDDMGFGLFD [K].eEEAAEEsDDDMGFGLFD.[-] N-Term(TMT6plex); S8(Phospho) 2 P05319 P10622; P05319 0 2 0.8344 0 1 1 1157.94522 2314.88317 2314.8784 2.06 0.00239 2773474.5 CID (Collision Induced Dissociation) MS2 27.83135 60 53.7 37.436 130.4924 44289 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 13.6 47.1 78.6 12.3 39.6 110.6 21.0 47.5 107.3 59.0 Excluded by Method 4.71 1 0.0003003 4.044e-07 False High Sequest HT (A2) Unambiguous EEEAAEESDDDMGFGLFD [K].eEEAAEEsDDDMGFGLFD.[-] N-Term(TMT6plex); S8(Phospho) 2 P05319 P10622; P05319 0 3 0.5464 0 1 1 772.29779 2314.87883 2314.8784 0.18 0.00014 1744929.75 CID (Collision Induced Dissociation) MS2 5.962229 30 26.5 49.246 131.6391 44778 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 8.0 19.7 37.6 10.7 17.9 45.6 14.8 22.1 60.1 29.0 Excluded by Method 3.88 1 0.0003003 3.462e-05 False High Sequest HT (A2) Unambiguous ALVLELDEMSNGSEIQDALEEISGQK [K].aLVLELDEmsNGSEIQDALEEISGQk.[T] N-Term(TMT6plex); M9(Oxidation); S10(Phospho); K26(TMT6plex) 2 P17695 P17695; P17695-2 0 4 0.0773 0 1 1 843.92013 3372.65871 3372.65337 1.58 0.00133 873026.5 CID (Collision Induced Dissociation) MS2 16.08156 40 53.5 60.0 134.8967 45991 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 24.3 47.5 70.9 21.1 44.3 93.0 26.4 59.5 91.9 55.6 Excluded by Method 4.4 1 0.0003003 0.0003668 False High Sequest HT (A2) Unambiguous VQGEAVSNIQENTQTPTVQEESEEEEVDETGVEVK [K].vQGEAVSNIQENTQTPTVQEEsEEEEVDETGVEVk.[D] N-Term(TMT6plex); S22(Phospho); K35(TMT6plex) 3 Q13765 Q13765; E9PAV3; E9PAV3-2 0 4 0.0642 0 1 1 1100.5194 4399.05577 4399.06016 -1.0 -0.0011 476326.75 CID (Collision Induced Dissociation) MS2 0.0 50 24.5 60.0 133.5062 45498 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 21.7 27.2 21.7 21.8 21.7 26.8 29.2 25.1 25.5 24.4 Excluded by Method 5.3 1 0.0003003 1.481e-05 False High Sequest HT (A2) Unambiguous FALGQGVGVILCIGETLEEK [K].fALGQGVGVILcIGETLEEk.[K] N-Term(TMT6plex); C12(Carbamidomethyl); K20(TMT6plex) 1 P00942 P00942 0 3 0.5429 0 1 1 864.4884 2591.45066 2591.45188 -0.47 -0.00041 6378833.5 CID (Collision Induced Dissociation) MS2 6.569409 60 32.0 16.371 131.6191 44770 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 11.3 32.3 42.1 12.1 23.7 51.9 15.5 35.8 61.6 33.3 Excluded by Method 3.61 1 0.0003003 0.000125 False High Sequest HT (A2) Unambiguous YAAAAPAATSAASGDAAPAEEAAAEEEEESDDDMGFGLFD [K].yAAAAPAATSAASGDAAPAEEAAAEEEEEsDDDmGFGLFD.[-] N-Term(TMT6plex); S30(Phospho); M34(Oxidation) 1 P05317 P05317 0 4 0.3511 0 1 1 1062.19389 4245.75373 4245.73286 4.91 0.00522 1270379.25 CID (Collision Induced Dissociation) MS2 8.798223 60 29.2 59.989 132.8988 45272 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 7.6 25.9 46.8 7.9 22.7 59.6 12.1 27.5 52.1 29.6 Excluded by Method 4.7 1 0.0003003 8.793e-05 False High Sequest HT (A2) Unambiguous EYLPQINDIDNQISSLLNEVTIIK [K].eYLPQINDIDNQISsLLNEVTIIk.[Q] N-Term(TMT6plex); S15(Phospho); K24(TMT6plex) 1 P53278 P53278 0 4 0.0313 0 1 1 828.44445 3310.75596 3310.75876 -0.85 -0.0007 954985.4375 CID (Collision Induced Dissociation) MS2 60.10824 40 94.5 50.974 132.3877 45078 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 28.7 82.5 154.9 28.9 66.6 180.6 42.3 80.7 189.9 89.6 Excluded by Method 4.47 1 0.0003003 5.313e-05 False High Sequest HT (A2) Unambiguous VQGEAVSNIQENTQTPTVQEESEEEEVDETGVEVK [K].vQGEAVSNIQENTQTPTVQEEsEEEEVDETGVEVk.[D] N-Term(TMT6plex); S22(Phospho); K35(TMT6plex) 3 Q13765 Q13765; E9PAV3; E9PAV3-2 0 4 0.0869 0 1 1 1100.51818 4399.05088 4399.06016 -2.11 -0.00232 1580609.0 CID (Collision Induced Dissociation) MS2 7.70158 60 26.1 49.073 132.2542 45025 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 20.4 30.7 25.8 24.8 22.2 24.0 31.1 32.2 23.5 25.8 Excluded by Method 5.18 1 0.0003003 8.587e-07 False High Sequest HT (A2) Unambiguous EEEAAEESDDDMGFGLFD [K].eEEAAEEsDDDMGFGLFD.[-] N-Term(TMT6plex); S8(Phospho) 2 P05319 P10622; P05319 0 3 0.8497 0 1 1 772.29767 2314.87846 2314.8784 0.03 2e-05 1580777.5 CID (Collision Induced Dissociation) MS2 44.27884 80 36.0 60.0 132.7828 45231 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 10.5 32.4 55.9 10.9 31.8 61.4 13.0 35.4 71.9 36.4 5.59 1 0.0003003 4.888e-06 False High Sequest HT (A2) Unambiguous EEEAAEESDDDMGFGLFD [K].eEEAAEEsDDDMGFGLFD.[-] N-Term(TMT6plex); S8(Phospho) 2 P05319 P10622; P05319 0 3 0.8064 0 1 1 772.2984 2314.88066 2314.8784 0.97 0.00075 286801.65625 CID (Collision Induced Dissociation) MS2 8.177386 60 27.8 60.0 142.4625 48609 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 4.0 24.5 46.0 10.0 22.3 50.5 12.9 24.8 56.7 26.6 Excluded by Method 4.39 1 0.0003003 1.153e-05 False High Sequest HT (A2) Unambiguous ALVLELDEMSNGSEIQDALEEISGQK [K].aLVLELDEmsNGSEIQDALEEISGQk.[T] N-Term(TMT6plex); M9(Oxidation); S10(Phospho); K26(TMT6plex) 2 P17695 P17695; P17695-2 0 4 0.0419 0 1 1 843.92068 3372.6609 3372.65337 2.23 0.00188 500203.78125 CID (Collision Induced Dissociation) MS2 56.32531 40 33.9 60.0 143.2937 48897 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 15.1 33.7 45.3 15.5 26.2 60.6 16.8 31.2 60.8 34.3 Excluded by Method 4.06 1 0.0003003 0.0004188 False High Sequest HT (A2) Unambiguous DGQTDSAAEESSSQETSK [K].dGQTDsAAEESSSQETSk.[GD] N-Term(TMT6plex); S6(Phospho); K18(TMT6plex) 4 P55201 P55201-3; P55201-4; P55201-2; P55201 0 3 0.0227 0 1 1 799.02321 2395.05508 2395.05501 0.03 2e-05 826503.3125 CID (Collision Induced Dissociation) MS2 0.0 60 21.8 60.0 43.9744 11755 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 20.3 19.6 19.1 26.1 21.2 19.7 26.7 18.9 23.1 23.4 Excluded by Method 4.84 1 0.0003003 1.598e-06 False High Sequest HT (A2) Unambiguous HIVSNDSSDSDDESHEPK [K].hIVSNDSSDsDDESHEPk.[G] N-Term(TMT6plex); S10(Phospho); K18(TMT6plex) 1 P47712 P47712 0 3 0.1286 0 1 1 846.04638 2536.12458 2536.12409 0.19 0.00016 1340404.375 CID (Collision Induced Dissociation) MS2 0.0 40 80.9 60.0 43.2223 11483 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 51.1 78.0 85.0 74.2 75.4 96.8 80.5 75.8 137.5 54.3 Excluded by Method 6.3 1 0.0003003 3.588e-06 False High Sequest HT (A2) Unambiguous ANADANEDNNVDEK [K].aNADANEDNNVDEk.[Q] N-Term(TMT6plex); K14(TMT6plex) 1 P49090 P49090 0 3 0.5231 0 1 1 659.65676 1976.95574 1976.95615 -0.21 -0.00014 3517970.0 CID (Collision Induced Dissociation) MS2 0.0 80 93.5 60.0 43.0035 11402 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 21.4 73.2 124.0 32.3 75.3 185.2 42.3 86.3 191.9 102.9 3.9 1 0.0003003 2.7e-06 False High Sequest HT (A2) Unambiguous RGSIGENQGEEK [K].rGsIGENQGEEk.[G] N-Term(TMT6plex); S3(Phospho); K12(TMT6plex) 3 Q05682-2 Q05682-3; Q05682-6; Q05682-2 1 3 0.5346 0 1 1 614.64384 1841.91698 1841.91588 0.6 0.00037 9188061.0 CID (Collision Induced Dissociation) MS2 5.099119 60 141.6 36.398 39.8855 10277 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 140.4 140.2 106.9 154.7 139.7 132.7 156.0 161.1 142.5 141.9 Excluded by Method 5.2 1 0.0003003 5.782e-06 False High Sequest HT (A2) Unambiguous YAAAAPAATSAASGDAAPAEEAAAEEEEESDDDMGFGLFD [K].yAAAAPAATSAASGDAAPAEEAAAEEEEEsDDDmGFGLFD.[-] N-Term(TMT6plex); S30(Phospho); M34(Oxidation) 1 P05317 P05317 0 4 0.3327 0 1 1 1062.19377 4245.75324 4245.73286 4.8 0.00509 697733.0625 CID (Collision Induced Dissociation) MS2 11.73902 40 11.7 60.0 146.299 49939 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 1.6 8.1 17.7 2.0 8.9 23.0 2.1 12.0 27.3 13.9 Excluded by Method 5.05 1 0.0003003 2.19e-05 False High Sequest HT (A2) Unambiguous EEEAAEESDDDMGFGLFD [K].eEEAAEEsDDDMGFGLFD.[-] N-Term(TMT6plex); S8(Phospho) 2 P05319 P10622; P05319 0 2 0.6657 0 1 1 1157.94938 2314.89147 2314.8784 5.65 0.00654 583105.1875 CID (Collision Induced Dissociation) MS2 53.62085 50 48.2 60.0 146.2384 49918 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 12.1 38.7 75.1 14.5 37.7 94.4 19.6 44.9 91.5 53.0 Excluded by Method 3.47 1 0.0003003 0.0003998 False High Sequest HT (A2) Unambiguous EEEAAEESDDDMGFGLFD [K].eEEAAEEsDDDMGFGLFD.[-] N-Term(TMT6plex); S8(Phospho) 2 P05319 P10622; P05319 0 3 0.8507 0 1 1 772.29944 2314.88377 2314.8784 2.32 0.00179 483755.75 CID (Collision Induced Dissociation) MS2 5.954472 50 39.2 60.0 143.7779 49066 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 9.1 30.2 61.2 10.5 29.5 78.3 13.5 38.0 78.6 42.8 Excluded by Method 3.75 1 0.0003003 0.0003095 False High Sequest HT (A2) Unambiguous YAAAAPAATSAASGDAAPAEEAAAEEEEESDDDMGFGLFD [K].yAAAAPAATSAASGDAAPAEEAAAEEEEEsDDDmGFGLFD.[-] N-Term(TMT6plex); S30(Phospho); M34(Oxidation) 1 P05317 P05317 0 4 0.398 0 1 1 1062.1934 4245.75177 4245.73286 4.45 0.00473 517582.25 CID (Collision Induced Dissociation) MS2 0.0 50 16.7 60.0 147.5479 50368 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 2.8 15.3 29.3 4.1 13.2 29.6 8.3 16.3 34.1 13.6 Excluded by Method 5.1 1 0.0003003 7.763e-05 False High Sequest HT (A2) Unambiguous NAEEESESEAEEGD [K].nAEEEsEsEAEEGD.[-] N-Term(TMT6plex); S6(Phospho); S8(Phospho) 4 Q7L1Q6 Q7L1Q6-2; Q7L1Q6-4; Q7L1Q6-3; Q7L1Q6 0 2 0.8247 0 1 1 957.32529 1913.6433 1913.6412 1.1 0.00105 230933.53125 CID (Collision Induced Dissociation) MS2 0.0 40 13.4 60.0 37.805 9522 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 10.8 15.4 10.8 11.8 8.1 15.1 20.6 13.5 17.1 11.0 Excluded by Method 2.91 1 0.0003003 0.0003263 False High Sequest HT (A2) Unambiguous HSPTEDEESAK [K].hsPTEDEESAk.[A] N-Term(TMT6plex); S2(Phospho); K11(TMT6plex) 1 P46821 P46821 0 3 0.0048 0 1 1 589.94485 1767.82001 1767.82025 -0.13 -8e-05 154474.40625 CID (Collision Induced Dissociation) MS2 0.0 20 59.5 60.0 34.6996 8409 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 50.1 58.0 51.1 68.9 57.5 59.6 70.5 61.5 62.8 55.3 Excluded by Method 4.17 1 0.0003003 0.0001185 False High Sequest HT (A2) Unambiguous NSQEDSEDSEDK [K].nSQEDsEDSEDk.[D] N-Term(TMT6plex); S6(Phospho); K12(TMT6plex) 1 Q9H1E3 Q9H1E3 0 3 0.126 0 1 1 640.94275 1920.81369 1920.8112 1.3 0.00083 91887.6640625 CID (Collision Induced Dissociation) MS2 0.0 60 37.9 60.0 33.6753 8043 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 36.6 39.5 31.5 42.7 33.0 33.2 51.6 36.2 41.3 33.4 Excluded by Method 3.73 1 0.0003003 0.0003024 False High Sequest HT (A2) Unambiguous GILAADESTGSIAK [K].gILAADEsTGSIAk.[R] N-Term(TMT6plex); S8(Phospho); K14(TMT6plex) 2 P04075 P04075; P04075-2 0 2 0.0505 0 1 1 936.00019 1870.99311 1870.99273 0.2 0.00019 573374.9375 CID (Collision Induced Dissociation) MS2 0.0 40 31.6 60.0 93.9495 30400 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 32.2 28.2 22.7 31.8 28.2 37.8 34.6 35.2 35.7 29.4 Excluded by Method 3.96 1 0.0003003 0.0004201 False High Sequest HT (A2) Unambiguous RTADSSSSEDEEEYVVEK [K].rTADSSSsEDEEEYVVEk.[V] N-Term(TMT6plex); S8(Phospho); K18(TMT6plex) 1 P45973 P45973 1 3 0.0468 0 1 1 866.73372 2598.1866 2598.18602 0.22 0.00019 875162.25 CID (Collision Induced Dissociation) MS2 8.006186 60 109.2 60.0 68.0709 20686 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 112.8 112.6 91.1 111.1 113.1 103.9 116.6 115.9 103.4 111.9 Excluded by Method 5.13 1 0.0003003 6.094e-06 False High Sequest HT (A2) Unambiguous NTSSPPISPNAAAIQEEDSSK [K].nTSsPPIsPNAAAIQEEDSSk.[K] N-Term(TMT6plex); S4(Phospho); S8(Phospho); K21(TMT6plex) 1 P40091 P40091 0 3 0.1408 0 1 1 921.09605 2761.27359 2761.27347 0.04 4e-05 1222309.875 CID (Collision Induced Dissociation) MS2 2.843914 40 100.8 60.0 85.7512 27325 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 75.5 93.5 108.6 76.2 92.6 122.9 89.9 105.3 135.3 108.2 Excluded by Method 7.1 1 0.0003003 2.362e-06 False High Sequest HT (A2) Unambiguous EESVGSDDDATAANQQVYLK [K].eESVGsDDDATAANQQVYLk.[T] N-Term(TMT6plex); S6(Phospho); K20(TMT6plex) 1 Q03305 Q03305 0 3 0.0204 0 1 1 893.42636 2678.26452 2678.25986 1.74 0.00156 2698316.75 CID (Collision Induced Dissociation) MS2 61.40898 90 63.9 41.022 85.1245 27094 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 18.0 50.1 102.6 26.8 46.9 116.0 32.5 50.9 124.2 70.8 6.37 1 0.0003003 6.257e-08 False High Sequest HT (A2) Unambiguous VLDEEGSEREFDEDSDEK [K].vLDEEGsEREFDEDsDEk.[E] N-Term(TMT6plex); S7(Phospho); S15(Phospho); K18(TMT6plex) 1 O43719 O43719 1 3 0.6297 0 1 1 916.05297 2746.14434 2746.14218 0.79 0.00072 459852.75 CID (Collision Induced Dissociation) MS2 47.19191 40 277.7 60.0 84.8826 27003 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 272.0 284.0 214.9 316.4 273.0 257.8 322.2 302.9 281.1 252.8 Excluded by Method 6.4 1 0.0003003 6.056e-06 False High Sequest HT (A2) Unambiguous LFEESDDKEDEDADGK [K].lFEEsDDkEDEDADGk.[E] N-Term(TMT6plex); S5(Phospho); K8(TMT6plex); K16(TMT6plex) 1 O43719 O43719 1 3 0.4094 0 1 1 870.40827 2609.21027 2609.21106 -0.31 -0.00027 561238.0625 CID (Collision Induced Dissociation) MS2 0.0 50 365.7 60.0 86.0095 27423 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 376.8 361.5 236.2 517.7 356.2 286.9 458.5 364.7 373.7 324.9 Excluded by Method 3.42 1 0.0003003 0.0002509 False High Sequest HT (A2) Unambiguous GPTTGEGALDLSDVHSPPKSPEGK [K].gPTTGEGALDLSDVHsPPksPEGk.[T] N-Term(TMT6plex); S16(Phospho); K19(TMT6plex); S20(Phospho); K24(TMT6plex) 2 O95684 O95684-2; O95684 1 4 0.5299 0 1 1 806.65363 3223.59268 3223.58922 1.08 0.00087 1234514.625 CID (Collision Induced Dissociation) MS2 0.0 40 106.3 50.406 87.0018 27799 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 116.9 104.0 90.7 115.1 100.6 95.7 115.1 101.4 117.6 106.2 Excluded by Method 7.53 1 0.0003003 2.094e-05 False High Sequest HT (A2) Unambiguous LPRTQPDGTSVPGEPASPISQRLPPK [K].lPRTQPDGTSVPGEPAsPISQRLPPk.[V] N-Term(TMT6plex); S17(Phospho); K26(TMT6plex) 3 Q14980 Q14980-5; Q14980-2; Q14980 2 4 0.1336 0 1 1 816.69353 3263.75228 3263.75535 -0.94 -0.00077 1910502.625 CID (Collision Induced Dissociation) MS2 4.111108 50 56.1 60.0 86.9068 27763 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 59.2 57.8 47.0 61.2 57.0 52.6 69.1 53.1 50.7 53.8 Excluded by Method 5.09 1 0.0003003 0.0002512 False High Sequest HT (A2) Unambiguous LFEDDDSNEK [K].lFEDDDsNEk.[L] N-Term(TMT6plex); S7(Phospho); K10(TMT6plex) 1 O43719 O43719 0 3 0.481 0 1 1 583.9373 1749.79734 1749.79845 -0.64 -0.00037 643542.3125 CID (Collision Induced Dissociation) MS2 23.8532 60 152.3 60.0 86.8688 27750 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 165.8 138.6 109.3 181.2 152.8 130.0 197.7 159.8 148.1 139.9 Excluded by Method 4.22 1 0.0003003 5.708e-05 False High Sequest HT (A2) Unambiguous SPGSAGRSQTPPGVATPPIPK [K].sPGsAGRSQTPPGVATPPIPk.[I] N-Term(TMT6plex); S1(Phospho); S4(Phospho); K21(TMT6plex) 1 Q15648 Q15648 1 3 0.0524 0 1 1 874.11304 2620.32457 2620.33014 -2.13 -0.00186 594634.8125 CID (Collision Induced Dissociation) MS2 48.04169 60 52.4 60.0 86.6467 27663 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 61.4 57.4 45.9 47.8 50.9 55.3 58.3 52.1 48.6 45.9 Excluded by Method 4.01 1 0.0003003 0.00017 False High Sequest HT (A2) Unambiguous SPSDSGYSYETIGK [K].sPsDSGYSYETIGk.[T] N-Term(TMT6plex); S3(Phospho); K14(TMT6plex) 1 P46821 P46821 0 3 0.0238 0 1 1 676.99047 2028.95686 2028.95674 0.06 4e-05 15448054.0 CID (Collision Induced Dissociation) MS2 4.446633 20 79.4 13.401 82.5499 26122 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 87.5 73.1 62.8 84.8 81.3 86.3 79.1 83.6 75.6 80.1 Excluded by Method 2.94 1 0.0003003 6.352e-05 False High Sequest HT (A2) Unambiguous VQSLEGEKLSPK [K].vQSLEGEkLsPk.[S] N-Term(TMT6plex); K8(TMT6plex); S10(Phospho); K12(TMT6plex) 1 P46821 P46821 1 4 0.571 0 1 1 521.30075 2082.18118 2082.18148 -0.15 -8e-05 2521485.75 CID (Collision Induced Dissociation) MS2 0.833776 40 199.1 40.365 82.541 26118 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 188.1 192.8 135.8 244.8 198.1 169.0 233.3 213.9 204.0 211.3 Excluded by Method 6.48 1 0.0003003 5.779e-07 False High Sequest HT (A2) Unambiguous ADDEEDLSDENIQPELRGQ [K].aDDEEDLsDENIQPELRGQ.[-] N-Term(TMT6plex); S8(Phospho) 1 P14164 P14164 1 3 0.5526 0 1 1 828.03219 2482.08202 2482.082 0.01 1e-05 13694950.0 CID (Collision Induced Dissociation) MS2 1.465979 50 77.0 10.27 82.5393 26117 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 21.7 60.8 126.7 22.7 65.0 148.5 24.7 71.4 150.3 78.3 Excluded by Method 5.61 1 0.0003003 1.498e-05 False High Sequest HT (A2) Unambiguous VEMYSGSDDDDDFNK [K].vEmYsGsDDDDDFNk.[L] N-Term(TMT6plex); M3(Oxidation); S5(Phospho); S7(Phospho); K15(TMT6plex) 1 O60841 O60841 0 3 0.1355 0 1 1 790.97556 2370.91211 2370.91264 -0.22 -0.00018 608872.625 CID (Collision Induced Dissociation) MS2 12.90742 30 73.9 60.0 82.4687 26092 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 70.2 64.4 64.6 82.3 69.9 69.0 85.0 71.3 82.9 79.3 Excluded by Method 3.91 1 0.0003003 0.0002011 False High Sequest HT (A2) Unambiguous ELEENDSENSEFEDDGSEK [K].eLEENDsENsEFEDDGSEk.[V] N-Term(TMT6plex); S7(Phospho); S10(Phospho); K19(TMT6plex) 1 O43719 O43719 0 3 0.5829 0 1 1 940.70801 2820.10948 2820.10619 1.17 0.0011 467788.3125 CID (Collision Induced Dissociation) MS2 11.80973 40 43.1 60.0 81.7109 25805 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 37.1 30.5 46.2 42.4 48.2 51.3 41.3 42.2 53.4 37.9 Excluded by Method 6.09 1 0.0003003 7.568e-07 False High Sequest HT (A2) Unambiguous EYVPLDNAEQSTSSSQETK [K].eYVPLDNAEQSTSSsQETk.[E] N-Term(TMT6plex); S15(Phospho); K19(TMT6plex) 1 P33322 P33322 0 3 0.0167 0 1 1 884.42113 2651.24883 2651.24896 -0.05 -4e-05 946741.3125 CID (Collision Induced Dissociation) MS2 15.86301 80 138.7 60.0 82.951 26271 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 43.6 102.5 211.9 47.4 124.0 264.6 62.0 122.7 272.6 135.9 5.99 1 0.0003003 5.557e-07 False High Sequest HT (A2) Unambiguous AREAAAGIQWSEEETEDEEEEK [K].aREAAAGIQWsEEEtEDEEEEk.[E] N-Term(TMT6plex); S11(Phospho); T15(Phospho); K22(TMT6plex) 4 P46087 P46087-3; P46087-2; P46087; P46087-4 1 3 0.5431 0 1 1 1052.1226 3154.35326 3154.3543 -0.33 -0.00035 908352.4375 CID (Collision Induced Dissociation) MS2 0.0 30 67.8 60.0 83.0769 26319 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 61.7 72.9 56.0 68.2 63.7 72.1 81.7 67.0 72.1 62.7 Excluded by Method 4.29 1 0.0003003 7.653e-05 False High Sequest HT (A2) Unambiguous QREMLMEDVGSEEEQEEEDEAPFQEK [K].qREmLmEDVGsEEEQEEEDEAPFQEk.[D] N-Term(TMT6plex); M4(Oxidation); M6(Oxidation); S11(Phospho); K26(TMT6plex) 2 Q9H1E3 Q9H1E3; Q9H1E3-2 1 4 0.5709 0 1 1 928.65438 3711.5957 3711.59672 -0.27 -0.00025 1574519.0 CID (Collision Induced Dissociation) MS2 4.40994 50 74.7 59.254 84.1033 26710 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 70.3 82.3 62.5 77.4 70.9 63.9 86.5 89.2 64.8 78.9 Excluded by Method 5.43 1 0.0003003 1.89e-05 False High Sequest HT (A2) Unambiguous DTDIGLVNDDANSDEK [K].dTDIGLVNDDANsDEk.[E] N-Term(TMT6plex); S13(Phospho); K16(TMT6plex) 1 Q06106 Q06106 0 3 0.4557 0 1 1 753.68681 2259.04586 2259.04299 1.27 0.00096 2563785.0 CID (Collision Induced Dissociation) MS2 15.87648 50 56.1 29.596 83.9195 26641 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 17.7 45.3 76.6 22.3 50.7 109.0 21.0 47.1 111.5 59.4 Excluded by Method 6.32 1 0.0003003 8.871e-07 False High Sequest HT (A2) Unambiguous KPYRIESDEEEDFENVGK [K].kPYRIEsDEEEDFENVGk.[V] N-Term(TMT6plex); K1(TMT6plex); S7(Phospho); K18(TMT6plex) 3 Q96T23 Q96T23; Q96T23-3; Q96T23-2 1 3 0.1678 0 1 1 984.49291 2951.46416 2951.46426 -0.03 -3e-05 3548901.5 CID (Collision Induced Dissociation) MS2 0.0 20 38.4 33.01 87.2047 27874 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 35.7 33.6 34.1 34.7 38.7 44.2 40.2 44.7 39.4 39.0 Excluded by Method 4.59 1 0.0003003 2.502e-05 False High Sequest HT (A2) Unambiguous QREMLMEDVGSEEEQEEEDEAPFQEK [K].qREMLmEDVGsEEEQEEEDEAPFQEk.[D] N-Term(TMT6plex); M6(Oxidation); S11(Phospho); K26(TMT6plex) 2 Q9H1E3 Q9H1E3; Q9H1E3-2 1 3 0.0085 0 1 1 1232.53719 3695.59703 3695.6018 -1.29 -0.00159 303673.3125 CID (Collision Induced Dissociation) MS2 0.0 30 64.2 60.0 91.4536 29469 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 68.9 67.5 43.2 69.7 63.2 70.9 64.4 68.3 60.7 65.1 Excluded by Method 4.72 1 0.0003003 4.59e-06 False High Sequest HT (A2) Unambiguous IELSDDSDVEVHPNVDK [K].iELSDDsDVEVHPNVDk.[K] N-Term(TMT6plex); S7(Phospho); K17(TMT6plex) 1 P06101 P06101 0 3 0.0616 0 1 1 817.06822 2449.19011 2449.18999 0.05 4e-05 1724758.5 CID (Collision Induced Dissociation) MS2 0.0 20 44.8 60.0 91.3096 29416 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 13.1 34.4 70.3 11.1 34.1 80.2 16.6 42.4 93.5 52.7 Excluded by Method 6.82 1 0.0003003 1.131e-06 False High Sequest HT (A2) Unambiguous DSVDDNENSDDGLDIPK [K].dSVDDNENsDDGLDIPk.[N] N-Term(TMT6plex); S9(Phospho); K17(TMT6plex) 1 Q06631 Q06631 0 3 0.2261 0 1 1 796.02792 2386.06919 2386.06993 -0.31 -0.00025 5403828.5 CID (Collision Induced Dissociation) MS2 4.627239 20 95.4 27.984 91.2663 29398 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 25.0 80.6 124.9 33.8 77.5 177.3 31.6 96.1 204.1 103.3 Excluded by Method 5.13 1 0.0003003 7.159e-06 False High Sequest HT (A2) Unambiguous VVDYSQFQESDDADEDYGRDSGPPTK [K].vVDYSQFQEsDDADEDYGRDSGPPTk.[K] N-Term(TMT6plex); S10(Phospho); K26(TMT6plex) 1 Q9H1E3 Q9H1E3 1 3 0.1385 0 1 1 1153.51536 3458.53154 3458.53134 0.06 7e-05 1147720.125 CID (Collision Induced Dissociation) MS2 0.0 60 23.4 60.0 91.1695 29363 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 23.7 24.6 20.4 23.4 22.1 25.7 23.5 26.7 22.8 21.3 Excluded by Method 5.56 1 0.0003003 9.695e-06 False High Sequest HT (A2) Unambiguous LWNLQNANNK [K].lWNLQNANNk.[S] N-Term(TMT6plex); K10(TMT6plex) 1 P16649 P16649 0 3 0.4526 0 1 1 558.3226 1672.95324 1672.95351 -0.16 -9e-05 2860867.0 CID (Collision Induced Dissociation) MS2 6.83626 70 50.3 53.763 91.5543 29507 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 26.1 44.7 63.6 28.2 39.5 81.9 35.1 51.2 77.4 55.5 3.8 1 0.0003003 0.0001961 False High Sequest HT (A2) Unambiguous GILAADESTGSIAK [K].gILAADESTGsIAk.[R] N-Term(TMT6plex); S11(Phospho); K14(TMT6plex) 2 P04075 P04075; P04075-2 0 3 0.0879 0 1 1 624.33584 1870.99298 1870.99273 0.13 8e-05 1624298.5 CID (Collision Induced Dissociation) MS2 8.865902 40 104.0 60.0 91.0458 29316 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 109.5 118.4 74.8 108.6 95.6 104.9 115.6 110.7 98.2 103.4 Excluded by Method 2.73 1 0.0003003 7.514e-05 False High Sequest HT (A2) Unambiguous SNSLITSTDPVEDHISK [K].sNSLITSTDPVEDHISk.[Y] N-Term(TMT6plex); S1(Phospho); K17(TMT6plex) 1 Q06146 Q06146 0 3 0.0808 0 1 1 794.4056 2381.20226 2381.20016 0.88 0.0007 2154171.5 CID (Collision Induced Dissociation) MS2 6.207566 40 28.7 40.738 90.7455 29201 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 11.2 24.6 39.9 13.8 19.6 52.0 14.0 26.7 54.0 31.4 Excluded by Method 4.33 1 0.0003003 0.0001604 False High Sequest HT (A2) Unambiguous TMFAQVESDDEEAK [K].tMFAQVEsDDEEAk.[N] N-Term(TMT6plex); S8(Phospho); K14(TMT6plex) 1 Q9NW82 Q9NW82 0 3 0.4834 0 1 1 713.33025 2137.9762 2137.97649 -0.14 -0.0001 1430935.875 CID (Collision Induced Dissociation) MS2 50.36958 50 155.2 56.936 91.6997 29562 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 157.0 149.0 115.2 172.4 145.1 142.7 176.5 170.3 165.1 158.2 Excluded by Method 5.73 1 0.0003003 7.399e-08 False High Sequest HT (A2) Unambiguous SLPENSTASAASAFDK [K].sLPENSTASAASAFDk.[A] N-Term(TMT6plex); S1(Phospho); K16(TMT6plex) 1 P41832 P41832 0 3 0.0048 0 1 1 712.02044 2134.04676 2134.04695 -0.09 -6e-05 3539165.75 CID (Collision Induced Dissociation) MS2 14.81987 60 195.2 35.371 93.5734 30259 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 60.5 159.2 302.9 55.9 156.9 380.7 71.8 168.0 393.5 202.5 Excluded by Method 4.13 1 0.0003003 1.501e-05 False High Sequest HT (A2) Unambiguous GEDPYISLPNQEDSQEEK [K].gEDPYISLPNQEDsQEEk.[Q] N-Term(TMT6plex); S14(Phospho); K18(TMT6plex) 1 P21304 P21304 0 3 0.5119 0 1 1 872.74262 2616.21331 2616.21184 0.56 0.00049 806549.4375 CID (Collision Induced Dissociation) MS2 44.42939 60 122.2 60.0 93.4906 30230 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 30.9 106.4 179.5 36.3 100.9 228.3 44.3 120.4 242.0 133.4 Excluded by Method 5.45 1 0.0003003 6.402e-05 False High Sequest HT (A2) Unambiguous LVQIAEEESDK [K].lVQIAEEEsDk.[E] N-Term(TMT6plex); S9(Phospho); K11(TMT6plex) 1 Q06810 Q06810 0 3 0.3753 0 1 1 600.31282 1798.92389 1798.92398 -0.05 -3e-05 1575928.125 CID (Collision Induced Dissociation) MS2 5.563942 90 53.9 60.0 93.485 30228 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 19.7 46.6 70.1 23.0 47.8 83.9 23.5 47.2 117.2 59.7 3.81 1 0.0003003 0.0005592 False High Sequest HT (A2) Unambiguous SYQNSPSSDDGIRPLPEYSTEK [K].sYQNSPssDDGIRPLPEYSTEk.[H] N-Term(TMT6plex); S7(Phospho); S8(Phospho); K22(TMT6plex) 1 Q15648 Q15648 0 3 0.0149 0 1 1 1030.13571 3088.39256 3088.39538 -0.91 -0.00094 714524.4375 CID (Collision Induced Dissociation) MS2 43.94389 40 66.4 60.0 91.916 29643 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 68.4 66.6 61.2 66.3 60.5 75.3 73.4 70.7 57.9 63.9 Excluded by Method 4.69 1 0.0003003 0.000285 False High Sequest HT (A2) Unambiguous KSPSEARQDVDLCLVSSCEYK [K].ksPSEARQDVDLcLVSScEYk.[H] N-Term(TMT6plex); K1(TMT6plex); S2(Phospho); C13(Carbamidomethyl); C18(Carbamidomethyl); K21(TMT6plex) 1 P46821 P46821 2 4 0.0997 0 1 1 810.40734 3238.60755 3238.60923 -0.52 -0.00042 860668.1875 CID (Collision Induced Dissociation) MS2 26.69986 30 91.9 58.987 91.7593 29584 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 65.9 92.8 88.7 80.6 79.9 108.9 81.5 98.3 127.8 94.4 Excluded by Method 3.51 1 0.0003003 0.0003713 False High Sequest HT (A2) Unambiguous EEPLSEEEPCTSTAIASPEK [K].eEPLSEEEPctSTAIAsPEk.[K] N-Term(TMT6plex); C10(Carbamidomethyl); T11(Phospho); S17(Phospho); K20(TMT6plex) 1 Q9Y2X3 Q9Y2X3 0 3 0.0752 0 1 1 941.42151 2822.24998 2822.24963 0.13 0.00012 2384767.75 CID (Collision Induced Dissociation) MS2 0.0 60 94.5 60.0 90.6234 29155 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 84.9 96.5 71.4 104.7 91.3 90.1 115.8 95.5 98.2 96.1 Excluded by Method 4.52 1 0.0003003 3.766e-05 False High Sequest HT (A2) Unambiguous VPSPLEGSEGDGDTD [K].vPSPLEGSEGDGDtD.[-] N-Term(TMT6plex); T14(Phospho) 2 Q9Y606 Q9Y606; Q9Y606-2 0 2 0.2703 0 1 1 892.37704 1783.74681 1783.74725 -0.25 -0.00022 2104978.5 CID (Collision Induced Dissociation) MS2 17.9118 100 52.5 54.84 88.1826 28242 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 44.3 52.3 51.6 49.4 41.4 62.0 55.7 55.9 61.7 50.3 4.92 1 0.0003003 8.477e-07 False High Sequest HT (A2) Unambiguous LFEDDDSNEK [K].lFEDDDsNEk.[L] N-Term(TMT6plex); S7(Phospho); K10(TMT6plex) 1 O43719 O43719 0 2 0.4979 0 1 1 875.40375 1749.80022 1749.79845 1.01 0.00089 1513514.5 CID (Collision Induced Dissociation) MS2 0.0 60 114.1 60.0 88.0012 28174 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 111.0 103.8 86.2 142.2 110.4 110.6 134.2 117.4 122.3 103.2 Excluded by Method 4.74 1 0.0003003 3.744e-05 False High Sequest HT (A2) Unambiguous DTFEHDPSESIDEFNK [K].dTFEHDPSEsIDEFNk.[S] N-Term(TMT6plex); S10(Phospho); K16(TMT6plex) 4 Q9NYF8 Q9NYF8-3; Q9NYF8-4; Q9NYF8; Q9NYF8-2 0 3 0.1777 0 1 1 816.70579 2448.10283 2448.10084 0.81 0.00066 7773279.5 CID (Collision Induced Dissociation) MS2 3.235728 50 123.7 18.786 87.9899 28169 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 119.0 134.6 98.1 136.3 114.5 118.7 146.3 122.6 120.3 126.6 Excluded by Method 6.98 1 0.0003003 2.543e-06 False High Sequest HT (A2) Unambiguous EEPLSEEEPCTSTAIASPEK [K].eEPLsEEEPcTSTAIASPEk.[K] N-Term(TMT6plex); S5(Phospho); C10(Carbamidomethyl); K20(TMT6plex) 1 Q9Y2X3 Q9Y2X3 0 3 0.2478 0 1 1 914.76586 2742.28302 2742.28329 -0.1 -9e-05 23700148.0 CID (Collision Induced Dissociation) MS2 0.0 70 115.2 6.268 87.7594 28084 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 117.6 121.0 90.1 114.3 106.7 115.1 120.5 121.0 117.7 128.4 4.56 1 0.0003003 0.0004605 False High Sequest HT (A2) Unambiguous VFDDESDEKEDEEYADEK [K].vFDDEsDEkEDEEYADEk.[G] N-Term(TMT6plex); S6(Phospho); K9(TMT6plex); K18(TMT6plex) 1 O43719 O43719 1 3 0.1615 0 1 1 987.1121 2959.32175 2959.32247 -0.24 -0.00024 1657704.25 CID (Collision Induced Dissociation) MS2 10.59609 30 62.0 60.0 87.6736 28054 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 60.7 52.0 44.5 82.9 57.7 57.4 75.9 66.9 65.4 57.0 Excluded by Method 5.51 1 0.0003003 3.906e-05 False High Sequest HT (A2) Unambiguous SIFDTDDDD [K].sIFDtDDDD.[-] N-Term(TMT6plex); T5(Phospho) 1 P36054 P36054 0 2 0.5046 0 1 1 676.26068 1351.51408 1351.514 0.05 4e-05 1665401.75 CID (Collision Induced Dissociation) MS2 10.70389 70 118.0 60.0 90.2759 29026 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 32.5 91.7 189.6 34.3 96.5 222.3 46.1 112.0 240.7 114.6 3.27 1 0.0003003 0.0001466 False High Sequest HT (A2) Unambiguous ALSYASLDK [K].aLSYAsLDk.[E] N-Term(TMT6plex); S6(Phospho); K9(TMT6plex) 2 Q6R327 Q6R327; Q6R327-3 0 2 0.0556 0 1 1 753.40433 1505.80139 1505.80168 -0.19 -0.00014 1616037.75 CID (Collision Induced Dissociation) MS2 20.51694 30 118.3 60.0 90.1712 28985 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 126.4 112.1 93.2 140.1 106.8 107.2 139.7 119.6 110.5 127.4 Excluded by Method 3.42 1 0.0003003 0.0005366 False High Sequest HT (A2) Unambiguous IGEGTYGVVYK [K].iGEGTyGVVYk.[AG] N-Term(TMT6plex); Y6(Phospho); K11(TMT6plex) 5 P24941 P24941; P06493; Q00526; P24941-2; P06493-2 0 2 0.0134 0 1 1 862.45791 1723.90854 1723.90721 0.77 0.00067 38409068.0 CID (Collision Induced Dissociation) MS2 8.683517 80 62.1 11.972 89.9748 28909 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 61.5 53.1 51.8 65.1 58.3 64.6 68.3 67.4 64.1 66.3 4.49 1 0.0003003 3.882e-06 False High Sequest HT (A2) Unambiguous APVPSTCSSTFPEELSPPSHQAK [K].aPVPSTcSSTFPEELsPPSHQAk.[R] N-Term(TMT6plex); C7(Carbamidomethyl); S16(Phospho); K23(TMT6plex) 5 Q14980 Q14980-3; Q14980-5; Q14980-2; Q14980-4; Q14980 0 3 0.1178 0 1 1 998.15523 2992.45115 2992.45276 -0.54 -0.00054 314212.5 CID (Collision Induced Dissociation) MS2 0.0 40 14.2 60.0 89.5165 28741 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 13.5 18.7 9.2 15.8 14.5 13.7 15.4 14.7 11.6 15.3 Excluded by Method 5.52 1 0.0003003 3.832e-05 False High Sequest HT (A2) Unambiguous VVDYSQFQESDDADEDYGRDSGPPTK [K].vVDYSQFQEsDDADEDYGRDSGPPTk.[K] N-Term(TMT6plex); S10(Phospho); K26(TMT6plex) 1 Q9H1E3 Q9H1E3 1 4 0.1536 0 1 1 865.38716 3458.52683 3458.53134 -1.3 -0.00113 363065.0625 CID (Collision Induced Dissociation) MS2 11.26975 60 28.1 60.0 89.3336 28673 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 23.4 33.6 24.5 25.5 27.8 33.0 26.7 28.9 32.6 24.9 Excluded by Method 6.9 1 0.0003003 1.61e-06 False High Sequest HT (A2) Unambiguous SVDELITEGNEK [K].sVDELITEGNEk.[L] N-Term(TMT6plex); K12(TMT6plex) 1 P05319 P05319 0 3 0.1945 0 1 1 597.9958 1791.97286 1791.97403 -0.65 -0.00039 489058.59375 CID (Collision Induced Dissociation) MS2 58.55117 40 40.4 60.0 89.123 28594 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 34.4 36.3 47.3 26.8 35.4 51.8 32.6 41.0 57.6 40.6 Excluded by Method 2.93 1 0.0003003 0.0002002 False High Sequest HT (A2) Unambiguous GLQTVVVEEDA [K].gLQTVVVEEDA.[-] N-Term(TMT6plex) 2 P0C2H8 P0C2H8; P0C2H9 0 2 0.6773 0 1 1 694.87698 1388.74667 1388.74704 -0.26 -0.00018 2832655.5 CID (Collision Induced Dissociation) MS2 6.802808 90 262.2 60.0 89.0137 28552 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 61.4 207.9 448.2 76.0 200.4 505.2 88.3 236.1 541.5 256.5 2.82 1 0.0003003 0.0003756 False High Sequest HT (A2) Unambiguous LFDEEEDSSEK [K].lFDEEEDSsEk.[L] N-Term(TMT6plex); S9(Phospho); K11(TMT6plex) 1 O43719 O43719 0 3 0.1268 0 1 1 622.6199 1865.84516 1865.84579 -0.34 -0.00021 1056974.875 CID (Collision Induced Dissociation) MS2 13.62234 80 159.6 60.0 88.909 28513 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 158.9 156.7 129.1 192.2 159.5 142.6 187.3 167.3 155.9 146.9 4.26 1 0.0003003 5.912e-05 False High Sequest HT (A2) Unambiguous YDEYFNEYNNNSN [K].yDEYFNEYNNNSN.[-] N-Term(TMT6plex) 1 P32776 P32776 0 2 0.6886 0 1 1 957.90335 1914.79943 1914.79797 0.76 0.00073 618129.0625 CID (Collision Induced Dissociation) MS2 16.05089 100 95.7 60.0 88.8935 28507 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 22.7 76.6 154.5 26.5 74.2 186.7 32.3 98.1 187.7 97.6 3.95 1 0.0003003 6.481e-06 False High Sequest HT (A2) Unambiguous ATLEEAEGESGVEDDAATGSSNK [K].aTLEEAEGEsGVEDDAATGsSNk.[L] N-Term(TMT6plex); S10(Phospho); S20(Phospho); K23(TMT6plex) 1 P21304 P21304 0 3 0.0609 0 1 1 962.41738 2885.2376 2885.23787 -0.1 -9e-05 1110657.125 CID (Collision Induced Dissociation) MS2 7.228503 80 39.2 60.0 81.6457 25780 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 9.5 27.8 60.7 15.4 33.9 71.6 15.8 36.9 74.6 45.6 5.42 1 0.0003003 4.626e-06 False High Sequest HT (A2) Unambiguous RLLGDSDSEEEQK [K].rLLGDsDsEEEQk.[E] N-Term(TMT6plex); S6(Phospho); S8(Phospho); K13(TMT6plex) 1 Q9BW71 Q9BW71 1 3 0.6048 0 1 1 708.65997 2123.96536 2123.96633 -0.46 -0.00032 2075057.625 CID (Collision Induced Dissociation) MS2 4.168904 50 147.8 57.378 73.2488 22619 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 146.0 138.2 118.6 167.0 150.5 140.6 170.4 152.4 146.3 148.0 Excluded by Method 5.39 1 0.0003003 7.71e-05 False High Sequest HT (A2) Unambiguous DYNASASTISPPSSMEEDK [K].dYNASASTIsPPSSmEEDk.[F] N-Term(TMT6plex); S10(Phospho); M15(Oxidation); K19(TMT6plex) 1 P46821 P46821 0 3 0.1975 0 1 1 861.72341 2583.15569 2583.15737 -0.65 -0.00056 571527.625 CID (Collision Induced Dissociation) MS2 71.48392 30 45.8 60.0 72.5083 22343 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 46.6 50.6 40.6 44.8 45.8 42.2 53.5 45.6 41.2 47.6 Excluded by Method 4.71 1 0.0003003 3.187e-05 False High Sequest HT (A2) Unambiguous SSTSFANIQENSN [K].sSTsFANIQENSN.[-] N-Term(TMT6plex); S4(Phospho) 1 Q86WC4 Q86WC4 0 2 0.0289 0 1 1 854.37424 1707.74121 1707.74244 -0.72 -0.00061 7258175.5 CID (Collision Induced Dissociation) MS2 4.321206 70 166.8 54.356 72.4192 22310 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 170.3 157.4 146.6 156.9 152.9 168.4 185.7 182.2 176.2 171.5 4.15 1 0.0003003 1.111e-06 False High Sequest HT (A2) Unambiguous TVPQISDQETEK [K].tVPQIsDQETEk.[R] N-Term(TMT6plex); S6(Phospho); K12(TMT6plex) 23 P0C2I9 P0C2I9; P47100; Q12141; P47098; P0C2J0; P0C2I3; Q12269; Q03855; P0C2I6; P0C2I2; Q99231; Q12414; Q07793; Q04711; Q04670; Q12273; Q03612; Q92393; P0C2I5; Q03434; O13527; P0C2J1; P0C2I7 0 3 0.2016 0 1 1 638.32703 1912.96653 1912.96691 -0.2 -0.00013 2084779.125 CID (Collision Induced Dissociation) MS2 8.440408 90 151.8 60.0 72.9138 22496 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 41.7 123.0 249.6 49.9 116.2 284.3 59.4 138.3 306.4 149.6 4.96 1 0.0003003 1.517e-05 False High Sequest HT (A2) Unambiguous TMFAQVESDDEEAK [K].tmFAQVEsDDEEAk.[N] N-Term(TMT6plex); M2(Oxidation); S8(Phospho); K14(TMT6plex) 1 Q9NW82 Q9NW82 0 3 0.4253 0 1 1 718.66184 2153.97098 2153.9714 -0.2 -0.00014 8443252.0 CID (Collision Induced Dissociation) MS2 1.933316 50 112.5 25.748 74.6184 23137 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 119.0 114.4 89.0 125.4 107.3 111.4 116.0 122.9 117.0 102.7 Excluded by Method 5.29 1 0.0003003 9.093e-07 False High Sequest HT (A2) Unambiguous FTPPAESGSPSK [K].fTPPAESGsPSk.[E] N-Term(TMT6plex); S9(Phospho); K12(TMT6plex) 1 Q70E73 Q70E73 0 3 0.2272 0 1 1 581.63011 1742.87578 1742.87664 -0.49 -0.00029 2134815.75 CID (Collision Induced Dissociation) MS2 10.2035 50 117.4 43.375 73.9145 22870 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 120.4 119.3 94.6 120.0 113.6 112.3 133.5 123.8 121.0 115.1 Excluded by Method 5.59 1 0.0003003 2.147e-05 False High Sequest HT (A2) Unambiguous AAAYDISEDEED [K].aAAYDIsEDEED.[-] N-Term(TMT6plex); S7(Phospho) 1 P49585 P49585 0 2 0.2448 0 1 1 818.82664 1636.646 1636.64647 -0.29 -0.00023 795829.375 CID (Collision Induced Dissociation) MS2 23.31121 60 246.5 60.0 74.2194 22986 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 275.9 246.3 224.0 238.4 233.1 244.5 262.2 253.6 243.3 243.7 Excluded by Method 3.84 1 0.0003003 1.906e-05 False High Sequest HT (A2) Unambiguous DETFGEYSDNEEK [K].dETFGEYsDNEEk.[A] N-Term(TMT6plex); S8(Phospho); K13(TMT6plex) 2 P32004-2 P32004-3; P32004-2 0 2 0.0543 0 1 1 1050.95525 2100.90322 2100.9051 -0.89 -0.00094 1623314.625 CID (Collision Induced Dissociation) MS2 0.0 30 134.4 60.0 74.1921 22975 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 131.0 130.6 98.6 147.6 129.4 118.2 176.1 140.2 134.2 137.9 Excluded by Method 5.34 1 0.0003003 7.36e-06 False High Sequest HT (A2) Unambiguous SNFSNSADDIK [K].sNFSNSADDIk.[S] N-Term(TMT6plex); S1(Phospho); K11(TMT6plex) 1 P45973 P45973 0 3 0.0228 0 1 1 579.28136 1735.82953 1735.83042 -0.51 -0.0003 790356.9375 CID (Collision Induced Dissociation) MS2 48.78358 80 57.8 60.0 74.1064 22942 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 65.0 53.9 44.0 67.5 56.1 54.9 58.4 65.1 57.4 55.6 3.95 1 0.0003003 3.556e-05 False High Sequest HT (A2) Unambiguous AGFNYESPSK [K].aGFNYEsPSk.[G] N-Term(TMT6plex); S7(Phospho); K10(TMT6plex) 3 Q9UHF7 Q9UHF7-3; Q9UHF7-2; Q9UHF7 0 3 0.3231 0 1 1 546.60405 1637.7976 1637.79766 -0.04 -2e-05 2060901.0 CID (Collision Induced Dissociation) MS2 3.174201 60 125.4 60.0 74.3303 23030 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 130.3 119.7 100.2 134.6 112.1 118.2 137.5 149.7 125.4 126.5 Excluded by Method 4.24 1 0.0003003 0.0001952 False High Sequest HT (A2) Unambiguous AESSDSGAESEEEEAQEEVK [K].aESSDsGAEsEEEEAQEEVk.[G] N-Term(TMT6plex); S6(Phospho); S10(Phospho); K20(TMT6plex) 4 Q5T8D3 Q5T8D3-4; Q5T8D3-3; Q5T8D3-2; Q5T8D3 0 3 0.0722 0 1 1 920.04863 2758.13132 2758.12693 1.59 0.00147 412294.3125 CID (Collision Induced Dissociation) MS2 25.74598 70 16.2 60.0 75.1435 23334 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 15.7 15.7 13.6 14.5 13.6 18.8 17.0 18.6 19.9 14.2 3.88 1 0.0003003 0.0001704 False High Sequest HT (A2) Unambiguous EVEDKESEGEEEDEDEDLSK [K].eVEDkEsEGEEEDEDEDLSk.[Y] N-Term(TMT6plex); K5(TMT6plex); S7(Phospho); K20(TMT6plex) 2 O95218 O95218-2; O95218 1 3 0.4939 0 1 1 1036.46846 3107.39082 3107.392 -0.38 -0.00039 828652.4375 CID (Collision Induced Dissociation) MS2 0.0 20 41.6 60.0 70.5572 21613 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 51.0 34.7 25.0 49.1 42.2 40.2 52.2 43.5 41.7 36.7 Excluded by Method 4.11 1 0.0003003 0.0002177 False High Sequest HT (A2) Unambiguous EDSAPVAAGESESSL [K].eDSAPVAAGESEsSL.[-] N-Term(TMT6plex); S13(Phospho) 1 P25567 P25567 0 2 0.0022 0 1 1 879.38671 1757.76615 1757.76799 -1.05 -0.00092 5507869.0 CID (Collision Induced Dissociation) MS2 0.0 80 94.7 43.865 71.9975 22152 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 26.7 76.0 157.4 26.3 74.8 186.1 29.1 87.0 184.8 98.3 4.5 1 0.0003003 5.758e-05 False High Sequest HT (A2) Unambiguous RLLGDSDSEEEQK [K].rLLGDsDsEEEQk.[E] N-Term(TMT6plex); S6(Phospho); S8(Phospho); K13(TMT6plex) 1 Q9BW71 Q9BW71 1 3 0.617 0 1 1 708.66015 2123.96591 2123.96633 -0.2 -0.00014 3639937.0 CID (Collision Induced Dissociation) MS2 6.362003 40 144.3 56.1 71.6837 22032 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 138.9 153.5 108.3 160.8 139.7 131.2 180.8 151.0 144.0 134.9 Excluded by Method 5.64 1 0.0003003 1.542e-05 False High Sequest HT (A2) Unambiguous KLNELQNSEDSDAEDGGK [K].kLNELQNSEDsDAEDGGk.[Q] N-Term(TMT6plex); K1(TMT6plex); S11(Phospho); K18(TMT6plex) 1 Q12136 Q12136 1 3 0.1763 0 1 1 906.11384 2716.32695 2716.32816 -0.44 -0.0004 292011.59375 CID (Collision Induced Dissociation) MS2 0.0 90 20.8 60.0 71.0567 21802 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 3.6 16.7 27.6 10.9 17.6 34.6 12.2 18.2 46.5 20.2 4.48 1 0.0003003 4.228e-05 False High Sequest HT (A2) Unambiguous TVSPTTQISAGK [K].tVSPtTQISAGk.[S] N-Term(TMT6plex); T5(Phospho); K12(TMT6plex) 1 P53297 P53297 0 3 0.0093 0 1 1 576.64996 1727.93531 1727.93449 0.48 0.00028 673458.375 CID (Collision Induced Dissociation) MS2 5.832373 80 122.1 60.0 70.991 21777 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 31.0 91.5 183.9 38.8 100.4 246.7 44.8 112.8 237.1 134.0 3.23 1 0.0003003 0.0002247 False High Sequest HT (A2) Unambiguous ENETDEENTEVMIK [K].eNEtDEENTEVmIk.[G] N-Term(TMT6plex); T4(Phospho); M12(Oxidation); K14(TMT6plex) 1 Q9HAU5 Q9HAU5 0 3 0.2306 0 1 1 745.67589 2235.01313 2235.014 -0.39 -0.00029 964752.375 CID (Collision Induced Dissociation) MS2 11.91817 60 129.4 60.0 70.8064 21706 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 128.7 114.4 109.6 142.3 130.3 127.7 153.2 128.3 134.6 124.7 Excluded by Method 5.42 1 0.0003003 4.787e-05 False High Sequest HT (A2) Unambiguous RTCPSLSPTSPLNNK [K].rTcPSLSPTsPLNNk.[G] N-Term(TMT6plex); C3(Carbamidomethyl); S10(Phospho); K15(TMT6plex) 2 O75925 O75925; O75925-2 1 3 0.1152 0 1 1 737.38508 2210.14068 2210.14047 0.09 7e-05 716711.875 CID (Collision Induced Dissociation) MS2 9.82812 40 115.4 60.0 70.9463 21760 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 129.6 112.4 99.1 114.6 110.3 111.0 127.9 124.5 110.4 114.5 Excluded by Method 4.34 1 0.0003003 0.000527 False High Sequest HT (A2) Unambiguous VFDDESDEK [K].vFDDEsDEk.[E] N-Term(TMT6plex); S6(Phospho); K9(TMT6plex) 1 O43719 O43719 0 2 0.2284 0 1 1 811.37267 1621.73807 1621.73987 -1.11 -0.0009 1438757.0 CID (Collision Induced Dissociation) MS2 0.0 30 269.7 60.0 75.2814 23387 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 263.7 257.9 211.0 310.2 259.2 241.2 352.8 285.3 267.6 247.8 Excluded by Method 4.16 1 0.0003003 0.000126 False High Sequest HT (A2) Unambiguous DDFESEEEDVK [K].dDFEsEEEDVk.[S] N-Term(TMT6plex); S5(Phospho); K11(TMT6plex) 3 Q7Z6E9 Q7Z6E9-4; Q7Z6E9-2; Q7Z6E9 0 2 0.5808 0 1 1 940.41584 1879.8244 1879.82506 -0.35 -0.00033 510553.71875 CID (Collision Induced Dissociation) MS2 0.0 40 81.8 60.0 79.8279 25098 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 79.8 87.3 53.5 106.0 77.1 69.4 89.3 80.7 84.5 90.0 Excluded by Method 5.2 1 0.0003003 1.762e-05 False High Sequest HT (A2) Unambiguous DSDGVQVANESEEDPELK [K].dSDGVQVANEsEEDPELk.[E] N-Term(TMT6plex); S11(Phospho); K18(TMT6plex) 1 Q07418 Q07418 0 3 0.4782 0 1 1 833.72298 2499.15437 2499.15399 0.15 0.00013 1059637.375 CID (Collision Induced Dissociation) MS2 5.888286 70 38.1 60.0 79.1409 24843 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 19.4 30.7 45.2 23.8 33.6 62.9 23.2 37.2 64.1 40.9 4.35 1 0.0003003 3.581e-05 False High Sequest HT (A2) Unambiguous QETVADFTPK [K].qETVADFtPk.[K] N-Term(TMT6plex); T8(Phospho); K10(TMT6plex) 3 O75400 O75400-2; O75400-3; O75400 0 3 0.488 0 1 1 558.62344 1673.85576 1673.85517 0.35 0.0002 4475888.0 CID (Collision Induced Dissociation) MS2 3.259221 70 126.1 29.881 78.7582 24695 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 134.8 129.2 101.2 130.9 114.8 114.4 134.8 131.0 147.0 122.6 5.0 1 0.0003003 3.996e-06 False High Sequest HT (A2) Unambiguous ATVNARPQRVLDTSSLTQSAPASPTNK [K].aTVNARPQRVLDTSSLTQsAPAsPTNk.[G] N-Term(TMT6plex); S19(Phospho); S23(Phospho); K27(TMT6plex) 2 Q8N122 Q8N122-3; Q8N122 1 4 0.2486 0 1 1 857.93936 3428.73562 3428.73403 0.46 0.0004 2635732.25 CID (Collision Induced Dissociation) MS2 0.0 30 33.8 49.333 78.7424 24688 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 36.1 39.1 25.6 31.1 29.0 36.8 42.4 27.5 32.8 37.8 Excluded by Method 3.62 1 0.0003003 0.0001037 False High Sequest HT (A2) Unambiguous VEMYSGSDDDDDFNK [K].vEmYSGsDDDDDFNk.[L] N-Term(TMT6plex); M3(Oxidation); S7(Phospho); K15(TMT6plex) 1 O60841 O60841 0 3 0.0805 0 1 1 764.32027 2290.94626 2290.94631 -0.02 -2e-05 488518.40625 CID (Collision Induced Dissociation) MS2 0.0 40 42.9 60.0 78.6668 24660 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 40.2 41.5 38.7 46.6 43.2 45.0 48.1 46.2 43.9 35.5 Excluded by Method 4.35 1 0.0003003 9.377e-06 False High Sequest HT (A2) Unambiguous QSFDDNDSEELEDK [K].qSFDDNDsEELEDk.[D] N-Term(TMT6plex); S8(Phospho); K14(TMT6plex) 1 O60841 O60841 0 3 0.1343 0 1 1 736.99116 2208.95892 2208.95859 0.15 0.00011 6403438.0 CID (Collision Induced Dissociation) MS2 0.8918251 20 121.4 22.422 78.4198 24567 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 126.6 127.9 97.7 128.6 110.1 105.2 129.4 139.3 134.3 114.4 Excluded by Method 4.32 1 0.0003003 9.921e-05 False High Sequest HT (A2) Unambiguous SSSPELVTHLK [K].sSsPELVTHLk.[W] N-Term(TMT6plex); S3(Phospho); K11(TMT6plex) 1 Q07960 Q07960 0 3 0.0231 0 1 1 579.31804 1735.93957 1735.93957 0.0 0.0 1289680.0 CID (Collision Induced Dissociation) MS2 16.11286 90 132.3 60.0 78.2805 24517 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 135.7 130.7 112.5 140.9 123.4 134.3 149.1 138.9 125.8 132.1 4.33 1 0.0003003 0.0002788 False High Sequest HT (A2) Unambiguous ATLEEAEGESGVEDDAATGSSNK [K].aTLEEAEGESGVEDDAATGSsNk.[L] N-Term(TMT6plex); S21(Phospho); K23(TMT6plex) 1 P21304 P21304 0 3 0.0604 0 1 1 935.7632 2805.27504 2805.27154 1.24 0.00116 342274.28125 CID (Collision Induced Dissociation) MS2 0.0 90 22.5 60.0 79.9879 25157 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 7.4 17.0 31.3 9.3 20.4 41.4 12.3 19.0 41.3 25.2 2.65 1 0.0003003 0.0005311 False High Sequest HT (A2) Unambiguous ADTTTPTTSAITASRSESPPPLSDPK [K].aDTTTPTTSAITASRSEsPPPLSDPk.[Q] N-Term(TMT6plex); S18(Phospho); K26(TMT6plex) 2 Q15059 Q15059; Q15059-2 1 4 0.0799 0 1 1 792.40329 3166.59132 3166.59209 -0.24 -0.00019 1221432.375 CID (Collision Induced Dissociation) MS2 3.713154 30 110.7 60.0 81.3579 25672 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 112.7 112.5 94.9 108.9 103.5 103.9 125.3 122.9 109.9 112.0 Excluded by Method 4.88 1 0.0003003 6.699e-05 False High Sequest HT (A2) Unambiguous EAPAEGEAAEPGSPTAAEGEAASAASSTSSPK [K].eAPAEGEAAEPGSPtAAEGEAASAASStSSPk.[A] N-Term(TMT6plex); T15(Phospho); T28(Phospho); K32(TMT6plex) 1 P29966 P29966 0 4 0.021 0 1 1 884.14464 3533.55672 3533.561 -1.21 -0.00107 1395642.875 CID (Collision Induced Dissociation) MS2 10.95332 60 85.5 54.411 81.3363 25662 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 104.8 77.3 67.4 93.7 80.8 74.8 104.2 86.0 79.9 85.8 Excluded by Method 5.71 1 0.0003003 2.561e-05 False High Sequest HT (A2) Unambiguous DASQEESLI [K].dASQEEsLI.[-] N-Term(TMT6plex); S7(Phospho) 1 P16140 P16140 0 2 0.4779 0 1 1 650.79734 1300.58741 1300.58711 0.23 0.00015 3273505.5 CID (Collision Induced Dissociation) MS2 2.711201 30 245.3 60.0 81.0694 25559 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 63.3 190.2 414.8 71.5 209.5 441.1 86.0 221.5 496.4 258.9 Excluded by Method 2.49 1 0.0003003 0.0004465 False High Sequest HT (A2) Unambiguous SPAVATSTAAPPPPSSPLPSK [K].sPAVATSTAAPPPPSSPLPsk.[S] N-Term(TMT6plex); S20(Phospho); K21(TMT6plex) 5 Q14157-1 Q14157-1; Q14157-5; Q14157-4; Q14157; Q14157-3 0 3 0.0175 0 1 1 833.44887 2498.33205 2498.33078 0.51 0.00042 6641459.5 CID (Collision Induced Dissociation) MS2 2.488777 30 127.8 22.855 81.0602 25555 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 116.6 139.3 104.6 122.5 125.2 128.7 151.1 141.7 130.2 117.8 Excluded by Method 3.99 1 0.0003003 8.367e-05 False High Sequest HT (A2) Unambiguous FSVSSNDSQNSLK [K].fSVSSNDSQNsLk.[N] N-Term(TMT6plex); S11(Phospho); K13(TMT6plex) 1 Q06833 Q06833 0 3 0.2994 0 1 1 650.99101 1950.95847 1950.95741 0.54 0.00035 1106105.5 CID (Collision Induced Dissociation) MS2 4.397301 40 41.3 60.0 80.5679 25372 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 9.2 27.5 58.2 14.0 35.8 74.3 17.1 41.7 81.1 53.9 Excluded by Method 4.91 1 0.0003003 4.857e-05 False High Sequest HT (A2) Unambiguous EAPAEGEAAEPGSPTAAEGEAASAASSTSSPK [K].eAPAEGEAAEPGsPTAAEGEAASAASSTSSPk.[A] N-Term(TMT6plex); S13(Phospho); K32(TMT6plex) 1 P29966 P29966 0 4 0.0849 0 1 1 864.15468 3453.5969 3453.59467 0.65 0.00056 727475.4375 CID (Collision Induced Dissociation) MS2 29.13174 50 80.6 60.0 80.3432 25288 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 87.6 83.2 68.6 83.6 73.4 79.6 89.0 88.3 79.8 73.1 Excluded by Method 5.18 1 0.0003003 0.0001885 False High Sequest HT (A2) Unambiguous QSFDDNDSEELEDK [K].qSFDDNDsEELEDk.[D] N-Term(TMT6plex); S8(Phospho); K14(TMT6plex) 1 O60841 O60841 0 3 0.163 0 1 1 736.99091 2208.95818 2208.95859 -0.18 -0.00013 507922.71875 CID (Collision Induced Dissociation) MS2 40.62114 50 38.4 60.0 80.2678 25259 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 32.4 47.0 30.7 44.6 33.3 37.8 38.9 39.9 41.6 37.6 Excluded by Method 3.68 1 0.0003003 0.0001496 False High Sequest HT (A2) Unambiguous GEADDEDDDEDGQDNQGTVTEGSSPAYLK [K].gEADDEDDDEDGQDNQGTVTEGsSPAYLk.[E] N-Term(TMT6plex); S23(Phospho); K29(TMT6plex) 9 Q86U86 Q86U86-5; Q86U86-7; Q86U86-4; Q86U86; Q86U86-6; Q86U86-9; Q86U86-8; Q86U86-2; Q86U86-3 0 4 0.0067 0 1 1 899.63313 3595.51071 3595.51212 -0.39 -0.00035 803162.5625 CID (Collision Induced Dissociation) MS2 0.0 40 6.8 60.0 78.2224 24495 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 3.6 8.9 6.6 3.0 3.6 8.3 10.3 6.7 9.7 7.3 Excluded by Method 4.45 1 0.0003003 0.0002159 False High Sequest HT (A2) Unambiguous EQAEASIDNLK [K].eQAEAsIDNLk.[N] N-Term(TMT6plex); S6(Phospho); K11(TMT6plex) 1 Q08245 Q08245 0 2 0.3571 0 1 1 878.44976 1755.89225 1755.89302 -0.44 -0.00038 17897170.0 CID (Collision Induced Dissociation) MS2 1.965186 40 117.4 56.683 76.0736 23686 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 32.6 92.9 179.1 34.1 95.3 213.0 42.0 112.9 242.1 130.3 Excluded by Method 3.36 1 0.0003003 7.225e-05 False High Sequest HT (A2) Unambiguous ELSPAGSISK [K].eLsPAGSISk.[N] N-Term(TMT6plex); S3(Phospho); K10(TMT6plex) 4 O95453 O95453-4; O95453-2; O95453-3; O95453 0 2 0.314 0 1 1 763.91536 1526.82345 1526.82315 0.2 0.00015 559040.1875 CID (Collision Induced Dissociation) MS2 42.95842 40 272.5 60.0 75.8259 23594 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 296.7 271.5 241.0 279.2 258.1 269.8 288.7 300.7 258.3 261.4 Excluded by Method 2.58 1 0.0003003 0.0003299 False High Sequest HT (A2) Unambiguous GSEGYLAATYPTVGQTSPRARK [K].gSEGYLAATYPTVGQTsPRARk.[S] N-Term(TMT6plex); S17(Phospho); K22(TMT6plex) 3 P21359 P21359-2; P21359-6; P21359 2 4 0.0132 0 1 1 712.87487 2848.47764 2848.47588 0.62 0.00044 1612208.375 CID (Collision Induced Dissociation) MS2 0.0 50 60.5 60.0 75.5641 23495 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 61.2 64.5 51.9 50.1 64.6 69.6 70.1 61.2 54.4 57.1 Excluded by Method 4.53 1 0.0003003 2.966e-05 False High Sequest HT (A2) Unambiguous EAPAEGEAAEPGSPTAAEGEAASAASSTSSPK [K].eAPAEGEAAEPGSPTAAEGEAASAASSTSsPk.[A] N-Term(TMT6plex); S30(Phospho); K32(TMT6plex) 1 P29966 P29966 0 3 0.0173 0 1 1 1151.86986 3453.59503 3453.59467 0.1 0.00012 705021.0625 CID (Collision Induced Dissociation) MS2 45.18229 70 62.2 60.0 75.5037 23472 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 65.0 61.6 51.0 76.2 56.9 59.0 71.8 64.9 57.3 58.2 4.62 1 0.0003003 9.521e-07 False High Sequest HT (A2) Unambiguous KLNELQNSEDSDAEDGGK [K].kLNELQNsEDsDAEDGGk.[Q] N-Term(TMT6plex); K1(TMT6plex); S8(Phospho); S11(Phospho); K18(TMT6plex) 1 Q12136 Q12136 1 3 0.5443 0 1 1 932.77339 2796.30563 2796.29449 3.98 0.00371 863637.75 CID (Collision Induced Dissociation) MS2 41.34474 70 82.2 60.0 75.4763 23462 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 34.0 64.6 111.6 35.9 64.5 150.8 33.5 80.9 164.0 82.2 5.53 1 0.0003003 2.662e-05 False High Sequest HT (A2) Unambiguous EDSAPVAAGESESSL [K].eDSAPVAAGEsESSL.[-] N-Term(TMT6plex); S11(Phospho) 1 P25567 P25567 0 2 0.0353 0 1 1 879.38512 1757.76297 1757.76799 -2.85 -0.00251 412683.40625 CID (Collision Induced Dissociation) MS2 0.0 60 66.5 60.0 75.6982 23546 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 15.5 55.0 102.4 20.4 62.2 123.3 22.8 65.8 132.4 65.3 Excluded by Method 3.97 1 0.0003003 0.0004537 False High Sequest HT (A2) Unambiguous AAQQDSSEDENIGGEYYTK [K].aAQQDsSEDENIGGEYYTk.[L] N-Term(TMT6plex); S6(Phospho); K19(TMT6plex) 1 P25037 P25037 0 3 0.0068 0 1 1 881.73363 2643.18634 2643.18636 -0.01 -1e-05 711849.25 CID (Collision Induced Dissociation) MS2 18.58822 70 47.7 60.0 77.8096 24341 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 12.2 40.7 73.1 12.0 41.4 87.8 18.3 51.3 86.2 53.9 4.41 1 0.0003003 1.377e-05 False High Sequest HT (A2) Unambiguous KDESDSENDIEIK [K].kDEsDsENDIEIk.[G] N-Term(TMT6plex); K1(TMT6plex); S4(Phospho); S6(Phospho); K13(TMT6plex) 1 P32499 P32499 1 3 0.4735 0 1 1 790.37625 2369.11421 2369.11295 0.53 0.00042 2023637.375 CID (Collision Induced Dissociation) MS2 13.86564 50 213.1 60.0 77.681 24292 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 67.6 182.6 283.5 101.5 192.5 343.3 109.6 189.4 439.0 221.9 Excluded by Method 6.8 1 0.0003003 9.962e-06 False High Sequest HT (A2) Unambiguous NAVSTKPTPPPAPEASAESGLSSK [K].nAVSTkPTPPPAPEASAESGLSsk.[V] N-Term(TMT6plex); K6(TMT6plex); S23(Phospho); K24(TMT6plex) 1 P16862 P16862 0 3 0.015 0 1 1 1030.88338 3090.6356 3090.63272 0.93 0.00096 624559.375 CID (Collision Induced Dissociation) MS2 0.0 80 75.6 60.0 77.403 24191 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 18.5 61.8 109.7 25.4 60.9 138.6 30.2 68.5 161.8 80.3 7.32 1 0.0003003 2.6e-08 False High Sequest HT (A2) Unambiguous LDEDEDEDDADLSK [K].lDEDEDEDDADLsk.[Y] N-Term(TMT6plex); S13(Phospho); K14(TMT6plex) 2 O95218 O95218-2; O95218 0 2 0.6617 0 1 1 1073.96985 2146.93243 2146.93171 0.34 0.00036 681313.375 CID (Collision Induced Dissociation) MS2 0.0 40 98.1 60.0 77.1965 24113 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 98.7 90.5 70.6 116.6 89.2 85.9 105.1 101.7 115.7 107.4 Excluded by Method 4.73 1 0.0003003 0.0003921 False High Sequest HT (A2) Unambiguous DSNELSDSAGEEDSADLK [K].dSNELsDsAGEEDSADLk.[R] N-Term(TMT6plex); S6(Phospho); S8(Phospho); K18(TMT6plex) 2 Q9Y6X9 Q9Y6X9-2; Q9Y6X9 0 3 0.1184 0 1 1 834.01985 2500.045 2500.04174 1.3 0.00109 581974.75 CID (Collision Induced Dissociation) MS2 20.72993 30 28.5 60.0 77.099 24075 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 25.7 25.8 23.9 25.5 29.2 32.6 30.0 28.5 30.3 33.9 Excluded by Method 4.14 1 0.0003003 3.794e-05 False High Sequest HT (A2) Unambiguous YGNLNDSASNIGK [K].yGNLNDsASNIGk.[V] N-Term(TMT6plex); S7(Phospho); K13(TMT6plex) 1 P38041 P38041 0 3 0.1445 0 1 1 630.98299 1890.93441 1890.93628 -0.99 -0.00062 1618803.625 CID (Collision Induced Dissociation) MS2 26.92442 70 81.0 34.011 77.0214 24045 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 22.9 61.7 110.1 33.5 66.4 165.0 30.7 75.6 152.4 91.7 3.53 1 0.0003003 0.000233 False High Sequest HT (A2) Unambiguous ATLEEAEGESGVEDDAATGSSNK [K].aTLEEAEGEsGVEDDAATGSSNk.[L] N-Term(TMT6plex); S10(Phospho); K23(TMT6plex) 1 P21304 P21304 0 3 0.1925 0 1 1 935.76246 2805.27284 2805.27154 0.46 0.00043 4286598.5 CID (Collision Induced Dissociation) MS2 2.557749 90 76.7 40.047 76.8092 23964 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 22.6 58.7 110.6 19.2 63.7 154.1 28.8 71.8 156.4 81.2 8.52 1 0.0003003 6.901e-09 False High Sequest HT (A2) Unambiguous SKPIPIMPASPQK [K].skPIPImPAsPQk.[G] N-Term(TMT6plex); K2(TMT6plex); M7(Oxidation); S10(Phospho); K13(TMT6plex) 9 O00429 O00429-4; O00429-9; O00429; O00429-6; O00429-8; O00429-7; O00429-3; O00429-5; O00429-2 0 4 0.5013 0 1 1 545.06456 2177.23643 2177.23722 -0.37 -0.0002 739378.6875 CID (Collision Induced Dissociation) MS2 0.0 50 203.9 60.0 76.666 23911 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 221.2 201.4 169.7 227.5 201.3 178.4 245.7 202.1 198.7 193.0 Excluded by Method 3.89 1 0.0003003 2.181e-05 False High Sequest HT (A2) Unambiguous GSGEDDEEETETTPTSTVPVATIAQETLK [K].gSGEDDEEETETTPtSTVPVATIAQETLk.[V] N-Term(TMT6plex); T15(Phospho); K29(TMT6plex) 1 P12962 P12962 0 4 0.0451 0 1 1 894.18064 3573.70072 3573.69846 0.63 0.00056 908591.375 CID (Collision Induced Dissociation) MS2 0.0 30 36.0 60.0 124.0554 41683 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 12.8 31.2 61.1 11.2 28.5 58.6 14.5 35.6 74.7 31.8 Excluded by Method 5.1 1 0.0003003 0.0001906 False High Sequest HT (A2) Unambiguous VQGEAVSNIQENTQTPTVQEESEEEEVDETGVEVK [K].vQGEAVSNIQENTQTPTVQEEsEEEEVDETGVEVk.[D] N-Term(TMT6plex); S22(Phospho); K35(TMT6plex) 3 Q13765 Q13765; E9PAV3; E9PAV3-2 0 4 0.0816 0 1 1 1100.52071 4399.06101 4399.06016 0.19 0.00021 64056.26953125 CID (Collision Induced Dissociation) MS2 6.46595 40 6.0 60.0 148.9405 50901 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 6.4 6.7 2.7 11.5 3.4 3.1 9.8 6.5 2.5 7.6 Excluded by Method 3.92 1 0.0003003 0.0001563 False High Sequest HT (A2) Unambiguous IGEGTYGVVYK [K].iGEGTyGVVYk.[AG] N-Term(TMT6plex); Y6(Phospho); K11(TMT6plex) 5 P24941 P24941; P06493; Q00526; P24941-2; P06493-2 0 3 0.3858 0 1 1 575.30738 1723.90758 1723.90721 0.21 0.00012 3568862.25 CID (Collision Induced Dissociation) MS2 9.283428 40 146.1 30.043 89.5593 28836 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 148.5 151.8 112.9 157.7 136.3 127.4 173.0 162.1 148.3 143.0 Excluded by Method 3.81 1 0.0003003 0.000342 False High Sequest HT (A2) Unambiguous SPSDSGYSYETIGK [K].sPSDSGYSYETIGk.[T] N-Term(TMT6plex); S1(Phospho); K14(TMT6plex) 1 P46821 P46821 0 2 0.1689 0 1 1 1014.9819 2028.95652 2028.95674 -0.11 -0.00011 3672348.25 CID (Collision Induced Dissociation) MS2 3.126493 50 41.8 47.232 82.5007 26193 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 36.1 41.9 29.3 45.2 38.6 43.2 46.3 50.4 36.2 50.4 Excluded by Method 3.73 1 0.0003003 0.0001027 False High Sequest HT (A2) Unambiguous QGSPDQVSPVSEMTSTSLYQDK [K].qGsPDQVSPVSEMTSTSLYQDk.[Q] N-Term(TMT6plex); S3(Phospho); K22(TMT6plex) 1 P46821 P46821 0 3 0.0825 0 1 1 974.80268 2922.3935 2922.38441 3.11 0.00303 549195.75 CID (Collision Induced Dissociation) MS2 0.0 70 38.2 60.0 109.4972 36295 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 37.2 39.6 30.0 37.6 40.6 37.1 40.9 43.0 42.2 33.4 4.12 1 0.0003003 4.596e-05 False High Sequest HT (A2) Unambiguous ADDEEDLSDENIQPELRGQ [K].aDDEEDLsDENIQPELRGQ.[-] N-Term(TMT6plex); S8(Phospho) 1 P14164 P14164 1 3 0.4876 0 1 1 828.03239 2482.08262 2482.082 0.25 0.00021 7046686.5 CID (Collision Induced Dissociation) MS2 0.0 50 64.3 17.357 82.4017 26153 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 17.7 50.3 100.7 20.2 53.6 124.9 17.9 62.9 129.6 64.9 Excluded by Method 6.03 1 0.0003003 1.36e-05 False High Sequest HT (A2) Unambiguous DFELEETDEEK [K].dFELEETDEEk.[A] N-Term(TMT6plex); K11(TMT6plex) 2 P15108 P02829; P15108 0 3 0.3723 0 1 1 614.64004 1841.90556 1841.90568 -0.06 -4e-05 2840882.0 CID (Collision Induced Dissociation) MS2 11.67182 60 143.6 41.233 82.1066 26044 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 36.5 129.5 202.3 48.8 109.4 258.6 56.5 143.1 301.8 149.4 Excluded by Method 4.11 1 0.0003003 3.358e-05 False High Sequest HT (A2) Unambiguous FVDDSDTSYEEK [K].fVDDSDTsYEEk.[E] N-Term(TMT6plex); S8(Phospho); K12(TMT6plex) 1 Q08951 Q08951 0 2 0.0331 0 1 1 986.94561 1972.88395 1972.88291 0.53 0.00052 1119167.25 CID (Collision Induced Dissociation) MS2 0.0 30 73.8 60.0 82.0559 26025 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 21.8 54.1 91.2 32.0 59.4 136.5 36.4 79.2 145.6 81.7 Excluded by Method 3.32 1 0.0003003 0.0004858 False High Sequest HT (A2) Unambiguous QASASYDSEEEEEGLPMSYDEK [K].qASASYDsEEEEEGLPmSYDEk.[R] N-Term(TMT6plex); S8(Phospho); M17(Oxidation); K22(TMT6plex) 1 Q15059 Q15059 0 3 0.0256 0 1 1 1016.76797 3048.28936 3048.29571 -2.08 -0.00212 354149.0 CID (Collision Induced Dissociation) MS2 0.0 40 28.1 60.0 83.0606 26405 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 32.4 31.0 22.7 24.7 26.7 28.9 32.1 27.1 29.4 26.0 Excluded by Method 5.08 1 0.0003003 1.274e-05 False High Sequest HT (A2) Unambiguous DDSHSAEDSEDEK [K].dDSHsAEDsEDEk.[E] N-Term(TMT6plex); S5(Phospho); S9(Phospho); K13(TMT6plex) 2 Q9H1E3 Q9H1E3; Q9H1E3-2 0 3 0.3767 0 1 1 694.60474 2081.79966 2081.79899 0.32 0.00022 190631.0625 CID (Collision Induced Dissociation) MS2 0.0 50 25.2 60.0 35.2759 8666 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 26.5 26.3 25.8 28.9 23.3 24.5 24.2 22.6 28.2 21.5 Excluded by Method 4.46 1 0.0003003 9.508e-05 False High Sequest HT (A2) Unambiguous AREAAAGIQWSEEETEDEEEEK [K].aREAAAGIQWsEEEtEDEEEEk.[E] N-Term(TMT6plex); S11(Phospho); T15(Phospho); K22(TMT6plex) 4 P46087 P46087-3; P46087-2; P46087; P46087-4 1 3 0.5759 0 1 1 1052.12291 3154.35419 3154.3543 -0.04 -4e-05 740270.875 CID (Collision Induced Dissociation) MS2 0.0 30 64.0 60.0 82.7411 26282 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 56.7 66.0 49.8 68.0 57.1 61.8 73.1 77.5 70.3 60.0 Excluded by Method 4.81 1 0.0003003 2.444e-05 False High Sequest HT (A2) Unambiguous EYVPLDNAEQSTSSSQETK [K].eYVPLDNAEQSTSSsQETk.[E] N-Term(TMT6plex); S15(Phospho); K19(TMT6plex) 1 P33322 P33322 0 3 0.0254 0 1 1 884.42058 2651.24718 2651.24896 -0.67 -0.00059 1379151.0 CID (Collision Induced Dissociation) MS2 11.26095 70 68.8 60.0 82.8261 26316 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 12.5 64.2 108.5 21.3 67.3 131.5 23.2 61.4 130.4 67.9 6.68 1 0.0003003 9.419e-09 False High Sequest HT (A2) Unambiguous VQSLEGEKLSPK [K].vQSLEGEkLsPk.[S] N-Term(TMT6plex); K8(TMT6plex); S10(Phospho); K12(TMT6plex) 1 P46821 P46821 1 4 0.5901 0 1 1 521.30103 2082.18227 2082.18148 0.38 0.0002 339081.0 CID (Collision Induced Dissociation) MS2 18.40565 40 214.7 60.0 82.0216 26012 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 192.9 206.0 161.7 255.5 209.7 188.0 266.0 225.4 228.4 213.6 Excluded by Method 6.27 1 0.0003003 8.938e-07 False High Sequest HT (A2) Unambiguous LDESDENEEALAK [K].lDEsDENEEALAk.[A] N-Term(TMT6plex); S4(Phospho); K13(TMT6plex) 1 Q08492 Q08492 0 3 0.5915 0 1 1 667.65329 2000.94531 2000.94657 -0.63 -0.00042 1314949.25 CID (Collision Induced Dissociation) MS2 8.999179 20 150.6 56.932 81.905 25968 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 40.4 133.2 225.3 47.0 125.7 282.0 52.2 140.1 308.3 152.3 Excluded by Method 4.48 1 0.0003003 3.883e-05 False High Sequest HT (A2) Unambiguous DASQEESLI [K].dASQEEsLI.[-] N-Term(TMT6plex); S7(Phospho) 1 P16140 P16140 0 2 0.5 0 1 1 650.79735 1300.58742 1300.58711 0.24 0.00016 4805363.5 CID (Collision Induced Dissociation) MS2 2.614028 40 201.7 32.197 81.0407 25637 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 43.7 155.6 343.0 61.6 156.6 405.7 62.4 189.9 395.9 202.3 Excluded by Method 2.48 1 0.0003003 0.0002832 False High Sequest HT (A2) Unambiguous SSENVQVDLANFEK [K].ssENVQVDLANFEk.[A] N-Term(TMT6plex); S2(Phospho); K14(TMT6plex) 1 P22147 P22147 0 3 0.0041 0 1 1 706.68907 2118.05267 2118.05204 0.3 0.00021 6754661.5 CID (Collision Induced Dissociation) MS2 1.496342 40 134.4 22.549 111.0231 36866 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 35.9 112.9 204.4 41.2 114.8 260.6 39.8 112.5 280.7 141.5 Excluded by Method 4.85 1 0.0003003 8.194e-06 False High Sequest HT (A2) Unambiguous SAHVTVSGGTPK [K].sAHVTVSGGtPk.[G] N-Term(TMT6plex); T10(Phospho); K12(TMT6plex) 7 Q9BXS6 Q9BXS6-4; Q9BXS6-7; Q9BXS6; Q9BXS6-6; Q9BXS6-2; Q9BXS6-5; Q9BXS6-3 0 3 0.2582 0 1 1 560.30312 1678.89481 1678.89296 1.1 0.00062 473317.71875 CID (Collision Induced Dissociation) MS2 11.74486 70 28.1 60.0 46.1672 12597 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 28.8 26.5 25.4 25.0 28.7 31.3 26.3 31.9 27.7 29.5 4.26 1 0.0003003 8.132e-05 False High Sequest HT (A2) Unambiguous SPAVATSTAAPPPPSSPLPSK [K].sPAVATSTAAPPPPSsPLPSk.[S] N-Term(TMT6plex); S16(Phospho); K21(TMT6plex) 5 Q14157-1 Q14157-1; Q14157-5; Q14157-4; Q14157; Q14157-3 0 3 0.0194 0 1 1 833.44919 2498.33303 2498.33078 0.9 0.00075 807835.4375 CID (Collision Induced Dissociation) MS2 4.281655 40 304.3 60.0 80.8209 25555 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 322.3 304.5 259.9 298.1 301.3 305.4 347.5 338.1 291.8 273.7 Excluded by Method 3.6 1 0.0003003 0.0001799 False High Sequest HT (A2) Unambiguous ESDFSDTLSPSK [K].eSDFSDTLsPSk.[E] N-Term(TMT6plex); S9(Phospho); K12(TMT6plex) 5 Q14BN4 Q14BN4-4; Q14BN4-3; Q14BN4-2; Q14BN4-6; Q14BN4 0 3 0.1379 0 1 1 617.63259 1850.88321 1850.88251 0.38 0.00023 1289778.375 CID (Collision Induced Dissociation) MS2 24.99561 90 119.4 60.0 80.5676 25456 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 121.3 125.4 96.5 131.5 109.7 114.0 126.9 129.2 122.5 116.8 5.22 1 0.0003003 3.056e-05 False High Sequest HT (A2) Unambiguous EAPAEGEAAEPGSPTAAEGEAASAASSTSSPK [K].eAPAEGEAAEPGSPtAAEGEAASAASSTSsPk.[A] N-Term(TMT6plex); T15(Phospho); S30(Phospho); K32(TMT6plex) 1 P29966 P29966 0 3 0.0165 0 1 1 1178.52622 3533.56412 3533.561 0.88 0.00104 600886.5625 CID (Collision Induced Dissociation) MS2 0.0 70 34.3 60.0 81.6815 25883 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 34.2 30.0 28.3 35.4 30.4 33.6 40.8 35.9 37.5 36.4 3.03 1 0.0003003 0.0005151 False High Sequest HT (A2) Unambiguous ATLEEAEGESGVEDDAATGSSNK [K].aTLEEAEGEsGVEDDAATGsSNk.[L] N-Term(TMT6plex); S10(Phospho); S20(Phospho); K23(TMT6plex) 1 P21304 P21304 0 3 0.0825 0 1 1 962.41683 2885.23594 2885.23787 -0.67 -0.00064 467659.28125 CID (Collision Induced Dissociation) MS2 0.0 80 67.3 60.0 81.6068 25855 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 19.8 57.9 93.1 24.7 56.0 110.2 34.0 57.4 143.8 76.0 7.27 1 0.0003003 2.847e-09 False High Sequest HT (A2) Unambiguous DLFDYSPPLHK [K].dLFDYsPPLHk.[N] N-Term(TMT6plex); S6(Phospho); K11(TMT6plex) 3 Q9NYF8 Q9NYF8-3; Q9NYF8; Q9NYF8-2 0 2 0.0177 0 1 1 935.48058 1869.95388 1869.95522 -0.72 -0.00067 3846744.75 CID (Collision Induced Dissociation) MS2 0.0 30 168.3 48.28 110.6502 36726 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 180.5 158.1 132.9 178.5 163.6 164.1 188.1 182.3 166.6 167.9 Excluded by Method 4.52 1 0.0003003 0.0001431 False High Sequest HT (A2) Unambiguous DNGFDQLPVLTEDGK [K].dNGFDQLPVLTEDGk.[L] N-Term(TMT6plex); K15(TMT6plex) 1 P32582 P32582 0 3 0.4755 0 1 1 702.70946 2106.11384 2106.11192 0.91 0.00064 548527.6875 CID (Collision Induced Dissociation) MS2 0.0 50 118.3 60.0 110.7174 36752 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 30.7 99.2 180.2 42.3 98.2 215.3 38.3 118.3 230.3 130.1 Excluded by Method 4.29 1 0.0003003 0.0001289 False High Sequest HT (A2) Unambiguous STELLLGVK [K].sTELLLGVk.[T] N-Term(TMT6plex); S1(Phospho); K9(TMT6plex) 2 Q6R327 Q6R327; Q6R327-3 0 2 0.0854 0 1 1 749.43872 1497.87017 1497.86937 0.54 0.0004 1015992.75 CID (Collision Induced Dissociation) MS2 0.0 60 120.5 60.0 110.7643 36769 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 121.8 115.1 103.8 127.5 120.2 124.8 129.0 125.6 118.8 118.4 Excluded by Method 3.98 1 0.0003003 0.000298 False High Sequest HT (A2) Unambiguous DFDLENDEYELSEEEK [K].dFDLENDEYELsEEEk.[N] N-Term(TMT6plex); S12(Phospho); K16(TMT6plex) 1 Q03661 Q03661 0 3 0.4109 0 1 1 848.04409 2542.11771 2542.11621 0.59 0.0005 884767.75 CID (Collision Induced Dissociation) MS2 17.79185 40 32.2 60.0 109.133 36159 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 11.6 21.8 49.7 11.2 30.0 51.3 11.8 31.5 64.8 38.7 Excluded by Method 5.16 1 0.0003003 3.77e-06 False High Sequest HT (A2) Unambiguous ARLLPEGEETLESDDEK [K].aRLLPEGEETLEsDDEk.[D] N-Term(TMT6plex); S13(Phospho); K17(TMT6plex) 1 Q8N6N3 Q8N6N3 1 3 0.2462 0 1 1 823.74394 2469.21728 2469.2162 0.44 0.00036 2959885.5 CID (Collision Induced Dissociation) MS2 8.375813 30 87.8 32.355 85.6038 27355 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 84.2 93.2 75.4 97.8 78.3 77.7 103.7 93.6 82.4 91.4 Excluded by Method 5.85 1 0.0003003 3.217e-08 False High Sequest HT (A2) Unambiguous TGPFEVGVHVLPK [K].tGPFEVGVHVLPk.[I] N-Term(TMT6plex); T1(Phospho); K13(TMT6plex) 1 P39954 P39954 0 3 0.5929 0 1 1 640.0252 1918.06106 1918.06036 0.37 0.00023 944416.4375 CID (Collision Induced Dissociation) MS2 28.99755 50 75.7 60.0 108.2086 35812 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 26.2 61.1 100.8 30.6 63.8 141.5 45.3 70.9 143.5 73.7 Excluded by Method 4.25 1 0.0003003 3.318e-05 False High Sequest HT (A2) Unambiguous IGPLGLSPK [K].iGPLGLsPk.[K] N-Term(TMT6plex); S7(Phospho); K9(TMT6plex) 2 P0CX53 P30050; P0CX53 0 2 0.4114 0 1 1 710.42247 1419.83766 1419.83767 -0.01 -1e-05 7913382.5 CID (Collision Induced Dissociation) MS2 2.367507 40 194.2 30.742 108.0011 35738 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 171.1 177.0 164.6 217.4 182.4 203.8 215.5 198.1 227.6 184.1 Excluded by Method 2.99 1 0.0003003 0.0003366 False High Sequest HT (A2) Unambiguous VLDEEGSEREFDEDSDEK [K].vLDEEGsEREFDEDsDEk.[E] N-Term(TMT6plex); S7(Phospho); S15(Phospho); K18(TMT6plex) 1 O43719 O43719 1 3 0.515 0 1 1 916.0522 2746.14203 2746.14218 -0.05 -5e-05 2186318.25 CID (Collision Induced Dissociation) MS2 0.0 30 146.5 56.192 85.632 27366 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 158.7 149.0 113.1 167.1 141.3 143.0 162.0 152.7 140.0 138.5 Excluded by Method 4.66 1 0.0003003 0.0003335 False High Sequest HT (A2) Unambiguous LPRTQPDGTSVPGEPASPISQRLPPK [K].lPRTQPDGTSVPGEPAsPISQRLPPk.[V] N-Term(TMT6plex); S17(Phospho); K26(TMT6plex) 3 Q14980 Q14980-5; Q14980-2; Q14980 2 4 0.1226 0 1 1 816.69494 3263.75793 3263.75535 0.79 0.00065 837595.9375 CID (Collision Induced Dissociation) MS2 8.938838 30 64.1 60.0 86.141 27559 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 74.4 63.1 58.4 58.3 58.2 55.3 69.8 75.7 63.0 64.4 Excluded by Method 5.22 1 0.0003003 0.0001034 False High Sequest HT (A2) Unambiguous DTDIGLVNDDANSDEK [K].dTDIGLVNDDANsDEk.[E] N-Term(TMT6plex); S13(Phospho); K16(TMT6plex) 1 Q06106 Q06106 0 3 0.4626 0 1 1 753.68536 2259.04153 2259.04299 -0.64 -0.00048 1115908.625 CID (Collision Induced Dissociation) MS2 21.43202 30 49.5 60.0 83.8978 26722 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 28.7 39.8 66.7 32.5 48.6 65.3 37.8 47.7 78.7 48.8 Excluded by Method 4.28 1 0.0003003 1.313e-05 False High Sequest HT (A2) Unambiguous QREMLMEDVGSEEEQEEEDEAPFQEK [K].qREmLmEDVGsEEEQEEEDEAPFQEk.[D] N-Term(TMT6plex); M4(Oxidation); M6(Oxidation); S11(Phospho); K26(TMT6plex) 2 Q9H1E3 Q9H1E3; Q9H1E3-2 1 4 0.6124 0 1 1 928.65539 3711.59974 3711.59672 0.81 0.00076 2602557.75 CID (Collision Induced Dissociation) MS2 0.0 50 82.4 39.03 83.8513 26704 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 84.1 85.3 67.9 89.7 71.5 76.0 97.3 89.8 80.3 82.0 Excluded by Method 6.27 1 0.0003003 2.327e-05 False High Sequest HT (A2) Unambiguous LCAGIMITASHNPK [K].lcAGImITAsHNPk.[Q] N-Term(TMT6plex); C2(Carbamidomethyl); M6(Oxidation); S10(Phospho); K14(TMT6plex) 2 Q96G03 Q96G03-2; Q96G03 0 3 0.2249 0 1 1 689.68958 2067.05419 2067.05324 0.46 0.00032 3673545.5 CID (Collision Induced Dissociation) MS2 5.986944 20 126.2 35.881 83.832 26697 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 135.0 124.4 94.2 133.1 121.6 121.5 145.5 140.9 122.6 123.5 Excluded by Method 3.69 1 0.0003003 6.316e-05 False High Sequest HT (A2) Unambiguous AYEELSNTDLEFK [K].aYEELsNTDLEFk.[F] N-Term(TMT6plex); S6(Phospho); K13(TMT6plex) 1 P16521 P16521 0 3 0.0857 0 1 1 699.67791 2097.01918 2097.01934 -0.07 -5e-05 4252537.0 CID (Collision Induced Dissociation) MS2 9.131904 30 75.5 22.176 108.9265 36080 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 18.9 64.9 116.2 23.9 59.3 139.2 26.9 71.0 151.3 83.6 Excluded by Method 3.5 1 0.0003003 0.0005118 False High Sequest HT (A2) Unambiguous TLSVAAAFNEDEDSEPEEMPPEAK [K].tLSVAAAFNEDEDsEPEEmPPEAk.[M] N-Term(TMT6plex); S14(Phospho); M19(Oxidation); K24(TMT6plex) 1 Q8WW12 Q8WW12 0 3 0.2746 0 1 1 1054.15196 3160.44134 3160.43214 2.91 0.00307 409538.28125 CID (Collision Induced Dissociation) MS2 15.31073 40 22.2 60.0 109.0874 36142 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 23.4 17.5 20.1 19.6 20.7 23.9 29.3 24.7 20.7 21.9 Excluded by Method 3.97 1 0.0003003 0.0001088 False High Sequest HT (A2) Unambiguous GSPTPGFSTRRGSPTTGFIEQK [K].gsPTPGFsTRRGSPTTGFIEQk.[G] N-Term(TMT6plex); S2(Phospho); S8(Phospho); K22(TMT6plex) 1 Q6ZRV2 Q6ZRV2 2 4 0.1181 0 1 1 732.36202 2926.42625 2926.42656 -0.1 -8e-05 2071501.875 CID (Collision Induced Dissociation) MS2 14.13201 40 64.4 60.0 83.974 26748 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 60.2 62.6 52.6 57.5 62.8 64.5 74.5 74.8 63.7 70.4 Excluded by Method 5.25 1 0.0003003 2.928e-05 False High Sequest HT (A2) Unambiguous LPLTDEQTAEGRKSPAVSEEK [K].lPLTDEQTAEGRksPAVsEEk.[E] N-Term(TMT6plex); K13(TMT6plex); S14(Phospho); S18(Phospho); K21(TMT6plex) 1 P38845 P38845 2 5 0.297 0 1 1 627.32289 3132.58532 3132.5834 0.61 0.00038 661913.4375 CID (Collision Induced Dissociation) MS2 6.872417 30 62.5 50.33 84.3442 26889 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 21.3 51.2 81.4 20.8 52.7 114.3 21.2 65.4 133.0 64.1 Excluded by Method 4.68 1 0.0003003 0.0002699 False High Sequest HT (A2) Unambiguous EALAFDNDESDAQDDANNEK [K].eALAFDNDEsDAQDDANNEk.[E] N-Term(TMT6plex); S10(Phospho); K20(TMT6plex) 1 P38968 P38968 0 4 0.6115 0 1 1 688.05213 2749.18669 2749.18781 -0.41 -0.00028 289071.875 CID (Collision Induced Dissociation) MS2 27.62054 20 66.8 60.0 84.7683 27044 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 40.3 63.0 80.1 42.2 62.9 99.9 53.6 67.0 98.8 60.2 Excluded by Method 5.74 1 0.0003003 3.02e-05 False High Sequest HT (A2) Unambiguous SKPIPIMPASPQK [K].skPIPImPAsPQk.[G] N-Term(TMT6plex); K2(TMT6plex); M7(Oxidation); S10(Phospho); K13(TMT6plex) 9 O00429 O00429-4; O00429-9; O00429; O00429-6; O00429-8; O00429-7; O00429-3; O00429-5; O00429-2 0 3 0.6527 0 1 1 726.41763 2177.23834 2177.23722 0.51 0.00037 718958.1875 CID (Collision Induced Dissociation) MS2 13.90432 50 130.0 60.0 76.2553 23839 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 128.3 132.0 106.2 140.9 135.5 120.6 158.3 142.1 121.0 115.3 Excluded by Method 5.01 1 0.0003003 3.879e-05 False High Sequest HT (A2) Unambiguous VQGEAVSNIQENTQTPTVQEESEEEEVDETGVEVK [K].vQGEAVSNIQENTQTPTVQEEsEEEEVDETGVEVk.[D] N-Term(TMT6plex); S22(Phospho); K35(TMT6plex) 3 Q13765 Q13765; E9PAV3; E9PAV3-2 0 4 0.0543 0 1 1 1100.51556 4399.04042 4399.06016 -4.49 -0.00493 438848.5 CID (Collision Induced Dissociation) MS2 0.0 30 14.5 60.0 113.7713 37893 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 14.4 13.7 11.9 14.6 14.3 12.1 17.2 15.7 13.8 16.8 Excluded by Method 4.42 1 0.0003003 6.12e-05 False High Sequest HT (A2) Unambiguous LDEDEDEDDADLSK [K].lDEDEDEDDADLsk.[Y] N-Term(TMT6plex); S13(Phospho); K14(TMT6plex) 2 O95218 O95218-2; O95218 0 2 0.6096 0 1 1 1073.96908 2146.93089 2146.93171 -0.38 -0.00041 1735472.875 CID (Collision Induced Dissociation) MS2 0.0 50 131.0 60.0 77.0434 24135 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 127.6 118.2 92.7 155.7 138.5 126.8 154.5 138.4 136.1 121.8 Excluded by Method 5.61 1 0.0003003 2.825e-07 False High Sequest HT (A2) Unambiguous EEEAKEESDDDMGFGLFD [K].eEEAkEEsDDDmGFGLFD.[-] N-Term(TMT6plex); K5(TMT6plex); S8(Phospho); M12(Oxidation) 1 P02400 P02400 1 3 0.6528 0 1 1 873.03643 2617.09475 2617.0941 0.25 0.00022 5347470.5 CID (Collision Induced Dissociation) MS2 0.0 30 170.2 25.886 113.486 37786 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 32.5 130.1 250.5 56.2 139.2 330.8 61.4 158.0 360.7 182.2 Excluded by Method 3.6 1 0.0003003 0.0001836 False High Sequest HT (A2) Unambiguous SVSQISVNPGK [K].sVSQISVNPGk.[N] N-Term(TMT6plex); S1(Phospho); K11(TMT6plex) 1 Q12500 Q12500 0 3 0.0053 0 1 1 551.97073 1653.89764 1653.89771 -0.04 -2e-05 1838005.375 CID (Collision Induced Dissociation) MS2 7.742238 60 89.2 60.0 76.7323 24018 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 26.2 60.5 143.4 28.6 75.2 169.2 27.4 80.1 177.5 103.7 Excluded by Method 3.77 1 0.0003003 0.0002227 False High Sequest HT (A2) Unambiguous EDSAPVAAGESESSL [K].eDsAPVAAGESESSL.[-] N-Term(TMT6plex); S3(Phospho) 1 P25567 P25567 0 2 0.5335 0 1 1 879.38756 1757.76785 1757.76799 -0.08 -7e-05 2851287.75 CID (Collision Induced Dissociation) MS2 9.226188 80 171.7 60.0 76.7203 24013 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 39.2 139.3 275.0 48.6 138.6 336.7 52.4 151.4 368.0 168.2 4.93 1 0.0003003 1.161e-05 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVSsDEEF.[-] N-Term(TMT6plex); S9(Phospho) 1 P16521 P16521 0 2 0.0448 0 1 1 885.37144 1769.73561 1769.73562 -0.01 -1e-05 8037133.0 CID (Collision Induced Dissociation) MS2 3.45949 70 144.1 27.707 113.7408 37881 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 36.6 108.7 226.9 44.7 109.4 277.9 42.0 137.7 305.8 150.9 4.69 1 0.0003003 3.253e-07 False High Sequest HT (A2) Unambiguous AAADALSDLEIK [K].aAADALsDLEIk.[D] N-Term(TMT6plex); S7(Phospho); K12(TMT6plex) 1 P09938 P09938 0 2 0.5877 0 1 1 877.97081 1754.93435 1754.93415 0.11 0.0001 4677288.5 CID (Collision Induced Dissociation) MS2 8.881477 40 107.6 38.158 113.3103 37723 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 23.9 88.0 165.5 38.4 96.2 194.8 35.8 91.8 227.1 114.8 Excluded by Method 5.19 1 0.0003003 1.011e-06 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVSsDEEF.[-] N-Term(TMT6plex); S9(Phospho) 1 P16521 P16521 0 2 0.0788 0 1 1 885.37169 1769.7361 1769.73562 0.27 0.00024 3583211.5 CID (Collision Induced Dissociation) MS2 11.12082 40 149.6 44.007 114.8829 38303 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 42.8 120.9 229.2 40.6 126.4 290.2 45.4 143.9 305.8 151.1 Excluded by Method 4.44 1 0.0003003 8.606e-06 False High Sequest HT (A2) Unambiguous DDSHSAEDSEDEK [K].dDSHSAEDsEDEk.[E] N-Term(TMT6plex); S9(Phospho); K13(TMT6plex) 2 Q9H1E3 Q9H1E3; Q9H1E3-2 0 3 0.4273 0 1 1 667.9498 2001.83484 2001.83266 1.09 0.00073 89904.59375 CID (Collision Induced Dissociation) MS2 0.0 40 111.0 60.0 30.8954 7117 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 99.7 115.2 89.0 128.9 112.0 97.5 137.5 115.7 114.4 100.5 Excluded by Method 5.64 1 0.0003003 5.672e-05 False High Sequest HT (A2) Unambiguous VFDDESDEK [K].vFDDEsDEk.[E] N-Term(TMT6plex); S6(Phospho); K9(TMT6plex) 1 O43719 O43719 0 2 0.3005 0 1 1 811.37384 1621.7404 1621.73987 0.33 0.00027 1337995.375 CID (Collision Induced Dissociation) MS2 15.73487 50 280.2 60.0 75.0368 23379 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 292.2 301.9 213.4 322.9 261.7 236.7 338.3 305.6 286.6 242.5 Excluded by Method 3.86 1 0.0003003 7.45e-05 False High Sequest HT (A2) Unambiguous QGSPDQVSPVSEMTSTSLYQDK [K].qGsPDQVsPVSEMTSTSLYQDk.[Q] N-Term(TMT6plex); S3(Phospho); S8(Phospho); K22(TMT6plex) 1 P46821 P46821 0 3 0.3018 0 1 1 1001.45616 3002.35392 3002.35074 1.06 0.00106 518885.4375 CID (Collision Induced Dissociation) MS2 37.67542 60 24.3 60.0 115.162 38404 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 25.4 27.4 25.6 14.2 22.4 25.1 24.5 27.6 28.3 22.6 Excluded by Method 4.34 1 0.0003003 2.135e-05 False High Sequest HT (A2) Unambiguous AANMSDDESSLIK [K].aANmsDDESSLIk.[N] N-Term(TMT6plex); M4(Oxidation); S5(Phospho); K13(TMT6plex) 1 P19880 P19880 0 3 0.3261 0 1 1 645.6444 1934.91865 1934.91825 0.21 0.00013 1523477.5 CID (Collision Induced Dissociation) MS2 15.36066 30 69.9 60.0 74.9473 23346 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 19.9 59.4 102.4 20.8 65.7 123.3 25.8 62.6 141.9 77.1 Excluded by Method 2.76 1 0.0003003 0.0001622 False High Sequest HT (A2) Unambiguous DSSVDAFEK [K].dSsVDAFEk.[E] N-Term(TMT6plex); S3(Phospho); K9(TMT6plex) 1 P09064 P09064 0 2 0.1333 0 1 1 768.37327 1535.73927 1535.73948 -0.13 -0.0001 4474519.5 CID (Collision Induced Dissociation) MS2 0.0 40 200.0 60.0 74.8687 23317 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 51.1 159.5 299.5 69.3 176.2 358.6 80.2 191.2 404.6 210.1 Excluded by Method 4.05 1 0.0003003 1.359e-05 False High Sequest HT (A2) Unambiguous EAPAEGEAAEPGSPTAAEGEAASAASSTSSPK [K].eAPAEGEAAEPGSPTAAEGEAASAASSTSsPk.[A] N-Term(TMT6plex); S30(Phospho); K32(TMT6plex) 1 P29966 P29966 0 3 0.0373 0 1 1 1151.87 3453.59545 3453.59467 0.23 0.00026 552604.0 CID (Collision Induced Dissociation) MS2 0.0 60 58.7 60.0 75.3355 23490 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 53.9 59.7 53.3 68.3 54.5 54.4 70.4 64.8 54.2 53.3 Excluded by Method 4.29 1 0.0003003 1.925e-05 False High Sequest HT (A2) Unambiguous EQAEASIDNLK [K].eQAEAsIDNLk.[N] N-Term(TMT6plex); S6(Phospho); K11(TMT6plex) 1 Q08245 Q08245 0 3 0.4382 0 1 1 585.96978 1755.89479 1755.89302 1.01 0.00059 156156240.0 CID (Collision Induced Dissociation) MS2 4.097012 70 168.4 1.562 75.8511 23681 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 48.7 137.0 256.5 48.1 142.8 328.1 56.8 145.8 342.6 177.7 3.4 1 0.0003003 5.414e-05 False High Sequest HT (A2) Unambiguous EPGEQASVPLSPK [K].ePGEQASVPLsPk.[K] N-Term(TMT6plex); S11(Phospho); K13(TMT6plex) 1 P42858 P42858 0 3 0.4901 0 1 1 626.33277 1876.98377 1876.98217 0.86 0.00054 1110896.25 CID (Collision Induced Dissociation) MS2 4.544278 40 133.1 60.0 75.6449 23606 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 132.5 129.7 110.2 147.0 121.3 120.2 155.4 143.5 139.7 131.6 Excluded by Method 4.06 1 0.0003003 0.0004758 False High Sequest HT (A2) Unambiguous ELSPAGSISK [K].eLsPAGSISk.[N] N-Term(TMT6plex); S3(Phospho); K10(TMT6plex) 4 O95453 O95453-4; O95453-2; O95453-3; O95453 0 2 0.2795 0 1 1 763.91515 1526.82302 1526.82315 -0.08 -6e-05 6400671.0 CID (Collision Induced Dissociation) MS2 0.0 20 267.8 40.108 75.617 23594 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 290.5 275.8 214.3 282.8 249.3 270.7 285.9 279.8 274.9 253.7 Excluded by Method 3.47 1 0.0003003 0.0002391 False High Sequest HT (A2) Unambiguous IVFDDEVSIENK [K].iVFDDEVsIENk.[E] N-Term(TMT6plex); S8(Phospho); K12(TMT6plex) 1 P46669 P46669 0 3 0.4262 0 1 1 649.33617 1945.99395 1945.9924 0.8 0.00052 602828.75 CID (Collision Induced Dissociation) MS2 33.97696 50 70.6 60.0 114.5924 38198 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 22.0 66.5 101.3 29.6 58.9 115.8 41.8 64.8 139.3 65.9 Excluded by Method 4.27 1 0.0003003 0.0001289 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVssDEEF.[-] N-Term(TMT6plex); S8(Phospho); S9(Phospho) 1 P16521 P16521 0 2 0.1283 0 1 1 925.35492 1849.70256 1849.70195 0.33 0.0003 1157135.375 CID (Collision Induced Dissociation) MS2 19.11761 40 78.8 60.0 114.6388 38214 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 19.0 69.1 130.3 16.3 67.8 145.8 21.7 75.0 158.4 84.3 Excluded by Method 3.82 1 0.0003003 0.000248 False High Sequest HT (A2) Unambiguous AEASGEAADEADEADEE [K].aEASGEAADEADEADEE.[-] N-Term(TMT6plex) 1 O13516 O13516 0 2 0.8359 0 1 1 969.4001 1937.79292 1937.79333 -0.21 -0.00021 860787.0625 CID (Collision Induced Dissociation) MS2 0.0 100 87.0 60.0 48.7913 13563 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 25.7 67.1 145.7 28.2 66.4 171.6 25.9 74.1 182.7 82.3 5.79 1 0.0003003 1.464e-08 False High Sequest HT (A2) Unambiguous EQAEASIDNLK [K].eQAEAsIDNLk.[N] N-Term(TMT6plex); S6(Phospho); K11(TMT6plex) 1 Q08245 Q08245 0 3 0.4643 0 1 1 585.96941 1755.89369 1755.89302 0.38 0.00022 535624.1875 CID (Collision Induced Dissociation) MS2 27.73421 50 375.5 60.0 79.4357 25038 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 94.3 323.0 599.3 125.1 310.0 686.8 142.4 353.7 744.2 376.4 Excluded by Method 3.08 1 0.0003003 0.0001244 False High Sequest HT (A2) Unambiguous SRSPGSPVGEGTGSPPK [K].sRSPGsPVGEGTGSPPk.[W] N-Term(TMT6plex); S6(Phospho); K17(TMT6plex) 6 P35611 P35611-2; P35611-6; P35611-4; P35611-3; P35611-5; P35611 1 3 0.0545 0 1 1 712.36802 2135.08952 2135.08982 -0.14 -0.0001 1147494.25 CID (Collision Induced Dissociation) MS2 13.10403 60 146.1 60.0 46.9911 12897 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 151.0 139.5 131.9 155.1 133.5 136.1 159.5 160.5 142.2 151.7 Excluded by Method 5.69 1 0.0003003 1.266e-07 False High Sequest HT (A2) Unambiguous VQGEAVSNIQENTQTPTVQEESEEEEVDETGVEVK [K].vQGEAVSNIQENTQTPTVQEEsEEEEVDETGVEVk.[D] N-Term(TMT6plex); S22(Phospho); K35(TMT6plex) 3 Q13765 Q13765; E9PAV3; E9PAV3-2 0 4 0.1734 0 1 1 1100.51861 4399.05263 4399.06016 -1.71 -0.00188 1008864.4375 CID (Collision Induced Dissociation) MS2 52.41373 50 15.8 60.0 112.3866 37377 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 22.9 16.0 12.4 15.9 13.7 13.8 20.0 14.7 16.9 11.8 Excluded by Method 4.73 1 0.0003003 2.52e-05 False High Sequest HT (A2) Unambiguous FLNINRVCSSDSDLPGSDIK [K].fLNINRVcsSDSDLPGSDIk.[S] N-Term(TMT6plex); C8(Carbamidomethyl); S9(Phospho); K20(TMT6plex) 1 P27466 P27466 1 3 0.067 0 1 1 925.79791 2775.37919 2775.37887 0.11 0.00011 673603.125 CID (Collision Induced Dissociation) MS2 11.76565 50 70.8 40.595 112.4168 37388 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 16.4 61.3 99.1 21.7 57.9 133.4 25.3 69.2 151.0 73.1 Excluded by Method 3.73 1 0.0003003 0.0004719 False High Sequest HT (A2) Unambiguous ASSLPGSLQRSRSDIDVNAAAGAK [K].aSSLPGSLQRsRSDIDVNAAAGAk.[A] N-Term(TMT6plex); S11(Phospho); K24(TMT6plex) 3 O75122 O75122-3; O75122; O75122-2 2 4 0.0134 0 1 1 728.13638 2909.52368 2909.52462 -0.32 -0.00023 1106716.625 CID (Collision Induced Dissociation) MS2 7.373623 30 98.3 60.0 79.0785 24902 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 93.0 103.2 79.0 98.6 91.6 102.9 126.3 103.3 93.7 91.2 Excluded by Method 4.47 1 0.0003003 9.812e-07 False High Sequest HT (A2) Unambiguous NLENDTTSSPTQDLDEK [K].nLENDTTSsPTQDLDEk.[S] N-Term(TMT6plex); S9(Phospho); K17(TMT6plex) 1 Q04279 Q04279 0 3 0.1116 0 1 1 815.71984 2445.14498 2445.14343 0.63 0.00052 905519.5625 CID (Collision Induced Dissociation) MS2 6.546395 80 77.4 60.0 79.003 24874 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 16.8 69.3 112.7 24.6 68.6 156.3 25.2 67.0 157.7 75.8 4.66 1 0.0003003 3.931e-06 False High Sequest HT (A2) Unambiguous DDFESEEEDVK [K].dDFEsEEEDVk.[S] N-Term(TMT6plex); S5(Phospho); K11(TMT6plex) 3 Q7Z6E9 Q7Z6E9-4; Q7Z6E9-2; Q7Z6E9 0 3 0.375 0 1 1 627.2801 1879.82575 1879.82506 0.37 0.00023 231121.046875 CID (Collision Induced Dissociation) MS2 49.99915 50 73.0 60.0 79.6437 25115 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 74.5 75.1 51.3 86.3 65.0 66.6 84.0 76.4 76.7 73.9 Excluded by Method 4.64 1 0.0003003 4.277e-05 False High Sequest HT (A2) Unambiguous GPSNSLNLSTADLSLNDSSYNK [K].gPSNsLNLSTADLSLNDSSYNk.[V] N-Term(TMT6plex); S5(Phospho); K22(TMT6plex) 1 P38261 P38261 0 3 0.0025 0 1 1 945.79789 2835.37912 2835.38137 -0.79 -0.00075 476050.125 CID (Collision Induced Dissociation) MS2 49.42554 90 17.6 60.0 112.1827 37302 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 10.3 14.6 22.4 7.8 14.3 30.8 9.7 16.6 32.7 17.1 4.04 1 0.0003003 0.0002412 False High Sequest HT (A2) Unambiguous ESQIDVLQQDELDSYPDSVK [K].eSQIDVLQQDELDsYPDSVk.[K] N-Term(TMT6plex); S14(Phospho); K20(TMT6plex) 1 Q9NQS3 Q9NQS3 0 3 0.039 0 1 1 949.4636 2846.37624 2846.37489 0.48 0.00045 626783.4375 CID (Collision Induced Dissociation) MS2 0.0 70 29.1 60.0 111.5844 37077 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 26.1 34.2 21.3 32.4 28.8 30.1 27.9 30.1 33.0 26.8 4.61 1 0.0003003 4.881e-06 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVSsDEEF.[-] N-Term(TMT6plex); S9(Phospho) 1 P16521 P16521 0 2 0.0155 0 1 1 885.37169 1769.7361 1769.73562 0.27 0.00024 10318404.0 CID (Collision Induced Dissociation) MS2 0.0 60 187.4 20.755 111.6226 37091 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 43.6 155.8 320.4 57.1 141.4 379.8 63.7 168.2 360.0 184.3 Excluded by Method 4.52 1 0.0003003 7.631e-07 False High Sequest HT (A2) Unambiguous FSVSSNDSQNSLK [K].fSVSSNDSQNsLk.[N] N-Term(TMT6plex); S11(Phospho); K13(TMT6plex) 1 Q06833 Q06833 0 3 0.2571 0 1 1 650.9915 1950.95995 1950.95741 1.3 0.00085 511422.78125 CID (Collision Induced Dissociation) MS2 49.04781 40 102.5 60.0 80.2076 25321 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 29.5 82.8 143.1 35.8 77.3 197.2 44.4 89.8 209.4 115.9 Excluded by Method 4.9 1 0.0003003 7.458e-05 False High Sequest HT (A2) Unambiguous REGPVGGESDSEEMFEK [K].rEGPVGGEsDSEEMFEk.[T] N-Term(TMT6plex); S9(Phospho); K17(TMT6plex) 3 Q9Y4B5 Q9Y4B5-3; Q9Y4B5-2; Q9Y4B5 1 3 0.2609 0 1 1 807.70636 2421.10454 2421.10454 0.0 0.0 884632.0 CID (Collision Induced Dissociation) MS2 7.865674 60 57.9 60.0 80.1309 25293 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 56.0 55.3 46.3 59.2 56.7 58.0 67.5 62.4 58.5 58.9 Excluded by Method 3.91 1 0.0003003 0.0001449 False High Sequest HT (A2) Unambiguous SFNYSPNSSTSEVSSTSASK [K].sFNYsPNSSTSEVSSTSASk.[A] N-Term(TMT6plex); S5(Phospho); K20(TMT6plex) 4 Q5VT52 Q5VT52-5; Q5VT52; Q5VT52-2; Q5VT52-3 0 3 0.0025 0 1 1 869.07385 2605.207 2605.20709 -0.03 -3e-05 1184552.875 CID (Collision Induced Dissociation) MS2 5.837852 50 44.8 60.0 80.0086 25246 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 46.5 45.5 43.6 44.6 38.7 42.4 47.7 46.5 45.8 46.7 Excluded by Method 3.95 1 0.0003003 2.419e-05 False High Sequest HT (A2) Unambiguous QSFDDNDSEELEDK [K].qSFDDNDsEELEDk.[D] N-Term(TMT6plex); S8(Phospho); K14(TMT6plex) 1 O60841 O60841 0 3 0.1854 0 1 1 736.99129 2208.95931 2208.95859 0.33 0.00024 989788.8125 CID (Collision Induced Dissociation) MS2 45.84922 50 67.1 56.014 79.9051 25208 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 69.0 69.4 54.3 71.2 62.9 60.3 83.2 65.9 73.1 61.9 Excluded by Method 3.83 1 0.0003003 0.0002707 False High Sequest HT (A2) Unambiguous EENLQQEDDGEVESSGALEK [K].eENLQQEDDGEVEsSGALEk.[K] N-Term(TMT6plex); S14(Phospho); K20(TMT6plex) 1 P46949 P46949 0 3 0.0309 0 1 1 915.42439 2744.25861 2744.25517 1.26 0.00115 664887.3125 CID (Collision Induced Dissociation) MS2 7.669886 80 46.6 60.0 77.3808 24265 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 11.8 37.9 68.1 17.6 38.0 85.4 21.7 44.5 92.1 48.5 7.45 1 0.0003003 5.623e-08 False High Sequest HT (A2) Unambiguous NYWDVNDSPIIK [K].nYWDVNDsPIIk.[V] N-Term(TMT6plex); S8(Phospho); K12(TMT6plex) 1 P23201 P23201 0 3 0.4543 0 1 1 668.00729 2002.00732 2002.00872 -0.7 -0.00046 5572180.0 CID (Collision Induced Dissociation) MS2 11.73757 40 126.0 36.511 112.5453 37436 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 31.6 100.9 217.5 36.8 97.6 248.5 40.1 112.0 263.5 111.5 Excluded by Method 4.38 1 0.0003003 4.305e-05 False High Sequest HT (A2) Unambiguous VVVAENFDEIVNNENK [K].vVVAENFDEIVNNENk.[D] N-Term(TMT6plex); K16(TMT6plex) 1 P30101 P30101 0 3 0.4514 0 1 1 764.41484 2291.22998 2291.22835 0.71 0.00054 2330262.5 CID (Collision Induced Dissociation) MS2 14.40875 60 103.4 59.486 112.5613 37443 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 105.1 106.7 88.6 105.5 90.2 96.2 111.8 116.6 114.7 98.7 Excluded by Method 5.14 1 0.0003003 7.671e-08 False High Sequest HT (A2) Unambiguous RAASEESDVEEDEDK [K].rAASEEsDVEEDEDk.[L] N-Term(TMT6plex); S7(Phospho); K15(TMT6plex) 1 Q04660 Q04660 1 3 0.1331 0 1 1 749.67377 2247.00676 2247.0066 0.07 5e-05 3096273.5 CID (Collision Induced Dissociation) MS2 0.0 40 145.6 60.0 47.5892 13117 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 57.4 123.8 187.5 71.8 128.3 236.2 83.3 148.5 270.9 148.1 Excluded by Method 5.56 1 0.0003003 2.122e-06 False High Sequest HT (A2) Unambiguous SSSPELVTHLK [K].sSsPELVTHLk.[W] N-Term(TMT6plex); S3(Phospho); K11(TMT6plex) 1 Q07960 Q07960 0 3 0.0311 0 1 1 579.31805 1735.93961 1735.93957 0.02 1e-05 5657590.0 CID (Collision Induced Dissociation) MS2 5.725599 90 116.8 34.522 77.839 24435 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 123.3 121.3 94.8 109.9 103.1 121.4 125.1 127.0 115.4 126.6 4.5 1 0.0003003 0.000153 False High Sequest HT (A2) Unambiguous AAQQDSSEDENIGGEYYTK [K].aAQQDsSEDENIGGEYYTk.[L] N-Term(TMT6plex); S6(Phospho); K19(TMT6plex) 1 P25037 P25037 0 3 0.009 0 1 1 881.73552 2643.19201 2643.18636 2.14 0.00188 668741.5 CID (Collision Induced Dissociation) MS2 0.0 70 61.3 60.0 77.6981 24384 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 19.9 46.6 96.5 21.9 51.0 117.1 26.1 55.3 114.6 64.5 4.43 1 0.0003003 1.526e-05 False High Sequest HT (A2) Unambiguous GRLVREDENDASDDEDDDEK [K].gRLVREDENDAsDDEDDDEk.[R] N-Term(TMT6plex); S12(Phospho); K20(TMT6plex) 4 Q9Y5B6 Q9Y5B6-4; Q9Y5B6-3; Q9Y5B6; Q9Y5B6-2 2 4 0.5252 0 1 1 715.81846 2860.25201 2860.2522 -0.07 -5e-05 1145274.5 CID (Collision Induced Dissociation) MS2 0.0 40 43.0 60.0 47.9774 13263 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 44.0 43.4 34.8 46.5 46.1 42.3 46.2 39.9 42.8 44.1 Excluded by Method 6.34 1 0.0003003 8.036e-07 False High Sequest HT (A2) Unambiguous VTFSVSPIEATEK [K].vTFSVsPIEATEk.[C] N-Term(TMT6plex); S6(Phospho); K13(TMT6plex) 3 Q9ULD2 Q9ULD2-5; Q9ULD2; Q9ULD2-2 0 3 0.0843 0 1 1 649.34795 1946.02929 1946.02878 0.26 0.00017 791681.375 CID (Collision Induced Dissociation) MS2 25.51625 50 61.4 60.0 113.2038 37683 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 64.3 52.9 60.0 56.6 52.8 64.1 61.8 72.6 70.8 57.8 Excluded by Method 3.56 1 0.0003003 0.0004165 False High Sequest HT (A2) Unambiguous GEADDEDDDEDGQDNQGTVTEGSSPAYLK [K].gEADDEDDDEDGQDNQGTVTEGSsPAYLk.[E] N-Term(TMT6plex); S24(Phospho); K29(TMT6plex) 9 Q86U86 Q86U86-5; Q86U86-7; Q86U86-4; Q86U86; Q86U86-6; Q86U86-9; Q86U86-8; Q86U86-2; Q86U86-3 0 3 0.0024 0 1 1 1199.17623 3595.51412 3595.51212 0.56 0.00067 541496.75 CID (Collision Induced Dissociation) MS2 0.0 50 10.8 60.0 78.2018 24573 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 9.6 10.3 6.7 10.3 11.3 10.9 13.4 13.9 9.6 12.2 Excluded by Method 4.16 1 0.0003003 0.0002539 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVSsDEEF.[-] N-Term(TMT6plex); S9(Phospho) 1 P16521 P16521 0 2 0.0341 0 1 1 885.37156 1769.73585 1769.73562 0.13 0.00011 19342426.0 CID (Collision Induced Dissociation) MS2 0.0 70 230.8 11.72 112.6701 37483 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 49.0 189.9 380.6 62.1 174.5 470.5 74.0 203.3 475.6 228.5 4.4 1 0.0003003 1.242e-06 False High Sequest HT (A2) Unambiguous VQGEAVSNIQENTQTPTVQEESEEEEVDETGVEVK [K].vQGEAVSNIQENTQTPTVQEEsEEEEVDETGVEVk.[D] N-Term(TMT6plex); S22(Phospho); K35(TMT6plex) 3 Q13765 Q13765; E9PAV3; E9PAV3-2 0 4 0.015 0 1 1 1100.52069 4399.06093 4399.06016 0.18 0.00019 3950557.25 CID (Collision Induced Dissociation) MS2 0.0 50 60.3 27.999 100.8082 33068 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 60.4 63.1 46.8 63.6 52.8 61.4 67.6 67.3 59.6 60.3 Excluded by Method 7.32 1 0.0003003 3.804e-06 False High Sequest HT (A2) Unambiguous DNLSDTEEVIVIK [K].dNLsDTEEVIVIk.[K] N-Term(TMT6plex); S4(Phospho); K13(TMT6plex) 1 Q08951 Q08951 0 2 0.1401 0 1 1 1007.03336 2013.05945 2013.05572 1.85 0.00186 1215328.875 CID (Collision Induced Dissociation) MS2 0.0 30 45.3 60.0 112.8668 37557 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 13.2 38.9 67.8 14.0 34.3 84.8 20.4 40.8 92.6 45.9 Excluded by Method 5.14 1 0.0003003 1.102e-06 False High Sequest HT (A2) Unambiguous QETVADFTPK [K].qETVADFtPk.[K] N-Term(TMT6plex); T8(Phospho); K10(TMT6plex) 3 O75400 O75400-2; O75400-3; O75400 0 3 0.4828 0 1 1 558.62337 1673.85557 1673.85517 0.23 0.00013 1809505.625 CID (Collision Induced Dissociation) MS2 2.587291 80 117.6 60.0 78.4239 24655 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 109.8 127.0 86.8 126.8 119.9 111.7 130.4 130.0 123.4 110.2 4.08 1 0.0003003 5.859e-06 False High Sequest HT (A2) Unambiguous QSFDDNDSEELEDK [K].qSFDDNDsEELEDk.[D] N-Term(TMT6plex); S8(Phospho); K14(TMT6plex) 1 O60841 O60841 0 2 0.4567 0 1 1 1104.98309 2208.9589 2208.95859 0.14 0.00015 1421223.25 CID (Collision Induced Dissociation) MS2 0.0 60 43.2 60.0 78.29 24605 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 41.1 43.3 39.2 53.3 39.4 45.5 49.3 39.2 41.8 40.1 Excluded by Method 5.08 1 0.0003003 2.878e-08 False High Sequest HT (A2) Unambiguous VFVGGLSPDTSEEQIK [K].vFVGGLsPDTSEEQIk.[E] N-Term(TMT6plex); S7(Phospho); K16(TMT6plex) 3 O14979 O14979-3; O14979; O14979-2 0 3 0.1481 0 1 1 748.72382 2244.15691 2244.1565 0.18 0.00014 414729.625 CID (Collision Induced Dissociation) MS2 19.94054 40 99.1 60.0 112.9671 37595 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 103.1 102.1 84.4 97.3 90.7 95.1 112.7 110.3 97.8 97.9 Excluded by Method 4.59 1 0.0003003 0.0003286 False High Sequest HT (A2) Unambiguous ATEETGQAQSGQANCQGLSPVSVASK [K].aTEETGQAQSGQANcQGLsPVSVASk.[N] N-Term(TMT6plex); C15(Carbamidomethyl); S19(Phospho); K26(TMT6plex) 3 Q9UHF7 Q9UHF7-3; Q9UHF7-2; Q9UHF7 0 4 0.2028 0 1 1 786.63284 3143.50952 3143.50804 0.47 0.00037 725779.9375 CID (Collision Induced Dissociation) MS2 16.93218 50 18.0 60.0 78.2467 24589 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 17.9 23.8 12.3 21.5 14.7 20.6 15.9 18.9 18.4 16.1 Excluded by Method 4.98 1 0.0003003 1.62e-05 False High Sequest HT (A2) Unambiguous KPYRIESDEEEDFENVGK [K].kPYRIEsDEEEDFENVGk.[V] N-Term(TMT6plex); K1(TMT6plex); S7(Phospho); K18(TMT6plex) 3 Q96T23 Q96T23; Q96T23-3; Q96T23-2 1 4 0.0338 0 1 1 738.6227 2951.46895 2951.46426 1.59 0.00117 1769246.375 CID (Collision Induced Dissociation) MS2 4.8168 20 305.1 60.0 86.4753 27684 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 338.7 305.4 245.4 311.2 287.5 271.4 359.5 340.5 302.9 288.3 Excluded by Method 4.74 1 0.0003003 0.0002211 False High Sequest HT (A2) Unambiguous GPTTGEGALDLSDVHSPPKSPEGK [K].gPTTGEGALDLSDVHsPPksPEGk.[T] N-Term(TMT6plex); S16(Phospho); K19(TMT6plex); S20(Phospho); K24(TMT6plex) 2 O95684 O95684-2; O95684 1 4 0.4308 0 1 1 806.65345 3223.59196 3223.58922 0.85 0.00068 944156.1875 CID (Collision Induced Dissociation) MS2 9.036413 50 86.8 60.0 86.5718 27721 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 83.8 88.5 70.1 89.5 84.5 87.8 102.3 84.2 94.5 82.3 Excluded by Method 7.01 1 0.0003003 1.886e-05 False High Sequest HT (A2) Unambiguous DFSEDLNSFDK [K].dFSEDLNsFDk.[Q] N-Term(TMT6plex); S8(Phospho); K11(TMT6plex) 1 Q04773 Q04773 0 3 0.4593 0 1 1 618.95675 1854.85568 1854.8563 -0.33 -0.0002 854968.125 CID (Collision Induced Dissociation) MS2 7.328164 60 164.7 60.0 102.8113 33809 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 45.3 130.1 244.9 59.4 139.9 307.5 58.5 163.4 323.7 174.2 Excluded by Method 4.79 1 0.0003003 2.29e-06 False High Sequest HT (A2) Unambiguous VVDYSQFQESDDADEDYGRDSGPPTK [K].vVDYSQFQEsDDADEDYGRDSGPPTk.[K] N-Term(TMT6plex); S10(Phospho); K26(TMT6plex) 1 Q9H1E3 Q9H1E3 1 4 0.103 0 1 1 865.38849 3458.53214 3458.53134 0.23 0.0002 644442.5625 CID (Collision Induced Dissociation) MS2 13.27419 70 30.7 60.0 96.4675 31436 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 34.4 25.7 31.4 27.2 26.9 33.1 31.5 32.5 31.5 32.8 5.63 1 0.0003003 3.926e-06 False High Sequest HT (A2) Unambiguous EVQSILTQEVDNNNK [K].eVQSILTQEVDNNNk.[T] N-Term(TMT6plex); K15(TMT6plex) 1 P47119 P47119 0 3 0.3616 0 1 1 730.39858 2189.1812 2189.1814 -0.09 -7e-05 5501473.0 CID (Collision Induced Dissociation) MS2 0.6442063 60 140.7 22.36 96.5778 31477 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 33.4 114.8 222.9 43.2 118.3 281.4 47.2 126.3 265.7 153.4 Excluded by Method 6.83 1 0.0003003 9.865e-08 False High Sequest HT (A2) Unambiguous TSPENVQDRFALVTPK [K].tsPENVQDRFALVTPk.[K] N-Term(TMT6plex); S2(Phospho); K16(TMT6plex) 1 P49792 P49792 1 3 0.0517 0 1 1 780.7504 2340.23664 2340.23648 0.07 5e-05 7430772.5 CID (Collision Induced Dissociation) MS2 5.026182 50 67.2 23.655 96.0269 31270 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 70.9 63.4 53.2 79.2 66.2 66.2 74.1 69.4 60.6 68.6 Excluded by Method 3.48 1 0.0003003 0.0005304 False High Sequest HT (A2) Unambiguous KTESPLENVAELK [K].kTEsPLENVAELk.[K] N-Term(TMT6plex); K1(TMT6plex); S4(Phospho); K13(TMT6plex) 1 P35732 P35732 1 3 0.08 0 1 1 742.41792 2225.2392 2225.23973 -0.24 -0.00017 1892536.25 CID (Collision Induced Dissociation) MS2 4.836651 50 57.0 60.0 96.5951 31484 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 17.8 41.6 80.0 20.8 51.9 111.2 26.4 51.3 107.9 61.3 Excluded by Method 4.5 1 0.0003003 0.0001445 False High Sequest HT (A2) Unambiguous EQSQENILQEPDLK [K].eQsQENILQEPDLk.[D] N-Term(TMT6plex); S3(Phospho); K14(TMT6plex) 1 P40340 P40340 0 3 0.4044 0 1 1 737.04378 2209.11678 2209.11536 0.64 0.00047 1638039.25 CID (Collision Induced Dissociation) MS2 8.986236 60 60.6 47.511 97.3652 31774 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 19.8 55.5 79.3 22.3 52.1 109.7 24.5 54.1 122.5 66.4 Excluded by Method 4.55 1 0.0003003 0.0003474 False High Sequest HT (A2) Unambiguous IGEGTYGVVYK [K].iGEGtYGVVYk.[AG] N-Term(TMT6plex); T5(Phospho); K11(TMT6plex) 5 P24941 P24941; P06493; Q00526; P24941-2; P06493-2 0 3 0.0374 0 1 1 575.30713 1723.90685 1723.90721 -0.21 -0.00012 7421060.5 CID (Collision Induced Dissociation) MS2 1.675319 30 145.1 23.161 97.071 31662 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 142.7 136.1 115.6 150.0 128.5 150.1 162.3 163.0 148.4 154.2 Excluded by Method 4.28 1 0.0003003 0.0005383 False High Sequest HT (A2) Unambiguous SEADPEGLSNSNIVNDADNINSISK [K].sEADPEGLSNSNIVNDADNINSIsk.[T] N-Term(TMT6plex); S24(Phospho); K25(TMT6plex) 1 Q06685 Q06685 0 3 0.0541 0 1 1 1047.83776 3141.49873 3141.49892 -0.06 -6e-05 811879.1875 CID (Collision Induced Dissociation) MS2 0.0 60 49.2 60.0 102.4456 33671 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 12.2 41.1 69.9 17.2 37.3 98.8 16.1 46.4 97.2 55.4 Excluded by Method 7.58 1 0.0003003 1.563e-08 False High Sequest HT (A2) Unambiguous SPSLSPSPPSPLEK [K].sPsLsPSPPSPLEk.[T] N-Term(TMT6plex); S3(Phospho); S5(Phospho); K14(TMT6plex) 1 P46821 P46821 0 3 0.0277 0 1 1 681.00593 2041.00325 2041.00601 -1.35 -0.00092 794999.5 CID (Collision Induced Dissociation) MS2 0.0 40 189.9 60.0 96.9077 31602 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 205.8 195.9 163.0 182.9 182.7 179.6 215.0 196.5 191.3 186.0 Excluded by Method 3.25 1 0.0003003 0.0001886 False High Sequest HT (A2) Unambiguous KDDSHSAEDSEDEK [K].kDDSHsAEDsEDEk.[E] N-Term(TMT6plex); K1(TMT6plex); S6(Phospho); S10(Phospho); K14(TMT6plex) 2 Q9H1E3 Q9H1E3; Q9H1E3-2 1 3 0.3699 0 1 1 813.69061 2439.05727 2439.05689 0.16 0.00013 318974.34375 CID (Collision Induced Dissociation) MS2 0.0 60 199.1 60.0 39.3583 10135 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 198.0 210.7 142.8 239.1 179.2 171.2 251.1 209.7 214.8 174.7 Excluded by Method 5.92 1 0.0003003 0.0003092 False High Sequest HT (A2) Unambiguous FGSNINLEADES [K].fGSNINLEADES.[-] N-Term(TMT6plex) 1 Q9NQP4 Q9NQP4 0 2 0.6473 0 1 1 762.87243 1524.73757 1524.73793 -0.24 -0.00018 877514.125 CID (Collision Induced Dissociation) MS2 16.08622 100 90.7 60.0 94.5236 30702 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 88.7 94.4 85.9 92.1 82.1 92.1 96.4 87.2 86.3 101.3 2.41 1 0.0003003 0.0002351 False High Sequest HT (A2) Unambiguous GFSVVADTPELQRIK [K].gFSVVADtPELQRIk.[K] N-Term(TMT6plex); T8(Phospho); K15(TMT6plex) 3 Q14847 Q14847-2; Q14847-3; Q14847 1 3 0.2735 0 1 1 733.40487 2198.20005 2198.19864 0.64 0.00047 2099453.0 CID (Collision Induced Dissociation) MS2 3.431653 20 131.7 38.333 103.6827 34143 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 147.3 128.6 119.4 129.1 121.2 131.5 148.9 138.8 115.7 136.5 Excluded by Method 5.96 1 0.0003003 1.317e-05 False High Sequest HT (A2) Unambiguous TQSSESRLYELSAEDLEK [K].tQsSESRLYELSAEDLEk.[E] N-Term(TMT6plex); S3(Phospho); K18(TMT6plex) 1 P53885 P53885 1 3 0.0151 0 1 1 875.1007 2623.28755 2623.29043 -1.1 -0.00096 788943.4375 CID (Collision Induced Dissociation) MS2 8.662188 50 25.2 60.0 103.7577 34172 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 12.6 22.8 33.2 15.5 18.0 38.9 13.8 25.8 44.6 27.0 Excluded by Method 4.63 1 0.0003003 0.0004379 False High Sequest HT (A2) Unambiguous GEDPYISLPNQEDSQEEK [K].gEDPYISLPNQEDsQEEk.[Q] N-Term(TMT6plex); S14(Phospho); K18(TMT6plex) 1 P21304 P21304 0 3 0.4369 0 1 1 872.74182 2616.2109 2616.21184 -0.36 -0.00032 1114757.625 CID (Collision Induced Dissociation) MS2 6.268703 60 101.6 60.0 93.3843 30274 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 26.9 75.6 152.7 31.5 83.4 190.0 32.7 92.7 217.6 113.3 Excluded by Method 5.15 1 0.0003003 1.125e-05 False High Sequest HT (A2) Unambiguous DNEELQEIDNVSLDEPK [K].dNEELQEIDNVsLDEPk.[I] N-Term(TMT6plex); S12(Phospho); K17(TMT6plex) 1 P25037 P25037 0 3 0.5601 0 1 1 842.40719 2525.20703 2525.20603 0.39 0.00033 3621420.0 CID (Collision Induced Dissociation) MS2 0.0 50 109.1 31.815 103.8151 34193 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 26.7 91.7 151.5 41.8 92.7 191.2 41.2 108.1 235.4 110.8 Excluded by Method 5.57 1 0.0003003 1.289e-07 False High Sequest HT (A2) Unambiguous VQGEAVSNIQENTQTPTVQEESEEEEVDETGVEVK [K].vQGEAVSNIQENTQTPTVQEEsEEEEVDETGVEVk.[D] N-Term(TMT6plex); S22(Phospho); K35(TMT6plex) 3 Q13765 Q13765; E9PAV3; E9PAV3-2 0 4 0.0559 0 1 1 1100.52118 4399.06288 4399.06016 0.62 0.00068 968469.1875 CID (Collision Induced Dissociation) MS2 0.0 30 31.7 60.0 103.8277 34199 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 32.2 34.2 29.3 30.6 24.8 33.2 34.6 33.2 31.9 32.8 Excluded by Method 5.72 1 0.0003003 4.767e-06 False High Sequest HT (A2) Unambiguous GILAADESTGSIAK [K].gILAADEsTGSIAk.[R] N-Term(TMT6plex); S8(Phospho); K14(TMT6plex) 2 P04075 P04075; P04075-2 0 2 0.0475 0 1 1 936.00103 1870.99478 1870.99273 1.09 0.00102 773590.5625 CID (Collision Induced Dissociation) MS2 11.87193 20 27.5 60.0 93.6829 30388 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 20.6 26.3 25.9 24.6 27.0 31.8 29.2 24.5 32.9 32.0 Excluded by Method 3.79 1 0.0003003 0.0003354 False High Sequest HT (A2) Unambiguous DLRQLDANSSTTALDALNK [K].dLRQLDANSsTTALDALNk.[E] N-Term(TMT6plex); S10(Phospho); K19(TMT6plex) 1 P34163 P34163 1 3 0.0242 0 1 1 862.11756 2584.33813 2584.33838 -0.1 -8e-05 2836418.25 CID (Collision Induced Dissociation) MS2 45.25248 70 56.7 21.594 103.46 34057 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 18.2 44.8 82.3 17.7 46.0 103.6 23.3 57.0 114.0 60.0 5.38 1 0.0003003 3.634e-05 False High Sequest HT (A2) Unambiguous QREMLMEDVGSEEEQEEEDEAPFQEK [K].qREMLmEDVGsEEEQEEEDEAPFQEk.[D] N-Term(TMT6plex); M6(Oxidation); S11(Phospho); K26(TMT6plex) 2 Q9H1E3 Q9H1E3; Q9H1E3-2 1 3 0.5889 0 1 1 1232.53898 3695.6024 3695.6018 0.16 0.0002 584028.1875 CID (Collision Induced Dissociation) MS2 0.0 40 54.2 60.0 95.2231 30967 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 51.1 61.0 41.9 57.4 53.7 53.5 66.3 48.9 55.9 52.7 Excluded by Method 5.4 1 0.0003003 1.454e-06 False High Sequest HT (A2) Unambiguous YSPKTPPRWAEDQNSLLK [K].ySPktPPRWAEDQNSLLk.[M] N-Term(TMT6plex); Y1(Phospho); K4(TMT6plex); T5(Phospho); K18(TMT6plex) 3 P49792 P49792; A6NKT7; Q7Z3J3 2 4 0.3917 0 1 1 745.13587 2977.52166 2977.51929 0.8 0.00059 1122092.25 CID (Collision Induced Dissociation) MS2 0.0 50 145.9 58.921 103.1647 33944 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 155.9 132.2 110.2 159.8 141.7 148.2 172.4 146.9 151.1 140.6 Excluded by Method 4.34 1 0.0003003 0.0005346 False High Sequest HT (A2) Unambiguous VVDYSQFQESDDADEDYGRDSGPPTK [K].vVDYSQFQEsDDADEDYGRDSGPPTk.[K] N-Term(TMT6plex); S10(Phospho); K26(TMT6plex) 1 Q9H1E3 Q9H1E3 1 4 0.1611 0 1 1 865.3877 3458.52896 3458.53134 -0.69 -0.00059 484546.46875 CID (Collision Induced Dissociation) MS2 17.81382 30 42.9 60.0 95.1494 30938 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 39.5 43.4 38.4 47.7 36.8 36.1 44.8 50.0 43.7 48.5 Excluded by Method 5.71 1 0.0003003 1.874e-05 False High Sequest HT (A2) Unambiguous LFIIRGSPQQIDHAK [K].lFIIRGsPQQIDHAk.[Q] N-Term(TMT6plex); S7(Phospho); K15(TMT6plex) 1 Q92945 Q92945 1 4 0.5847 0 1 1 566.06959 2261.25655 2261.25716 -0.27 -0.00015 772914.1875 CID (Collision Induced Dissociation) MS2 0.0 30 116.6 60.0 95.1223 30927 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 113.1 109.6 108.9 121.4 111.8 114.2 124.1 123.1 118.9 121.1 Excluded by Method 4.31 1 0.0003003 0.0001893 False High Sequest HT (A2) Unambiguous ETTSDNEDLLIK [K].eTTsDNEDLLIk.[E] N-Term(TMT6plex); S4(Phospho); K12(TMT6plex) 1 Q04347 Q04347 0 2 0.096 0 1 1 958.48642 1915.96556 1915.96658 -0.53 -0.00051 5102051.0 CID (Collision Induced Dissociation) MS2 37.97664 30 166.2 31.183 95.1181 30925 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 42.2 135.2 253.3 55.3 133.6 313.6 56.7 149.9 338.9 183.2 Excluded by Method 5.0 1 0.0003003 0.000157 False High Sequest HT (A2) Unambiguous FEDEGAGFEESSETGDYEEK [K].fEDEGAGFEESsETGDYEEk.[A] N-Term(TMT6plex); S12(Phospho); K20(TMT6plex) 1 P46821 P46821 0 3 0.0655 0 1 1 931.72766 2793.16841 2793.17043 -0.72 -0.00067 406454.75 CID (Collision Induced Dissociation) MS2 18.07562 80 33.1 60.0 94.9487 30861 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 35.5 32.9 34.8 34.5 29.3 31.7 40.2 32.4 32.1 28.0 5.19 1 0.0003003 1.164e-06 False High Sequest HT (A2) Unambiguous SLKESEQESEEEILAQK [K].sLkEsEQEsEEEILAQk.[K] N-Term(TMT6plex); K3(TMT6plex); S5(Phospho); S9(Phospho); K17(TMT6plex) 1 Q9BW71 Q9BW71 1 4 0.1245 0 1 1 706.85216 2824.38681 2824.38733 -0.18 -0.00013 1152177.625 CID (Collision Induced Dissociation) MS2 2.569436 20 221.2 60.0 94.8488 30823 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 234.9 209.1 177.8 242.5 209.2 203.7 284.4 231.2 207.5 212.2 Excluded by Method 5.14 1 0.0003003 0.0004016 False High Sequest HT (A2) Unambiguous NVSHNPLLLLTPQK [K].nVSHNPLLLLtPQk.[V] N-Term(TMT6plex); T11(Phospho); K14(TMT6plex) 1 P47712 P47712 0 3 0.4266 0 1 1 704.7376 2112.19824 2112.19825 0.0 0.0 840452.1875 CID (Collision Induced Dissociation) MS2 0.0 50 137.9 60.0 103.4112 34039 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 61.8 116.8 217.8 60.9 117.4 225.1 74.6 121.1 259.8 123.9 Excluded by Method 3.54 1 0.0003003 0.0001002 False High Sequest HT (A2) Unambiguous SFDHLISDTK [K].sFDHLISDTk.[A] N-Term(TMT6plex); S1(Phospho); K10(TMT6plex) 3 P21359 P21359-2; P21359-6; P21359 0 3 0.5736 0 1 1 567.62721 1700.86707 1700.86607 0.59 0.00033 996433.875 CID (Collision Induced Dissociation) MS2 18.98731 80 224.1 60.0 94.7892 30800 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 233.4 226.1 186.2 222.3 225.2 225.9 252.4 240.6 206.0 222.7 4.55 1 0.0003003 8.286e-05 False High Sequest HT (A2) Unambiguous DLSSLYSEK [K].dLSSLYsEk.[K] N-Term(TMT6plex); S7(Phospho); K9(TMT6plex) 1 P25558 P25558 0 3 0.2994 0 1 1 527.27215 1579.80189 1579.80208 -0.11 -6e-05 1268076.5 CID (Collision Induced Dissociation) MS2 0.0 70 64.2 60.0 97.5108 31828 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 16.8 54.9 83.2 25.2 50.0 128.9 29.0 59.8 120.2 74.4 3.44 1 0.0003003 8.705e-05 False High Sequest HT (A2) Unambiguous DSIINSPVSGRQSISPTLSNATTTTTK [K].dSIINSPVSGRQsIsPTLSNATTTTTk.[S] N-Term(TMT6plex); S13(Phospho); S15(Phospho); K27(TMT6plex) 1 P36024 P36024 1 3 0.2068 0 1 1 1132.23477 3394.68975 3394.69083 -0.32 -0.00036 306960.625 CID (Collision Induced Dissociation) MS2 0.0 60 29.8 60.0 99.4746 32572 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 12.5 24.1 43.1 9.0 20.9 60.2 12.4 24.7 60.8 30.7 Excluded by Method 4.11 1 0.0003003 0.0002616 False High Sequest HT (A2) Unambiguous SASPEVSEGHENQHGQESEAK [K].sAsPEVSEGHENQHGQESEAk.[A] N-Term(TMT6plex); S1(Phospho); S3(Phospho); K21(TMT6plex) 1 O60930 O60930 0 4 0.4346 0 1 1 714.56205 2855.22638 2855.22865 -0.79 -0.00057 278692.5 CID (Collision Induced Dissociation) MS2 38.05011 50 25.2 60.0 40.1856 10434 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 25.2 29.7 21.4 25.6 22.0 23.2 27.1 22.8 25.2 29.6 Excluded by Method 4.05 1 0.0003003 0.0004547 False High Sequest HT (A2) Unambiguous IEEVLSPEGSPSK [K].iEEVLSPEGsPSk.[S] N-Term(TMT6plex); S10(Phospho); K13(TMT6plex) 2 Q9UEY8 Q9UEY8-2; Q9UEY8 0 3 0.1847 0 1 1 637.33582 1909.99289 1909.9924 0.26 0.00017 7092139.5 CID (Collision Induced Dissociation) MS2 5.769785 50 159.6 18.57 99.1502 32450 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 160.7 154.8 139.4 168.5 152.0 161.9 181.5 163.3 160.5 153.9 Excluded by Method 4.98 1 0.0003003 1.087e-05 False High Sequest HT (A2) Unambiguous NTLETSSLNFK [K].nTLETsSLNFk.[V] N-Term(TMT6plex); S6(Phospho); K11(TMT6plex) 1 Q14151 Q14151 0 3 0.0172 0 1 1 597.98136 1791.92954 1791.9294 0.08 5e-05 363573.6875 CID (Collision Induced Dissociation) MS2 16.91703 50 164.6 60.0 98.8319 32334 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 161.8 166.8 142.9 174.1 152.8 155.3 177.9 169.9 174.7 170.1 Excluded by Method 4.06 1 0.0003003 0.0001432 False High Sequest HT (A2) Unambiguous DIDSEDEQDPFESHFNQVPEK [K].dIDsEDEQDPFESHFNQVPEk.[F] N-Term(TMT6plex); S4(Phospho); K21(TMT6plex) 1 P40498 P40498 0 3 0.3792 0 1 1 1015.12655 3043.36511 3043.36103 1.34 0.00136 1066171.25 CID (Collision Induced Dissociation) MS2 0.0 30 51.9 60.0 101.5357 33331 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 10.3 46.7 75.7 18.3 45.1 94.4 19.7 51.1 109.4 48.1 Excluded by Method 5.01 1 0.0003003 5.057e-05 False High Sequest HT (A2) Unambiguous FEGSDDDENHPLPPLNVAK [K].fEGsDDDENHPLPPLNVAk.[G] N-Term(TMT6plex); S4(Phospho); K19(TMT6plex) 1 P13185 P13185 0 3 0.589 0 1 1 878.09447 2632.26886 2632.26963 -0.29 -0.00026 796842.25 CID (Collision Induced Dissociation) MS2 17.4535 20 67.0 58.61 100.9465 33118 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 21.7 54.6 101.2 24.3 55.5 121.3 20.3 58.1 135.5 77.5 Excluded by Method 6.35 1 0.0003003 2.226e-05 False High Sequest HT (A2) Unambiguous GDNITLLQSVSN [K].gDNITLLQSVSN.[-] N-Term(TMT6plex) 1 P62304 P62304 0 2 0.5309 0 1 1 745.40607 1489.80487 1489.80595 -0.73 -0.00054 2144884.0 CID (Collision Induced Dissociation) MS2 5.782155 80 149.3 49.068 99.8171 32699 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 154.1 146.9 134.4 144.9 141.4 157.3 161.1 165.3 141.7 145.8 2.75 1 0.0003003 0.000233 False High Sequest HT (A2) Unambiguous IEEVLSPEGSPSK [K].iEEVLsPEGsPSk.[S] N-Term(TMT6plex); S6(Phospho); S10(Phospho); K13(TMT6plex) 2 Q9UEY8 Q9UEY8-2; Q9UEY8 0 3 0.5851 0 1 1 663.99167 1989.96046 1989.95873 0.87 0.00058 1005200.9375 CID (Collision Induced Dissociation) MS2 4.416571 60 192.0 60.0 102.2306 33592 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 193.6 194.0 160.9 210.1 181.1 185.0 233.5 196.3 186.4 179.0 Excluded by Method 4.7 1 0.0003003 2.459e-05 False High Sequest HT (A2) Unambiguous DESCYDNAEAAFSDDEEDLNSK [K].dEScYDNAEAAFsDDEEDLNSk.[G] N-Term(TMT6plex); C4(Carbamidomethyl); S13(Phospho); K22(TMT6plex) 4 Q68E01 Q68E01; Q68E01-4; Q68E01-2; Q68E01-3 0 3 0.3032 0 1 1 1021.42287 3062.25407 3062.24982 1.39 0.00141 1109155.25 CID (Collision Induced Dissociation) MS2 0.0 70 81.4 60.0 97.7746 31928 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 75.7 77.1 63.4 83.4 79.3 80.6 94.3 93.8 83.1 83.7 9.07 1 0.0003003 2.609e-09 False High Sequest HT (A2) Unambiguous RGSIGENQGEEK [K].rGsIGENQGEEk.[G] N-Term(TMT6plex); S3(Phospho); K12(TMT6plex) 3 Q05682-2 Q05682-3; Q05682-6; Q05682-2 1 3 0.5658 0 1 1 614.64358 1841.91618 1841.91588 0.17 0.0001 2094315.5 CID (Collision Induced Dissociation) MS2 3.275369 80 154.5 60.0 39.4643 10172 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 156.7 153.8 121.0 173.7 143.4 141.9 172.5 172.2 153.3 156.2 4.56 1 0.0003003 2.106e-05 False High Sequest HT (A2) Unambiguous GPAESPDEGITTTEGEGECEQTPEELEPVEK [K].gPAEsPDEGITTTEGEGEcEQTPEELEPVEk.[Q] N-Term(TMT6plex); S5(Phospho); C19(Carbamidomethyl); K31(TMT6plex) 1 P46821 P46821 0 4 0.1051 0 1 1 971.438 3882.73018 3882.74041 -2.63 -0.00256 473398.8125 CID (Collision Induced Dissociation) MS2 0.0 60 29.2 60.0 97.6568 31883 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 26.9 26.7 24.8 26.5 33.0 31.5 30.4 26.2 30.5 35.5 Excluded by Method 5.33 1 0.0003003 0.0002731 False High Sequest HT (A2) Unambiguous VVDYSQFQESDDADEDYGRDSGPPTK [K].vVDYSQFQEsDDADEDYGRDSGPPTk.[K] N-Term(TMT6plex); S10(Phospho); K26(TMT6plex) 1 Q9H1E3 Q9H1E3 1 4 0.1282 0 1 1 865.38959 3458.53653 3458.53134 1.5 0.0013 620273.125 CID (Collision Induced Dissociation) MS2 0.0 50 18.5 60.0 97.988 32013 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 17.2 19.7 17.4 17.0 18.5 20.6 21.1 16.5 19.5 17.0 Excluded by Method 6.16 1 0.0003003 6.483e-06 False High Sequest HT (A2) Unambiguous FQYIAISQSDADSESCK [K].fQYIAISQSDADSESck.[M] N-Term(TMT6plex); C16(Carbamidomethyl); K17(TMT6plex) 1 P38013 P38013 0 3 0.6272 0 1 1 803.06721 2407.18707 2407.18516 0.79 0.00064 2259747.75 CID (Collision Induced Dissociation) MS2 0.0 90 92.4 47.445 97.9898 32014 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 26.5 74.2 141.9 28.1 67.6 181.1 37.2 85.5 186.7 95.2 5.66 1 0.0003003 1.615e-07 False High Sequest HT (A2) Unambiguous DGVLTLANNVTPAK [K].dGVLTLANNVtPAk.[D] N-Term(TMT6plex); T11(Phospho); K14(TMT6plex) 2 P27816 P27816; P27816-6 0 3 0.5661 0 1 1 651.02708 1951.0667 1951.06656 0.07 5e-05 5468371.5 CID (Collision Induced Dissociation) MS2 3.172004 50 171.2 20.533 98.6296 32257 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 183.3 172.2 134.8 196.4 169.8 158.1 190.5 179.0 166.5 161.0 Excluded by Method 6.13 1 0.0003003 3.355e-06 False High Sequest HT (A2) Unambiguous EEDNIDEDEYEEEGEGEESDFDEYK [K].eEDNIDEDEYEEEGEGEEsDFDEYk.[D] N-Term(TMT6plex); S19(Phospho); K25(TMT6plex) 1 Q06839 Q06839 0 3 0.1311 0 1 1 1194.47469 3581.40952 3581.40525 1.19 0.00142 325698.15625 CID (Collision Induced Dissociation) MS2 0.0 60 8.3 60.0 98.5953 32245 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 2.3 8.5 9.7 9.2 17.0 2.3 7.4 20.8 5.6 Excluded by Method 4.88 1 0.0003003 0.0001089 False High Sequest HT (A2) Unambiguous AAEAGGAEEQYGFLTTPTK [K].aAEAGGAEEQYGFLTtPTk.[Q] N-Term(TMT6plex); T16(Phospho); K19(TMT6plex) 1 P46821 P46821 0 3 0.0478 0 1 1 827.07689 2479.21611 2479.21581 0.12 0.0001 6570395.0 CID (Collision Induced Dissociation) MS2 7.702236 70 103.5 21.679 98.2177 32101 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 96.1 104.7 86.5 103.5 91.8 110.1 111.9 112.3 112.8 105.4 5.23 1 0.0003003 1.784e-08 False High Sequest HT (A2) Unambiguous EVDPSTGELQSLQMPESEGPSSLDPSQEGPTGLK [K].eVDPSTGELQSLQmPESEGPSsLDPSQEGPTGLk.[E] N-Term(TMT6plex); M14(Oxidation); S22(Phospho); K34(TMT6plex) 2 Q13501 Q13501-2; Q13501 0 4 0.0537 0 1 1 1020.98983 4080.93749 4080.92485 3.1 0.00316 1256660.875 CID (Collision Induced Dissociation) MS2 5.81441 30 45.1 60.0 101.8634 33454 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 38.5 43.5 36.1 50.1 47.9 48.0 46.8 51.7 45.8 42.4 Excluded by Method 6.71 1 0.0003003 0.000168 False High Sequest HT (A2) Unambiguous VQGEAVSNIQENTQTPTVQEESEEEEVDETGVEVK [K].vQGEAVSNIQENTQTPTVQEEsEEEEVDETGVEVk.[D] N-Term(TMT6plex); S22(Phospho); K35(TMT6plex) 3 Q13765 Q13765; E9PAV3; E9PAV3-2 0 4 0.0642 0 1 1 1100.52167 4399.06484 4399.06016 1.06 0.00117 1075162.875 CID (Collision Induced Dissociation) MS2 0.0 50 41.7 60.0 102.0107 33509 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 41.8 46.1 26.7 51.5 36.1 39.9 48.3 45.2 39.3 42.3 Excluded by Method 6.7 1 0.0003003 1.434e-06 False High Sequest HT (A2) Unambiguous SHDDGNIDLESDSFLK [K].sHDDGNIDLESDsFLk.[F] N-Term(TMT6plex); S13(Phospho); K16(TMT6plex) 10 P18583 P18583; P18583-4; P18583-6; P18583-5; P18583-10; P18583-9; P18583-2; P18583-3; P18583-7; P18583-8 0 3 0.1307 0 1 1 777.36942 2330.09371 2330.09536 -0.71 -0.00055 830886.875 CID (Collision Induced Dissociation) MS2 6.196641 30 57.8 45.28 98.1218 32064 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 59.9 54.7 52.2 57.6 57.6 53.5 64.2 65.1 58.2 54.9 Excluded by Method 5.05 1 0.0003003 4.691e-05 False High Sequest HT (A2) Unambiguous CTGGGIILTASHNPGGPENDLGIK [K].cTGGGIILTAsHNPGGPENDLGIk.[Y] N-Term(TMT6plex); C1(Carbamidomethyl); S11(Phospho); K24(TMT6plex) 1 P33401 P33401 0 3 0.0764 0 1 1 972.82917 2916.47297 2916.46908 1.33 0.0013 754106.9375 CID (Collision Induced Dissociation) MS2 8.693539 20 68.8 60.0 102.0429 33522 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 16.2 57.9 104.4 24.2 58.6 129.6 25.0 62.6 141.2 68.2 Excluded by Method 7.07 1 0.0003003 1.053e-06 False High Sequest HT (A2) Unambiguous GLIVYCVTSPK [K].gLIVYcVTsPk.[K] N-Term(TMT6plex); C6(Carbamidomethyl); S9(Phospho); K11(TMT6plex) 2 Q8TEU7 Q8TEU7; Q8TEU7-4 0 3 0.1737 0 1 1 592.32482 1774.95989 1774.95787 1.14 0.00068 1715753.375 CID (Collision Induced Dissociation) MS2 46.60937 50 46.6 60.0 106.3806 35143 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 46.6 49.5 33.1 53.3 45.6 51.6 47.0 44.1 49.9 45.2 Excluded by Method 3.57 1 0.0003003 0.0002393 False High Sequest HT (A2) Unambiguous VVDYSQFQESDDADEDYGRDSGPPTK [K].vVDYSQFQEsDDADEDYGRDSGPPTk.[K] N-Term(TMT6plex); S10(Phospho); K26(TMT6plex) 1 Q9H1E3 Q9H1E3 1 4 0.0765 0 1 1 865.39038 3458.53971 3458.53134 2.42 0.00209 610195.875 CID (Collision Induced Dissociation) MS2 67.85905 30 18.6 60.0 88.8283 28564 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 17.3 17.7 18.5 17.9 20.0 19.9 20.9 19.2 18.2 16.5 Excluded by Method 4.97 1 0.0003003 5.488e-05 False High Sequest HT (A2) Unambiguous YDEYFNEYNNNSN [K].yDEYFNEYNNNSN.[-] N-Term(TMT6plex) 1 P32776 P32776 0 2 0.675 0 1 1 957.90286 1914.79844 1914.79797 0.25 0.00024 966891.5625 CID (Collision Induced Dissociation) MS2 0.0 80 47.9 60.0 88.814 28559 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 11.8 40.2 73.7 14.5 42.4 93.7 18.6 43.5 92.4 47.8 3.6 1 0.0003003 8.121e-06 False High Sequest HT (A2) Unambiguous LFDEEEDSSEK [K].lFDEEEDSsEk.[L] N-Term(TMT6plex); S9(Phospho); K11(TMT6plex) 1 O43719 O43719 0 2 0.0068 0 1 1 933.42675 1865.84622 1865.84579 0.23 0.00022 3435422.5 CID (Collision Induced Dissociation) MS2 0.0 20 213.0 60.0 88.6761 28507 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 206.5 215.2 164.8 229.4 195.4 197.7 240.2 229.8 226.3 225.2 Excluded by Method 4.44 1 0.0003003 0.0002787 False High Sequest HT (A2) Unambiguous VPSPLEGSEGDGDTD [K].vPSPLEGSEGDGDtD.[-] N-Term(TMT6plex); T14(Phospho) 2 Q9Y606 Q9Y606; Q9Y606-2 0 3 0.0317 0 1 1 595.25413 1783.74785 1783.74725 0.33 0.0002 441314.09375 CID (Collision Induced Dissociation) MS2 10.78728 30 47.8 60.0 88.623 28487 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 51.2 43.2 54.0 36.6 43.9 54.3 50.2 56.1 34.9 53.7 Excluded by Method 3.47 1 0.0003003 0.0004414 False High Sequest HT (A2) Unambiguous GLQTVVVEEDA [K].gLQTVVVEEDA.[-] N-Term(TMT6plex) 2 P0C2H8 P0C2H8; P0C2H9 0 2 0.684 0 1 1 694.8772 1388.74712 1388.74704 0.05 4e-05 5032124.0 CID (Collision Induced Dissociation) MS2 6.944068 90 216.1 60.0 88.6613 28501 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 48.8 182.6 351.9 54.1 164.8 427.2 78.5 175.3 449.3 228.0 3.07 1 0.0003003 9.877e-05 False High Sequest HT (A2) Unambiguous GNAGGSNDDFTAPISATAK [K].gNAGGsNDDFTAPISATAk.[M] N-Term(TMT6plex); S6(Phospho); K19(TMT6plex) 1 P32578 P32578 0 3 0.2404 0 1 1 778.0463 2332.12434 2332.12224 0.9 0.0007 3540305.75 CID (Collision Induced Dissociation) MS2 1.348167 50 54.4 26.654 89.1089 28668 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 13.1 46.2 82.5 14.9 51.5 110.9 16.4 51.3 96.0 61.6 Excluded by Method 4.16 1 0.0003003 4.983e-05 False High Sequest HT (A2) Unambiguous SIFDTDDDD [K].sIFDtDDDD.[-] N-Term(TMT6plex); T5(Phospho) 1 P36054 P36054 0 2 0.5174 0 1 1 676.2607 1351.51412 1351.514 0.09 6e-05 1379092.375 CID (Collision Induced Dissociation) MS2 11.45858 70 100.5 60.0 90.2313 29090 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 35.5 81.8 158.1 37.1 84.8 188.2 39.4 84.3 190.9 104.5 3.17 1 0.0003003 9.885e-05 False High Sequest HT (A2) Unambiguous RSYSSPDITQAIQEEEK [K].rSYsSPDITQAIQEEEk.[R] N-Term(TMT6plex); S4(Phospho); K17(TMT6plex) 2 P40818 P40818; P40818-2 1 3 0.0289 0 1 1 840.42074 2519.24768 2519.24308 1.82 0.00153 831226.8125 CID (Collision Induced Dissociation) MS2 4.813459 70 229.5 60.0 90.1721 29068 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 231.0 248.0 186.0 240.2 202.4 225.2 265.7 250.2 221.0 225.0 7.61 1 0.0003003 1.163e-08 False High Sequest HT (A2) Unambiguous VQGEAVSNIQENTQTPTVQEESEEEEVDETGVEVK [K].vQGEAVSNIQENTQTPTVQEEsEEEEVDETGVEVk.[D] N-Term(TMT6plex); S22(Phospho); K35(TMT6plex) 3 Q13765 Q13765; E9PAV3; E9PAV3-2 0 4 0.0394 0 1 1 1100.52118 4399.06288 4399.06016 0.62 0.00068 480371.09375 CID (Collision Induced Dissociation) MS2 0.0 30 27.1 60.0 105.6806 34883 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 28.8 24.6 19.4 34.0 22.7 23.5 30.8 32.7 24.8 29.2 Excluded by Method 5.84 1 0.0003003 1.395e-05 False High Sequest HT (A2) Unambiguous QGSPDQVSPVSEMTSTSLYQDK [K].qGsPDQVsPVSEmTSTSLYQDk.[Q] N-Term(TMT6plex); S3(Phospho); S8(Phospho); M13(Oxidation); K22(TMT6plex) 1 P46821 P46821 0 3 0.2627 0 1 1 1006.78849 3018.35091 3018.34565 1.74 0.00175 301224.9375 CID (Collision Induced Dissociation) MS2 0.0 70 25.8 60.0 90.0311 29015 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 25.7 27.6 22.9 26.1 28.6 23.2 22.8 27.8 27.2 26.5 5.1 1 0.0003003 7.117e-06 False High Sequest HT (A2) Unambiguous EEEPILAPSVK [K].eEEPILAPsVk.[D] N-Term(TMT6plex); S9(Phospho); K11(TMT6plex) 1 P05750 P05750 0 2 0.5133 0 1 1 875.4754 1749.94351 1749.94399 -0.27 -0.00024 1602072.75 CID (Collision Induced Dissociation) MS2 0.0 50 164.2 60.0 89.9459 28982 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 40.1 129.9 253.8 45.9 139.0 310.1 60.3 149.0 347.0 166.5 Excluded by Method 4.15 1 0.0003003 0.0001179 False High Sequest HT (A2) Unambiguous GRAEGEWEDQEALDYFSDK [K].gRAEGEWEDQEALDYFsDk.[E] N-Term(TMT6plex); S17(Phospho); K19(TMT6plex) 3 Q9NYF8 Q9NYF8-3; Q9NYF8; Q9NYF8-2 1 3 0.1353 0 1 1 928.42456 2783.25912 2783.26019 -0.38 -0.00036 1077603.0 CID (Collision Induced Dissociation) MS2 33.10912 30 41.3 60.0 105.8715 34955 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 37.8 38.1 32.9 46.1 41.3 41.8 47.5 43.5 38.6 45.1 Excluded by Method 3.99 1 0.0003003 8.237e-05 False High Sequest HT (A2) Unambiguous ALSYASLDK [K].aLSYAsLDk.[E] N-Term(TMT6plex); S6(Phospho); K9(TMT6plex) 2 Q6R327 Q6R327; Q6R327-3 0 2 0.1327 0 1 1 753.40472 1505.80217 1505.80168 0.32 0.00024 3385730.0 CID (Collision Induced Dissociation) MS2 0.0 30 107.7 55.512 89.9163 28971 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 102.2 99.9 81.5 117.1 109.9 107.9 132.0 110.4 115.1 100.7 Excluded by Method 3.92 1 0.0003003 0.0005091 False High Sequest HT (A2) Unambiguous NAEEESESEAEEGD [K].nAEEEsESEAEEGD.[-] N-Term(TMT6plex); S6(Phospho) 4 Q7L1Q6 Q7L1Q6-2; Q7L1Q6-4; Q7L1Q6-3; Q7L1Q6 0 2 0.2158 0 1 1 917.34174 1833.67621 1833.67487 0.73 0.00067 256319.828125 CID (Collision Induced Dissociation) MS2 0.0 90 57.7 60.0 37.3821 9423 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 56.2 53.1 46.5 57.6 61.2 53.5 59.0 64.6 67.9 57.8 3.66 1 0.0003003 0.0005595 False High Sequest HT (A2) Unambiguous EYVPLDNAEQSTSSSQETK [K].eYVPLDNAEQSTSssQETk.[E] N-Term(TMT6plex); S14(Phospho); S15(Phospho); K19(TMT6plex) 1 P33322 P33322 0 3 0.0383 0 1 1 911.07631 2731.21438 2731.21529 -0.33 -0.0003 546256.1875 CID (Collision Induced Dissociation) MS2 9.382609 40 33.5 60.0 89.6908 28885 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 15.5 25.3 43.9 16.1 26.6 53.8 15.8 30.4 69.8 37.7 Excluded by Method 3.39 1 0.0003003 0.000555 False High Sequest HT (A2) Unambiguous YLEIESSPLK [K].yLEIESsPLk.[L] N-Term(TMT6plex); S7(Phospho); K10(TMT6plex) 1 P13382 P13382 0 3 0.1486 0 1 1 572.97901 1716.92248 1716.92253 -0.03 -2e-05 584895.4375 CID (Collision Induced Dissociation) MS2 8.151353 60 157.7 60.0 106.8917 35330 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 39.0 117.9 254.0 49.0 133.1 317.0 53.1 142.6 323.5 147.5 Excluded by Method 3.23 1 0.0003003 0.0002549 False High Sequest HT (A2) Unambiguous TNLNASAESNSVEWGPGK [K].tNLNASAESNsVEWGPGk.[S] N-Term(TMT6plex); S11(Phospho); K18(TMT6plex) 1 P53550 P53550 0 3 0.0204 0 1 1 800.39247 2399.16285 2399.16444 -0.66 -0.00053 600100.5 CID (Collision Induced Dissociation) MS2 35.14361 50 48.2 60.0 87.2497 27970 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 38.1 45.6 49.1 33.8 45.3 62.2 45.3 49.6 58.3 54.5 Excluded by Method 3.43 1 0.0003003 0.0001486 False High Sequest HT (A2) Unambiguous KKGSGEDDEEETETTPTSTVPVATIAQETLK [K].kkGSGEDDEEETETtPTSTVPVATIAQETLk.[V] N-Term(TMT6plex); K1(TMT6plex); K2(TMT6plex); T15(Phospho); K31(TMT6plex) 1 P12962 P12962 2 4 0.0251 0 1 1 1072.80881 4288.21339 4288.21425 -0.2 -0.00022 565752.4375 CID (Collision Induced Dissociation) MS2 0.0 30 31.6 60.0 107.0767 35397 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 11.2 19.1 45.7 13.1 29.8 53.1 12.9 34.6 63.9 32.6 Excluded by Method 3.98 1 0.0003003 0.0002292 False High Sequest HT (A2) Unambiguous LFEDDDSNEK [K].lFEDDDsNEk.[L] N-Term(TMT6plex); S7(Phospho); K10(TMT6plex) 1 O43719 O43719 0 3 0.4593 0 1 1 583.93774 1749.79866 1749.79845 0.12 7e-05 1346495.75 CID (Collision Induced Dissociation) MS2 0.0 60 238.2 60.0 86.6923 27766 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 246.7 232.8 191.6 285.2 219.9 217.5 284.8 244.3 233.4 226.2 Excluded by Method 4.42 1 0.0003003 3.059e-06 False High Sequest HT (A2) Unambiguous VSDDSESESGDK [K].vSDDsESESGDk.[E] N-Term(TMT6plex); S5(Phospho); K12(TMT6plex) 1 Q08723 Q08723 0 3 0.088 0 1 1 598.26799 1792.7894 1792.78901 0.22 0.00013 710290.75 CID (Collision Induced Dissociation) MS2 26.99221 50 34.9 60.0 44.6568 12046 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 22.6 30.4 47.2 17.4 32.1 58.4 21.1 34.8 52.8 32.1 Excluded by Method 3.75 1 0.0003003 0.0003914 False High Sequest HT (A2) Unambiguous RSIFASPESVTGK [K].rSIFAsPESVTGk.[V] N-Term(TMT6plex); S6(Phospho); K13(TMT6plex) 1 O75940 O75940 1 3 0.1739 0 1 1 639.68023 1917.02615 1917.0247 0.76 0.00048 1117980.25 CID (Collision Induced Dissociation) MS2 15.50926 40 122.9 60.0 87.9055 28218 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 117.5 125.2 108.6 118.0 112.3 127.1 132.6 132.8 121.9 132.5 Excluded by Method 4.37 1 0.0003003 5.216e-05 False High Sequest HT (A2) Unambiguous LFEDDDSNEK [K].lFEDDDsNEk.[L] N-Term(TMT6plex); S7(Phospho); K10(TMT6plex) 1 O43719 O43719 0 3 0.4786 0 1 1 583.93774 1749.79866 1749.79845 0.12 7e-05 1523322.75 CID (Collision Induced Dissociation) MS2 5.489312 60 100.4 60.0 87.8881 28211 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 100.1 94.9 89.0 113.4 93.7 86.2 116.2 106.2 101.8 102.6 Excluded by Method 3.74 1 0.0003003 2.4e-05 False High Sequest HT (A2) Unambiguous DTFEHDPSESIDEFNK [K].dTFEHDPSEsIDEFNk.[S] N-Term(TMT6plex); S10(Phospho); K16(TMT6plex) 4 Q9NYF8 Q9NYF8-3; Q9NYF8-4; Q9NYF8; Q9NYF8-2 0 3 0.1262 0 1 1 816.70599 2448.10341 2448.10084 1.05 0.00086 3752794.75 CID (Collision Induced Dissociation) MS2 3.738261 40 156.9 60.0 87.6644 28127 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 187.3 160.7 116.5 174.9 138.8 154.9 175.0 156.4 157.2 147.0 Excluded by Method 5.31 1 0.0003003 7.358e-06 False High Sequest HT (A2) Unambiguous VVDYSQFQESDDADEDYGRDSGPPTK [K].vVDYSQFQEsDDADEDYGRDSGPPTk.[K] N-Term(TMT6plex); S10(Phospho); K26(TMT6plex) 1 Q9H1E3 Q9H1E3 1 4 0.1306 0 1 1 865.38782 3458.52945 3458.53134 -0.55 -0.00047 1054125.375 CID (Collision Induced Dissociation) MS2 0.0 50 45.3 40.966 93.0549 30152 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 50.3 43.7 40.0 41.4 43.0 48.7 44.6 46.6 43.7 50.8 Excluded by Method 6.97 1 0.0003003 2.251e-05 False High Sequest HT (A2) Unambiguous EQRDRICSDEEEDEEK [K].eQRDRIcsDEEEDEEk.[G] N-Term(TMT6plex); C7(Carbamidomethyl); S8(Phospho); K16(TMT6plex) 2 Q9NPG3 Q9NPG3-2; Q9NPG3 2 4 0.3412 0 1 1 652.04265 2605.14876 2605.14893 -0.06 -4e-05 377987.8125 CID (Collision Induced Dissociation) MS2 27.68979 30 34.3 60.0 43.3228 11562 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 37.6 34.4 28.5 32.7 32.9 35.2 44.3 34.7 34.0 29.0 Excluded by Method 4.69 1 0.0003003 8.298e-05 False High Sequest HT (A2) Unambiguous GRAEGEWEDQEALDYFSDK [K].gRAEGEWEDQEALDYFsDk.[E] N-Term(TMT6plex); S17(Phospho); K19(TMT6plex) 3 Q9NYF8 Q9NYF8-3; Q9NYF8; Q9NYF8-2 1 3 0.1634 0 1 1 928.42505 2783.26059 2783.26019 0.14 0.00013 1275521.125 CID (Collision Induced Dissociation) MS2 0.0 40 65.7 60.0 104.4048 34414 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 65.6 68.1 48.7 63.7 57.3 63.4 76.3 69.5 72.5 72.0 Excluded by Method 5.08 1 0.0003003 2.839e-05 False High Sequest HT (A2) Unambiguous DVPPLSETEATPVPIK [K].dVPPLSETEAtPVPIk.[D] N-Term(TMT6plex); T11(Phospho); K16(TMT6plex) 2 P27816 P27816; P27816-6 0 3 0.3829 0 1 1 744.40534 2231.20148 2231.19764 1.72 0.00128 998233.6875 CID (Collision Induced Dissociation) MS2 13.80471 50 59.3 60.0 104.4188 34419 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 64.1 59.7 48.9 56.6 59.9 62.5 66.1 65.7 52.6 56.9 Excluded by Method 5.25 1 0.0003003 1.817e-06 False High Sequest HT (A2) Unambiguous VVDYSQFQESDDADEDYGRDSGPPTK [K].vVDYSQFQEsDDADEDYGRDSGPPTk.[K] N-Term(TMT6plex); S10(Phospho); K26(TMT6plex) 1 Q9H1E3 Q9H1E3 1 4 0.1287 0 1 1 865.38831 3458.53141 3458.53134 0.02 2e-05 2719577.5 CID (Collision Induced Dissociation) MS2 40.63329 70 70.0 26.219 91.5248 29577 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 74.6 79.4 58.0 70.0 58.2 73.4 79.6 72.1 68.4 65.9 8.47 1 0.0003003 3.708e-07 False High Sequest HT (A2) Unambiguous DTFEHDPSESIDEFNK [K].dTFEHDPsESIDEFNk.[S] N-Term(TMT6plex); S8(Phospho); K16(TMT6plex) 4 Q9NYF8 Q9NYF8-3; Q9NYF8-4; Q9NYF8; Q9NYF8-2 0 3 0.1239 0 1 1 816.70446 2448.09883 2448.10084 -0.82 -0.00067 1339856.25 CID (Collision Induced Dissociation) MS2 38.50557 80 40.8 60.0 91.4777 29560 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 37.9 36.5 39.0 38.7 39.1 45.3 39.2 40.0 52.5 39.3 5.65 1 0.0003003 1.259e-05 False High Sequest HT (A2) Unambiguous QKEDVEVVGGSDGEGAIGLSSDPK [K].qkEDVEVVGGsDGEGAIGLSSDPk.[S] N-Term(TMT6plex); K2(TMT6plex); S11(Phospho); K24(TMT6plex) 1 Q8NHZ8 Q8NHZ8 1 4 0.3976 0 1 1 785.90512 3140.59864 3140.59673 0.61 0.00048 774827.4375 CID (Collision Induced Dissociation) MS2 12.06052 40 49.8 58.508 91.4651 29555 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 52.1 45.4 37.8 54.5 49.3 50.5 53.1 57.2 44.6 53.3 Excluded by Method 4.93 1 0.0003003 0.0004914 False High Sequest HT (A2) Unambiguous RQSSTPSAPELGQQPDVNISEWK [K].rQSsTPSAPELGQQPDVNISEWk.[D] N-Term(TMT6plex); S4(Phospho); K23(TMT6plex) 4 Q93100 Q93100-2; Q93100; Q93100-3; Q93100-4 1 4 0.0108 0 1 1 773.89207 3092.54644 3092.54541 0.33 0.00026 1181583.75 CID (Collision Induced Dissociation) MS2 0.0 40 57.8 60.0 104.314 34381 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 58.3 55.0 47.9 57.6 58.6 63.3 63.5 64.6 55.5 53.8 Excluded by Method 5.55 1 0.0003003 1.437e-05 False High Sequest HT (A2) Unambiguous GGITTGDISEEETVDNSINTEEYDK [K].gGITTGDIsEEETVDNSINTEEYDk.[L] N-Term(TMT6plex); S9(Phospho); K25(TMT6plex) 1 Q06704 Q06704 0 3 0.0363 0 1 1 1085.50008 3254.48567 3254.48774 -0.64 -0.00069 1019375.75 CID (Collision Induced Dissociation) MS2 0.0 50 54.6 60.0 103.9202 34234 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 12.3 45.8 95.4 15.5 49.7 96.4 18.5 48.4 108.7 54.8 Excluded by Method 8.26 1 0.0003003 3.176e-09 False High Sequest HT (A2) Unambiguous KNPDEDEFLINSDDEM [K].kNPDEDEFLINsDDEm.[-] N-Term(TMT6plex); K1(TMT6plex); S12(Phospho); M16(Oxidation) 1 Q08412 Q08412 1 3 0.6864 0 1 1 822.36608 2465.08368 2465.08314 0.22 0.00018 2647641.5 CID (Collision Induced Dissociation) MS2 14.09218 30 252.4 35.753 92.9424 30110 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 64.7 207.0 372.1 77.8 204.4 476.7 89.7 252.5 506.2 273.0 Excluded by Method 4.56 1 0.0003003 0.0001743 False High Sequest HT (A2) Unambiguous EEIQETQTPTHSRK [K].eEIQETQtPTHSRk.[K] N-Term(TMT6plex); T8(Phospho); K14(TMT6plex) 1 Q9BYG3 Q9BYG3 1 4 0.0749 0 1 1 556.28634 2222.12353 2222.12185 0.76 0.00042 325424.53125 CID (Collision Induced Dissociation) MS2 25.22134 80 35.4 60.0 41.5973 10939 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 37.5 39.6 29.6 31.1 33.0 34.4 42.9 37.5 35.8 32.2 3.07 1 0.0003003 8.019e-05 False High Sequest HT (A2) Unambiguous SLDEIIGSNK [K].sLDEIIGSNk.[A] N-Term(TMT6plex); S1(Phospho); K10(TMT6plex) 1 Q12159 Q12159 0 3 0.2366 0 1 1 538.62303 1613.85453 1613.85517 -0.4 -0.00021 764286.375 CID (Collision Induced Dissociation) MS2 9.996563 90 23.3 60.0 103.9753 34255 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 9.5 19.3 32.0 3.7 20.3 42.5 8.8 24.3 46.2 26.8 2.79 1 0.0003003 0.0003576 False High Sequest HT (A2) Unambiguous LAAVPAAGPASAGGAAAASGDAAAEEEK [K].lAAVPAAGPAsAGGAAAASGDAAAEEEk.[E] N-Term(TMT6plex); S11(Phospho); K28(TMT6plex) 1 P05319 P05319 0 3 0.3684 0 1 1 973.82228 2919.45228 2919.45012 0.74 0.00072 564209.5 CID (Collision Induced Dissociation) MS2 0.0 40 23.1 60.0 92.5938 29981 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 15.7 19.3 29.5 14.2 18.1 37.8 12.3 23.5 41.7 19.3 Excluded by Method 3.23 1 0.0003003 3.894e-05 False High Sequest HT (A2) Unambiguous LCAGIMITASHNPK [K].lcAGIMITAsHNPk.[Q] N-Term(TMT6plex); C2(Carbamidomethyl); S10(Phospho); K14(TMT6plex) 2 Q96G03 Q96G03-2; Q96G03 0 3 0.2642 0 1 1 684.3578 2051.05884 2051.05833 0.25 0.00017 504985.59375 CID (Collision Induced Dissociation) MS2 38.39001 70 63.1 60.0 92.3226 29881 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 67.6 63.5 54.4 60.3 62.0 63.9 70.7 67.2 64.9 56.1 3.52 1 0.0003003 0.0002404 False High Sequest HT (A2) Unambiguous LIDENATENGLAGSPK [K].lIDENATENGLAGsPk.[D] N-Term(TMT6plex); S14(Phospho); K16(TMT6plex) 1 P31374 P31374 0 3 0.5137 0 1 1 723.04013 2167.10584 2167.1048 0.48 0.00035 2068586.125 CID (Collision Induced Dissociation) MS2 6.06137 80 98.9 36.417 92.0928 29793 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 24.2 86.1 137.9 37.9 80.2 174.8 34.8 93.3 212.6 107.6 4.73 1 0.0003003 8.595e-07 False High Sequest HT (A2) Unambiguous EQNVAFSPLK [K].eQNVAFsPLk.[R] N-Term(TMT6plex); S7(Phospho); K10(TMT6plex) 1 Q12882 Q12882 0 3 0.2216 0 1 1 557.63528 1670.89128 1670.89189 -0.37 -0.00021 1399930.125 CID (Collision Induced Dissociation) MS2 6.712167 70 83.5 60.0 91.9985 29758 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 83.6 89.4 60.0 90.9 75.3 81.1 95.7 91.3 85.3 82.3 5.01 1 0.0003003 5.702e-05 False High Sequest HT (A2) Unambiguous KSPSEARQDVDLCLVSSCEYK [K].ksPSEARQDVDLcLVSScEYk.[H] N-Term(TMT6plex); K1(TMT6plex); S2(Phospho); C13(Carbamidomethyl); C18(Carbamidomethyl); K21(TMT6plex) 1 P46821 P46821 2 4 0.1366 0 1 1 810.40753 3238.6083 3238.60923 -0.29 -0.00023 1728633.25 CID (Collision Induced Dissociation) MS2 9.119281 40 54.9 54.633 91.4381 29544 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 49.9 55.4 39.7 57.4 53.4 62.5 58.8 59.2 54.8 57.5 Excluded by Method 4.32 1 0.0003003 2.815e-05 False High Sequest HT (A2) Unambiguous TMFAQVESDDEEAK [K].tMFAQVEsDDEEAk.[N] N-Term(TMT6plex); S8(Phospho); K14(TMT6plex) 1 Q9NW82 Q9NW82 0 3 0.3764 0 1 1 713.33079 2137.97782 2137.97649 0.62 0.00044 5381242.5 CID (Collision Induced Dissociation) MS2 7.201939 50 118.6 27.168 91.4364 29543 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 128.3 119.1 75.7 132.2 110.5 111.8 148.6 125.2 108.3 125.9 Excluded by Method 6.19 1 0.0003003 2.152e-06 False High Sequest HT (A2) Unambiguous GILAADESTGSIAK [K].gILAADESTGsIAk.[R] N-Term(TMT6plex); S11(Phospho); K14(TMT6plex) 2 P04075 P04075; P04075-2 0 3 0.1083 0 1 1 624.33613 1870.99385 1870.99273 0.6 0.00037 2411611.0 CID (Collision Induced Dissociation) MS2 1.078268 10 132.1 35.754 90.7195 29272 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 127.4 125.0 111.6 147.5 119.2 126.6 149.8 140.6 132.8 140.9 Excluded by Method 3.51 1 0.0003003 6.249e-05 False High Sequest HT (A2) Unambiguous GLINDPDVNNTFNINK [K].gLINDPDVNNTFNINk.[G] N-Term(TMT6plex); K16(TMT6plex) 1 P32449 P32449 0 3 0.5013 0 1 1 749.41095 2246.21831 2246.21812 0.08 6e-05 1127232.75 CID (Collision Induced Dissociation) MS2 11.03666 40 112.9 31.12 105.3989 34783 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 26.6 95.2 178.2 35.4 89.4 215.9 41.8 107.2 219.2 119.7 Excluded by Method 3.89 1 0.0003003 0.0003759 False High Sequest HT (A2) Unambiguous DEGIGSPDIWEDEK [K].dEGIGsPDIWEDEk.[A] N-Term(TMT6plex); S6(Phospho); K14(TMT6plex) 1 Q01664 Q01664 0 3 0.3508 0 1 1 710.00169 2127.99053 2127.98877 0.83 0.00059 1269839.0 CID (Collision Induced Dissociation) MS2 0.0 70 130.0 53.319 105.4758 34811 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 128.0 122.8 112.2 155.9 113.0 116.9 156.0 132.4 135.9 127.0 4.96 1 0.0003003 6.621e-05 False High Sequest HT (A2) Unambiguous VVDYSQFQESDDADEDYGRDSGPPTK [K].vVDYSQFQEsDDADEDYGRDSGPPTk.[K] N-Term(TMT6plex); S10(Phospho); K26(TMT6plex) 1 Q9H1E3 Q9H1E3 1 3 0.1012 0 1 1 1153.51539 3458.5316 3458.53134 0.08 9e-05 2766035.0 CID (Collision Induced Dissociation) MS2 0.0 70 64.1 51.733 90.3761 29142 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 56.9 73.0 60.1 58.5 58.3 64.1 61.7 72.6 66.1 70.1 5.73 1 0.0003003 5.814e-06 False High Sequest HT (A2) Unambiguous LWNLQNANNK [K].lWNLQNANNk.[S] N-Term(TMT6plex); K10(TMT6plex) 1 P16649 P16649 0 3 0.3846 0 1 1 558.32271 1672.95359 1672.95351 0.05 3e-05 1500189.125 CID (Collision Induced Dissociation) MS2 6.128327 40 49.2 47.925 90.8994 29338 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 20.4 51.5 61.8 23.7 37.2 80.0 30.9 47.7 88.4 50.4 Excluded by Method 3.38 1 0.0003003 0.000557 False High Sequest HT (A2) Unambiguous EYVPLDNAEQSTSSSQETK [K].eYVPLDNAEQStSSsQETk.[E] N-Term(TMT6plex); T12(Phospho); S15(Phospho); K19(TMT6plex) 1 P33322 P33322 0 3 0.0263 0 1 1 911.07607 2731.21364 2731.21529 -0.6 -0.00055 754643.0625 CID (Collision Induced Dissociation) MS2 4.66384 70 70.5 60.0 90.9171 29345 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 25.0 59.4 101.8 31.0 56.2 125.0 27.5 62.4 139.3 77.7 4.19 1 0.0003003 5.943e-05 False High Sequest HT (A2) Unambiguous EFTGSQDDGEDEYDLDK [K].eFTGsQDDGEDEYDLDk.[L] N-Term(TMT6plex); S5(Phospho); K17(TMT6plex) 1 P53725 P53725 0 3 0.0114 0 1 1 834.35959 2501.06423 2501.06451 -0.11 -9e-05 3596579.0 CID (Collision Induced Dissociation) MS2 6.991713 70 48.3 34.473 91.25 29472 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 11.9 43.8 67.7 14.2 43.9 85.9 15.6 40.1 102.4 57.7 5.28 1 0.0003003 2.776e-06 False High Sequest HT (A2) Unambiguous VSAEAEVAPVSPEVTQEVVEEHCASPEDK [K].vSAEAEVAPVsPEVTQEVVEEHcAsPEDk.[T] N-Term(TMT6plex); S11(Phospho); C23(Carbamidomethyl); S25(Phospho); K29(TMT6plex) 1 P46821 P46821 0 4 0.1125 0 1 1 935.93163 3740.70469 3740.70556 -0.23 -0.00022 784166.0625 CID (Collision Induced Dissociation) MS2 0.0 50 108.0 60.0 104.9745 34625 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 120.4 99.0 81.2 126.9 95.1 102.0 128.1 109.9 106.0 111.4 Excluded by Method 5.51 1 0.0003003 1.182e-05 False High Sequest HT (A2) Unambiguous DSVDDNENSDDGLDIPK [K].dSVDDNENsDDGLDIPk.[N] N-Term(TMT6plex); S9(Phospho); K17(TMT6plex) 1 Q06631 Q06631 0 3 0.2406 0 1 1 796.02785 2386.06899 2386.06993 -0.39 -0.00031 835109.625 CID (Collision Induced Dissociation) MS2 8.344373 20 184.7 60.0 91.0504 29397 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 46.6 154.1 282.9 59.2 155.5 341.6 75.7 188.1 358.2 185.2 Excluded by Method 5.07 1 0.0003003 9.509e-07 False High Sequest HT (A2) Unambiguous FACQIDIPNEENNNASK [K].fAcQIDIPNEENNNASk.[D] N-Term(TMT6plex); C3(Carbamidomethyl); K17(TMT6plex) 1 P06105 P06105 0 3 0.4906 0 1 1 808.07392 2422.2072 2422.20729 -0.04 -3e-05 2014795.625 CID (Collision Induced Dissociation) MS2 1.891529 50 97.3 43.73 91.0056 29379 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 22.1 74.2 144.8 36.5 87.1 188.0 31.1 94.3 191.6 103.0 Excluded by Method 3.73 1 0.0003003 6.739e-06 False High Sequest HT (A2) Unambiguous IELSDDSDVEVHPNVDK [K].iELSDDsDVEVHPNVDk.[K] N-Term(TMT6plex); S7(Phospho); K17(TMT6plex) 1 P06101 P06101 0 3 0.0495 0 1 1 817.06823 2449.19014 2449.18999 0.06 5e-05 3624646.25 CID (Collision Induced Dissociation) MS2 7.092176 40 49.9 34.831 90.9919 29373 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 16.6 46.7 69.9 22.5 44.0 83.8 22.4 45.5 91.9 55.2 Excluded by Method 6.46 1 0.0003003 8.485e-07 False High Sequest HT (A2) Unambiguous DSGSDEDFLMEDDDDSDYGSSK [K].dSGsDEDFLmEDDDDSDYGSSk.[K] N-Term(TMT6plex); S4(Phospho); M10(Oxidation); K22(TMT6plex) 2 Q9H1E3 Q9H1E3; Q9H1E3-2 0 3 0.0259 0 1 1 995.05791 2983.15918 2983.16 -0.28 -0.00028 303518.0625 CID (Collision Induced Dissociation) MS2 37.41417 60 5.5 60.0 90.9628 29362 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 3.1 6.0 6.4 3.4 7.4 7.2 6.4 3.7 8.2 3.6 Excluded by Method 3.48 1 0.0003003 4.578e-05 False High Sequest HT (A2) Unambiguous AEDGATPSPSNETPK [K].aEDGATPSPSNEtPk.[K] N-Term(TMT6plex); T13(Phospho); K15(TMT6plex) 1 P29966 P29966 0 3 0.1688 0 1 1 680.32954 2038.97408 2038.97345 0.31 0.00021 956801.375 CID (Collision Induced Dissociation) MS2 27.66442 60 127.9 60.0 49.2266 13724 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 131.0 123.8 100.9 144.2 121.1 118.0 145.9 130.8 137.3 125.9 Excluded by Method 3.85 1 0.0003003 1.034e-05 False High Sequest HT (A2) Unambiguous NIEDVIAQGIGK [K].nIEDVIAQGIGk.[L] N-Term(TMT6plex); K12(TMT6plex) 1 P05387 P05387 0 3 0.5077 0 1 1 572.34168 1715.01049 1715.01036 0.08 5e-05 1941657.625 CID (Collision Induced Dissociation) MS2 1.923638 70 178.2 60.0 118.4821 39639 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 177.6 184.1 146.1 172.9 162.4 177.7 208.0 211.1 170.3 171.4 3.9 1 0.0003003 3.021e-05 False High Sequest HT (A2) Unambiguous EMLASDDEEDVSSK [K].emLAsDDEEDVSSk.[V] N-Term(TMT6plex); M2(Oxidation); S5(Phospho); K14(TMT6plex) 4 Q0ZGT2 Q0ZGT2-2; Q0ZGT2-4; Q0ZGT2; Q0ZGT2-3 0 2 0.5116 0 1 1 1054.97069 2108.9341 2108.93468 -0.28 -0.00029 688706.0625 CID (Collision Induced Dissociation) MS2 0.0 20 115.4 60.0 66.0689 19996 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 122.1 121.0 93.7 125.2 102.0 105.2 128.8 132.4 116.6 107.4 Excluded by Method 3.87 1 0.0003003 2.316e-05 False High Sequest HT (A2) Unambiguous FSPGAPGGSGSQPNQK [K].fsPGAPGGSGSQPNQk.[L] N-Term(TMT6plex); S2(Phospho); K16(TMT6plex) 2 Q15942 Q15942; Q15942-2 0 3 0.2301 0 1 1 685.34168 2054.0105 2054.01084 -0.17 -0.00011 15605204.0 CID (Collision Induced Dissociation) MS2 0.0 50 297.8 8.311 66.2036 20048 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 296.0 274.1 229.3 310.4 291.4 307.3 331.7 324.5 303.3 309.8 Excluded by Method 4.65 1 0.0003003 1.191e-06 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVSsDEEF.[-] N-Term(TMT6plex); S9(Phospho) 1 P16521 P16521 0 2 0.0386 0 1 1 885.3712 1769.73512 1769.73562 -0.29 -0.00025 1437560.5 CID (Collision Induced Dissociation) MS2 8.391274 50 90.7 60.0 118.339 39586 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 24.9 83.1 148.0 24.6 72.7 173.6 30.7 86.9 178.4 84.5 Excluded by Method 4.66 1 0.0003003 4.579e-07 False High Sequest HT (A2) Unambiguous GEPAAAAAPEAGASPVEK [K].gEPAAAAAPEAGAsPVEk.[E] N-Term(TMT6plex); S14(Phospho); K18(TMT6plex) 1 P29966 P29966 0 4 0.463 0 1 1 541.02934 2161.09552 2161.09424 0.59 0.00032 2823853.25 CID (Collision Induced Dissociation) MS2 3.419219 50 134.3 48.06 66.5677 20183 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 135.2 137.4 105.2 132.3 136.3 137.0 152.8 148.3 125.6 132.9 Excluded by Method 3.65 1 0.0003003 0.0003768 False High Sequest HT (A2) Unambiguous GEAAAERPGEAAVASSPSK [K].gEAAAERPGEAAVAsSPSk.[A] N-Term(TMT6plex); S15(Phospho); K19(TMT6plex) 1 P29966 P29966 0 3 0.0082 0 1 1 775.06107 2323.16866 2323.16953 -0.37 -0.00029 648988.3125 CID (Collision Induced Dissociation) MS2 57.13589 40 101.9 60.0 51.3028 14487 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 108.7 101.4 67.5 108.2 91.8 91.0 128.4 108.0 101.0 113.5 Excluded by Method 3.65 1 0.0003003 0.0001212 False High Sequest HT (A2) Unambiguous TFVLTPELSPGK [K].tFVLTPELsPGk.[L] N-Term(TMT6plex); S9(Phospho); K12(TMT6plex) 1 Q96DY7 Q96DY7 0 3 0.5771 0 1 1 609.67361 1827.00628 1827.00692 -0.35 -0.00021 1167836.875 CID (Collision Induced Dissociation) MS2 6.665551 70 113.2 60.0 118.1726 39523 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 109.1 115.9 90.1 126.0 107.6 111.8 119.7 123.2 119.6 109.5 5.06 1 0.0003003 2.021e-05 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVssDEEF.[-] N-Term(TMT6plex); S8(Phospho); S9(Phospho) 1 P16521 P16521 0 2 0.1491 0 1 1 925.35577 1849.70427 1849.70195 1.25 0.00116 2691181.25 CID (Collision Induced Dissociation) MS2 14.57239 40 155.7 51.934 118.1539 39516 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 39.9 126.7 264.2 47.9 119.8 316.1 53.0 138.8 300.3 150.1 Excluded by Method 4.56 1 0.0003003 0.0002673 False High Sequest HT (A2) Unambiguous EKTPSPKEEDEEPESPPEK [K].ektPsPkEEDEEPEsPPEk.[K] N-Term(TMT6plex); K2(TMT6plex); T3(Phospho); S5(Phospho); K7(TMT6plex); S15(Phospho); K19(TMT6plex) 2 Q9H1E3 Q9H1E3; Q9H1E3-2 2 4 0.3977 0 1 1 835.39365 3338.55277 3338.5541 -0.4 -0.00033 548271.1875 CID (Collision Induced Dissociation) MS2 10.17416 50 36.2 60.0 66.3731 20111 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 41.6 28.2 21.5 54.0 34.4 27.7 44.6 35.2 43.1 31.8 Excluded by Method 6.06 1 0.0003003 0.0003417 False High Sequest HT (A2) Unambiguous EYDVDMEDSDDEK [K].eYDVDmEDsDDEk.[D] N-Term(TMT6plex); M6(Oxidation); S9(Phospho); K13(TMT6plex) 1 P53313 P53313 0 3 0.5584 0 1 1 715.29368 2143.86649 2143.86666 -0.08 -6e-05 703312.3125 CID (Collision Induced Dissociation) MS2 0.0 60 92.3 60.0 63.2051 18932 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 23.6 77.9 125.6 35.7 77.0 171.9 36.3 90.4 188.7 96.0 Excluded by Method 5.39 1 0.0003003 9.683e-07 False High Sequest HT (A2) Unambiguous HYNESSELSQNNTEIDK [K].hYNEsSELSQNNTEIDk.[L] N-Term(TMT6plex); S5(Phospho); K17(TMT6plex) 1 P38853 P38853 0 3 0.0269 0 1 1 849.40004 2546.18558 2546.18121 1.72 0.00146 316328.0625 CID (Collision Induced Dissociation) MS2 0.0 70 15.3 60.0 64.7826 19523 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 1.6 13.5 21.4 3.2 11.1 33.7 9.0 12.9 29.3 17.0 5.21 1 0.0003003 1.215e-05 False High Sequest HT (A2) Unambiguous AEDGATPSPSNETPK [K].aEDGAtPSPSNEtPk.[K] N-Term(TMT6plex); T6(Phospho); T13(Phospho); K15(TMT6plex) 1 P29966 P29966 0 3 0.32 0 1 1 706.98516 2118.94091 2118.93978 0.53 0.00038 2637638.25 CID (Collision Induced Dissociation) MS2 14.05209 30 116.8 60.0 51.784 14670 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 113.1 117.6 92.5 136.0 107.8 112.5 132.4 116.9 123.9 115.3 Excluded by Method 4.5 1 0.0003003 0.0001122 False High Sequest HT (A2) Unambiguous WDGSEEDEDNSK [K].wDGsEEDEDNSk.[K] N-Term(TMT6plex); S4(Phospho); K12(TMT6plex) 1 O60841 O60841 0 2 0.4289 0 1 1 974.91505 1948.82282 1948.82137 0.75 0.00073 704850.6875 CID (Collision Induced Dissociation) MS2 0.0 40 54.1 60.0 65.213 19680 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 55.1 49.6 42.5 64.6 51.0 45.8 67.0 53.9 54.5 57.0 Excluded by Method 4.22 1 0.0003003 1.65e-05 False High Sequest HT (A2) Unambiguous TPSADSLPLSNSSTDLTISDNIK [K].tPSADSLPLSNsSTDLTISDNIk.[E] N-Term(TMT6plex); S12(Phospho); K23(TMT6plex) 1 P32525 P32525 0 3 0.0091 0 1 1 972.16212 2914.47181 2914.46985 0.67 0.00065 1424967.625 CID (Collision Induced Dissociation) MS2 2.836513 70 151.0 56.222 118.6993 39719 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 36.6 118.7 231.6 47.9 134.4 288.3 54.3 135.8 301.4 161.0 5.47 1 0.0003003 2.49e-07 False High Sequest HT (A2) Unambiguous AEASGEAAEEAEDEE [K].aEASGEAAEEAEDEE.[-] N-Term(TMT6plex) 1 P05755 P05755 0 2 0.8448 0 1 1 883.37633 1765.74538 1765.74493 0.25 0.00022 3467568.0 CID (Collision Induced Dissociation) MS2 0.0 100 196.1 60.0 51.7895 14672 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 43.1 163.0 317.3 65.4 154.3 381.7 63.0 181.5 378.2 213.6 5.22 1 0.0003003 2.146e-09 False High Sequest HT (A2) Unambiguous YNLDASEEEDSNKK [K].yNLDAsEEEDSNkk.[K] N-Term(TMT6plex); S6(Phospho); K13(TMT6plex); K14(TMT6plex) 2 O95218 O95218-2; O95218 1 4 0.2315 0 1 1 603.05063 2409.18071 2409.17898 0.72 0.00043 375360.21875 CID (Collision Induced Dissociation) MS2 0.0 30 201.3 60.0 68.5686 20943 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 190.6 191.7 161.5 246.1 190.0 190.4 223.0 219.0 201.9 198.6 Excluded by Method 5.14 1 0.0003003 8.009e-07 False High Sequest HT (A2) Unambiguous FPEFCSSPSPPVEVK [K].fPEFcSSPsPPVEVk.[I] N-Term(TMT6plex); C5(Carbamidomethyl); S9(Phospho); K15(TMT6plex) 4 Q68E01 Q68E01; Q68E01-4; Q68E01-2; Q68E01-3 0 3 0.1607 0 1 1 749.03846 2245.10081 2245.10163 -0.36 -0.00027 711100.25 CID (Collision Induced Dissociation) MS2 22.97381 40 114.0 60.0 117.7005 39346 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 111.5 114.4 109.0 114.1 101.4 112.3 122.7 124.6 115.7 114.1 Excluded by Method 4.73 1 0.0003003 2.712e-06 False High Sequest HT (A2) Unambiguous SVESTSPEPSK [K].sVESTsPEPSk.[I] N-Term(TMT6plex); S6(Phospho); K11(TMT6plex) 10 P18583 P18583; P18583-4; P18583-6; P18583-5; P18583-10; P18583-9; P18583-2; P18583-3; P18583-7; P18583-8 0 2 0.072 0 1 1 843.4241 1685.84092 1685.83992 0.59 0.0005 677354.625 CID (Collision Induced Dissociation) MS2 0.0 30 315.9 60.0 50.377 14141 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 329.3 296.6 267.3 317.5 287.4 315.0 365.0 334.5 317.1 328.9 Excluded by Method 3.47 1 0.0003003 3.728e-05 False High Sequest HT (A2) Unambiguous IMATPEQVGK [K].imAtPEQVGk.[M] N-Term(TMT6plex); M2(Oxidation); T4(Phospho); K10(TMT6plex) 2 P12956 P12956; P12956-2 0 2 0.4817 0 1 1 814.43084 1627.85441 1627.85307 0.82 0.00067 721712.3125 CID (Collision Induced Dissociation) MS2 0.0 30 120.9 60.0 69.1298 21156 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 120.8 115.0 111.5 117.6 126.3 116.6 141.9 132.6 115.7 111.4 Excluded by Method 3.55 1 0.0003003 0.0005489 False High Sequest HT (A2) Unambiguous LFPGSPAIYK [K].lFPGsPAIYk.[L] N-Term(TMT6plex); S5(Phospho); K10(TMT6plex) 9 P49792 P0DJD0; P49792; Q99666; Q7Z3J3-2; P0DJD1; Q99666-2; O14715; A6NKT7; Q7Z3J3 0 2 0.2082 0 1 1 815.9544 1630.90152 1630.901 0.32 0.00026 902564.9375 CID (Collision Induced Dissociation) MS2 44.07904 50 72.5 60.0 117.6374 39322 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 77.2 78.3 68.7 73.7 64.4 72.7 77.7 73.2 67.7 71.2 Excluded by Method 3.89 1 0.0003003 0.0001203 False High Sequest HT (A2) Unambiguous VVADDLVLVTPK [K].vVADDLVLVtPk.[K] N-Term(TMT6plex); T10(Phospho); K12(TMT6plex) 1 P38934 P38934 0 3 0.2592 0 1 1 603.01749 1807.03792 1807.03822 -0.17 -0.0001 597127.25 CID (Collision Induced Dissociation) MS2 0.0 40 74.7 60.0 117.3819 39227 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 19.8 62.5 114.8 29.1 54.2 134.4 34.1 67.3 151.6 79.3 Excluded by Method 4.36 1 0.0003003 0.0002018 False High Sequest HT (A2) Unambiguous VNNEEAISEDDGIEEEHIHSEK [K].vNNEEAIsEDDGIEEEHIHSEk.[S] N-Term(TMT6plex); S8(Phospho); K22(TMT6plex) 1 P39993 P39993 0 4 0.432 0 1 1 766.10619 3061.40292 3061.40395 -0.34 -0.00026 686828.8125 CID (Collision Induced Dissociation) MS2 17.68768 60 75.7 60.0 69.953 21471 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 23.8 55.7 113.2 23.3 63.2 149.1 32.5 72.3 146.5 77.4 Excluded by Method 6.62 1 0.0003003 1.002e-05 False High Sequest HT (A2) Unambiguous EVEDKESEGEEEDEDEDLSK [K].eVEDkEsEGEEEDEDEDLSk.[Y] N-Term(TMT6plex); K5(TMT6plex); S7(Phospho); K20(TMT6plex) 2 O95218 O95218-2; O95218 1 4 0.4044 0 1 1 777.60361 3107.39262 3107.392 0.2 0.00015 5006706.0 CID (Collision Induced Dissociation) MS2 0.0 20 108.7 33.358 70.035 21501 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 107.1 104.0 79.6 144.7 97.2 93.4 135.0 110.2 105.0 111.1 Excluded by Method 4.13 1 0.0003003 0.0003104 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVssDEEF.[-] N-Term(TMT6plex); S8(Phospho); S9(Phospho) 1 P16521 P16521 0 2 0.1235 0 1 1 925.35443 1849.70159 1849.70195 -0.2 -0.00018 1308791.25 CID (Collision Induced Dissociation) MS2 13.84083 30 106.0 60.0 117.0629 39106 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 24.8 82.7 168.8 33.4 94.0 196.0 38.0 99.4 215.2 107.5 Excluded by Method 3.32 1 0.0003003 0.0005049 False High Sequest HT (A2) Unambiguous VQGEAVSNIQENTQTPTVQEESEEEEVDETGVEVK [K].vQGEAVSNIQENTQTPtVQEESEEEEVDETGVEVk.[D] N-Term(TMT6plex); T17(Phospho); K35(TMT6plex) 3 Q13765 Q13765; E9PAV3; E9PAV3-2 0 4 0.038 0 1 1 1100.52118 4399.06288 4399.06016 0.62 0.00068 757501.125 CID (Collision Induced Dissociation) MS2 0.0 50 15.8 60.0 116.9895 39078 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 18.2 15.9 15.5 13.6 11.8 15.3 19.5 11.9 19.9 16.0 Excluded by Method 4.47 1 0.0003003 0.0001159 False High Sequest HT (A2) Unambiguous TVEDEDQDSEEEK [K].tVEDEDQDsEEEk.[D] N-Term(TMT6plex); S9(Phospho); K13(TMT6plex) 4 Q9Y5T5 Q9Y5T5-2; Q9Y5T5-3; Q9Y5T5-4; Q9Y5T5 0 3 0.4521 0 1 1 697.63971 2090.90457 2090.90549 -0.44 -0.00031 1416743.625 CID (Collision Induced Dissociation) MS2 9.307308 70 106.6 60.0 50.1373 14054 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 102.7 111.7 72.7 124.1 97.1 107.8 124.9 115.4 111.0 98.2 5.11 1 0.0003003 4.804e-06 False High Sequest HT (A2) Unambiguous AYEPDLSDADFEGIEASPK [K].aYEPDLsDADFEGIEASPk.[L] N-Term(TMT6plex); S7(Phospho); K19(TMT6plex) 1 P49687 P49687 0 3 0.1135 0 1 1 864.7439 2592.21715 2592.21587 0.49 0.00043 341482.1875 CID (Collision Induced Dissociation) MS2 0.0 50 47.9 60.0 117.7877 39379 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 13.7 41.5 72.6 12.2 34.6 90.2 20.4 45.0 101.7 47.6 Excluded by Method 4.58 1 0.0003003 0.0001693 False High Sequest HT (A2) Unambiguous ESDQTLAALLSPK [K].eSDQTLAALLsPk.[E] N-Term(TMT6plex); S11(Phospho); K13(TMT6plex) 9 P18583 P18583; P18583-4; P18583-6; P18583-5; P18583-10; P18583-9; P18583-2; P18583-3; P18583-7 0 3 0.4623 0 1 1 637.67981 1911.02487 1911.02403 0.44 0.00028 3991780.5 CID (Collision Induced Dissociation) MS2 5.405743 40 304.6 36.239 118.0653 39482 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 323.3 311.3 238.0 330.0 273.4 286.6 369.1 316.7 299.5 297.8 Excluded by Method 4.11 1 0.0003003 3.38e-05 False High Sequest HT (A2) Unambiguous GADFLVTEVENGGSLGSK [K].gADFLVTEVENGGSLGSk.[K] N-Term(TMT6plex); K18(TMT6plex) 3 P14618 P14618-2; P14618-3; P14618 0 3 0.5501 0 1 1 746.73926 2238.20324 2238.2018 0.64 0.00048 740996.625 CID (Collision Induced Dissociation) MS2 9.564835 80 86.4 60.0 118.0247 39468 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 79.7 87.8 67.7 87.2 79.5 103.2 90.9 93.7 87.7 86.5 4.09 1 0.0003003 2.699e-06 False High Sequest HT (A2) Unambiguous AGLDNVDAESK [K].aGLDNVDAEsk.[E] N-Term(TMT6plex); S10(Phospho); K11(TMT6plex) 1 P09064 P09064 0 2 0.484 0 1 1 828.91624 1656.8252 1656.8246 0.36 0.0003 1817425.375 CID (Collision Induced Dissociation) MS2 0.0 50 222.4 60.0 67.342 20478 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 52.3 189.4 347.7 66.3 183.7 418.6 72.5 219.4 468.6 205.1 Excluded by Method 4.38 1 0.0003003 6.083e-06 False High Sequest HT (A2) Unambiguous NIGRDTPTSAGPNSFNK [K].nIGRDtPTSAGPNSFNk.[G] N-Term(TMT6plex); T6(Phospho); K17(TMT6plex) 2 Q8WW12 Q8WW12; Q8WW12-3 1 3 0.1774 0 1 1 772.05802 2314.15952 2314.1593 0.1 7e-05 1378460.875 CID (Collision Induced Dissociation) MS2 8.816615 40 91.9 60.0 67.3804 20492 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 81.6 94.5 70.8 90.9 88.3 95.4 105.9 105.0 89.9 97.0 Excluded by Method 5.3 1 0.0003003 4.492e-07 False High Sequest HT (A2) Unambiguous EVSEEENTEPEVQEEK [K].eVsEEENtEPEVQEEk.[E] N-Term(TMT6plex); S3(Phospho); T8(Phospho); K16(TMT6plex) 1 P38249 P38249 0 3 0.5139 0 1 1 841.69919 2523.083 2523.08288 0.05 4e-05 323353.65625 CID (Collision Induced Dissociation) MS2 0.0 40 47.8 60.0 67.6365 20588 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 14.9 35.0 73.3 15.2 41.8 83.8 18.4 48.5 98.4 49.1 Excluded by Method 5.04 1 0.0003003 2.805e-05 False High Sequest HT (A2) Unambiguous VSGTLDTPEK [K].vSGTLDtPEk.[T] N-Term(TMT6plex); T7(Phospho); K10(TMT6plex) 1 Q9BYG3 Q9BYG3 0 2 0.1615 0 1 1 792.91756 1584.82784 1584.82863 -0.49 -0.00039 750499.375 CID (Collision Induced Dissociation) MS2 37.43496 30 192.7 60.0 67.7306 20624 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 195.8 192.4 159.9 218.5 177.6 175.1 214.7 194.3 202.8 196.0 Excluded by Method 3.84 1 0.0003003 2.346e-05 False High Sequest HT (A2) Unambiguous DTSATSQSVNGSPQAEQPSLESTSK [K].dTSATSQSVNGsPQAEQPSLESTSk.[E] N-Term(TMT6plex); S12(Phospho); K25(TMT6plex) 3 Q86WB0 Q86WB0-2; Q86WB0-3; Q86WB0 0 3 0.1796 0 1 1 1025.4905 3074.45693 3074.45672 0.07 7e-05 952673.3125 CID (Collision Induced Dissociation) MS2 0.0 70 51.0 60.0 67.7331 20625 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 52.1 45.0 35.2 54.1 49.3 51.8 56.0 59.9 46.7 60.0 6.85 1 0.0003003 1.49e-07 False High Sequest HT (A2) Unambiguous HPNYSPPGSPIEK [K].hPNySPPGsPIEk.[Y] N-Term(TMT6plex); Y4(Phospho); S9(Phospho); K13(TMT6plex) 3 Q9UHF7 Q9UHF7-3; Q9UHF7-2; Q9UHF7 0 3 0.0135 0 1 1 680.99153 2040.96004 2040.95973 0.15 0.0001 641741.8125 CID (Collision Induced Dissociation) MS2 16.80746 20 185.4 60.0 68.3574 20861 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 186.4 199.5 147.8 183.4 188.0 184.4 212.9 185.3 199.1 167.1 Excluded by Method 5.17 1 0.0003003 0.0003612 False High Sequest HT (A2) Unambiguous SVGFGLVYNSVAEAK [K].sVGFGLVYNSVAEAk.[K] N-Term(TMT6plex); K15(TMT6plex) 1 P0CX31 P0CX31 0 3 0.4912 0 1 1 667.0466 1999.12524 1999.12645 -0.6 -0.0004 395250.25 CID (Collision Induced Dissociation) MS2 11.90708 60 24.4 60.0 117.9345 39435 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 9.6 17.6 32.3 9.5 22.4 42.0 14.1 25.0 42.6 29.3 Excluded by Method 2.83 1 0.0003003 0.0001541 False High Sequest HT (A2) Unambiguous ADESDPVESK [K].aDEsDPVESk.[K] N-Term(TMT6plex); S4(Phospho); K10(TMT6plex) 1 P38174 P38174 0 2 0.3815 0 1 1 807.88727 1614.76726 1614.76642 0.52 0.00042 663767.875 CID (Collision Induced Dissociation) MS2 0.0 40 217.8 60.0 55.376 16008 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 57.8 189.9 330.9 75.2 183.1 416.4 72.1 195.0 437.4 220.1 Excluded by Method 4.22 1 0.0003003 0.0001474 False High Sequest HT (A2) Unambiguous EEDEEPESPPEK [K].eEDEEPEsPPEk.[K] N-Term(TMT6plex); S8(Phospho); K12(TMT6plex) 2 Q9H1E3 Q9H1E3; Q9H1E3-2 0 2 0.5473 0 1 1 976.94233 1952.87738 1952.87782 -0.22 -0.00022 15673362.0 CID (Collision Induced Dissociation) MS2 0.0 40 237.2 23.591 55.548 16071 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 244.2 252.8 192.0 252.8 217.1 220.1 266.7 247.0 252.7 227.0 Excluded by Method 4.33 1 0.0003003 1.833e-06 False High Sequest HT (A2) Unambiguous DVLDIEQFSTVK [K].dVLDIEQFsTVk.[G] N-Term(TMT6plex); S9(Phospho); K12(TMT6plex) 4 P34947 P34947; P43250; P43250-2; P43250-3 0 3 0.0213 0 1 1 644.67602 1932.0135 1932.01313 0.19 0.00012 1013551.5625 CID (Collision Induced Dissociation) MS2 17.3175 50 203.3 60.0 122.0331 40948 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 200.7 199.1 169.7 224.7 196.4 191.3 229.1 218.4 203.3 200.2 Excluded by Method 3.75 1 0.0003003 8.632e-05 False High Sequest HT (A2) Unambiguous SPQPPVEEEDEHFDDTVVCLDTYNCDLHFK [K].sPQPPVEEEDEHFDDTVVcLDTYNcDLHFk.[I] N-Term(TMT6plex); S1(Phospho); C19(Carbamidomethyl); C25(Carbamidomethyl); K30(TMT6plex) 2 Q00839 Q00839-2; Q00839 0 4 0.4679 0 1 1 1044.2174 4173.84779 4173.84991 -0.51 -0.00053 924367.75 CID (Collision Induced Dissociation) MS2 9.104642 60 11.9 60.0 121.9421 40914 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 13.0 20.5 7.1 10.0 12.4 10.5 12.2 9.2 11.0 12.7 Excluded by Method 7.63 1 0.0003003 1.529e-06 False High Sequest HT (A2) Unambiguous HDVPQDSNDNNNEELEAQGQQAQEK [K].hDVPQDSNDNNNEELEAQGQQAQEk.[N] N-Term(TMT6plex); K25(TMT6plex) 1 P35732 P35732 0 4 0.5685 0 1 1 824.64281 3295.5494 3295.54634 0.93 0.00077 251428.765625 CID (Collision Induced Dissociation) MS2 9.916575 40 15.7 60.0 52.7191 15016 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 11.4 25.7 4.0 12.0 29.9 6.6 13.5 35.3 18.9 Excluded by Method 3.94 1 0.0003003 5.069e-06 False High Sequest HT (A2) Unambiguous VNGDASPAAAESGAK [K].vNGDAsPAAAESGAk.[E] N-Term(TMT6plex); S6(Phospho); K15(TMT6plex) 1 P29966 P29966 0 2 0.2635 0 1 1 941.96928 1882.93129 1882.93119 0.05 5e-05 1328598.125 CID (Collision Induced Dissociation) MS2 0.0 60 109.9 60.0 56.4397 16402 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 115.8 111.3 84.1 115.9 99.2 111.5 122.8 120.3 109.2 109.1 Excluded by Method 5.39 1 0.0003003 3.796e-06 False High Sequest HT (A2) Unambiguous RGSIGENQIK [K].rGsIGENQIk.[D] N-Term(TMT6plex); S3(Phospho); K10(TMT6plex) 2 Q05682-4 Q05682-4; Q05682-5 1 3 0.4309 0 1 1 547.30265 1639.89338 1639.89329 0.06 3e-05 3798532.25 CID (Collision Induced Dissociation) MS2 5.009923 60 122.2 35.344 52.7054 15010 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 127.7 120.3 93.0 137.1 119.5 111.5 141.2 136.0 110.1 125.1 Excluded by Method 4.34 1 0.0003003 0.0002238 False High Sequest HT (A2) Unambiguous ASGRGAESPFEEK [K].aSGRGAEsPFEEk.[S] N-Term(TMT6plex); S8(Phospho); K13(TMT6plex) 1 P46821 P46821 1 3 0.3871 0 1 1 634.98371 1902.93656 1902.93628 0.15 0.0001 28951104.0 CID (Collision Induced Dissociation) MS2 3.925281 30 138.7 7.0 56.2481 16329 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 136.0 142.9 112.8 145.0 128.0 144.6 155.4 139.5 136.0 147.0 Excluded by Method 3.72 1 0.0003003 0.0005211 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVssDEEF.[-] N-Term(TMT6plex); S8(Phospho); S9(Phospho) 1 P16521 P16521 0 2 0.1435 0 1 1 925.3551 1849.70293 1849.70195 0.53 0.00049 4514945.0 CID (Collision Induced Dissociation) MS2 7.864133 40 194.0 51.727 122.4976 41112 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 46.8 156.3 326.2 56.4 143.3 380.6 64.8 175.9 399.5 190.6 Excluded by Method 4.18 1 0.0003003 0.0001253 False High Sequest HT (A2) Unambiguous LSNSSLSNLFK [K].lSNSsLSNLFk.[K] N-Term(TMT6plex); S5(Phospho); K11(TMT6plex) 1 P13259 P13259 0 3 0.0084 0 1 1 583.31799 1747.93941 1747.93957 -0.09 -5e-05 669622.0625 CID (Collision Induced Dissociation) MS2 0.0 40 120.4 60.0 123.6998 41553 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 28.9 95.9 187.5 38.0 101.1 238.2 47.4 119.0 237.3 110.7 Excluded by Method 3.56 1 0.0003003 0.0002447 False High Sequest HT (A2) Unambiguous FVQGLLQNAAANAEAK [K].fVQGLLQNAAANAEAk.[G] N-Term(TMT6plex); K16(TMT6plex) 2 P46990 P46990; P05740 0 3 0.498 0 1 1 701.73724 2103.19716 2103.19626 0.43 0.0003 688710.1875 CID (Collision Induced Dissociation) MS2 8.134326 70 103.4 60.0 123.3662 41429 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 30.2 85.7 155.7 30.3 90.6 215.9 43.5 90.5 201.5 90.1 4.92 1 0.0003003 2.292e-08 False High Sequest HT (A2) Unambiguous ETTESPAETTTGTSTEAPSAAN [K].eTtESPAETTTGTSTEAPSAAN.[-] N-Term(TMT6plex); T3(Phospho) 1 P38205 P38205 0 3 0.0267 0 1 1 821.36083 2462.06793 2462.06568 0.91 0.00075 328039.0625 CID (Collision Induced Dissociation) MS2 23.7824 60 51.4 60.0 53.5206 15319 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 16.7 44.4 75.2 16.5 43.0 104.7 19.1 50.3 97.4 46.4 Excluded by Method 4.49 1 0.0003003 6.109e-05 False High Sequest HT (A2) Unambiguous EPEGPPPSPGK [K].ePEGPPPsPGk.[G] N-Term(TMT6plex); S8(Phospho); K11(TMT6plex) 1 P58012 P58012 0 3 0.618 0 1 1 543.94828 1629.83028 1629.82896 0.81 0.00044 1161101.875 CID (Collision Induced Dissociation) MS2 0.0 70 187.6 60.0 53.8149 15425 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 200.3 187.8 146.3 202.7 192.2 187.4 219.2 190.3 182.0 167.9 5.21 1 0.0003003 1.155e-05 False High Sequest HT (A2) Unambiguous LFQLGPPSPVK [K].lFQLGPPsPVk.[M] N-Term(TMT6plex); S8(Phospho); K11(TMT6plex) 3 O95997 O95997; Q9NZH5; Q9NZH5-2 0 3 0.4263 0 1 1 574.33307 1720.98467 1720.98032 2.53 0.00145 514268.8125 CID (Collision Induced Dissociation) MS2 24.21635 40 388.4 60.0 122.5595 41133 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 418.7 389.1 342.4 408.7 358.9 353.6 448.5 416.7 393.2 354.3 Excluded by Method 4.95 1 0.0003003 7.796e-05 False High Sequest HT (A2) Unambiguous EEPAAAGSGAASPSAAEK [K].eEPAAAGSGAAsPSAAEk.[G] N-Term(TMT6plex); S12(Phospho); K18(TMT6plex) 1 P29966 P29966 0 3 0.1656 0 1 1 713.68418 2139.038 2139.03711 0.41 0.00029 12503782.0 CID (Collision Induced Dissociation) MS2 3.264354 100 210.1 23.131 55.1953 15939 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 216.9 214.3 163.3 209.3 188.6 223.0 224.7 216.0 215.6 229.1 8.03 1 0.0003003 4.92e-10 False High Sequest HT (A2) Unambiguous AEASGEAAEEAEDEE [K].aEAsGEAAEEAEDEE.[-] N-Term(TMT6plex); S4(Phospho) 1 P05755 P05755 0 2 0.8072 0 1 1 923.35962 1845.71197 1845.71126 0.38 0.00035 2103278.0 CID (Collision Induced Dissociation) MS2 5.808887 90 62.1 60.0 54.6289 15722 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 14.9 47.4 96.0 16.6 48.9 121.1 16.8 63.8 127.1 68.4 4.15 1 0.0003003 3.366e-05 False High Sequest HT (A2) Unambiguous NEATPEAEQVK [K].nEAtPEAEQVk.[K] N-Term(TMT6plex); T4(Phospho); K11(TMT6plex) 1 Q08245 Q08245 0 3 0.4788 0 1 1 585.29754 1753.87807 1753.87737 0.4 0.00023 35403988.0 CID (Collision Induced Dissociation) MS2 5.067799 50 150.5 6.089 52.9733 15111 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 28.5 122.8 234.1 41.1 130.4 304.8 53.0 131.6 313.9 144.6 Excluded by Method 4.01 1 0.0003003 0.0001847 False High Sequest HT (A2) Unambiguous VPTVGSYGVAGATLPETIPTSK [K].vPtVGSYGVAGATLPETIPTSk.[N] N-Term(TMT6plex); T3(Phospho); K22(TMT6plex) 1 P38250 P38250 0 4 0.002 0 1 1 671.61434 2683.43554 2683.43597 -0.16 -0.00011 794023.25 CID (Collision Induced Dissociation) MS2 14.12442 30 71.6 60.0 122.7264 41193 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 20.9 66.4 114.6 22.5 57.4 142.6 26.6 67.5 128.9 68.2 Excluded by Method 5.01 1 0.0003003 1.848e-05 False High Sequest HT (A2) Unambiguous GDDDEESDEEAVK [K].gDDDEEsDEEAVk.[K] N-Term(TMT6plex); S7(Phospho); K13(TMT6plex) 1 O43395 O43395 0 3 0.4251 0 1 1 659.2858 1975.84286 1975.84216 0.35 0.00023 202059.296875 CID (Collision Induced Dissociation) MS2 38.57933 50 230.6 60.0 52.6595 14994 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 239.6 228.0 184.5 261.1 226.1 216.9 276.4 251.3 215.7 206.1 Excluded by Method 3.67 1 0.0003003 5.105e-05 False High Sequest HT (A2) Unambiguous GVNLPGTDVDLPALSEK [K].gVNLPGTDVDLPALSEk.[D] N-Term(TMT6plex); K17(TMT6plex) 1 P00549 P00549 0 3 0.5616 0 1 1 728.4147 2183.22954 2183.23237 -1.3 -0.00094 642593.1875 CID (Collision Induced Dissociation) MS2 10.60997 60 67.5 60.0 120.2033 40279 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 31.0 60.2 100.5 32.4 47.8 120.5 37.7 56.7 114.4 73.5 Excluded by Method 4.95 1 0.0003003 0.0003503 False High Sequest HT (A2) Unambiguous SFEERVETTVTSLK [K].sFEERVETTVTSLk.[T] N-Term(TMT6plex); S1(Phospho); K14(TMT6plex) 2 Q16890 Q16890; Q16890-5 1 3 0.3218 0 1 1 722.04837 2164.13056 2164.13029 0.13 9e-05 1245814.125 CID (Collision Induced Dissociation) MS2 3.007349 60 41.3 60.0 120.1913 40275 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 40.9 37.6 34.9 52.2 34.4 42.3 40.2 49.3 43.8 37.4 Excluded by Method 4.35 1 0.0003003 0.0001433 False High Sequest HT (A2) Unambiguous GVEEVEGEYELTEAVEDGPRPFK [K].gVEEVEGEYELTEAVEDGPRPFk.[V] N-Term(TMT6plex); K23(TMT6plex) 1 P26321 P26321 0 4 0.5364 0 1 1 760.14172 3037.54503 3037.54063 1.45 0.0011 341281.6875 CID (Collision Induced Dissociation) MS2 26.38276 70 42.6 60.0 120.137 40254 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 22.6 36.2 56.1 28.7 39.5 62.5 28.2 46.2 59.9 46.1 4.4 1 0.0003003 0.0001349 False High Sequest HT (A2) Unambiguous NEPEDEEEEEEEEDEDEEEEDEDEE [K].nEPEDEEEEEEEEDEDEEEEDEDEE.[-] N-Term(TMT6plex) 1 P26583 P26583 0 3 0.9384 0 1 1 1119.73026 3357.17624 3357.17785 -0.48 -0.00054 1262121.5 CID (Collision Induced Dissociation) MS2 0.0 80 12.3 60.0 60.7116 17992 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 7.7 13.1 9.6 11.0 14.0 14.0 15.4 10.9 11.0 16.6 6.98 1 0.0003003 9.842e-09 False High Sequest HT (A2) Unambiguous SPSFASEWDEIEK [K].sPsFASEWDEIEk.[I] N-Term(TMT6plex); S3(Phospho); K13(TMT6plex) 1 Q92625 Q92625 0 2 0.1296 0 1 1 1031.99256 2062.97785 2062.97747 0.18 0.00019 1470813.25 CID (Collision Induced Dissociation) MS2 0.0 20 52.3 60.0 120.0117 40207 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 53.8 46.6 46.1 52.6 39.3 55.0 59.8 61.5 54.7 53.8 Excluded by Method 5.17 1 0.0003003 1.68e-05 False High Sequest HT (A2) Unambiguous GTSQVGELSQSSTK [K].gTSQVGELSQsSTk.[L] N-Term(TMT6plex); S11(Phospho); K14(TMT6plex) 1 P32604 P32604 0 3 0.0448 0 1 1 649.66667 1946.98547 1946.98362 0.95 0.00061 2985869.25 CID (Collision Induced Dissociation) MS2 0.0 90 106.1 45.604 61.1987 18179 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 33.0 89.9 161.5 34.1 90.7 202.4 38.8 90.6 202.2 117.4 4.02 1 0.0003003 1.436e-05 False High Sequest HT (A2) Unambiguous SPPAEEAASPEK [K].sPPAEEAAsPEk.[E] N-Term(TMT6plex); S9(Phospho); K12(TMT6plex) 2 P12036 P12036; P12036-2 0 3 0.4499 0 1 1 584.29388 1750.86708 1750.86647 0.35 0.00021 1014021.6875 CID (Collision Induced Dissociation) MS2 0.0 40 101.3 60.0 52.3177 14868 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 91.4 97.4 83.7 111.8 100.0 104.0 110.3 106.5 103.3 104.1 Excluded by Method 5.69 1 0.0003003 9.607e-06 False High Sequest HT (A2) Unambiguous SDQQGEALDSSQK [K].sDQQGEALDSSQk.[K] N-Term(TMT6plex); S1(Phospho); K13(TMT6plex) 3 Q32MZ4 Q32MZ4-2; Q32MZ4; Q32MZ4-3 0 3 0.2484 0 1 1 644.3106 1930.91725 1930.91594 0.68 0.00044 425719.375 CID (Collision Induced Dissociation) MS2 0.0 40 21.9 60.0 52.2354 14837 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 24.0 21.9 15.7 16.3 23.8 21.6 22.8 24.9 26.6 21.7 Excluded by Method 3.06 1 0.0003003 0.0002773 False High Sequest HT (A2) Unambiguous RSRLTPVSPESSSTEEK [K].rSRLTPVsPESSSTEEk.[S] N-Term(TMT6plex); S8(Phospho); K17(TMT6plex) 2 Q13501 Q13501-2; Q13501 2 3 0.0495 0 1 1 810.08795 2428.2493 2428.2485 0.33 0.00027 563128.5625 CID (Collision Induced Dissociation) MS2 0.0 40 54.6 60.0 52.2077 14826 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 61.1 55.3 45.6 61.9 53.0 52.6 56.6 59.4 52.3 48.1 Excluded by Method 5.66 1 0.0003003 0.0003989 False High Sequest HT (A2) Unambiguous ELDQDMVTEDEDDPGSHK [K].eLDQDmVtEDEDDPGSHk.[R] N-Term(TMT6plex); M6(Oxidation); T8(Phospho); K18(TMT6plex) 2 Q9NRL2 Q9NRL2; Q9NRL2-2 0 3 0.4294 0 1 1 872.04712 2614.12679 2614.12679 0.0 0.0 977119.25 CID (Collision Induced Dissociation) MS2 0.0 40 60.0 60.0 62.0889 18510 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 67.9 58.8 59.1 47.6 51.9 63.3 71.1 66.0 53.0 61.7 Excluded by Method 5.38 1 0.0003003 4.392e-07 False High Sequest HT (A2) Unambiguous AEGEPQEESPLK [K].aEGEPQEEsPLk.[S] N-Term(TMT6plex); S9(Phospho); K12(TMT6plex) 4 Q9NYF8 Q9NYF8-3; Q9NYF8-4; Q9NYF8; Q9NYF8-2 0 3 0.4599 0 1 1 617.97554 1851.91208 1851.91415 -1.11 -0.00069 23817448.0 CID (Collision Induced Dissociation) MS2 7.651742 80 63.5 7.603 61.3785 18245 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 64.7 63.2 42.0 74.5 59.7 63.6 76.8 59.0 69.4 62.3 5.24 1 0.0003003 2.359e-05 False High Sequest HT (A2) Unambiguous LYERRGGGSGGGEESEGEEVDED [K].lYERRGGGsGGGEEsEGEEVDED.[-] N-Term(TMT6plex); S9(Phospho); S15(Phospho) 1 Q96QR8 Q96QR8 2 3 0.1933 0 1 1 934.70446 2802.09882 2802.0978 0.36 0.00034 654134.1875 CID (Collision Induced Dissociation) MS2 40.32162 50 57.8 60.0 60.1146 17768 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 59.7 51.7 50.0 60.4 57.0 64.8 64.1 57.2 57.8 55.6 Excluded by Method 4.81 1 0.0003003 7.664e-05 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVSsDEEF.[-] N-Term(TMT6plex); S9(Phospho) 1 P16521 P16521 0 2 0.0299 0 1 1 885.37126 1769.73524 1769.73562 -0.22 -0.00019 945842.4375 CID (Collision Induced Dissociation) MS2 0.0 50 64.0 60.0 120.9895 40566 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 16.8 44.8 93.1 26.8 50.0 130.1 27.9 57.4 127.5 65.5 Excluded by Method 3.68 1 0.0003003 0.0001107 False High Sequest HT (A2) Unambiguous RAAVGQESPGGLEAGNAK [K].rAAVGQEsPGGLEAGNAk.[A] N-Term(TMT6plex); S8(Phospho); K18(TMT6plex) 1 Q29RF7 Q29RF7 1 4 0.4103 0 1 1 563.29665 2250.16476 2250.16438 0.17 9e-05 814775.875 CID (Collision Induced Dissociation) MS2 10.88881 20 43.1 60.0 58.4113 17125 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 33.4 37.6 40.9 35.6 45.8 51.5 38.2 43.8 50.0 54.0 Excluded by Method 3.68 1 0.0003003 0.0005176 False High Sequest HT (A2) Unambiguous KAEGEPQEESPLK [K].kAEGEPQEEsPLk.[S] N-Term(TMT6plex); K1(TMT6plex); S10(Phospho); K13(TMT6plex) 4 Q9NYF8 Q9NYF8-3; Q9NYF8-4; Q9NYF8; Q9NYF8-2 1 4 0.3755 0 1 1 553.04849 2209.17213 2209.17204 0.04 2e-05 1190571.875 CID (Collision Induced Dissociation) MS2 21.41599 20 189.1 60.0 58.6314 17206 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 198.8 192.6 140.7 202.0 176.6 185.1 226.9 196.5 201.1 170.8 Excluded by Method 2.45 1 0.0003003 0.0005211 False High Sequest HT (A2) Unambiguous SPQPPVEEEDEHFDDTVVCLDTYNCDLHFK [K].sPQPPVEEEDEHFDDTVVcLDTYNcDLHFk.[I] N-Term(TMT6plex); S1(Phospho); C19(Carbamidomethyl); C25(Carbamidomethyl); K30(TMT6plex) 2 Q00839 Q00839-2; Q00839 0 4 0.4107 0 1 1 1044.21765 4173.84877 4173.84991 -0.28 -0.00029 1678977.5 CID (Collision Induced Dissociation) MS2 2.115093 40 26.9 58.213 120.8076 40497 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 26.7 25.0 23.1 28.4 26.7 25.8 29.5 29.4 24.6 29.6 Excluded by Method 8.23 1 0.0003003 3.165e-06 False High Sequest HT (A2) Unambiguous NGVAAEVSPAK [K].nGVAAEVsPAk.[E] N-Term(TMT6plex); S8(Phospho); K11(TMT6plex) 1 Q9BW71 Q9BW71 0 3 0.467 0 1 1 527.61974 1580.84468 1580.84494 -0.17 -9e-05 3161778.0 CID (Collision Induced Dissociation) MS2 3.729182 90 116.6 60.0 59.8766 17680 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 102.3 111.9 90.7 125.9 112.5 117.1 125.4 117.6 142.1 120.9 5.31 1 0.0003003 4.382e-06 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVssDEEF.[-] N-Term(TMT6plex); S8(Phospho); S9(Phospho) 1 P16521 P16521 0 2 0.1255 0 1 1 925.3548 1849.70232 1849.70195 0.2 0.00018 4380895.5 CID (Collision Induced Dissociation) MS2 9.224517 40 154.3 51.343 120.2863 40309 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 39.2 137.5 255.7 42.2 109.7 290.7 43.4 140.7 326.1 158.0 Excluded by Method 4.7 1 0.0003003 1.498e-05 False High Sequest HT (A2) Unambiguous NSEDDELAK [K].nsEDDELAk.[E] N-Term(TMT6plex); S2(Phospho); K9(TMT6plex) 3 Q96T23 Q96T23; Q96T23-3; Q96T23-2 0 2 0.599 0 1 1 779.87418 1558.74108 1558.7402 0.56 0.00044 1003889.5625 CID (Collision Induced Dissociation) MS2 7.030983 50 44.7 60.0 59.6118 17582 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 40.4 49.2 39.3 43.5 45.3 43.4 48.8 42.8 49.2 44.6 Excluded by Method 3.84 1 0.0003003 0.000275 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVssDEEF.[-] N-Term(TMT6plex); S8(Phospho); S9(Phospho) 1 P16521 P16521 0 2 0.1302 0 1 1 925.35486 1849.70244 1849.70195 0.26 0.00024 3574845.5 CID (Collision Induced Dissociation) MS2 6.607533 50 176.4 60.0 121.4109 40721 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 41.2 151.5 292.5 49.3 132.7 347.4 63.0 170.3 341.8 174.2 Excluded by Method 4.53 1 0.0003003 2.424e-05 False High Sequest HT (A2) Unambiguous KNEPEDEEEEEEEEDEDEEEEDEDEE [K].kNEPEDEEEEEEEEDEDEEEEDEDEE.[-] N-Term(TMT6plex); K1(TMT6plex) 1 P26583 P26583 1 3 0.9051 0 1 1 1238.8151 3714.43075 3714.43575 -1.35 -0.00167 1943419.625 CID (Collision Induced Dissociation) MS2 0.0 40 49.6 60.0 58.8692 17298 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 47.3 51.6 39.1 62.1 49.9 44.6 53.6 52.9 49.0 45.7 Excluded by Method 6.43 1 0.0003003 1.789e-09 False High Sequest HT (A2) Unambiguous RTCPSLSPTSPLNNK [K].rTcPSLSPTsPLNNk.[G] N-Term(TMT6plex); C3(Carbamidomethyl); S10(Phospho); K15(TMT6plex) 2 O75925 O75925; O75925-2 1 3 0.1111 0 1 1 737.3849 2210.14016 2210.14047 -0.14 -0.00011 1425395.625 CID (Collision Induced Dissociation) MS2 0.0 50 113.6 60.0 70.6049 21712 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 109.7 108.5 92.8 118.0 119.1 116.1 123.5 120.6 118.6 108.8 Excluded by Method 4.5 1 0.0003003 0.000125 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVssDEEF.[-] N-Term(TMT6plex); S8(Phospho); S9(Phospho) 1 P16521 P16521 0 2 0.1471 0 1 1 925.3548 1849.70232 1849.70195 0.2 0.00018 1625264.0 CID (Collision Induced Dissociation) MS2 0.0 40 91.2 60.0 115.8546 38656 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 22.8 76.7 162.2 28.1 72.8 172.8 32.0 80.4 176.0 88.6 Excluded by Method 4.08 1 0.0003003 3.551e-05 False High Sequest HT (A2) Unambiguous DVTPPPETEVVLIK [K].dVtPPPETEVVLIk.[N] N-Term(TMT6plex); T3(Phospho); K14(TMT6plex) 3 P27816 P27816; P27816-6; P27816-2 0 3 0.1637 0 1 1 692.38623 2075.14415 2075.14415 0.0 0.0 1684081.125 CID (Collision Induced Dissociation) MS2 4.322001 40 112.6 60.0 116.1492 38766 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 123.8 115.1 90.5 118.9 105.9 99.7 128.6 116.9 118.4 107.7 Excluded by Method 3.36 1 0.0003003 0.0001184 False High Sequest HT (A2) Unambiguous EETDTDLEVVLEK [K].eEtDTDLEVVLEk.[K] N-Term(TMT6plex); T3(Phospho); K13(TMT6plex) 1 Q1ED39 Q1ED39 0 3 0.0087 0 1 1 686.68153 2058.03003 2058.02957 0.22 0.00015 1538599.0 CID (Collision Induced Dissociation) MS2 7.695975 70 134.7 60.0 116.1014 38748 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 148.2 144.4 109.6 128.7 122.8 127.8 152.1 149.6 129.1 134.6 3.44 1 0.0003003 0.0001742 False High Sequest HT (A2) Unambiguous EDSAPVAAGESESSL [K].eDSAPVAAGESESsL.[-] N-Term(TMT6plex); S14(Phospho) 1 P25567 P25567 0 2 0.0349 0 1 1 879.38781 1757.76833 1757.76799 0.2 0.00017 4403526.0 CID (Collision Induced Dissociation) MS2 0.0 80 122.1 60.0 71.9174 22208 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 30.1 98.2 199.1 30.5 99.8 250.3 41.2 111.8 244.7 115.3 5.16 1 0.0003003 7.837e-09 False High Sequest HT (A2) Unambiguous FTPPAESGSPSK [K].fTPPAESGsPSk.[E] N-Term(TMT6plex); S9(Phospho); K12(TMT6plex) 1 Q70E73 Q70E73 0 3 0.1649 0 1 1 581.63012 1742.87582 1742.87664 -0.47 -0.00027 6221286.5 CID (Collision Induced Dissociation) MS2 5.746521 50 66.9 33.241 73.7518 22901 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 70.4 59.4 53.3 70.8 63.9 68.1 66.6 71.5 78.9 66.4 Excluded by Method 5.58 1 0.0003003 1.946e-06 False High Sequest HT (A2) Unambiguous RLGSLVDEFK [K].rLGsLVDEFk.[E] N-Term(TMT6plex); S4(Phospho); K10(TMT6plex) 2 P12956 P12956; P12956-2 1 3 0.3538 0 1 1 567.98268 1701.93348 1701.93409 -0.36 -0.0002 340572.65625 CID (Collision Induced Dissociation) MS2 31.47982 80 121.7 60.0 115.5008 38524 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 128.0 120.4 97.8 126.4 109.1 125.1 141.5 132.1 125.0 111.5 3.9 1 0.0003003 0.0005056 False High Sequest HT (A2) Unambiguous AGFNYESPSK [K].aGFNYEsPSk.[G] N-Term(TMT6plex); S7(Phospho); K10(TMT6plex) 3 Q9UHF7 Q9UHF7-3; Q9UHF7-2; Q9UHF7 0 3 0.2796 0 1 1 546.60416 1637.79793 1637.79766 0.16 9e-05 1233787.75 CID (Collision Induced Dissociation) MS2 28.28612 60 177.5 60.0 74.0867 23026 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 184.9 187.6 146.5 174.5 164.7 164.5 197.1 196.1 175.6 183.1 Excluded by Method 3.97 1 0.0003003 0.0003751 False High Sequest HT (A2) Unambiguous EATETSSAVQTK [K].eATETsSAVQTk.[E] N-Term(TMT6plex); S6(Phospho); K12(TMT6plex) 1 Q05050 Q05050 0 2 0.0085 0 1 1 895.4531 1789.89892 1789.8985 0.23 0.00021 920800.75 CID (Collision Induced Dissociation) MS2 46.22032 30 112.8 60.0 49.6923 13896 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 49.1 100.0 158.6 32.9 96.7 201.2 51.5 112.0 204.8 121.1 Excluded by Method 4.73 1 0.0003003 7.246e-05 False High Sequest HT (A2) Unambiguous AAAYDISEDEED [K].aAAYDIsEDEED.[-] N-Term(TMT6plex); S7(Phospho) 1 P49585 P49585 0 2 0.2407 0 1 1 818.82714 1636.647 1636.64647 0.32 0.00026 9573394.0 CID (Collision Induced Dissociation) MS2 0.0 80 154.3 31.147 74.365 23129 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 156.9 163.9 139.3 159.0 131.4 157.5 174.5 144.3 155.9 160.7 3.78 1 0.0003003 7.001e-06 False High Sequest HT (A2) Unambiguous GTDTQTPAVLSPSK [K].gTDTQTPAVLsPSk.[T] N-Term(TMT6plex); S11(Phospho); K14(TMT6plex) 3 P46087 P46087-2; P46087; P46087-4 0 3 0.113 0 1 1 647.34313 1940.01483 1940.01419 0.33 0.00021 29951978.0 CID (Collision Induced Dissociation) MS2 5.894299 100 164.3 6.101 73.2651 22713 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 148.7 166.7 137.0 157.7 168.5 160.6 189.9 177.2 173.8 162.8 5.75 1 0.0003003 1.048e-06 False High Sequest HT (A2) Unambiguous RLLGDSDSEEEQK [K].rLLGDsDsEEEQk.[E] N-Term(TMT6plex); S6(Phospho); S8(Phospho); K13(TMT6plex) 1 Q9BW71 Q9BW71 1 3 0.4704 0 1 1 708.65996 2123.96532 2123.96633 -0.48 -0.00034 2020913.625 CID (Collision Induced Dissociation) MS2 0.0 60 136.4 60.0 72.6905 22496 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 137.0 118.9 114.4 152.2 125.1 135.3 157.1 141.8 145.7 136.5 Excluded by Method 4.23 1 0.0003003 0.0001649 False High Sequest HT (A2) Unambiguous SNFSNSADDIK [K].sNFSNSADDIk.[S] N-Term(TMT6plex); S1(Phospho); K11(TMT6plex) 1 P45973 P45973 0 3 0.0508 0 1 1 579.28149 1735.82993 1735.83042 -0.28 -0.00016 4437276.0 CID (Collision Induced Dissociation) MS2 1.587162 80 68.6 34.083 73.8572 22941 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 65.9 67.9 47.4 73.4 74.1 73.1 69.9 74.2 71.8 68.5 3.54 1 0.0003003 7.12e-05 False High Sequest HT (A2) Unambiguous LPWEVSDGEVSSGK [K].lPWEVsDGEVSSGk.[T] N-Term(TMT6plex); S6(Phospho); K14(TMT6plex) 1 P48415 P48415 0 3 0.5513 0 1 1 676.67464 2028.00937 2028.00911 0.13 9e-05 2653468.75 CID (Collision Induced Dissociation) MS2 6.993897 70 161.0 60.0 115.3971 38487 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 42.6 139.2 243.8 59.7 116.5 311.6 59.8 149.6 305.1 182.0 4.97 1 0.0003003 1.659e-06 False High Sequest HT (A2) Unambiguous VQGEAVSNIQENTQTPTVQEESEEEEVDETGVEVK [K].vQGEAVSNIQENTQTPTVQEEsEEEEVDETGVEVk.[D] N-Term(TMT6plex); S22(Phospho); K35(TMT6plex) 3 Q13765 Q13765; E9PAV3; E9PAV3-2 0 4 0.0602 0 1 1 1100.52008 4399.05849 4399.06016 -0.38 -0.00042 475847.25 CID (Collision Induced Dissociation) MS2 0.0 50 16.7 60.0 115.6957 38595 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 19.8 15.0 10.3 20.1 14.0 16.9 20.2 18.5 15.1 17.5 Excluded by Method 5.15 1 0.0003003 1.012e-06 False High Sequest HT (A2) Unambiguous NPHDIIEGINAGEIEIPEN [K].nPHDIIEGINAGEIEIPEN.[-] N-Term(TMT6plex) 1 P0CX53 P0CX53 0 3 0.4181 0 1 1 768.39598 2303.17339 2303.17167 0.74 0.00057 837567.1875 CID (Collision Induced Dissociation) MS2 0.0 30 171.0 60.0 115.8953 38671 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 34.7 144.5 270.7 55.6 130.3 336.1 63.6 146.3 355.1 173.0 Excluded by Method 2.99 1 0.0003003 1.204e-05 False High Sequest HT (A2) Unambiguous EAPAEGEAAEPGSPTAAEGEAASAASSTSSPK [K].eAPAEGEAAEPGSPTAAEGEAASAASStSSPk.[A] N-Term(TMT6plex); T28(Phospho); K32(TMT6plex) 1 P29966 P29966 0 4 0.0023 0 1 1 864.15394 3453.59391 3453.59467 -0.22 -0.00019 706700.6875 CID (Collision Induced Dissociation) MS2 0.0 40 45.7 60.0 72.9695 22600 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 49.3 41.9 37.2 49.2 45.3 37.2 54.8 48.8 45.0 48.6 Excluded by Method 4.34 1 0.0003003 0.0001037 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVSsDEEF.[-] N-Term(TMT6plex); S9(Phospho) 1 P16521 P16521 0 2 0.0143 0 1 1 885.37175 1769.73622 1769.73562 0.34 0.0003 2276553.75 CID (Collision Induced Dissociation) MS2 37.1839 50 115.2 26.373 116.508 38898 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 27.7 92.2 187.7 25.8 90.6 223.4 33.6 105.9 244.6 120.8 Excluded by Method 4.19 1 0.0003003 6.652e-05 False High Sequest HT (A2) Unambiguous DYNASASTISPPSSMEEDK [K].dYNASASTIsPPSSmEEDk.[F] N-Term(TMT6plex); S10(Phospho); M15(Oxidation); K19(TMT6plex) 1 P46821 P46821 0 3 0.1714 0 1 1 861.72321 2583.15508 2583.15737 -0.88 -0.00076 1810901.125 CID (Collision Induced Dissociation) MS2 19.21793 60 54.4 60.0 72.4394 22405 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 53.1 59.4 41.6 54.7 51.8 55.5 57.2 55.7 58.4 56.2 Excluded by Method 4.26 1 0.0003003 4.767e-06 False High Sequest HT (A2) Unambiguous LQQTVPADASPDSK [K].lQQTVPADAsPDSk.[C] N-Term(TMT6plex); S10(Phospho); K14(TMT6plex) 2 Q9NS56 Q9NS56-2; Q9NS56 0 3 0.1522 0 1 1 665.6785 1995.02094 1995.02001 0.47 0.00031 2998798.25 CID (Collision Induced Dissociation) MS2 35.62114 30 70.6 25.006 71.8431 22181 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 73.2 61.7 61.9 63.0 63.0 70.3 76.7 81.2 75.0 79.9 Excluded by Method 3.68 1 0.0003003 0.0001131 False High Sequest HT (A2) Unambiguous SQVDGAGDVSNEEIAADDTK [K].sQVDGAGDVsNEEIAADDTk.[K] N-Term(TMT6plex); S10(Phospho); K20(TMT6plex) 1 P53438 P53438 0 3 0.1425 0 1 1 853.73439 2559.18862 2559.18636 0.88 0.00075 1765324.75 CID (Collision Induced Dissociation) MS2 0.0 50 45.8 60.0 73.7669 22907 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 13.6 39.1 60.7 10.4 38.7 87.7 16.5 45.2 98.0 48.2 Excluded by Method 4.63 1 0.0003003 2.188e-06 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVsSDEEF.[-] N-Term(TMT6plex); S8(Phospho) 1 P16521 P16521 0 2 0.015 0 1 1 885.3726 1769.73793 1769.73562 1.3 0.00115 3960834.25 CID (Collision Induced Dissociation) MS2 3.427887 50 106.1 20.736 115.6913 38594 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 25.2 88.6 158.0 31.2 90.5 210.8 33.6 96.8 209.9 115.9 Excluded by Method 3.99 1 0.0003003 7.677e-05 False High Sequest HT (A2) Unambiguous HEDLFAASGNDEK [K].hEDLFAAsGNDEk.[L] N-Term(TMT6plex); S8(Phospho); K13(TMT6plex) 1 P48415 P48415 0 3 0.6089 0 1 1 657.64689 1970.92612 1970.92611 0.0 0.0 2350578.75 CID (Collision Induced Dissociation) MS2 9.943049 60 78.0 58.186 70.8624 21810 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 25.1 68.6 114.0 29.0 57.1 145.7 35.7 70.0 164.4 70.6 Excluded by Method 6.06 1 0.0003003 2.606e-05 False High Sequest HT (A2) Unambiguous SSTSFANIQENSN [K].sSTsFANIQENSN.[-] N-Term(TMT6plex); S4(Phospho) 1 Q86WC4 Q86WC4 0 2 0.0266 0 1 1 854.37526 1707.74324 1707.74244 0.47 0.0004 6901916.0 CID (Collision Induced Dissociation) MS2 0.0 80 163.1 41.141 72.4944 22424 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 174.7 176.5 134.9 159.1 140.2 171.6 177.4 173.0 165.0 159.0 4.14 1 0.0003003 6.631e-07 False High Sequest HT (A2) Unambiguous QFANSNNNNNDSGNNNQGDYVTK [K].qFANSNNNNNDSGNNNQGDYVTk.[A] N-Term(TMT6plex); K23(TMT6plex) 1 P18899 P18899 0 3 0.6953 0 1 1 996.46815 2987.38989 2987.38799 0.64 0.00063 1079455.25 CID (Collision Induced Dissociation) MS2 0.0 90 71.9 60.0 60.8863 18028 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 15.7 63.3 127.5 22.6 54.7 137.9 26.1 66.4 126.4 78.6 8.14 1 0.0003003003 1.768412e-13 False High Sequest HT (A2) Unambiguous GDNITLLQSVSN [K].gDNITLLQSVSN.[-] N-Term(TMT6plex) 1 P62304 P62304 0 2 0.4037 0 1 1 745.40618 1489.80508 1489.80595 -0.58 -0.00043 704043.1875 CID (Collision Induced Dissociation) MS2 19.74546 60 328.8 60.0 100.1052 32721 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 330.0 319.6 289.8 308.9 288.2 330.5 366.9 369.9 360.6 323.7 Excluded by Method 2.7 1 0.0004462 0.0006657 False High Sequest HT (A2) Unambiguous QLVAGNSPK [K].qLVAGNsPk.[K] N-Term(TMT6plex); S7(Phospho); K9(TMT6plex) 2 Q96EU6 Q96EU6-2; Q96EU6 0 2 0.3643 0 1 1 726.40518 1451.80308 1451.80235 0.5 0.00036 1135705.375 CID (Collision Induced Dissociation) MS2 12.0358 50 74.5 60.0 60.075 17754 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 63.0 74.3 72.8 66.3 75.4 77.0 70.4 71.7 99.8 74.7 Excluded by Method 2.91 1 0.0004462 0.00213 False High Sequest HT (A2) Unambiguous LTNRLDDLLERSQELTISK [K].lTNRLDDLLERSQELtISk.[E] N-Term(TMT6plex); T16(Phospho); K19(TMT6plex) 1 P38853 P38853 2 4 0.0045 0 1 1 696.38375 2782.51316 2782.5116 0.56 0.00039 1035801.125 CID (Collision Induced Dissociation) MS2 4.416849 30 59.8 60.0 123.676 41463 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 16.0 43.2 87.2 17.2 46.1 125.8 23.0 57.9 124.2 56.9 Excluded by Method 4.4 1 0.0004462 0.0008629 False High Sequest HT (A2) Unambiguous NLSNENCSFK [K].nLsNENcSFk.[K] N-Term(TMT6plex); S3(Phospho); C7(Carbamidomethyl); K10(TMT6plex) 1 P11972 P11972 0 2 0.394 0 1 1 875.91528 1750.82329 1750.82356 -0.15 -0.00013 1365316.5 CID (Collision Induced Dissociation) MS2 0.0 20 71.6 60.0 70.5295 21602 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 12.9 63.5 108.4 24.1 64.0 132.5 30.9 63.7 138.9 77.1 Excluded by Method 4.01 1 0.0004462 0.000645 False High Sequest HT (A2) Unambiguous DDDEEPSDSEDK [K].dDDEEPsDSEDk.[E] N-Term(TMT6plex); S7(Phospho); K12(TMT6plex) 1 P09232 P09232 0 2 0.1699 0 1 1 959.8959 1918.78453 1918.78431 0.11 0.00011 307836.28125 CID (Collision Induced Dissociation) MS2 0.0 60 24.5 60.0 43.6623 11642 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 9.7 17.9 34.7 7.5 20.4 43.3 7.4 22.0 58.8 23.1 Excluded by Method 3.12 1 0.0004462 0.0006996 False High Sequest HT (A2) Unambiguous LSGFSFKK [K].lSGFsFkk.[N] N-Term(TMT6plex); S5(Phospho); K7(TMT6plex); K8(TMT6plex) 1 P29966 P29966 1 2 0.2246 0 1 1 840.98816 1680.96904 1680.96931 -0.16 -0.00013 9520458.0 CID (Collision Induced Dissociation) MS2 0.0 10 89.6 60.0 101.7222 33325 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 97.3 89.6 69.0 106.0 83.7 87.4 107.3 88.5 81.8 85.7 Excluded by Method 3.34 1 0.0004462 0.001012 False High Sequest HT (A2) Unambiguous KKGSGEDDEEETETTPTSTVPVATIAQETLK [K].kkGSGEDDEEETETTPTsTVPVATIAQETLk.[V] N-Term(TMT6plex); K1(TMT6plex); K2(TMT6plex); S18(Phospho); K31(TMT6plex) 1 P12962 P12962 2 4 0.0028 0 1 1 1072.80825 4288.21119 4288.21425 -0.71 -0.00077 601878.875 CID (Collision Induced Dissociation) MS2 0.0 80 21.9 60.0 106.6334 35191 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 3.8 16.8 29.4 7.7 20.8 40.5 7.8 20.2 45.8 26.0 3.63 1 0.0004462 0.0009195 False High Sequest HT (A2) Unambiguous SELCSSPLGK [K].sELcSsPLGk.[R] N-Term(TMT6plex); C4(Carbamidomethyl); S6(Phospho); K10(TMT6plex) 1 Q9Y2F5 Q9Y2F5 0 3 0.0588 0 1 1 539.27678 1615.81577 1615.81668 -0.56 -0.0003 1594797.0 CID (Collision Induced Dissociation) MS2 21.995 80 153.0 60.0 76.0892 23777 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 167.8 152.7 113.0 160.6 140.9 154.5 169.4 173.7 148.5 148.6 3.23 1 0.0004462 0.001167 False High Sequest HT (A2) Unambiguous ITIADCGQLE [K].iTIADcGQLE.[-] N-Term(TMT6plex); C6(Carbamidomethyl) 2 P62937 P62937; P62937-2 0 2 0.614 0 1 1 674.85287 1348.69845 1348.69798 0.35 0.00024 9308829.0 CID (Collision Induced Dissociation) MS2 6.422571 70 98.2 15.898 93.7939 30428 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 105.2 97.2 89.3 90.1 86.7 103.0 107.8 104.0 88.7 110.0 2.85 1 0.0004462 0.0008052 False High Sequest HT (A2) Unambiguous SLPENSTASAASAFDK [K].sLPENSTASAASAFDk.[A] N-Term(TMT6plex); S1(Phospho); K16(TMT6plex) 1 P41832 P41832 0 3 0.0224 0 1 1 712.02049 2134.04692 2134.04695 -0.02 -1e-05 678601.4375 CID (Collision Induced Dissociation) MS2 24.1237 40 110.8 60.0 93.4397 30296 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 26.8 90.5 173.0 40.9 94.1 213.5 35.0 95.0 218.1 121.2 Excluded by Method 4.01 1 0.0004462 0.001066 False High Sequest HT (A2) Unambiguous LYERRGGGSGGGEESEGEEVDED [K].lYERRGGGsGGGEEsEGEEVDED.[-] N-Term(TMT6plex); S9(Phospho); S15(Phospho) 1 Q96QR8 Q96QR8 2 3 0.1582 0 1 1 934.70595 2802.10329 2802.0978 1.96 0.00183 581997.4375 CID (Collision Induced Dissociation) MS2 0.0 40 91.5 60.0 60.2591 17764 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 91.4 95.2 88.4 92.4 93.4 95.0 99.2 88.1 87.3 84.8 Excluded by Method 4.55 1 0.0004462 0.0007216 False High Sequest HT (A2) Unambiguous ISLSPSTESLADSK [K].iSLSPSTEsLADSk.[S] N-Term(TMT6plex); S9(Phospho); K14(TMT6plex) 1 P31374 P31374 0 3 0.085 0 1 1 658.34707 1973.02667 1973.02442 1.14 0.00075 304266.40625 CID (Collision Induced Dissociation) MS2 50.70529 20 191.4 60.0 102.06 33447 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 138.9 168.3 231.4 138.9 166.7 266.5 143.1 196.6 281.6 181.6 Excluded by Method 3.06 1 0.0004462 0.00057 False High Sequest HT (A2) Unambiguous GSNYHLSDNDASDVE [K].gSNYHLSDNDAsDVE.[-] N-Term(TMT6plex); S12(Phospho) 1 Q96QE2 Q96QE2 0 2 0.5768 0 1 1 966.39711 1931.78695 1931.78576 0.61 0.00059 174838.234375 CID (Collision Induced Dissociation) MS2 0.0 60 50.7 60.0 60.2788 17772 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 58.3 49.5 44.6 39.9 52.6 52.4 53.9 58.9 33.8 62.9 Excluded by Method 2.41 1 0.0004462 0.001202 False High Sequest HT (A2) Unambiguous TSVESEIGGNQVGLK [K].tSVEsEIGGNQVGLk.[I] N-Term(TMT6plex); T1(Phospho); S5(Phospho); K15(TMT6plex) 1 P34250 P34250 0 3 0.4295 0 1 1 712.68548 2136.04187 2136.0391 1.3 0.00092 1100408.625 CID (Collision Induced Dissociation) MS2 13.7277 60 58.8 60.0 100.6523 32925 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 23.9 51.5 74.4 29.5 52.1 99.2 29.5 59.8 116.0 52.5 Excluded by Method 3.05 1 0.0004462 0.001641 False High Sequest HT (A2) Unambiguous STDNLAFLK [K].sTDNLAFLk.[K] N-Term(TMT6plex); S1(Phospho); K9(TMT6plex) 1 Q96N96-6 Q96N96-6 0 3 0.0048 0 1 1 516.2808 1546.82785 1546.82823 -0.24 -0.00013 334562.5625 CID (Collision Induced Dissociation) MS2 45.58217 40 11.6 60.0 107.5442 35537 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 14.0 11.2 9.7 11.7 7.5 13.1 11.2 13.4 9.7 14.8 Excluded by Method 2.07 1 0.0004462 0.001744 False High Sequest HT (A2) Unambiguous LVQIAEEESDK [K].lVQIAEEEsDk.[E] N-Term(TMT6plex); S9(Phospho); K11(TMT6plex) 1 Q06810 Q06810 0 3 0.4006 0 1 1 600.31333 1798.92544 1798.92398 0.81 0.00049 1214792.625 CID (Collision Induced Dissociation) MS2 3.670775 70 39.7 60.0 93.2845 30237 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 19.0 41.4 44.0 24.5 33.9 56.7 29.2 40.4 67.3 40.8 3.22 1 0.0004462 0.0005671 False High Sequest HT (A2) Unambiguous EEVQELEELNK [K].eEVQELEELNk.[T] N-Term(TMT6plex); K11(TMT6plex) 1 P15108 P15108 0 3 0.0 0 1 1 606.66818 1817.99 1817.98968 0.18 0.00011 1125233.375 CID (Collision Induced Dissociation) MS2 4.561522 90 92.9 60.0 93.3988 30280 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 32.5 73.3 128.4 33.9 81.2 167.0 46.2 91.4 169.7 105.8 2.98 1 0.0004462 0.0006452 False High Sequest HT (A2) Unambiguous IGPLGLSPK [K].iGPLGLsPk.[K] N-Term(TMT6plex); S7(Phospho); K9(TMT6plex) 2 P0CX53 P30050; P0CX53 0 2 0.4067 0 1 1 710.42202 1419.83677 1419.83767 -0.64 -0.00045 2014827.125 CID (Collision Induced Dissociation) MS2 20.64292 30 157.1 60.0 107.3318 35453 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 150.2 160.7 134.5 196.9 139.2 156.0 177.2 144.0 163.0 148.9 Excluded by Method 3.0 1 0.0004462 0.001496 False High Sequest HT (A2) Unambiguous LIREASPATEFANEFTGENSTAK [K].lIREASPAtEFANEFTGENSTAk.[S] N-Term(TMT6plex); T9(Phospho); K23(TMT6plex) 1 P13259 P13259 1 4 0.0703 0 1 1 756.13034 3021.49954 3021.49707 0.82 0.00062 922678.3125 CID (Collision Induced Dissociation) MS2 7.871816 30 20.4 60.0 115.5139 38459 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 9.1 13.9 32.7 9.7 18.3 33.7 9.9 19.7 40.2 17.1 Excluded by Method 2.56 1 0.0004462 0.001388 False High Sequest HT (A2) Unambiguous ATVTPSPVK [K].aTVTPsPVk.[G] N-Term(TMT6plex); S6(Phospho); K9(TMT6plex) 2 Q9H1E3 Q9H1E3; Q9H1E3-2 0 2 0.1424 0 1 1 719.40964 1437.81201 1437.81185 0.11 8e-05 34881532.0 CID (Collision Induced Dissociation) MS2 1.229765 50 285.9 7.3 62.817 18784 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 309.3 280.1 236.4 300.5 252.9 287.4 314.5 308.0 274.9 294.6 Excluded by Method 2.95 1 0.0004462 0.001047 False High Sequest HT (A2) Unambiguous IGEGTYGVVYK [K].iGEGtyGVVYk.[AG] N-Term(TMT6plex); T5(Phospho); Y6(Phospho); K11(TMT6plex) 5 P24941 P24941; P06493; Q00526; P24941-2; P06493-2 0 3 0.5105 0 1 1 601.96314 1803.87487 1803.87354 0.74 0.00044 1107524.0 CID (Collision Induced Dissociation) MS2 5.983631 60 205.0 60.0 96.2129 31251 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 214.6 193.5 169.5 224.1 195.8 178.0 250.8 218.3 220.1 185.2 Excluded by Method 4.76 1 0.0004462 0.001709 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVssDEEF.[-] N-Term(TMT6plex); S8(Phospho); S9(Phospho) 1 P16521 P16521 0 2 0.1681 0 1 1 925.35533 1849.70338 1849.70195 0.77 0.00071 1146917.75 CID (Collision Induced Dissociation) MS2 37.71822 40 60.9 55.859 112.4276 37359 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 17.3 50.3 96.7 17.4 57.3 120.8 26.5 51.4 112.7 59.0 Excluded by Method 3.45 1 0.0004462 0.001347 False High Sequest HT (A2) Unambiguous KEESEESDDDMGFGLFD [K].kEESEEsDDDmGFGLFD.[-] N-Term(TMT6plex); K1(TMT6plex); S7(Phospho); M11(Oxidation) 3 P05387 P05386; P05386-2; P05387 1 2 0.0308 0 1 1 1252.52615 2504.04503 2504.04642 -0.55 -0.00069 1917200.5 CID (Collision Induced Dissociation) MS2 0.0 40 91.7 60.0 112.4152 37353 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 87.6 90.0 67.9 95.9 91.9 84.3 111.5 106.2 95.9 85.3 Excluded by Method 3.57 1 0.0004462 0.001459 False High Sequest HT (A2) Unambiguous IELSDDSDVEVHPNVDKK [K].iELsDDsDVEVHPNVDkk.[S] N-Term(TMT6plex); S4(Phospho); S7(Phospho); K17(TMT6plex); K18(TMT6plex) 1 P06101 P06101 1 4 0.4103 0 1 1 722.35867 2886.41287 2886.41421 -0.47 -0.00034 699781.375 CID (Collision Induced Dissociation) MS2 4.867539 30 37.8 60.0 90.7218 29273 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 13.8 34.1 59.6 19.8 33.3 68.3 16.5 36.2 64.2 32.6 Excluded by Method 4.29 1 0.0004462 0.001473 False High Sequest HT (A2) Unambiguous LFPADTSPSTATK [K].lFPADtSPSTATk.[I] N-Term(TMT6plex); T6(Phospho); K13(TMT6plex) 2 Q6NZY4 Q6NZY4-2; Q6NZY4 0 2 0.0401 0 1 1 937.48933 1873.97138 1873.97127 0.06 6e-05 324100.6875 CID (Collision Induced Dissociation) MS2 28.2191 50 95.8 60.0 96.6086 31400 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 97.4 93.0 89.2 91.7 96.1 98.8 102.6 102.6 104.1 82.9 Excluded by Method 3.49 1 0.0004462 0.001093 False High Sequest HT (A2) Unambiguous SFTNIEQDYK [K].sFTNIEQDYk.[Q] N-Term(TMT6plex); S1(Phospho); K10(TMT6plex) 1 Q07528 Q07528 0 3 0.0028 0 1 1 594.96181 1782.87088 1782.87155 -0.38 -0.00022 466497.90625 CID (Collision Induced Dissociation) MS2 0.0 100 97.3 60.0 96.6692 31423 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 27.1 86.6 152.9 30.1 80.1 181.5 35.7 97.3 183.5 98.3 3.56 1 0.0004462 0.0007399 False High Sequest HT (A2) Unambiguous ASPSCSSPTRDSSGVPVSK [K].aSPScsSPTRDSSGVPVSk.[E] N-Term(TMT6plex); C5(Carbamidomethyl); S6(Phospho); K19(TMT6plex) 1 Q8N5I9 Q8N5I9 1 3 0.0207 0 1 1 815.40215 2444.19189 2444.18928 1.07 0.00087 817611.375 CID (Collision Induced Dissociation) MS2 7.117868 20 107.7 60.0 56.7193 16453 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 103.3 119.3 89.3 98.2 94.2 106.7 126.6 119.4 107.1 112.6 Excluded by Method 3.86 1 0.0004462 0.001699 False High Sequest HT (A2) Unambiguous AEETPASSNESTPSASSSSSANK [K].aEETPASsNESTPSASSSSSANk.[L] N-Term(TMT6plex); S8(Phospho); K23(TMT6plex) 1 P32583 P32583 0 3 0.0171 0 1 1 922.09003 2764.25554 2764.25623 -0.25 -0.00023 1770805.875 CID (Collision Induced Dissociation) MS2 0.0 60 9.2 60.0 48.1057 13309 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 2.3 8.2 14.7 1.4 6.9 17.4 10.7 18.6 11.9 Excluded by Method 3.5 1 0.0004462 0.001057 False High Sequest HT (A2) Unambiguous ATISDEEIERQLK [K].aTIsDEEIERQLk.[A] N-Term(TMT6plex); S4(Phospho); K13(TMT6plex) 2 Q9UQN3 Q9UQN3; Q9UQN3-2 1 4 0.0256 0 1 1 518.27735 2070.08756 2070.08842 -0.42 -0.00022 768107.0 CID (Collision Induced Dissociation) MS2 30.10543 0 60.5 60.0 90.3706 29140 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 64.7 66.7 40.8 66.3 50.9 55.4 71.8 64.1 59.4 65.2 Excluded by Method 4.68 1 0.0004462 0.0009962 False High Sequest HT (A2) Unambiguous ENGSGDDLFAGLK [K].eNGsGDDLFAGLk.[K] N-Term(TMT6plex); S4(Phospho); K13(TMT6plex) 1 P09064 P09064 0 3 0.4 0 1 1 620.97671 1860.91557 1860.91448 0.58 0.00036 532629.0625 CID (Collision Induced Dissociation) MS2 7.367698 50 288.0 60.0 113.5742 37785 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 80.7 240.8 476.3 79.4 239.5 538.3 115.0 266.2 559.6 284.4 Excluded by Method 3.3 1 0.0004462 0.000769 False High Sequest HT (A2) Unambiguous VVADDLVLVTPK [K].vVADDLVLVtPk.[K] N-Term(TMT6plex); T10(Phospho); K12(TMT6plex) 1 P38934 P38934 0 3 0.2857 0 1 1 603.0185 1807.04095 1807.03822 1.51 0.00091 583885.5 CID (Collision Induced Dissociation) MS2 7.773614 70 42.2 60.0 117.8459 39316 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 15.5 34.2 57.9 20.3 34.9 76.9 26.4 36.5 75.4 44.1 3.64 1 0.0004462 0.0005761 False High Sequest HT (A2) Unambiguous KVSGTLDTPEK [K].kVSGTLDtPEk.[T] N-Term(TMT6plex); K1(TMT6plex); T8(Phospho); K11(TMT6plex) 1 Q9BYG3 Q9BYG3 1 3 0.3549 0 1 1 648.03368 1942.08649 1942.08652 -0.02 -1e-05 1528241.625 CID (Collision Induced Dissociation) MS2 19.48201 10 89.5 56.198 63.2906 18964 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 93.8 80.2 72.8 101.9 90.1 88.1 103.6 85.8 89.7 89.2 Excluded by Method 3.86 1 0.0004462 0.001564 False High Sequest HT (A2) Unambiguous ELSPAALEK [K].eLsPAALEk.[R] N-Term(TMT6plex); S3(Phospho); K9(TMT6plex) 1 O75683 O75683 0 3 0.4184 0 1 1 499.27728 1495.81727 1495.81733 -0.04 -2e-05 1146357.375 CID (Collision Induced Dissociation) MS2 7.285257 60 293.9 60.0 90.0757 29031 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 317.2 305.9 256.1 307.0 276.1 289.2 320.6 314.9 274.8 277.4 Excluded by Method 3.92 1 0.0004462 0.001099 False High Sequest HT (A2) Unambiguous TFNSPNLK [K].tFNsPNLk.[D] N-Term(TMT6plex); S4(Phospho); K8(TMT6plex) 5 Q9Y6N7 Q9Y6N7; Q9Y6N7-4; Q9Y6N7-5; Q9Y6N7-3; Q9Y6N7-2 0 2 0.3529 0 1 1 729.89156 1458.77585 1458.7758 0.03 2e-05 4829625.5 CID (Collision Induced Dissociation) MS2 0.0 10 115.0 60.0 76.8848 24076 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 109.4 117.0 87.6 112.9 111.5 117.5 130.1 130.8 116.4 116.4 Excluded by Method 3.57 1 0.0004462 0.00111 False High Sequest HT (A2) Unambiguous LAVGDNNSK [K].lAVGDNNSk.[W] N-Term(TMT6plex); K9(TMT6plex) 1 P46940 P46940 0 2 0.4215 0 1 1 688.40152 1375.79577 1375.79455 0.89 0.00061 1010967.0 CID (Collision Induced Dissociation) MS2 12.64099 50 38.7 60.0 56.0825 16218 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 29.2 29.6 39.5 35.7 38.1 47.8 37.6 44.9 37.0 47.6 Excluded by Method 2.42 1 0.0004462 0.001002 False High Sequest HT (A2) Unambiguous ELEAERSLMSSPEDLTK [K].eLEAERSLmssPEDLTk.[D] N-Term(TMT6plex); M9(Oxidation); S10(Phospho); S11(Phospho); K17(TMT6plex) 1 P46821 P46821 1 3 0.2823 0 1 1 857.0688 2569.19184 2569.19099 0.33 0.00028 4659488.0 CID (Collision Induced Dissociation) MS2 1.670192 40 183.6 36.05 90.9787 29367 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 180.4 184.1 144.9 190.8 181.1 189.1 203.9 187.2 196.4 178.2 Excluded by Method 4.18 1 0.0004462 0.001456 False High Sequest HT (A2) Unambiguous TQSPEFEAQSSK [K].tQsPEFEAQSSk.[F] N-Term(TMT6plex); S3(Phospho); K12(TMT6plex) 1 Q9H5H4 Q9H5H4 0 3 0.0604 0 1 1 626.30854 1876.91108 1876.9094 0.9 0.00056 2881906.5 CID (Collision Induced Dissociation) MS2 0.0 60 43.9 56.97 61.0494 18122 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 45.7 48.7 31.9 47.8 37.1 40.6 48.1 37.0 48.6 53.8 Excluded by Method 3.31 1 0.0004462 0.0006979 False High Sequest HT (A2) Unambiguous DITDPLSLNTCTDEGHVVLASPLK [K].dITDPLSLNTcTDEGHVVLAsPLk.[T] N-Term(TMT6plex); C11(Carbamidomethyl); S21(Phospho); K24(TMT6plex) 1 Q7L2J0 Q7L2J0 0 4 0.3238 0 1 1 784.15306 3133.59041 3133.58925 0.37 0.00029 1989265.0 CID (Collision Induced Dissociation) MS2 6.950157 30 27.2 37.243 123.3593 41345 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 19.2 24.7 28.3 22.8 25.0 35.6 25.9 27.9 35.1 27.3 Excluded by Method 4.91 1 0.0004462 0.0009334 False High Sequest HT (A2) Unambiguous VVRNNSITSATSK [K].vVRNNsITSATSk.[T] N-Term(TMT6plex); S6(Phospho); K13(TMT6plex) 1 Q05050 Q05050 1 3 0.301 0 1 1 639.01886 1915.04203 1915.04141 0.32 0.00021 689112.3125 CID (Collision Induced Dissociation) MS2 2.806833 50 59.9 60.0 57.5581 16762 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 16.3 45.8 82.5 16.7 49.0 127.0 18.8 56.5 112.1 74.2 Excluded by Method 3.09 1 0.0004462 0.0007523 False High Sequest HT (A2) Unambiguous IDGATQSSPAEPK [K].iDGATQSsPAEPk.[S] N-Term(TMT6plex); S8(Phospho); K13(TMT6plex) 3 Q32MZ4 Q32MZ4-2; Q32MZ4; Q32MZ4-3 0 3 0.0742 0 1 1 613.64816 1838.92992 1838.93013 -0.12 -7e-05 10823778.0 CID (Collision Induced Dissociation) MS2 4.618112 30 138.5 13.48 60.6107 17953 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 131.5 140.9 99.9 144.5 135.7 151.2 151.7 133.0 141.2 155.1 Excluded by Method 2.56 1 0.0004462 0.002042 False High Sequest HT (A2) Unambiguous IEVLDSPASK [K].iEVLDsPASk.[K] N-Term(TMT6plex); S6(Phospho); K10(TMT6plex) 1 Q8N5I9 Q8N5I9 0 2 0.1818 0 1 1 798.9358 1596.86433 1596.86501 -0.43 -0.00034 922930.0625 CID (Collision Induced Dissociation) MS2 0.0 40 252.4 60.0 91.7568 29666 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 266.5 273.3 232.1 257.8 244.5 233.0 275.9 251.3 231.9 257.7 Excluded by Method 4.07 1 0.0004462 0.0009195 False High Sequest HT (A2) Unambiguous DDQLEYLEEK [K].dDQLEYLEEk.[R] N-Term(TMT6plex); K10(TMT6plex) 2 P15108 P02829; P15108 0 3 0.3131 0 1 1 580.64178 1739.9108 1739.91037 0.25 0.00014 1786794.5 CID (Collision Induced Dissociation) MS2 0.0 60 79.4 60.0 91.7747 29673 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 22.3 65.7 105.9 31.5 66.8 146.8 31.0 78.0 158.7 87.0 Excluded by Method 2.97 1 0.0004462 0.001058 False High Sequest HT (A2) Unambiguous EAAGGNDSSGATSPINPAVALE [K].eAAGGNDsSGATSPINPAVALE.[-] N-Term(TMT6plex); S8(Phospho) 3 P32004-2 P32004; P32004-3; P32004-2 0 3 0.0741 0 1 1 779.69787 2337.07905 2337.08088 -0.78 -0.00061 374056.5 CID (Collision Induced Dissociation) MS2 0.0 60 56.8 60.0 109.6857 36335 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 31.5 47.3 56.4 36.6 63.2 79.6 44.0 63.5 86.1 59.4 Excluded by Method 3.51 1 0.0004462 0.00177 False High Sequest HT (A2) Unambiguous TNLSGRQSPSFK [K].tNLSGRQsPSFk.[L] N-Term(TMT6plex); S8(Phospho); K12(TMT6plex) 3 Q6IN85 Q6IN85-2; Q6IN85; Q6IN85-4 1 3 0.1899 0 1 1 620.66365 1859.9764 1859.97808 -0.9 -0.00056 4166751.75 CID (Collision Induced Dissociation) MS2 14.69097 30 287.7 21.988 61.186 18174 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 276.5 309.9 236.0 309.2 278.7 295.0 308.1 304.5 267.1 292.1 Excluded by Method 2.58 1 0.0004462 0.001874 False High Sequest HT (A2) Unambiguous EVTPESGPPK [K].eVtPESGPPk.[V] N-Term(TMT6plex); T3(Phospho); K10(TMT6plex) 4 P46087 P46087-3; P46087-2; P46087; P46087-4 0 2 0.2819 0 1 1 789.91464 1578.82201 1578.81806 2.5 0.00198 252690.546875 CID (Collision Induced Dissociation) MS2 21.41455 50 281.5 60.0 56.9888 16552 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 298.0 290.0 228.4 287.9 266.1 280.6 316.5 289.1 270.2 287.7 Excluded by Method 2.98 1 0.0004462 0.001669 False High Sequest HT (A2) Unambiguous KEESEESDDDMGFGLFD [K].kEESEEsDDDmGFGLFD.[-] N-Term(TMT6plex); K1(TMT6plex); S7(Phospho); M11(Oxidation) 3 P05387 P05386; P05386-2; P05387 1 2 0.0413 0 1 1 1252.52762 2504.04796 2504.04642 0.62 0.00077 466852.0 CID (Collision Induced Dissociation) MS2 0.0 50 103.0 60.0 111.3164 36945 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 113.8 85.9 86.1 103.4 111.1 97.5 112.9 114.0 101.1 103.7 Excluded by Method 3.39 1 0.0004462 0.000865 False High Sequest HT (A2) Unambiguous KWDGSEEDEDNSK [K].kWDGsEEDEDNSk.[K] N-Term(TMT6plex); K1(TMT6plex); S5(Phospho); K13(TMT6plex) 1 O60841 O60841 1 3 0.4282 0 1 1 769.36436 2306.07853 2306.07926 -0.32 -0.00024 1311218.75 CID (Collision Induced Dissociation) MS2 39.90565 40 252.6 60.0 61.6482 18345 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 300.1 250.5 187.2 313.1 243.6 220.4 310.2 257.6 224.0 219.0 Excluded by Method 3.41 1 0.0004462 0.001374 False High Sequest HT (A2) Unambiguous YNLDASEEEDSNK [K].yNLDAsEEEDSNk.[K] N-Term(TMT6plex); S6(Phospho); K13(TMT6plex) 2 O95218 O95218-2; O95218 0 2 0.352 0 1 1 1026.46433 2051.92137 2051.92108 0.14 0.00015 2693132.0 CID (Collision Induced Dissociation) MS2 0.0 20 51.7 60.0 71.0676 21806 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 48.7 59.0 39.6 59.9 50.5 51.3 59.5 51.5 50.5 46.8 Excluded by Method 3.21 1 0.0004462 0.0009215 False High Sequest HT (A2) Unambiguous SLHPWYGITPTSSPK [K].sLHPWYGITPTSsPk.[T] N-Term(TMT6plex); S13(Phospho); K15(TMT6plex) 1 Q8N3F8 Q8N3F8 0 3 0.0346 0 1 1 737.05457 2209.14916 2209.14588 1.49 0.0011 807642.25 CID (Collision Induced Dissociation) MS2 20.60785 60 54.9 60.0 97.9531 31914 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 54.5 54.1 47.2 49.4 61.0 65.1 51.8 51.5 60.8 53.4 Excluded by Method 4.05 1 0.0004462 0.000727 False High Sequest HT (A2) Unambiguous IFQNPTDEESTTSLNEK [K].iFQNPtDEESTTsLNEk.[Q] N-Term(TMT6plex); T6(Phospho); S13(Phospho); K17(TMT6plex) 1 P40563 P40563 0 3 0.0267 0 1 1 857.72818 2571.16999 2571.16688 1.21 0.00104 514089.34375 CID (Collision Induced Dissociation) MS2 51.35828 40 38.2 60.0 103.1623 33857 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 12.7 27.1 64.6 15.3 31.9 63.8 21.2 42.1 73.8 29.9 Excluded by Method 3.37 1 0.0004462 0.0006845 False High Sequest HT (A2) Unambiguous LEGADDRLEADDSDDLENIDSGDLALYK [K].lEGADDRLEADDsDDLENIDSGDLALYk.[D] N-Term(TMT6plex); S13(Phospho); K28(TMT6plex) 1 Q12513 Q12513 1 3 0.377 0 1 1 1202.56044 3605.66677 3605.6784 -3.22 -0.00388 306106.5 CID (Collision Induced Dissociation) MS2 61.93682 80 9.7 60.0 124.1889 41654 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 9.0 13.5 3.0 7.7 20.1 2.2 10.9 20.8 9.9 4.88 1 0.0004462 0.0008898 False High Sequest HT (A2) Unambiguous EFSIPRAHAWPSPYK [K].eFsIPRAHAWPSPYk.[D] N-Term(TMT6plex); S3(Phospho); K15(TMT6plex) 1 Q8NFJ5 Q8NFJ5 1 4 0.2282 0 1 1 581.80488 2324.19771 2324.19931 -0.69 -0.0004 624409.5625 CID (Collision Induced Dissociation) MS2 73.42595 30 99.6 60.0 99.9907 32703 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 102.6 98.3 71.5 103.7 95.0 91.9 110.2 109.3 112.6 100.4 Excluded by Method 2.98 1 0.0004462 0.001463 False High Sequest HT (A2) Unambiguous RLGSLVDEFK [K].rLGsLVDEFk.[E] N-Term(TMT6plex); S4(Phospho); K10(TMT6plex) 2 P12956 P12956; P12956-2 1 3 0.4821 0 1 1 567.98286 1701.93402 1701.93409 -0.04 -2e-05 1071467.75 CID (Collision Induced Dissociation) MS2 4.882226 80 104.5 60.0 116.3151 38746 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 115.8 91.0 76.8 123.0 101.1 100.7 114.9 111.3 108.6 101.6 3.9 1 0.0004462 0.001467 False High Sequest HT (A2) Unambiguous NPHDIIEGINAGEIEIPEN [K].nPHDIIEGINAGEIEIPEN.[-] N-Term(TMT6plex) 1 P0CX53 P0CX53 0 3 0.2349 0 1 1 768.3954 2303.17164 2303.17167 -0.01 -1e-05 1369700.375 CID (Collision Induced Dissociation) MS2 0.0 20 151.9 60.0 116.3132 38745 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 38.5 134.5 245.6 52.3 108.2 303.9 52.5 139.9 300.0 143.7 Excluded by Method 2.81 1 0.0004462 0.001402 False High Sequest HT (A2) Unambiguous KKGSGEDDEEETETTPTSTVPVATIAQETLK [K].kkGSGEDDEEETETtPTSTVPVATIAQETLk.[V] N-Term(TMT6plex); K1(TMT6plex); K2(TMT6plex); T15(Phospho); K31(TMT6plex) 1 P12962 P12962 2 4 0.0231 0 1 1 1072.81019 4288.21893 4288.21425 1.09 0.00117 517840.28125 CID (Collision Induced Dissociation) MS2 0.0 40 30.7 60.0 107.5526 35504 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 7.9 26.4 35.4 15.1 25.7 49.3 13.2 32.2 63.3 38.0 Excluded by Method 3.9 1 0.0004462 0.0008471 False High Sequest HT (A2) Unambiguous EEDEEPESPPEK [K].eEDEEPEsPPEk.[K] N-Term(TMT6plex); S8(Phospho); K12(TMT6plex) 2 Q9H1E3 Q9H1E3; Q9H1E3-2 0 3 0.529 0 1 1 651.6309 1952.87816 1952.87782 0.17 0.00011 8656510.0 CID (Collision Induced Dissociation) MS2 11.40077 50 198.6 20.169 54.451 15657 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 206.8 213.1 149.4 222.9 184.6 175.3 229.6 213.0 201.1 190.2 Excluded by Method 2.93 1 0.0004462 0.0006246 False High Sequest HT (A2) Unambiguous LFESDEEETNGENIQINK [K].lFEsDEEETNGENIQINk.[G] N-Term(TMT6plex); S4(Phospho); K18(TMT6plex) 1 Q04500 Q04500 0 3 0.1309 0 1 1 883.08965 2647.25439 2647.25404 0.13 0.00011 1388889.75 CID (Collision Induced Dissociation) MS2 5.933277 40 45.0 33.393 107.6649 35545 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 12.5 34.7 67.2 13.3 38.7 83.5 20.3 46.2 85.5 47.7 Excluded by Method 2.75 1 0.0004462 0.001231 False High Sequest HT (A2) Unambiguous DNELLETTASFADK [K].dNELLETTAsFADk.[I] N-Term(TMT6plex); S10(Phospho); K14(TMT6plex) 1 Q92331 Q92331 0 3 0.2212 0 1 1 698.01344 2092.02578 2092.02515 0.3 0.00021 1040665.6875 CID (Collision Induced Dissociation) MS2 55.01751 20 60.8 60.0 110.5575 36630 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 15.3 53.1 80.9 19.7 56.6 104.6 22.2 68.6 118.8 68.6 Excluded by Method 3.12 1 0.0004462 0.00206 False High Sequest HT (A2) Unambiguous GYGFVDFDSPSAAQK [K].gYGFVDFDsPSAAQk.[A] N-Term(TMT6plex); S9(Phospho); K15(TMT6plex) 1 Q15434 Q15434 0 3 0.144 0 1 1 709.67895 2127.0223 2127.02001 1.08 0.00076 1254724.125 CID (Collision Induced Dissociation) MS2 0.0 60 130.8 60.0 110.6167 36652 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 129.8 131.1 104.2 139.9 126.5 136.8 147.1 142.3 130.6 119.6 Excluded by Method 3.75 1 0.0004462 0.0005758 False High Sequest HT (A2) Unambiguous GRAEGEWEDQEALDYFSDK [K].gRAEGEWEDQEALDYFsDk.[E] N-Term(TMT6plex); S17(Phospho); K19(TMT6plex) 3 Q9NYF8 Q9NYF8-3; Q9NYF8; Q9NYF8-2 1 3 0.2037 0 1 1 928.42514 2783.26085 2783.26019 0.24 0.00022 426280.46875 CID (Collision Induced Dissociation) MS2 0.0 40 50.7 60.0 106.5516 35131 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 41.4 55.8 41.7 57.2 42.6 49.6 57.1 57.7 54.3 49.6 Excluded by Method 3.83 1 0.0004462 0.0009584 False High Sequest HT (A2) Unambiguous LEVTEIVKPSPK [K].lEVTEIVkPsPk.[R] N-Term(TMT6plex); K8(TMT6plex); S10(Phospho); K12(TMT6plex) 2 Q7Z6E9 Q7Z6E9-2; Q7Z6E9 0 3 0.4795 0 1 1 703.08475 2107.2397 2107.23827 0.68 0.00048 2881367.25 CID (Collision Induced Dissociation) MS2 7.439555 30 158.2 60.0 101.0884 33114 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 164.3 163.1 117.6 182.7 149.4 150.9 181.1 160.1 156.3 156.9 Excluded by Method 3.65 1 0.0004462 0.0007764 False High Sequest HT (A2) Unambiguous GPQPPTVSPIRSSRPPPAK [K].gPQPPTVsPIRSSRPPPAk.[R] N-Term(TMT6plex); S8(Phospho); K19(TMT6plex) 3 P46087 P46087-2; P46087; P46087-4 1 4 0.0805 0 1 1 627.60262 2507.38866 2507.38996 -0.52 -0.00033 316746.90625 CID (Collision Induced Dissociation) MS2 13.31856 20 31.3 60.0 63.9206 19142 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 30.7 34.3 31.7 34.9 27.6 33.4 35.0 27.3 30.6 27.4 Excluded by Method 3.48 1 0.0004462 0.0009612 False High Sequest HT (A2) Unambiguous VQGEAVSNIQENTQTPTVQEESEEEEVDETGVEVK [K].vQGEAVSNIQENTQTPTVQEEsEEEEVDETGVEVk.[D] N-Term(TMT6plex); S22(Phospho); K35(TMT6plex) 3 Q13765 Q13765; E9PAV3; E9PAV3-2 0 4 0.0378 0 1 1 1100.51998 4399.05808 4399.06016 -0.47 -0.00052 412770.71875 CID (Collision Induced Dissociation) MS2 0.0 50 16.6 60.0 124.6873 41834 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 16.4 20.9 17.3 14.6 12.7 17.1 20.6 15.3 17.5 13.3 Excluded by Method 3.44 1 0.0004462 0.001329 False High Sequest HT (A2) Unambiguous QGSPDQVSPVSEMTSTSLYQDK [K].qGSPDQVsPVSEMTSTSLYQDk.[Q] N-Term(TMT6plex); S8(Phospho); K22(TMT6plex) 1 P46821 P46821 0 3 0.1135 0 1 1 974.80091 2922.38817 2922.38441 1.29 0.00125 615713.0625 CID (Collision Induced Dissociation) MS2 0.0 80 20.5 60.0 106.8842 35257 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 17.2 18.6 15.7 21.6 17.7 28.1 19.9 20.5 22.7 23.0 2.82 1 0.0004462 0.0005822 False High Sequest HT (A2) Unambiguous SANEFSTTPEDK [K].sANEFSTtPEDk.[H] N-Term(TMT6plex); T8(Phospho); K12(TMT6plex) 1 Q06315 Q06315 0 3 0.2021 0 1 1 621.96402 1863.87751 1863.87776 -0.13 -8e-05 3007472.25 CID (Collision Induced Dissociation) MS2 1.456394 50 57.6 50.614 65.4961 19730 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 31.8 53.4 80.2 35.6 54.1 83.1 46.0 47.8 87.4 56.4 Excluded by Method 2.82 1 0.0004462 0.001198 False High Sequest HT (A2) Unambiguous DFDLENDEYELSEEEK [K].dFDLENDEYELsEEEk.[N] N-Term(TMT6plex); S12(Phospho); K16(TMT6plex) 1 Q03661 Q03661 0 3 0.3201 0 1 1 848.04338 2542.1156 2542.11621 -0.24 -0.0002 838482.6875 CID (Collision Induced Dissociation) MS2 67.39506 30 20.5 60.0 109.4481 36215 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 10.7 15.3 22.2 13.0 18.6 30.8 14.8 21.2 36.2 22.2 Excluded by Method 2.78 1 0.0004462 0.001066 False High Sequest HT (A2) Unambiguous LFPADTSPSTATK [K].lFPADTsPSTATk.[I] N-Term(TMT6plex); S7(Phospho); K13(TMT6plex) 2 Q6NZY4 Q6NZY4-2; Q6NZY4 0 3 0.0692 0 1 1 625.32875 1873.9717 1873.97127 0.23 0.00015 4213823.0 CID (Collision Induced Dissociation) MS2 4.233155 50 121.5 37.201 95.8465 31148 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 123.3 123.3 103.4 112.3 110.1 128.5 122.6 136.9 121.0 133.1 Excluded by Method 2.89 1 0.0004462 0.001387 False High Sequest HT (A2) Unambiguous ELEAERSLMSSPEDLTK [K].eLEAERSLMssPEDLTk.[D] N-Term(TMT6plex); S10(Phospho); S11(Phospho); K17(TMT6plex) 1 P46821 P46821 1 3 0.357 0 1 1 851.73668 2553.19548 2553.19607 -0.23 -0.0002 2320203.5 CID (Collision Induced Dissociation) MS2 10.20341 30 163.8 54.956 109.2128 36125 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 179.0 163.4 118.9 168.2 157.6 142.9 190.1 184.5 166.2 167.7 Excluded by Method 3.95 1 0.0004462 0.001037 False High Sequest HT (A2) Unambiguous LYERRGGGSGGGEESEGEEVDED [K].lYERRGGGSGGGEEsEGEEVDED.[-] N-Term(TMT6plex); S15(Phospho) 1 Q96QR8 Q96QR8 2 3 0.168 0 1 1 908.04897 2722.13235 2722.13147 0.32 0.00029 259220.296875 CID (Collision Induced Dissociation) MS2 13.9988 60 193.8 60.0 53.0931 15159 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 180.3 199.9 162.1 197.5 193.3 206.1 211.6 202.4 190.0 194.6 Excluded by Method 2.5 1 0.0004462 0.0008258 False High Sequest HT (A2) Unambiguous GELAEDSGDDDDLISLAETTSK [K].gELAEDsGDDDDLISLAETTSk.[K] N-Term(TMT6plex); S7(Phospho); K22(TMT6plex) 1 Q03834 Q03834 0 3 0.3554 0 1 1 940.44086 2819.30803 2819.31235 -1.53 -0.00144 536524.9375 CID (Collision Induced Dissociation) MS2 26.11656 60 52.7 60.0 125.8246 42256 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 16.5 41.6 80.8 16.3 45.4 100.8 16.2 48.2 103.7 57.1 Excluded by Method 4.08 1 0.0004462 0.0006369 False High Sequest HT (A2) Unambiguous EMLASDDEEDVSSK [K].emLAsDDEEDVSSk.[V] N-Term(TMT6plex); M2(Oxidation); S5(Phospho); K14(TMT6plex) 4 Q0ZGT2 Q0ZGT2-2; Q0ZGT2-4; Q0ZGT2; Q0ZGT2-3 0 3 0.5152 0 1 1 703.64948 2108.93389 2108.93468 -0.38 -0.00026 7583808.5 CID (Collision Induced Dissociation) MS2 3.381158 40 70.6 16.338 66.2432 19998 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 72.4 67.9 55.8 77.6 64.7 67.4 76.3 77.2 68.8 78.1 Excluded by Method 3.63 1 0.0004462 0.001265 False High Sequest HT (A2) Unambiguous LFESAQNSPLK [K].lFESAQNsPLk.[Q] N-Term(TMT6plex); S8(Phospho); K11(TMT6plex) 1 P07866 P07866 0 3 0.4451 0 1 1 591.31769 1771.93851 1771.93957 -0.6 -0.00035 380573.15625 CID (Collision Induced Dissociation) MS2 0.0 50 35.3 60.0 98.0161 31968 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 22.9 30.1 42.7 12.2 31.8 59.0 24.0 36.6 62.4 31.8 Excluded by Method 3.37 1 0.0004462 0.002114 False High Sequest HT (A2) Unambiguous AYEELSNTDLEFK [K].aYEELsNTDLEFk.[F] N-Term(TMT6plex); S6(Phospho); K13(TMT6plex) 1 P16521 P16521 0 2 0.0859 0 1 1 1049.01226 2097.01724 2097.01934 -1.0 -0.00105 659205.5625 CID (Collision Induced Dissociation) MS2 26.84127 30 51.2 60.0 109.0625 36067 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 16.4 39.0 72.7 18.2 45.5 100.7 22.4 42.3 102.2 53.0 Excluded by Method 3.84 1 0.0004462 0.000683 False High Sequest HT (A2) Unambiguous LLEPAYDGASVEQQ [K].lLEPAYDGASVEQQ.[-] N-Term(TMT6plex) 1 P17423 P17423 0 2 0.6604 0 1 1 874.94945 1748.89162 1748.89041 0.69 0.00061 814190.875 CID (Collision Induced Dissociation) MS2 18.69927 70 23.9 60.0 96.96 31563 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 9.9 22.0 34.1 12.8 22.0 43.7 13.0 22.6 35.0 24.4 3.21 1 0.0004462 0.0006027 False High Sequest HT (A2) Unambiguous AAEDSPYWVSPAYSK [K].aAEDSPYWVsPAYSk.[T] N-Term(TMT6plex); S10(Phospho); K15(TMT6plex) 1 Q9NW82 Q9NW82 0 3 0.119 0 1 1 737.02556 2209.06213 2209.06187 0.12 9e-05 521281.625 CID (Collision Induced Dissociation) MS2 0.0 50 57.7 60.0 97.6799 31841 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 61.1 52.1 44.6 50.3 62.6 63.5 64.7 58.3 58.6 60.9 Excluded by Method 3.36 1 0.0004462 0.002102 False High Sequest HT (A2) Unambiguous QTGERSIFSQEVVELPDSEDETQQVNK [K].qTGERSIFSQEVVELPDsEDETQQVNk.[T] N-Term(TMT6plex); S18(Phospho); K27(TMT6plex) 1 P53885 P53885 1 3 0.1036 0 1 1 1210.9266 3630.76525 3630.75765 2.09 0.00253 298191.34375 CID (Collision Induced Dissociation) MS2 49.65733 80 2.6 60.0 104.1599 34273 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 1.4 3.3 3.6 2.3 9.3 1.4 2.2 2.5 3.09 1 0.0004462 0.00199 False High Sequest HT (A2) Unambiguous DAGEGGLSLAIEGPSK [K].dAGEGGLSLAIEGPSk.[A] N-Term(TMT6plex); K16(TMT6plex) 2 P21333 P21333; P21333-2 0 3 0.4127 0 1 1 653.69902 1959.0825 1959.07989 1.33 0.00087 421268.25 CID (Collision Induced Dissociation) MS2 24.95099 30 57.3 60.0 95.5356 31085 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 51.1 55.9 48.5 57.1 55.9 58.0 60.3 57.0 63.6 65.7 Excluded by Method 3.32 1 0.0004462 0.0006561 False High Sequest HT (A2) Unambiguous IAALAGQQ [K].iAALAGQQ.[-] N-Term(TMT6plex) 1 P26321 P26321 0 2 0.689 0 1 1 500.80279 1000.5983 1000.59886 -0.56 -0.00028 1106528.125 CID (Collision Induced Dissociation) MS2 18.45841 80 263.6 60.0 75.767 23650 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 52.2 224.8 423.3 77.0 212.0 535.2 83.0 241.2 520.4 266.7 2.54 1 0.0004462 0.001114 False High Sequest HT (A2) Unambiguous YSPKTPPRWAEDQNSLLK [K].ySPktPPRWAEDQNSLLk.[M] N-Term(TMT6plex); Y1(Phospho); K4(TMT6plex); T5(Phospho); K18(TMT6plex) 3 P49792 P49792; A6NKT7; Q7Z3J3 2 4 0.4585 0 1 1 745.13592 2977.52184 2977.51929 0.86 0.00064 892058.5 CID (Collision Induced Dissociation) MS2 0.0 30 113.4 60.0 103.4987 33986 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 127.0 104.1 85.5 135.0 109.9 100.4 133.1 115.1 119.0 104.6 Excluded by Method 4.58 1 0.0004462 0.0005907 False High Sequest HT (A2) Unambiguous TNLSGRQSPSFK [K].tNLSGRQsPSFk.[L] N-Term(TMT6plex); S8(Phospho); K12(TMT6plex) 3 Q6IN85 Q6IN85-2; Q6IN85; Q6IN85-4 1 3 0.2205 0 1 1 620.66373 1859.97664 1859.97808 -0.78 -0.00048 4893494.0 CID (Collision Induced Dissociation) MS2 9.742826 30 149.1 41.305 61.7082 18309 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 142.4 143.7 117.2 167.9 141.3 164.3 158.5 169.1 152.7 133.7 Excluded by Method 2.54 1 0.0004462 0.0008276 False High Sequest HT (A2) Unambiguous EVTPESGPPK [K].eVtPESGPPk.[V] N-Term(TMT6plex); T3(Phospho); K10(TMT6plex) 4 P46087 P46087-3; P46087-2; P46087; P46087-4 0 2 0.2724 0 1 1 789.91286 1578.81845 1578.81806 0.25 0.00019 3232942.0 CID (Collision Induced Dissociation) MS2 4.841933 50 221.6 60.0 56.9331 16585 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 220.1 218.6 188.2 222.0 215.2 220.8 246.1 230.9 223.3 230.7 Excluded by Method 3.01 1 0.0004462 0.0006527 False High Sequest HT (A2) Unambiguous NAEEESESEAEEGD [K].nAEEEsEsEAEEGD.[-] N-Term(TMT6plex); S6(Phospho); S8(Phospho) 4 Q7L1Q6 Q7L1Q6-2; Q7L1Q6-4; Q7L1Q6-3; Q7L1Q6 0 2 0.9233 0 1 1 957.32425 1913.64122 1913.6412 0.01 1e-05 292647.90625 CID (Collision Induced Dissociation) MS2 0.0 50 15.2 60.0 40.866 10680 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 18.2 12.6 10.8 14.2 13.5 15.7 17.0 19.2 18.5 12.3 Excluded by Method 3.0 1 0.0004462 0.001274 False High Sequest HT (A2) Unambiguous SLDEIIGSNK [K].sLDEIIGSNk.[A] N-Term(TMT6plex); S1(Phospho); K10(TMT6plex) 1 Q12159 Q12159 0 3 0.388 0 1 1 538.62269 1613.85352 1613.85517 -1.02 -0.00055 566438.125 CID (Collision Induced Dissociation) MS2 33.96407 90 21.2 60.0 103.3462 33970 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 6.8 19.9 31.2 7.7 15.0 35.1 10.0 18.2 41.6 26.1 2.99 1 0.0004462 0.001327 False High Sequest HT (A2) Unambiguous TRESEPLSK [K].tREsEPLSk.[S] N-Term(TMT6plex); S4(Phospho); K9(TMT6plex) 1 O60669 O60669 1 3 0.306 0 1 1 528.95159 1584.84022 1584.83986 0.23 0.00012 2477381.5 CID (Collision Induced Dissociation) MS2 0.0 30 200.1 60.0 48.757 13550 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 191.1 198.5 164.4 205.7 191.4 212.6 216.5 213.8 200.9 206.1 Excluded by Method 4.02 1 0.0004462 0.0005746 False High Sequest HT (A2) Unambiguous TQSPEFEAQSSK [K].tQsPEFEAQSSk.[F] N-Term(TMT6plex); S3(Phospho); K12(TMT6plex) 1 Q9H5H4 Q9H5H4 0 3 0.0348 0 1 1 626.30782 1876.90892 1876.9094 -0.25 -0.00016 3727858.75 CID (Collision Induced Dissociation) MS2 15.61267 60 64.0 46.158 61.2067 18124 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 70.9 64.8 45.9 69.0 58.8 63.8 62.1 72.4 59.9 71.9 Excluded by Method 2.87 1 0.0004462 0.001223 False High Sequest HT (A2) Unambiguous TESPLENVAELK [K].tEsPLENVAELk.[K] N-Term(TMT6plex); S3(Phospho); K12(TMT6plex) 1 P35732 P35732 0 2 0.155 0 1 1 934.49393 1867.98057 1867.98183 -0.67 -0.00063 341619.15625 CID (Collision Induced Dissociation) MS2 43.68027 30 21.5 60.0 105.4875 34770 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 9.4 17.2 29.1 8.9 20.1 36.4 13.1 22.2 34.1 24.3 Excluded by Method 3.87 1 0.0004462 0.001088 False High Sequest HT (A2) Unambiguous VVDYSQFQESDDADEDYGRDSGPPTK [K].vVDYSQFQESDDADEDYGRDsGPPTk.[K] N-Term(TMT6plex); S21(Phospho); K26(TMT6plex) 1 Q9H1E3 Q9H1E3 1 4 0.0088 0 1 1 865.38922 3458.53505 3458.53134 1.07 0.00093 367046.125 CID (Collision Induced Dissociation) MS2 6.918632 50 12.7 60.0 105.245 34680 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 14.2 10.0 11.5 12.3 13.4 12.6 14.0 11.3 12.6 15.1 Excluded by Method 3.39 1 0.0004462 0.001146 False High Sequest HT (A2) Unambiguous ANTSGDFEK [K].aNTsGDFEk.[D] N-Term(TMT6plex); S4(Phospho); K9(TMT6plex) 7 P49792 P0DJD0; P49792; Q99666; P0DJD1; O14715; A6NKT7; Q7Z3J3 0 2 0.0819 0 1 1 753.86619 1506.7251 1506.72416 0.62 0.00047 1841898.125 CID (Collision Induced Dissociation) MS2 0.0 40 341.5 60.0 60.4879 17850 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 337.0 366.0 280.6 345.8 307.8 347.6 389.3 356.3 338.6 346.4 Excluded by Method 4.03 1 0.0004462 0.0006097 False High Sequest HT (A2) Unambiguous NEDVNIDSNQSDIETDGETEK [K].nEDVNIDSNQsDIETDGETEk.[S] N-Term(TMT6plex); S11(Phospho); K21(TMT6plex) 1 Q06096 Q06096 0 3 0.0665 0 1 1 964.10201 2890.29148 2890.28792 1.23 0.00119 312136.5 CID (Collision Induced Dissociation) MS2 0.0 80 14.3 60.0 75.9724 23730 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 3.5 14.0 23.3 6.7 10.1 25.9 4.1 12.7 30.0 13.1 3.76 1 0.0004462 0.0006911 False High Sequest HT (A2) Unambiguous ENGSGDDLFAGLKK [K].eNGsGDDLFAGLkk.[K] N-Term(TMT6plex); S4(Phospho); K13(TMT6plex); K14(TMT6plex) 1 P09064 P09064 1 3 0.359 0 1 1 740.06206 2218.17162 2218.17238 -0.34 -0.00025 1502884.625 CID (Collision Induced Dissociation) MS2 4.048703 20 48.0 60.0 104.642 34453 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 15.5 39.6 65.7 15.4 44.6 98.3 16.2 44.7 88.9 50.9 Excluded by Method 3.76 1 0.0004462 0.001089 False High Sequest HT (A2) Unambiguous IDGATQSSPAEPK [K].iDGATQSsPAEPk.[S] N-Term(TMT6plex); S8(Phospho); K13(TMT6plex) 3 Q32MZ4 Q32MZ4-2; Q32MZ4; Q32MZ4-3 0 3 0.0616 0 1 1 613.64842 1838.93072 1838.93013 0.32 0.0002 2459306.5 CID (Collision Induced Dissociation) MS2 25.98317 30 204.4 29.907 60.6404 17907 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 203.2 195.6 156.3 207.7 198.4 207.0 231.4 213.0 232.5 199.2 Excluded by Method 3.41 1 0.0004462 0.001381 False High Sequest HT (A2) Unambiguous TIHSGLDIDK [K].tIHsGLDIDk.[V] N-Term(TMT6plex); S4(Phospho); K10(TMT6plex) 1 P39929 P39929 0 3 0.0664 0 1 1 546.2949 1636.87013 1636.87116 -0.63 -0.00034 683812.875 CID (Collision Induced Dissociation) MS2 6.964336 80 40.7 60.0 75.9581 23725 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 14.1 34.1 49.8 16.6 38.2 75.3 23.9 35.6 74.5 44.7 2.26 1 0.0004462 0.001925 False High Sequest HT (A2) Unambiguous VDLENRCQSLTEDLEFRK [K].vDLENRcQsLTEDLEFRk.[S] N-Term(TMT6plex); C7(Carbamidomethyl); S9(Phospho); K18(TMT6plex) 1 P20700 P20700 2 4 0.1273 0 1 1 698.35392 2790.39386 2790.38977 1.47 0.00102 516131.1875 CID (Collision Induced Dissociation) MS2 0.0 20 37.7 60.0 104.4359 34377 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 38.6 35.4 29.5 37.6 32.1 43.5 44.8 38.2 42.5 34.6 Excluded by Method 3.22 1 0.0004462 0.001814 False High Sequest HT (A2) Unambiguous LESESTSPSLEMK [K].lESESTsPSLEmk.[I] N-Term(TMT6plex); S7(Phospho); M12(Oxidation); K13(TMT6plex) 4 Q5VT52 Q5VT52-5; Q5VT52; Q5VT52-2; Q5VT52-3 0 3 0.0582 0 1 1 664.65964 1991.96436 1991.96486 -0.25 -0.00017 2096102.875 CID (Collision Induced Dissociation) MS2 45.86697 30 80.4 60.0 75.9036 23704 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 81.6 84.0 68.0 74.4 71.4 85.6 81.3 90.7 89.4 77.6 Excluded by Method 3.61 1 0.0004462 0.001556 False High Sequest HT (A2) Unambiguous IGEGTYGVVYK [K].iGEGtyGVVYk.[AG] N-Term(TMT6plex); T5(Phospho); Y6(Phospho); K11(TMT6plex) 5 P24941 P24941; P06493; Q00526; P24941-2; P06493-2 0 2 0.3406 0 1 1 902.44033 1803.87339 1803.87354 -0.08 -8e-05 2181922.75 CID (Collision Induced Dissociation) MS2 25.60103 30 200.7 60.0 96.3047 31375 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 209.6 193.8 157.9 234.0 181.9 183.0 230.2 207.7 208.6 200.4 Excluded by Method 4.14 1 0.0004462 0.0008181 False High Sequest HT (A2) Unambiguous LLEPAYDGASVEQQ [K].lLEPAYDGASVEQQ.[-] N-Term(TMT6plex) 1 P17423 P17423 0 2 0.6912 0 1 1 874.9486 1748.88993 1748.89041 -0.28 -0.00024 701045.3125 CID (Collision Induced Dissociation) MS2 53.76442 70 35.7 60.0 97.4985 31823 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 13.1 30.1 44.2 17.0 38.0 65.2 23.1 29.4 64.4 32.2 3.4 1 0.0004462 0.0006222 False High Sequest HT (A2) Unambiguous VDLENRCQSLTEDLEFRK [K].vDLENRcQsLTEDLEFRk.[S] N-Term(TMT6plex); C7(Carbamidomethyl); S9(Phospho); K18(TMT6plex) 1 P20700 P20700 2 4 0.2226 0 1 1 698.35298 2790.3901 2790.38977 0.12 8e-05 1227943.5 CID (Collision Induced Dissociation) MS2 6.730902 20 29.6 60.0 105.4846 34726 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 28.4 24.1 25.3 27.8 28.8 33.2 34.8 29.4 32.0 32.5 Excluded by Method 3.19 1 0.0004462 0.002022 False High Sequest HT (A2) Unambiguous RVADAQIQRETYDSNESDDDVTPSTK [K].rVADAQIQRETYDsNEsDDDVTPSTk.[V] N-Term(TMT6plex); S14(Phospho); S17(Phospho); K26(TMT6plex) 1 P32499 P32499 2 4 0.1536 0 1 1 890.40671 3558.60502 3558.60387 0.32 0.00029 1206596.75 CID (Collision Induced Dissociation) MS2 4.641479 20 48.9 60.0 67.7738 20577 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 10.9 39.7 72.7 14.9 42.2 90.0 23.4 46.6 96.5 52.2 Excluded by Method 3.45 1 0.0004462 0.0008983 False High Sequest HT (A2) Unambiguous ENRNLDNVSPK [K].eNRNLDNVsPk.[D] N-Term(TMT6plex); S9(Phospho); K11(TMT6plex) 6 P78563 P78563-4; P78563; P78563-3; P78563-5; P78563-2; P78563-6 1 3 0.2932 0 1 1 608.65268 1823.94348 1823.9417 0.98 0.00059 508115.53125 CID (Collision Induced Dissociation) MS2 3.870413 20 186.2 60.0 48.8588 13588 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 191.6 187.3 124.2 209.3 185.9 164.8 216.2 192.6 199.3 190.5 Excluded by Method 3.07 1 0.0004462 0.0007284 False High Sequest HT (A2) Unambiguous EKPLNSDQEDVFISEK [K].ekPLNsDQEDVFISEk.[T] N-Term(TMT6plex); K2(TMT6plex); S6(Phospho); K16(TMT6plex) 1 Q04279 Q04279 0 3 0.492 0 1 1 882.46098 2645.3684 2645.36784 0.21 0.00019 844969.8125 CID (Collision Induced Dissociation) MS2 10.1754 30 40.7 60.0 96.3581 31397 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 14.9 33.3 54.2 18.2 32.8 72.5 15.3 40.8 81.9 42.9 Excluded by Method 4.35 1 0.0004462 0.001206 False High Sequest HT (A2) Unambiguous ELAEDVDDASTGDGAAK [K].eLAEDVDDAStGDGAAk.[G] N-Term(TMT6plex); T11(Phospho); K17(TMT6plex) 1 P17890 P17890 0 3 0.0353 0 1 1 734.67879 2202.02182 2202.02152 0.13 0.0001 1373384.5 CID (Collision Induced Dissociation) MS2 0.0 60 31.5 60.0 74.0751 23022 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 11.8 34.4 38.0 22.5 28.3 43.7 21.2 27.3 52.3 35.4 Excluded by Method 3.4 1 0.0004462 0.001307 False High Sequest HT (A2) Unambiguous KDEAIPELVEGQTFDADVE [K].kDEAIPELVEGQTFDADVE.[-] N-Term(TMT6plex); K1(TMT6plex) 1 Q02642 Q02642 1 3 0.2742 0 1 1 855.11136 2563.31954 2563.31795 0.62 0.00053 874250.6875 CID (Collision Induced Dissociation) MS2 0.0 80 50.9 60.0 116.7757 38915 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 15.4 41.7 75.4 15.9 44.1 91.3 22.0 53.9 94.4 54.5 3.1 1 0.0004462 0.001635 False High Sequest HT (A2) Unambiguous DTSTLEENPDILDDDK [K].dtStLEENPDILDDDk.[E] N-Term(TMT6plex); T2(Phospho); T4(Phospho); K16(TMT6plex) 1 P38713 P38713 0 3 0.0376 0 1 1 813.35985 2438.06501 2438.0665 -0.61 -0.0005 530879.625 CID (Collision Induced Dissociation) MS2 27.56735 70 36.7 60.0 104.8584 34494 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 17.5 37.7 45.8 19.8 30.3 56.3 22.6 34.3 71.0 32.2 2.66 1 0.0004462 0.001246 False High Sequest HT (A2) Unambiguous YSPKTPPRWAEDQNSLLK [K].ySPktPPRWAEDQNSLLk.[M] N-Term(TMT6plex); Y1(Phospho); K4(TMT6plex); T5(Phospho); K18(TMT6plex) 3 P49792 P49792; A6NKT7; Q7Z3J3 2 4 0.4684 0 1 1 745.1359 2977.52178 2977.51929 0.84 0.00062 2641946.0 CID (Collision Induced Dissociation) MS2 0.0 20 105.3 60.0 102.6109 33688 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 119.9 110.7 85.9 115.6 99.2 97.8 116.2 112.3 105.2 90.4 Excluded by Method 4.12 1 0.0004462 0.0008838 False High Sequest HT (A2) Unambiguous SSLETIVEK [K].ssLETIVEk.[K] N-Term(TMT6plex); S2(Phospho); K9(TMT6plex) 1 P47035 P47035 0 2 0.1104 0 1 1 772.42277 1543.83826 1543.83846 -0.13 -0.0001 528662.25 CID (Collision Induced Dissociation) MS2 0.0 30 78.0 60.0 96.8526 31582 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 34.2 66.7 91.2 41.1 60.8 138.6 54.1 74.2 136.1 82.6 Excluded by Method 3.08 1 0.0004462 0.001566 False High Sequest HT (A2) Unambiguous RIACEEEFSDSEEEGEGGRK [K].rIAcEEEFsDsEEEGEGGRk.[N] N-Term(TMT6plex); C4(Carbamidomethyl); S9(Phospho); S11(Phospho); K20(TMT6plex) 1 Q13547 Q13547 2 4 0.5149 0 1 1 733.81764 2932.24873 2932.24733 0.48 0.00035 575559.5625 CID (Collision Induced Dissociation) MS2 14.07945 60 57.5 60.0 62.5109 18612 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 54.1 60.3 46.1 56.7 60.6 56.6 58.0 63.2 57.3 62.0 Excluded by Method 4.37 1 0.0004462 0.0007269 False High Sequest HT (A2) Unambiguous SQENLGNPSK [K].sQENLGNPSk.[N] N-Term(TMT6plex); S1(Phospho); K10(TMT6plex) 2 Q9H0X4 Q9H0X4-2; Q9H0X4 0 3 0.4891 0 1 1 537.94304 1611.81456 1611.81437 0.11 6e-05 1415919.375 CID (Collision Induced Dissociation) MS2 0.0 50 59.8 60.0 52.7096 15012 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 56.4 56.4 54.0 58.2 54.6 60.0 65.3 71.8 59.2 61.6 Excluded by Method 4.13 1 0.0004462 0.0005662 False High Sequest HT (A2) Unambiguous EVGYLSDGVPK [K].eVGYLsDGVPk.[L] N-Term(TMT6plex); S6(Phospho); K11(TMT6plex) 1 P46949 P46949 0 2 0.1073 0 1 1 851.44686 1701.88644 1701.88647 -0.02 -2e-05 1732396.0 CID (Collision Induced Dissociation) MS2 25.74827 40 130.4 60.0 89.2351 28717 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 58.0 97.4 178.8 64.7 109.8 210.6 78.7 135.2 243.3 127.9 Excluded by Method 3.54 1 0.0004462 0.0006152 False High Sequest HT (A2) Unambiguous ESEQESEEEILAQK [K].esEQESEEEILAQk.[K] N-Term(TMT6plex); S2(Phospho); K14(TMT6plex) 1 Q9BW71 Q9BW71 0 3 0.0189 0 1 1 729.68526 2187.04123 2187.04701 -2.64 -0.00193 837777.8125 CID (Collision Induced Dissociation) MS2 19.20287 20 148.6 48.123 82.6296 26152 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 159.2 146.1 115.8 157.2 140.3 146.2 169.6 169.2 136.9 145.9 Excluded by Method 4.77 1 0.0004462 0.001066 False High Sequest HT (A2) Unambiguous EVEDKESEGEEEDEDEDLSK [K].eVEDkEsEGEEEDEDEDLSk.[Y] N-Term(TMT6plex); K5(TMT6plex); S7(Phospho); K20(TMT6plex) 2 O95218 O95218-2; O95218 1 3 0.5292 0 1 1 1036.46888 3107.39208 3107.392 0.03 3e-05 377264.78125 CID (Collision Induced Dissociation) MS2 59.50879 40 17.5 60.0 71.7812 22159 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 14.9 29.8 13.7 25.9 12.3 16.8 16.3 17.2 13.1 15.4 Excluded by Method 3.59 1 0.0004462 0.0006092 False High Sequest HT (A2) Unambiguous ESEQESEEEILAQK [K].eSEQEsEEEILAQk.[K] N-Term(TMT6plex); S6(Phospho); K14(TMT6plex) 1 Q9BW71 Q9BW71 0 2 0.1029 0 1 1 1094.02704 2187.04681 2187.04701 -0.09 -0.0001 1233165.875 CID (Collision Induced Dissociation) MS2 0.0 30 10.7 60.0 82.6865 26261 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 9.7 9.4 9.3 7.7 11.2 9.6 16.6 12.0 12.0 9.8 Excluded by Method 3.79 1 0.0004462 0.0009607 False High Sequest HT (A2) Unambiguous WFFISGSV [K].wFFISGsV.[-] N-Term(TMT6plex); S7(Phospho) 1 P00817 P00817 0 2 0.2818 0 1 1 626.30452 1251.60176 1251.60124 0.41 0.00026 535071.6875 CID (Collision Induced Dissociation) MS2 0.0 50 198.7 60.0 132.6945 45199 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 50.2 160.1 311.6 46.5 155.0 386.1 73.3 188.4 428.4 187.2 Excluded by Method 2.91 1 0.0004462 0.001799 False High Sequest HT (A2) Unambiguous ALVLELDEMSNGSEIQDALEEISGQK [K].aLVLELDEmsNGSEIQDALEEISGQk.[T] N-Term(TMT6plex); M9(Oxidation); S10(Phospho); K26(TMT6plex) 2 P17695 P17695; P17695-2 0 4 0.0824 0 1 1 843.92056 3372.66041 3372.65337 2.09 0.00176 832319.3125 CID (Collision Induced Dissociation) MS2 31.42001 50 31.8 42.724 132.6051 45164 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 16.7 21.0 49.7 15.4 29.7 51.9 20.0 30.7 55.5 27.6 Excluded by Method 4.49 1 0.0004462 0.001881 False High Sequest HT (A2) Unambiguous ENGSGDDLFAGLK [K].eNGsGDDLFAGLk.[K] N-Term(TMT6plex); S4(Phospho); K13(TMT6plex) 1 P09064 P09064 0 3 0.4421 0 1 1 620.97641 1860.91469 1860.91448 0.11 7e-05 321277.0 CID (Collision Induced Dissociation) MS2 70.96649 40 251.7 60.0 114.4358 38067 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 61.4 216.1 389.1 82.0 205.6 473.8 83.5 240.6 509.4 255.8 Excluded by Method 3.37 1 0.0004462 0.000566 False High Sequest HT (A2) Unambiguous ETQSPEQVK [K].eTQsPEQVk.[S] N-Term(TMT6plex); S4(Phospho); K9(TMT6plex) 3 Q9NYF8 Q9NYF8-3; Q9NYF8; Q9NYF8-2 0 3 0.0595 0 1 1 528.60781 1583.80887 1583.80822 0.41 0.00021 2026337.0 CID (Collision Induced Dissociation) MS2 3.551791 60 180.6 60.0 47.9075 13192 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 197.4 189.8 152.2 170.3 179.5 172.2 208.3 178.8 190.6 167.1 Excluded by Method 3.36 1 0.0004462 0.0009552 False High Sequest HT (A2) Unambiguous FNDSEGDDTEETEDYRQFRK [K].fNDsEGDDtEETEDYRQFRk.[S] N-Term(TMT6plex); S4(Phospho); T9(Phospho); K20(TMT6plex) 3 Q9NYF8 Q9NYF8-3; Q9NYF8; Q9NYF8-2 2 4 0.4638 0 1 1 775.58081 3099.3014 3099.30221 -0.26 -0.0002 2409745.5 CID (Collision Induced Dissociation) MS2 3.308093 20 54.2 59.301 82.5522 26123 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 52.4 49.4 46.5 57.3 48.3 53.6 57.1 63.3 56.7 57.2 Excluded by Method 3.73 1 0.0004462 0.001489 False High Sequest HT (A2) Unambiguous DSSVDAFEK [K].dSsVDAFEk.[E] N-Term(TMT6plex); S3(Phospho); K9(TMT6plex) 1 P09064 P09064 0 2 0.1368 0 1 1 768.37269 1535.73811 1535.73948 -0.89 -0.00069 3432854.5 CID (Collision Induced Dissociation) MS2 0.0 50 107.2 60.0 75.2006 23355 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 26.1 94.9 156.0 36.9 84.5 199.4 47.4 98.8 219.2 108.4 Excluded by Method 3.8 1 0.0004462 0.0007287 False High Sequest HT (A2) Unambiguous ESEEGNPVRGSEEDSPK [K].eSEEGNPVRGSEEDsPk.[K] N-Term(TMT6plex); S15(Phospho); K17(TMT6plex) 1 O43719 O43719 1 3 0.2991 0 1 1 795.37361 2384.10628 2384.1019 1.84 0.00146 599924.0625 CID (Collision Induced Dissociation) MS2 10.07122 10 102.0 60.0 45.7682 12450 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 96.4 104.5 78.0 117.7 92.1 104.9 123.9 108.2 102.5 91.9 Excluded by Method 3.31 1 0.0004462 0.001465 False High Sequest HT (A2) Unambiguous LRENLNSSPTSEFK [K].lRENLNSsPTSEFk.[S] N-Term(TMT6plex); S8(Phospho); K14(TMT6plex) 1 P13382 P13382 1 3 0.0638 0 1 1 720.70829 2160.11031 2160.11022 0.04 3e-05 1174030.375 CID (Collision Induced Dissociation) MS2 0.0 60 87.4 60.0 81.5099 25728 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 20.4 73.6 135.8 28.2 69.1 172.3 27.5 81.5 177.1 88.3 Excluded by Method 3.45 1 0.0004462 0.0007804 False High Sequest HT (A2) Unambiguous EESTTEATGNESQDATK [K].eESTTEATGNEsQDATk.[V] N-Term(TMT6plex); S12(Phospho); K17(TMT6plex) 1 P32499 P32499 0 3 0.2691 0 1 1 779.35697 2336.05635 2336.05428 0.88 0.00069 808086.1875 CID (Collision Induced Dissociation) MS2 2.375459 90 16.1 60.0 45.7602 12447 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 3.2 11.9 18.9 3.5 15.4 29.2 7.6 16.8 35.5 19.1 3.01 1 0.0004462 0.0009853 False High Sequest HT (A2) Unambiguous ALVLELDEMSNGSEIQDALEEISGQK [K].aLVLELDEmsNGSEIQDALEEISGQk.[T] N-Term(TMT6plex); M9(Oxidation); S10(Phospho); K26(TMT6plex) 2 P17695 P17695; P17695-2 0 4 0.0914 0 1 1 843.92148 3372.66408 3372.65337 3.17 0.00268 307707.4375 CID (Collision Induced Dissociation) MS2 36.46001 50 41.9 60.0 136.0529 46396 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 25.2 30.4 57.7 16.5 34.7 63.8 27.1 43.8 73.6 45.9 Excluded by Method 3.61 1 0.0004462 0.0009892 False High Sequest HT (A2) Unambiguous AANMSDDESSLIK [K].aANmsDDESSLIk.[N] N-Term(TMT6plex); M4(Oxidation); S5(Phospho); K13(TMT6plex) 1 P19880 P19880 0 3 0.4504 0 1 1 645.64379 1934.91681 1934.91825 -0.74 -0.00048 1824313.0 CID (Collision Induced Dissociation) MS2 31.09868 30 57.1 60.0 75.2071 23358 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 14.2 46.7 84.4 19.0 43.5 107.9 19.6 56.0 125.7 53.5 Excluded by Method 2.62 1 0.0004462 0.000638 False High Sequest HT (A2) Unambiguous SRSPGSPVGEGTGSPPK [K].sRSPGsPVGEGTGSPPk.[W] N-Term(TMT6plex); S6(Phospho); K17(TMT6plex) 6 P35611 P35611-2; P35611-6; P35611-4; P35611-3; P35611-5; P35611 1 4 0.1278 0 1 1 534.52839 2135.09173 2135.08982 0.89 0.00048 1109996.0 CID (Collision Induced Dissociation) MS2 0.0 60 76.3 60.0 47.4545 13024 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 76.0 84.3 64.7 78.9 77.4 71.2 74.2 79.7 78.7 77.9 Excluded by Method 2.66 1 0.0004462 0.0007125 False High Sequest HT (A2) Unambiguous LPWEVSDGEVSSGK [K].lPWEVSDGEVsSGk.[T] N-Term(TMT6plex); S11(Phospho); K14(TMT6plex) 1 P48415 P48415 0 3 0.0683 0 1 1 676.67462 2028.00929 2028.00911 0.09 6e-05 762291.375 CID (Collision Induced Dissociation) MS2 5.643403 30 88.7 60.0 114.2488 37997 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 78.9 86.3 77.3 89.1 76.1 99.4 94.2 97.6 102.2 86.1 Excluded by Method 3.22 1 0.0004462 0.0007673 False High Sequest HT (A2) Unambiguous SNVPESINEDISK [K].sNVPEsINEDISk.[T] N-Term(TMT6plex); S6(Phospho); K13(TMT6plex) 1 Q08412 Q08412 0 3 0.1682 0 1 1 657.33456 1969.98914 1969.98837 0.39 0.00025 1533446.25 CID (Collision Induced Dissociation) MS2 3.027233 30 88.3 60.0 85.0233 27057 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 21.1 70.8 128.3 25.3 72.2 160.0 31.3 84.7 195.5 93.4 Excluded by Method 3.27 1 0.0004462 0.0008691 False High Sequest HT (A2) Unambiguous LSNSSLSNLFK [K].lSNsSLSNLFk.[K] N-Term(TMT6plex); S4(Phospho); K11(TMT6plex) 1 P13259 P13259 0 3 0.0068 0 1 1 583.31758 1747.93818 1747.93957 -0.8 -0.00046 2241791.0 CID (Collision Induced Dissociation) MS2 31.93938 50 166.6 46.985 127.6142 42985 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 40.0 155.1 245.4 49.8 126.6 327.4 64.7 153.6 334.8 169.1 Excluded by Method 2.94 1 0.0004462 0.001197 False High Sequest HT (A2) Unambiguous VLLVLCGGDD [K].vLLVLcGGDD.[-] N-Term(TMT6plex); C6(Carbamidomethyl) 3 P09525 P09525-2; P09525; P09525-3 0 2 0.5267 0 1 1 645.35186 1289.69644 1289.69725 -0.63 -0.00041 807505.125 CID (Collision Induced Dissociation) MS2 26.49066 80 96.8 60.0 126.5873 42570 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 80.4 86.2 105.6 82.6 92.5 115.5 85.2 107.5 119.5 93.5 2.43 1 0.0004462 0.001025 False High Sequest HT (A2) Unambiguous LNELQNSEDSDAEDGGK [K].lNELQNsEDsDAEDGGk.[Q] N-Term(TMT6plex); S7(Phospho); S10(Phospho); K17(TMT6plex) 1 Q12136 Q12136 0 3 0.5573 0 1 1 813.68548 2439.04187 2439.0366 2.16 0.00176 298065.46875 CID (Collision Induced Dissociation) MS2 24.24961 60 37.2 60.0 83.8742 26624 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 20.5 34.4 50.3 23.5 28.6 60.3 25.7 33.3 57.2 38.5 Excluded by Method 3.75 1 0.0004462 0.0005785 False High Sequest HT (A2) Unambiguous TEWLDGK [K].tEWLDGk.[H] N-Term(TMT6plex); K7(TMT6plex) 8 P62937 A0A0B4J2A2; F5H284; Q9Y536; P0DN26; P62937; A0A075B767; P0DN37; P62937-2 0 2 0.3843 0 1 1 653.87388 1306.74047 1306.74072 -0.19 -0.00012 1254312.625 CID (Collision Induced Dissociation) MS2 5.851671 80 84.6 60.0 84.2287 26758 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 77.7 84.2 70.2 82.1 77.6 78.4 97.2 98.3 90.4 89.9 2.42 1 0.0004462 0.002107 False High Sequest HT (A2) Unambiguous LEDIPVDDIDFSDLEEQYK [K].lEDIPVDDIDFsDLEEQYk.[V] N-Term(TMT6plex); S12(Phospho); K19(TMT6plex) 1 P06103 P06103 0 3 0.3938 0 1 1 941.1202 2821.34604 2821.34727 -0.44 -0.00041 2946459.0 CID (Collision Induced Dissociation) MS2 28.17959 60 16.7 24.82 129.8395 44001 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 4.2 11.9 24.3 7.1 16.5 29.5 7.2 19.8 29.9 16.7 Excluded by Method 4.52 1 0.0004462 0.0006014 False High Sequest HT (A2) Unambiguous ESVENNSEEGFIK [K].eSVENNsEEGFIk.[V] N-Term(TMT6plex); S7(Phospho); K13(TMT6plex) 1 P36029 P36029 0 3 0.0223 0 1 1 673.99361 2019.96629 2019.96764 -0.67 -0.00045 1270973.75 CID (Collision Induced Dissociation) MS2 13.90909 50 50.1 53.307 83.7779 26676 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 14.6 40.7 74.0 13.3 37.0 93.1 21.1 53.2 92.8 61.1 Excluded by Method 3.59 1 0.0004462 0.001247 False High Sequest HT (A2) Unambiguous LCAGIMITASHNPK [K].lcAGImITAsHNPk.[Q] N-Term(TMT6plex); C2(Carbamidomethyl); M6(Oxidation); S10(Phospho); K14(TMT6plex) 2 Q96G03 Q96G03-2; Q96G03 0 4 0.1176 0 1 1 517.51872 2067.05303 2067.05324 -0.1 -5e-05 972167.5 CID (Collision Induced Dissociation) MS2 35.28511 70 46.9 60.0 84.4469 26838 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 37.4 44.6 48.9 27.8 36.1 63.5 46.5 48.7 66.9 48.6 2.72 1 0.0004462 0.001259 False High Sequest HT (A2) Unambiguous LVSWYDNEYGYSTRVVDLVEHVAK [K].lVSWYDNEYGYSTRVVDLVEHVAk.[A] N-Term(TMT6plex); K24(TMT6plex) 2 P00359 P00359; P00358 1 4 0.2455 0 1 1 825.93804 3300.73033 3300.7305 -0.05 -4e-05 15936651.0 CID (Collision Induced Dissociation) MS2 0.0 50 11.9 4.856 128.499 43396 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 2.4 20.7 12.7 1.5 5.5 24.3 13.6 20.6 18.0 Excluded by Method 2.77 1 0.0004462 0.001529 False High Sequest HT (A2) Unambiguous DISASSPNLGGILK [K].dISASsPNLGGILk.[A] N-Term(TMT6plex); S6(Phospho); K14(TMT6plex) 1 Q08548 Q08548 0 3 0.0139 0 1 1 637.35041 1910.03667 1910.04002 -1.75 -0.00112 562528.375 CID (Collision Induced Dissociation) MS2 8.64098 40 174.9 60.0 115.1003 38308 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 44.1 151.1 301.2 59.6 143.3 322.3 60.0 157.9 343.3 166.1 Excluded by Method 4.32 1 0.0004462 0.001737 False High Sequest HT (A2) Unambiguous IMATPEQVGK [K].imAtPEQVGk.[M] N-Term(TMT6plex); M2(Oxidation); T4(Phospho); K10(TMT6plex) 2 P12956 P12956; P12956-2 0 3 0.5559 0 1 1 543.2891 1627.85275 1627.85307 -0.2 -0.00011 1526180.125 CID (Collision Induced Dissociation) MS2 58.44724 30 156.8 46.044 69.1141 21150 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 153.6 159.0 132.5 148.0 151.4 168.6 170.0 160.6 154.2 170.1 Excluded by Method 2.86 1 0.0004462 0.001623 False High Sequest HT (A2) Unambiguous LEETKESLQNK [K].lEETkEsLQNk.[G] N-Term(TMT6plex); K5(TMT6plex); S7(Phospho); K11(TMT6plex) 1 Q08245 Q08245 1 3 0.1978 0 1 1 696.05135 2086.1395 2086.14001 -0.25 -0.00017 3105233.0 CID (Collision Induced Dissociation) MS2 14.06608 10 203.5 49.176 75.5618 23494 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 50.5 174.6 295.0 68.9 166.5 364.9 79.6 185.6 427.9 221.6 Excluded by Method 4.5 1 0.0004462 0.0006094 False High Sequest HT (A2) Unambiguous SSSPSASLTENEVK [K].sSSPsASLTENEVk.[-] N-Term(TMT6plex); S5(Phospho); K14(TMT6plex) 1 O96028-5 O96028-5 0 3 0.0568 0 1 1 658.66795 1973.98928 1973.98329 3.04 0.002 716706.0625 CID (Collision Induced Dissociation) MS2 0.0 40 90.6 60.0 69.9848 21483 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 84.9 79.9 73.7 95.7 83.5 88.9 107.1 101.1 97.7 93.7 Excluded by Method 4.93 1 0.0004462 0.001118 False High Sequest HT (A2) Unambiguous IAALAGQQ [K].iAALAGQQ.[-] N-Term(TMT6plex) 1 P26321 P26321 0 2 0.6275 0 1 1 500.80293 1000.59857 1000.59886 -0.29 -0.00014 2984213.75 CID (Collision Induced Dissociation) MS2 0.0 60 227.0 60.0 76.2945 23772 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 58.2 171.5 366.7 70.7 179.3 436.7 100.6 200.8 450.1 235.3 Excluded by Method 2.47 1 0.0004462 0.000592 False High Sequest HT (A2) Unambiguous TVSPTTQISAGK [K].tVsPTTQISAGk.[S] N-Term(TMT6plex); S3(Phospho); K12(TMT6plex) 1 P53297 P53297 0 3 0.0313 0 1 1 576.64978 1727.9348 1727.93449 0.18 0.0001 1812307.875 CID (Collision Induced Dissociation) MS2 0.0 80 41.8 60.0 70.8926 21822 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 14.8 31.6 60.8 15.7 36.5 76.8 24.5 34.9 80.0 42.4 2.56 1 0.0004462 0.0009224 False High Sequest HT (A2) Unambiguous NIILEEGK [K].nIILEEGk.[E] N-Term(TMT6plex); K8(TMT6plex) 1 P23528 P23528 0 2 0.1966 0 1 1 687.4239 1373.84052 1373.84044 0.06 4e-05 842377.0 CID (Collision Induced Dissociation) MS2 0.0 100 105.7 60.0 80.5695 25457 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 105.6 109.4 81.7 110.2 96.1 104.4 116.0 104.3 122.7 106.9 2.95 1 0.0004462 0.001718 False High Sequest HT (A2) Unambiguous NAEEESESEAEEGD [K].nAEEEsESEAEEGD.[-] N-Term(TMT6plex); S6(Phospho) 4 Q7L1Q6 Q7L1Q6-2; Q7L1Q6-4; Q7L1Q6-3; Q7L1Q6 0 2 0.1857 0 1 1 917.34142 1833.67555 1833.67487 0.37 0.00034 123995.3515625 CID (Collision Induced Dissociation) MS2 29.66531 90 69.3 60.0 37.3314 9352 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 65.0 72.6 57.3 68.5 68.3 66.4 78.2 71.2 76.6 69.4 3.77 1 0.0004462 0.0008359 False High Sequest HT (A2) Unambiguous IVFDDEVSIENK [K].iVFDDEVsIENk.[E] N-Term(TMT6plex); S8(Phospho); K12(TMT6plex) 1 P46669 P46669 0 3 0.1746 0 1 1 649.33659 1945.99522 1945.9924 1.45 0.00094 790695.8125 CID (Collision Induced Dissociation) MS2 53.41162 40 59.8 60.0 114.8745 38224 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 17.4 52.0 82.5 25.1 49.3 114.4 20.3 55.0 116.2 65.5 Excluded by Method 3.55 1 0.0004462 0.002066 False High Sequest HT (A2) Unambiguous TTSPPEVSGYSYEK [K].tTsPPEVSGYSYEk.[T] N-Term(TMT6plex); S3(Phospho); K14(TMT6plex) 1 P46821 P46821 0 2 0.0557 0 1 1 1042.00684 2083.00639 2083.00369 1.3 0.00135 863300.9375 CID (Collision Induced Dissociation) MS2 0.0 30 123.9 60.0 80.4202 25400 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 121.8 119.3 101.4 127.7 109.9 135.6 146.4 128.5 126.6 121.3 Excluded by Method 3.23 1 0.0004462 0.0006809 False High Sequest HT (A2) Unambiguous TPSADSLPLSNSSTDLTISDNIK [K].tPSADSLPLsNSSTDLTISDNIk.[E] N-Term(TMT6plex); S10(Phospho); K23(TMT6plex) 1 P32525 P32525 0 3 0.0359 0 1 1 972.16187 2914.47107 2914.46985 0.42 0.00041 730452.625 CID (Collision Induced Dissociation) MS2 19.30208 70 44.0 60.0 119.0628 39770 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 14.7 34.9 64.1 15.3 39.2 78.5 19.1 43.5 77.6 52.8 2.51 1 0.0004462 0.001415 False High Sequest HT (A2) Unambiguous TASQIALNESAK [K].tASQIALNESAk.[S] N-Term(TMT6plex); T1(Phospho); K12(TMT6plex) 1 P38742 P38742 0 3 0.061 0 1 1 590.98452 1770.93902 1770.9403 -0.72 -0.00043 529508.375 CID (Collision Induced Dissociation) MS2 13.16467 60 44.1 60.0 76.7585 23946 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 10.7 34.2 61.4 18.5 38.2 82.1 18.5 47.5 80.3 49.6 Excluded by Method 3.28 1 0.0004462 0.00114 False High Sequest HT (A2) Unambiguous DWRGGRTASGNIIPSSTGAAK [K].dWRGGRTAsGNIIPSSTGAAk.[A] N-Term(TMT6plex); S9(Phospho); K21(TMT6plex) 3 P00359 P00360; P00359; P00358 2 4 0.0588 0 1 1 660.84702 2640.36625 2640.36593 0.12 8e-05 2993883.25 CID (Collision Induced Dissociation) MS2 1.645252 20 79.3 33.489 70.5725 21699 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 18.7 67.6 130.2 23.4 73.8 154.4 28.4 77.0 143.6 76.0 Excluded by Method 4.59 1 0.0004462 0.0006226 False High Sequest HT (A2) Unambiguous VFDDESDEK [K].vFDDEsDEk.[E] N-Term(TMT6plex); S6(Phospho); K9(TMT6plex) 1 O43719 O43719 0 2 0.2986 0 1 1 811.37408 1621.74088 1621.73987 0.62 0.0005 930910.75 CID (Collision Induced Dissociation) MS2 31.28778 40 56.9 60.0 76.5543 23871 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 53.9 55.0 52.8 52.1 46.4 71.8 56.3 61.5 63.5 55.7 Excluded by Method 3.55 1 0.0004462 0.001241 False High Sequest HT (A2) Unambiguous ESSSEDLLK [K].eSsSEDLLk.[R] N-Term(TMT6plex); S3(Phospho); K9(TMT6plex) 1 P47048 P47048 0 2 0.1133 0 1 1 773.39356 1545.77984 1545.78134 -0.97 -0.00075 2551256.0 CID (Collision Induced Dissociation) MS2 24.10292 50 202.3 39.891 77.95 24392 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 47.8 168.3 295.4 64.2 171.5 371.1 74.8 186.7 418.1 225.0 Excluded by Method 3.62 1 0.0004462 0.0007706 False High Sequest HT (A2) Unambiguous SASPEVSEGHENQHGQESEAK [K].sAsPEVSEGHENQHGQESEAk.[A] N-Term(TMT6plex); S1(Phospho); S3(Phospho); K21(TMT6plex) 1 O60930 O60930 0 4 0.3664 0 1 1 714.56285 2855.22958 2855.22865 0.33 0.00023 395981.875 CID (Collision Induced Dissociation) MS2 35.34748 60 10.5 60.0 40.4659 10489 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 11.1 10.5 4.1 10.1 10.6 14.6 9.7 12.5 7.8 13.7 Excluded by Method 3.33 1 0.0004462 0.0007619 False High Sequest HT (A2) Unambiguous NAEEESESEAEEGD [K].nAEEEsEsEAEEGD.[-] N-Term(TMT6plex); S6(Phospho); S8(Phospho) 4 Q7L1Q6 Q7L1Q6-2; Q7L1Q6-4; Q7L1Q6-3; Q7L1Q6 0 2 0.8762 0 1 1 957.32389 1913.6405 1913.6412 -0.37 -0.00035 533058.5625 CID (Collision Induced Dissociation) MS2 0.0 40 26.3 60.0 39.036 9970 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 28.3 30.4 22.4 22.4 18.9 21.7 31.4 30.0 33.3 24.5 Excluded by Method 3.15 1 0.0004462 0.001456 False High Sequest HT (A2) Unambiguous SLGSASPGPGQPPLSSPTRGGVK [K].sLGSASPGPGQPPLSsPTRGGVk.[K] N-Term(TMT6plex); S16(Phospho); K23(TMT6plex) 2 Q9C0B5 Q9C0B5; Q9C0B5-2 1 4 0.0057 0 1 1 668.85987 2672.41766 2672.4173 0.13 9e-05 658825.75 CID (Collision Induced Dissociation) MS2 6.264158 40 71.9 60.0 76.382 23806 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 64.9 79.9 58.5 77.2 73.2 80.0 76.4 68.6 73.0 66.8 Excluded by Method 3.51 1 0.0004462 0.0005903 False High Sequest HT (A2) Unambiguous EPSRERDIAK [K].ePsRERDIAk.[E] N-Term(TMT6plex); S3(Phospho); K10(TMT6plex) 2 Q8NI27 Q8NI27; Q8NI27-2 2 3 0.0386 0 1 1 580.31334 1738.92547 1738.92532 0.09 5e-05 4558322.5 CID (Collision Induced Dissociation) MS2 3.539566 30 133.4 49.051 41.8742 10993 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 123.5 130.6 99.2 141.7 125.4 132.0 150.5 150.2 139.3 141.4 Excluded by Method 2.59 1 0.0004462 0.002135 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVSsDEEF.[-] N-Term(TMT6plex); S9(Phospho) 1 P16521 P16521 0 2 0.0074 0 1 1 885.37241 1769.73754 1769.73562 1.08 0.00096 239945.875 CID (Collision Induced Dissociation) MS2 19.71886 60 30.2 60.0 144.2815 49242 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 8.9 24.2 47.5 8.1 28.8 61.1 10.2 26.3 56.7 30.0 Excluded by Method 2.69 1 0.0004462 0.001952 False High Sequest HT (A2) Unambiguous YAAAAPAATSAASGDAAPAEEAAAEEEEESDDDMGFGLFD [K].yAAAAPAATSAASGDAAPAEEAAAEEEEEsDDDmGFGLFD.[-] N-Term(TMT6plex); S30(Phospho); M34(Oxidation) 1 P05317 P05317 0 4 0.25 0 1 1 1062.19316 4245.7508 4245.73286 4.22 0.00448 526913.375 CID (Collision Induced Dissociation) MS2 3.885879 50 16.8 60.0 143.991 49140 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 2.5 15.1 27.1 2.5 11.6 32.5 4.1 17.1 34.6 21.4 Excluded by Method 4.76 1 0.0004462 0.0006775 False High Sequest HT (A2) Unambiguous VQGEAVSNIQENTQTPTVQEESEEEEVDETGVEVK [K].vQGEAVSNIQENTQTPTVQEEsEEEEVDETGVEVk.[D] N-Term(TMT6plex); S22(Phospho); K35(TMT6plex) 3 Q13765 Q13765; E9PAV3; E9PAV3-2 0 4 0.0294 0 1 1 1100.5194 4399.05577 4399.06016 -1.0 -0.0011 199643.53125 CID (Collision Induced Dissociation) MS2 0.0 30 6.8 60.0 143.9249 49118 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 8.0 9.1 7.1 6.8 7.2 7.4 3.8 7.2 9.0 2.8 Excluded by Method 3.4 1 0.0004462 0.001619 False High Sequest HT (A2) Unambiguous EEEAAEESDDDMGFGLFD [K].eEEAAEEsDDDMGFGLFD.[-] N-Term(TMT6plex); S8(Phospho) 2 P05319 P10622; P05319 0 2 0.797 0 1 1 1157.94315 2314.87902 2314.8784 0.27 0.00031 188638.453125 CID (Collision Induced Dissociation) MS2 0.0 50 36.0 60.0 146.0796 49866 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 9.0 36.7 54.0 12.8 30.4 69.1 11.1 36.0 57.4 43.7 Excluded by Method 3.3 1 0.0004462 0.0007709 False High Sequest HT (A2) Unambiguous YAAAAPAATSAASGDAAPAEEAAAEEEEESDDDMGFGLFD [K].yAAAAPAATSAASGDAAPAEEAAAEEEEEsDDDmGFGLFD.[-] N-Term(TMT6plex); S30(Phospho); M34(Oxidation) 1 P05317 P05317 0 4 0.3646 0 1 1 1062.19316 4245.7508 4245.73286 4.22 0.00448 603038.0625 CID (Collision Induced Dissociation) MS2 4.073675 40 10.0 60.0 141.9024 48415 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 1.5 8.9 15.7 2.3 8.1 19.1 2.2 8.3 23.7 10.5 Excluded by Method 4.91 1 0.0004462 0.0009689 False High Sequest HT (A2) Unambiguous TTSPPEVSGYSYEK [K].tTsPPEVSGYSYEk.[T] N-Term(TMT6plex); S3(Phospho); K14(TMT6plex) 1 P46821 P46821 0 2 0.0868 0 1 1 1042.00495 2083.00262 2083.00369 -0.52 -0.00054 617406.25 CID (Collision Induced Dissociation) MS2 0.0 30 114.7 60.0 80.5735 25374 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 115.6 110.2 98.8 115.9 109.3 117.7 133.1 127.3 111.4 108.1 Excluded by Method 3.11 1 0.0004462 0.0008133 False High Sequest HT (A2) Unambiguous ALVLELDEMSNGSEIQDALEEISGQK [K].aLVLELDEmsNGSEIQDALEEISGQk.[T] N-Term(TMT6plex); M9(Oxidation); S10(Phospho); K26(TMT6plex) 2 P17695 P17695; P17695-2 0 3 0.0573 0 1 1 1124.89168 3372.66049 3372.65337 2.11 0.00237 272208.03125 CID (Collision Induced Dissociation) MS2 9.667855 80 11.4 60.0 146.4794 50001 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 6.4 9.4 17.0 7.7 9.0 21.6 3.3 9.0 18.5 12.3 2.79 1 0.0004462 0.0006881 False High Sequest HT (A2) Unambiguous LAALESPPPVLK [K].lAALEsPPPVLk.[A] N-Term(TMT6plex); S6(Phospho); K12(TMT6plex) 1 P21576 P21576 0 3 0.3585 0 1 1 591.68295 1773.03429 1773.03275 0.87 0.00052 285320.25 CID (Collision Induced Dissociation) MS2 28.86805 70 77.7 60.0 120.2571 40212 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 21.8 58.3 124.4 19.9 68.8 160.5 27.8 74.3 146.7 74.0 3.71 1 0.0004462 0.0008644 False High Sequest HT (A2) Unambiguous DHNSEDEDEDK [K].dHNsEDEDEDk.[Y] N-Term(TMT6plex); S4(Phospho); K11(TMT6plex) 1 O95391 O95391 0 3 0.5363 0 1 1 624.26315 1870.7749 1870.77442 0.26 0.00016 142986.875 CID (Collision Induced Dissociation) MS2 26.52083 60 26.1 60.0 29.142 6464 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 26.1 20.1 18.7 31.4 25.9 21.0 39.4 30.6 20.6 27.0 Excluded by Method 3.86 1 0.0004462 0.0005887 False High Sequest HT (A2) Unambiguous EEEAAEESDDDMGFGLFD [K].eEEAAEEsDDDMGFGLFD.[-] N-Term(TMT6plex); S8(Phospho) 2 P05319 P10622; P05319 0 3 0.7703 0 1 1 772.29975 2314.88469 2314.8784 2.72 0.0021 170314.875 CID (Collision Induced Dissociation) MS2 9.338046 80 15.5 60.0 148.5076 50699 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 2.2 15.5 25.0 6.5 9.0 29.0 3.3 14.4 30.0 19.6 3.44 1 0.0004462 0.001758 False High Sequest HT (A2) Unambiguous SFNYSPNSSTSEVSSTSASK [K].sFNYSPNSsTSEVSSTSASk.[A] N-Term(TMT6plex); S9(Phospho); K20(TMT6plex) 4 Q5VT52 Q5VT52-5; Q5VT52; Q5VT52-2; Q5VT52-3 0 3 0.0134 0 1 1 869.07325 2605.20521 2605.20709 -0.72 -0.00063 2280459.25 CID (Collision Induced Dissociation) MS2 3.177699 50 48.2 56.595 80.1784 25225 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 52.7 47.0 38.5 50.3 39.3 49.1 56.0 57.5 48.1 43.4 Excluded by Method 3.74 1 0.0004462 0.0007239 False High Sequest HT (A2) Unambiguous EEEAAEESDDDMGFGLFD [K].eEEAAEEsDDDMGFGLFD.[-] N-Term(TMT6plex); S8(Phospho) 2 P05319 P10622; P05319 0 3 0.769 0 1 1 772.29993 2314.88524 2314.8784 2.95 0.00228 399065.09375 CID (Collision Induced Dissociation) MS2 47.51471 60 26.8 60.0 147.429 50326 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 8.0 20.6 46.0 7.7 24.6 51.3 11.4 24.4 48.0 26.0 Excluded by Method 3.68 1 0.0004462 0.0009753 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVssDEEF.[-] N-Term(TMT6plex); S8(Phospho); S9(Phospho) 1 P16521 P16521 0 2 0.1529 0 1 1 925.35459 1849.70191 1849.70195 -0.02 -2e-05 825904.75 CID (Collision Induced Dissociation) MS2 8.574799 40 76.9 60.0 146.6866 50073 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 18.6 62.3 125.8 18.6 62.3 144.8 19.1 75.3 153.8 88.8 Excluded by Method 3.27 1 0.0004462 0.0006542 False High Sequest HT (A2) Unambiguous YVESDEDDQ [K].yVEsDEDDQ.[-] N-Term(TMT6plex); S4(Phospho) 1 Q02208 Q02208 0 2 0.3444 0 1 1 704.77167 1408.53607 1408.53547 0.43 0.0003 603162.4375 CID (Collision Induced Dissociation) MS2 0.0 50 41.0 60.0 50.0882 13992 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 14.1 27.5 62.4 15.4 39.6 71.7 20.0 39.9 76.6 42.5 Excluded by Method 3.02 1 0.0004462 0.0008893 False High Sequest HT (A2) Unambiguous EVVKPVPITSPAVSK [K].eVVkPVPITsPAVSk.[V] N-Term(TMT6plex); K4(TMT6plex); S10(Phospho); K15(TMT6plex) 1 Q96HC4 Q96HC4 0 3 0.0213 0 1 1 773.46155 2318.3701 2318.37035 -0.11 -8e-05 5679163.5 CID (Collision Induced Dissociation) MS2 1.81936 60 115.1 19.754 91.1291 29347 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 120.1 109.9 91.0 112.8 123.0 122.6 116.9 119.1 107.5 128.1 Excluded by Method 4.22 1 0.0004462 0.0005868 False High Sequest HT (A2) Unambiguous TFIDLESSSPELK [K].tFIDLESSsPELk.[A] N-Term(TMT6plex); S9(Phospho); K13(TMT6plex) 1 P48164 P48164 0 3 0.093 0 1 1 668.68337 2004.03555 2004.03426 0.65 0.00043 982929.0625 CID (Collision Induced Dissociation) MS2 9.950493 40 37.8 60.0 117.1155 39082 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 14.3 27.4 48.2 17.6 35.0 67.3 17.5 37.3 73.0 40.4 Excluded by Method 4.41 1 0.0004462 0.0006157 False High Sequest HT (A2) Unambiguous EYVPLDNAEQSTSSSQETK [K].eYVPLDNAEQSTSssQETk.[E] N-Term(TMT6plex); S14(Phospho); S15(Phospho); K19(TMT6plex) 1 P33322 P33322 0 3 0.0029 0 1 1 911.07586 2731.21304 2731.21529 -0.82 -0.00075 1042795.75 CID (Collision Induced Dissociation) MS2 0.0 40 35.0 60.0 91.204 29376 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 10.0 31.0 51.3 15.0 31.7 56.9 14.2 32.9 68.4 38.4 Excluded by Method 3.42 1 0.0004462 0.0007046 False High Sequest HT (A2) Unambiguous FVDDSDTSYEEK [K].fVDDsDTsYEEk.[E] N-Term(TMT6plex); S5(Phospho); S8(Phospho); K12(TMT6plex) 1 Q08951 Q08951 0 3 0.0657 0 1 1 684.95451 2052.84897 2052.84924 -0.13 -9e-05 793117.6875 CID (Collision Induced Dissociation) MS2 8.11122 30 74.3 60.0 91.3392 29428 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 25.0 60.9 108.8 28.8 63.9 133.0 34.3 66.5 150.6 71.1 Excluded by Method 2.74 1 0.0004462 0.001661 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVssDEEF.[-] N-Term(TMT6plex); S8(Phospho); S9(Phospho) 1 P16521 P16521 0 2 0.1479 0 1 1 925.3549 1849.70252 1849.70195 0.31 0.00028 2812598.0 CID (Collision Induced Dissociation) MS2 0.0 30 111.4 60.0 116.9033 39002 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 30.0 90.7 193.0 31.7 96.3 199.8 37.0 100.8 226.5 108.1 Excluded by Method 3.65 1 0.0004462 0.001308 False High Sequest HT (A2) Unambiguous VDSPDDPFMTK [K].vDsPDDPFmTk.[S] N-Term(TMT6plex); S3(Phospho); M9(Oxidation); K11(TMT6plex) 1 P19659 P19659 0 3 0.4454 0 1 1 602.61918 1805.84298 1805.84329 -0.17 -0.0001 717984.6875 CID (Collision Induced Dissociation) MS2 4.056313 60 79.5 60.0 88.4437 28421 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 17.5 65.3 105.6 27.1 59.4 153.6 32.4 65.2 179.6 89.1 Excluded by Method 3.39 1 0.0004462 0.0005947 False High Sequest HT (A2) Unambiguous DVTPPPETEVVLIK [K].dVtPPPETEVVLIk.[N] N-Term(TMT6plex); T3(Phospho); K14(TMT6plex) 3 P27816 P27816; P27816-6; P27816-2 0 3 0.2021 0 1 1 692.38634 2075.14447 2075.14415 0.16 0.00011 15718789.0 CID (Collision Induced Dissociation) MS2 0.8049264 20 128.0 9.672 116.6996 38927 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 120.0 125.6 97.8 127.2 120.8 135.1 145.5 140.1 128.8 139.5 Excluded by Method 3.86 1 0.0004462 0.001587 False High Sequest HT (A2) Unambiguous EEEAKEESDDDMGFGLFD [K].eEEAkEEsDDDmGFGLFD.[-] N-Term(TMT6plex); K5(TMT6plex); S8(Phospho); M12(Oxidation) 1 P02400 P02400 1 3 0.6196 0 1 1 873.03624 2617.09418 2617.0941 0.03 3e-05 4064673.0 CID (Collision Induced Dissociation) MS2 1.127962 40 168.4 23.331 116.617 38899 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 39.2 135.1 249.4 59.3 140.6 315.7 59.3 145.5 363.8 176.1 Excluded by Method 3.47 1 0.0004462 0.001427 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVSsDEEF.[-] N-Term(TMT6plex); S9(Phospho) 1 P16521 P16521 0 2 0.0655 0 1 1 885.37131 1769.73534 1769.73562 -0.16 -0.00014 1624410.125 CID (Collision Induced Dissociation) MS2 19.33958 30 83.9 55.902 116.2501 38762 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 18.1 64.7 128.8 31.9 67.7 164.3 24.8 83.0 170.6 84.8 Excluded by Method 3.97 1 0.0004462 0.001015 False High Sequest HT (A2) Unambiguous AFSIIPSQRFIK [K].aFSIIPsQRFIk.[S] N-Term(TMT6plex); S7(Phospho); K12(TMT6plex) 1 P38970 P38970 1 3 0.4767 0 1 1 649.0407 1945.10753 1945.10764 -0.06 -4e-05 956196.1875 CID (Collision Induced Dissociation) MS2 3.011489 20 67.0 60.0 117.0145 39045 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 18.9 53.4 105.3 19.5 61.9 124.6 26.7 61.5 128.6 69.2 Excluded by Method 3.65 1 0.0004462 0.00149 False High Sequest HT (A2) Unambiguous ELSPAALEK [K].eLsPAALEk.[R] N-Term(TMT6plex); S3(Phospho); K9(TMT6plex) 1 O75683 O75683 0 2 0.2285 0 1 1 748.41246 1495.81765 1495.81733 0.21 0.00016 8552132.0 CID (Collision Induced Dissociation) MS2 25.05025 30 152.0 25.269 90.4014 29073 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 157.6 145.9 134.5 155.0 153.1 148.8 179.2 157.0 139.1 149.3 Excluded by Method 3.37 1 0.0004462 0.001686 False High Sequest HT (A2) Unambiguous LTRGLCIERLSASPAQEK [K].lTRGLcIERLSAsPAQEk.[E] N-Term(TMT6plex); C6(Carbamidomethyl); S13(Phospho); K18(TMT6plex) 1 Q9Y2F5 Q9Y2F5 2 4 0.1254 0 1 1 642.59887 2567.37365 2567.37808 -1.72 -0.00111 440586.0625 CID (Collision Induced Dissociation) MS2 17.66329 20 74.6 60.0 87.4517 28044 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 83.4 84.8 62.5 64.7 72.8 77.4 83.3 75.4 71.1 70.6 Excluded by Method 3.03 1 0.0004462 0.001041 False High Sequest HT (A2) Unambiguous DSISAVSSEKVSPSK [K].dSISAVSSEkVsPSk.[S] N-Term(TMT6plex); K10(TMT6plex); S12(Phospho); K15(TMT6plex) 1 P46821 P46821 1 3 0.1108 0 1 1 763.41692 2288.23621 2288.23537 0.37 0.00028 846474.125 CID (Collision Induced Dissociation) MS2 8.553244 10 102.1 60.0 73.1425 22667 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 106.5 97.4 77.6 121.4 90.8 96.7 124.0 99.9 98.9 107.4 Excluded by Method 3.25 1 0.0004462 0.0006157 False High Sequest HT (A2) Unambiguous EEPLSEEEPCTSTAIASPEK [K].eEPLsEEEPcTSTAIASPEk.[K] N-Term(TMT6plex); S5(Phospho); C10(Carbamidomethyl); K20(TMT6plex) 1 Q9Y2X3 Q9Y2X3 0 4 0.2289 0 1 1 686.32569 2742.28094 2742.28329 -0.86 -0.00059 3816656.5 CID (Collision Induced Dissociation) MS2 11.86847 30 120.6 17.664 87.5608 28085 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 123.8 113.8 100.6 115.8 111.6 114.3 136.8 142.8 131.6 115.1 Excluded by Method 5.46 1 0.0004462 0.0007471 False High Sequest HT (A2) Unambiguous RESVASGDDRAEEDMDEAIEK [K].rEsVASGDDRAEEDMDEAIEk.[G] N-Term(TMT6plex); S3(Phospho); K21(TMT6plex) 1 P46821 P46821 2 4 0.003 0 1 1 723.33499 2890.31814 2890.31778 0.12 9e-05 991712.75 CID (Collision Induced Dissociation) MS2 3.175992 20 46.2 60.0 77.6513 24366 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 49.5 52.6 39.3 41.7 40.6 46.0 53.7 47.4 39.6 51.6 Excluded by Method 3.38 1 0.0004462 0.0007192 False High Sequest HT (A2) Unambiguous VQGEAVSNIQENTQTPTVQEESEEEEVDETGVEVK [K].vQGEAVSNIQENTQTPTVQEEsEEEEVDETGVEVk.[D] N-Term(TMT6plex); S22(Phospho); K35(TMT6plex) 3 Q13765 Q13765; E9PAV3; E9PAV3-2 0 4 0.0285 0 1 1 1100.51342 4399.03184 4399.06016 -6.44 -0.00708 554685.125 CID (Collision Induced Dissociation) MS2 0.0 60 13.8 60.0 117.9813 39403 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 14.5 12.0 10.9 15.1 16.0 15.1 11.8 15.6 14.8 11.7 Excluded by Method 4.92 1 0.0004462 0.0007851 False High Sequest HT (A2) Unambiguous IELSDDSDVEVHPNVDKK [K].iELsDDsDVEVHPNVDkk.[S] N-Term(TMT6plex); S4(Phospho); S7(Phospho); K17(TMT6plex); K18(TMT6plex) 1 P06101 P06101 1 3 0.5763 0 1 1 962.80966 2886.41441 2886.41421 0.07 7e-05 677142.5 CID (Collision Induced Dissociation) MS2 23.89934 20 82.2 60.0 90.7734 29212 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 23.4 64.8 116.8 30.5 71.6 147.3 27.2 81.7 168.5 90.2 Excluded by Method 3.8 1 0.0004462 0.000901 False High Sequest HT (A2) Unambiguous VVGLSTLPEIYEK [K].vVGLSTLPEIYEk.[M] N-Term(TMT6plex); K13(TMT6plex) 1 P00330 P00330 0 3 0.4114 0 1 1 636.04853 1906.13104 1906.13014 0.47 0.0003 686004.375 CID (Collision Induced Dissociation) MS2 4.743675 40 75.1 60.0 122.6686 41098 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 20.3 59.8 112.3 25.2 56.0 140.0 29.1 76.7 146.0 85.2 Excluded by Method 2.99 1 0.0004462 0.001252 False High Sequest HT (A2) Unambiguous EEEAKEESDDDMGFGLFD [K].eEEAkEEsDDDmGFGLFD.[-] N-Term(TMT6plex); K5(TMT6plex); S8(Phospho); M12(Oxidation) 1 P02400 P02400 1 3 0.6813 0 1 1 873.03699 2617.09642 2617.0941 0.89 0.00077 1878083.75 CID (Collision Induced Dissociation) MS2 13.91338 30 123.3 60.0 119.4333 39905 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 31.4 103.2 187.2 42.1 93.5 240.4 44.5 111.2 247.5 131.8 Excluded by Method 3.42 1 0.0004462 0.001153 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVssDEEF.[-] N-Term(TMT6plex); S8(Phospho); S9(Phospho) 1 P16521 P16521 0 2 0.1299 0 1 1 925.35405 1849.70081 1849.70195 -0.62 -0.00057 1754399.625 CID (Collision Induced Dissociation) MS2 21.47517 50 102.9 60.0 114.6853 38193 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 27.5 81.1 164.1 28.4 86.7 196.6 43.8 85.0 210.6 105.1 Excluded by Method 4.31 1 0.0004462 0.0006494 False High Sequest HT (A2) Unambiguous EEEAAEESDDDMGFGLFD [K].eEEAAEESDDDmGFGLFD.[-] N-Term(TMT6plex); M12(Oxidation) 2 P05319 P10622; P05319 0 3 0.7047 0 1 1 750.97342 2250.90571 2250.90698 -0.57 -0.00043 635606.375 CID (Collision Induced Dissociation) MS2 0.0 40 49.0 60.0 114.1068 37980 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 15.5 37.0 73.2 15.9 40.1 103.1 18.3 44.0 94.1 49.1 Excluded by Method 3.42 1 0.0004462 0.0007774 False High Sequest HT (A2) Unambiguous EEEEAKEESDDDMGFGLFD [K].eEEEAkEEsDDDmGFGLFD.[-] N-Term(TMT6plex); K6(TMT6plex); S9(Phospho); M13(Oxidation) 1 P05318 P05318 1 3 0.6658 0 1 1 916.05071 2746.13759 2746.13669 0.33 0.0003 3551367.75 CID (Collision Induced Dissociation) MS2 3.500781 40 129.3 26.034 114.0606 37962 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 29.8 110.3 183.7 43.6 105.8 257.4 42.5 118.9 271.3 130.0 Excluded by Method 3.68 1 0.0004462 0.0008253 False High Sequest HT (A2) Unambiguous AAADALSDLEIK [K].aAADALsDLEIk.[D] N-Term(TMT6plex); S7(Phospho); K12(TMT6plex) 1 P09938 P09938 0 3 0.0831 0 1 1 585.64997 1754.93536 1754.93415 0.69 0.0004 497928.46875 CID (Collision Induced Dissociation) MS2 8.137167 40 59.0 60.0 113.9113 37910 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 16.7 50.0 86.0 23.5 48.7 108.7 25.0 51.1 124.2 55.9 Excluded by Method 3.13 1 0.0004462 0.0009208 False High Sequest HT (A2) Unambiguous RLGSLVDEFK [K].rLGsLVDEFk.[E] N-Term(TMT6plex); S4(Phospho); K10(TMT6plex) 2 P12956 P12956; P12956-2 1 3 0.4737 0 1 1 567.983 1701.93446 1701.93409 0.21 0.00012 1133038.25 CID (Collision Induced Dissociation) MS2 7.127844 80 113.3 60.0 115.0833 38340 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 113.4 114.0 93.4 117.8 109.3 119.7 121.1 131.0 107.0 105.9 3.42 1 0.0004462 0.001932 False High Sequest HT (A2) Unambiguous SSVSPTAGSFGLSK [K].sSVsPTAGSFGLSk.[D] N-Term(TMT6plex); S4(Phospho); K14(TMT6plex) 1 Q07084 Q07084 0 3 0.0137 0 1 1 621.66049 1862.96693 1862.96652 0.22 0.00014 1838044.625 CID (Collision Induced Dissociation) MS2 21.92695 40 63.8 55.494 91.9749 29666 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 16.2 53.6 98.5 19.8 48.7 121.8 27.7 65.0 118.8 67.4 Excluded by Method 3.65 1 0.0004462 0.0009206 False High Sequest HT (A2) Unambiguous VQGEAVSNIQENTQTPTVQEESEEEEVDETGVEVK [K].vQGEAVSNIQENTQTPTVQEEsEEEEVDETGVEVk.[D] N-Term(TMT6plex); S22(Phospho); K35(TMT6plex) 3 Q13765 Q13765; E9PAV3; E9PAV3-2 0 4 0.0282 0 1 1 1100.51781 4399.04942 4399.06016 -2.44 -0.00269 310770.375 CID (Collision Induced Dissociation) MS2 0.0 40 15.7 60.0 115.9005 38633 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 18.2 13.6 12.4 16.8 14.4 18.0 16.8 16.0 16.7 14.4 Excluded by Method 4.25 1 0.0004462 0.0005985 False High Sequest HT (A2) Unambiguous IEVLDSPASK [K].iEVLDsPASk.[K] N-Term(TMT6plex); S6(Phospho); K10(TMT6plex) 1 Q8N5I9 Q8N5I9 0 2 0.1858 0 1 1 798.93636 1596.86544 1596.86501 0.27 0.00021 1815273.75 CID (Collision Induced Dissociation) MS2 0.0 40 165.3 60.0 92.0501 29695 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 178.2 165.2 139.4 180.7 148.3 152.5 197.9 171.4 162.0 157.7 Excluded by Method 3.93 1 0.0004462 0.001908 False High Sequest HT (A2) Unambiguous NDFEIVLSEDEK [K].nDFEIVLsEDEk.[E] N-Term(TMT6plex); S8(Phospho); K12(TMT6plex) 1 Q03654 Q03654 0 3 0.3298 0 1 1 659.32693 1975.96624 1975.96658 -0.17 -0.00011 922187.9375 CID (Collision Induced Dissociation) MS2 0.0 70 41.8 60.0 112.4704 37339 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 20.6 40.3 57.7 20.4 32.9 67.3 24.2 37.3 74.4 42.5 3.79 1 0.0004462 0.0006488 False High Sequest HT (A2) Unambiguous DDQLEYLEEK [K].dDQLEYLEEk.[R] N-Term(TMT6plex); K10(TMT6plex) 2 P15108 P02829; P15108 0 3 0.371 0 1 1 580.64183 1739.91094 1739.91037 0.33 0.00019 848073.9375 CID (Collision Induced Dissociation) MS2 0.0 60 177.1 60.0 92.1082 29716 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 44.8 148.6 269.8 59.6 150.3 332.2 64.4 159.8 352.2 188.8 Excluded by Method 3.1 1 0.0004462 0.0005973 False High Sequest HT (A2) Unambiguous DVTPPPETEVVLIK [K].dVtPPPETEVVLIk.[N] N-Term(TMT6plex); T3(Phospho); K14(TMT6plex) 3 P27816 P27816; P27816-6; P27816-2 0 3 0.2175 0 1 1 692.38604 2075.14355 2075.14415 -0.29 -0.0002 1528433.875 CID (Collision Induced Dissociation) MS2 7.146087 40 87.4 60.0 115.5638 38514 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 93.4 79.2 74.9 93.0 89.7 83.2 102.6 86.5 88.8 82.3 Excluded by Method 3.54 1 0.0004462 0.0006049 False High Sequest HT (A2) Unambiguous SSLESPTMLK [K].sSLEsPTMLk.[L] N-Term(TMT6plex); S5(Phospho); K10(TMT6plex) 1 Q12044 Q12044 0 2 0.0648 0 1 1 815.93017 1630.85306 1630.85273 0.2 0.00017 1443582.5 CID (Collision Induced Dissociation) MS2 0.0 20 77.1 60.0 88.9757 28620 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 24.1 62.9 114.0 29.4 68.8 130.3 40.5 68.7 152.2 79.8 Excluded by Method 3.55 1 0.0004462 0.0009418 False High Sequest HT (A2) Unambiguous SKPIPIMPASPQK [K].skPIPIMPAsPQk.[G] N-Term(TMT6plex); K2(TMT6plex); S10(Phospho); K13(TMT6plex) 9 O00429 O00429-4; O00429-9; O00429; O00429-6; O00429-8; O00429-7; O00429-3; O00429-5; O00429-2 0 3 0.5437 0 1 1 721.08579 2161.24282 2161.24231 0.23 0.00017 3089467.0 CID (Collision Induced Dissociation) MS2 10.71597 20 122.9 30.908 90.2803 29027 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 122.1 126.1 84.8 129.8 117.2 119.3 144.3 121.0 125.3 138.9 Excluded by Method 5.04 1 0.0004462 0.0009643 False High Sequest HT (A2) Unambiguous KPTPLPSLK [K].kPtPLPsLk.[D] N-Term(TMT6plex); K1(TMT6plex); T3(Phospho); S7(Phospho); K9(TMT6plex) 1 P06105 P06105 0 3 0.5568 0 1 1 610.01653 1828.03505 1828.03535 -0.17 -0.0001 4705214.0 CID (Collision Induced Dissociation) MS2 7.564846 50 191.6 38.527 87.3301 27920 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 54.0 162.9 278.2 69.3 167.2 343.1 74.2 183.3 387.3 196.0 Excluded by Method 3.52 1 0.0004462 0.001602 False High Sequest HT (A2) Unambiguous EEPLSEEEPCTSTAIASPEK [K].eEPLSEEEPcTSTAIAsPEk.[K] N-Term(TMT6plex); C10(Carbamidomethyl); S17(Phospho); K20(TMT6plex) 1 Q9Y2X3 Q9Y2X3 0 4 0.1288 0 1 1 686.326 2742.28216 2742.28329 -0.41 -0.00028 1089587.0 CID (Collision Induced Dissociation) MS2 4.887118 40 30.8 60.0 85.155 27185 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 26.6 28.5 26.4 37.6 28.2 33.0 31.3 30.8 34.1 31.5 Excluded by Method 3.26 1 0.0004462 0.0006972 False High Sequest HT (A2) Unambiguous LFEESDDKEDEDADGK [K].lFEEsDDkEDEDADGk.[E] N-Term(TMT6plex); S5(Phospho); K8(TMT6plex); K16(TMT6plex) 1 O43719 O43719 1 3 0.398 0 1 1 870.40832 2609.21042 2609.21106 -0.25 -0.00022 726889.0625 CID (Collision Induced Dissociation) MS2 20.87454 40 243.2 60.0 85.6897 27388 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 229.4 250.4 159.4 321.0 234.5 206.3 302.8 253.3 252.2 223.1 Excluded by Method 3.04 1 0.0004462 0.0007153 False High Sequest HT (A2) Unambiguous SPSHDNEAGSVDNSEISQLLQSAATK [K].sPsHDNEAGSVDNSEISQLLQSAATk.[A] N-Term(TMT6plex); S3(Phospho); K26(TMT6plex) 1 P21538 P21538 0 3 0.0228 0 1 1 1075.18826 3223.55022 3223.55202 -0.56 -0.0006 393749.84375 CID (Collision Induced Dissociation) MS2 0.0 80 5.6 60.0 122.4507 41052 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 2.5 6.5 7.6 1.7 2.7 10.3 1.3 6.1 10.0 7.1 2.19 1 0.0004462 0.001094 False High Sequest HT (A2) Unambiguous RTCPSLSPTSPLNNK [K].rTcPSLsPTSPLNNk.[G] N-Term(TMT6plex); C3(Carbamidomethyl); S7(Phospho); K15(TMT6plex) 2 O75925 O75925; O75925-2 1 3 0.1 0 1 1 737.38508 2210.14068 2210.14047 0.09 7e-05 680169.0 CID (Collision Induced Dissociation) MS2 24.64407 40 66.3 60.0 73.4729 22701 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 55.8 60.7 65.1 61.8 54.6 79.7 63.3 70.4 85.3 66.0 Excluded by Method 4.4 1 0.0004462 0.0006246 False High Sequest HT (A2) Unambiguous DINAYNCEEPTEK [K].dINAYNcEEPTEk.[L] N-Term(TMT6plex); C7(Carbamidomethyl); K13(TMT6plex) 1 P30041 P30041 0 3 0.4514 0 1 1 681.00328 2040.99529 2040.99484 0.22 0.00015 838262.6875 CID (Collision Induced Dissociation) MS2 5.204955 60 25.6 60.0 66.8867 20243 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 23.1 21.4 20.1 24.7 27.5 23.3 27.8 30.8 33.0 24.2 Excluded by Method 2.88 1 0.0004462 0.001502 False High Sequest HT (A2) Unambiguous EMLASDDEEDVSSK [K].eMLAsDDEEDVSSk.[V] N-Term(TMT6plex); S5(Phospho); K14(TMT6plex) 4 Q0ZGT2 Q0ZGT2-2; Q0ZGT2-4; Q0ZGT2; Q0ZGT2-3 0 3 0.6907 0 1 1 698.31799 2092.93942 2092.93977 -0.17 -0.00012 4677041.0 CID (Collision Induced Dissociation) MS2 0.762118 40 67.2 27.859 78.2534 24591 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 64.5 66.6 49.3 67.2 68.9 65.0 80.5 75.4 59.9 75.0 Excluded by Method 3.33 1 0.0004462 0.001547 False High Sequest HT (A2) Unambiguous QSSLAEPVSPSK [K].qSSLAEPVsPSk.[K] N-Term(TMT6plex); S9(Phospho); K12(TMT6plex) 1 O43572 O43572 0 3 0.1111 0 1 1 589.98131 1767.92938 1767.9294 -0.01 -1e-05 1593522.25 CID (Collision Induced Dissociation) MS2 3.111281 50 137.9 60.0 66.5792 20187 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 135.4 134.3 109.5 149.9 126.2 128.7 153.0 148.7 146.2 147.5 Excluded by Method 3.51 1 0.0004462 0.0008409 False High Sequest HT (A2) Unambiguous FSVSPVVQVAVEVK [K].fSVSPVVQVAVEVk.[N] N-Term(TMT6plex); K14(TMT6plex) 1 P32324 P32324 0 3 0.4266 0 1 1 649.3958 1946.17284 1946.17267 0.09 6e-05 644970.1875 CID (Collision Induced Dissociation) MS2 54.45144 40 46.5 60.0 125.9012 42315 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 20.4 42.6 66.0 17.8 39.3 86.3 18.3 41.4 89.7 43.0 Excluded by Method 2.86 1 0.0004462 0.001036 False High Sequest HT (A2) Unambiguous SVESTSPEPSK [K].sVESTsPEPSk.[I] N-Term(TMT6plex); S6(Phospho); K11(TMT6plex) 10 P18583 P18583; P18583-4; P18583-6; P18583-5; P18583-10; P18583-9; P18583-2; P18583-3; P18583-7; P18583-8 0 3 0.0938 0 1 1 562.61818 1685.83999 1685.83992 0.04 2e-05 665353.4375 CID (Collision Induced Dissociation) MS2 21.71887 40 89.9 60.0 50.5683 14166 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 80.5 89.6 76.9 91.2 79.6 91.4 96.6 97.4 97.8 97.9 Excluded by Method 3.2 1 0.0004462 0.001013 False High Sequest HT (A2) Unambiguous SVGFGLVYNSVAEAK [K].sVGFGLVYNSVAEAk.[K] N-Term(TMT6plex); K15(TMT6plex) 1 P0CX31 P0CX31 0 3 0.3697 0 1 1 667.04689 1999.12611 1999.12645 -0.17 -0.00011 269474.46875 CID (Collision Induced Dissociation) MS2 38.16425 40 17.6 60.0 118.6011 39598 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 9.2 17.9 22.4 8.0 16.7 30.6 7.9 15.0 29.4 18.9 Excluded by Method 2.11 1 0.0004462 0.0008053 False High Sequest HT (A2) Unambiguous AEDDTGEEEEDDPVIEEFPLK [K].aEDDtGEEEEDDPVIEEFPLk.[I] N-Term(TMT6plex); T5(Phospho); K21(TMT6plex) 1 P36121 P36121 0 3 0.5457 0 1 1 982.11251 2944.32299 2944.32766 -1.59 -0.00156 1073257.25 CID (Collision Induced Dissociation) MS2 14.17285 70 19.7 60.0 125.6173 42211 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 15.0 21.3 21.1 13.1 18.2 25.0 10.3 23.2 27.1 23.0 3.83 1 0.0004462 0.002027 False High Sequest HT (A2) Unambiguous VLDEEGSEREFDEDSDEK [K].vLDEEGsEREFDEDsDEk.[E] N-Term(TMT6plex); S7(Phospho); S15(Phospho); K18(TMT6plex) 1 O43719 O43719 1 3 0.624 0 1 1 916.05272 2746.14361 2746.14218 0.52 0.00048 1106221.75 CID (Collision Induced Dissociation) MS2 0.0 30 93.9 60.0 86.4441 27587 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 88.1 94.2 75.1 110.1 101.4 82.0 106.8 95.3 90.9 94.9 Excluded by Method 5.0 1 0.0004462 0.0013 False High Sequest HT (A2) Unambiguous LSSETPALESEGPQTK [K].lSSEtPALESEGPQTk.[K] N-Term(TMT6plex); T5(Phospho); K16(TMT6plex) 1 P38205 P38205 0 3 0.0405 0 1 1 738.04291 2212.11419 2212.11503 -0.38 -0.00028 2162301.75 CID (Collision Induced Dissociation) MS2 47.95189 50 215.8 58.316 86.9775 27789 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 231.1 226.4 174.9 229.9 213.5 198.8 242.1 228.5 219.4 193.6 Excluded by Method 2.96 1 0.0004462 0.0006509 False High Sequest HT (A2) Unambiguous TVLSDSAHFDVK [K].tVLSDsAHFDVk.[H] N-Term(TMT6plex); S6(Phospho); K12(TMT6plex) 1 P07260 P07260 0 3 0.145 0 1 1 619.65726 1856.95724 1856.95595 0.69 0.00043 828764.625 CID (Collision Induced Dissociation) MS2 30.12467 40 81.3 60.0 88.3537 28307 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 21.6 69.6 123.7 28.4 60.2 153.3 26.5 76.7 158.0 94.7 Excluded by Method 3.31 1 0.0004462 0.002144 False High Sequest HT (A2) Unambiguous SPSLSPSPPSPLEK [K].sPSLSPSPPsPLEk.[T] N-Term(TMT6plex); S10(Phospho); K14(TMT6plex) 1 P46821 P46821 0 3 0.4521 0 1 1 654.3527 1961.04353 1961.03968 1.97 0.00128 638346.625 CID (Collision Induced Dissociation) MS2 16.35151 60 224.6 60.0 89.2238 28632 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 237.8 229.2 193.3 236.6 214.7 209.2 254.2 221.6 222.7 226.4 Excluded by Method 3.76 1 0.0004462 0.001227 False High Sequest HT (A2) Unambiguous SSLESPTMLK [K].sSLEsPTMLk.[L] N-Term(TMT6plex); S5(Phospho); K10(TMT6plex) 1 Q12044 Q12044 0 2 0.0632 0 1 1 815.92967 1630.85207 1630.85273 -0.41 -0.00033 2529622.5 CID (Collision Induced Dissociation) MS2 17.06982 30 81.3 60.0 89.279 28652 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 24.9 62.7 114.3 23.9 70.7 169.7 22.7 84.9 149.7 89.8 Excluded by Method 3.64 1 0.0004462 0.001547 False High Sequest HT (A2) Unambiguous DQDQSSPKVEVTSEDEK [K].dQDQSsPkVEVTsEDEk.[E] N-Term(TMT6plex); S6(Phospho); K8(TMT6plex); S13(Phospho); K17(TMT6plex) 1 P07264 P07264 1 3 0.2197 0 1 1 923.43482 2768.2899 2768.28834 0.56 0.00052 584186.875 CID (Collision Induced Dissociation) MS2 57.24821 30 221.2 60.0 65.8387 19910 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 66.8 195.1 287.9 93.9 198.7 375.7 98.7 197.0 464.7 233.1 Excluded by Method 5.28 1 0.0004462 0.001577 False High Sequest HT (A2) Unambiguous RSRLTPVSPESSSTEEK [K].rsRLTPVSPESSSTEEk.[S] N-Term(TMT6plex); S2(Phospho); K17(TMT6plex) 2 Q13501 Q13501-2; Q13501 2 4 0.07 0 1 1 607.81775 2428.24916 2428.2485 0.27 0.00016 2962179.5 CID (Collision Induced Dissociation) MS2 3.944857 20 90.9 29.433 53.0221 15076 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 89.7 98.5 76.1 93.9 86.5 89.6 98.4 99.5 83.8 93.2 Excluded by Method 3.0 1 0.0004462 0.0009481 False High Sequest HT (A2) Unambiguous EEEPILAPSVK [K].eEEPILAPsVk.[D] N-Term(TMT6plex); S9(Phospho); K11(TMT6plex) 1 P05750 P05750 0 3 0.461 0 1 1 583.98619 1749.944 1749.94399 0.01 0.0 3503182.75 CID (Collision Induced Dissociation) MS2 13.09132 60 128.4 21.46 90.1756 28986 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 27.6 107.4 205.3 43.6 105.2 243.4 43.5 120.6 261.0 126.0 Excluded by Method 2.95 1 0.0004462 0.002056 False High Sequest HT (A2) Unambiguous EEEAKEESDDDMGFGLFD [K].eEEAkEEsDDDmGFGLFD.[-] N-Term(TMT6plex); K5(TMT6plex); S8(Phospho); M12(Oxidation) 1 P02400 P02400 1 3 0.5957 0 1 1 873.03545 2617.0918 2617.0941 -0.88 -0.00077 1565154.0 CID (Collision Induced Dissociation) MS2 4.276393 40 92.7 48.32 119.3773 39925 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 20.4 75.0 136.1 32.2 74.8 175.5 34.3 85.7 200.3 92.6 Excluded by Method 3.71 1 0.0004462 0.001939 False High Sequest HT (A2) Unambiguous GLSPLEEEK [K].gLsPLEEEk.[L] N-Term(TMT6plex); S3(Phospho); K9(TMT6plex) 1 P31374 P31374 0 3 0.4435 0 1 1 513.94086 1539.80804 1539.80716 0.57 0.00029 862459.0 CID (Collision Induced Dissociation) MS2 0.0 40 40.8 60.0 87.152 27933 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 15.9 32.9 56.2 15.8 35.8 70.1 17.7 41.7 80.3 41.6 Excluded by Method 3.54 1 0.0004462 0.001878 False High Sequest HT (A2) Unambiguous EAPAEGEAAEPGSPTAAEGEAASAASSTSSPK [K].eAPAEGEAAEPGsPTAAEGEAASAASSTSSPk.[A] N-Term(TMT6plex); S13(Phospho); K32(TMT6plex) 1 P29966 P29966 0 4 0.0263 0 1 1 864.15304 3453.59031 3453.59467 -1.26 -0.00109 454652.75 CID (Collision Induced Dissociation) MS2 0.0 60 14.1 60.0 73.4297 22683 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 18.2 14.8 14.8 16.3 12.8 12.9 13.4 10.6 13.9 13.1 Excluded by Method 2.66 1 0.0004462 0.002064 False High Sequest HT (A2) Unambiguous EEEAKEESDDDMGFGLFD [K].eEEAkEEsDDDmGFGLFD.[-] N-Term(TMT6plex); K5(TMT6plex); S8(Phospho); M12(Oxidation) 1 P02400 P02400 1 3 0.6601 0 1 1 873.03637 2617.09455 2617.0941 0.17 0.00015 1429754.25 CID (Collision Induced Dissociation) MS2 0.0 40 185.1 60.0 120.5002 40338 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 44.1 152.2 264.5 65.9 151.8 337.4 64.4 170.7 395.3 204.3 Excluded by Method 3.06 1 0.0004462 0.000758 False High Sequest HT (A2) Unambiguous NFHAEVSTPQVLSAK [K].nFHAEVStPQVLSAk.[K] N-Term(TMT6plex); T8(Phospho); K15(TMT6plex) 1 P48570 P48570 0 3 0.0758 0 1 1 722.71768 2166.1385 2166.13604 1.14 0.00082 552241.0 CID (Collision Induced Dissociation) MS2 8.918163 80 72.2 60.0 88.3809 28317 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 22.4 56.9 109.6 28.6 54.4 134.0 39.3 64.4 130.6 81.3 3.56 1 0.0004462 0.0008761 False High Sequest HT (A2) Unambiguous LTNRLDDLLERSQELTISK [K].lTNRLDDLLERSQELtISk.[E] N-Term(TMT6plex); T16(Phospho); K19(TMT6plex) 1 P38853 P38853 2 4 0.0638 0 1 1 696.38395 2782.51399 2782.5116 0.86 0.0006 413568.6875 CID (Collision Induced Dissociation) MS2 14.65532 30 72.4 60.0 122.0765 40912 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 20.3 65.1 102.8 24.6 57.9 135.6 24.5 64.5 151.7 76.8 Excluded by Method 3.92 1 0.0004462 0.00196 False High Sequest HT (A2) Unambiguous TVDSQGPTPVCTPTFLERRK [K].tVDSQGPtPVctPTFLERRk.[S] N-Term(TMT6plex); T8(Phospho); C11(Carbamidomethyl); T12(Phospho); K20(TMT6plex) 1 Q9BYG3 Q9BYG3 2 4 0.3659 0 1 1 727.61099 2907.42212 2907.42412 -0.69 -0.0005 811031.3125 CID (Collision Induced Dissociation) MS2 0.0 40 70.6 60.0 86.2898 27614 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 68.6 68.5 59.7 73.5 64.2 75.4 82.7 73.7 70.4 69.6 Excluded by Method 4.4 1 0.0004462 0.001432 False High Sequest HT (A2) Unambiguous ETQSPEQVK [K].eTQsPEQVk.[S] N-Term(TMT6plex); S4(Phospho); K9(TMT6plex) 3 Q9NYF8 Q9NYF8-3; Q9NYF8; Q9NYF8-2 0 3 0.0524 0 1 1 528.60763 1583.80833 1583.80822 0.07 4e-05 3656767.25 CID (Collision Induced Dissociation) MS2 5.036543 60 184.0 54.191 47.2762 12997 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 197.4 188.3 162.9 179.1 167.5 181.1 205.3 185.1 178.0 195.5 Excluded by Method 2.86 1 0.0004462 0.001281 False High Sequest HT (A2) Unambiguous ATVNARPQRVLDTSSLTQSAPASPTNK [K].aTVNARPQRVLDTSSLTQsAPAsPTNk.[G] N-Term(TMT6plex); S19(Phospho); S23(Phospho); K27(TMT6plex) 2 Q8N122 Q8N122-3; Q8N122 1 4 0.2586 0 1 1 857.93922 3428.73505 3428.73403 0.3 0.00025 2826334.25 CID (Collision Induced Dissociation) MS2 0.0 40 33.1 40.392 77.7394 24345 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 37.5 28.8 26.7 35.4 27.4 39.7 33.1 32.7 38.5 31.1 Excluded by Method 3.48 1 0.0004462 0.001035 False High Sequest HT (A2) Unambiguous GRAEGEWEDQEALDYFSDK [K].gRAEGEWEDQEALDYFsDk.[E] N-Term(TMT6plex); S17(Phospho); K19(TMT6plex) 3 Q9NYF8 Q9NYF8-3; Q9NYF8; Q9NYF8-2 1 3 0.1292 0 1 1 928.42413 2783.25784 2783.26019 -0.84 -0.00078 481877.03125 CID (Collision Induced Dissociation) MS2 11.48146 40 34.0 60.0 107.6205 35600 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 30.5 36.0 33.6 31.9 29.2 35.2 36.4 33.9 38.5 34.4 Excluded by Method 3.56 1 0.0004462 0.0008625 False High Sequest HT (A2) Unambiguous ALVLELDEMSNGSEIQDALEEISGQK [K].aLVLELDEmsNGSEIQDALEEISGQk.[T] N-Term(TMT6plex); M9(Oxidation); S10(Phospho); K26(TMT6plex) 2 P17695 P17695; P17695-2 0 4 0.0739 0 1 1 843.92212 3372.66663 3372.65337 3.93 0.00332 1257387.625 CID (Collision Induced Dissociation) MS2 15.81632 50 55.9 60.0 133.8417 45626 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 17.9 43.7 80.9 23.5 44.9 95.9 29.1 56.5 108.2 58.0 Excluded by Method 5.01 1 0.0004462 0.00085 False High Sequest HT (A2) Unambiguous VPSPLEGSEGDGDTD [K].vPSPLEGSEGDGDtD.[-] N-Term(TMT6plex); T14(Phospho) 2 Q9Y606 Q9Y606; Q9Y606-2 0 3 0.1242 0 1 1 595.25439 1783.74862 1783.74725 0.77 0.00046 183373.28125 CID (Collision Induced Dissociation) MS2 14.53721 20 47.6 60.0 87.9086 28157 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 45.2 43.8 52.5 33.8 50.7 47.3 55.6 50.5 41.5 55.2 Excluded by Method 3.22 1 0.0004462 0.001195 False High Sequest HT (A2) Unambiguous ESSSEDLLK [K].eSsSEDLLk.[R] N-Term(TMT6plex); S3(Phospho); K9(TMT6plex) 1 P47048 P47048 0 2 0.108 0 1 1 773.3941 1545.78093 1545.78134 -0.26 -0.0002 3842667.5 CID (Collision Induced Dissociation) MS2 2.368178 50 151.2 45.239 77.0552 24091 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 40.5 116.0 216.2 49.4 135.1 284.1 47.0 135.9 315.3 172.5 Excluded by Method 3.61 1 0.0004462 0.001025 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVSsDEEF.[-] N-Term(TMT6plex); S9(Phospho) 1 P16521 P16521 0 2 0.0477 0 1 1 885.37132 1769.73536 1769.73562 -0.15 -0.00013 1151830.625 CID (Collision Induced Dissociation) MS2 20.51882 50 113.8 48.55 119.6205 40064 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 29.6 81.2 198.6 29.8 86.3 222.1 44.1 108.3 210.3 127.2 Excluded by Method 3.77 1 0.0004462 0.0007061 False High Sequest HT (A2) Unambiguous DKGSDDDDDDEEFDK [K].dkGsDDDDDDEEFDk.[I] N-Term(TMT6plex); K2(TMT6plex); S4(Phospho); K15(TMT6plex) 1 Q05468 Q05468 1 3 0.4444 0 1 1 838.03617 2512.09395 2512.08553 3.35 0.00281 958748.3125 CID (Collision Induced Dissociation) MS2 33.952 20 24.8 60.0 64.7945 19484 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 16.5 22.6 25.1 21.6 21.5 34.3 25.2 20.4 35.3 25.8 Excluded by Method 4.23 1 0.0004462 0.001259 False High Sequest HT (A2) Unambiguous IEEVLSPEGSPSK [K].iEEVLsPEGsPSk.[S] N-Term(TMT6plex); S6(Phospho); S10(Phospho); K13(TMT6plex) 2 Q9UEY8 Q9UEY8-2; Q9UEY8 0 3 0.3729 0 1 1 663.9913 1989.95936 1989.95873 0.32 0.00021 949575.625 CID (Collision Induced Dissociation) MS2 16.3013 50 51.2 60.0 103.4071 34037 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 56.1 43.6 40.3 53.5 41.3 57.9 57.6 49.7 61.0 51.4 Excluded by Method 3.54 1 0.0004462 0.001881 False High Sequest HT (A2) Unambiguous SPEEEGAVSS [K].sPEEEGAVSs.[-] N-Term(TMT6plex); S10(Phospho) 2 Q01130 Q01130-2; Q01130 0 2 0.0199 0 1 1 650.77928 1300.55128 1300.55072 0.43 0.00028 2833108.25 CID (Collision Induced Dissociation) MS2 3.71175 80 181.2 60.0 40.5607 10573 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 178.9 171.2 152.8 179.4 171.7 187.8 194.4 204.2 182.5 189.0 3.01 1 0.0004462 0.001771 False High Sequest HT (A2) Unambiguous LTRGLCIERLSASPAQEK [K].lTRGLcIERLSAsPAQEk.[E] N-Term(TMT6plex); C6(Carbamidomethyl); S13(Phospho); K18(TMT6plex) 1 Q9Y2F5 Q9Y2F5 2 4 0.0517 0 1 1 642.59966 2567.37683 2567.37808 -0.49 -0.00031 1947539.375 CID (Collision Induced Dissociation) MS2 6.432274 20 58.1 60.0 87.0152 27827 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 54.8 58.1 51.0 59.5 50.1 64.0 59.5 63.3 63.6 57.2 Excluded by Method 3.48 1 0.0004462 0.001117 False High Sequest HT (A2) Unambiguous AAADALSDLEIK [K].aAADALsDLEIk.[D] N-Term(TMT6plex); S7(Phospho); K12(TMT6plex) 1 P09938 P09938 0 3 0.1765 0 1 1 585.6491 1754.93275 1754.93415 -0.8 -0.00047 450492.5 CID (Collision Induced Dissociation) MS2 51.75706 40 270.9 60.0 113.2283 37692 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 67.6 215.9 419.9 91.6 220.3 515.9 108.1 247.9 565.5 255.9 Excluded by Method 3.23 1 0.0004462 0.0005756 False High Sequest HT (A2) Unambiguous ALVLELDEMSNGSEIQDALEEISGQK [K].aLVLELDEmsNGSEIQDALEEISGQk.[T] N-Term(TMT6plex); M9(Oxidation); S10(Phospho); K26(TMT6plex) 2 P17695 P17695; P17695-2 0 4 0.0886 0 1 1 843.91897 3372.65405 3372.65337 0.2 0.00017 463541.125 CID (Collision Induced Dissociation) MS2 31.77326 50 27.8 60.0 141.3507 48228 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 15.0 29.4 38.9 11.7 23.6 41.9 12.5 25.6 52.4 26.9 Excluded by Method 3.61 1 0.0004462 0.0008312 False High Sequest HT (A2) Unambiguous DVQSPGFLNEPLSSK [K].dVQsPGFLNEPLSSk.[S] N-Term(TMT6plex); S4(Phospho); K15(TMT6plex) 4 Q8NG31 Q8NG31-4; Q8NG31-3; Q8NG31-2; Q8NG31 0 3 0.2375 0 1 1 719.37308 2156.10469 2156.10407 0.29 0.00021 428909.25 CID (Collision Induced Dissociation) MS2 30.46784 40 46.1 60.0 107.2148 35448 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 46.1 45.4 29.9 44.6 49.0 52.5 49.5 48.9 49.3 46.1 Excluded by Method 4.21 1 0.0004462 0.0006159 False High Sequest HT (A2) Unambiguous EEEAAEESDDDMGFGLFD [K].eEEAAEEsDDDmGFGLFD.[-] N-Term(TMT6plex); S8(Phospho); M12(Oxidation) 2 P05319 P10622; P05319 0 2 0.7797 0 1 1 1165.94189 2330.87649 2330.87332 1.36 0.00159 9992497.0 CID (Collision Induced Dissociation) MS2 53.437 70 37.5 8.751 129.0798 43706 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 8.8 28.2 64.1 8.7 36.1 72.5 10.7 33.6 71.6 40.4 3.95 1 0.0004462 0.000646 False High Sequest HT (A2) Unambiguous SPAEVKSPEK [K].sPAEVksPEk.[A] N-Term(TMT6plex); K6(TMT6plex); S7(Phospho); K10(TMT6plex) 2 P12036 P12036; P12036-2 1 4 0.3361 0 1 1 460.51116 1839.02282 1839.02319 -0.2 -9e-05 666703.9375 CID (Collision Induced Dissociation) MS2 16.14244 60 39.4 60.0 55.4653 16013 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 32.9 38.0 31.9 49.5 32.6 39.1 44.1 44.8 42.5 38.4 Excluded by Method 2.38 1 0.0004462 0.001316 False High Sequest HT (A2) Unambiguous VEMYSGSDDDDDFNK [K].vEmYSGsDDDDDFNk.[L] N-Term(TMT6plex); M3(Oxidation); S7(Phospho); K15(TMT6plex) 1 O60841 O60841 0 3 0.1061 0 1 1 764.32094 2290.94826 2290.94631 0.85 0.00065 308527.09375 CID (Collision Induced Dissociation) MS2 6.952735 40 38.3 60.0 77.877 24397 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 33.8 35.9 31.5 34.9 34.0 40.9 39.4 44.2 44.9 43.2 Excluded by Method 3.11 1 0.0004462 0.0005687 False High Sequest HT (A2) Unambiguous ANTSGDFEK [K].aNTsGDFEk.[D] N-Term(TMT6plex); S4(Phospho); K9(TMT6plex) 7 P49792 P0DJD0; P49792; Q99666; P0DJD1; O14715; A6NKT7; Q7Z3J3 0 2 0.1176 0 1 1 753.86624 1506.7252 1506.72416 0.69 0.00052 5213559.0 CID (Collision Induced Dissociation) MS2 0.0 40 268.2 60.0 59.6584 17560 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 273.2 262.0 219.1 273.2 262.2 262.9 309.7 282.5 268.4 268.9 Excluded by Method 3.74 1 0.0004462 0.0008769 False High Sequest HT (A2) Unambiguous VFDDESDEK [K].vFDDEsDEk.[E] N-Term(TMT6plex); S6(Phospho); K9(TMT6plex) 1 O43719 O43719 0 2 0.2759 0 1 1 811.37318 1621.73908 1621.73987 -0.49 -0.00039 788605.125 CID (Collision Induced Dissociation) MS2 22.38145 40 74.0 30.282 75.6003 23537 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 67.1 68.7 61.8 76.0 67.9 72.9 80.8 70.3 99.1 75.2 Excluded by Method 3.77 1 0.0004462 0.001253 False High Sequest HT (A2) Unambiguous SELCSSPLGK [K].sELcSsPLGk.[R] N-Term(TMT6plex); C4(Carbamidomethyl); S6(Phospho); K10(TMT6plex) 1 Q9Y2F5 Q9Y2F5 0 3 0.0719 0 1 1 539.27656 1615.81513 1615.81668 -0.96 -0.00052 3134171.0 CID (Collision Induced Dissociation) MS2 39.52729 80 222.4 51.448 75.4232 23469 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 210.9 208.4 182.5 237.5 207.6 237.6 244.0 243.2 227.4 225.2 3.06 1 0.0004462 0.000775 False High Sequest HT (A2) Unambiguous VQGEAVSNIQENTQTPTVQEESEEEEVDETGVEVK [K].vQGEAVSNIQENTQTPTVQEEsEEEEVDETGVEVk.[D] N-Term(TMT6plex); S22(Phospho); K35(TMT6plex) 3 Q13765 Q13765; E9PAV3; E9PAV3-2 0 4 0.0689 0 1 1 1100.52279 4399.06931 4399.06016 2.08 0.00229 1074334.625 CID (Collision Induced Dissociation) MS2 0.0 10 10.2 31.568 130.574 44305 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 9.9 12.6 8.1 7.8 9.2 15.8 10.3 10.6 7.2 10.7 Excluded by Method 3.92 1 0.0004462 0.001887 False High Sequest HT (A2) Unambiguous EMEVEESPEK [K].eMEVEEsPEk.[I] N-Term(TMT6plex); S7(Phospho); K10(TMT6plex) 2 Q8N3X1 Q8N3X1-2; Q8N3X1 0 3 0.437 0 1 1 582.27571 1744.81257 1744.81165 0.53 0.00031 2556303.5 CID (Collision Induced Dissociation) MS2 13.11602 60 87.4 60.0 65.6258 19793 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 88.5 94.7 66.2 81.9 80.2 83.0 105.0 80.9 95.7 98.2 Excluded by Method 4.05 1 0.0004462 0.0009144 False High Sequest HT (A2) Unambiguous LHERFEQEFEDSEEEK [K].lHERFEQEFEDsEEEk.[E] N-Term(TMT6plex); S12(Phospho); K16(TMT6plex) 1 P25037 P25037 1 3 0.4866 0 1 1 873.73894 2619.20227 2619.20161 0.25 0.00022 350522.78125 CID (Collision Induced Dissociation) MS2 8.342472 0 18.0 60.0 75.1916 23378 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 6.4 19.3 24.3 6.1 14.2 31.0 8.1 19.4 37.0 14.5 Excluded by Method 3.74 1 0.0004462 0.001213 False High Sequest HT (A2) Unambiguous SSLFLPSGSSSTSLK [K].sSLFLPSGSSStSLk.[F] N-Term(TMT6plex); T12(Phospho); K15(TMT6plex) 1 P48524 P48524 0 3 0.0675 0 1 1 679.36195 2036.07131 2036.07171 -0.2 -0.00013 1488837.25 CID (Collision Induced Dissociation) MS2 0.0 60 106.3 60.0 112.7291 37506 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 31.7 91.1 170.0 37.4 84.9 197.7 38.8 95.4 207.5 108.5 Excluded by Method 2.52 1 0.0004462 0.001163 False High Sequest HT (A2) Unambiguous ALVLELDEMSNGSEIQDALEEISGQK [K].aLVLELDEmSNGsEIQDALEEISGQk.[T] N-Term(TMT6plex); M9(Oxidation); S13(Phospho); K26(TMT6plex) 2 P17695 P17695; P17695-2 0 3 0.013 0 1 1 1124.89359 3372.66621 3372.65337 3.81 0.00428 206795.578125 CID (Collision Induced Dissociation) MS2 24.22314 80 12.9 60.0 145.5811 49680 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 6.4 10.0 16.4 3.4 11.1 20.3 8.0 14.2 24.7 14.0 2.31 1 0.0004462 0.001503 False High Sequest HT (A2) Unambiguous ELSPAGSISK [K].eLsPAGSISk.[N] N-Term(TMT6plex); S3(Phospho); K10(TMT6plex) 4 O95453 O95453-4; O95453-2; O95453-3; O95453 0 2 0.2667 0 1 1 763.91499 1526.82271 1526.82315 -0.29 -0.00022 2931686.75 CID (Collision Induced Dissociation) MS2 12.96442 40 289.5 58.66 74.9394 23281 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 300.1 285.3 234.8 299.6 285.0 299.6 316.0 308.3 275.2 290.9 Excluded by Method 2.55 1 0.0004462 0.0005878 False High Sequest HT (A2) Unambiguous EDSAPVAAGESESSL [K].eDSAPVAAGEsESSL.[-] N-Term(TMT6plex); S11(Phospho) 1 P25567 P25567 0 2 0.0061 0 1 1 879.38603 1757.76478 1757.76799 -1.82 -0.0016 463703.25 CID (Collision Induced Dissociation) MS2 0.0 90 60.4 60.0 74.8525 23248 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 17.5 51.5 95.6 17.2 45.0 115.9 24.5 51.0 121.8 63.6 3.29 1 0.0004462 0.001671 False High Sequest HT (A2) Unambiguous YAAAAPAATSAASGDAAPAEEAAAEEEEESDDDMGFGLFD [K].yAAAAPAATSAASGDAAPAEEAAAEEEEEsDDDmGFGLFD.[-] N-Term(TMT6plex); S30(Phospho); M34(Oxidation) 1 P05317 P05317 0 4 0.3381 0 1 1 1062.19383 4245.75348 4245.73286 4.85 0.00515 450983.625 CID (Collision Induced Dissociation) MS2 3.44006 60 14.3 60.0 145.0347 49490 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 2.6 9.4 24.4 7.3 13.8 27.5 3.1 11.4 27.8 15.8 Excluded by Method 4.2 1 0.0004462 0.001574 False High Sequest HT (A2) Unambiguous SSLESPTMLK [K].sSLEsPTMLk.[L] N-Term(TMT6plex); S5(Phospho); K10(TMT6plex) 1 Q12044 Q12044 0 2 0.0789 0 1 1 815.9303 1630.85333 1630.85273 0.37 0.0003 1111032.75 CID (Collision Induced Dissociation) MS2 19.26939 20 81.9 60.0 88.3657 28326 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 20.1 65.0 110.2 27.0 68.8 156.5 31.6 75.7 171.1 93.1 Excluded by Method 3.17 1 0.0004462 0.001138 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVSsDEEF.[-] N-Term(TMT6plex); S9(Phospho) 1 P16521 P16521 0 2 0.0373 0 1 1 885.37175 1769.73622 1769.73562 0.34 0.0003 735765.5 CID (Collision Induced Dissociation) MS2 0.0 40 52.3 60.0 108.0312 35750 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 22.5 47.4 73.9 22.9 42.4 93.5 28.8 50.7 93.8 46.7 Excluded by Method 3.22 1 0.0004462 0.0007 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVssDEEF.[-] N-Term(TMT6plex); S8(Phospho); S9(Phospho) 1 P16521 P16521 0 2 0.1821 0 1 1 925.35581 1849.70433 1849.70195 1.29 0.00119 846471.25 CID (Collision Induced Dissociation) MS2 0.0 40 54.5 60.0 133.6612 45563 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 15.2 44.7 92.5 14.2 48.0 101.8 18.7 48.3 112.4 49.1 Excluded by Method 2.91 1 0.0004462 0.0007906 False High Sequest HT (A2) Unambiguous VQGEAVSNIQENTQTPTVQEESEEEEVDETGVEVK [K].vQGEAVSNIQENTQTPTVQEEsEEEEVDETGVEVk.[D] N-Term(TMT6plex); S22(Phospho); K35(TMT6plex) 3 Q13765 Q13765; E9PAV3; E9PAV3-2 0 4 0.0807 0 1 1 1100.52277 4399.06923 4399.06016 2.06 0.00227 158789.96875 CID (Collision Induced Dissociation) MS2 8.13855 40 8.3 60.0 143.9884 49137 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 6.6 9.3 7.6 8.9 10.0 6.2 8.8 9.3 7.5 9.1 Excluded by Method 3.47 1 0.0004462 0.0008412 False High Sequest HT (A2) Unambiguous ESITNSPTSEVPIETK [K].eSITNsPTSEVPIETk.[L] N-Term(TMT6plex); S6(Phospho); K16(TMT6plex) 1 P39521 P39521 0 3 0.0093 0 1 1 757.39065 2270.15739 2270.1569 0.22 0.00016 1757735.375 CID (Collision Induced Dissociation) MS2 1.973068 60 93.7 47.117 88.7505 28474 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 18.9 71.4 141.5 26.4 81.2 177.5 32.4 89.3 193.4 104.9 Excluded by Method 4.31 1 0.0004462 0.001227 False High Sequest HT (A2) Unambiguous EEEAAEESDDDMGFGLFD [K].eEEAAEEsDDDMGFGLFD.[-] N-Term(TMT6plex); S8(Phospho) 2 P05319 P10622; P05319 0 2 0.6706 0 1 1 1157.94897 2314.89067 2314.8784 5.3 0.00614 380651.8125 CID (Collision Induced Dissociation) MS2 40.12577 60 33.2 60.0 144.1717 49199 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 3.8 30.8 57.1 8.2 21.3 62.7 6.8 35.5 68.1 38.1 Excluded by Method 3.43 1 0.0004462 0.0006012 False High Sequest HT (A2) Unambiguous AEETPASSNESTPSASSSSSANK [K].aEETPASsNESTPSASSSSSANk.[L] N-Term(TMT6plex); S8(Phospho); K23(TMT6plex) 1 P32583 P32583 0 3 0.0109 0 1 1 922.09001 2764.25548 2764.25623 -0.27 -0.00025 2017585.75 CID (Collision Induced Dissociation) MS2 0.0 60 5.9 60.0 47.5858 13110 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 1.9 3.2 8.6 4.1 14.2 3.9 12.7 10.0 Excluded by Method 2.76 1 0.0004462 0.0006695 False High Sequest HT (A2) Unambiguous EEEAAEESDDDMGFGLFD [K].eEEAAEEsDDDMGFGLFD.[-] N-Term(TMT6plex); S8(Phospho) 2 P05319 P10622; P05319 0 3 0.7968 0 1 1 772.30046 2314.88681 2314.8784 3.63 0.0028 253094.6875 CID (Collision Induced Dissociation) MS2 60.63832 50 29.8 60.0 144.6794 49372 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 6.8 23.3 42.6 10.7 25.2 55.4 9.4 26.0 66.8 31.8 Excluded by Method 3.1 1 0.0004462 0.001241 False High Sequest HT (A2) Unambiguous NEATPEAEQVKK [K].nEAtPEAEQVkk.[E] N-Term(TMT6plex); T4(Phospho); K11(TMT6plex); K12(TMT6plex) 1 Q08245 Q08245 1 3 0.5788 0 1 1 704.38285 2111.13398 2111.13526 -0.61 -0.00043 910030.25 CID (Collision Induced Dissociation) MS2 0.0 0 123.9 60.0 54.4428 15636 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 44.7 90.9 183.3 43.8 114.3 213.1 54.3 120.0 245.4 128.9 Excluded by Method 4.44 1 0.0004462 0.001419 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVssDEEF.[-] N-Term(TMT6plex); S8(Phospho); S9(Phospho) 1 P16521 P16521 0 2 0.1347 0 1 1 925.35562 1849.70397 1849.70195 1.09 0.00101 810207.375 CID (Collision Induced Dissociation) MS2 23.94876 40 92.7 60.0 147.8535 50525 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 24.4 68.4 153.6 28.1 83.1 183.2 30.7 84.4 183.3 87.4 Excluded by Method 3.49 1 0.0004462 0.00067 False High Sequest HT (A2) Unambiguous EEEAAEESDDDMGFGLFD [K].eEEAAEESDDDmGFGLFD.[-] N-Term(TMT6plex); M12(Oxidation) 2 P05319 P10622; P05319 0 3 0.6756 0 1 1 750.97424 2250.90818 2250.90698 0.53 0.0004 754022.4375 CID (Collision Induced Dissociation) MS2 15.43047 50 38.9 60.0 114.6686 38225 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 7.1 35.0 56.8 16.0 35.9 80.9 12.1 28.6 79.9 36.8 Excluded by Method 2.99 1 0.0004462 0.000994 False High Sequest HT (A2) Unambiguous EALDQSF [K].eALDQsF.[-] N-Term(TMT6plex); S6(Phospho) 1 Q9HAS0 Q9HAS0 0 2 0.6037 0 1 1 559.75187 1118.49647 1118.49684 -0.33 -0.00018 425200.5 CID (Collision Induced Dissociation) MS2 0.0 40 74.6 60.0 84.3772 26835 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 68.3 60.7 65.5 70.9 73.8 75.5 88.1 87.0 82.5 74.0 Excluded by Method 2.17 1 0.0004462 0.001147 False High Sequest HT (A2) Unambiguous DAEDWFFSK [K].dAEDWFFSk.[T] N-Term(TMT6plex); K9(TMT6plex) 1 Q04695 Q04695 0 2 0.413 0 1 1 801.91352 1602.81977 1602.82043 -0.41 -0.00033 884043.8125 CID (Collision Induced Dissociation) MS2 14.44872 50 41.6 60.0 117.644 39324 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 39.8 43.0 34.1 50.0 39.0 47.3 34.0 39.1 42.9 46.6 Excluded by Method 2.47 1 0.0004462 0.0005673 False High Sequest HT (A2) Unambiguous IGGDAATTVNNSTPDFGFGGQK [K].iGGDAATTVNNStPDFGFGGQk.[R] N-Term(TMT6plex); T13(Phospho); K22(TMT6plex) 1 Q92945 Q92945 0 3 0.068 0 1 1 898.1044 2692.29865 2692.302 -1.24 -0.00112 750072.5 CID (Collision Induced Dissociation) MS2 0.0 40 36.6 60.0 106.4959 35185 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 34.5 33.2 30.5 44.7 29.7 37.7 38.3 37.0 40.6 39.3 Excluded by Method 4.12 1 0.0004462 0.0008275 False High Sequest HT (A2) Unambiguous LFEESDDKEDEDADGK [K].lFEEsDDkEDEDADGk.[E] N-Term(TMT6plex); S5(Phospho); K8(TMT6plex); K16(TMT6plex) 1 O43719 O43719 1 3 0.4189 0 1 1 870.40862 2609.21132 2609.21106 0.1 8e-05 2434889.5 CID (Collision Induced Dissociation) MS2 0.0 40 194.7 50.469 85.088 27101 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 192.8 172.3 129.0 258.7 190.7 157.3 248.1 211.0 210.8 176.7 Excluded by Method 2.96 1 0.0004462 0.001802 False High Sequest HT (A2) Unambiguous ALVLELDEMSNGSEIQDALEEISGQK [K].aLVLELDEmsNGSEIQDALEEISGQk.[T] N-Term(TMT6plex); M9(Oxidation); S10(Phospho); K26(TMT6plex) 2 P17695 P17695; P17695-2 0 4 0.0871 0 1 1 843.923 3372.67017 3372.65337 4.98 0.0042 1075882.75 CID (Collision Induced Dissociation) MS2 21.20506 70 41.0 24.863 132.5202 45152 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 22.7 36.9 60.7 18.1 25.2 74.4 21.5 46.2 64.4 40.2 4.02 1 0.0004462 0.000818 False High Sequest HT (A2) Unambiguous GSLVGLVDYPDDEEEDEEEESSPRK [K].gSLVGLVDYPDDEEEDEEEESsPRk.[R] N-Term(TMT6plex); S22(Phospho); K25(TMT6plex) 4 Q5MIZ7 Q5MIZ7-3; Q5MIZ7; Q5MIZ7-2; Q5MIZ7-5 1 3 0.0024 0 1 1 1121.17912 3361.52279 3361.52486 -0.61 -0.00069 1198519.625 CID (Collision Induced Dissociation) MS2 53.18789 50 23.2 60.0 105.6964 34889 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 22.5 26.6 18.1 25.8 23.0 24.0 21.9 22.3 21.6 25.9 Excluded by Method 4.14 1 0.0004462 0.001619 False High Sequest HT (A2) Unambiguous ESEEGNPVRGSEEDSPK [K].eSEEGNPVRGSEEDsPk.[K] N-Term(TMT6plex); S15(Phospho); K17(TMT6plex) 1 O43719 O43719 1 3 0.2416 0 1 1 795.37522 2384.11109 2384.1019 3.86 0.00306 317158.9375 CID (Collision Induced Dissociation) MS2 11.18251 10 111.0 60.0 45.3969 12316 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 109.4 107.1 87.7 143.4 111.2 98.6 124.0 113.1 114.6 100.8 Excluded by Method 3.89 1 0.0004462 0.001633 False High Sequest HT (A2) Unambiguous LCAGIMITASHNPK [K].lcAGImITAsHNPk.[Q] N-Term(TMT6plex); C2(Carbamidomethyl); M6(Oxidation); S10(Phospho); K14(TMT6plex) 2 Q96G03 Q96G03-2; Q96G03 0 3 0.1959 0 1 1 689.69036 2067.05653 2067.05324 1.59 0.0011 1634293.375 CID (Collision Induced Dissociation) MS2 3.098758 30 152.1 57.873 83.2275 26411 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 157.3 153.8 120.4 160.2 137.3 150.8 181.0 173.2 144.8 142.3 Excluded by Method 4.39 1 0.0004462 0.00166 False High Sequest HT (A2) Unambiguous RVTFGEDLSPEVFDESLPANTPLRK [K].rVTFGEDLsPEVFDESLPANTPLRk.[G] N-Term(TMT6plex); S9(Phospho); K25(TMT6plex) 1 Q69YH5 Q69YH5 2 4 0.1281 0 1 1 839.68918 3355.73488 3355.73394 0.28 0.00023 928182.1875 CID (Collision Induced Dissociation) MS2 43.71106 30 34.2 58.157 120.2373 40291 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 26.5 32.4 35.8 23.6 29.1 48.5 29.6 41.3 47.9 27.1 Excluded by Method 3.59 1 0.0004462 0.002154 False High Sequest HT (A2) Unambiguous DGLLGDILQDLNTETPQITPPPVMILK [K].dGLLGDILQDLNTETPQItPPPVmILk.[K] N-Term(TMT6plex); T19(Phospho); M24(Oxidation); K27(TMT6plex) 1 P09884 P09884 0 4 0.1051 0 1 1 872.22131 3485.86341 3485.86185 0.45 0.00039 1377800.625 CID (Collision Induced Dissociation) MS2 11.34058 30 13.4 60.0 131.8049 44869 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 12.1 14.2 10.1 11.9 10.4 17.6 13.7 14.4 13.3 15.8 Excluded by Method 3.14 1 0.0004462 0.001419 False High Sequest HT (A2) Unambiguous ENGSGDDLFAGLK [K].eNGsGDDLFAGLk.[K] N-Term(TMT6plex); S4(Phospho); K13(TMT6plex) 1 P09064 P09064 0 3 0.3993 0 1 1 620.97597 1860.91335 1860.91448 -0.61 -0.00038 341277.40625 CID (Collision Induced Dissociation) MS2 63.77956 30 152.8 60.0 114.0502 37996 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 35.1 121.4 241.9 50.9 137.9 274.2 58.9 145.5 297.0 165.1 Excluded by Method 2.93 1 0.0004462 0.001884 False High Sequest HT (A2) Unambiguous VLDEEGSEREFDEDSDEK [K].vLDEEGsEREFDEDsDEk.[E] N-Term(TMT6plex); S7(Phospho); S15(Phospho); K18(TMT6plex) 1 O43719 O43719 1 3 0.6388 0 1 1 916.05273 2746.14363 2746.14218 0.53 0.00048 428753.09375 CID (Collision Induced Dissociation) MS2 37.09769 40 112.0 60.0 83.9236 26667 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 117.5 113.8 84.5 125.3 100.2 111.1 114.1 122.1 113.0 117.9 Excluded by Method 4.43 1 0.0004462 0.0009841 False High Sequest HT (A2) Unambiguous EEEAAEESDDDMGFGLFD [K].eEEAAEEsDDDmGFGLFD.[-] N-Term(TMT6plex); S8(Phospho); M12(Oxidation) 2 P05319 P10622; P05319 0 2 0.5839 0 1 1 1165.9442 2330.88113 2330.87332 3.35 0.00391 1056105.375 CID (Collision Induced Dissociation) MS2 44.87049 60 63.6 60.0 145.9434 49803 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 13.2 52.1 99.2 21.7 46.6 120.3 18.4 68.0 131.6 64.8 Excluded by Method 4.11 1 0.0004462 0.0008945 False High Sequest HT (A2) Unambiguous SPAVATSTAAPPPPSSPLPSK [K].sPAVATSTAAPPPPsSPLPSk.[S] N-Term(TMT6plex); S15(Phospho); K21(TMT6plex) 5 Q14157-1 Q14157-1; Q14157-5; Q14157-4; Q14157; Q14157-3 0 4 0.0101 0 1 1 625.33833 2498.33151 2498.33078 0.29 0.00018 4374084.5 CID (Collision Induced Dissociation) MS2 1.922843 40 239.0 24.845 80.1363 25244 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 231.5 236.8 192.7 236.3 208.1 258.2 292.0 260.7 234.6 239.0 Excluded by Method 2.98 1 0.0004462 0.0008342 False High Sequest HT (A2) Unambiguous ILVALCGGN [K].iLVALcGGN.[-] N-Term(TMT6plex); C6(Carbamidomethyl) 1 P04083 P04083 0 2 0.7534 0 1 1 573.33143 1145.65558 1145.655 0.51 0.00029 438195.25 CID (Collision Induced Dissociation) MS2 0.0 100 157.0 60.0 113.4263 37766 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 151.8 162.1 145.3 143.2 151.9 172.5 167.0 174.3 155.6 146.4 2.92 1 0.0004462 0.001964 False High Sequest HT (A2) Unambiguous YAAAAPAATSAASGDAAPAEEAAAEEEEESDDDMGFGLFD [K].yAAAAPAATSAASGDAAPAEEAAAEEEEEsDDDmGFGLFD.[-] N-Term(TMT6plex); S30(Phospho); M34(Oxidation) 1 P05317 P05317 0 4 0.3527 0 1 1 1062.19395 4245.75396 4245.73286 4.97 0.00527 497086.59375 CID (Collision Induced Dissociation) MS2 15.0035 50 11.7 60.0 138.6553 47300 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 3.7 8.1 16.0 3.0 9.8 24.2 1.4 11.8 25.2 13.8 Excluded by Method 4.82 1 0.0004462 0.0007666 False High Sequest HT (A2) Unambiguous SAVCIADPLPTPSQEK [K].sAVcIADPLPTPsQEk.[S] N-Term(TMT6plex); C4(Carbamidomethyl); S13(Phospho); K16(TMT6plex) 1 Q99575 Q99575 0 3 0.1547 0 1 1 751.05347 2251.14585 2251.14456 0.58 0.00043 1414035.125 CID (Collision Induced Dissociation) MS2 7.04863 50 11.8 60.0 104.0364 34277 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 10.9 10.2 10.2 14.8 8.7 10.9 11.6 16.1 15.1 9.4 Excluded by Method 3.62 1 0.0004462 0.0005825 False High Sequest HT (A2) Unambiguous VQGEAVSNIQENTQTPTVQEESEEEEVDETGVEVK [K].vQGEAVSNIQENTQTPTVQEEsEEEEVDETGVEVk.[D] N-Term(TMT6plex); S22(Phospho); K35(TMT6plex) 3 Q13765 Q13765; E9PAV3; E9PAV3-2 0 4 0.003 0 1 1 1100.5202 4399.05898 4399.06016 -0.27 -0.0003 172355.203125 CID (Collision Induced Dissociation) MS2 25.14756 50 10.6 60.0 138.8044 47352 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 11.4 11.2 7.8 9.5 10.5 9.6 10.5 11.5 12.4 11.2 Excluded by Method 3.33 1 0.0004462 0.00104 False High Sequest HT (A2) Unambiguous ENLANNEILDN [K].eNLANNEILDN.[-] N-Term(TMT6plex) 1 P35725 P35725 0 2 0.4414 0 1 1 744.38084 1487.7544 1487.75392 0.32 0.00024 544936.0625 CID (Collision Induced Dissociation) MS2 0.0 90 38.6 60.0 79.6925 25080 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 12.1 36.9 64.0 12.2 33.7 71.6 15.9 36.0 63.7 39.7 2.56 1 0.0004462 0.00114 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVssDEEF.[-] N-Term(TMT6plex); S8(Phospho); S9(Phospho) 1 P16521 P16521 0 2 0.1358 0 1 1 925.35391 1849.70055 1849.70195 -0.76 -0.0007 887837.3125 CID (Collision Induced Dissociation) MS2 17.01146 40 53.5 60.0 134.7959 45968 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 18.4 49.4 85.5 13.0 49.1 106.6 21.6 45.3 97.5 48.5 Excluded by Method 3.46 1 0.0004462 0.00214 False High Sequest HT (A2) Unambiguous GLSPLEEEK [K].gLsPLEEEk.[L] N-Term(TMT6plex); S3(Phospho); K9(TMT6plex) 1 P31374 P31374 0 3 0.4555 0 1 1 513.94053 1539.80705 1539.80716 -0.07 -4e-05 1263273.0 CID (Collision Induced Dissociation) MS2 4.069625 40 54.9 60.0 86.5273 27643 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 14.3 47.6 82.9 21.2 47.4 99.6 21.4 54.8 102.5 57.1 Excluded by Method 3.71 1 0.0004462 0.0008072 False High Sequest HT (A2) Unambiguous ALVLELDEMSNGSEIQDALEEISGQK [K].aLVLELDEmsNGSEIQDALEEISGQk.[T] N-Term(TMT6plex); M9(Oxidation); S10(Phospho); K26(TMT6plex) 2 P17695 P17695; P17695-2 0 4 0.0944 0 1 1 843.92096 3372.66199 3372.65337 2.56 0.00216 456666.40625 CID (Collision Induced Dissociation) MS2 15.87374 70 38.0 60.0 141.0645 48163 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 12.6 32.5 58.3 13.3 33.3 64.2 18.7 36.5 71.2 39.4 4.66 1 0.0004462 0.0009636 False High Sequest HT (A2) Unambiguous VLSPLRSPPLIGSESAYESFLSADDK [K].vLsPLRsPPLIGSESAYESFLSADDk.[A] N-Term(TMT6plex); S3(Phospho); S7(Phospho); K26(TMT6plex) 1 P46821 P46821 1 4 0.1297 0 1 1 849.92586 3396.68162 3396.67814 1.02 0.00087 3018725.0 CID (Collision Induced Dissociation) MS2 0.0 60 21.7 36.305 129.7111 43989 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 16.0 18.4 17.1 23.8 24.5 22.9 23.5 21.1 29.5 19.8 Excluded by Method 4.01 1 0.0004462 0.000835 False High Sequest HT (A2) Unambiguous VTPDIEESLLEPENEK [K].vTPDIEEsLLEPENEk.[I] N-Term(TMT6plex); S8(Phospho); K16(TMT6plex) 1 Q14151 Q14151 0 3 0.0469 0 1 1 794.06984 2380.19497 2380.19367 0.54 0.00043 1105887.125 CID (Collision Induced Dissociation) MS2 7.299535 20 104.9 60.0 114.8148 38278 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 104.1 104.5 89.8 102.8 104.5 99.2 117.3 116.2 112.5 98.5 Excluded by Method 4.26 1 0.0004462 0.001496 False High Sequest HT (A2) Unambiguous GSLEEIIAEGQK [K].gSLEEIIAEGQk.[K] N-Term(TMT6plex); K12(TMT6plex) 1 P02400 P02400 0 3 0.278 0 1 1 578.00116 1731.98893 1731.98929 -0.21 -0.00012 1458785.625 CID (Collision Induced Dissociation) MS2 3.969304 80 50.3 60.0 104.2986 34375 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 25.7 35.0 74.1 25.7 40.8 87.0 26.2 49.9 80.6 57.8 3.13 1 0.0004462 0.0005718 False High Sequest HT (A2) Unambiguous AFGPGLQGGSAGSPARFTIDTK [K].aFGPGLQGGSAGsPARFTIDTk.[G] N-Term(TMT6plex); S13(Phospho); K22(TMT6plex) 2 P21333 P21333; P21333-2 1 3 0.0307 0 1 1 891.79838 2673.38057 2673.38019 0.15 0.00013 353230.53125 CID (Collision Induced Dissociation) MS2 21.98753 20 138.7 60.0 104.1869 34334 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 155.4 139.0 123.5 133.6 134.4 141.2 156.6 144.7 137.0 121.6 Excluded by Method 2.28 1 0.0004462 0.0008106 False High Sequest HT (A2) Unambiguous AANMSDDESSLIK [K].aANMsDDESSLIk.[N] N-Term(TMT6plex); S5(Phospho); K13(TMT6plex) 1 P19880 P19880 0 3 0.2326 0 1 1 640.31305 1918.92458 1918.92333 0.65 0.00042 665351.1875 CID (Collision Induced Dissociation) MS2 43.99227 40 97.7 60.0 85.9819 27437 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 25.9 76.4 145.9 30.4 73.4 185.9 37.1 103.9 185.5 112.4 Excluded by Method 2.88 1 0.0004462 0.001036 False High Sequest HT (A2) Unambiguous DISASSPNLGGILK [K].dISASsPNLGGILk.[A] N-Term(TMT6plex); S6(Phospho); K14(TMT6plex) 1 Q08548 Q08548 0 3 0.0207 0 1 1 637.35242 1910.0427 1910.04002 1.4 0.00089 323388.875 CID (Collision Induced Dissociation) MS2 13.61938 20 229.1 60.0 114.8598 38295 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 62.6 180.4 385.5 68.4 191.5 430.4 84.4 218.4 427.1 242.5 Excluded by Method 4.83 1 0.0004462 0.0007218 False High Sequest HT (A2) Unambiguous VGSTENIK [K].vGsTENIk.[H] N-Term(TMT6plex); S3(Phospho); K8(TMT6plex) 4 P27816 P27816; P27816-5; P27816-6; P27816-2 0 2 0.0785 0 1 1 693.37554 1385.74379 1385.74417 -0.27 -0.00019 4369623.5 CID (Collision Induced Dissociation) MS2 2.638372 20 88.5 50.287 62.0454 18457 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 89.4 88.5 59.5 107.0 81.8 84.7 93.6 93.8 99.8 86.4 Excluded by Method 3.31 1 0.0004462 0.002078 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVssDEEF.[-] N-Term(TMT6plex); S8(Phospho); S9(Phospho) 1 P16521 P16521 0 2 0.1483 0 1 1 925.35455 1849.70183 1849.70195 -0.07 -6e-05 418117.90625 CID (Collision Induced Dissociation) MS2 0.0 40 47.6 60.0 137.1024 46766 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 13.8 45.6 83.6 17.2 36.2 84.7 16.0 37.9 88.7 52.8 Excluded by Method 2.36 1 0.0004462 0.001601 False High Sequest HT (A2) Unambiguous DASQEESLI [K].dASQEEsLI.[-] N-Term(TMT6plex); S7(Phospho) 1 P16140 P16140 0 2 0.4708 0 1 1 650.79735 1300.58741 1300.58711 0.23 0.00015 6960446.0 CID (Collision Induced Dissociation) MS2 2.725217 40 229.3 28.888 80.3872 25337 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 58.2 185.8 370.6 63.9 194.5 432.8 85.0 208.9 451.0 242.2 Excluded by Method 2.57 1 0.0004462 0.0005976 False High Sequest HT (A2) Unambiguous ESGNNASTPSSSPEPVANPPK [K].eSGNNAsTPsSSPEPVANPPk.[M] N-Term(TMT6plex); S7(Phospho); S10(Phospho); K21(TMT6plex) 1 P53741 P53741 0 3 0.0181 0 1 1 895.74465 2685.21939 2685.22104 -0.61 -0.00055 591788.75 CID (Collision Induced Dissociation) MS2 5.925513 60 74.5 60.0 62.2652 18542 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 18.7 68.5 114.2 23.9 64.3 137.8 25.4 70.7 144.0 77.5 Excluded by Method 4.43 1 0.0004462 0.001192 False High Sequest HT (A2) Unambiguous EEEEAKEESDDDMGFGLFD [K].eEEEAkEEsDDDmGFGLFD.[-] N-Term(TMT6plex); K6(TMT6plex); S9(Phospho); M13(Oxidation) 1 P05318 P05318 1 3 0.7081 0 1 1 916.0511 2746.13874 2746.13669 0.75 0.00068 1985603.625 CID (Collision Induced Dissociation) MS2 6.289876 40 153.7 60.0 113.6813 37859 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 40.4 132.1 206.4 54.3 120.6 277.7 50.6 151.1 344.8 159.4 Excluded by Method 4.18 1 0.0004462 0.002119 False High Sequest HT (A2) Unambiguous ALVLELDEMSNGSEIQDALEEISGQK [K].aLVLELDEmsNGSEIQDALEEISGQk.[T] N-Term(TMT6plex); M9(Oxidation); S10(Phospho); K26(TMT6plex) 2 P17695 P17695; P17695-2 0 3 0.0417 0 1 1 1124.88895 3372.6523 3372.65337 -0.32 -0.00036 289676.4375 CID (Collision Induced Dissociation) MS2 47.10347 60 14.8 60.0 137.6477 46953 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 4.1 11.5 25.6 3.9 9.4 29.4 3.5 13.7 29.8 17.6 Excluded by Method 2.4 1 0.0004462 0.0006173 False High Sequest HT (A2) Unambiguous ADTTTPTTSAITASRSESPPPLSDPK [K].aDTTTPTTSAITASRSEsPPPLSDPk.[Q] N-Term(TMT6plex); S18(Phospho); K26(TMT6plex) 2 Q15059 Q15059; Q15059-2 1 3 0.1029 0 1 1 1056.20232 3166.59241 3166.59209 0.1 0.00011 363043.59375 CID (Collision Induced Dissociation) MS2 6.333346 20 92.3 60.0 80.5766 25406 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 89.1 94.6 88.1 82.2 81.3 103.9 92.5 92.2 113.8 85.3 Excluded by Method 2.72 1 0.0004462 0.001612 False High Sequest HT (A2) Unambiguous FGSNINLEADES [K].fGsNINLEADES.[-] N-Term(TMT6plex); S3(Phospho) 1 Q9NQP4 Q9NQP4 0 2 0.6364 0 1 1 802.85609 1604.7049 1604.70426 0.39 0.00032 1167441.875 CID (Collision Induced Dissociation) MS2 50.3603 80 76.8 60.0 105.5563 34839 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 76.1 76.9 63.3 85.8 70.7 71.0 81.2 95.8 81.7 65.8 2.97 1 0.0004462 0.002037 False High Sequest HT (A2) Unambiguous RSRLTPVSPESSSTEEK [K].rsRLTPVSPESSSTEEk.[S] N-Term(TMT6plex); S2(Phospho); K17(TMT6plex) 2 Q13501 Q13501-2; Q13501 2 4 0.003 0 1 1 607.81785 2428.24957 2428.2485 0.44 0.00027 2303987.0 CID (Collision Induced Dissociation) MS2 0.5600885 20 93.9 45.792 51.8127 14660 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 96.4 103.1 79.1 90.0 91.9 89.8 108.4 104.4 89.7 85.8 Excluded by Method 3.37 1 0.0004462 0.001081 False High Sequest HT (A2) Unambiguous GNVGFVFTNEPLTEIK [K].gNVGFVFTNEPLTEIk.[N] N-Term(TMT6plex); K16(TMT6plex) 1 P05317 P05317 0 3 0.2361 0 1 1 741.75166 2223.24042 2223.24254 -0.95 -0.00071 516566.46875 CID (Collision Induced Dissociation) MS2 0.0 60 39.3 60.0 126.085 42428 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 19.6 36.0 50.7 19.6 32.8 67.1 23.1 33.0 67.5 43.7 Excluded by Method 2.33 1 0.0004462 0.001923 False High Sequest HT (A2) Unambiguous FGSNINLEADES [K].fGSNINLEADES.[-] N-Term(TMT6plex) 1 Q9NQP4 Q9NQP4 0 2 0.6549 0 1 1 762.87361 1524.73995 1524.73793 1.32 0.00101 636379.8125 CID (Collision Induced Dissociation) MS2 34.88574 100 72.5 60.0 93.9742 30445 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 77.6 73.7 62.8 67.1 67.0 70.1 82.8 82.0 68.7 73.6 2.26 1 0.0004462 0.001413 False High Sequest HT (A2) Unambiguous AESDAVAEANDIDD [K].aESDAVAEANDIDD.[-] N-Term(TMT6plex) 1 P35180 P35180 0 2 0.5939 0 1 1 832.38005 1663.75282 1663.74962 1.92 0.0016 298723.34375 CID (Collision Induced Dissociation) MS2 0.0 60 57.7 60.0 67.7744 20591 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 14.6 45.9 96.9 22.5 51.0 108.0 19.0 56.4 110.6 52.0 Excluded by Method 2.93 1 0.0004462 0.0008711 False High Sequest HT (A2) Unambiguous NYGSPLISGSTPK [K].nyGSPLISGStPk.[H] N-Term(TMT6plex); Y2(Phospho); T11(Phospho); K13(TMT6plex) 1 Q15648 Q15648 0 3 0.1905 0 1 1 646.9843 1938.93835 1938.93793 0.22 0.00014 783976.625 CID (Collision Induced Dissociation) MS2 74.12579 70 55.1 60.0 89.4436 28736 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 53.2 55.4 48.1 58.2 52.3 54.5 57.2 60.9 55.9 54.9 3.57 1 0.0004462 0.001213 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVssDEEF.[-] N-Term(TMT6plex); S8(Phospho); S9(Phospho) 1 P16521 P16521 0 2 0.1405 0 1 1 925.35504 1849.70281 1849.70195 0.46 0.00043 4424168.5 CID (Collision Induced Dissociation) MS2 4.543738 30 137.3 44.535 123.6539 41535 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 33.2 112.1 215.3 39.3 116.4 258.0 39.7 126.7 284.6 148.1 Excluded by Method 3.63 1 0.0004462 0.0009145 False High Sequest HT (A2) Unambiguous LEVTEIVKPSPK [K].lEVTEIVkPsPk.[R] N-Term(TMT6plex); K8(TMT6plex); S10(Phospho); K12(TMT6plex) 2 Q7Z6E9 Q7Z6E9-2; Q7Z6E9 0 4 0.6336 0 1 1 527.5642 2107.23498 2107.23827 -1.56 -0.00082 577015.5 CID (Collision Induced Dissociation) MS2 0.0 40 236.8 60.0 101.5828 33349 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 239.9 225.3 187.1 280.6 229.1 204.2 297.0 254.4 232.9 217.4 Excluded by Method 2.62 1 0.0004462 0.0005762 False High Sequest HT (A2) Unambiguous ESEDDLNKESEEEVGPTK [K].eSEDDLNkEsEEEVGPTk.[E] N-Term(TMT6plex); K8(TMT6plex); S10(Phospho); K18(TMT6plex) 1 O43719 O43719 1 4 0.1305 0 1 1 701.34659 2802.36455 2802.35371 3.87 0.00271 600300.1875 CID (Collision Induced Dissociation) MS2 0.0 80 85.5 60.0 67.9573 20658 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 80.3 81.1 78.5 95.7 81.0 78.4 106.5 78.0 90.6 84.7 3.83 1 0.0004462 0.001021 False High Sequest HT (A2) Unambiguous GYGFVDFDSPSAAQK [K].gYGFVDFDsPSAAQk.[A] N-Term(TMT6plex); S9(Phospho); K15(TMT6plex) 1 Q15434 Q15434 0 3 0.147 0 1 1 709.67821 2127.02007 2127.02001 0.03 2e-05 1010461.6875 CID (Collision Induced Dissociation) MS2 38.75629 40 96.7 60.0 110.3968 36633 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 92.8 92.8 79.5 105.8 96.7 97.7 107.3 102.3 96.4 96.0 Excluded by Method 3.47 1 0.0004462 0.0009478 False High Sequest HT (A2) Unambiguous KEESEESDDDMGFGLFD [K].kEESEEsDDDmGFGLFD.[-] N-Term(TMT6plex); K1(TMT6plex); S7(Phospho); M11(Oxidation) 3 P05387 P05386; P05386-2; P05387 1 2 0.1219 0 1 1 1252.527 2504.04672 2504.04642 0.12 0.00015 517106.90625 CID (Collision Induced Dissociation) MS2 0.0 20 87.4 60.0 111.9379 37211 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 97.7 87.0 63.5 96.3 77.9 78.1 103.2 95.3 92.7 82.0 Excluded by Method 3.2 1 0.0004462 0.0008748 False High Sequest HT (A2) Unambiguous NMITGTSQADCAILIIAGGVGEFEAGISK [K].nMITGTSQADcAILIIAGGVGEFEAGISk.[D] N-Term(TMT6plex); C11(Carbamidomethyl); K29(TMT6plex) 1 P02994 P02994 0 4 0.3656 0 1 1 846.20135 3381.78356 3381.77983 1.1 0.00093 1837131.375 CID (Collision Induced Dissociation) MS2 5.071239 60 21.5 21.383 131.236 44590 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 9.7 17.1 28.7 11.1 17.6 38.0 10.6 22.3 38.3 21.8 Excluded by Method 3.31 1 0.0004462 0.001094 False High Sequest HT (A2) Unambiguous ALVLELDEMSNGSEIQDALEEISGQK [K].aLVLELDEmsNGSEIQDALEEISGQk.[T] N-Term(TMT6plex); M9(Oxidation); S10(Phospho); K26(TMT6plex) 2 P17695 P17695; P17695-2 0 4 0.0625 0 1 1 843.92102 3372.66224 3372.65337 2.63 0.00222 297594.375 CID (Collision Induced Dissociation) MS2 13.79661 70 34.1 60.0 139.5744 47641 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 13.3 25.5 50.5 10.9 27.9 53.6 17.6 35.3 67.7 38.5 4.32 1 0.0004462 0.00214 False High Sequest HT (A2) Unambiguous TVSPTTQISAGK [K].tVSPtTQISAGk.[S] N-Term(TMT6plex); T5(Phospho); K12(TMT6plex) 1 P53297 P53297 0 3 0.0076 0 1 1 576.65017 1727.93595 1727.93449 0.85 0.00049 1263015.75 CID (Collision Induced Dissociation) MS2 3.721341 60 77.1 60.0 70.1469 21482 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 20.1 60.9 107.5 25.1 61.2 157.2 28.5 76.8 146.6 87.3 Excluded by Method 2.63 1 0.0004462 0.001279 False High Sequest HT (A2) Unambiguous ATSTSP [K].aTSTSP.[-] N-Term(TMT6plex) 1 Q14442 Q14442 0 2 0.3125 0 1 1 396.71871 792.43014 792.43006 0.09 4e-05 63664.15625 CID (Collision Induced Dissociation) MS2 0.0 0 5.9 60.0 11.1938 1824 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 3.1 6.1 3.9 3.7 9.1 3.4 9.6 7.8 6.0 6.0 Excluded by Method 0.16 1 0.0004462 0.001313 False High Sequest HT (A2) Unambiguous LAAVPAAGPASAGGAAAASGDAAAEEEK [K].lAAVPAAGPAsAGGAAAASGDAAAEEEk.[E] N-Term(TMT6plex); S11(Phospho); K28(TMT6plex) 1 P05319 P05319 0 3 0.1872 0 1 1 973.82102 2919.44851 2919.45012 -0.55 -0.00054 533912.375 CID (Collision Induced Dissociation) MS2 0.0 40 22.2 60.0 91.8668 29654 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 11.8 22.5 22.5 11.6 23.0 38.4 13.9 21.4 29.3 27.5 Excluded by Method 2.35 1 0.0004462 0.001103 False High Sequest HT (A2) Unambiguous ALVLELDEMSNGSEIQDALEEISGQK [K].aLVLELDEMsNGSEIQDALEEISGQk.[T] N-Term(TMT6plex); S10(Phospho); K26(TMT6plex) 2 P17695 P17695; P17695-2 0 4 0.1076 0 1 1 839.92078 3356.66129 3356.65845 0.84 0.00071 13794507.0 CID (Collision Induced Dissociation) MS2 8.988886 50 42.5 4.421 131.3781 44651 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 10.1 35.7 59.9 13.9 31.7 86.8 12.6 44.9 78.8 50.4 Excluded by Method 3.16 1 0.0004462 0.001183 False High Sequest HT (A2) Unambiguous NDFEIVLSEDEK [K].nDFEIVLsEDEk.[E] N-Term(TMT6plex); S8(Phospho); K12(TMT6plex) 1 Q03654 Q03654 0 3 0.386 0 1 1 659.32651 1975.96499 1975.96658 -0.8 -0.00053 894541.875 CID (Collision Induced Dissociation) MS2 6.835067 40 46.9 60.0 112.0923 37268 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 16.6 34.8 61.3 19.2 45.6 79.6 20.1 45.5 96.1 50.0 Excluded by Method 4.56 1 0.0004462 0.001442 False High Sequest HT (A2) Unambiguous YAAAAPAATSAASGDAAPAEEAAAEEEEESDDDMGFGLFD [K].yAAAAPAATSAASGDAAPAEEAAAEEEEEsDDDmGFGLFD.[-] N-Term(TMT6plex); S30(Phospho); M34(Oxidation) 1 P05317 P05317 0 4 0.3248 0 1 1 1062.19302 4245.75025 4245.73286 4.1 0.00435 553954.6875 CID (Collision Induced Dissociation) MS2 10.17389 90 21.2 60.0 138.6321 47313 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 3.5 18.7 34.2 2.8 14.3 43.5 9.2 19.6 41.4 24.4 3.51 1 0.0004462 0.0007275 False High Sequest HT (A2) Unambiguous AGLSPAEPLLSRAGFSPDSSK [K].aGLSPAEPLLSRAGFsPDSSk.[A] N-Term(TMT6plex); S16(Phospho); K21(TMT6plex) 1 O95402 O95402 1 3 0.1649 0 1 1 875.79534 2625.37147 2625.36895 0.96 0.00084 941120.75 CID (Collision Induced Dissociation) MS2 5.80869 40 41.6 60.0 110.2249 36571 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 38.2 42.4 38.6 48.5 40.3 43.9 43.7 43.6 42.4 34.8 Excluded by Method 3.7 1 0.0004462 0.00173 False High Sequest HT (A2) Unambiguous RGRGALVDSDDE [K].rGRGALVDsDDE.[-] N-Term(TMT6plex); S9(Phospho) 1 P36053 P36053 2 3 0.4635 0 1 1 533.584 1598.73745 1598.7373 0.1 5e-05 1862692.75 CID (Collision Induced Dissociation) MS2 3.360412 10 89.1 60.0 35.9687 8921 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 24.6 71.4 136.9 24.7 68.6 164.3 38.9 84.8 187.7 88.9 Excluded by Method 2.33 1 0.0004462 0.001557 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVSsDEEF.[-] N-Term(TMT6plex); S9(Phospho) 1 P16521 P16521 0 2 0.0415 0 1 1 885.37303 1769.73878 1769.73562 1.78 0.00158 448278.75 CID (Collision Induced Dissociation) MS2 21.39665 50 73.0 60.0 125.168 42091 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 24.3 60.5 109.1 20.9 65.3 135.7 30.4 67.0 139.4 77.3 Excluded by Method 2.89 1 0.0004462 0.001511 False High Sequest HT (A2) Unambiguous NAEEESESEAEEGD [K].nAEEEsEsEAEEGD.[-] N-Term(TMT6plex); S6(Phospho); S8(Phospho) 4 Q7L1Q6 Q7L1Q6-2; Q7L1Q6-4; Q7L1Q6-3; Q7L1Q6 0 2 0.9175 0 1 1 957.32461 1913.64195 1913.6412 0.39 0.00037 253217.71875 CID (Collision Induced Dissociation) MS2 11.55648 60 14.4 60.0 39.6269 10232 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 13.7 13.1 15.1 13.8 12.5 15.1 12.7 16.4 15.5 16.1 Excluded by Method 2.91 1 0.0004462 0.001791 False High Sequest HT (A2) Unambiguous VQGEAVSNIQENTQTPTVQEESEEEEVDETGVEVK [K].vQGEAVSNIQENTQTPTVQEEsEEEEVDETGVEVk.[D] N-Term(TMT6plex); S22(Phospho); K35(TMT6plex) 3 Q13765 Q13765; E9PAV3; E9PAV3-2 0 4 0.0494 0 1 1 1100.51961 4399.05662 4399.06016 -0.81 -0.00089 224200.328125 CID (Collision Induced Dissociation) MS2 8.308307 40 9.3 60.0 139.3399 47560 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 12.0 7.5 8.7 9.2 2.1 7.6 10.2 12.7 11.7 11.5 Excluded by Method 3.44 1 0.0004462 0.001547 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVSsDEEF.[-] N-Term(TMT6plex); S9(Phospho) 1 P16521 P16521 0 2 0.024 0 1 1 885.37224 1769.73719 1769.73562 0.89 0.00079 606885.1875 CID (Collision Induced Dissociation) MS2 31.00013 50 66.5 60.0 125.0758 42055 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 19.0 49.6 109.0 19.6 50.6 131.2 30.1 62.9 124.9 68.4 Excluded by Method 3.34 1 0.0004462 0.001901 False High Sequest HT (A2) Unambiguous YAAAAPAATSAASGDAAPAEEAAAEEEEESDDDMGFGLFD [K].yAAAAPAATSAASGDAAPAEEAAAEEEEEsDDDmGFGLFD.[-] N-Term(TMT6plex); S30(Phospho); M34(Oxidation) 1 P05317 P05317 0 4 0.2524 0 1 1 1062.19497 4245.75807 4245.73286 5.94 0.0063 414425.0625 CID (Collision Induced Dissociation) MS2 0.0 50 23.9 60.0 144.3805 49314 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 4.1 19.9 39.8 3.9 14.5 44.9 8.7 18.6 60.5 24.4 Excluded by Method 4.16 1 0.0004462 0.001389 False High Sequest HT (A2) Unambiguous GEAAAERPGEAAVASSPSK [K].gEAAAERPGEAAVASSPsk.[A] N-Term(TMT6plex); S18(Phospho); K19(TMT6plex) 1 P29966 P29966 0 5 0.0207 0 1 1 465.43968 2323.16932 2323.16953 -0.09 -4e-05 1799562.375 CID (Collision Induced Dissociation) MS2 0.0 30 26.2 60.0 50.8449 14299 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 28.9 23.0 19.9 23.6 25.6 26.6 31.9 26.8 26.6 28.7 Excluded by Method 1.93 1 0.0004462 0.001544 False High Sequest HT (A2) Unambiguous DYNASASTISPPSSMEEDK [K].dYNASASTIsPPSSmEEDk.[F] N-Term(TMT6plex); S10(Phospho); M15(Oxidation); K19(TMT6plex) 1 P46821 P46821 0 3 0.2157 0 1 1 861.72474 2583.15966 2583.15737 0.89 0.00076 661280.5 CID (Collision Induced Dissociation) MS2 44.6489 20 28.0 60.0 71.6387 22044 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 25.2 29.5 20.9 28.7 26.6 25.7 32.8 32.3 27.8 30.8 Excluded by Method 4.45 1 0.0004462 0.001388 False High Sequest HT (A2) Unambiguous RSDSSGGYNLSDIIQSPSSTGLLK [K].rSDSsGGYNLSDIIQSPSSTGLLk.[S] N-Term(TMT6plex); S5(Phospho); K24(TMT6plex) 2 Q9P2D0 Q9P2D0-2; Q9P2D0 1 3 0.008 0 1 1 1007.52146 3020.54983 3020.53418 5.18 0.00521 598747.5625 CID (Collision Induced Dissociation) MS2 44.94916 90 16.2 60.0 123.1263 41342 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 17.0 14.4 17.5 16.9 13.3 21.2 17.1 16.2 14.2 14.0 3.74 1 0.0004462 0.00127 False High Sequest HT (A2) Unambiguous EEEAAEESDDDMGFGLFD [K].eEEAAEEsDDDMGFGLFD.[-] N-Term(TMT6plex); S8(Phospho) 2 P05319 P10622; P05319 0 2 0.7158 0 1 1 1157.94126 2314.87524 2314.8784 -1.36 -0.00158 167840.71875 CID (Collision Induced Dissociation) MS2 42.51352 80 17.0 60.0 148.616 50789 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 1.7 13.4 26.0 6.5 11.8 31.8 7.5 18.7 32.9 19.2 2.92 1 0.0004462 0.001268 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVssDEEF.[-] N-Term(TMT6plex); S8(Phospho); S9(Phospho) 1 P16521 P16521 0 2 0.1329 0 1 1 925.3551 1849.70293 1849.70195 0.53 0.00049 4518556.5 CID (Collision Induced Dissociation) MS2 0.0 30 93.7 60.0 124.7055 41919 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 24.0 77.3 157.3 24.8 72.5 179.9 29.1 85.2 178.3 109.0 Excluded by Method 4.14 1 0.0004462 0.0007858 False High Sequest HT (A2) Unambiguous EAAGGNDSSGATSPINPAVALE [K].eAAGGNDsSGATSPINPAVALE.[-] N-Term(TMT6plex); S8(Phospho) 3 P32004-2 P32004; P32004-3; P32004-2 0 3 0.0813 0 1 1 779.69913 2337.08283 2337.08088 0.84 0.00065 1037125.5 CID (Collision Induced Dissociation) MS2 0.0 50 47.8 60.0 110.4602 36656 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 34.6 43.5 49.9 41.6 50.2 64.1 35.4 51.2 61.8 45.8 Excluded by Method 3.32 1 0.0004462 0.0008553 False High Sequest HT (A2) Unambiguous ALVLELDEMSNGSEIQDALEEISGQK [K].aLVLELDEmsNGSEIQDALEEISGQk.[T] N-Term(TMT6plex); M9(Oxidation); S10(Phospho); K26(TMT6plex) 2 P17695 P17695; P17695-2 0 4 0.1218 0 1 1 843.92077 3372.66126 3372.65337 2.34 0.00197 395533.15625 CID (Collision Induced Dissociation) MS2 25.87328 70 41.5 60.0 144.9037 49493 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 21.2 37.9 56.3 17.6 34.4 70.5 23.0 41.6 71.1 41.5 3.86 1 0.0004462 0.001614 False High Sequest HT (A2) Unambiguous SPASDTYIVFGEAK [K].sPASDTYIVFGEAk.[I] N-Term(TMT6plex); K14(TMT6plex) 3 Q13765 Q13765; E9PAV3; E9PAV3-2 0 3 0.4748 0 1 1 648.35692 1943.05621 1943.05261 1.85 0.0012 524217.125 CID (Collision Induced Dissociation) MS2 48.93074 60 51.0 60.0 101.4088 33286 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 46.8 53.5 47.2 52.7 51.3 49.3 62.0 50.0 45.7 51.4 Excluded by Method 3.18 1 0.0004462 0.001448 False High Sequest HT (A2) Unambiguous EEEAAEESDDDMGFGLFD [K].eEEAAEEsDDDMGFGLFD.[-] N-Term(TMT6plex); S8(Phospho) 2 P05319 P10622; P05319 0 2 0.7828 0 1 1 1157.93857 2314.86987 2314.8784 -3.68 -0.00426 528350.375 CID (Collision Induced Dissociation) MS2 38.63472 50 39.0 60.0 126.5506 42525 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 10.3 31.9 63.8 12.4 34.5 71.3 14.1 29.7 83.1 38.9 Excluded by Method 3.96 1 0.0004462 0.0005746 False High Sequest HT (A2) Unambiguous EYVPLDNAEQSTSSSQETK [K].eYVPLDNAEQStSSsQETk.[E] N-Term(TMT6plex); T12(Phospho); S15(Phospho); K19(TMT6plex) 1 P33322 P33322 0 3 0.0123 0 1 1 911.07684 2731.21598 2731.21529 0.25 0.00023 1044230.75 CID (Collision Induced Dissociation) MS2 0.0 60 77.3 60.0 90.2061 29025 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 24.6 65.0 97.8 35.9 70.0 136.9 37.7 79.7 140.1 85.4 Excluded by Method 4.05 1 0.0004462 0.0008147 False High Sequest HT (A2) Unambiguous NDDSHDDLILLSAK [K].nDDsHDDLILLSAk.[K] N-Term(TMT6plex); S4(Phospho); K14(TMT6plex) 1 Q06673 Q06673 0 3 0.5227 0 1 1 698.68896 2094.05233 2094.05204 0.14 0.0001 1066232.5 CID (Collision Induced Dissociation) MS2 10.17428 60 78.7 60.0 102.4298 33666 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 25.0 67.4 125.0 25.9 68.3 146.3 32.7 66.9 149.8 79.6 Excluded by Method 3.08 1 0.0004462 0.000807 False High Sequest HT (A2) Unambiguous WFFISGSV [K].wFFISGsV.[-] N-Term(TMT6plex); S7(Phospho) 1 P00817 P00817 0 2 0.3322 0 1 1 626.30441 1251.60154 1251.60124 0.23 0.00015 10856822.0 CID (Collision Induced Dissociation) MS2 0.0 70 230.4 22.785 132.8507 45255 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 50.0 191.4 349.1 67.6 174.4 468.8 79.2 204.4 480.6 238.2 3.01 1 0.0004462 0.002007 False High Sequest HT (A2) Unambiguous TPSPAPAAK [K].tPsPAPAAk.[I] N-Term(TMT6plex); S3(Phospho); K9(TMT6plex) 1 P15891 P15891 0 2 0.3464 0 1 1 689.38073 1377.75418 1377.75434 -0.11 -8e-05 5290834.0 CID (Collision Induced Dissociation) MS2 4.759738 30 159.5 60.0 47.9693 13251 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 42.7 125.5 248.5 45.2 130.4 312.3 59.9 145.3 306.0 179.5 Excluded by Method 2.8 1 0.0004462 0.001994 False High Sequest HT (A2) Unambiguous EEEAAEESDDDMGFGLFD [K].eEEAAEEsDDDMGFGLFD.[-] N-Term(TMT6plex); S8(Phospho) 2 P05319 P10622; P05319 0 3 0.7607 0 1 1 772.29975 2314.88469 2314.8784 2.72 0.0021 382956.28125 CID (Collision Induced Dissociation) MS2 4.353138 60 25.9 60.0 141.8801 48447 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 3.1 20.0 42.6 8.9 21.9 54.9 9.6 22.6 49.9 25.5 Excluded by Method 3.51 1 0.0004462 0.0007136 False High Sequest HT (A2) Unambiguous VVDYSQFQESDDADEDYGRDSGPPTK [K].vVDYSQFQEsDDADEDYGRDSGPPTk.[K] N-Term(TMT6plex); S10(Phospho); K26(TMT6plex) 1 Q9H1E3 Q9H1E3 1 4 0.0629 0 1 1 865.38881 3458.53342 3458.53134 0.6 0.00052 313631.09375 CID (Collision Induced Dissociation) MS2 47.29639 40 6.1 60.0 138.2231 47170 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 2.5 5.8 8.1 6.3 4.2 8.4 7.6 3.8 7.4 6.9 Excluded by Method 3.02 1 0.0004462 0.001925 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVssDEEF.[-] N-Term(TMT6plex); S8(Phospho); S9(Phospho) 1 P16521 P16521 0 2 0.1471 0 1 1 925.35498 1849.70269 1849.70195 0.4 0.00037 3380686.5 CID (Collision Induced Dissociation) MS2 0.0 40 152.5 49.846 119.2057 39909 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 35.5 117.6 256.1 44.6 124.0 289.1 49.4 138.7 320.9 149.5 Excluded by Method 4.08 1 0.0004462 0.0005797 False High Sequest HT (A2) Unambiguous ALIVEPSRELAEQTLNNIK [K].aLIVEPSRELAEQTLNNIk.[Q] N-Term(TMT6plex); K19(TMT6plex) 3 Q92499 Q92499; Q92499-3; Q92499-2 1 4 0.2451 0 1 1 649.88243 2596.5079 2596.50742 0.18 0.00012 1088128.0 CID (Collision Induced Dissociation) MS2 2.187969 10 42.5 60.0 118.16 39518 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 37.5 41.5 35.1 46.6 39.9 49.5 45.3 40.9 42.3 46.2 Excluded by Method 3.59 1 0.0004462 0.0007424 False High Sequest HT (A2) Unambiguous NPDEDEFLINSDDEM [K].nPDEDEFLINsDDEm.[-] N-Term(TMT6plex); S11(Phospho); M15(Oxidation) 1 Q08412 Q08412 0 3 0.5847 0 1 1 703.28045 2107.82679 2107.82524 0.73 0.00052 607194.875 CID (Collision Induced Dissociation) MS2 15.31349 70 85.6 60.0 102.3432 33635 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 19.9 62.2 151.9 28.3 62.9 165.4 27.2 86.2 168.9 83.0 4.19 1 0.0004462 0.0006986 False High Sequest HT (A2) Unambiguous KDDSHSAEDSEDEK [K].kDDSHsAEDsEDEk.[E] N-Term(TMT6plex); K1(TMT6plex); S6(Phospho); S10(Phospho); K14(TMT6plex) 2 Q9H1E3 Q9H1E3; Q9H1E3-2 1 4 0.3181 0 1 1 610.52012 2439.05864 2439.05689 0.72 0.00044 244345.3125 CID (Collision Induced Dissociation) MS2 14.6062 40 120.9 60.0 39.1287 10058 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 124.6 111.7 84.4 149.0 116.5 113.9 157.3 124.5 113.4 113.4 Excluded by Method 5.47 1 0.0004462 0.001377 False High Sequest HT (A2) Unambiguous EEEAAEESDDDMGFGLFD [K].eEEAAEEsDDDmGFGLFD.[-] N-Term(TMT6plex); S8(Phospho); M12(Oxidation) 2 P05319 P10622; P05319 0 3 0.7855 0 1 1 777.63128 2330.87929 2330.87332 2.56 0.00199 6730160.5 CID (Collision Induced Dissociation) MS2 32.01652 20 151.1 33.449 130.5781 44307 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 39.6 127.1 239.1 41.4 123.9 291.6 58.4 144.1 291.8 154.1 Excluded by Method 3.87 1 0.0004462 0.000582 False High Sequest HT (A2) Unambiguous KLNELQNSEDSDAEDGGK [K].kLNELQNsEDsDAEDGGk.[Q] N-Term(TMT6plex); K1(TMT6plex); S8(Phospho); S11(Phospho); K18(TMT6plex) 1 Q12136 Q12136 1 3 0.5171 0 1 1 932.77329 2796.3053 2796.29449 3.87 0.0036 775862.8125 CID (Collision Induced Dissociation) MS2 0.0 60 64.8 60.0 74.7169 23198 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 17.0 57.9 88.4 23.9 61.4 114.3 24.3 66.7 127.5 66.8 Excluded by Method 4.68 1 0.0004462 0.0008613 False High Sequest HT (A2) Unambiguous DHNSEDEDEDK [K].dHNsEDEDEDk.[Y] N-Term(TMT6plex); S4(Phospho); K11(TMT6plex) 1 O95391 O95391 0 3 0.5157 0 1 1 624.26362 1870.77632 1870.77442 1.02 0.00063 93460.5625 CID (Collision Induced Dissociation) MS2 26.91139 80 21.5 60.0 28.837 6405 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 22.6 20.1 16.7 27.2 18.6 18.5 30.8 22.6 18.1 20.1 3.51 1 0.0004462 0.00127 False High Sequest HT (A2) Unambiguous EEEAKEESDDDMGFGLFD [K].eEEAkEEsDDDmGFGLFD.[-] N-Term(TMT6plex); K5(TMT6plex); S8(Phospho); M12(Oxidation) 1 P02400 P02400 1 3 0.686 0 1 1 873.03656 2617.09511 2617.0941 0.39 0.00034 2333708.0 CID (Collision Induced Dissociation) MS2 1.510307 40 119.3 42.01 119.3684 39970 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 31.6 98.8 166.8 33.4 109.4 211.6 50.7 104.1 260.1 126.6 Excluded by Method 4.14 1 0.0004462 0.000753 False High Sequest HT (A2) Unambiguous DSSVDAFEK [K].dSsVDAFEk.[E] N-Term(TMT6plex); S3(Phospho); K9(TMT6plex) 1 P09064 P09064 0 2 0.1243 0 1 1 768.3733 1535.73932 1535.73948 -0.1 -8e-05 4048857.25 CID (Collision Induced Dissociation) MS2 18.9588 50 99.6 60.0 74.3031 23042 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 30.2 81.9 146.7 37.9 87.8 181.1 46.2 91.9 189.5 103.1 Excluded by Method 3.7 1 0.0004462 0.0009633 False High Sequest HT (A2) Unambiguous EVVKPVPITSPAVSK [K].eVVkPVPITsPAVSk.[V] N-Term(TMT6plex); K4(TMT6plex); S10(Phospho); K15(TMT6plex) 1 Q96HC4 Q96HC4 0 4 0.0145 0 1 1 580.34792 2318.36984 2318.37035 -0.22 -0.00013 1318893.375 CID (Collision Induced Dissociation) MS2 3.498135 30 214.0 60.0 89.9891 28942 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 233.5 211.1 183.7 220.6 206.6 214.4 242.3 220.6 198.9 208.6 Excluded by Method 3.44 1 0.0004462 0.001549 False High Sequest HT (A2) Unambiguous YAAAAPAATSAASGDAAPAEEAAAEEEEESDDDMGFGLFD [K].yAAAAPAATSAASGDAAPAEEAAAEEEEEsDDDmGFGLFD.[-] N-Term(TMT6plex); S30(Phospho); M34(Oxidation) 1 P05317 P05317 0 4 0.3613 0 1 1 1062.19082 4245.74146 4245.73286 2.03 0.00215 2132712.75 CID (Collision Induced Dissociation) MS2 18.15451 50 10.7 15.593 130.7104 44361 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 9.0 24.8 2.3 10.0 20.5 3.1 7.6 19.9 10.1 Excluded by Method 3.82 1 0.0004462 0.002035 False High Sequest HT (A2) Unambiguous ELAEDVDDASTGDGAAK [K].eLAEDVDDAsTGDGAAk.[G] N-Term(TMT6plex); S10(Phospho); K17(TMT6plex) 1 P17890 P17890 0 3 0.0379 0 1 1 734.67767 2202.01844 2202.02152 -1.4 -0.00103 679390.8125 CID (Collision Induced Dissociation) MS2 55.22628 60 13.9 60.0 73.3782 22698 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 7.5 12.3 15.6 7.4 11.6 21.7 9.9 14.3 23.0 15.3 Excluded by Method 3.17 1 0.0004462 0.00178 False High Sequest HT (A2) Unambiguous RTADSSSSEDEEEYVVEK [K].rTADSSSsEDEEEYVVEk.[V] N-Term(TMT6plex); S8(Phospho); K18(TMT6plex) 1 P45973 P45973 1 3 0.0071 0 1 1 866.73401 2598.18747 2598.18602 0.56 0.00048 870422.4375 CID (Collision Induced Dissociation) MS2 0.0 50 117.9 60.0 67.1184 20347 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 112.0 114.3 97.4 118.3 114.5 128.2 128.7 117.7 123.3 124.7 Excluded by Method 4.25 1 0.0004462 0.00124 False High Sequest HT (A2) Unambiguous EEEAKEESDDDMGFGLFD [K].eEEAkEEsDDDmGFGLFD.[-] N-Term(TMT6plex); K5(TMT6plex); S8(Phospho); M12(Oxidation) 1 P02400 P02400 1 3 0.6391 0 1 1 873.03796 2617.09932 2617.0941 2.0 0.00174 1202580.875 CID (Collision Induced Dissociation) MS2 6.803456 30 126.6 60.0 126.5678 42606 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 34.2 115.6 196.4 47.3 109.8 217.2 45.4 109.4 265.4 125.0 Excluded by Method 3.02 1 0.0004462 0.0007785 False High Sequest HT (A2) Unambiguous EPETRYSVLNNDDYFADVSPLRATSPSK [K].ePETRYSVLNNDDYFADVSPLRATsPSk.[S] N-Term(TMT6plex); S25(Phospho); K28(TMT6plex) 1 Q1ED39 Q1ED39 2 4 0.0166 0 1 1 928.20898 3709.8141 3709.81511 -0.27 -0.00025 2489528.0 CID (Collision Induced Dissociation) MS2 3.728186 40 46.9 38.762 111.0131 36862 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 47.0 52.0 35.3 58.5 51.3 36.7 53.4 44.5 42.1 48.4 Excluded by Method 4.21 1 0.0004462 0.001588 False High Sequest HT (A2) Unambiguous IEVLDSPASK [K].iEVLDsPASk.[K] N-Term(TMT6plex); S6(Phospho); K10(TMT6plex) 1 Q8N5I9 Q8N5I9 0 2 0.1756 0 1 1 798.936 1596.86473 1596.86501 -0.18 -0.00014 1846990.375 CID (Collision Induced Dissociation) MS2 10.95572 40 206.6 60.0 91.1889 29399 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 208.3 215.0 177.6 204.8 213.6 199.2 216.9 225.5 207.9 197.3 Excluded by Method 4.1 1 0.0004462 0.001493 False High Sequest HT (A2) Unambiguous EQLSETEGSGEDEPGNDPSETTQK [K].eQLsETEGsGEDEPGNDPSETTQk.[K] N-Term(TMT6plex); S4(Phospho); S9(Phospho); K24(TMT6plex) 2 Q13107 Q13107-2; Q13107 0 4 0.5755 0 1 1 796.33939 3182.33572 3182.33396 0.55 0.00044 1378149.0 CID (Collision Induced Dissociation) MS2 0.0 50 49.5 60.0 66.8325 20241 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 47.7 54.3 41.1 52.4 58.4 45.0 57.7 48.2 43.3 46.4 Excluded by Method 4.24 1 0.0004462 0.0005934 False High Sequest HT (A2) Unambiguous STFREESPLRIK [K].sTFREEsPLRIk.[M] N-Term(TMT6plex); S7(Phospho); K12(TMT6plex) 4 Q9NYF8 Q9NYF8-3; Q9NYF8-4; Q9NYF8; Q9NYF8-2 2 3 0.358 0 1 1 667.70296 2001.09434 2001.09345 0.44 0.0003 36851088.0 CID (Collision Induced Dissociation) MS2 4.538178 30 64.1 8.845 73.2669 22657 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 61.4 78.5 58.7 63.1 64.2 57.3 64.9 69.1 57.6 66.7 Excluded by Method 4.33 1 0.0004462 0.0007787 False High Sequest HT (A2) Unambiguous KQSFDDNDSEELEDK [K].kQsFDDNDSEELEDk.[D] N-Term(TMT6plex); K1(TMT6plex); S3(Phospho); K15(TMT6plex) 1 O60841 O60841 1 3 0.1602 0 1 1 856.07679 2566.21583 2566.21648 -0.25 -0.00022 643466.3125 CID (Collision Induced Dissociation) MS2 46.44445 20 37.4 60.0 73.5319 22756 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 37.3 35.0 31.2 33.7 41.2 34.7 44.2 35.0 43.2 38.4 Excluded by Method 4.62 1 0.0004462 0.0008952 False High Sequest HT (A2) Unambiguous SFLVIVDGSPK [K].sFLVIVDGsPk.[N] N-Term(TMT6plex); S9(Phospho); K11(TMT6plex) 2 Q9H116 Q9H116; Q9H116-4 0 3 0.6398 0 1 1 567.31904 1699.94258 1699.9436 -0.6 -0.00034 303059.96875 CID (Collision Induced Dissociation) MS2 12.89765 80 26.7 60.0 124.3196 41778 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 28.3 26.4 23.7 28.2 18.2 22.9 30.9 29.7 29.5 28.8 3.22 1 0.0004462 0.0009955 False High Sequest HT (A2) Unambiguous QREMLMEDVGSEEEQEEEDEAPFQEK [K].qREmLMEDVGsEEEQEEEDEAPFQEk.[D] N-Term(TMT6plex); M4(Oxidation); S11(Phospho); K26(TMT6plex) 2 Q9H1E3 Q9H1E3; Q9H1E3-2 1 4 0.0026 0 1 1 924.65648 3695.60407 3695.6018 0.61 0.00057 4195952.0 CID (Collision Induced Dissociation) MS2 0.0 20 18.2 22.783 90.6626 29198 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 19.4 22.1 12.8 14.6 15.1 18.0 17.5 21.6 24.0 17.0 Excluded by Method 3.84 1 0.0004462 0.000853 False High Sequest HT (A2) Unambiguous EEEAAEESDDDMGFGLFD [K].eEEAAEEsDDDMGFGLFD.[-] N-Term(TMT6plex); S8(Phospho) 2 P05319 P10622; P05319 0 3 0.8006 0 1 1 772.30027 2314.88626 2314.8784 3.4 0.00262 298404.53125 CID (Collision Induced Dissociation) MS2 6.964694 60 20.2 60.0 148.0217 50520 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 3.3 19.0 28.6 8.6 16.9 39.2 4.0 20.5 40.0 22.3 Excluded by Method 3.46 1 0.0004462 0.001208 False High Sequest HT (A2) Unambiguous ESAAPASPAPSPAPSPTPAPPQK [K].eSAAPAsPAPsPAPSPTPAPPQk.[E] N-Term(TMT6plex); S7(Phospho); S11(Phospho); K23(TMT6plex) 4 Q3KQU3 Q3KQU3-3; Q3KQU3-2; Q3KQU3-4; Q3KQU3 0 3 0.0294 0 1 1 924.45225 2771.3422 2771.34585 -1.32 -0.00122 565478.5 CID (Collision Induced Dissociation) MS2 31.15517 80 40.8 60.0 69.8152 21360 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 42.2 36.7 32.4 37.2 41.0 44.6 44.4 38.2 45.5 45.5 5.1 1 0.0004462 0.0007215 False High Sequest HT (A2) Unambiguous DWRGGRTASGNIIPSSTGAAK [K].dWRGGRtASGNIIPSSTGAAk.[A] N-Term(TMT6plex); T7(Phospho); K21(TMT6plex) 3 P00359 P00360; P00359; P00358 2 3 0.0097 0 1 1 880.79417 2640.36795 2640.36593 0.76 0.00067 227074.3125 CID (Collision Induced Dissociation) MS2 37.51768 40 36.9 60.0 69.8635 21378 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 11.0 29.6 53.2 12.0 25.5 77.1 15.8 35.7 67.6 41.7 Excluded by Method 3.09 1 0.0004462 0.001667 False High Sequest HT (A2) Unambiguous NNNINSNSDDDDDEFGDFQSETSPDTTAPK [K].nNNINsNSDDDDDEFGDFQSETSPDTTAPk.[T] N-Term(TMT6plex); S6(Phospho); K30(TMT6plex) 1 Q03769 Q03769 0 4 0.2269 0 1 1 957.65753 3827.60829 3827.60815 0.04 4e-05 816831.375 CID (Collision Induced Dissociation) MS2 13.93496 70 88.4 60.0 94.3557 30588 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 28.6 71.6 131.7 24.2 72.8 157.0 35.1 86.8 189.1 87.3 2.6 1 0.0004462 0.001825 False High Sequest HT (A2) Unambiguous EIESEIDSEEELINK [K].eIESEIDsEEELINk.[K] N-Term(TMT6plex); S8(Phospho); K15(TMT6plex) 1 Q14566 Q14566 0 3 0.1647 0 1 1 772.38141 2315.12968 2315.13074 -0.46 -0.00035 994108.125 CID (Collision Induced Dissociation) MS2 7.989465 50 66.3 60.0 111.5772 37004 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 59.5 66.5 52.3 67.0 63.4 70.4 69.9 81.1 60.9 71.8 Excluded by Method 3.34 1 0.0006414 0.002333 False High Sequest HT (A2) Unambiguous TPSPAPAAK [K].tPsPAPAAk.[I] N-Term(TMT6plex); S3(Phospho); K9(TMT6plex) 1 P15891 P15891 0 3 0.0934 0 1 1 459.92286 1377.75401 1377.75434 -0.24 -0.00011 1739464.75 CID (Collision Induced Dissociation) MS2 0.0 40 159.2 60.0 48.3822 13412 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 39.6 129.9 249.0 42.2 128.6 323.2 54.3 151.8 305.4 167.8 Excluded by Method 2.89 1 0.0006414 0.002476 False High Sequest HT (A2) Unambiguous SHHANSPTAGAAK [K].sHHANsPTAGAAk.[S] N-Term(TMT6plex); S6(Phospho); K13(TMT6plex) 5 Q15555 Q15555-2; Q15555-3; Q15555-5; Q15555-4; Q15555 0 3 0.2511 0 1 1 596.30519 1786.90103 1786.90017 0.48 0.00029 59642.625 CID (Collision Induced Dissociation) MS2 0.0 70 5.9 60.0 23.0889 4552 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 6.0 3.4 3.4 3.8 7.2 6.9 6.8 7.1 7.0 7.6 2.23 1 0.0006414 0.002476 False High Sequest HT (A2) Unambiguous VEVTSEDEK [K].vEVTsEDEk.[E] N-Term(TMT6plex); S5(Phospho); K9(TMT6plex) 1 P07264 P07264 0 2 0.071 0 1 1 787.39177 1573.77626 1573.77626 0.0 0.0 40231356.0 CID (Collision Induced Dissociation) MS2 0.0 40 269.2 6.689 61.1156 18113 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 58.8 226.5 411.4 91.2 213.1 504.8 99.5 251.6 552.3 282.4 Excluded by Method 3.52 1 0.0006414 0.002276 False High Sequest HT (A2) Unambiguous VQGEAVSNIQENTQTPTVQEESEEEEVDETGVEVK [K].vQGEAVSNIQENTQTPTVQEEsEEEEVDETGVEVk.[D] N-Term(TMT6plex); S22(Phospho); K35(TMT6plex) 3 Q13765 Q13765; E9PAV3; E9PAV3-2 0 4 0.0471 0 1 1 1100.51644 4399.04392 4399.06016 -3.69 -0.00406 234957.53125 CID (Collision Induced Dissociation) MS2 7.973984 50 10.6 60.0 140.8143 48075 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 10.1 11.7 7.0 13.1 10.9 8.7 10.3 13.5 9.2 11.2 Excluded by Method 3.61 1 0.0006414 0.002489 False High Sequest HT (A2) Unambiguous EVEDKESEGEEEDEDEDLSK [K].eVEDkEsEGEEEDEDEDLSk.[Y] N-Term(TMT6plex); K5(TMT6plex); S7(Phospho); K20(TMT6plex) 2 O95218 O95218-2; O95218 1 4 0.3424 0 1 1 777.60376 3107.39319 3107.392 0.38 0.0003 453652.375 CID (Collision Induced Dissociation) MS2 31.67245 40 31.5 60.0 70.1418 21480 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 32.1 36.3 30.8 30.1 30.4 29.1 34.1 31.4 31.3 29.2 Excluded by Method 3.3 1 0.0006414 0.002339 False High Sequest HT (A2) Unambiguous EYVPLDNAEQSTSSSQETK [K].eYVPLDNAEQSTSssQETk.[E] N-Term(TMT6plex); S14(Phospho); S15(Phospho); K19(TMT6plex) 1 P33322 P33322 0 3 0.0312 0 1 1 911.07959 2731.22421 2731.21529 3.26 0.00297 448837.5 CID (Collision Induced Dissociation) MS2 75.45217 60 52.5 60.0 89.9054 28884 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 30.5 50.0 72.2 26.2 49.2 78.1 33.3 49.6 85.0 50.9 Excluded by Method 3.85 1 0.0006414 0.002599 False High Sequest HT (A2) Unambiguous WFFISGSV [K].wFFISGsV.[-] N-Term(TMT6plex); S7(Phospho) 1 P00817 P00817 0 2 0.34 0 1 1 626.30482 1251.60236 1251.60124 0.9 0.00056 1092652.75 CID (Collision Induced Dissociation) MS2 0.0 70 191.8 60.0 132.8306 45268 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 50.6 153.5 303.4 56.7 143.7 409.3 60.4 172.4 390.5 177.3 3.0 1 0.0006414 0.002241 False High Sequest HT (A2) Unambiguous IVPSYRTQSSSCEDLPSTTQPK [K].iVPSYRTQSSscEDLPSTTQPk.[G] N-Term(TMT6plex); S11(Phospho); C12(Carbamidomethyl); K22(TMT6plex) 3 O14936-4 O14936-5; O14936-4; O14936-3 1 3 0.0112 0 1 1 1007.16662 3019.4853 3019.48479 0.17 0.00017 1034893.5625 CID (Collision Induced Dissociation) MS2 18.16497 30 45.3 60.0 82.4874 26130 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 45.5 44.9 43.2 43.2 47.1 50.5 47.3 44.8 42.5 44.0 Excluded by Method 3.58 1 0.0006414 0.002191 False High Sequest HT (A2) Unambiguous FSVSSNDSQNSLK [K].fSVSSNDsQNsLk.[N] N-Term(TMT6plex); S8(Phospho); S11(Phospho); K13(TMT6plex) 1 Q06833 Q06833 0 2 0.2573 0 1 1 1015.96489 2030.92251 2030.92374 -0.6 -0.00061 636773.75 CID (Collision Induced Dissociation) MS2 0.0 20 69.8 60.0 89.744 28824 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 20.6 68.0 103.7 26.6 52.5 122.8 23.3 64.0 143.6 72.6 Excluded by Method 3.42 1 0.0006414 0.002419 False High Sequest HT (A2) Unambiguous EEEAAEESDDDMGFGLFD [K].eEEAAEEsDDDMGFGLFD.[-] N-Term(TMT6plex); S8(Phospho) 2 P05319 P10622; P05319 0 2 0.5953 0 1 1 1157.94763 2314.88799 2314.8784 4.14 0.00479 680190.25 CID (Collision Induced Dissociation) MS2 58.42055 50 35.7 52.246 135.1742 46104 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 8.2 30.6 52.8 11.9 22.7 75.4 12.4 35.2 66.2 41.6 Excluded by Method 2.99 1 0.0006414 0.002342 False High Sequest HT (A2) Unambiguous DAQRLSPIPEEVPK [K].dAQRLsPIPEEVPk.[S] N-Term(TMT6plex); S6(Phospho); K14(TMT6plex) 3 Q96T23 Q96T23; Q96T23-3; Q96T23-2 1 3 0.44 0 1 1 706.38492 2117.1402 2117.14079 -0.28 -0.0002 321631.6875 CID (Collision Induced Dissociation) MS2 59.08084 30 170.1 60.0 82.0124 25952 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 168.5 155.6 137.1 174.5 172.4 161.4 190.3 177.7 183.5 180.2 Excluded by Method 3.25 1 0.0006414 0.002454 False High Sequest HT (A2) Unambiguous LSNSSLSNLFK [K].lSNSsLSNLFk.[K] N-Term(TMT6plex); S5(Phospho); K11(TMT6plex) 1 P13259 P13259 0 3 0.0033 0 1 1 583.31813 1747.93983 1747.93957 0.15 9e-05 706355.625 CID (Collision Induced Dissociation) MS2 0.0 30 101.9 60.0 123.2132 41324 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 24.1 90.8 145.3 33.5 82.9 184.6 38.9 93.1 205.1 120.9 Excluded by Method 3.05 1 0.0006414 0.002278 False High Sequest HT (A2) Unambiguous VEVTSEDEK [K].vEVTsEDEk.[E] N-Term(TMT6plex); S5(Phospho); K9(TMT6plex) 1 P07264 P07264 0 2 0.0687 0 1 1 787.39175 1573.77622 1573.77626 -0.02 -2e-05 23602878.0 CID (Collision Induced Dissociation) MS2 0.4653654 40 361.0 12.058 61.7312 18375 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 88.7 294.1 581.2 119.5 285.3 651.1 126.9 356.7 746.8 359.9 Excluded by Method 3.64 1 0.0006414 0.002265 False High Sequest HT (A2) Unambiguous IGEGTYGVVYK [K].iGEGtyGVVYk.[AG] N-Term(TMT6plex); T5(Phospho); Y6(Phospho); K11(TMT6plex) 5 P24941 P24941; P06493; Q00526; P24941-2; P06493-2 0 3 0.1826 0 1 1 601.96278 1803.87378 1803.87354 0.13 8e-05 903671.0 CID (Collision Induced Dissociation) MS2 28.40888 50 59.4 60.0 97.5302 31752 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 56.8 59.3 48.7 57.8 57.1 64.2 62.7 64.3 69.1 54.4 Excluded by Method 2.41 1 0.0006414 0.002554 False High Sequest HT (A2) Unambiguous LEDIPVDDIDFSDLEEQYK [K].lEDIPVDDIDFsDLEEQYk.[V] N-Term(TMT6plex); S12(Phospho); K19(TMT6plex) 1 P06103 P06103 0 3 0.3548 0 1 1 941.12104 2821.34855 2821.34727 0.45 0.00043 13309712.0 CID (Collision Induced Dissociation) MS2 0.0 60 29.1 10.358 129.8271 43972 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 9.7 26.1 44.7 9.3 21.7 52.7 10.2 34.3 52.5 29.6 Excluded by Method 3.41 1 0.0006414 0.002566 False High Sequest HT (A2) Unambiguous EEEAAEESDDDMGFGLFD [K].eEEAAEEsDDDmGFGLFD.[-] N-Term(TMT6plex); S8(Phospho); M12(Oxidation) 2 P05319 P10622; P05319 0 3 0.6782 0 1 1 777.6323 2330.88234 2330.87332 3.87 0.00301 1717927.75 CID (Collision Induced Dissociation) MS2 30.09002 40 112.2 60.0 146.3162 49932 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 25.5 90.4 180.5 38.3 83.3 225.5 36.0 114.6 204.3 123.6 Excluded by Method 3.17 1 0.0006414 0.00227 False High Sequest HT (A2) Unambiguous GVHIHQAGGSPPASSTSSSSLTNDVAK [K].gVHIHQAGGsPPASSTSSSSLTNDVAk.[Q] N-Term(TMT6plex); S10(Phospho); K27(TMT6plex) 2 Q8N122 Q8N122-3; Q8N122 0 4 0.0605 0 1 1 783.39568 3130.56089 3130.55704 1.23 0.00096 748761.25 CID (Collision Induced Dissociation) MS2 0.0 10 20.2 60.0 65.5935 19780 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 15.7 20.2 17.5 21.4 21.6 18.2 25.9 25.0 18.2 18.7 Excluded by Method 2.15 1 0.0006414 0.002318 False High Sequest HT (A2) Unambiguous SLGVSPDSLEHTFSGFSQGSSIEDDQDAISNQEK [K].sLGVSPDSLEHTFSGFSQGSSIEDDQDAIsNQEk.[K] N-Term(TMT6plex); S30(Phospho); K34(TMT6plex) 1 Q05050 Q05050 0 4 0.3522 0 1 1 1038.23359 4149.91255 4149.91779 -1.26 -0.00131 405233.96875 CID (Collision Induced Dissociation) MS2 0.0 40 6.1 60.0 125.9462 42332 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 1.4 3.8 12.6 2.1 3.5 9.7 2.0 3.6 17.9 4.0 Excluded by Method 4.23 1 0.0006414 0.002491 False High Sequest HT (A2) Unambiguous DEESAEVEDVHDDVESDDEI [K].dEESAEVEDVHDDVEsDDEI.[-] N-Term(TMT6plex); S16(Phospho) 1 P53904 P53904 0 3 0.5147 0 1 1 862.34278 2585.01379 2585.01371 0.03 3e-05 1532029.125 CID (Collision Induced Dissociation) MS2 0.0 30 74.0 60.0 84.7128 27023 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 21.9 62.3 124.4 25.2 55.3 135.5 23.5 69.8 144.6 77.8 Excluded by Method 3.07 1 0.0006414 0.00254 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVssDEEF.[-] N-Term(TMT6plex); S8(Phospho); S9(Phospho) 1 P16521 P16521 0 2 0.1603 0 1 1 925.35477 1849.70226 1849.70195 0.16 0.00015 621963.0 CID (Collision Induced Dissociation) MS2 0.0 50 57.3 60.0 141.4707 48306 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 14.3 50.5 94.4 17.0 45.3 108.8 18.7 48.1 122.0 53.6 Excluded by Method 2.87 1 0.0006414 0.00248 False High Sequest HT (A2) Unambiguous WYPSEDVAALK [K].wyPSEDVAALk.[K] N-Term(TMT6plex); Y2(Phospho); K11(TMT6plex) 1 Q02326 Q02326 0 3 0.0247 0 1 1 606.31492 1816.93021 1816.92867 0.85 0.00051 802285.8125 CID (Collision Induced Dissociation) MS2 5.510079 30 125.3 60.0 116.2839 38776 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 29.2 100.8 182.7 41.4 103.2 233.4 49.0 109.3 263.3 141.0 Excluded by Method 3.64 1 0.0006414 0.002256 False High Sequest HT (A2) Unambiguous AAAYDISEDEED [K].aAAYDIsEDEED.[-] N-Term(TMT6plex); S7(Phospho) 1 P49585 P49585 0 2 0.3213 0 1 1 818.82799 1636.64871 1636.64647 1.37 0.00112 613091.0625 CID (Collision Induced Dissociation) MS2 0.0 60 63.8 60.0 77.902 24460 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 60.5 70.5 57.2 63.8 57.2 67.6 63.1 60.0 63.4 74.7 Excluded by Method 3.33 1 0.0006414 0.002277 False High Sequest HT (A2) Unambiguous LPANIVELDLHESPESVK [K].lPANIVELDLHESPEsVk.[I] N-Term(TMT6plex); S16(Phospho); K18(TMT6plex) 1 P23201 P23201 0 3 0.0024 0 1 1 843.45221 2528.34207 2528.34134 0.29 0.00024 610497.8125 CID (Collision Induced Dissociation) MS2 36.08877 40 132.4 60.0 121.7382 40760 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 32.9 96.7 211.1 41.1 114.5 255.3 46.6 146.5 233.3 146.0 Excluded by Method 4.17 1 0.0006414 0.002548 False High Sequest HT (A2) Unambiguous FNDSEGDDTEETEDYRQFRK [K].fNDsEGDDTEETEDYRQFRk.[S] N-Term(TMT6plex); S4(Phospho); K20(TMT6plex) 3 Q9NYF8 Q9NYF8-3; Q9NYF8; Q9NYF8-2 2 3 0.0417 0 1 1 1007.11637 3019.33456 3019.33587 -0.44 -0.00044 1642890.5 CID (Collision Induced Dissociation) MS2 0.0 20 66.5 60.0 77.0681 24145 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 57.9 63.3 59.9 70.1 57.8 72.4 75.0 63.2 73.5 71.4 Excluded by Method 5.04 1 0.0006414 0.002198 False High Sequest HT (A2) Unambiguous EMEVEESPEK [K].eMEVEEsPEk.[I] N-Term(TMT6plex); S7(Phospho); K10(TMT6plex) 2 Q8N3X1 Q8N3X1-2; Q8N3X1 0 2 0.3253 0 1 1 872.90954 1744.81181 1744.81165 0.09 8e-05 1293855.5 CID (Collision Induced Dissociation) MS2 0.0 30 34.8 60.0 66.3545 20104 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 36.5 36.7 27.5 37.6 27.1 39.4 42.7 34.0 35.7 30.4 Excluded by Method 2.89 1 0.0006414 0.002249 False High Sequest HT (A2) Unambiguous SQEGKSEDNLFGVEYDDEK [K].sQEGksEDNLFGVEYDDEk.[T] N-Term(TMT6plex); K5(TMT6plex); S6(Phospho); K19(TMT6plex) 1 Q08967 Q08967 1 4 0.0244 0 1 1 739.85669 2956.40494 2956.4068 -0.63 -0.00047 466173.96875 CID (Collision Induced Dissociation) MS2 8.030996 40 25.9 60.0 99.0802 32341 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 3.7 19.7 36.4 12.6 22.5 45.3 8.9 19.6 57.2 33.4 Excluded by Method 2.46 1 0.0006414 0.002532 False High Sequest HT (A2) Unambiguous VQGEAVSNIQENTQTPTVQEESEEEEVDETGVEVK [K].vQGEAVSNIQENTQTPtVQEESEEEEVDETGVEVk.[D] N-Term(TMT6plex); T17(Phospho); K35(TMT6plex) 3 Q13765 Q13765; E9PAV3; E9PAV3-2 0 4 0.0031 0 1 1 1100.51806 4399.0504 4399.06016 -2.22 -0.00244 201277.53125 CID (Collision Induced Dissociation) MS2 10.07169 50 8.9 60.0 146.4413 49988 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 7.8 8.8 10.5 8.8 6.4 9.7 10.1 11.0 8.4 7.1 Excluded by Method 3.21 1 0.0006414 0.002519 False High Sequest HT (A2) Unambiguous LVEQANSPK [K].lVEQANsPk.[H] N-Term(TMT6plex); S7(Phospho); K9(TMT6plex) 1 O95391 O95391 0 2 0.4545 0 1 1 762.41548 1523.82369 1523.82348 0.14 0.0001 2044412.25 CID (Collision Induced Dissociation) MS2 13.57284 60 131.5 60.0 62.2272 18526 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 130.6 133.9 98.1 146.7 132.5 127.5 154.5 138.6 134.7 117.5 Excluded by Method 3.63 1 0.0006414 0.002384 False High Sequest HT (A2) Unambiguous SPEEEGAVSS [K].sPEEEGAVSs.[-] N-Term(TMT6plex); S10(Phospho) 2 Q01130 Q01130-2; Q01130 0 2 0.019 0 1 1 650.77928 1300.55128 1300.55072 0.43 0.00028 1235912.625 CID (Collision Induced Dissociation) MS2 2.442931 80 190.5 60.0 40.7322 10585 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 192.3 183.6 160.4 193.4 186.5 196.6 213.8 205.1 190.1 182.9 3.15 1 0.0006414 0.00248 False High Sequest HT (A2) Unambiguous EEEAAEESDDDMGFGLFD [K].eEEAAEEsDDDmGFGLFD.[-] N-Term(TMT6plex); S8(Phospho); M12(Oxidation) 2 P05319 P10622; P05319 0 3 0.7217 0 1 1 777.63006 2330.87563 2330.87332 0.99 0.00077 16927818.0 CID (Collision Induced Dissociation) MS2 13.6956 30 57.2 5.493 128.5412 43390 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 12.6 49.5 97.3 11.4 51.5 105.3 20.1 57.8 102.9 63.4 Excluded by Method 3.45 1 0.0006414 0.002251 False High Sequest HT (A2) Unambiguous FACQIDIPNEENNNASK [K].fAcQIDIPNEENNNASk.[D] N-Term(TMT6plex); C3(Carbamidomethyl); K17(TMT6plex) 1 P06105 P06105 0 3 0.473 0 1 1 808.07355 2422.20609 2422.20729 -0.5 -0.0004 1727612.0 CID (Collision Induced Dissociation) MS2 6.959545 50 72.8 46.325 91.4161 29454 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 20.1 60.1 106.1 23.4 59.2 141.0 26.2 72.7 135.8 83.4 Excluded by Method 3.7 1 0.0006414 0.002192 False High Sequest HT (A2) Unambiguous SHDDGNIDLESDSFLK [K].sHDDGNIDLEsDSFLk.[F] N-Term(TMT6plex); S11(Phospho); K16(TMT6plex) 10 P18583 P18583; P18583-4; P18583-6; P18583-5; P18583-10; P18583-9; P18583-2; P18583-3; P18583-7; P18583-8 0 3 0.05 0 1 1 777.36975 2330.09468 2330.09536 -0.29 -0.00022 2299774.0 CID (Collision Induced Dissociation) MS2 7.973739 30 112.2 60.0 99.8445 32624 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 111.4 118.9 95.0 109.0 107.7 109.8 123.8 122.9 113.9 109.6 Excluded by Method 2.0 1 0.0006414 0.002546 False High Sequest HT (A2) Unambiguous ETQSPEQVK [K].eTQsPEQVk.[S] N-Term(TMT6plex); S4(Phospho); K9(TMT6plex) 3 Q9NYF8 Q9NYF8-3; Q9NYF8; Q9NYF8-2 0 3 0.0748 0 1 1 528.60741 1583.80769 1583.80822 -0.34 -0.00018 4301388.5 CID (Collision Induced Dissociation) MS2 3.688229 60 180.1 31.938 47.7393 13174 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 195.1 171.4 145.7 180.0 181.5 174.3 209.9 192.4 172.8 177.6 Excluded by Method 3.21 1 0.0006414 0.002368 False High Sequest HT (A2) Unambiguous SFKLSGFSFK [K].sFkLsGFSFk.[K] N-Term(TMT6plex); K3(TMT6plex); S5(Phospho); K10(TMT6plex) 1 P29966 P29966 1 3 0.236 0 1 1 639.02832 1915.0704 1915.06975 0.34 0.00022 5877935.0 CID (Collision Induced Dissociation) MS2 5.450449 20 208.7 21.876 119.8425 40145 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 221.9 204.6 177.2 230.2 201.7 190.5 248.1 228.1 195.0 189.2 Excluded by Method 3.56 1 0.0006414 0.00241 False High Sequest HT (A2) Unambiguous EEEAAEESDDDMGFGLFD [K].eEEAAEEsDDDmGFGLFD.[-] N-Term(TMT6plex); S8(Phospho); M12(Oxidation) 2 P05319 P10622; P05319 0 3 0.7737 0 1 1 777.63232 2330.88241 2330.87332 3.9 0.00303 2006840.375 CID (Collision Induced Dissociation) MS2 27.43714 30 97.0 48.742 140.4518 47917 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 26.0 72.5 147.9 25.4 76.0 197.8 34.9 89.9 199.6 100.4 Excluded by Method 3.8 1 0.0006414 0.002579 False High Sequest HT (A2) Unambiguous SAHVTVSGGTPK [K].sAHVTVSGGtPk.[G] N-Term(TMT6plex); T10(Phospho); K12(TMT6plex) 7 Q9BXS6 Q9BXS6-4; Q9BXS6-7; Q9BXS6; Q9BXS6-6; Q9BXS6-2; Q9BXS6-5; Q9BXS6-3 0 3 0.262 0 1 1 560.30227 1678.89225 1678.89296 -0.42 -0.00024 629661.9375 CID (Collision Induced Dissociation) MS2 0.0 80 27.3 60.0 46.6286 12724 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 28.5 24.5 27.0 24.4 27.3 28.2 29.7 29.0 26.8 27.2 3.32 1 0.0006414 0.002322 False High Sequest HT (A2) Unambiguous ILVALCGGN [K].iLVALcGGN.[-] N-Term(TMT6plex); C6(Carbamidomethyl) 1 P04083 P04083 0 2 0.7599 0 1 1 573.3309 1145.65452 1145.655 -0.42 -0.00024 1233089.5 CID (Collision Induced Dissociation) MS2 0.0 90 117.1 60.0 112.9547 37556 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 112.8 114.8 110.3 114.0 110.6 121.7 134.8 111.1 130.7 110.2 3.04 1 0.0006414 0.002405 False High Sequest HT (A2) Unambiguous DNLSDTEEVIVIKK [K].dNLsDTEEVIVIkk.[K] N-Term(TMT6plex); S4(Phospho); K13(TMT6plex); K14(TMT6plex) 1 Q08951 Q08951 1 3 0.0821 0 1 1 790.77573 2370.31265 2370.31362 -0.41 -0.00032 794607.4375 CID (Collision Induced Dissociation) MS2 34.44452 40 34.7 60.0 105.2607 34686 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 22.1 32.0 41.5 20.4 35.6 51.2 24.3 33.3 53.7 32.5 Excluded by Method 4.02 1 0.0006414 0.002408 False High Sequest HT (A2) Unambiguous SFEVTDPVNSSLK [K].sFEVtDPVNSSLk.[G] N-Term(TMT6plex); T5(Phospho); K13(TMT6plex) 1 P54838 P54838 0 3 0.102 0 1 1 654.33848 1961.00088 1961.0033 -1.23 -0.0008 319789.25 CID (Collision Induced Dissociation) MS2 12.88283 40 46.7 60.0 103.9532 34195 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 12.1 38.2 65.2 17.3 38.6 87.8 16.9 46.4 92.4 51.7 Excluded by Method 2.94 1 0.0006414 0.002452 False High Sequest HT (A2) Unambiguous RFIQENVFVAANHNGSPLSIK [K].rFIQENVFVAANHNGsPLSIk.[E] N-Term(TMT6plex); S16(Phospho); K21(TMT6plex) 4 P11172 P11172; P11172-3; P11172-2; P11172-4 1 4 0.2857 0 1 1 720.63932 2879.53545 2879.53333 0.73 0.00053 992709.8125 CID (Collision Induced Dissociation) MS2 6.804808 40 81.1 60.0 105.8862 34879 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 83.6 81.4 72.2 81.8 75.2 93.5 81.2 86.6 79.0 76.4 Excluded by Method 4.06 1 0.0006414 0.002405 False High Sequest HT (A2) Unambiguous VVGLSTLPEIYEK [K].vVGLSTLPEIYEk.[M] N-Term(TMT6plex); K13(TMT6plex) 1 P00330 P00330 0 3 0.2955 0 1 1 636.04822 1906.1301 1906.13014 -0.02 -1e-05 713253.6875 CID (Collision Induced Dissociation) MS2 18.37343 80 37.0 60.0 122.1527 40992 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 13.8 31.1 57.9 10.9 24.4 70.2 14.4 30.3 80.6 36.9 2.64 1 0.0006414 0.002194 False High Sequest HT (A2) Unambiguous GSQESTNTLEEIVK [K].gSQEStNtLEEIVk.[F] N-Term(TMT6plex); T6(Phospho); T8(Phospho); K14(TMT6plex) 1 P35845 P35845 0 3 0.0925 0 1 1 718.34486 2153.02003 2153.01803 0.93 0.00067 1469544.375 CID (Collision Induced Dissociation) MS2 3.432735 40 172.5 60.0 103.3077 33955 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 55.5 146.4 268.4 62.7 139.6 312.1 70.1 159.1 341.9 168.8 Excluded by Method 4.54 1 0.0006414 0.002447 False High Sequest HT (A2) Unambiguous LRTNLQPLESTQSQDF [K].lRTNLQPLESTQsQDF.[-] N-Term(TMT6plex); S13(Phospho) 1 Q9Y248 Q9Y248 1 3 0.1022 0 1 1 729.36151 2186.06998 2186.06919 0.36 0.00026 5270799.5 CID (Collision Induced Dissociation) MS2 2.344285 40 48.9 43.3 103.1972 33913 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 49.0 47.4 40.1 52.0 43.7 52.9 49.9 45.4 53.4 55.0 Excluded by Method 3.23 1 0.0006414 0.002264 False High Sequest HT (A2) Unambiguous DNLSDTEEVIVIKK [K].dNLsDTEEVIVIkk.[K] N-Term(TMT6plex); S4(Phospho); K13(TMT6plex); K14(TMT6plex) 1 Q08951 Q08951 1 3 0.0973 0 1 1 790.77609 2370.31373 2370.31362 0.05 4e-05 709322.125 CID (Collision Induced Dissociation) MS2 15.05332 50 41.3 60.0 106.12 34966 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 15.0 38.5 56.5 21.9 34.9 67.1 24.5 41.6 74.6 38.4 Excluded by Method 3.7 1 0.0006414 0.002362 False High Sequest HT (A2) Unambiguous SSDFLPIELK [K].ssDFLPIELk.[E] N-Term(TMT6plex); S2(Phospho); K10(TMT6plex) 1 P38814 P38814 0 3 0.0038 0 1 1 562.97524 1686.91116 1686.91196 -0.48 -0.00027 2536505.25 CID (Collision Induced Dissociation) MS2 0.0 60 55.9 60.0 128.1042 43262 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 23.8 46.1 84.0 23.5 46.4 99.1 30.2 48.3 99.0 59.1 Excluded by Method 2.66 1 0.0006414 0.002218 False High Sequest HT (A2) Unambiguous GSMADVPK [K].gsMADVPk.[E] N-Term(TMT6plex); S2(Phospho); K8(TMT6plex) 2 P04806 P04806; P04807 0 2 0.5362 0 1 1 671.84577 1342.68426 1342.68421 0.04 2e-05 3976178.5 CID (Collision Induced Dissociation) MS2 0.0 50 95.7 57.091 67.2894 20395 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 33.2 87.0 122.7 37.3 76.5 185.4 35.8 94.6 175.4 109.2 Excluded by Method 3.04 1 0.0006414 0.002282 False High Sequest HT (A2) Unambiguous DLFDYSPPLHK [K].dLFDYsPPLHk.[N] N-Term(TMT6plex); S6(Phospho); K11(TMT6plex) 3 Q9NYF8 Q9NYF8-3; Q9NYF8; Q9NYF8-2 0 3 0.0378 0 1 1 623.99007 1869.95566 1869.95522 0.23 0.00014 100948912.0 CID (Collision Induced Dissociation) MS2 3.689792 40 208.9 2.081 110.0762 36480 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 206.7 207.2 178.2 233.2 205.0 206.3 231.4 217.4 202.1 201.4 Excluded by Method 3.7 1 0.0006414 0.002588 False High Sequest HT (A2) Unambiguous SSVSPTAGSFGLSK [K].sSVsPTAGSFGLSk.[D] N-Term(TMT6plex); S4(Phospho); K14(TMT6plex) 1 Q07084 Q07084 0 3 0.0147 0 1 1 621.66023 1862.96615 1862.96652 -0.2 -0.00012 1425537.375 CID (Collision Induced Dissociation) MS2 21.63806 50 43.4 37.708 91.7875 29678 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 19.4 35.7 57.7 14.6 42.4 79.2 20.0 41.4 78.1 45.8 Excluded by Method 2.73 1 0.0006414 0.002375 False High Sequest HT (A2) Unambiguous ASPSCSSPTRDSSGVPVSK [K].aSPscSSPTRDSSGVPVSk.[E] N-Term(TMT6plex); S4(Phospho); C5(Carbamidomethyl); K19(TMT6plex) 1 Q8N5I9 Q8N5I9 1 3 0.0053 0 1 1 815.40168 2444.19048 2444.18928 0.49 0.0004 2910095.75 CID (Collision Induced Dissociation) MS2 0.0 20 130.7 60.0 55.8424 16155 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 126.6 131.0 108.9 131.0 122.1 140.8 142.1 137.1 134.3 133.4 Excluded by Method 3.79 1 0.0006414 0.002182 False High Sequest HT (A2) Unambiguous LSNSSLSNLFK [K].lSNsSLSNLFk.[K] N-Term(TMT6plex); S4(Phospho); K11(TMT6plex) 1 P13259 P13259 0 3 0.0028 0 1 1 583.31785 1747.939 1747.93957 -0.33 -0.00019 254034.0625 CID (Collision Induced Dissociation) MS2 55.03888 10 94.4 60.0 124.1207 41628 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 24.5 72.4 147.0 24.1 83.2 174.8 34.4 90.4 202.3 90.4 Excluded by Method 3.53 1 0.0006414 0.002535 False High Sequest HT (A2) Unambiguous IGIVEISPK [K].iGIVEIsPk.[G] N-Term(TMT6plex); S7(Phospho); K9(TMT6plex) 2 P07280 P07280; P07281 0 3 0.569 0 1 1 498.62925 1493.87318 1493.87445 -0.85 -0.00042 261981.15625 CID (Collision Induced Dissociation) MS2 27.92514 70 35.9 60.0 107.2381 35418 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 13.4 28.8 61.2 11.1 30.7 60.0 15.0 34.5 73.0 30.9 3.55 1 0.0006414 0.002233 False High Sequest HT (A2) Unambiguous ELDLFLAYYVQEQDSGQSGSPSHNDPAK [K].eLDLFLAYYVQEQDSGQSGsPSHNDPAk.[N] N-Term(TMT6plex); S20(Phospho); K28(TMT6plex) 3 O00712 O00712; O00712-4; O00712-5 0 4 0.0183 0 1 1 909.18581 3633.72141 3633.71506 1.75 0.00159 3224272.25 CID (Collision Induced Dissociation) MS2 16.28792 90 18.4 11.843 129.0842 43707 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 15.5 13.4 23.0 11.6 20.7 24.0 13.0 19.9 25.8 17.0 3.82 1 0.0006414 0.002465 False High Sequest HT (A2) Unambiguous ATISDEEIERQLK [K].aTIsDEEIERQLk.[A] N-Term(TMT6plex); S4(Phospho); K13(TMT6plex) 2 Q9UQN3 Q9UQN3; Q9UQN3-2 1 3 0.0862 0 1 1 690.70141 2070.08966 2070.08842 0.6 0.00041 5321928.0 CID (Collision Induced Dissociation) MS2 1.430722 10 80.4 19.281 89.6948 28830 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 77.0 77.0 61.8 80.3 74.7 83.1 96.4 83.6 81.0 89.1 Excluded by Method 4.41 1 0.0006414 0.002522 False High Sequest HT (A2) Unambiguous DVNPWLANESDEEHTVK [K].dVNPWLANEsDEEHTVk.[K] N-Term(TMT6plex); S10(Phospho); K17(TMT6plex) 1 Q04500 Q04500 0 4 0.144 0 1 1 631.05567 2521.20086 2521.20122 -0.14 -9e-05 1092047.125 CID (Collision Induced Dissociation) MS2 1.835001 70 67.8 60.0 93.0652 30103 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 25.1 55.7 96.4 27.0 49.0 129.7 30.3 62.6 127.4 74.6 3.75 1 0.0006414 0.002267 False High Sequest HT (A2) Unambiguous AAGPASPPEEDPERTK [K].aAGPAsPPEEDPERTk.[H] N-Term(TMT6plex); S6(Phospho); K16(TMT6plex) 1 Q96KM6 Q96KM6 1 3 0.4745 0 1 1 730.69918 2190.08299 2190.0844 -0.64 -0.00047 902264.25 CID (Collision Induced Dissociation) MS2 5.96909 50 60.2 60.0 53.4668 15299 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 60.8 68.3 42.1 64.6 62.5 59.1 63.1 65.3 62.3 54.3 Excluded by Method 4.11 1 0.0006414 0.00251 False High Sequest HT (A2) Unambiguous EQSEVSVSPRASK [K].eQSEVSVsPRASk.[H] N-Term(TMT6plex); S8(Phospho); K13(TMT6plex) 1 Q7L4I2 Q7L4I2 1 4 0.0438 0 1 1 486.25695 1942.00596 1942.00469 0.65 0.00032 1242753.875 CID (Collision Induced Dissociation) MS2 0.0 40 174.2 60.0 51.3255 14480 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 196.4 177.0 140.8 166.5 181.2 173.0 189.1 179.4 158.2 180.9 Excluded by Method 3.2 1 0.0006414 0.002228 False High Sequest HT (A2) Unambiguous YDSPVSSPITSASELGSIAK [K].yDsPVsSPITSASELGSIAk.[L] N-Term(TMT6plex); S3(Phospho); S6(Phospho); K20(TMT6plex) 1 P53890 P53890 0 3 0.1932 0 1 1 876.42828 2627.27029 2627.26587 1.68 0.00147 998035.1875 CID (Collision Induced Dissociation) MS2 9.864213 40 80.4 60.0 126.2971 42433 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 23.0 59.8 115.6 28.3 70.1 156.3 28.0 73.2 171.4 78.3 Excluded by Method 2.64 1 0.0006414 0.002383 False High Sequest HT (A2) Unambiguous TPPRWAEDQNSLLK [K].tPPRWAEDQNSLLk.[M] N-Term(TMT6plex); T1(Phospho); K14(TMT6plex) 3 P49792 P49792; A6NKT7; Q7Z3J3 1 3 0.2819 0 1 1 731.72094 2193.14828 2193.14694 0.61 0.00045 1238368.5 CID (Collision Induced Dissociation) MS2 16.02426 40 81.6 60.0 94.9783 30820 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 90.6 87.7 71.0 81.0 74.9 77.6 91.7 83.7 87.7 70.4 Excluded by Method 2.59 1 0.0006414 0.002503 False High Sequest HT (A2) Unambiguous IAASLSDDDLK [K].iAASLsDDDLk.[E] N-Term(TMT6plex); S6(Phospho); K11(TMT6plex) 1 P25389 P25389 0 3 0.1755 0 1 1 562.63041 1685.87668 1685.8763 0.22 0.00013 206982.5625 CID (Collision Induced Dissociation) MS2 68.62953 30 38.3 60.0 96.4377 31371 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 14.9 32.4 47.1 17.2 31.6 63.2 22.6 40.2 71.1 42.6 Excluded by Method 3.02 1 0.0006414 0.002433 False High Sequest HT (A2) Unambiguous SDTEGSRIGRDLPFEFGK [K].sDTEGSRIGRDLPFEFGk.[R] N-Term(TMT6plex); S1(Phospho); K18(TMT6plex) 1 Q08641 Q08641 2 4 0.2175 0 1 1 638.07489 2549.27773 2549.28014 -0.95 -0.0006 790396.5625 CID (Collision Induced Dissociation) MS2 3.813735 30 62.1 58.558 101.8411 33395 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 29.4 50.3 84.2 27.1 53.2 109.4 34.0 56.5 115.6 61.4 Excluded by Method 3.08 1 0.0006414 0.002168 False High Sequest HT (A2) Unambiguous SPAYTPQNLDSESESGSSIAEK [K].sPAYTPQNLDsESESGSSIAEk.[S] N-Term(TMT6plex); S1(Phospho); S11(Phospho); K22(TMT6plex) 1 Q15648 Q15648 0 3 0.2922 0 1 1 972.43717 2915.29697 2915.30008 -1.07 -0.00104 492889.75 CID (Collision Induced Dissociation) MS2 0.0 60 7.0 60.0 90.6188 29183 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 3.1 11.0 3.8 4.1 6.5 8.6 7.5 9.3 9.1 6.5 Excluded by Method 2.43 1 0.0006414 0.002253 False High Sequest HT (A2) Unambiguous SPEEEGAVSS [K].sPEEEGAVSs.[-] N-Term(TMT6plex); S10(Phospho) 2 Q01130 Q01130-2; Q01130 0 2 0.0315 0 1 1 650.7791 1300.55092 1300.55072 0.15 0.0001 2870652.75 CID (Collision Induced Dissociation) MS2 4.260367 80 218.1 60.0 40.7809 10648 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 229.3 216.8 181.2 200.3 196.7 215.7 241.9 243.5 218.5 236.9 3.17 1 0.0006414 0.0026 False High Sequest HT (A2) Unambiguous SVFGTPTLETANK [K].sVFGTPTLETANk.[N] N-Term(TMT6plex); S1(Phospho); K13(TMT6plex) 1 P49792 P49792 0 3 0.0585 0 1 1 635.00537 1903.00157 1902.99782 1.97 0.00125 700567.0625 CID (Collision Induced Dissociation) MS2 9.054376 80 140.7 60.0 98.7935 32320 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 138.8 144.4 116.0 143.1 133.3 146.1 167.0 143.0 135.3 140.4 3.59 1 0.0006414 0.002183 False High Sequest HT (A2) Unambiguous GGKDDEIDEEDDSEEAK [K].gGkDDEIDEEDDsEEAk.[A] N-Term(TMT6plex); K3(TMT6plex); S13(Phospho); K17(TMT6plex) 1 P32892 P32892 1 4 0.1489 0 1 1 662.80698 2648.20609 2648.20671 -0.23 -0.00015 420376.3125 CID (Collision Induced Dissociation) MS2 7.724041 60 17.0 60.0 58.4242 17130 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 13.3 16.7 15.8 9.3 12.7 28.2 14.2 15.6 25.5 18.3 Excluded by Method 2.35 1 0.0006414 0.002505 False High Sequest HT (A2) Unambiguous SDTEGSRIGRDLPFEFGK [K].sDTEGSRIGRDLPFEFGk.[R] N-Term(TMT6plex); S1(Phospho); K18(TMT6plex) 1 Q08641 Q08641 2 4 0.1616 0 1 1 638.07569 2549.28092 2549.28014 0.31 0.00019 947347.125 CID (Collision Induced Dissociation) MS2 0.0 10 56.0 60.0 102.9891 33792 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 48.3 53.8 69.1 25.1 51.6 85.0 37.9 51.6 82.3 55.7 Excluded by Method 3.28 1 0.0006414 0.002175 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVssDEEF.[-] N-Term(TMT6plex); S8(Phospho); S9(Phospho) 1 P16521 P16521 0 2 0.1719 0 1 1 925.3555 1849.70372 1849.70195 0.96 0.00088 987044.8125 CID (Collision Induced Dissociation) MS2 19.71701 50 58.3 60.0 135.9573 46377 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 16.6 42.2 92.8 14.9 49.1 116.8 19.9 46.7 124.3 60.1 Excluded by Method 2.85 1 0.0006414 0.002355 False High Sequest HT (A2) Unambiguous NSEPSARHVDSLSQRSPK [K].nSEPSARHVDSLSQRsPk.[A] N-Term(TMT6plex); S16(Phospho); K18(TMT6plex) 4 Q9UHD8 Q9UHD8-7; Q9UHD8-2; Q9UHD8-5; Q9UHD8 2 5 0.1092 0 1 1 507.46461 2533.29396 2533.29243 0.6 0.00031 117720.109375 CID (Collision Induced Dissociation) MS2 0.0 50 58.9 60.0 39.4957 10184 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 58.6 52.8 55.7 54.3 57.2 71.8 61.0 57.4 55.8 64.2 Excluded by Method 2.38 1 0.0007767 0.002819 False High Sequest HT (A2) Unambiguous ELNVSFINEK [K].eLNVsFINEk.[KR] N-Term(TMT6plex); S5(Phospho); K10(TMT6plex) 5 P49585 P49585; Q9Y5K3; Q9Y5K3-3; Q9Y5K3-2; Q9Y5K3-4 0 3 0.1753 0 1 1 577.64288 1730.9141 1730.91302 0.62 0.00036 598038.6875 CID (Collision Induced Dissociation) MS2 0.0 60 100.8 60.0 112.4638 37407 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 113.8 94.5 83.6 98.6 97.9 109.4 114.2 89.7 100.3 105.6 Excluded by Method 3.48 1 0.0007767 0.003451 False High Sequest HT (A2) Unambiguous LHERFEQEFEDSEEEK [K].lHERFEQEFEDsEEEk.[E] N-Term(TMT6plex); S12(Phospho); K16(TMT6plex) 1 P25037 P25037 1 3 0.4753 0 1 1 873.73902 2619.2025 2619.20161 0.34 0.00029 614380.125 CID (Collision Induced Dissociation) MS2 3.983814 10 14.0 60.0 76.8938 23998 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 4.1 10.3 20.4 6.7 16.6 23.9 2.6 14.1 25.8 15.3 Excluded by Method 3.24 1 0.0007767 0.003949 False High Sequest HT (A2) Unambiguous TQTGDDEDDVNEYFSTNNLEK [K].tQtGDDEDDVNEYFSTNNLEk.[T] N-Term(TMT6plex); T3(Phospho); K21(TMT6plex) 1 Q12389 Q12389 0 3 0.0037 0 1 1 991.44113 2972.30885 2972.30866 0.07 6e-05 249271.5 CID (Collision Induced Dissociation) MS2 0.0 60 13.8 60.0 106.7059 35189 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 7.6 12.4 17.7 8.6 10.4 22.9 11.8 15.5 18.1 12.5 Excluded by Method 2.69 1 0.0007767 0.002825 False High Sequest HT (A2) Unambiguous IVFDDEVSIENK [K].iVFDDEVsIENk.[E] N-Term(TMT6plex); S8(Phospho); K12(TMT6plex) 1 P46669 P46669 0 3 0.2811 0 1 1 649.33574 1945.99266 1945.9924 0.14 9e-05 835903.9375 CID (Collision Induced Dissociation) MS2 4.414342 40 59.3 60.0 114.0727 37967 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 15.9 42.5 90.6 21.6 45.9 119.3 21.9 52.8 127.0 55.0 Excluded by Method 3.7 1 0.0007767 0.003496 False High Sequest HT (A2) Unambiguous FLYGSDSELALPSSK [K].fLYGSDsELALPSSk.[R] N-Term(TMT6plex); S7(Phospho); K15(TMT6plex) 1 P45976 P45976 0 3 0.0136 0 1 1 718.03519 2152.09102 2152.09792 -3.21 -0.0023 718993.75 CID (Collision Induced Dissociation) MS2 0.0 30 124.3 60.0 126.7907 42690 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 34.7 103.1 190.0 49.5 98.0 242.1 47.4 119.9 224.7 133.4 Excluded by Method 3.69 1 0.0007767 0.003394 False High Sequest HT (A2) Unambiguous AEETPASSNESTPSASSSSSANK [K].aEETPASsNESTPSASSSSSANk.[L] N-Term(TMT6plex); S8(Phospho); K23(TMT6plex) 1 P32583 P32583 0 3 0.006 0 1 1 922.09116 2764.25894 2764.25623 0.98 0.0009 1621302.0 CID (Collision Induced Dissociation) MS2 0.0 60 15.9 60.0 48.1946 13297 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 3.4 11.8 23.2 2.5 11.3 35.8 2.9 12.9 37.1 18.3 Excluded by Method 3.32 1 0.0007767 0.002687 False High Sequest HT (A2) Unambiguous VEVTSEDEK [K].vEVTsEDEk.[E] N-Term(TMT6plex); S5(Phospho); K9(TMT6plex) 1 P07264 P07264 0 2 0.0563 0 1 1 787.39146 1573.77563 1573.77626 -0.39 -0.00031 27406854.0 CID (Collision Induced Dissociation) MS2 0.3364411 30 325.5 10.541 61.9995 18418 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 82.1 256.7 495.7 104.2 284.3 601.6 115.0 303.3 684.5 327.3 Excluded by Method 3.55 1 0.0007767 0.003798 False High Sequest HT (A2) Unambiguous NAEEESESEAEEGD [K].nAEEEsEsEAEEGD.[-] N-Term(TMT6plex); S6(Phospho); S8(Phospho) 4 Q7L1Q6 Q7L1Q6-2; Q7L1Q6-4; Q7L1Q6-3; Q7L1Q6 0 2 0.955 0 1 1 957.32416 1913.64104 1913.6412 -0.09 -8e-05 213615.96875 CID (Collision Induced Dissociation) MS2 0.0 50 10.8 60.0 38.9059 9976 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 8.4 11.5 10.5 11.2 9.7 12.1 12.3 10.7 10.7 11.0 Excluded by Method 2.89 1 0.0007767 0.00276 False High Sequest HT (A2) Unambiguous EEEAAEESDDDMGFGLFD [K].eEEAAEEsDDDMGFGLFD.[-] N-Term(TMT6plex); S8(Phospho) 2 P05319 P10622; P05319 0 3 0.7138 0 1 1 772.30113 2314.88883 2314.8784 4.5 0.00348 234785.046875 CID (Collision Induced Dissociation) MS2 77.10278 50 30.1 60.0 146.7235 50071 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 7.4 21.7 45.2 13.1 22.3 63.8 9.6 29.3 60.5 28.0 Excluded by Method 2.83 1 0.0007767 0.003261 False High Sequest HT (A2) Unambiguous ESEEGNPVRGSEEDSPK [K].eSEEGNPVRGSEEDsPk.[K] N-Term(TMT6plex); S15(Phospho); K17(TMT6plex) 1 O43719 O43719 1 3 0.231 0 1 1 795.37325 2384.10521 2384.1019 1.39 0.0011 915105.6875 CID (Collision Induced Dissociation) MS2 12.99274 10 81.0 60.0 46.1089 12534 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 89.3 82.2 69.2 99.1 68.9 70.8 95.6 77.6 84.3 72.8 Excluded by Method 3.68 1 0.0007767 0.00345 False High Sequest HT (A2) Unambiguous RFVSEGDGGRLKPESY [K].rFVsEGDGGRLkPESY.[-] N-Term(TMT6plex); S4(Phospho); K12(TMT6plex) 2 Q53EL6 Q53EL6-2; Q53EL6 2 4 0.3462 0 1 1 584.55145 2335.18396 2335.18478 -0.35 -0.00021 1097029.875 CID (Collision Induced Dissociation) MS2 10.79736 10 67.3 60.0 73.2833 22720 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 61.6 71.7 60.2 71.2 61.1 72.9 78.2 61.0 66.8 68.5 Excluded by Method 2.6 1 0.0007767 0.002726 False High Sequest HT (A2) Unambiguous QLVAGNSPK [K].qLVAGNsPk.[K] N-Term(TMT6plex); S7(Phospho); K9(TMT6plex) 2 Q96EU6 Q96EU6-2; Q96EU6 0 3 0.3771 0 1 1 484.60593 1451.80325 1451.80235 0.62 0.0003 859262.375 CID (Collision Induced Dissociation) MS2 15.03238 30 48.0 60.0 60.3922 17816 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 43.8 51.3 36.6 49.3 46.2 52.7 48.5 52.3 47.1 52.2 Excluded by Method 3.5 1 0.0007767 0.00403 False High Sequest HT (A2) Unambiguous ELSQILDQFEINSDGEE [K].eLSQILDQFEINsDGEE.[-] N-Term(TMT6plex); S13(Phospho) 1 P53923 P53923 0 3 0.4337 0 1 1 759.01434 2275.02846 2275.02163 3.0 0.00227 2022730.5 CID (Collision Induced Dissociation) MS2 5.429371 50 52.7 60.0 131.8987 44909 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 29.9 47.8 75.6 26.2 39.3 95.5 31.2 42.6 86.3 52.4 Excluded by Method 2.49 1 0.0007767 0.003367 False High Sequest HT (A2) Unambiguous VLDEEGSEREFDEDSDEKEEEEDTYEK [K].vLDEEGsEREFDEDsDEkEEEEDTYEk.[V] N-Term(TMT6plex); S7(Phospho); S15(Phospho); K18(TMT6plex); K27(TMT6plex) 1 O43719 O43719 2 4 0.2816 0 1 1 1032.69381 4127.7534 4127.75099 0.58 0.0006 464542.96875 CID (Collision Induced Dissociation) MS2 0.0 50 21.9 60.0 91.5496 29534 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 21.0 20.6 15.3 20.9 21.4 17.8 24.8 27.6 26.6 23.0 Excluded by Method 4.19 1 0.0007767 0.003882 False High Sequest HT (A2) Unambiguous KIESFGSPK [K].kIESFGsPk.[G] N-Term(TMT6plex); K1(TMT6plex); S7(Phospho); K9(TMT6plex) 1 P49792 P49792 1 3 0.3028 0 1 1 587.33678 1759.9958 1759.99625 -0.26 -0.00015 716575.125 CID (Collision Induced Dissociation) MS2 0.0 20 151.1 60.0 73.5218 22812 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 129.4 149.0 117.8 163.6 152.6 163.4 158.2 157.8 172.1 146.9 Excluded by Method 2.84 1 0.0007767 0.004011 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVSsDEEF.[-] N-Term(TMT6plex); S9(Phospho) 1 P16521 P16521 0 2 0.0302 0 1 1 885.37248 1769.73768 1769.73562 1.16 0.00103 638185.875 CID (Collision Induced Dissociation) MS2 45.39455 60 49.2 60.0 124.8103 41881 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 17.7 44.7 74.6 13.1 39.6 91.0 18.3 48.8 94.2 49.9 Excluded by Method 2.98 1 0.0007767 0.004038 False High Sequest HT (A2) Unambiguous YAAAAPAATSAASGDAAPAEEAAAEEEEESDDDMGFGLFD [K].yAAAAPAATSAASGDAAPAEEAAAEEEEEsDDDmGFGLFD.[-] N-Term(TMT6plex); S30(Phospho); M34(Oxidation) 1 P05317 P05317 0 4 0.25 0 1 1 1062.19242 4245.74787 4245.73286 3.53 0.00375 637861.3125 CID (Collision Induced Dissociation) MS2 14.41707 40 18.5 60.0 134.1538 45730 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 2.9 19.0 25.3 3.1 13.6 37.5 8.2 15.2 39.4 20.4 Excluded by Method 2.84 1 0.0007767 0.002897 False High Sequest HT (A2) Unambiguous GYALVPIIVNSSTPK [K].gYALVPIIVNSStPk.[S] N-Term(TMT6plex); T13(Phospho); K15(TMT6plex) 2 P82094 P82094; P82094-2 0 3 0.0505 0 1 1 699.73052 2097.17702 2097.17611 0.43 0.0003 373431.28125 CID (Collision Induced Dissociation) MS2 6.357055 40 34.5 60.0 126.516 42587 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 29.7 38.2 25.2 36.7 33.1 34.2 33.9 36.2 43.4 34.6 Excluded by Method 2.77 1 0.0007767 0.003442 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVssDEEF.[-] N-Term(TMT6plex); S8(Phospho); S9(Phospho) 1 P16521 P16521 0 2 0.1575 0 1 1 925.3549 1849.70252 1849.70195 0.31 0.00028 989903.6875 CID (Collision Induced Dissociation) MS2 23.33951 50 49.5 60.0 135.748 46291 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 11.2 37.6 81.4 11.7 40.1 86.7 17.9 48.5 103.8 55.9 Excluded by Method 2.92 1 0.0007767 0.003457 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVssDEEF.[-] N-Term(TMT6plex); S8(Phospho); S9(Phospho) 1 P16521 P16521 0 2 0.1563 0 1 1 925.35474 1849.7022 1849.70195 0.13 0.00012 3821744.25 CID (Collision Induced Dissociation) MS2 0.0 30 90.8 39.253 126.8871 42728 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 20.4 71.5 144.7 29.5 83.4 178.0 26.8 78.7 178.6 96.3 Excluded by Method 3.39 1 0.0007767 0.003102 False High Sequest HT (A2) Unambiguous ELSPAALEK [K].eLsPAALEk.[R] N-Term(TMT6plex); S3(Phospho); K9(TMT6plex) 1 O75683 O75683 0 3 0.3789 0 1 1 499.27771 1495.81858 1495.81733 0.84 0.00042 1228606.25 CID (Collision Induced Dissociation) MS2 4.266358 60 351.4 60.0 89.4367 28733 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 378.6 361.8 319.4 351.0 318.3 321.2 406.1 368.4 351.1 337.7 Excluded by Method 3.8 1 0.0007767 0.003592 False High Sequest HT (A2) Unambiguous SATPPSSITAAK [K].sAtPPSSITAAk.[T] N-Term(TMT6plex); T3(Phospho); K12(TMT6plex) 1 Q06440 Q06440 0 3 0.0929 0 1 1 556.97098 1668.89837 1668.89737 0.6 0.00033 494733.25 CID (Collision Induced Dissociation) MS2 31.46819 60 59.4 60.0 68.9097 20994 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 32.5 50.8 88.2 28.1 56.9 89.1 38.7 56.9 95.5 57.5 Excluded by Method 2.26 1 0.0007767 0.002974 False High Sequest HT (A2) Unambiguous ILVALCGGN [K].iLVALcGGN.[-] N-Term(TMT6plex); C6(Carbamidomethyl) 1 P04083 P04083 0 2 0.7653 0 1 1 573.33081 1145.65434 1145.655 -0.57 -0.00033 323984.84375 CID (Collision Induced Dissociation) MS2 0.0 80 144.6 60.0 113.858 37853 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 128.9 130.5 143.5 132.9 125.2 162.4 146.7 153.7 178.8 143.7 2.94 1 0.0007767 0.00318 False High Sequest HT (A2) Unambiguous VGEDEDGSDEEVVHNEDIHFEPIVSLPEVEVK [K].vGEDEDGsDEEVVHNEDIHFEPIVSLPEVEVk.[S] N-Term(TMT6plex); S8(Phospho); K32(TMT6plex) 1 P49792 P49792 0 4 0.243 0 1 1 1032.99239 4128.94773 4128.95787 -2.46 -0.00253 1531415.375 CID (Collision Induced Dissociation) MS2 13.28391 30 36.8 19.984 127.465 42921 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 30.8 44.6 31.1 40.4 32.4 32.8 41.6 40.8 37.8 35.5 Excluded by Method 3.91 1 0.0007767 0.003123 False High Sequest HT (A2) Unambiguous SPSLSPSPPSPLEK [K].sPSLSPSPPsPLEk.[T] N-Term(TMT6plex); S10(Phospho); K14(TMT6plex) 1 P46821 P46821 0 3 0.4208 0 1 1 654.35161 1961.04026 1961.03968 0.3 0.00019 4749043.5 CID (Collision Induced Dissociation) MS2 0.8924639 40 131.8 22.954 89.3325 28752 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 142.6 128.9 104.5 146.2 120.8 134.2 155.9 141.5 113.9 129.9 Excluded by Method 4.04 1 0.0007767 0.003511 False High Sequest HT (A2) Unambiguous RSASYTGAKV [K].rSAsYTGAkV.[-] N-Term(TMT6plex); S4(Phospho); K9(TMT6plex) 1 P31688 P31688 2 3 0.1146 0 1 1 526.62002 1577.8455 1577.84528 0.14 7e-05 905170.75 CID (Collision Induced Dissociation) MS2 15.33927 70 52.4 60.0 49.968 13950 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 23.3 39.5 70.8 24.5 45.9 92.0 29.6 51.8 91.9 54.4 2.88 1 0.0007767 0.003158 False High Sequest HT (A2) Unambiguous DEAIPELVEGQTFDADVE [K].dEAIPELVEGQTFDADVE.[-] N-Term(TMT6plex) 1 Q02642 Q02642 0 3 0.4211 0 1 1 736.02473 2206.05963 2206.06005 -0.19 -0.00014 576880.0 CID (Collision Induced Dissociation) MS2 22.51359 50 41.6 60.0 127.0034 42697 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 17.7 31.8 63.8 14.5 39.3 70.4 22.4 42.2 71.5 42.7 Excluded by Method 3.04 1 0.0007767 0.003076 False High Sequest HT (A2) Unambiguous EEEAAEESDDDMGFGLFD [K].eEEAAEEsDDDMGFGLFD.[-] N-Term(TMT6plex); S8(Phospho) 2 P05319 P10622; P05319 0 2 0.6905 0 1 1 1157.94922 2314.89116 2314.8784 5.51 0.00638 319611.4375 CID (Collision Induced Dissociation) MS2 24.53202 40 37.2 60.0 145.5326 49664 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 9.2 29.7 56.2 14.1 29.0 76.2 12.5 36.7 69.4 38.9 Excluded by Method 3.36 1 0.0007767 0.003872 False High Sequest HT (A2) Unambiguous VGEDEDGSDEEVVHNEDIHFEPIVSLPEVEVK [K].vGEDEDGsDEEVVHNEDIHFEPIVSLPEVEVk.[S] N-Term(TMT6plex); S8(Phospho); K32(TMT6plex) 1 P49792 P49792 0 4 0.4009 0 1 1 1032.99464 4128.95672 4128.95787 -0.28 -0.00029 4355848.5 CID (Collision Induced Dissociation) MS2 63.86455 30 35.8 17.016 127.4312 42951 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 38.8 31.9 28.1 36.0 31.4 43.0 38.7 33.6 40.3 36.4 Excluded by Method 4.34 1 0.0007767 0.003142 False High Sequest HT (A2) Unambiguous TPPRWAEDQNSLLK [K].tPPRWAEDQNSLLk.[M] N-Term(TMT6plex); T1(Phospho); K14(TMT6plex) 3 P49792 P49792; A6NKT7; Q7Z3J3 1 3 0.4519 0 1 1 731.72029 2193.14632 2193.14694 -0.28 -0.00021 1449903.625 CID (Collision Induced Dissociation) MS2 0.0 40 93.5 60.0 95.8953 31133 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 108.7 97.4 80.5 98.8 85.1 95.2 101.3 102.0 82.5 83.4 Excluded by Method 3.12 1 0.0007767 0.00361 False High Sequest HT (A2) Unambiguous KEPEESVEENTSK [K].kEPEESVEENTsk.[T] N-Term(TMT6plex); K1(TMT6plex); S12(Phospho); K13(TMT6plex) 1 Q01477 Q01477 1 3 0.0218 0 1 1 758.38848 2273.15089 2273.1517 -0.35 -0.00027 587291.5625 CID (Collision Induced Dissociation) MS2 44.92122 40 77.7 60.0 55.5255 16063 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 23.8 68.4 112.1 26.4 73.2 130.1 35.2 74.5 166.7 67.1 Excluded by Method 3.21 1 0.0007767 0.002797 False High Sequest HT (A2) Unambiguous NLSNENCSFK [K].nLsNENcSFk.[K] N-Term(TMT6plex); S3(Phospho); C7(Carbamidomethyl); K10(TMT6plex) 1 P11972 P11972 0 3 0.4759 0 1 1 584.2796 1750.82424 1750.82356 0.39 0.00023 3082978.0 CID (Collision Induced Dissociation) MS2 39.63843 30 82.9 60.0 70.34 21617 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 36.4 71.3 104.6 46.6 71.6 135.3 43.9 82.4 152.6 84.2 Excluded by Method 3.53 1 0.0007767 0.002624 False High Sequest HT (A2) Unambiguous RVGDEGNAAESESDNVAASRQDSTSK [K].rVGDEGNAAESESDNVAASRQDsTSk.[L] N-Term(TMT6plex); S23(Phospho); K26(TMT6plex) 1 P23615 P23615 2 4 0.0612 0 1 1 805.38007 3218.49845 3218.49629 0.67 0.00054 312373.8125 CID (Collision Induced Dissociation) MS2 43.41189 50 11.0 60.0 48.6015 13445 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 12.4 8.0 11.4 6.6 8.5 15.1 10.3 10.9 14.8 12.1 Excluded by Method 3.76 1 0.0007767 0.002941 False High Sequest HT (A2) Unambiguous TPSPAPAAK [K].tPsPAPAAk.[I] N-Term(TMT6plex); S3(Phospho); K9(TMT6plex) 1 P15891 P15891 0 2 0.3251 0 1 1 689.38095 1377.75463 1377.75434 0.21 0.00014 1328344.875 CID (Collision Induced Dissociation) MS2 19.27585 40 222.5 60.0 48.6038 13446 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 57.9 187.9 339.8 69.4 187.8 431.6 73.6 218.7 422.3 235.7 Excluded by Method 2.83 1 0.0007767 0.002813 False High Sequest HT (A2) Unambiguous SDTEGSRIGRDLPFEFGK [K].sDTEGSRIGRDLPFEFGk.[R] N-Term(TMT6plex); S1(Phospho); K18(TMT6plex) 1 Q08641 Q08641 2 4 0.24 0 1 1 638.07532 2549.27944 2549.28014 -0.28 -0.00018 611214.4375 CID (Collision Induced Dissociation) MS2 4.053372 40 54.6 60.0 102.435 33667 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 40.4 47.2 64.5 38.2 44.4 74.8 36.5 48.4 94.4 56.9 Excluded by Method 2.5 1 0.0007767 0.00345 False High Sequest HT (A2) Unambiguous DEAIPELVEGQTFDADVE [K].dEAIPELVEGQTFDADVE.[-] N-Term(TMT6plex) 1 Q02642 Q02642 0 3 0.3974 0 1 1 736.02455 2206.05911 2206.06005 -0.43 -0.00032 576113.625 CID (Collision Induced Dissociation) MS2 39.57455 50 48.9 60.0 126.9555 42754 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 22.8 45.3 57.1 20.5 38.8 92.1 26.2 47.4 88.1 50.5 Excluded by Method 3.12 1 0.0007767 0.003165 False High Sequest HT (A2) Unambiguous RVSLEPHQGPGTPESK [K].rVSLEPHQGPGtPESk.[K] N-Term(TMT6plex); T12(Phospho); K16(TMT6plex) 3 Q14980 Q14980-5; Q14980-2; Q14980 1 4 0.2167 0 1 1 565.04905 2257.17436 2257.17422 0.06 4e-05 4489232.5 CID (Collision Induced Dissociation) MS2 1.816066 30 75.8 22.994 53.0246 15109 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 52.2 71.8 92.0 46.2 73.9 113.0 53.0 72.4 112.4 70.8 Excluded by Method 2.03 1 0.0007767 0.00411 False High Sequest HT (A2) Unambiguous VQGEAVSNIQENTQTPTVQEESEEEEVDETGVEVK [K].vQGEAVSNIQENTQTPTVQEESEEEEVDEtGVEVk.[D] N-Term(TMT6plex); T30(Phospho); K35(TMT6plex) 3 Q13765 Q13765; E9PAV3; E9PAV3-2 0 4 0.0078 0 1 1 1100.52034 4399.05955 4399.06016 -0.14 -0.00015 244536.5625 CID (Collision Induced Dissociation) MS2 19.98914 40 11.0 60.0 138.0497 47109 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 9.1 11.3 8.3 9.9 12.1 12.3 11.8 13.5 12.7 9.2 Excluded by Method 3.86 1 0.0007767 0.003318 False High Sequest HT (A2) Unambiguous AAADALSDLEIK [K].aAADALsDLEIk.[D] N-Term(TMT6plex); S7(Phospho); K12(TMT6plex) 1 P09938 P09938 0 3 0.0574 0 1 1 585.64953 1754.93403 1754.93415 -0.07 -4e-05 344952.78125 CID (Collision Induced Dissociation) MS2 0.0 30 40.0 60.0 115.5671 38548 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 13.3 34.1 62.9 14.6 30.0 67.5 21.3 38.3 79.6 38.2 Excluded by Method 2.44 1 0.0007767 0.00262 False High Sequest HT (A2) Unambiguous VISAGRIIYVDDGVLSFQVLEVVDDK [K].vISAGRIIYVDDGVLSFQVLEVVDDk.[T] N-Term(TMT6plex); K26(TMT6plex) 1 P00549 P00549 1 4 0.1479 0 1 1 827.71917 3307.85484 3307.85536 -0.16 -0.00013 4782092.0 CID (Collision Induced Dissociation) MS2 9.500142 50 50.6 13.38 129.5851 43887 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 48.1 44.8 53.6 48.9 46.7 53.5 52.3 53.6 51.1 53.6 Excluded by Method 1.69 1 0.0007767 0.002866 False High Sequest HT (A2) Unambiguous DFVALSDDEDEDGK [K].dFVALsDDEDEDGk.[Q] N-Term(TMT6plex); S6(Phospho); K14(TMT6plex) 1 P25558 P25558 0 3 0.4007 0 1 1 698.31728 2092.93728 2092.9364 0.42 0.00029 1249995.125 CID (Collision Induced Dissociation) MS2 26.06241 50 88.9 60.0 94.8377 30736 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 37.7 78.5 123.3 52.7 77.5 144.2 56.8 83.0 146.9 88.6 Excluded by Method 2.67 1 0.0007767 0.002907 False High Sequest HT (A2) Unambiguous GYPVATPQK [K].gYPVAtPQk.[S] N-Term(TMT6plex); T6(Phospho); K9(TMT6plex) 1 P36421 P36421 0 3 0.4465 0 1 1 500.27405 1498.80759 1498.8071 0.33 0.00016 751352.4375 CID (Collision Induced Dissociation) MS2 49.42787 60 46.2 60.0 65.8874 19889 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 18.9 37.7 74.7 17.3 38.3 82.6 18.9 45.8 78.7 49.4 Excluded by Method 3.18 1 0.0007767 0.00345 False High Sequest HT (A2) Unambiguous VDIPNEDFDFQSNNAK [K].vDIPNEDFDFQSNNAk.[F] N-Term(TMT6plex); K16(TMT6plex) 1 P45978 P45978 0 3 0.2937 0 1 1 771.05854 2311.16106 2311.16066 0.17 0.00013 474673.5 CID (Collision Induced Dissociation) MS2 56.17192 40 20.4 60.0 102.7472 33741 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 10.7 19.5 30.8 11.7 14.2 27.9 12.7 22.2 33.6 21.1 Excluded by Method 2.69 1 0.0007767 0.00297 False High Sequest HT (A2) Unambiguous DLDEEGSEK [K].dLDEEGsEk.[E] N-Term(TMT6plex); S7(Phospho); K9(TMT6plex) 1 O43719 O43719 0 2 0.4757 0 1 1 780.36582 1559.72435 1559.72422 0.09 7e-05 18941960.0 CID (Collision Induced Dissociation) MS2 7.995687 30 172.4 41.55 55.3241 15987 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 169.4 167.2 123.5 208.6 169.1 161.5 200.9 167.1 177.1 180.0 Excluded by Method 3.91 1 0.0007767 0.003309 False High Sequest HT (A2) Unambiguous FGSNINLEADES [K].fGSNINLEADES.[-] N-Term(TMT6plex) 1 Q9NQP4 Q9NQP4 0 2 0.5648 0 1 1 762.87258 1524.73788 1524.73793 -0.03 -3e-05 663644.4375 CID (Collision Induced Dissociation) MS2 15.3248 80 36.6 60.0 95.0094 30800 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 38.6 36.1 31.1 38.7 32.4 38.8 38.3 37.4 34.9 39.3 1.93 1 0.0007767 0.002909 False High Sequest HT (A2) Unambiguous TRESEPLSK [K].tREsEPLSk.[S] N-Term(TMT6plex); S4(Phospho); K9(TMT6plex) 1 O60669 O60669 1 3 0.3184 0 1 1 528.95137 1584.83957 1584.83986 -0.18 -0.0001 1745250.25 CID (Collision Induced Dissociation) MS2 7.571207 30 101.3 60.0 49.3073 13705 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 105.1 94.3 87.0 100.8 94.5 105.0 116.9 103.7 100.6 104.9 Excluded by Method 3.58 1 0.0007767 0.002918 False High Sequest HT (A2) Unambiguous DDSHSAEDSEDEK [K].dDSHsAEDsEDEk.[E] N-Term(TMT6plex); S5(Phospho); S9(Phospho); K13(TMT6plex) 2 Q9H1E3 Q9H1E3; Q9H1E3-2 0 3 0.5417 0 1 1 694.60425 2081.79819 2081.79899 -0.38 -0.00027 85383.75 CID (Collision Induced Dissociation) MS2 0.0 20 46.3 60.0 34.0808 8243 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 41.2 43.3 35.4 57.4 47.7 46.9 52.4 53.0 45.0 40.4 Excluded by Method 3.6 1 0.0007767 0.002945 False High Sequest HT (A2) Unambiguous DFVALSDDEDEDGK [K].dFVALsDDEDEDGk.[Q] N-Term(TMT6plex); S6(Phospho); K14(TMT6plex) 1 P25558 P25558 0 3 0.5884 0 1 1 698.31614 2092.93387 2092.9364 -1.21 -0.00084 590615.3125 CID (Collision Induced Dissociation) MS2 61.44246 40 90.1 60.0 93.9932 30451 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 36.4 81.9 114.9 44.4 83.2 143.6 45.2 83.4 164.4 103.2 Excluded by Method 3.62 1 0.0007767 0.002665 False High Sequest HT (A2) Unambiguous EHPFDCNGPTQIYRSESTEVDVDGNAIREIK [K].eHPFDcNGPTQIYRSEsTEVDVDGNAIREIk.[T] N-Term(TMT6plex); C6(Carbamidomethyl); S17(Phospho); K31(TMT6plex) 1 P37303 P37303 2 4 0.0312 0 1 1 1029.49557 4114.96044 4114.95816 0.56 0.00057 704448.9375 CID (Collision Induced Dissociation) MS2 0.0 50 35.2 60.0 96.2415 31299 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 7.5 27.8 50.2 14.2 29.9 66.5 13.1 32.8 69.3 40.3 Excluded by Method 4.16 1 0.0007767 0.003119 False High Sequest HT (A2) Unambiguous NSSQQDNSSRESPSLEDEETK [K].nSSQQDNSSRESPsLEDEETk.[K] N-Term(TMT6plex); S14(Phospho); K21(TMT6plex) 2 Q96T23 Q96T23; Q96T23-2 1 4 0.0045 0 1 1 727.08356 2905.31241 2905.31005 0.81 0.00059 683882.5 CID (Collision Induced Dissociation) MS2 6.03565 30 26.7 60.0 50.1878 14058 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 25.2 25.8 18.4 31.2 26.4 25.4 32.2 28.3 29.0 25.2 Excluded by Method 4.44 1 0.0007767 0.003372 False High Sequest HT (A2) Unambiguous SVTFNEPENESSSQDVDELVK [K].sVTFNEPENESsSQDVDELVk.[D] N-Term(TMT6plex); S12(Phospho); K21(TMT6plex) 1 P39936 P39936 0 3 0.0113 0 1 1 964.45797 2891.35935 2891.35996 -0.21 -0.0002 944704.8125 CID (Collision Induced Dissociation) MS2 68.19547 30 34.2 60.0 109.0234 36053 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 11.1 27.3 48.3 14.0 27.1 64.2 18.5 30.1 65.8 35.8 Excluded by Method 3.55 1 0.0007767 0.003205 False High Sequest HT (A2) Unambiguous IMATPEQVGK [K].imAtPEQVGk.[M] N-Term(TMT6plex); M2(Oxidation); T4(Phospho); K10(TMT6plex) 2 P12956 P12956; P12956-2 0 3 0.5068 0 1 1 543.28888 1627.8521 1627.85307 -0.59 -0.00032 873118.6875 CID (Collision Induced Dissociation) MS2 72.71255 40 141.4 52.839 68.5422 20876 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 143.2 135.0 124.8 142.1 134.4 141.6 143.7 159.0 143.0 147.2 Excluded by Method 3.65 1 0.0007767 0.002962 False High Sequest HT (A2) Unambiguous GSEDSPPK [K].gSEDsPPk.[H] N-Term(TMT6plex); S5(Phospho); K8(TMT6plex) 3 P48651 P48651; P48651-3; P48651-2 0 2 0.3583 0 1 1 677.83636 1354.66544 1354.66558 -0.11 -7e-05 345437.03125 CID (Collision Induced Dissociation) MS2 9.434533 50 196.0 60.0 40.2028 10393 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 197.2 202.8 146.8 224.8 183.3 183.1 227.4 217.1 190.7 186.7 Excluded by Method 2.54 1 0.0007767 0.003337 False High Sequest HT (A2) Unambiguous EEEEAKEESDDDMGFGLFD [K].eEEEAkEEsDDDmGFGLFD.[-] N-Term(TMT6plex); K6(TMT6plex); S9(Phospho); M13(Oxidation) 1 P05318 P05318 1 3 0.6314 0 1 1 916.05067 2746.13746 2746.13669 0.28 0.00026 2124865.0 CID (Collision Induced Dissociation) MS2 2.057974 40 142.6 55.437 116.3556 38840 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 38.1 111.4 212.4 50.1 116.8 262.2 52.9 130.1 302.8 148.7 Excluded by Method 3.69 1 0.0007767 0.003749 False High Sequest HT (A2) Unambiguous NAEEESESEAEEGD [K].nAEEEsEsEAEEGD.[-] N-Term(TMT6plex); S6(Phospho); S8(Phospho) 4 Q7L1Q6 Q7L1Q6-2; Q7L1Q6-4; Q7L1Q6-3; Q7L1Q6 0 2 0.9312 0 1 1 957.32474 1913.64221 1913.6412 0.52 0.0005 382405.84375 CID (Collision Induced Dissociation) MS2 31.93681 50 23.5 60.0 40.3614 10450 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 12.7 20.9 30.5 22.1 21.6 30.6 20.9 19.9 30.6 25.5 Excluded by Method 2.76 1 0.0007767 0.003484 False High Sequest HT (A2) Unambiguous VQGEAVSNIQENTQTPTVQEESEEEEVDETGVEVK [K].vQGEAVSNIQENTQTPtVQEESEEEEVDETGVEVk.[D] N-Term(TMT6plex); T17(Phospho); K35(TMT6plex) 3 Q13765 Q13765; E9PAV3; E9PAV3-2 0 4 0.0115 0 1 1 1100.51507 4399.03847 4399.06016 -4.93 -0.00542 992945.875 CID (Collision Induced Dissociation) MS2 22.97253 40 22.4 60.0 111.1733 36921 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 22.6 17.6 19.5 22.4 20.5 27.6 25.9 23.0 19.6 25.1 Excluded by Method 4.33 1 0.0007767 0.002897 False High Sequest HT (A2) Unambiguous HIVSNDSSDSDDESHEPK [K].hIVSNDSSDsDDESHEPk.[G] N-Term(TMT6plex); S10(Phospho); K18(TMT6plex) 1 P47712 P47712 0 4 0.1531 0 1 1 634.78687 2536.12567 2536.12409 0.62 0.00039 167374.546875 CID (Collision Induced Dissociation) MS2 0.0 10 33.5 60.0 34.6407 8389 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 43.3 36.0 29.2 43.2 33.0 26.6 34.0 38.4 17.5 33.9 Excluded by Method 2.09 1 0.0007767 0.003159 False High Sequest HT (A2) Unambiguous LFEDDDSNEK [K].lFEDDDsNEk.[L] N-Term(TMT6plex); S7(Phospho); K10(TMT6plex) 1 O43719 O43719 0 3 0.3259 0 1 1 583.93705 1749.7966 1749.79845 -1.05 -0.00061 291359.5 CID (Collision Induced Dissociation) MS2 13.85125 60 43.3 60.0 89.5049 28737 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 41.6 37.5 30.7 49.8 49.2 40.0 47.6 42.6 49.3 44.4 Excluded by Method 2.24 1 0.0007767 0.002917 False High Sequest HT (A2) Unambiguous EEEAAEESDDDMGFGLFD [K].eEEAAEEsDDDmGFGLFD.[-] N-Term(TMT6plex); S8(Phospho); M12(Oxidation) 2 P05319 P10622; P05319 0 3 0.7007 0 1 1 777.63171 2330.88057 2330.87332 3.11 0.00242 8556921.0 CID (Collision Induced Dissociation) MS2 4.801038 40 70.3 10.342 126.2821 42427 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 13.9 55.5 109.1 24.8 47.6 134.9 23.1 57.7 159.8 76.3 Excluded by Method 2.74 1 0.0007767 0.00429 False High Sequest HT (A2) Unambiguous ETTSDNEDLLIK [K].eTtSDNEDLLIk.[E] N-Term(TMT6plex); T3(Phospho); K12(TMT6plex) 1 Q04347 Q04347 0 3 0.0058 0 1 1 639.32641 1915.96469 1915.96658 -0.98 -0.00063 8773217.0 CID (Collision Induced Dissociation) MS2 19.12215 30 129.7 12.804 94.5182 30649 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 37.4 104.6 193.3 42.4 101.9 239.8 47.6 136.1 258.4 135.3 Excluded by Method 3.44 1 0.0007767 0.0042 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVSsDEEF.[-] N-Term(TMT6plex); S9(Phospho) 1 P16521 P16521 0 2 0.0241 0 1 1 885.37083 1769.73439 1769.73562 -0.7 -0.00062 600139.0625 CID (Collision Induced Dissociation) MS2 0.0 60 66.2 60.0 126.2691 42495 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 24.9 58.2 104.6 22.0 49.3 129.5 28.4 56.8 122.7 65.6 Excluded by Method 2.91 1 0.0007767 0.003275 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVssDEEF.[-] N-Term(TMT6plex); S8(Phospho); S9(Phospho) 1 P16521 P16521 0 2 0.2314 0 1 1 925.3552 1849.70313 1849.70195 0.64 0.00059 419352.84375 CID (Collision Induced Dissociation) MS2 35.04718 30 52.0 60.0 141.3654 48229 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 12.2 38.0 86.9 13.4 41.4 95.0 24.6 45.0 107.0 56.5 Excluded by Method 2.42 1 0.0007767 0.002944 False High Sequest HT (A2) Unambiguous TRESEPLSK [K].tREsEPLSk.[S] N-Term(TMT6plex); S4(Phospho); K9(TMT6plex) 1 O60669 O60669 1 3 0.2584 0 1 1 528.95126 1584.83922 1584.83986 -0.41 -0.00021 2165381.5 CID (Collision Induced Dissociation) MS2 6.179856 30 116.9 60.0 48.4682 13434 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 113.8 116.2 91.0 117.5 112.5 124.6 138.4 121.7 113.2 120.6 Excluded by Method 3.56 1 0.0007767 0.003433 False High Sequest HT (A2) Unambiguous EMEVEESPEK [K].emEVEEsPEk.[I] N-Term(TMT6plex); M2(Oxidation); S7(Phospho); K10(TMT6plex) 2 Q8N3X1 Q8N3X1-2; Q8N3X1 0 3 0.5867 0 1 1 587.60699 1760.8064 1760.80657 -0.09 -6e-05 3221008.75 CID (Collision Induced Dissociation) MS2 39.33357 50 70.7 11.639 51.3627 14510 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 65.9 62.7 61.2 74.2 69.1 68.1 78.6 81.2 75.6 70.5 Excluded by Method 3.46 1 0.0007767 0.0028 False High Sequest HT (A2) Unambiguous EEEAKEESDDDMGFGLFD [K].eEEAkEEsDDDmGFGLFD.[-] N-Term(TMT6plex); K5(TMT6plex); S8(Phospho); M12(Oxidation) 1 P02400 P02400 1 3 0.5411 0 1 1 873.03704 2617.09658 2617.0941 0.95 0.00083 1346212.0 CID (Collision Induced Dissociation) MS2 0.0 60 151.1 60.0 123.0061 41296 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 37.0 119.6 216.5 52.2 117.2 289.6 60.9 144.7 313.1 159.9 Excluded by Method 2.92 1 0.0007767 0.00277 False High Sequest HT (A2) Unambiguous EEEAAEESDDDMGFGLFD [K].eEEAAEEsDDDmGFGLFD.[-] N-Term(TMT6plex); S8(Phospho); M12(Oxidation) 2 P05319 P10622; P05319 0 3 0.6427 0 1 1 777.63293 2330.88424 2330.87332 4.69 0.00364 2306169.75 CID (Collision Induced Dissociation) MS2 28.90849 40 127.7 41.55 139.3157 47522 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 27.6 103.0 201.4 34.1 97.4 247.5 54.5 126.1 258.2 126.8 Excluded by Method 3.75 1 0.0007767 0.003542 False High Sequest HT (A2) Unambiguous ISPDLNIK [K].isPDLNIk.[C] N-Term(TMT6plex); S2(Phospho); K8(TMT6plex) 1 Q69YH5 Q69YH5 0 2 0.2564 0 1 1 719.40931 1437.81135 1437.81185 -0.35 -0.00025 1273903.0 CID (Collision Induced Dissociation) MS2 27.74858 20 83.8 60.0 99.5105 32526 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 82.6 75.4 78.6 91.7 78.1 88.8 89.8 79.7 83.3 89.6 Excluded by Method 3.12 1 0.0007767 0.003607 False High Sequest HT (A2) Unambiguous SELCSSPLGK [K].sELcSsPLGk.[R] N-Term(TMT6plex); C4(Carbamidomethyl); S6(Phospho); K10(TMT6plex) 1 Q9Y2F5 Q9Y2F5 0 2 0.0071 0 1 1 808.41235 1615.81743 1615.81668 0.46 0.00037 4291410.0 CID (Collision Induced Dissociation) MS2 14.57131 20 135.1 56.78 76.3695 23801 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 117.2 132.2 115.1 129.3 123.9 153.6 144.7 144.4 153.0 137.5 Excluded by Method 2.81 1 0.0007767 0.002885 False High Sequest HT (A2) Unambiguous EDSEWQASLRK [K].eDSEWQAsLRk.[S] N-Term(TMT6plex); S8(Phospho); K11(TMT6plex) 2 Q9H788 Q9H788; Q9H788-2 1 3 0.4007 0 1 1 629.65212 1886.9418 1886.94136 0.23 0.00015 2211201.25 CID (Collision Induced Dissociation) MS2 2.987672 20 92.4 60.0 72.0773 22184 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 89.5 82.7 82.2 99.1 89.6 99.8 107.0 101.3 82.0 91.0 Excluded by Method 2.92 1 0.0007767 0.003154 False High Sequest HT (A2) Unambiguous EEEEDVDDVESVQDLEVK [K].eEEEDVDDVEsVQDLEVk.[L] N-Term(TMT6plex); S11(Phospho); K18(TMT6plex) 1 P36159 P36159 0 3 0.2749 0 1 1 882.07847 2644.22085 2644.21665 1.59 0.0014 478145.15625 CID (Collision Induced Dissociation) MS2 6.459174 50 20.8 60.0 99.1997 32409 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 17.8 10.0 23.5 14.8 24.2 37.7 10.2 14.9 34.8 19.6 Excluded by Method 3.71 1 0.0007767 0.004217 False High Sequest HT (A2) Unambiguous GILAADESTGSIAK [K].gILAADEsTGSIAk.[R] N-Term(TMT6plex); S8(Phospho); K14(TMT6plex) 2 P04075 P04075; P04075-2 0 2 0.0653 0 1 1 935.99929 1870.99131 1870.99273 -0.76 -0.00071 793666.8125 CID (Collision Induced Dissociation) MS2 21.94233 40 25.3 60.0 93.0604 30101 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 23.9 26.0 18.2 18.9 26.1 30.3 24.6 27.4 26.4 30.7 Excluded by Method 2.91 1 0.0007767 0.00269 False High Sequest HT (A2) Unambiguous EQSEVSVSPRASK [K].eQSEVSVsPRASk.[H] N-Term(TMT6plex); S8(Phospho); K13(TMT6plex) 1 Q7L4I2 Q7L4I2 1 3 0.1329 0 1 1 648.00627 1942.00427 1942.00469 -0.22 -0.00014 8955694.0 CID (Collision Induced Dissociation) MS2 0.0 30 113.6 20.861 51.9487 14729 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 110.2 119.2 89.0 112.4 110.7 114.6 133.0 128.4 102.9 115.7 Excluded by Method 3.01 1 0.0007767 0.002938 False High Sequest HT (A2) Unambiguous IAISPLKSPNK [K].iAIsPLksPNk.[A] N-Term(TMT6plex); S4(Phospho); K7(TMT6plex); S8(Phospho); K11(TMT6plex) 2 Q6MZP7 Q6MZP7-3; Q6MZP7 1 3 0.4184 0 1 1 672.38197 2015.13136 2015.13104 0.16 0.00011 1896889.25 CID (Collision Induced Dissociation) MS2 13.41491 10 194.5 60.0 97.9568 31945 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 196.6 196.0 139.8 241.2 190.0 176.6 241.4 200.8 180.9 181.8 Excluded by Method 2.82 1 0.0007767 0.002886 False High Sequest HT (A2) Unambiguous YNLDASEEEDSNK [K].yNLDAsEEEDSNk.[K] N-Term(TMT6plex); S6(Phospho); K13(TMT6plex) 2 O95218 O95218-2; O95218 0 2 0.4067 0 1 1 1026.46339 2051.91951 2051.92108 -0.76 -0.00078 1088780.5 CID (Collision Induced Dissociation) MS2 8.76765 30 40.3 60.0 71.0115 21865 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 38.2 31.1 39.9 42.1 38.3 41.6 40.2 40.6 49.6 41.3 Excluded by Method 3.0 1 0.0007767 0.003337 False High Sequest HT (A2) Unambiguous ALVLELDEMSNGSEIQDALEEISGQK [K].aLVLELDEmsNGSEIQDALEEISGQk.[T] N-Term(TMT6plex); M9(Oxidation); S10(Phospho); K26(TMT6plex) 2 P17695 P17695; P17695-2 0 4 0.1063 0 1 1 843.92251 3372.66823 3372.65337 4.41 0.00371 427064.5 CID (Collision Induced Dissociation) MS2 26.21079 40 37.7 60.0 144.3769 49275 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 21.4 32.8 52.7 16.6 31.3 63.4 22.2 39.3 60.8 36.3 Excluded by Method 3.95 1 0.0007767 0.004031 False High Sequest HT (A2) Unambiguous EINFDDDF [K].eINFDDDF.[-] N-Term(TMT6plex) 1 P49723 P49723 0 2 0.6667 0 1 1 622.28762 1243.56796 1243.56801 -0.04 -3e-05 944079.25 CID (Collision Induced Dissociation) MS2 21.60423 90 183.5 60.0 112.5957 37421 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 50.6 167.5 306.6 51.3 142.8 352.4 67.1 166.0 352.0 178.5 2.49 1 0.0007767 0.00338 False High Sequest HT (A2) Unambiguous ARASPSVTCK [K].aRAsPSVTck.[S] N-Term(TMT6plex); S4(Phospho); C9(Carbamidomethyl); K10(TMT6plex) 1 O15014 O15014 1 3 0.2978 0 1 1 538.953 1614.84446 1614.8439 0.34 0.00019 617687.375 CID (Collision Induced Dissociation) MS2 6.719734 60 27.6 60.0 42.7107 11296 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 26.1 28.7 23.7 26.7 28.0 25.3 30.5 30.1 32.4 24.0 Excluded by Method 2.25 1 0.0007767 0.003186 False High Sequest HT (A2) Unambiguous SPGSAGRSQTPPGVATPPIPK [K].sPGSAGRsQTPPGVATPPIPk.[I] N-Term(TMT6plex); S8(Phospho); K21(TMT6plex) 1 Q15648 Q15648 1 4 0.045 0 1 1 635.84631 2540.36343 2540.36381 -0.15 -9e-05 265717.6875 CID (Collision Induced Dissociation) MS2 27.09159 50 41.9 60.0 80.158 25303 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 40.8 45.8 30.6 47.5 40.5 43.8 42.2 42.6 43.6 41.5 Excluded by Method 2.0 1 0.0007767 0.003009 False High Sequest HT (A2) Unambiguous AASDAIPPASPK [K].aASDAIPPAsPk.[A] N-Term(TMT6plex); S10(Phospho); K12(TMT6plex) 1 P00817 P00817 0 3 0.2292 0 1 1 554.96701 1662.88646 1662.88681 -0.21 -0.00012 3527352.0 CID (Collision Induced Dissociation) MS2 8.763203 50 213.6 41.726 64.3603 19365 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 55.2 174.3 312.1 69.9 175.9 402.5 74.2 199.9 451.5 220.1 Excluded by Method 1.92 1 0.0007767 0.003338 False High Sequest HT (A2) Unambiguous GRAEGEWEDQEALDYFSDK [K].gRAEGEWEDQEALDYFsDk.[E] N-Term(TMT6plex); S17(Phospho); K19(TMT6plex) 3 Q9NYF8 Q9NYF8-3; Q9NYF8; Q9NYF8-2 1 3 0.2549 0 1 1 928.42423 2783.25814 2783.26019 -0.74 -0.00068 195548.75 CID (Collision Induced Dissociation) MS2 0.0 50 39.6 60.0 107.7815 35626 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 35.1 44.2 34.4 43.8 37.7 44.8 40.1 37.9 36.6 40.9 Excluded by Method 3.06 1 0.0007767 0.002878 False High Sequest HT (A2) Unambiguous DLDEEGSEK [K].dLDEEGsEk.[E] N-Term(TMT6plex); S7(Phospho); K9(TMT6plex) 1 O43719 O43719 0 2 0.4883 0 1 1 780.36675 1559.72622 1559.72422 1.28 0.001 1532642.125 CID (Collision Induced Dissociation) MS2 56.49207 30 288.6 60.0 55.3491 15942 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 296.5 283.3 200.1 363.6 279.6 255.1 350.6 297.2 296.7 263.6 Excluded by Method 3.83 1 0.0007767 0.003728 False High Sequest HT (A2) Unambiguous RLSLGAQK [K].rLsLGAQk.[G] N-Term(TMT6plex); S3(Phospho); K8(TMT6plex) 2 Q9H788 Q9H788; Q9H788-2 1 3 0.4548 0 1 1 470.94586 1410.82304 1410.82342 -0.27 -0.00013 3978649.0 CID (Collision Induced Dissociation) MS2 6.406693 30 89.0 50.263 65.4859 19727 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 93.0 76.0 67.8 81.0 76.2 90.6 92.1 113.6 99.9 99.8 Excluded by Method 3.21 1 0.0007767 0.002676 False High Sequest HT (A2) Unambiguous EEPAAAGSGAASPSAAEK [K].eEPAAAGSGAAsPSAAEk.[G] N-Term(TMT6plex); S12(Phospho); K18(TMT6plex) 1 P29966 P29966 0 2 0.007 0 1 1 1070.02345 2139.03962 2139.03711 1.17 0.00125 571259.0 CID (Collision Induced Dissociation) MS2 0.0 50 10.6 60.0 55.2843 15917 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 13.8 7.1 10.0 10.1 7.7 10.2 11.8 11.3 13.0 11.4 Excluded by Method 2.86 1 0.0007767 0.003483 False High Sequest HT (A2) Unambiguous RFIQELSGSSPK [K].rFIQELSGSsPk.[R] N-Term(TMT6plex); S10(Phospho); K12(TMT6plex) 1 Q01664 Q01664 1 2 0.0327 0 1 1 944.01021 1887.01314 1887.01413 -0.53 -0.0005 610038.6875 CID (Collision Induced Dissociation) MS2 0.0 20 121.8 60.0 86.2751 27547 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 112.9 121.7 100.3 141.1 118.8 116.9 135.5 126.3 126.9 117.7 Excluded by Method 2.75 1 0.0007767 0.002857 False High Sequest HT (A2) Unambiguous AVEARSPGLQK [K].aVEARsPGLQk.[E] N-Term(TMT6plex); S6(Phospho); K11(TMT6plex) 4 Q3KQU3 Q3KQU3-3; Q3KQU3-2; Q3KQU3-4; Q3KQU3 1 3 0.495 0 1 1 565.31849 1693.94093 1693.94024 0.41 0.00023 1108791.875 CID (Collision Induced Dissociation) MS2 20.32408 50 150.1 60.0 55.6382 16052 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 152.8 139.0 131.6 145.2 143.2 165.5 164.4 155.5 154.2 149.9 Excluded by Method 3.01 1 0.0007767 0.002853 False High Sequest HT (A2) Unambiguous RIACEEEFSDSEEEGEGGRK [K].rIAcEEEFsDsEEEGEGGRk.[N] N-Term(TMT6plex); C4(Carbamidomethyl); S9(Phospho); S11(Phospho); K20(TMT6plex) 1 Q13547 Q13547 2 4 0.3761 0 1 1 733.81762 2932.24865 2932.24733 0.45 0.00033 841661.5 CID (Collision Induced Dissociation) MS2 20.5446 30 51.9 60.0 62.9199 18787 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 50.6 48.3 43.8 62.0 41.9 58.0 52.1 50.4 57.4 54.5 Excluded by Method 3.51 1 0.0007767 0.003797 False High Sequest HT (A2) Unambiguous KAEGEPQEESPLK [K].kAEGEPQEEsPLk.[S] N-Term(TMT6plex); K1(TMT6plex); S10(Phospho); K13(TMT6plex) 4 Q9NYF8 Q9NYF8-3; Q9NYF8-4; Q9NYF8; Q9NYF8-2 1 4 0.4225 0 1 1 553.04886 2209.17363 2209.17204 0.72 0.0004 3207040.75 CID (Collision Induced Dissociation) MS2 12.01959 10 284.7 47.413 59.0342 17306 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 277.4 274.0 212.4 333.4 289.7 287.8 320.0 305.0 278.6 268.9 Excluded by Method 2.84 1 0.0007767 0.004245 False High Sequest HT (A2) Unambiguous SVFGTPTLETANK [K].sVFGTPTLETANk.[N] N-Term(TMT6plex); S1(Phospho); K13(TMT6plex) 1 P49792 P49792 0 3 0.0054 0 1 1 635.00483 1902.99994 1902.99782 1.12 0.00071 1085391.5 CID (Collision Induced Dissociation) MS2 6.519333 60 120.4 60.0 99.0655 32335 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 118.1 117.3 111.3 118.2 117.1 119.9 137.3 128.3 112.2 124.1 Excluded by Method 3.71 1 0.0007767 0.002965 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVssDEEF.[-] N-Term(TMT6plex); S8(Phospho); S9(Phospho) 1 P16521 P16521 0 2 0.1473 0 1 1 925.35461 1849.70195 1849.70195 0.0 0.0 738848.125 CID (Collision Induced Dissociation) MS2 15.40074 50 59.4 60.0 113.4927 37788 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 11.7 46.2 96.6 19.7 52.8 109.6 29.4 53.9 110.8 62.9 Excluded by Method 3.53 1 0.0007767 0.0042 False High Sequest HT (A2) Unambiguous EEEEAKEESDDDMGFGLFD [K].eEEEAkEEsDDDmGFGLFD.[-] N-Term(TMT6plex); K6(TMT6plex); S9(Phospho); M13(Oxidation) 1 P05318 P05318 1 3 0.6856 0 1 1 916.05098 2746.13837 2746.13669 0.61 0.00056 2744799.0 CID (Collision Induced Dissociation) MS2 0.0 40 177.7 41.816 115.0831 38374 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 45.8 155.7 245.0 69.4 155.0 305.2 60.9 183.4 374.4 182.4 Excluded by Method 3.53 1 0.0007767 0.003627 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVssDEEF.[-] N-Term(TMT6plex); S8(Phospho); S9(Phospho) 1 P16521 P16521 0 2 0.1713 0 1 1 925.35523 1849.70317 1849.70195 0.66 0.00061 268099.65625 CID (Collision Induced Dissociation) MS2 0.0 40 45.0 60.0 140.3547 47885 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 12.9 38.8 74.7 15.9 39.0 76.3 11.3 44.6 91.8 44.6 Excluded by Method 2.86 1 0.0007767 0.002919 False High Sequest HT (A2) Unambiguous DVQSPGFLNEPLSSK [K].dVQsPGFLNEPLSSk.[S] N-Term(TMT6plex); S4(Phospho); K15(TMT6plex) 4 Q8NG31 Q8NG31-4; Q8NG31-3; Q8NG31-2; Q8NG31 0 3 0.2811 0 1 1 719.37294 2156.10426 2156.10407 0.09 6e-05 970114.9375 CID (Collision Induced Dissociation) MS2 6.48874 40 28.6 60.0 106.6855 35211 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 24.7 26.3 31.3 24.1 24.7 36.5 25.8 29.6 37.1 26.1 Excluded by Method 2.49 1 0.0007767 0.003987 False High Sequest HT (A2) Unambiguous EEEEAKEESDDDMGFGLFD [K].eEEEAkEEsDDDmGFGLFD.[-] N-Term(TMT6plex); K6(TMT6plex); S9(Phospho); M13(Oxidation) 1 P05318 P05318 1 3 0.7404 0 1 1 916.05065 2746.13739 2746.13669 0.25 0.00023 9415882.0 CID (Collision Induced Dissociation) MS2 0.8275039 50 197.4 11.613 114.2032 37979 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 43.2 157.1 297.6 63.8 170.9 353.2 66.6 192.9 427.6 200.6 Excluded by Method 3.89 1 0.0007767 0.003106 False High Sequest HT (A2) Unambiguous KDESDSENDIEIK [K].kDEsDsENDIEIk.[G] N-Term(TMT6plex); K1(TMT6plex); S4(Phospho); S6(Phospho); K13(TMT6plex) 1 P32499 P32499 1 3 0.4459 0 1 1 790.37557 2369.11216 2369.11295 -0.33 -0.00026 901783.125 CID (Collision Induced Dissociation) MS2 24.11949 50 183.1 60.0 77.5874 24342 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 73.0 163.0 254.0 86.5 170.1 274.6 95.1 186.6 344.6 183.7 Excluded by Method 4.53 1 0.0007767 0.004169 False High Sequest HT (A2) Unambiguous AFSIIPSQRFIK [K].aFSIIPsQRFIk.[S] N-Term(TMT6plex); S7(Phospho); K12(TMT6plex) 1 P38970 P38970 1 3 0.48 0 1 1 649.04088 1945.10809 1945.10764 0.23 0.00015 1460071.75 CID (Collision Induced Dissociation) MS2 4.875693 20 81.4 60.0 117.4441 39249 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 30.6 69.5 113.8 36.1 60.9 150.1 40.3 78.3 147.5 87.3 Excluded by Method 3.5 1 0.0007767 0.004074 False High Sequest HT (A2) Unambiguous IMATPEQVGK [K].iMAtPEQVGk.[M] N-Term(TMT6plex); T4(Phospho); K10(TMT6plex) 2 P12956 P12956; P12956-2 0 2 0.4694 0 1 1 806.43148 1611.85567 1611.85815 -1.54 -0.00124 661455.5 CID (Collision Induced Dissociation) MS2 0.0 20 72.8 60.0 81.9104 25913 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 81.8 63.3 61.4 62.6 69.7 75.8 78.9 82.9 70.0 82.0 Excluded by Method 3.6 1 0.0007767 0.002706 False High Sequest HT (A2) Unambiguous TQSSESRLYELSAEDLEK [K].tQSSESRLYELsAEDLEk.[E] N-Term(TMT6plex); T1(Phospho); S12(Phospho); K18(TMT6plex) 1 P53885 P53885 1 3 0.1352 0 1 1 901.75758 2703.25819 2703.25676 0.53 0.00048 1218893.875 CID (Collision Induced Dissociation) MS2 30.78296 30 28.0 60.0 103.0493 33815 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 14.9 26.6 33.3 16.5 23.9 45.6 17.0 25.9 51.6 25.0 Excluded by Method 3.18 1 0.0007767 0.004229 False High Sequest HT (A2) Unambiguous SNNEVTEHSDSEDLTEK [K].sNNEVTEHsDSEDLTEk.[Q] N-Term(TMT6plex); S9(Phospho); K17(TMT6plex) 1 P40563 P40563 0 3 0.2326 0 1 1 824.71136 2472.11952 2472.11794 0.64 0.00053 767785.0625 CID (Collision Induced Dissociation) MS2 0.0 60 9.4 60.0 56.3694 16347 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 3.8 6.6 12.3 6.5 9.0 14.2 6.3 11.1 17.7 6.1 Excluded by Method 2.58 1 0.0007767 0.003321 False High Sequest HT (A2) Unambiguous SFDDTIELK [K].sFDDTIELk.[K] N-Term(TMT6plex); S1(Phospho); K9(TMT6plex) 1 P39104 P39104 0 3 0.1354 0 1 1 535.94414 1605.81786 1605.81773 0.08 4e-05 2266311.0 CID (Collision Induced Dissociation) MS2 6.549214 60 116.8 60.0 104.9635 34621 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 32.1 86.5 161.3 40.3 95.7 231.7 43.3 105.4 248.6 123.4 Excluded by Method 3.25 1 0.0007767 0.003846 False High Sequest HT (A2) Unambiguous VGSTENIK [K].vGsTENIk.[H] N-Term(TMT6plex); S3(Phospho); K8(TMT6plex) 4 P27816 P27816; P27816-5; P27816-6; P27816-2 0 2 0.1041 0 1 1 693.37551 1385.74375 1385.74417 -0.3 -0.00021 2579298.5 CID (Collision Induced Dissociation) MS2 11.4972 20 88.6 60.0 62.9179 18766 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 85.4 89.9 61.6 110.5 85.1 85.0 103.0 96.4 84.2 84.4 Excluded by Method 3.17 1 0.0007767 0.003475 False High Sequest HT (A2) Unambiguous ISLSPSTESLADSK [K].iSLSPsTEsLADSk.[S] N-Term(TMT6plex); S6(Phospho); S9(Phospho); K14(TMT6plex) 1 P31374 P31374 0 2 0.2615 0 1 1 1026.99953 2052.99178 2052.99076 0.5 0.00051 504538.0625 CID (Collision Induced Dissociation) MS2 0.0 30 69.3 60.0 106.668 35249 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 19.9 58.0 104.8 24.9 58.5 125.5 29.3 62.2 142.2 68.2 Excluded by Method 3.48 1 0.0007767 0.003439 False High Sequest HT (A2) Unambiguous SYQNSPSSDDGIRPLPEYSTEK [K].sYQNSPssDDGIRPLPEYSTEk.[H] N-Term(TMT6plex); S7(Phospho); S8(Phospho); K22(TMT6plex) 1 Q15648 Q15648 0 3 0.0636 0 1 1 1030.13685 3088.39601 3088.39538 0.2 0.00021 648404.625 CID (Collision Induced Dissociation) MS2 0.0 30 53.4 60.0 91.689 29640 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 47.1 45.6 56.8 49.1 58.3 56.0 58.7 59.6 55.7 47.6 Excluded by Method 3.93 1 0.0007767 0.003556 False High Sequest HT (A2) Unambiguous CSLPAEEDSVLEK [K].cSLPAEEDsVLEk.[L] N-Term(TMT6plex); C1(Carbamidomethyl); S9(Phospho); K13(TMT6plex) 2 P27816 P27816; P27816-6 0 3 0.3471 0 1 1 672.3312 2014.97905 2014.98085 -0.89 -0.0006 1032457.3125 CID (Collision Induced Dissociation) MS2 42.34048 50 55.8 60.0 91.6743 29634 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 47.6 52.8 71.0 39.7 44.8 86.6 43.3 46.3 72.3 53.5 Excluded by Method 3.4 1 0.0007767 0.003022 False High Sequest HT (A2) Unambiguous IPSLGWSPSK [K].iPSLGWsPSk.[H] N-Term(TMT6plex); S7(Phospho); K10(TMT6plex) 2 Q8IVF2 Q8IVF2-3; Q8IVF2 0 3 0.3805 0 1 1 537.29701 1609.87648 1609.87552 0.6 0.00032 429992.3125 CID (Collision Induced Dissociation) MS2 18.04139 40 136.4 60.0 115.9946 38669 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 139.0 129.4 114.4 138.6 136.0 133.1 161.8 157.4 125.0 128.8 Excluded by Method 4.1 1 0.0007767 0.003033 False High Sequest HT (A2) Unambiguous SVNYNELSDDDTAVK [K].sVNYNELsDDDTAVk.[N] N-Term(TMT6plex); S8(Phospho); K15(TMT6plex) 1 P31244 P31244 0 3 0.131 0 1 1 736.68755 2208.0481 2208.04734 0.34 0.00025 1707477.375 CID (Collision Induced Dissociation) MS2 2.790735 70 56.8 60.0 81.5286 25769 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 23.2 50.3 84.0 23.5 47.1 108.4 26.5 54.5 95.0 55.1 2.52 1 0.0007767 0.003047 False High Sequest HT (A2) Unambiguous SGLELTELQNMTVPEDDNISNDSNDFTEVENGQINSK [K].sGLELTELQNmTVPEDDNIsNDsNDFTEVENGQINSk.[F] N-Term(TMT6plex); M11(Oxidation); S20(Phospho); S23(Phospho); K37(TMT6plex) 1 Q9H2H9 Q9H2H9 0 4 0.2228 0 1 1 1183.5291 4731.09459 4731.09459 0.0 0.0 277479.03125 CID (Collision Induced Dissociation) MS2 0.0 60 6.4 60.0 117.3304 39123 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 4.2 6.3 8.6 3.8 4.0 9.1 8.1 8.8 7.3 3.7 Excluded by Method 3.86 1 0.0007767 0.002896 False High Sequest HT (A2) Unambiguous QTGERSIFSQEVVELPDSEDETQQVNK [K].qTGERSIFSQEVVELPDsEDETQQVNk.[T] N-Term(TMT6plex); S18(Phospho); K27(TMT6plex) 1 P53885 P53885 1 4 0.0243 0 1 1 908.44625 3630.76316 3630.75765 1.52 0.00138 941503.0625 CID (Collision Induced Dissociation) MS2 0.0 60 35.3 60.0 104.7555 34545 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 7.0 28.8 55.9 10.3 26.8 70.3 17.0 28.8 69.0 39.5 Excluded by Method 3.71 1 0.0007767 0.003135 False High Sequest HT (A2) Unambiguous ATVNARPQRVLDTSSLTQSAPASPTNK [K].aTVNARPQRVLDTSSLTQSAPAsPTNk.[G] N-Term(TMT6plex); S23(Phospho); K27(TMT6plex) 2 Q8N122 Q8N122-3; Q8N122 1 4 0.1658 0 1 1 837.94657 3348.76443 3348.7677 -0.98 -0.00082 884245.0625 CID (Collision Induced Dissociation) MS2 0.0 30 40.3 60.0 75.3749 23504 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 39.9 36.3 30.1 38.6 41.5 41.3 42.2 47.4 43.8 42.2 Excluded by Method 3.86 1 0.0007767 0.004148 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVssDEEF.[-] N-Term(TMT6plex); S8(Phospho); S9(Phospho) 1 P16521 P16521 0 2 0.1306 0 1 1 925.35471 1849.70214 1849.70195 0.1 9e-05 1435897.625 CID (Collision Induced Dissociation) MS2 12.96892 30 118.5 59.754 114.4731 38080 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 25.9 87.6 193.0 31.8 100.2 219.4 33.3 116.7 247.7 129.8 Excluded by Method 3.37 1 0.0007767 0.003832 False High Sequest HT (A2) Unambiguous NFHAEVSTPQVLSAK [K].nFHAEVsTPQVLSAk.[K] N-Term(TMT6plex); S7(Phospho); K15(TMT6plex) 1 P48570 P48570 0 3 0.2926 0 1 1 722.71811 2166.13978 2166.13604 1.72 0.00125 989327.125 CID (Collision Induced Dissociation) MS2 47.90998 40 51.4 60.0 88.1199 28299 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 36.5 39.2 62.1 38.6 44.7 77.2 39.2 53.4 73.0 50.6 Excluded by Method 3.11 1 0.0007767 0.004042 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVsSDEEF.[-] N-Term(TMT6plex); S8(Phospho) 1 P16521 P16521 0 2 0.0417 0 1 1 885.36906 1769.73085 1769.73562 -2.7 -0.00239 343647.625 CID (Collision Induced Dissociation) MS2 32.70276 40 43.5 60.0 137.0216 46738 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 11.6 31.1 76.0 12.7 35.5 72.7 14.8 42.1 90.8 48.1 Excluded by Method 3.12 1 0.0007767 0.004306 False High Sequest HT (A2) Unambiguous NAEEESESEAEEGD [K].nAEEEsEsEAEEGD.[-] N-Term(TMT6plex); S6(Phospho); S8(Phospho) 4 Q7L1Q6 Q7L1Q6-2; Q7L1Q6-4; Q7L1Q6-3; Q7L1Q6 0 2 0.9761 0 1 1 957.32406 1913.64085 1913.6412 -0.18 -0.00018 203561.4375 CID (Collision Induced Dissociation) MS2 0.0 40 7.9 60.0 42.2568 11174 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 4.0 9.5 7.0 11.5 7.9 7.7 8.7 7.7 8.8 6.1 Excluded by Method 2.51 1 0.0007767 0.002872 False High Sequest HT (A2) Unambiguous AVLFCLSEDK [K].aVLFcLSEDk.[K] N-Term(TMT6plex); C5(Carbamidomethyl); K10(TMT6plex) 1 P23528 P23528 0 3 0.3224 0 1 1 547.30951 1639.91397 1639.91295 0.62 0.00034 653063.875 CID (Collision Induced Dissociation) MS2 30.88105 90 42.9 60.0 109.9169 36419 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 26.2 46.4 39.5 37.0 37.4 51.5 34.9 45.0 58.3 53.0 2.14 1 0.0007767 0.003521 False High Sequest HT (A2) Unambiguous SFDDTIELK [K].sFDDTIELk.[K] N-Term(TMT6plex); S1(Phospho); K9(TMT6plex) 1 P39104 P39104 0 2 0.3362 0 1 1 803.41334 1605.8194 1605.81773 1.04 0.00084 2689169.5 CID (Collision Induced Dissociation) MS2 72.68829 40 72.0 29.367 105.1623 34608 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 21.4 52.2 105.3 25.2 54.8 142.2 31.2 71.5 143.1 72.7 Excluded by Method 3.45 1 0.0007767 0.003057 False High Sequest HT (A2) Unambiguous GRAEGEWEDQEALDYFSDK [K].gRAEGEWEDQEALDYFsDk.[E] N-Term(TMT6plex); S17(Phospho); K19(TMT6plex) 3 Q9NYF8 Q9NYF8-3; Q9NYF8; Q9NYF8-2 1 3 0.1638 0 1 1 928.42521 2783.26107 2783.26019 0.32 0.00029 591418.125 CID (Collision Induced Dissociation) MS2 29.00065 10 52.0 60.0 105.2741 34691 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 49.9 57.6 45.3 45.1 47.7 51.6 61.1 53.2 56.6 52.0 Excluded by Method 3.54 1 0.0007767 0.003085 False High Sequest HT (A2) Unambiguous EQSEVSVSPRASK [K].eQSEVSVSPRAsk.[H] N-Term(TMT6plex); S12(Phospho); K13(TMT6plex) 1 Q7L4I2 Q7L4I2 1 4 0.0465 0 1 1 486.25696 1942.00602 1942.00469 0.68 0.00033 1345236.5 CID (Collision Induced Dissociation) MS2 8.708235 30 38.2 60.0 52.3645 14832 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 35.0 41.2 31.7 35.5 34.8 35.1 42.0 41.0 37.5 48.4 Excluded by Method 3.01 1 0.0007767 0.004237 False High Sequest HT (A2) Unambiguous SAEGNNIDQSLILK [K].sAEGNNIDQSLILk.[E] N-Term(TMT6plex); S1(Phospho); K14(TMT6plex) 1 P39705 P39705 0 3 0.1923 0 1 1 680.69768 2040.07849 2040.07786 0.31 0.00021 821898.75 CID (Collision Induced Dissociation) MS2 9.869368 40 84.1 60.0 104.9298 34521 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 23.2 61.9 128.7 31.6 74.3 157.0 33.3 75.4 162.3 93.5 Excluded by Method 2.34 1 0.0007767 0.002641 False High Sequest HT (A2) Unambiguous EMLASDDEEDVSSK [K].eMLAsDDEEDVSSk.[V] N-Term(TMT6plex); S5(Phospho); K14(TMT6plex) 4 Q0ZGT2 Q0ZGT2-2; Q0ZGT2-4; Q0ZGT2; Q0ZGT2-3 0 3 0.6146 0 1 1 698.31752 2092.93801 2092.93977 -0.84 -0.00059 4314826.0 CID (Collision Induced Dissociation) MS2 4.335625 60 61.2 28.925 78.4344 24573 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 57.3 57.2 47.5 67.6 58.1 59.6 60.7 66.4 67.6 70.5 Excluded by Method 3.01 1 0.0007767 0.003441 False High Sequest HT (A2) Unambiguous DLDEEGSEK [K].dLDEEGsEk.[E] N-Term(TMT6plex); S7(Phospho); K9(TMT6plex) 1 O43719 O43719 0 2 0.4746 0 1 1 780.36651 1559.72574 1559.72422 0.97 0.00076 7391308.0 CID (Collision Induced Dissociation) MS2 15.42241 40 235.7 56.323 54.6084 15697 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 247.9 234.2 171.0 278.3 231.3 225.4 269.6 233.4 230.9 235.2 Excluded by Method 3.94 1 0.0007767 0.003439 False High Sequest HT (A2) Unambiguous DVPPLSETEATPVPIK [K].dVPPLSETEAtPVPIk.[D] N-Term(TMT6plex); T11(Phospho); K16(TMT6plex) 2 P27816 P27816; P27816-6 0 3 0.2853 0 1 1 744.40447 2231.19887 2231.19764 0.55 0.00041 1293627.625 CID (Collision Induced Dissociation) MS2 16.18527 20 278.0 57.015 104.8101 34476 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 289.3 275.0 238.6 305.9 258.5 258.7 307.0 292.1 285.8 268.7 Excluded by Method 3.19 1 0.0007767 0.002925 False High Sequest HT (A2) Unambiguous IGIVEISPK [K].iGIVEIsPk.[G] N-Term(TMT6plex); S7(Phospho); K9(TMT6plex) 2 P07280 P07280; P07281 0 3 0.6369 0 1 1 498.62982 1493.87489 1493.87445 0.29 0.00015 428320.09375 CID (Collision Induced Dissociation) MS2 40.11005 70 22.1 60.0 107.8162 35671 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 3.0 16.9 33.7 9.4 17.4 43.0 10.8 20.3 45.0 21.5 3.14 1 0.0007767 0.003294 False High Sequest HT (A2) Unambiguous RLLGDSDSEEEQK [K].rLLGDsDsEEEQk.[E] N-Term(TMT6plex); S6(Phospho); S8(Phospho); K13(TMT6plex) 1 Q9BW71 Q9BW71 1 3 0.4748 0 1 1 708.66037 2123.96655 2123.96633 0.1 7e-05 484104.875 CID (Collision Induced Dissociation) MS2 40.24799 20 60.9 60.0 77.2714 24172 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 44.3 58.5 53.3 50.9 63.7 75.5 55.6 71.5 68.5 67.2 Excluded by Method 3.37 1 0.0007767 0.004166 False High Sequest HT (A2) Unambiguous DLSSLYSEK [K].dLSSLYsEk.[K] N-Term(TMT6plex); S7(Phospho); K9(TMT6plex) 1 P25558 P25558 0 2 0.1182 0 1 1 790.40476 1579.80224 1579.80208 0.1 8e-05 3889762.0 CID (Collision Induced Dissociation) MS2 24.92965 0 54.9 38.774 97.7623 31840 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 26.0 47.5 67.3 20.4 49.7 103.3 26.8 53.9 96.9 57.2 Excluded by Method 3.3 1 0.0007767 0.004296 False High Sequest HT (A2) Unambiguous RIACEEEFSDSEEEGEGGRK [K].rIAcEEEFsDsEEEGEGGRk.[N] N-Term(TMT6plex); C4(Carbamidomethyl); S9(Phospho); S11(Phospho); K20(TMT6plex) 1 Q13547 Q13547 2 3 0.5955 0 1 1 978.08832 2932.25042 2932.24733 1.05 0.00103 231558.4375 CID (Collision Induced Dissociation) MS2 0.0 20 84.7 60.0 61.9945 18473 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 91.4 93.6 68.9 84.7 77.1 74.9 101.7 89.4 83.9 81.8 Excluded by Method 3.09 1 0.0007767 0.004037 False High Sequest HT (A2) Unambiguous KEESEESDDDMGFGLFD [K].kEESEEsDDDmGFGLFD.[-] N-Term(TMT6plex); K1(TMT6plex); S7(Phospho); M11(Oxidation) 3 P05387 P05386; P05386-2; P05387 1 2 0.0329 0 1 1 1252.52651 2504.04575 2504.04642 -0.27 -0.00034 930018.875 CID (Collision Induced Dissociation) MS2 0.0 40 76.8 60.0 113.0795 37635 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 79.4 77.4 60.4 72.5 70.1 73.2 83.1 89.1 82.8 80.2 Excluded by Method 3.04 1 0.0007767 0.003034 False High Sequest HT (A2) Unambiguous QEAGDSPPPAPGTPK [K].qEAGDsPPPAPGTPk.[A] N-Term(TMT6plex); S6(Phospho); K15(TMT6plex) 4 P49848 P49848-3; P49848; P49848-4; P49848-2 0 3 0.1624 0 1 1 663.00272 1986.9936 1986.99379 -0.1 -6e-05 649434.9375 CID (Collision Induced Dissociation) MS2 20.26909 60 25.2 60.0 55.8531 16184 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 24.5 25.4 22.7 21.2 27.2 24.3 24.3 26.1 27.3 28.8 Excluded by Method 3.14 1 0.0007767 0.002762 False High Sequest HT (A2) Unambiguous AYSPTCSPTLGFK [K].aYsPTcsPTLGFk.[E] N-Term(TMT6plex); S3(Phospho); C6(Carbamidomethyl); S7(Phospho); K13(TMT6plex) 1 Q5TGY3 Q5TGY3 0 3 0.0665 0 1 1 682.98341 2046.93568 2046.9413 -2.75 -0.00187 1032370.125 CID (Collision Induced Dissociation) MS2 0.0 50 121.4 60.0 108.1958 35808 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 121.9 127.0 111.5 120.0 110.0 113.9 144.4 129.1 118.9 117.3 Excluded by Method 4.06 1 0.0007767 0.003767 False High Sequest HT (A2) Unambiguous SASPVLPGVK [K].sAsPVLPGVk.[R] N-Term(TMT6plex); S3(Phospho); K10(TMT6plex) 1 P38272 P38272 0 2 0.251 0 1 1 746.93085 1492.85443 1492.85405 0.25 0.00019 974987.0625 CID (Collision Induced Dissociation) MS2 58.02121 30 58.9 60.0 85.2891 27236 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 25.3 55.9 81.4 30.2 49.1 103.9 22.5 53.6 103.1 63.9 Excluded by Method 2.43 1 0.0007767 0.00305 False High Sequest HT (A2) Unambiguous FYDDENLTE [K].fYDDENLtE.[-] N-Term(TMT6plex); T8(Phospho) 1 P50278 P50278 0 2 0.6779 0 1 1 727.8002 1454.59312 1454.59259 0.36 0.00026 45487852.0 CID (Collision Induced Dissociation) MS2 29.64562 30 128.0 2.986 94.9798 30872 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 66.2 102.5 167.1 66.1 115.0 216.3 72.5 120.3 224.4 129.1 Excluded by Method 2.67 1 0.0007767 0.003987 False High Sequest HT (A2) Unambiguous LFESDEEETNGENIQINK [K].lFEsDEEETNGENIQINk.[G] N-Term(TMT6plex); S4(Phospho); K18(TMT6plex) 1 Q04500 Q04500 0 3 0.1261 0 1 1 883.08946 2647.25383 2647.25404 -0.08 -7e-05 542236.375 CID (Collision Induced Dissociation) MS2 9.997229 60 31.2 60.0 107.6972 35626 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 4.3 22.7 39.0 15.3 31.8 57.7 14.4 30.7 61.8 33.8 Excluded by Method 2.38 1 0.0007767 0.002702 False High Sequest HT (A2) Unambiguous APVPSTCSSTFPEELSPPSHQAK [K].aPVPSTcSSTFPEELsPPSHQAk.[R] N-Term(TMT6plex); C7(Carbamidomethyl); S16(Phospho); K23(TMT6plex) 5 Q14980 Q14980-3; Q14980-5; Q14980-2; Q14980-4; Q14980 0 4 0.0273 0 1 1 748.86957 2992.45646 2992.45276 1.24 0.00092 1131894.25 CID (Collision Induced Dissociation) MS2 2.454783 10 144.7 60.0 89.3258 28750 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 152.3 147.2 133.9 147.6 139.9 150.7 160.3 148.2 135.0 131.5 Excluded by Method 4.39 1 0.0007767 0.003544 False High Sequest HT (A2) Unambiguous TASQIALNESAK [K].tASQIALNESAk.[S] N-Term(TMT6plex); T1(Phospho); K12(TMT6plex) 1 P38742 P38742 0 3 0.043 0 1 1 590.98474 1770.93968 1770.9403 -0.35 -0.00021 923834.125 CID (Collision Induced Dissociation) MS2 3.573396 50 43.7 60.0 75.9273 23659 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 20.6 44.3 52.5 19.6 40.3 66.7 23.9 40.9 79.0 49.5 Excluded by Method 2.79 1 0.0007767 0.003124 False High Sequest HT (A2) Unambiguous QSSLAEPVSPSK [K].qSSLAEPVsPSk.[K] N-Term(TMT6plex); S9(Phospho); K12(TMT6plex) 1 O43572 O43572 0 3 0.0224 0 1 1 589.98117 1767.92896 1767.9294 -0.25 -0.00015 1744584.5 CID (Collision Induced Dissociation) MS2 0.0 40 84.3 60.0 66.8151 20215 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 72.3 86.1 69.1 87.6 84.6 88.3 87.1 88.2 93.3 86.3 Excluded by Method 3.13 1 0.0007767 0.002838 False High Sequest HT (A2) Unambiguous QEAGDSPPPAPGTPK [K].qEAGDsPPPAPGtPk.[A] N-Term(TMT6plex); S6(Phospho); T13(Phospho); K15(TMT6plex) 4 P49848 P49848-3; P49848; P49848-4; P49848-2 0 3 0.4519 0 1 1 689.65838 2066.96058 2066.96012 0.22 0.00015 478902.40625 CID (Collision Induced Dissociation) MS2 60.07111 60 42.2 60.0 55.4263 15999 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 37.1 39.5 37.8 43.4 37.9 40.0 40.7 50.0 50.9 44.3 Excluded by Method 3.43 1 0.0007767 0.004149 False High Sequest HT (A2) Unambiguous EVDPSTGELQSLQMPESEGPSSLDPSQEGPTGLK [K].eVDPSTGELQSLQmPESEGPsSLDPSQEGPTGLk.[E] N-Term(TMT6plex); M14(Oxidation); S21(Phospho); K34(TMT6plex) 2 Q13501 Q13501-2; Q13501 0 4 0.0171 0 1 1 1020.98526 4080.91923 4080.92485 -1.38 -0.00141 270857.6875 CID (Collision Induced Dissociation) MS2 0.0 30 13.9 60.0 106.3101 35073 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 14.7 15.9 15.5 15.6 11.0 13.2 15.3 10.9 12.9 13.8 Excluded by Method 3.51 1 0.0007767 0.003458 False High Sequest HT (A2) Unambiguous LENESLPNLSG [K].lENESLPNLsG.[-] N-Term(TMT6plex); S10(Phospho) 1 Q05024 Q05024 0 2 0.3919 0 1 1 741.35829 1481.7093 1481.70862 0.46 0.00034 2086794.75 CID (Collision Induced Dissociation) MS2 15.71811 60 112.8 57.717 103.6957 34149 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 33.5 85.8 186.0 36.5 88.2 220.4 42.3 101.8 219.2 114.5 Excluded by Method 2.22 1 0.0007767 0.003007 False High Sequest HT (A2) Unambiguous GSEDSPPK [K].gSEDsPPk.[H] N-Term(TMT6plex); S5(Phospho); K8(TMT6plex) 3 P48651 P48651; P48651-3; P48651-2 0 2 0.4281 0 1 1 677.83631 1354.66534 1354.66558 -0.18 -0.00012 913092.9375 CID (Collision Induced Dissociation) MS2 3.361737 50 254.3 60.0 39.9015 10337 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 255.4 244.3 201.9 294.5 238.1 229.7 291.5 277.0 257.4 253.3 Excluded by Method 3.2 1 0.0007767 0.002835 False High Sequest HT (A2) Unambiguous YLEIESSPLK [K].yLEIESsPLk.[L] N-Term(TMT6plex); S7(Phospho); K10(TMT6plex) 1 P13382 P13382 0 3 0.1674 0 1 1 572.97879 1716.9218 1716.92253 -0.42 -0.00024 1496437.125 CID (Collision Induced Dissociation) MS2 3.408439 50 20.6 60.0 106.5369 35156 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 11.8 19.7 25.3 10.2 20.3 27.7 10.3 22.0 35.2 23.6 Excluded by Method 2.27 1 0.0007767 0.003189 False High Sequest HT (A2) Unambiguous TPPRWAEDQNSLLK [K].tPPRWAEDQNSLLk.[M] N-Term(TMT6plex); T1(Phospho); K14(TMT6plex) 3 P49792 P49792; A6NKT7; Q7Z3J3 1 3 0.5149 0 1 1 731.72054 2193.14707 2193.14694 0.06 4e-05 655631.125 CID (Collision Induced Dissociation) MS2 0.0 40 81.1 60.0 95.5244 31081 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 89.1 70.6 76.7 88.9 81.3 77.9 87.5 73.5 82.5 82.8 Excluded by Method 3.03 1 0.0007767 0.004324 False High Sequest HT (A2) Unambiguous EALDQSF [K].eALDQsF.[-] N-Term(TMT6plex); S6(Phospho) 1 Q9HAS0 Q9HAS0 0 2 0.6522 0 1 1 559.75179 1118.4963 1118.49684 -0.48 -0.00027 1172891.0 CID (Collision Induced Dissociation) MS2 0.0 40 59.2 60.0 85.1358 27178 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 54.1 62.0 51.5 55.0 61.1 62.9 56.8 65.3 60.6 62.2 Excluded by Method 2.07 1 0.0007767 0.003509 False High Sequest HT (A2) Unambiguous LAVGDNNSK [K].lAVGDNNSk.[W] N-Term(TMT6plex); K9(TMT6plex) 1 P46940 P46940 0 2 0.4642 0 1 1 688.40064 1375.79401 1375.79455 -0.4 -0.00027 647749.1875 CID (Collision Induced Dissociation) MS2 18.95638 60 31.2 60.0 55.6154 16097 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 27.1 32.8 22.1 31.2 32.9 26.8 39.9 32.3 32.3 35.0 Excluded by Method 2.65 1 0.0007767 0.004081 False High Sequest HT (A2) Unambiguous QEAGDSPPPAPGTPK [K].qEAGDsPPPAPGtPk.[A] N-Term(TMT6plex); S6(Phospho); T13(Phospho); K15(TMT6plex) 4 P49848 P49848-3; P49848; P49848-4; P49848-2 0 3 0.4726 0 1 1 689.65809 2066.95971 2066.96012 -0.2 -0.00014 434436.28125 CID (Collision Induced Dissociation) MS2 48.49475 50 34.9 60.0 56.0935 16273 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 39.9 36.5 30.4 39.8 29.3 31.4 36.3 35.3 33.1 36.8 Excluded by Method 3.28 1 0.0007767 0.003004 False High Sequest HT (A2) Unambiguous TSPENVQDRFALVTPK [K].tsPENVQDRFALVTPk.[K] N-Term(TMT6plex); S2(Phospho); K16(TMT6plex) 1 P49792 P49792 1 4 0.0069 0 1 1 585.81492 2340.23784 2340.23648 0.58 0.00034 1006253.25 CID (Collision Induced Dissociation) MS2 0.0 40 109.3 60.0 96.4153 31328 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 110.3 110.5 95.2 107.9 113.5 109.0 113.4 116.4 116.2 100.7 Excluded by Method 2.9 1 0.0007767 0.003308 False High Sequest HT (A2) Unambiguous TSELFPLSGSESGDIK [K].tSELFPLsGSESGDIk.[A] N-Term(TMT6plex); S8(Phospho); K16(TMT6plex) 1 P40563 P40563 0 3 0.042 0 1 1 735.70759 2205.10822 2205.10922 -0.45 -0.00033 570069.9375 CID (Collision Induced Dissociation) MS2 0.0 60 38.8 60.0 115.867 38589 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 15.4 29.5 54.3 15.4 31.3 68.3 13.9 38.4 77.7 43.5 Excluded by Method 2.62 1 0.0007767 0.004173 False High Sequest HT (A2) Unambiguous DDSHSAEDSEDEK [K].dDSHsAEDsEDEk.[E] N-Term(TMT6plex); S5(Phospho); S9(Phospho); K13(TMT6plex) 2 Q9H1E3 Q9H1E3; Q9H1E3-2 0 3 0.5037 0 1 1 694.60484 2081.79998 2081.79899 0.47 0.00033 167420.25 CID (Collision Induced Dissociation) MS2 53.53394 40 27.5 60.0 33.85 8166 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 31.0 28.1 20.8 32.1 23.1 23.1 27.0 28.5 28.1 32.8 Excluded by Method 2.7 1 0.0009597 0.004436 False High Sequest HT (A2) Unambiguous EEEEDVDDVESVQDLEVK [K].eEEEDVDDVEsVQDLEVk.[L] N-Term(TMT6plex); S11(Phospho); K18(TMT6plex) 1 P36159 P36159 0 3 0.0978 0 1 1 882.07646 2644.21484 2644.21665 -0.69 -0.0006 554926.0 CID (Collision Induced Dissociation) MS2 0.0 50 29.5 60.0 100.0106 32686 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 21.1 19.6 26.6 29.0 34.6 46.4 19.2 24.1 49.0 25.1 Excluded by Method 2.76 1 0.0009597 0.004449 False High Sequest HT (A2) Unambiguous ASAPERTPSPAPK [K].aSAPERTPsPAPk.[R] N-Term(TMT6plex); S9(Phospho); K13(TMT6plex) 4 Q63ZY6 Q63ZY6-6; Q63ZY6; Q96P11-2; Q3KNT7-3 1 3 0.2054 0 1 1 616.33327 1846.98527 1846.98283 1.32 0.00081 288295.1875 CID (Collision Induced Dissociation) MS2 42.38916 40 288.6 60.0 45.5307 12363 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 287.4 296.4 232.6 297.5 310.9 279.8 321.2 314.2 261.4 285.0 Excluded by Method 3.36 1 0.0009597 0.00453 False High Sequest HT (A2) Unambiguous EEDEEPESPPEK [K].eEDEEPEsPPEk.[K] N-Term(TMT6plex); S8(Phospho); K12(TMT6plex) 2 Q9H1E3 Q9H1E3; Q9H1E3-2 0 3 0.567 0 1 1 651.63072 1952.8776 1952.87782 -0.11 -7e-05 4575660.5 CID (Collision Induced Dissociation) MS2 9.07233 50 209.0 22.825 53.702 15358 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 209.7 216.6 171.9 230.5 205.7 184.9 244.2 217.8 206.2 202.3 Excluded by Method 2.91 1 0.0009597 0.004516 False High Sequest HT (A2) Unambiguous SFDDTIELK [K].sFDDTIELk.[K] N-Term(TMT6plex); S1(Phospho); K9(TMT6plex) 1 P39104 P39104 0 3 0.1375 0 1 1 535.94374 1605.81667 1605.81773 -0.66 -0.00035 1911282.375 CID (Collision Induced Dissociation) MS2 4.731788 60 121.7 60.0 104.2824 34320 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 30.7 111.0 180.9 38.6 97.5 232.3 46.6 113.0 253.9 113.0 Excluded by Method 3.49 1 0.0009597 0.00452 False High Sequest HT (A2) Unambiguous LGDVSPTQIDVSQFGSFK [K].lGDVsPTQIDVSQFGSFk.[E] N-Term(TMT6plex); S5(Phospho); K18(TMT6plex) 1 P46821 P46821 0 3 0.0556 0 1 1 821.75748 2463.25789 2463.25728 0.25 0.00021 135959264.0 CID (Collision Induced Dissociation) MS2 1.20055 50 41.0 0.905 128.3888 43322 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 42.8 31.3 42.6 42.1 39.2 37.1 47.0 45.9 41.8 39.9 Excluded by Method 2.34 1 0.0009597 0.004385 False High Sequest HT (A2) Unambiguous FLNIENPENQSE [K].fLNIENPENQsE.[-] N-Term(TMT6plex); S11(Phospho) 1 Q04779 Q04779 0 3 0.3172 0 1 1 581.5993 1742.78335 1742.78358 -0.13 -8e-05 319207.09375 CID (Collision Induced Dissociation) MS2 0.0 40 55.5 60.0 118.6452 39700 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 28.0 46.5 64.0 22.4 50.2 92.6 40.7 62.3 86.7 61.2 Excluded by Method 3.72 1 0.0009597 0.004523 False High Sequest HT (A2) Unambiguous TFNSPNLK [K].tFNsPNLk.[D] N-Term(TMT6plex); S4(Phospho); K8(TMT6plex) 5 Q9Y6N7 Q9Y6N7; Q9Y6N7-4; Q9Y6N7-5; Q9Y6N7-3; Q9Y6N7-2 0 3 0.1951 0 1 1 486.93004 1458.77557 1458.7758 -0.16 -8e-05 1149599.0 CID (Collision Induced Dissociation) MS2 4.899548 40 150.5 60.0 76.227 23774 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 145.7 151.1 112.8 161.2 143.4 161.1 171.4 162.8 142.4 152.8 Excluded by Method 3.69 1 0.0009597 0.004335 False High Sequest HT (A2) Unambiguous SANEFSTTPEDK [K].sANEFSTtPEDk.[H] N-Term(TMT6plex); T8(Phospho); K12(TMT6plex) 1 Q06315 Q06315 0 3 0.1718 0 1 1 621.96498 1863.88039 1863.87776 1.41 0.00088 532370.6875 CID (Collision Induced Dissociation) MS2 32.19259 30 57.8 60.0 65.2631 19698 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 38.3 53.2 69.8 42.3 49.5 82.0 41.7 59.0 82.7 59.2 Excluded by Method 2.62 1 0.0009597 0.004565 False High Sequest HT (A2) Unambiguous LRTNLQPLESTQSQDF [K].lRTNLQPLESTQsQDF.[-] N-Term(TMT6plex); S13(Phospho) 1 Q9Y248 Q9Y248 1 2 0.2289 0 1 1 1093.53791 2186.06854 2186.06919 -0.3 -0.00033 889423.0 CID (Collision Induced Dissociation) MS2 0.0 50 61.7 60.0 103.7054 34153 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 60.3 62.0 52.2 60.4 62.4 62.7 65.4 69.5 57.7 64.1 Excluded by Method 2.84 1 0.0009597 0.004617 False High Sequest HT (A2) Unambiguous SPSLSPSPPSPLEK [K].sPSLSPSPPsPLEk.[T] N-Term(TMT6plex); S10(Phospho); K14(TMT6plex) 1 P46821 P46821 0 3 0.5121 0 1 1 654.35185 1961.04098 1961.03968 0.66 0.00043 3674690.0 CID (Collision Induced Dissociation) MS2 1.152511 40 176.1 21.665 88.6213 28423 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 179.7 175.4 146.7 186.2 177.4 172.1 194.2 193.8 171.5 164.1 Excluded by Method 4.12 1 0.0009597 0.004439 False High Sequest HT (A2) Unambiguous QEAGDSPPPAPGTPK [K].qEAGDsPPPAPGTPk.[A] N-Term(TMT6plex); S6(Phospho); K15(TMT6plex) 4 P49848 P49848-3; P49848; P49848-4; P49848-2 0 3 0.2228 0 1 1 663.00356 1986.99612 1986.99379 1.17 0.00078 407169.5 CID (Collision Induced Dissociation) MS2 30.9406 50 38.4 60.0 55.7583 16097 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 36.7 33.0 33.5 37.3 36.2 47.1 42.5 40.1 39.0 38.7 Excluded by Method 3.95 1 0.0009597 0.004367 False High Sequest HT (A2) Unambiguous TMFAQVESDDEEAK [K].tmFAQVEsDDEEAk.[N] N-Term(TMT6plex); M2(Oxidation); S8(Phospho); K14(TMT6plex) 1 Q9NW82 Q9NW82 0 2 0.2375 0 1 1 1077.48946 2153.97165 2153.9714 0.12 0.00012 402751.625 CID (Collision Induced Dissociation) MS2 0.0 30 36.0 60.0 74.4657 23169 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 40.3 30.9 32.3 43.4 30.7 37.6 45.2 34.2 39.1 26.0 Excluded by Method 2.61 1 0.0009597 0.004353 False High Sequest HT (A2) Unambiguous LAALESPPPVLK [K].lAALEsPPPVLk.[A] N-Term(TMT6plex); S6(Phospho); K12(TMT6plex) 1 P21576 P21576 0 3 0.2857 0 1 1 591.68224 1773.03216 1773.03275 -0.33 -0.0002 542590.9375 CID (Collision Induced Dissociation) MS2 21.17004 70 93.3 60.0 119.9635 40190 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 40.7 78.1 132.8 40.4 75.5 166.3 47.4 87.5 165.5 98.9 3.36 1 0.0009597 0.004575 False High Sequest HT (A2) Unambiguous LGDVSPTQIDVSQFGSFK [K].lGDVSPtQIDVSQFGSFk.[E] N-Term(TMT6plex); T7(Phospho); K18(TMT6plex) 1 P46821 P46821 0 3 0.1437 0 1 1 821.75708 2463.25668 2463.25728 -0.24 -0.0002 196459.609375 CID (Collision Induced Dissociation) MS2 19.59621 60 10.3 60.0 140.3242 47871 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 12.4 8.7 10.6 6.9 8.1 13.0 11.9 11.7 9.2 10.7 Excluded by Method 1.74 1 0.0009597 0.004451 False High Sequest HT (A2) Unambiguous ISLSPSTESLADSK [K].iSLSPStESLADSk.[S] N-Term(TMT6plex); T7(Phospho); K14(TMT6plex) 1 P31374 P31374 0 3 0.0208 0 1 1 658.34629 1973.0243 1973.02442 -0.06 -4e-05 966281.5625 CID (Collision Induced Dissociation) MS2 0.0 20 142.4 58.722 101.2967 33192 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 61.2 119.0 177.2 74.2 118.0 237.4 79.7 140.7 259.0 157.9 Excluded by Method 3.37 1 0.0009597 0.004428 False High Sequest HT (A2) Unambiguous AESDAVAEANDIDD [K].aESDAVAEANDIDD.[-] N-Term(TMT6plex) 1 P35180 P35180 0 2 0.3873 0 1 1 832.37876 1663.75025 1663.74962 0.38 0.00031 1501588.625 CID (Collision Induced Dissociation) MS2 0.0 40 32.8 60.0 68.5638 20941 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 11.7 30.2 43.3 15.0 26.0 57.9 18.2 29.6 60.7 35.1 Excluded by Method 2.04 1 0.0009597 0.004416 False High Sequest HT (A2) Unambiguous GYPVATPQK [K].gYPVAtPQk.[S] N-Term(TMT6plex); T6(Phospho); K9(TMT6plex) 1 P36421 P36421 0 3 0.4971 0 1 1 500.27424 1498.80817 1498.8071 0.71 0.00035 400572.6875 CID (Collision Induced Dissociation) MS2 10.45386 50 47.3 60.0 66.9189 20255 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 17.3 39.8 66.6 15.1 38.4 90.0 19.0 46.9 88.8 50.8 Excluded by Method 3.42 1 0.0009597 0.00452 False High Sequest HT (A2) Unambiguous VQGEAVSNIQENTQTPTVQEESEEEEVDETGVEVK [K].vQGEAVSNIQENTQTPTVQEESEEEEVDEtGVEVk.[D] N-Term(TMT6plex); T30(Phospho); K35(TMT6plex) 3 Q13765 Q13765; E9PAV3; E9PAV3-2 0 4 0.0026 0 1 1 1100.52111 4399.0626 4399.06016 0.56 0.00061 437752.46875 CID (Collision Induced Dissociation) MS2 0.0 40 19.5 60.0 134.8209 45965 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 21.1 20.0 20.5 17.4 16.8 19.0 20.1 18.9 21.3 19.7 Excluded by Method 3.8 1 0.0009597 0.004624 False High Sequest HT (A2) Unambiguous EEPPPARSGSGGGSAK [K].eEPPPARSGsGGGSAk.[E] N-Term(TMT6plex); S10(Phospho); K16(TMT6plex) 5 Q9H4A3 Q9H4A3-5; Q9H4A3-2; Q9H4A3-7; Q9H4A3; Q9H4A3-6 1 3 0.0605 0 1 1 674.67367 2022.00645 2022.00575 0.34 0.00023 1541656.125 CID (Collision Induced Dissociation) MS2 7.385297 30 82.6 60.0 36.9089 9251 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 79.9 81.0 70.2 80.0 77.3 88.5 92.1 82.7 83.0 91.4 Excluded by Method 2.81 1 0.0009597 0.00452 False High Sequest HT (A2) Unambiguous ENGSGDDLFAGLKK [K].eNGsGDDLFAGLkk.[K] N-Term(TMT6plex); S4(Phospho); K13(TMT6plex); K14(TMT6plex) 1 P09064 P09064 1 3 0.4302 0 1 1 740.06207 2218.17167 2218.17238 -0.32 -0.00024 721364.1875 CID (Collision Induced Dissociation) MS2 2.861715 10 49.3 60.0 105.6066 34774 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 11.6 41.4 68.5 16.7 42.0 102.1 15.2 47.8 90.5 56.7 Excluded by Method 3.44 1 0.0009597 0.004392 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVSsDEEF.[-] N-Term(TMT6plex); S9(Phospho) 1 P16521 P16521 0 2 0.0342 0 1 1 885.37192 1769.73657 1769.73562 0.53 0.00047 857363.75 CID (Collision Induced Dissociation) MS2 30.04099 60 61.8 60.0 123.5061 41431 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 25.3 49.7 101.3 20.2 50.9 108.3 24.4 56.9 119.5 61.2 Excluded by Method 3.22 1 0.0009597 0.004602 False High Sequest HT (A2) Unambiguous VVDYSQFQESDDADEDYGRDSGPPTK [K].vVDYSQFQEsDDADEDYGRDSGPPTk.[K] N-Term(TMT6plex); S10(Phospho); K26(TMT6plex) 1 Q9H1E3 Q9H1E3 1 4 0.0332 0 1 1 865.38709 3458.52652 3458.53134 -1.39 -0.0012 629860.25 CID (Collision Induced Dissociation) MS2 33.03444 50 12.0 60.0 132.638 45198 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 8.9 12.7 11.4 10.7 9.0 16.8 7.5 14.9 16.5 11.3 Excluded by Method 3.31 1 0.0009597 0.004553 False High Sequest HT (A2) Unambiguous EEEEAKEESDDDMGFGLFD [K].eEEEAkEEsDDDmGFGLFD.[-] N-Term(TMT6plex); K6(TMT6plex); S9(Phospho); M13(Oxidation) 1 P05318 P05318 1 3 0.6542 0 1 1 916.05036 2746.13654 2746.13669 -0.05 -5e-05 1568651.625 CID (Collision Induced Dissociation) MS2 5.569392 40 117.5 60.0 117.6718 39335 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 27.5 95.6 182.9 37.2 94.1 217.6 40.8 102.0 259.7 117.1 Excluded by Method 3.47 1 0.0009597 0.004504 False High Sequest HT (A2) Unambiguous EQLISTPIK [K].eQLIStPIk.[D] N-Term(TMT6plex); T6(Phospho); K9(TMT6plex) 1 P38692 P38692 0 3 0.1676 0 1 1 522.96835 1566.8905 1566.89083 -0.21 -0.00011 528603.1875 CID (Collision Induced Dissociation) MS2 56.01646 70 129.2 60.0 88.4923 28357 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 40.4 108.0 186.2 32.8 102.8 239.6 54.2 123.2 267.0 137.7 3.4 1 0.001134 0.004988 False High Sequest HT (A2) Unambiguous DDGSDRDSDREQDEK [K].dDGsDRDsDREQDEk.[Q] N-Term(TMT6plex); S4(Phospho); S8(Phospho); K15(TMT6plex) 1 Q9UGP8 Q9UGP8 2 3 0.5093 0 1 1 795.65938 2384.96358 2384.96449 -0.38 -0.0003 115874.4765625 CID (Collision Induced Dissociation) MS2 0.0 40 34.4 60.0 29.5868 6662 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 37.1 35.2 31.7 34.3 35.8 33.0 36.0 37.8 33.2 30.3 Excluded by Method 3.24 1 0.001134 0.004642 False High Sequest HT (A2) Unambiguous SSAESSNATNNNTLGTESK [K].sSAESsNATNNNTLGTESk.[L] N-Term(TMT6plex); S6(Phospho); K19(TMT6plex) 1 P38250 P38250 0 3 0.0517 0 1 1 817.38678 2450.1458 2450.14483 0.4 0.00032 1413386.25 CID (Collision Induced Dissociation) MS2 0.0 40 46.4 60.0 53.8674 15419 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 48.7 48.2 49.8 27.9 41.3 62.7 35.4 47.2 50.5 52.6 Excluded by Method 2.32 1 0.001134 0.004959 False High Sequest HT (A2) Unambiguous STPSDQYEEIAIIPTEPLHSDK [K].sTPSDQyEEIAIIPTEPLHSDk.[E] N-Term(TMT6plex); Y7(Phospho); K22(TMT6plex) 1 Q12344 Q12344 0 3 0.0064 0 1 1 1003.5041 3008.49775 3008.49058 2.38 0.00239 540229.0625 CID (Collision Induced Dissociation) MS2 0.0 60 15.5 60.0 115.3233 38389 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 8.0 14.3 20.5 3.9 11.5 31.7 4.0 17.3 28.1 16.1 Excluded by Method 3.14 1 0.001134 0.004738 False High Sequest HT (A2) Unambiguous EINFDDDF [K].eINFDDDF.[-] N-Term(TMT6plex) 1 P49723 P49723 0 2 0.6825 0 1 1 622.28798 1243.56868 1243.56801 0.54 0.00033 1134173.25 CID (Collision Induced Dissociation) MS2 32.27856 90 145.9 60.0 113.0851 37637 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 41.1 126.9 232.0 42.7 125.1 286.8 53.0 127.9 277.4 146.0 2.52 1 0.001134 0.004946 False High Sequest HT (A2) Unambiguous NSEDDELAK [K].nsEDDELAk.[E] N-Term(TMT6plex); S2(Phospho); K9(TMT6plex) 3 Q96T23 Q96T23; Q96T23-3; Q96T23-2 0 2 0.5 0 1 1 779.87395 1558.74063 1558.7402 0.27 0.00021 1601328.875 CID (Collision Induced Dissociation) MS2 4.679832 40 41.4 60.0 59.8975 17630 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 32.5 39.9 46.1 37.9 41.4 41.5 35.3 45.5 52.4 41.8 Excluded by Method 2.84 1 0.001134 0.004633 False High Sequest HT (A2) Unambiguous DVTPPPETEVVLIK [K].dVtPPPETEVVLIk.[N] N-Term(TMT6plex); T3(Phospho); K14(TMT6plex) 3 P27816 P27816; P27816-6; P27816-2 0 3 0.2116 0 1 1 692.38648 2075.14488 2075.14415 0.35 0.00024 15720270.0 CID (Collision Induced Dissociation) MS2 0.2780943 20 109.2 8.377 117.2724 39183 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 116.1 108.2 86.9 113.4 97.0 109.8 127.2 115.5 112.4 105.9 Excluded by Method 3.78 1 0.001134 0.004796 False High Sequest HT (A2) Unambiguous GTDTQTPAVLSPSK [K].gTDTQTPAVLSPsk.[T] N-Term(TMT6plex); S13(Phospho); K14(TMT6plex) 3 P46087 P46087-2; P46087; P46087-4 0 4 0.1098 0 1 1 485.75873 1940.0131 1940.01419 -0.56 -0.00027 1209493.25 CID (Collision Induced Dissociation) MS2 11.5435 30 104.4 60.0 73.5282 22721 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 95.7 100.1 83.9 97.2 104.5 104.6 125.0 112.5 103.5 117.3 Excluded by Method 1.73 1 0.001134 0.004654 False High Sequest HT (A2) Unambiguous EDSAPVAAGESESSL [K].eDsAPVAAGESESSL.[-] N-Term(TMT6plex); S3(Phospho) 1 P25567 P25567 0 2 0.5191 0 1 1 879.38757 1757.76785 1757.76799 -0.07 -7e-05 4541619.0 CID (Collision Induced Dissociation) MS2 4.790456 70 47.8 60.0 76.979 24029 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 11.0 35.1 74.1 20.8 38.5 84.6 24.4 49.9 97.4 42.1 2.62 1 0.001134 0.004768 False High Sequest HT (A2) Unambiguous AEDGATPSPSNETPK [K].aEDGAtPSPSNEtPk.[K] N-Term(TMT6plex); T6(Phospho); T13(Phospho); K15(TMT6plex) 1 P29966 P29966 0 3 0.1834 0 1 1 706.98483 2118.93994 2118.93978 0.07 5e-05 1183334.0 CID (Collision Induced Dissociation) MS2 0.0 70 31.0 60.0 51.3771 14500 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 24.2 29.7 24.6 34.0 31.3 30.6 37.9 34.2 34.9 28.5 2.29 1 0.001134 0.004632 False High Sequest HT (A2) Unambiguous RISICSSDK [K].rIsIcSSDk.[R] N-Term(TMT6plex); S3(Phospho); C5(Carbamidomethyl); K9(TMT6plex) 1 Q13547 Q13547 1 3 0.4724 0 1 1 535.28093 1603.82824 1603.82791 0.2 0.00011 1408562.375 CID (Collision Induced Dissociation) MS2 26.23471 30 128.4 60.0 61.4992 18290 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 126.3 143.4 101.6 134.4 116.6 119.8 150.6 138.8 129.0 123.4 Excluded by Method 2.54 1 0.001134 0.004679 False High Sequest HT (A2) Unambiguous NLLSSSDSRRNSQDEDSLPNNTNLIK [K].nLLSSSDsRRNsQDEDSLPNNTNLIk.[E] N-Term(TMT6plex); S8(Phospho); S12(Phospho); K26(TMT6plex) 1 Q08280 Q08280 2 4 0.0599 0 1 1 884.67558 3535.68049 3535.68312 -0.74 -0.00066 857656.125 CID (Collision Induced Dissociation) MS2 0.0 60 13.2 60.0 98.4394 32186 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 4.0 7.8 17.1 8.0 11.0 23.2 4.2 11.9 26.3 18.5 Excluded by Method 2.84 1 0.001134 0.00464 False High Sequest HT (A2) Unambiguous SLHPWYGITPTSSPK [K].sLHPWYGItPTSsPk.[T] N-Term(TMT6plex); T9(Phospho); S13(Phospho); K15(TMT6plex) 1 Q8N3F8 Q8N3F8 0 3 0.0113 0 1 1 763.70851 2289.11098 2289.11221 -0.54 -0.00041 1032587.375 CID (Collision Induced Dissociation) MS2 6.479334 40 117.4 60.0 106.0533 34979 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 109.2 129.0 98.3 120.5 118.5 117.8 134.2 121.4 110.5 114.7 Excluded by Method 4.41 1 0.001134 0.004927 False High Sequest HT (A2) Unambiguous DTCIQSPSK [K].dTcIQsPSk.[E] N-Term(TMT6plex); C3(Carbamidomethyl); S6(Phospho); K9(TMT6plex) 2 Q03188 Q03188; Q03188-2 0 3 0.2271 0 1 1 525.26165 1573.77041 1573.76973 0.43 0.00023 941592.625 CID (Collision Induced Dissociation) MS2 12.9926 60 41.4 60.0 53.4626 15297 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 42.8 37.1 34.5 40.8 41.7 45.9 42.1 44.4 41.6 43.4 Excluded by Method 2.73 1 0.001134 0.004836 False High Sequest HT (A2) Unambiguous YDSPVSSPITSASELGSIAK [K].yDsPVSSPITSASELGSIAk.[L] N-Term(TMT6plex); S3(Phospho); K20(TMT6plex) 1 P53890 P53890 0 3 0.0128 0 1 1 849.77175 2547.30069 2547.29954 0.45 0.00038 445102.84375 CID (Collision Induced Dissociation) MS2 0.0 60 33.8 60.0 120.3588 40336 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 13.9 24.6 49.9 10.9 28.3 60.6 14.7 38.9 66.0 30.5 Excluded by Method 2.34 1 0.001134 0.0047 False High Sequest HT (A2) Unambiguous DLFDYSPPLHK [K].dLFDYsPPLHk.[N] N-Term(TMT6plex); S6(Phospho); K11(TMT6plex) 3 Q9NYF8 Q9NYF8-3; Q9NYF8; Q9NYF8-2 0 3 0.0556 0 1 1 623.99037 1869.95656 1869.95522 0.71 0.00044 891792.875 CID (Collision Induced Dissociation) MS2 43.89 10 136.5 60.0 109.553 36317 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 128.8 134.2 131.5 137.5 132.0 147.8 135.3 143.5 150.9 123.9 Excluded by Method 2.88 1 0.001134 0.004861 False High Sequest HT (A2) Unambiguous DSLSRYDSDGDK [K].dSLSRYDsDGDk.[S] N-Term(TMT6plex); S8(Phospho); K12(TMT6plex) 7 Q04726 Q04726-5; Q04726-3; Q04726-2; Q04726-4; Q04726-7; Q04726-6; Q04726 1 3 0.3068 0 1 1 632.63099 1895.8784 1895.87882 -0.22 -0.00014 2539016.5 CID (Collision Induced Dissociation) MS2 32.47909 30 113.1 60.0 55.9164 16207 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 112.4 110.3 85.8 124.2 110.4 110.0 131.1 130.9 105.6 110.1 Excluded by Method 3.52 1 0.001134 0.004892 False High Sequest HT (A2) Unambiguous QVNEDADSDLL [K].qVNEDADsDLL.[-] N-Term(TMT6plex); S8(Phospho) 1 P38853 P38853 0 2 0.5089 0 1 1 764.34236 1527.67744 1527.67771 -0.18 -0.00014 1583577.875 CID (Collision Induced Dissociation) MS2 34.13828 40 79.9 60.0 91.6775 29582 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 25.5 62.6 112.5 26.4 73.9 150.2 29.8 69.4 158.0 91.2 Excluded by Method 2.24 1 0.001134 0.004943 False High Sequest HT (A2) Unambiguous IELSDDSDVEVHPNVDKK [K].iELSDDsDVEVHPNVDkk.[S] N-Term(TMT6plex); S7(Phospho); K17(TMT6plex); K18(TMT6plex) 1 P06101 P06101 1 4 0.0901 0 1 1 702.36741 2806.44779 2806.44788 -0.03 -2e-05 3844382.75 CID (Collision Induced Dissociation) MS2 7.730742 10 36.7 27.882 85.381 27210 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 15.5 34.2 48.2 18.7 40.3 53.7 22.2 30.8 64.5 39.0 Excluded by Method 3.33 1 0.001134 0.004709 False High Sequest HT (A2) Unambiguous SSLESPTMLK [K].sSLEsPTmLk.[L] N-Term(TMT6plex); S5(Phospho); M8(Oxidation); K10(TMT6plex) 1 Q12044 Q12044 0 3 0.0904 0 1 1 549.62085 1646.84798 1646.84765 0.2 0.00011 2898802.0 CID (Collision Induced Dissociation) MS2 5.979723 60 127.8 43.318 76.5399 23894 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 29.3 106.5 198.1 43.4 108.8 241.4 50.1 116.2 244.8 139.1 Excluded by Method 3.54 1 0.001134 0.004918 False High Sequest HT (A2) Unambiguous ATISDEEIERQLK [K].aTIsDEEIERQLk.[A] N-Term(TMT6plex); S4(Phospho); K13(TMT6plex) 2 Q9UQN3 Q9UQN3; Q9UQN3-2 1 3 0.1024 0 1 1 690.70132 2070.08942 2070.08842 0.48 0.00033 14601270.0 CID (Collision Induced Dissociation) MS2 0.0 20 90.6 12.096 90.7172 29189 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 87.6 99.7 67.0 85.2 85.5 82.3 102.6 99.6 93.3 102.8 Excluded by Method 4.2 1 0.001318 0.005087 False High Sequest HT (A2) Unambiguous SAEGNNIDQSLILK [K].sAEGNNIDQSLILk.[E] N-Term(TMT6plex); S1(Phospho); K14(TMT6plex) 1 P39705 P39705 0 3 0.204 0 1 1 680.69709 2040.0767 2040.07786 -0.56 -0.00038 1094234.125 CID (Collision Induced Dissociation) MS2 11.00538 40 97.4 60.0 104.0842 34245 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 26.6 84.6 146.0 31.3 83.6 172.7 35.6 90.4 193.6 109.7 Excluded by Method 2.5 1 0.001318 0.005063 False High Sequest HT (A2) Unambiguous WYPSEDVAALK [K].wyPSEDVAALk.[K] N-Term(TMT6plex); Y2(Phospho); K11(TMT6plex) 1 Q02326 Q02326 0 3 0.0306 0 1 1 606.31418 1816.92799 1816.92867 -0.38 -0.00023 873773.3125 CID (Collision Induced Dissociation) MS2 0.0 10 95.3 60.0 116.8612 39031 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 23.2 75.3 144.1 30.5 71.5 190.4 36.5 90.3 188.6 102.8 Excluded by Method 3.59 1 0.001318 0.005069 False High Sequest HT (A2) Unambiguous AYVRLTADYDALDIANRIGYI [K].aYVRLTADYDALDIANRIGYI.[-] N-Term(TMT6plex) 1 P04456 P04456 2 3 0.0857 0 1 1 872.47236 2615.40252 2615.40307 -0.21 -0.00018 12210334.0 CID (Collision Induced Dissociation) MS2 1.499988 40 7.1 10.595 128.2514 43252 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 1.4 2.6 13.1 1.7 8.9 15.4 8.6 13.3 6.4 Excluded by Method 2.1 1 0.001318 0.005003 False High Sequest HT (A2) Unambiguous DLPSLGSNAAFANVK [K].dLPsLGSNAAFANVk.[V] N-Term(TMT6plex); S4(Phospho); K15(TMT6plex) 1 P06105 P06105 0 3 0.0203 0 1 1 681.36305 2042.0746 2042.07238 1.09 0.00074 565376.8125 CID (Collision Induced Dissociation) MS2 5.484979 50 89.3 60.0 116.1304 38759 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 62.7 79.3 93.7 76.1 83.7 106.2 82.3 92.6 121.2 95.4 Excluded by Method 2.95 1 0.001505 0.0051 False High Sequest HT (A2) Unambiguous RLSLGAQK [K].rLsLGAQk.[G] N-Term(TMT6plex); S3(Phospho); K8(TMT6plex) 2 Q9H788 Q9H788; Q9H788-2 1 3 0.4116 0 1 1 470.94585 1410.82301 1410.82342 -0.29 -0.00014 5406465.5 CID (Collision Induced Dissociation) MS2 8.332339 60 75.7 38.991 64.9659 19590 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 73.5 64.3 59.1 83.5 72.4 71.7 77.2 84.7 86.3 84.4 Excluded by Method 2.77 1 0.001684 0.005103 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVssDEEF.[-] N-Term(TMT6plex); S8(Phospho); S9(Phospho) 1 P16521 P16521 0 2 0.1613 0 1 1 925.35532 1849.70336 1849.70195 0.76 0.0007 837326.25 CID (Collision Induced Dissociation) MS2 26.09548 40 86.4 53.305 132.6234 45166 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 23.4 75.1 136.5 24.3 73.8 164.9 33.3 75.0 164.5 93.4 Excluded by Method 3.1 1 0.001684 0.005152 False High Sequest HT (A2) Unambiguous QSLGELIGTLNAAK [K].qsLGELIGTLNAAk.[V] N-Term(TMT6plex); S2(Phospho); K14(TMT6plex) 2 P60174 P60174; P60174-3 0 3 0.2827 0 1 1 651.69914 1953.08287 1953.08221 0.34 0.00022 26353986.0 CID (Collision Induced Dissociation) MS2 4.871001 50 45.6 4.929 128.954 43653 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 32.9 43.6 58.8 23.7 38.2 71.3 33.4 45.1 67.8 41.5 Excluded by Method 1.91 1 0.001684 0.005176 False High Sequest HT (A2) Unambiguous VGILPSSSTDDVTSK [K].vGILPSSStDDVTSk.[E] N-Term(TMT6plex); T9(Phospho); K15(TMT6plex) 1 P22215 P22215 0 3 0.0114 0 1 1 682.02553 2044.06203 2044.06154 0.24 0.00016 960411.9375 CID (Collision Induced Dissociation) MS2 0.0 60 170.6 60.0 98.4582 32194 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 56.8 136.7 260.6 62.0 150.7 319.6 59.0 154.2 319.5 186.5 Excluded by Method 3.51 1 0.001684 0.005189 False High Sequest HT (A2) Unambiguous SIPEEQREQSPNK [K].sIPEEQREQsPNk.[M] N-Term(TMT6plex); S10(Phospho); K13(TMT6plex) 1 P40563 P40563 1 3 0.4661 0 1 1 694.02031 2080.04638 2080.04762 -0.6 -0.00041 275504.65625 CID (Collision Induced Dissociation) MS2 8.523803 40 90.0 60.0 48.6876 13514 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 20.7 72.1 126.5 30.3 84.3 173.4 33.2 87.8 175.1 96.4 Excluded by Method 3.69 1 0.001684 0.005103 False High Sequest HT (A2) Unambiguous NAEEESESEAEEGD [K].nAEEEsEsEAEEGD.[-] N-Term(TMT6plex); S6(Phospho); S8(Phospho) 4 Q7L1Q6 Q7L1Q6-2; Q7L1Q6-4; Q7L1Q6-3; Q7L1Q6 0 2 0.9154 0 1 1 957.32438 1913.64147 1913.6412 0.14 0.00013 104845.078125 CID (Collision Induced Dissociation) MS2 0.0 60 8.3 60.0 42.9524 11384 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 7.3 7.9 3.9 7.8 11.8 6.8 10.9 7.8 11.6 6.9 Excluded by Method 2.72 1 0.001684 0.005139 False High Sequest HT (A2) Unambiguous EINFDDDF [K].eINFDDDF.[-] N-Term(TMT6plex) 1 P49723 P49723 0 2 0.7016 0 1 1 622.28806 1243.56884 1243.56801 0.66 0.00041 1035420.8125 CID (Collision Induced Dissociation) MS2 37.17407 90 172.1 60.0 113.504 37723 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 44.5 134.9 290.9 49.9 135.7 344.0 59.4 151.1 333.2 176.9 2.48 1 0.001684 0.00518 False High Sequest HT (A2) Unambiguous ISPDLNIK [K].isPDLNIk.[C] N-Term(TMT6plex); S2(Phospho); K8(TMT6plex) 1 Q69YH5 Q69YH5 0 2 0.2423 0 1 1 719.40928 1437.81128 1437.81185 -0.4 -0.00029 4637715.5 CID (Collision Induced Dissociation) MS2 11.27453 30 94.2 60.0 100.4611 32854 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 92.2 99.7 80.5 101.2 89.3 89.5 95.9 96.8 94.5 102.3 Excluded by Method 2.93 1 0.001684 0.005115 False High Sequest HT (A2) Unambiguous NYGSPLISGSTPK [K].nYGSPLISGStPk.[H] N-Term(TMT6plex); T11(Phospho); K13(TMT6plex) 1 Q15648 Q15648 0 3 0.0377 0 1 1 620.32864 1858.97136 1858.9716 -0.13 -8e-05 1546921.5 CID (Collision Induced Dissociation) MS2 11.30884 30 69.9 60.0 85.2753 27172 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 69.1 64.1 52.8 71.5 66.2 75.9 77.9 70.8 90.2 60.4 Excluded by Method 3.18 1 0.001684 0.005146 False High Sequest HT (A2) Unambiguous EEEAAEESDDDMGFGLFD [K].eEEAAEEsDDDmGFGLFD.[-] N-Term(TMT6plex); S8(Phospho); M12(Oxidation) 2 P05319 P10622; P05319 0 2 0.7713 0 1 1 1165.94216 2330.87705 2330.87332 1.6 0.00187 845854.1875 CID (Collision Induced Dissociation) MS2 53.88636 50 47.2 60.0 147.2298 50257 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 13.2 37.2 78.7 13.7 42.6 85.7 17.8 45.8 90.8 46.3 Excluded by Method 3.28 1 0.001684 0.005113 False High Sequest HT (A2) Unambiguous TPLGERSVNFSLTPNEIK [K].tPLGERSVNFSLtPNEIk.[V] N-Term(TMT6plex); T13(Phospho); K18(TMT6plex) 1 P46821 P46821 1 3 0.071 0 1 1 847.45595 2540.35331 2540.35258 0.29 0.00025 1195477.25 CID (Collision Induced Dissociation) MS2 50.25045 30 20.3 60.0 108.3433 35861 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 13.7 17.6 22.7 19.4 19.2 21.8 14.9 24.4 29.9 19.7 Excluded by Method 3.1 1 0.001796 0.005676 False High Sequest HT (A2) Unambiguous SSLESPTMLK [K].sSLEsPTmLk.[L] N-Term(TMT6plex); S5(Phospho); M8(Oxidation); K10(TMT6plex) 1 Q12044 Q12044 0 2 0.0582 0 1 1 823.92741 1646.84755 1646.84765 -0.06 -5e-05 1015957.25 CID (Collision Induced Dissociation) MS2 10.85852 20 159.9 60.0 77.2699 24222 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 47.6 136.1 238.3 55.6 131.8 302.6 78.1 138.5 311.7 158.3 Excluded by Method 2.92 1 0.001796 0.005496 False High Sequest HT (A2) Unambiguous IFESILNSPSSK [K].iFESILNsPSSk.[S] N-Term(TMT6plex); S8(Phospho); K12(TMT6plex) 1 P06105 P06105 0 3 0.1322 0 1 1 620.66879 1859.9918 1859.992 -0.11 -7e-05 263994.53125 CID (Collision Induced Dissociation) MS2 11.19521 50 20.8 60.0 124.4435 41779 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 11.4 18.3 29.9 9.5 20.1 30.7 13.7 20.3 33.4 20.2 Excluded by Method 2.95 1 0.001796 0.00561 False High Sequest HT (A2) Unambiguous SFKLSGFSFK [K].sFkLsGFSFk.[K] N-Term(TMT6plex); K3(TMT6plex); S5(Phospho); K10(TMT6plex) 1 P29966 P29966 1 3 0.2143 0 1 1 639.02789 1915.06912 1915.06975 -0.33 -0.00021 5610298.5 CID (Collision Induced Dissociation) MS2 5.827425 10 181.3 24.925 119.3501 39914 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 195.9 179.8 140.9 199.3 184.6 178.7 212.1 195.3 151.5 175.0 Excluded by Method 3.5 1 0.001796 0.006267 False High Sequest HT (A2) Unambiguous ALSYASLDK [K].aLsYAsLDk.[E] N-Term(TMT6plex); S3(Phospho); S6(Phospho); K9(TMT6plex) 2 Q6R327 Q6R327; Q6R327-3 0 2 0.1908 0 1 1 793.38826 1585.76925 1585.76801 0.78 0.00062 1265722.875 CID (Collision Induced Dissociation) MS2 42.07945 10 132.7 60.0 96.5843 31479 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 133.2 125.1 108.6 149.8 124.4 132.6 156.2 121.8 137.0 138.7 Excluded by Method 3.25 1 0.001796 0.005544 False High Sequest HT (A2) Unambiguous ESQIDVLQQDELDSYPDSVK [K].eSQIDVLQQDELDsYPDSVk.[K] N-Term(TMT6plex); S14(Phospho); K20(TMT6plex) 1 Q9NQS3 Q9NQS3 0 3 0.0413 0 1 1 949.463 2846.37445 2846.37489 -0.15 -0.00015 440118.71875 CID (Collision Induced Dissociation) MS2 60.49798 60 19.2 60.0 111.9245 37135 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 16.8 19.4 16.1 15.6 18.2 19.7 19.1 24.2 21.1 21.5 Excluded by Method 3.63 1 0.001796 0.006117 False High Sequest HT (A2) Unambiguous LPANIVELDLHESPESVK [K].lPANIVELDLHESPEsVk.[I] N-Term(TMT6plex); S16(Phospho); K18(TMT6plex) 1 P23201 P23201 0 3 0.0044 0 1 1 843.45151 2528.33997 2528.34134 -0.54 -0.00046 985386.25 CID (Collision Induced Dissociation) MS2 13.78824 30 57.9 60.0 120.8468 40463 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 25.6 46.9 79.2 24.9 49.6 101.8 29.7 49.2 104.0 68.2 Excluded by Method 4.52 1 0.001796 0.005919 False High Sequest HT (A2) Unambiguous YDSPVSSPITSASELGSIAK [K].yDSPVSsPITSASELGSIAk.[L] N-Term(TMT6plex); S7(Phospho); K20(TMT6plex) 1 P53890 P53890 0 3 0.0289 0 1 1 849.77157 2547.30015 2547.29954 0.24 0.0002 458202.71875 CID (Collision Induced Dissociation) MS2 23.88527 40 32.4 60.0 121.0823 40548 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 11.8 24.0 46.1 16.0 26.1 56.6 16.9 34.4 59.1 33.0 Excluded by Method 1.73 1 0.001796 0.00567 False High Sequest HT (A2) Unambiguous VQGEAVSNIQENTQTPTVQEESEEEEVDETGVEVK [K].vQGEAVSNIQENTQTPtVQEESEEEEVDETGVEVk.[D] N-Term(TMT6plex); T17(Phospho); K35(TMT6plex) 3 Q13765 Q13765; E9PAV3; E9PAV3-2 0 4 0.0259 0 1 1 1100.5224 4399.06777 4399.06016 1.73 0.0019 469831.78125 CID (Collision Induced Dissociation) MS2 25.46086 50 8.4 60.0 140.9664 48095 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 7.0 7.4 7.6 9.0 3.8 11.1 11.2 8.3 12.1 6.4 Excluded by Method 3.47 1 0.001796 0.005662 False High Sequest HT (A2) Unambiguous DESDSENDIEIK [K].dESDsENDIEIk.[G] N-Term(TMT6plex); S5(Phospho); K12(TMT6plex) 1 P32499 P32499 0 3 0.0346 0 1 1 644.63616 1931.89393 1931.88872 2.7 0.00174 896282.125 CID (Collision Induced Dissociation) MS2 30.6433 50 52.0 60.0 73.9468 22975 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 31.7 45.8 65.9 36.0 43.4 77.2 31.2 56.0 77.8 55.2 Excluded by Method 3.47 1 0.001796 0.006069 False High Sequest HT (A2) Unambiguous AVEARSPGLQK [K].aVEARsPGLQk.[E] N-Term(TMT6plex); S6(Phospho); K11(TMT6plex) 4 Q3KQU3 Q3KQU3-3; Q3KQU3-2; Q3KQU3-4; Q3KQU3 1 3 0.2943 0 1 1 565.31843 1693.94075 1693.94024 0.3 0.00017 2489120.25 CID (Collision Induced Dissociation) MS2 2.502547 30 128.3 60.0 55.3611 16002 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 122.6 130.9 149.5 83.1 115.0 159.3 122.7 125.4 116.1 158.5 Excluded by Method 2.65 1 0.001796 0.00524 False High Sequest HT (A2) Unambiguous VVDYSQFQESDDADEDYGRDSGPPTK [K].vVDYSQFQESDDADEDyGRDSGPPTk.[K] N-Term(TMT6plex); Y17(Phospho); K26(TMT6plex) 1 Q9H1E3 Q9H1E3 1 5 0.0028 0 1 1 692.51256 3458.5337 3458.53134 0.68 0.00047 541094.4375 CID (Collision Induced Dissociation) MS2 4.426484 40 29.4 60.0 90.1708 29012 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 25.5 28.0 22.4 31.0 29.8 30.8 31.4 30.9 30.3 34.3 Excluded by Method 3.53 1 0.001796 0.005662 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVssDEEF.[-] N-Term(TMT6plex); S8(Phospho); S9(Phospho) 1 P16521 P16521 0 2 0.1422 0 1 1 925.35477 1849.70226 1849.70195 0.16 0.00015 3223097.25 CID (Collision Induced Dissociation) MS2 28.21522 60 44.7 19.222 129.0442 43625 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 21.1 41.6 52.0 18.8 41.0 81.6 25.0 45.1 79.2 41.2 Excluded by Method 2.18 1 0.001796 0.00559 False High Sequest HT (A2) Unambiguous MDTCSSNLNNSIYK [K].mDTcSSNLNNsIYk.[K] N-Term(TMT6plex); C4(Carbamidomethyl); S11(Phospho); K14(TMT6plex) 1 P25116 P25116 0 3 0.2065 0 1 1 729.00649 2185.00492 2185.00708 -0.99 -0.00072 1183251.875 CID (Collision Induced Dissociation) MS2 9.502645 30 38.2 60.0 81.4954 25723 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 39.1 30.2 35.3 34.9 35.7 40.7 43.2 42.8 39.1 40.9 Excluded by Method 3.1 1 0.001796 0.005917 False High Sequest HT (A2) Unambiguous ESEILERTPSPLK [K].eSEILERtPsPLk.[K] N-Term(TMT6plex); T8(Phospho); S10(Phospho); K13(TMT6plex) 1 P40157 P40157 1 3 0.2092 0 1 1 706.3624 2117.07263 2117.06967 1.4 0.00099 1031888.125 CID (Collision Induced Dissociation) MS2 15.01504 40 126.6 60.0 86.7606 27731 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 33.8 107.8 199.3 41.5 107.3 220.7 50.8 122.8 252.6 129.1 Excluded by Method 2.39 1 0.001796 0.005447 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVSsDEEF.[-] N-Term(TMT6plex); S9(Phospho) 1 P16521 P16521 0 2 0.007 0 1 1 885.37053 1769.73378 1769.73562 -1.04 -0.00092 646572.25 CID (Collision Induced Dissociation) MS2 15.03864 50 63.8 60.0 133.442 45492 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 20.0 58.6 92.7 24.8 50.1 121.1 26.6 60.8 122.0 61.6 Excluded by Method 2.87 1 0.001796 0.005572 False High Sequest HT (A2) Unambiguous LDESDENEEALAK [K].lDEsDENEEALAk.[A] N-Term(TMT6plex); S4(Phospho); K13(TMT6plex) 1 Q08492 Q08492 0 3 0.5306 0 1 1 667.65388 2000.94709 2000.94657 0.26 0.00017 11327410.0 CID (Collision Induced Dissociation) MS2 0.0 20 106.3 11.387 82.2401 26008 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 26.6 83.7 162.9 31.3 85.3 199.5 37.1 105.7 224.6 106.7 Excluded by Method 3.92 1 0.001796 0.005646 False High Sequest HT (A2) Unambiguous MNGRKQSLGELIGTLNAAK [K].mNGRkQsLGELIGTLNAAk.[V] N-Term(TMT6plex); K5(TMT6plex); S7(Phospho); K19(TMT6plex) 2 P60174 P60174; P60174-3 2 4 0.4107 0 1 1 692.89218 2768.54688 2768.5461 0.28 0.0002 335006.90625 CID (Collision Induced Dissociation) MS2 11.78134 30 48.4 60.0 114.4813 38083 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 54.7 49.5 36.4 46.3 43.6 54.4 54.4 45.7 52.9 46.2 Excluded by Method 3.19 1 0.001796 0.006201 False High Sequest HT (A2) Unambiguous DISGPSPSK [K].dISGPsPSk.[K] N-Term(TMT6plex); S6(Phospho); K9(TMT6plex) 2 Q96GX9 Q96GX9; Q96GX9-3 0 2 0.1596 0 1 1 713.37345 1425.73963 1425.73908 0.39 0.00027 15836510.0 CID (Collision Induced Dissociation) MS2 2.705933 40 225.3 16.07 58.2329 17059 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 217.4 208.8 185.9 230.5 217.0 222.2 259.5 252.8 212.2 246.7 Excluded by Method 3.07 1 0.001796 0.005351 False High Sequest HT (A2) Unambiguous VVRNNSITSATSK [K].vVRNNsITSATSk.[T] N-Term(TMT6plex); S6(Phospho); K13(TMT6plex) 1 Q05050 Q05050 1 3 0.3224 0 1 1 639.01898 1915.04239 1915.04141 0.51 0.00032 5384020.0 CID (Collision Induced Dissociation) MS2 4.318123 50 23.7 39.54 57.264 16707 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 10.0 21.9 28.8 11.1 18.0 42.2 10.9 22.9 46.5 24.2 Excluded by Method 2.45 1 0.001796 0.005876 False High Sequest HT (A2) Unambiguous NTSSPPISPNAAAIQEEDSSK [K].nTSsPPIsPNAAAIQEEDSSk.[K] N-Term(TMT6plex); S4(Phospho); S8(Phospho); K21(TMT6plex) 1 P40091 P40091 0 4 0.2071 0 1 1 691.07384 2761.27354 2761.27347 0.02 2e-05 920318.75 CID (Collision Induced Dissociation) MS2 17.29475 30 34.5 60.0 85.2381 27158 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 12.3 29.8 48.1 15.1 29.8 64.9 12.3 32.4 60.7 39.8 Excluded by Method 4.2 1 0.001796 0.005389 False High Sequest HT (A2) Unambiguous VRYPPSPAK [K].vRYPPsPAk.[M] N-Term(TMT6plex); S6(Phospho); K9(TMT6plex) 1 P30838 P30838 1 3 0.349 0 1 1 518.29307 1552.86465 1552.86529 -0.41 -0.00021 561369.6875 CID (Collision Induced Dissociation) MS2 0.0 50 69.5 60.0 57.5818 16796 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 71.7 63.5 45.7 72.0 76.9 67.4 88.1 79.3 62.3 68.1 Excluded by Method 2.55 1 0.001796 0.005994 False High Sequest HT (A2) Unambiguous FVDDSDTSYEEK [K].fVDDSDTsYEEk.[E] N-Term(TMT6plex); S8(Phospho); K12(TMT6plex) 1 Q08951 Q08951 0 2 0.0537 0 1 1 986.94603 1972.88477 1972.88291 0.95 0.00093 1072478.125 CID (Collision Induced Dissociation) MS2 0.0 30 71.0 60.0 82.304 26032 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 24.9 64.8 87.2 26.1 52.9 133.4 34.2 63.7 139.5 83.7 Excluded by Method 2.98 1 0.001796 0.005733 False High Sequest HT (A2) Unambiguous VANSPTMVNLLLGK [K].vANSPtmVNLLLGk.[G] N-Term(TMT6plex); T6(Phospho); M7(Oxidation); K14(TMT6plex) 1 Q6UB99 Q6UB99 0 3 0.0226 0 1 1 671.04065 2011.10741 2011.10632 0.54 0.00036 922369.375 CID (Collision Induced Dissociation) MS2 5.803096 20 50.9 60.0 117.7411 39277 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 51.8 50.6 50.1 50.4 48.2 51.6 54.5 56.4 52.8 42.9 Excluded by Method 2.21 1 0.001796 0.005961 False High Sequest HT (A2) Unambiguous EQLISTPIK [K].eQLIStPIk.[D] N-Term(TMT6plex); T6(Phospho); K9(TMT6plex) 1 P38692 P38692 0 3 0.2094 0 1 1 522.9685 1566.89096 1566.89083 0.08 4e-05 1112074.375 CID (Collision Induced Dissociation) MS2 20.45411 60 71.2 60.0 88.2916 28364 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 21.6 60.8 96.2 20.1 56.3 135.0 26.9 77.9 136.3 81.0 Excluded by Method 3.2 1 0.001796 0.005307 False High Sequest HT (A2) Unambiguous SVGFGLVYNSVAEAK [K].sVGFGLVYNSVAEAk.[K] N-Term(TMT6plex); K15(TMT6plex) 1 P0CX31 P0CX31 0 3 0.342 0 1 1 667.04812 1999.1298 1999.12645 1.68 0.00112 326632.59375 CID (Collision Induced Dissociation) MS2 52.25897 60 17.3 60.0 117.5232 39234 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 3.9 13.0 23.5 3.7 12.0 41.0 10.8 16.6 31.3 16.7 Excluded by Method 1.93 1 0.001796 0.006417 False High Sequest HT (A2) Unambiguous EEDEEPESPPEK [K].eEDEEPEsPPEk.[K] N-Term(TMT6plex); S8(Phospho); K12(TMT6plex) 2 Q9H1E3 Q9H1E3; Q9H1E3-2 0 4 0.159 0 1 1 488.97511 1952.87863 1952.87782 0.41 0.0002 2794609.75 CID (Collision Induced Dissociation) MS2 6.81873 60 202.0 60.0 54.8878 15802 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 198.5 187.7 156.1 224.5 200.0 208.4 218.6 209.7 212.0 204.9 Excluded by Method 3.71 1 0.001796 0.006376 False High Sequest HT (A2) Unambiguous KEPEESVEENTSK [K].kEPEESVEENTsk.[T] N-Term(TMT6plex); K1(TMT6plex); S12(Phospho); K13(TMT6plex) 1 Q01477 Q01477 1 3 0.0442 0 1 1 758.38936 2273.15354 2273.1517 0.81 0.00061 1431934.5 CID (Collision Induced Dissociation) MS2 0.0 50 75.1 60.0 54.8048 15771 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 26.1 60.1 110.7 35.6 69.6 115.9 32.0 70.6 140.7 90.2 Excluded by Method 3.62 1 0.001796 0.00643 False High Sequest HT (A2) Unambiguous TSPSPIRIEEEVK [K].tsPSPIRIEEEVk.[E] N-Term(TMT6plex); S2(Phospho); K13(TMT6plex) 3 Q8WYB5 Q8WYB5; Q8WYB5-3; Q8WYB5-2 1 3 0.4043 0 1 1 675.03413 2023.08784 2023.08769 0.07 5e-05 558674.875 CID (Collision Induced Dissociation) MS2 65.05486 30 52.4 60.0 88.8248 28502 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 47.5 54.3 44.1 45.2 45.6 63.0 53.9 55.0 62.5 52.8 Excluded by Method 1.88 1 0.001796 0.005967 False High Sequest HT (A2) Unambiguous ESEILERTPSPLK [K].eSEILERtPsPLk.[K] N-Term(TMT6plex); T8(Phospho); S10(Phospho); K13(TMT6plex) 1 P40157 P40157 1 3 0.1723 0 1 1 706.36168 2117.07049 2117.06967 0.39 0.00027 1254896.125 CID (Collision Induced Dissociation) MS2 10.11368 40 105.0 60.0 87.4694 28051 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 35.4 91.1 147.0 39.2 84.2 180.7 51.1 91.9 217.5 112.0 Excluded by Method 2.67 1 0.001796 0.005569 False High Sequest HT (A2) Unambiguous QEAGDSPPPAPGTPK [K].qEAGDsPPPAPGtPk.[A] N-Term(TMT6plex); S6(Phospho); T13(Phospho); K15(TMT6plex) 4 P49848 P49848-3; P49848; P49848-4; P49848-2 0 3 0.5214 0 1 1 689.65793 2066.95924 2066.96012 -0.43 -0.0003 668738.6875 CID (Collision Induced Dissociation) MS2 48.47031 60 27.8 60.0 55.7419 16091 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 31.3 27.3 26.3 31.1 22.7 30.4 28.7 25.9 27.3 27.0 Excluded by Method 3.97 1 0.001796 0.006142 False High Sequest HT (A2) Unambiguous TSTSPPPEK [K].tSTsPPPEk.[S] N-Term(TMT6plex); S4(Phospho); K9(TMT6plex) 2 Q9H1E3 Q9H1E3; Q9H1E3-2 0 3 0.0751 0 1 1 494.59314 1481.76486 1481.7653 -0.3 -0.00015 437354.5 CID (Collision Induced Dissociation) MS2 4.704991 40 316.0 60.0 44.4676 11977 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 333.3 315.4 265.3 322.2 286.1 316.6 364.1 322.6 320.5 313.9 Excluded by Method 2.13 1 0.001796 0.005538 False High Sequest HT (A2) Unambiguous EEEEAKEESDDDMGFGLFD [K].eEEEAkEEsDDDmGFGLFD.[-] N-Term(TMT6plex); K6(TMT6plex); S9(Phospho); M13(Oxidation) 1 P05318 P05318 1 3 0.7059 0 1 1 916.05084 2746.13795 2746.13669 0.46 0.00042 3005400.5 CID (Collision Induced Dissociation) MS2 0.0 50 129.8 54.855 115.3719 38444 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 29.0 112.9 178.1 41.2 103.1 224.5 51.7 128.1 290.6 139.0 Excluded by Method 3.74 1 0.001796 0.005555 False High Sequest HT (A2) Unambiguous HIKEEPLSEEEPCTSTAIASPEK [K].hIkEEPLSEEEPctSTAIAsPEk.[K] N-Term(TMT6plex); K3(TMT6plex); C13(Carbamidomethyl); T14(Phospho); S20(Phospho); K23(TMT6plex) 1 Q9Y2X3 Q9Y2X3 1 4 0.0038 0 1 1 858.16834 3429.65155 3429.6505 0.31 0.00026 943140.625 CID (Collision Induced Dissociation) MS2 3.466209 40 20.6 60.0 81.6422 25811 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 14.7 15.4 21.5 15.3 20.3 28.3 11.9 24.2 29.8 25.1 Excluded by Method 2.6 1 0.001796 0.006001 False High Sequest HT (A2) Unambiguous SPQPPVEEEDEHFDDTVVCLDTYNCDLHFK [K].sPQPPVEEEDEHFDDTVVcLDTYNcDLHFk.[I] N-Term(TMT6plex); S1(Phospho); C19(Carbamidomethyl); C25(Carbamidomethyl); K30(TMT6plex) 2 Q00839 Q00839-2; Q00839 0 4 0.5339 0 1 1 1044.21832 4173.85146 4173.84991 0.37 0.00039 856010.625 CID (Collision Induced Dissociation) MS2 11.26654 60 7.7 39.092 127.1825 42771 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 6.8 6.7 10.5 3.8 7.8 10.1 6.6 9.6 8.0 7.1 Excluded by Method 5.02 1 0.001796 0.005424 False High Sequest HT (A2) Unambiguous EPETRYSVLNNDDYFADVSPLRATSPSK [K].ePETRYSVLNNDDYFADVSPLRATsPSk.[S] N-Term(TMT6plex); S25(Phospho); K28(TMT6plex) 1 Q1ED39 Q1ED39 2 4 0.0218 0 1 1 928.21005 3709.81836 3709.81511 0.88 0.00081 3281138.5 CID (Collision Induced Dissociation) MS2 1.291222 30 43.1 39.329 111.5794 37005 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 44.9 44.9 30.9 39.4 46.6 45.4 44.2 46.2 40.7 47.6 Excluded by Method 3.67 1 0.001796 0.005623 False High Sequest HT (A2) Unambiguous STLESEKPGSPEAAETSPPSNIIDHCEK [K].sTLESEkPGsPEAAETsPPSNIIDHcEk.[L] N-Term(TMT6plex); K7(TMT6plex); S10(Phospho); S17(Phospho); C26(Carbamidomethyl); K28(TMT6plex) 3 Q96T23 Q96T23; Q96T23-3; Q96T23-2 0 4 0.1122 0 1 1 965.21025 3857.81919 3857.81606 0.81 0.00078 933051.5 CID (Collision Induced Dissociation) MS2 0.0 20 35.9 60.0 87.6855 28074 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 32.0 35.3 27.9 36.3 33.3 40.3 41.6 38.7 41.6 32.4 Excluded by Method 4.1 1 0.001796 0.006065 False High Sequest HT (A2) Unambiguous ALVLELDEMSNGSEIQDALEEISGQK [K].aLVLELDEmsNGSEIQDALEEISGQk.[T] N-Term(TMT6plex); M9(Oxidation); S10(Phospho); K26(TMT6plex) 2 P17695 P17695; P17695-2 0 3 0.0135 0 1 1 1124.89022 3372.65609 3372.65337 0.81 0.00091 232134.640625 CID (Collision Induced Dissociation) MS2 26.52388 60 15.1 60.0 139.4552 47571 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 6.9 13.2 23.0 3.6 15.0 24.2 7.6 14.2 27.6 16.1 Excluded by Method 2.23 1 0.001796 0.006248 False High Sequest HT (A2) Unambiguous YAAAAPAATSAASGDAAPAEEAAAEEEEESDDDMGFGLFD [K].yAAAAPAATSAASGDAAPAEEAAAEEEEEsDDDmGFGLFD.[-] N-Term(TMT6plex); S30(Phospho); M34(Oxidation) 1 P05317 P05317 0 4 0.3205 0 1 1 1062.19449 4245.75611 4245.73286 5.48 0.00581 281538.90625 CID (Collision Induced Dissociation) MS2 58.9765 50 21.0 60.0 143.1462 48886 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 3.8 23.3 37.2 6.4 14.7 36.9 6.5 16.4 39.2 25.1 Excluded by Method 3.37 1 0.001796 0.005866 False High Sequest HT (A2) Unambiguous VLPELQGK [K].vLPELQGk.[L] N-Term(TMT6plex); K8(TMT6plex) 3 P00359 P00360; P00359; P00358 0 2 0.4758 0 1 1 671.42914 1341.85101 1341.85061 0.3 0.0002 727020.0625 CID (Collision Induced Dissociation) MS2 42.86283 70 107.7 60.0 90.5365 29123 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 32.0 81.8 187.1 38.6 93.1 194.6 40.1 95.0 204.9 109.7 2.48 1 0.001796 0.006272 False High Sequest HT (A2) Unambiguous SPSLSPSPPSPLEK [K].sPSLSPsPPsPLEk.[T] N-Term(TMT6plex); S7(Phospho); S10(Phospho); K14(TMT6plex) 1 P46821 P46821 0 3 0.2969 0 1 1 681.00653 2041.00505 2041.00601 -0.47 -0.00032 793619.4375 CID (Collision Induced Dissociation) MS2 70.9723 30 108.1 56.283 92.8735 30031 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 109.4 94.2 89.9 110.9 93.3 119.0 128.6 116.2 104.8 114.7 Excluded by Method 3.2 1 0.001796 0.006313 False High Sequest HT (A2) Unambiguous DFELEETDEEK [K].dFELEEtDEEk.[A] N-Term(TMT6plex); T7(Phospho); K11(TMT6plex) 2 P15108 P02829; P15108 0 3 0.3413 0 1 1 641.29596 1921.87332 1921.87201 0.68 0.00044 338591.75 CID (Collision Induced Dissociation) MS2 23.00813 60 68.2 60.0 88.9388 28524 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 52.6 62.7 61.3 62.0 62.5 82.4 62.2 67.3 92.1 76.7 Excluded by Method 2.52 1 0.001796 0.006401 False High Sequest HT (A2) Unambiguous DSLQELSTEQK [K].dSLQELsTEQk.[C] N-Term(TMT6plex); S7(Phospho); K11(TMT6plex) 1 Q99567 Q99567 0 3 0.1012 0 1 1 605.97624 1815.91416 1815.91415 0.01 1e-05 895177.125 CID (Collision Induced Dissociation) MS2 7.35443 50 38.8 60.0 72.3947 22331 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 31.7 34.8 36.1 36.8 39.7 38.8 44.3 38.9 42.9 44.1 Excluded by Method 2.47 1 0.001796 0.005582 False High Sequest HT (A2) Unambiguous KTSTSPPPEK [K].kTSTsPPPEk.[S] N-Term(TMT6plex); K1(TMT6plex); S5(Phospho); K10(TMT6plex) 2 Q9H1E3 Q9H1E3; Q9H1E3-2 1 3 0.0773 0 1 1 613.6798 1839.02484 1839.02319 0.89 0.00055 1234029.375 CID (Collision Induced Dissociation) MS2 20.45778 30 289.5 60.0 46.3297 12615 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 337.3 293.6 237.2 314.2 276.2 263.2 345.4 296.4 246.9 284.9 Excluded by Method 2.07 1 0.001796 0.006222 False High Sequest HT (A2) Unambiguous VLEESSVK [K].vLEESsVk.[D] N-Term(TMT6plex); S6(Phospho); K8(TMT6plex) 1 P46949 P46949 0 3 0.1968 0 1 1 476.92995 1428.77529 1428.77513 0.11 5e-05 1198192.375 CID (Collision Induced Dissociation) MS2 3.642557 20 63.9 60.0 72.8328 22549 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 31.0 54.4 82.0 36.7 57.0 96.5 34.0 63.0 120.3 64.2 Excluded by Method 2.49 1 0.001796 0.005369 False High Sequest HT (A2) Unambiguous HTGPGILSMANAGPNTNGSQFFICTAK [K].hTGPGILSmANAGPNTNGSQFFIcTAk.[T] N-Term(TMT6plex); M9(Oxidation); C24(Carbamidomethyl); K27(TMT6plex) 2 P62937 P62937; P62937-2 0 4 0.1993 0 1 1 817.16797 3265.65006 3265.64982 0.07 6e-05 477026.78125 CID (Collision Induced Dissociation) MS2 56.94323 70 28.6 60.0 92.2191 29788 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 24.5 26.6 20.9 29.9 36.3 31.2 32.2 28.3 34.7 21.7 2.76 1 0.001796 0.006149 False High Sequest HT (A2) Unambiguous GPQPPTVSPIRSSRPPPAK [K].gPQPPTVsPIRSSRPPPAk.[R] N-Term(TMT6plex); S8(Phospho); K19(TMT6plex) 3 P46087 P46087-2; P46087; P46087-4 1 4 0.0682 0 1 1 627.60255 2507.38837 2507.38996 -0.64 -0.0004 587983.0 CID (Collision Induced Dissociation) MS2 50.23761 30 42.1 60.0 63.6215 19088 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 46.9 40.9 35.7 45.3 44.9 37.6 45.8 41.6 41.7 41.0 Excluded by Method 4.25 1 0.001796 0.005479 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVssDEEF.[-] N-Term(TMT6plex); S8(Phospho); S9(Phospho) 1 P16521 P16521 0 2 0.1463 0 1 1 925.35581 1849.70433 1849.70195 1.29 0.00119 668760.625 CID (Collision Induced Dissociation) MS2 0.0 30 58.6 60.0 139.303 47547 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 14.2 52.4 94.1 20.2 49.2 116.7 19.6 50.1 107.1 62.3 Excluded by Method 2.94 1 0.001796 0.005836 False High Sequest HT (A2) Unambiguous LTFDTTFSPNTGK [K].lTFDTTFsPNTGk.[K] N-Term(TMT6plex); S8(Phospho); K13(TMT6plex) 3 P45880 P45880; P45880-2; P45880-1 0 3 0.1921 0 1 1 656.33708 1966.99668 1966.99273 2.01 0.00132 1065732.625 CID (Collision Induced Dissociation) MS2 4.851918 40 112.8 60.0 112.4332 37395 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 125.2 113.4 95.1 121.9 94.9 106.0 132.6 123.0 115.6 100.1 Excluded by Method 4.06 1 0.001796 0.005267 False High Sequest HT (A2) Unambiguous SFDHLISDTK [K].sFDHLISDTk.[A] N-Term(TMT6plex); S1(Phospho); K10(TMT6plex) 3 P21359 P21359-2; P21359-6; P21359 0 2 0.188 0 1 1 850.93642 1700.86556 1700.86607 -0.3 -0.00025 2015111.5 CID (Collision Induced Dissociation) MS2 12.63167 50 40.3 60.0 94.3513 30587 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 49.0 39.9 36.8 37.7 39.5 40.3 38.3 42.1 41.8 38.1 Excluded by Method 2.5 1 0.001796 0.005296 False High Sequest HT (A2) Unambiguous YDSPVSSPITSASELGSIAK [K].yDsPVSSPITSASELGSIAk.[L] N-Term(TMT6plex); S3(Phospho); K20(TMT6plex) 1 P53890 P53890 0 3 0.0249 0 1 1 849.77178 2547.30077 2547.29954 0.48 0.00041 902225.625 CID (Collision Induced Dissociation) MS2 13.02807 40 33.9 60.0 120.5765 40332 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 12.6 30.7 48.3 9.5 28.4 62.3 18.9 30.0 62.4 35.4 Excluded by Method 2.41 1 0.001796 0.0056 False High Sequest HT (A2) Unambiguous LRENLNSSPTSEFK [K].lRENLNSsPTSEFk.[S] N-Term(TMT6plex); S8(Phospho); K14(TMT6plex) 1 P13382 P13382 1 3 0.0264 0 1 1 720.70859 2160.11122 2160.11022 0.46 0.00033 2394741.25 CID (Collision Induced Dissociation) MS2 0.0 60 70.3 48.494 81.0842 25655 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 25.3 65.1 109.8 22.7 52.5 125.8 28.7 60.9 135.9 76.2 Excluded by Method 2.65 1 0.001796 0.005536 False High Sequest HT (A2) Unambiguous NSEPSARHVDSLSQRSPK [K].nSEPSARHVDSLSQRsPk.[A] N-Term(TMT6plex); S16(Phospho); K18(TMT6plex) 4 Q9UHD8 Q9UHD8-7; Q9UHD8-2; Q9UHD8-5; Q9UHD8 2 4 0.117 0 1 1 634.07862 2533.29267 2533.29243 0.09 6e-05 993863.875 CID (Collision Induced Dissociation) MS2 13.3047 20 81.8 60.0 40.3108 10432 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 71.3 75.8 69.2 82.8 79.6 86.0 91.1 89.7 93.1 79.0 Excluded by Method 2.65 1 0.001796 0.005365 False High Sequest HT (A2) Unambiguous ENLANNEILDN [K].eNLANNEILDN.[-] N-Term(TMT6plex) 1 P35725 P35725 0 2 0.4266 0 1 1 744.38111 1487.75495 1487.75392 0.69 0.00052 623578.5 CID (Collision Induced Dissociation) MS2 45.76715 40 38.1 60.0 80.2879 25351 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 21.4 34.7 46.7 21.7 30.5 62.8 29.8 34.4 58.6 40.2 Excluded by Method 2.18 1 0.001796 0.005428 False High Sequest HT (A2) Unambiguous ISPDLNIK [K].isPDLNIk.[C] N-Term(TMT6plex); S2(Phospho); K8(TMT6plex) 1 Q69YH5 Q69YH5 0 2 0.2525 0 1 1 719.40903 1437.81079 1437.81185 -0.74 -0.00053 979135.75 CID (Collision Induced Dissociation) MS2 0.0 20 136.1 60.0 100.0665 32791 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 141.5 141.9 111.4 148.7 119.9 135.9 162.3 133.8 136.6 129.4 Excluded by Method 3.01 1 0.001796 0.006322 False High Sequest HT (A2) Unambiguous GSSERGSPIEK [K].gSSERGsPIEk.[Y] N-Term(TMT6plex); S7(Phospho); K11(TMT6plex) 3 Q9UHF7 Q9UHF7-3; Q9UHF7-2; Q9UHF7 1 3 0.2634 0 1 1 562.29378 1684.86679 1684.86714 -0.21 -0.00012 2951009.75 CID (Collision Induced Dissociation) MS2 2.761368 20 61.4 60.0 41.1636 10742 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 55.7 61.6 45.3 74.3 57.5 68.5 61.6 70.1 57.2 62.0 Excluded by Method 2.62 1 0.001796 0.006228 False High Sequest HT (A2) Unambiguous IPSLGWSPSK [K].iPSLGWsPSk.[H] N-Term(TMT6plex); S7(Phospho); K10(TMT6plex) 2 Q8IVF2 Q8IVF2-3; Q8IVF2 0 2 0.1392 0 1 1 805.4419 1609.87652 1609.87552 0.63 0.0005 1398649.25 CID (Collision Induced Dissociation) MS2 21.43542 50 76.1 60.0 116.5251 38905 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 86.6 76.6 65.8 67.7 67.2 76.2 87.2 82.8 78.8 72.4 Excluded by Method 2.73 1 0.001796 0.005337 False High Sequest HT (A2) Unambiguous KDDSHSAEDSEDEK [K].kDDSHsAEDsEDEk.[E] N-Term(TMT6plex); K1(TMT6plex); S6(Phospho); S10(Phospho); K14(TMT6plex) 2 Q9H1E3 Q9H1E3; Q9H1E3-2 1 3 0.4268 0 1 1 813.69097 2439.05835 2439.05689 0.6 0.00049 49906.6171875 CID (Collision Induced Dissociation) MS2 57.38243 40 279.1 60.0 39.6371 10188 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 288.0 271.6 192.8 345.4 262.7 236.5 356.3 287.2 288.6 262.2 Excluded by Method 4.92 1 0.001796 0.005235 False High Sequest HT (A2) Unambiguous SGLELTELQNMTVPEDDNISNDSNDFTEVENGQINSK [K].sGLELTELQNmTVPEDDNIsNDsNDFTEVENGQINSk.[F] N-Term(TMT6plex); M11(Oxidation); S20(Phospho); S23(Phospho); K37(TMT6plex) 1 Q9H2H9 Q9H2H9 0 4 0.2857 0 1 1 1183.53338 4731.11168 4731.09459 3.61 0.00427 788903.1875 CID (Collision Induced Dissociation) MS2 0.0 60 10.3 60.0 120.544 40319 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 6.4 12.0 8.8 14.3 8.5 9.5 9.2 14.2 11.1 8.6 Excluded by Method 4.9 1 0.001796 0.006357 False High Sequest HT (A2) Unambiguous QLELSINSASPNVNHSPVPTK [K].qLELSINSAsPNVNHsPVPTk.[K] N-Term(TMT6plex); S10(Phospho); S16(Phospho); K21(TMT6plex) 1 P32457 P32457 0 4 0.0399 0 1 1 713.36039 2850.41974 2850.42041 -0.23 -0.00017 232560.421875 CID (Collision Induced Dissociation) MS2 9.079239 30 27.7 60.0 99.5987 32618 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 8.3 22.8 43.8 9.1 22.7 48.0 10.6 28.0 55.2 28.8 Excluded by Method 3.76 1 0.001796 0.00604 False High Sequest HT (A2) Unambiguous GSMADVPK [K].gsMADVPk.[E] N-Term(TMT6plex); S2(Phospho); K8(TMT6plex) 2 P04806 P04806; P04807 0 2 0.5248 0 1 1 671.84596 1342.68465 1342.68421 0.33 0.00022 2346596.5 CID (Collision Induced Dissociation) MS2 0.0 60 67.7 53.922 66.3664 20067 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 24.3 56.4 85.1 33.0 51.5 130.3 38.9 61.2 128.5 68.1 Excluded by Method 2.82 1 0.001796 0.005952 False High Sequest HT (A2) Unambiguous SPFSVAVSPSLDLSK [K].sPFSVAVsPSLDLSk.[I] N-Term(TMT6plex); S8(Phospho); K15(TMT6plex) 2 P21333 P21333; P21333-2 0 3 0.0404 0 1 1 691.37356 2072.10614 2072.10809 -0.94 -0.00065 438181.8125 CID (Collision Induced Dissociation) MS2 0.0 80 51.8 60.0 126.1784 42389 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 53.1 56.4 46.0 43.6 56.2 55.4 47.0 55.2 55.1 49.8 4.21 1 0.001796 0.005416 False High Sequest HT (A2) Unambiguous DVLDIEQFSTVK [K].dVLDIEQFstVk.[G] N-Term(TMT6plex); S9(Phospho); T10(Phospho); K12(TMT6plex) 4 P34947 P34947; P43250; P43250-2; P43250-3 0 3 0.391 0 1 1 671.33155 2011.98009 2011.97946 0.31 0.00021 328184.09375 CID (Collision Induced Dissociation) MS2 64.97319 60 48.8 60.0 125.1754 42013 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 39.2 43.1 45.0 49.2 42.7 60.0 48.6 54.5 62.5 42.9 Excluded by Method 2.66 1 0.001796 0.005356 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVSsDEEF.[-] N-Term(TMT6plex); S9(Phospho) 1 P16521 P16521 0 2 0.056 0 1 1 885.37065 1769.73402 1769.73562 -0.91 -0.0008 470370.8125 CID (Collision Induced Dissociation) MS2 0.0 40 52.1 60.0 120.0758 40142 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 13.3 38.7 76.1 15.9 44.9 107.5 20.4 46.7 95.8 62.1 Excluded by Method 2.68 1 0.001796 0.005461 False High Sequest HT (A2) Unambiguous HIVSNDSSDSDDESHEPK [K].hIVSNDSSDsDDESHEPk.[G] N-Term(TMT6plex); S10(Phospho); K18(TMT6plex) 1 P47712 P47712 0 4 0.1333 0 1 1 634.78644 2536.12393 2536.12409 -0.06 -4e-05 296084.5625 CID (Collision Induced Dissociation) MS2 3.988275 10 28.6 60.0 33.8675 8166 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 32.3 26.7 25.0 31.4 32.9 21.4 43.3 29.0 13.8 30.0 Excluded by Method 2.55 1 0.001796 0.005426 False High Sequest HT (A2) Unambiguous ASAPERTPSPAPK [K].aSAPERtPsPAPk.[R] N-Term(TMT6plex); T7(Phospho); S9(Phospho); K13(TMT6plex) 4 Q63ZY6 Q63ZY6-6; Q63ZY6; Q96P11-2; Q3KNT7-3 1 3 0.2445 0 1 1 642.98809 1926.94972 1926.94917 0.29 0.00019 1594351.5 CID (Collision Induced Dissociation) MS2 2.274644 10 189.6 58.079 46.2511 12627 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 189.2 189.6 160.0 192.1 180.7 189.3 230.0 201.5 183.9 179.3 Excluded by Method 4.09 1 0.001796 0.005737 False High Sequest HT (A2) Unambiguous NKPGPNIESGNEDDDASFK [K].nkPGPNIEsGNEDDDASFk.[I] N-Term(TMT6plex); K2(TMT6plex); S9(Phospho); K19(TMT6plex) 1 O60841 O60841 0 5 0.3333 0 1 1 561.07796 2801.36071 2801.35979 0.33 0.00018 748003.6875 CID (Collision Induced Dissociation) MS2 22.53379 40 55.2 60.0 76.5189 23857 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 50.2 59.7 45.6 51.3 50.4 64.8 61.7 58.5 54.5 55.2 Excluded by Method 3.24 1 0.001796 0.005986 False High Sequest HT (A2) Unambiguous EEPPPARSGSGGGSAK [K].eEPPPARSGsGGGSAk.[E] N-Term(TMT6plex); S10(Phospho); K16(TMT6plex) 5 Q9H4A3 Q9H4A3-5; Q9H4A3-2; Q9H4A3-7; Q9H4A3; Q9H4A3-6 1 3 0.0551 0 1 1 674.67328 2022.00528 2022.00575 -0.24 -0.00016 118555.984375 CID (Collision Induced Dissociation) MS2 8.808425 30 70.7 60.0 36.8746 9188 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 78.2 71.5 55.7 79.6 63.2 57.7 76.2 82.9 70.8 71.3 Excluded by Method 2.36 1 0.001796 0.00548 False High Sequest HT (A2) Unambiguous TSTSPPPEK [K].tSTsPPPEk.[S] N-Term(TMT6plex); S4(Phospho); K9(TMT6plex) 2 Q9H1E3 Q9H1E3; Q9H1E3-2 0 3 0.0138 0 1 1 494.59344 1481.76577 1481.7653 0.32 0.00016 2528743.5 CID (Collision Induced Dissociation) MS2 7.146666 40 183.4 60.0 45.5764 12379 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 187.9 182.6 149.9 183.0 176.7 183.5 207.1 189.8 182.2 191.1 Excluded by Method 2.17 1 0.001953 0.006634 False High Sequest HT (A2) Unambiguous ALEAELDAQHLSETFDNIDNLSPK [K].aLEAELDAQHLSETFDNIDNLsPk.[A] N-Term(TMT6plex); S22(Phospho); K24(TMT6plex) 3 P25054 P25054-3; P25054; P25054-2 0 4 0.0291 0 1 1 802.90052 3208.58025 3208.58152 -0.4 -0.00032 565470.8125 CID (Collision Induced Dissociation) MS2 15.58476 20 42.6 60.0 126.0868 42429 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 43.8 52.9 31.1 33.5 38.3 48.5 40.9 48.6 45.3 43.4 Excluded by Method 4.13 1 0.001953 0.006747 False High Sequest HT (A2) Unambiguous YAPVSPTVQQK [K].yAPVSPTVQQk.[Q] N-Term(TMT6plex); Y1(Phospho); K11(TMT6plex) 1 P48415 P48415 0 3 0.0062 0 1 1 585.98624 1755.94418 1755.94466 -0.28 -0.00016 2405136.0 CID (Collision Induced Dissociation) MS2 11.8018 40 139.1 60.0 72.4543 22324 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 110.5 119.8 145.7 121.2 136.8 170.3 134.3 149.1 161.1 141.9 Excluded by Method 3.21 1 0.001953 0.006728 False High Sequest HT (A2) Unambiguous EFGILK [K].eFGILk.[K] N-Term(TMT6plex); K6(TMT6plex) 1 P00549 P00549 0 2 0.3095 0 1 1 582.87339 1164.73951 1164.73927 0.21 0.00012 774285.5625 CID (Collision Induced Dissociation) MS2 0.0 60 51.7 60.0 93.5839 30264 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 21.5 54.8 60.2 20.6 40.3 74.1 41.6 62.6 79.5 61.7 Excluded by Method 2.1 1 0.001953 0.006774 False High Sequest HT (A2) Unambiguous EVPSKEEPSPVK [K].eVPSkEEPsPVk.[A] N-Term(TMT6plex); K5(TMT6plex); S9(Phospho); K12(TMT6plex) 1 P46821 P46821 1 3 0.3442 0 1 1 698.3881 2093.14975 2093.14985 -0.05 -3e-05 877856.75 CID (Collision Induced Dissociation) MS2 3.99353 30 104.9 60.0 63.7711 19104 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 105.6 102.5 81.7 117.4 103.7 98.4 115.9 112.2 112.0 99.2 Excluded by Method 2.76 1 0.001953 0.006821 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVssDEEF.[-] N-Term(TMT6plex); S8(Phospho); S9(Phospho) 1 P16521 P16521 0 2 0.1626 0 1 1 925.35577 1849.70427 1849.70195 1.25 0.00116 350139.8125 CID (Collision Induced Dissociation) MS2 14.2043 40 55.4 60.0 148.1109 50549 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 12.3 50.0 92.8 16.5 35.0 106.3 21.2 50.9 111.4 57.5 Excluded by Method 3.26 1 0.001953 0.006626 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVssDEEF.[-] N-Term(TMT6plex); S8(Phospho); S9(Phospho) 1 P16521 P16521 0 2 0.1715 0 1 1 925.3549 1849.70252 1849.70195 0.31 0.00028 541792.0625 CID (Collision Induced Dissociation) MS2 43.25547 50 68.4 60.0 147.7362 50434 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 16.9 51.5 121.9 22.4 57.1 122.3 25.7 66.7 130.1 69.1 Excluded by Method 2.74 1 0.001953 0.006754 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVsSDEEF.[-] N-Term(TMT6plex); S8(Phospho) 1 P16521 P16521 0 2 0.0472 0 1 1 885.37144 1769.73561 1769.73562 -0.01 -1e-05 353921.15625 CID (Collision Induced Dissociation) MS2 20.58621 40 56.9 60.0 135.594 46251 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 17.1 46.0 85.6 21.7 51.3 114.3 16.6 57.8 105.1 53.5 Excluded by Method 3.18 1 0.001953 0.006737 False High Sequest HT (A2) Unambiguous EEVQELEELNK [K].eEVQELEELNk.[T] N-Term(TMT6plex); K11(TMT6plex) 1 P15108 P15108 0 3 0.0 0 1 1 606.66888 1817.9921 1817.98968 1.33 0.00081 1337394.625 CID (Collision Induced Dissociation) MS2 29.20187 70 3.5 60.0 93.8841 30376 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 3.0 1.4 3.0 1.6 2.8 8.0 2.3 1.8 7.6 3.4 0.92 1 0.001953 0.006722 False High Sequest HT (A2) Unambiguous HLDVDLDRQSLSSIDK [K].hLDVDLDRQSLssIDk.[N] N-Term(TMT6plex); S12(Phospho); S13(Phospho); K16(TMT6plex) 1 Q9Y6D5 Q9Y6D5 1 3 0.0725 0 1 1 820.40459 2459.19922 2459.19846 0.31 0.00025 758748.1875 CID (Collision Induced Dissociation) MS2 0.0 40 50.1 60.0 94.6704 30755 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 33.5 50.5 48.7 49.0 39.7 59.9 48.0 59.8 64.6 46.8 Excluded by Method 2.07 1 0.001953 0.006733 False High Sequest HT (A2) Unambiguous EEEAKEESDDDMGFGLFD [K].eEEAkEEsDDDmGFGLFD.[-] N-Term(TMT6plex); K5(TMT6plex); S8(Phospho); M12(Oxidation) 1 P02400 P02400 1 2 0.5401 0 1 1 1309.05208 2617.09688 2617.0941 1.06 0.00139 788850.125 CID (Collision Induced Dissociation) MS2 0.0 20 84.9 60.0 112.8918 37533 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 16.0 71.7 126.2 27.1 68.2 166.0 33.3 72.6 188.0 79.7 Excluded by Method 2.87 1 0.001953 0.006697 False High Sequest HT (A2) Unambiguous ASGRGAESPFEEK [K].aSGRGAEsPFEEk.[S] N-Term(TMT6plex); S8(Phospho); K13(TMT6plex) 1 P46821 P46821 1 3 0.2414 0 1 1 634.98347 1902.93585 1902.93628 -0.22 -0.00014 2339661.5 CID (Collision Induced Dissociation) MS2 11.88211 30 144.2 56.331 56.518 16379 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 138.0 129.8 119.2 151.5 133.9 147.4 179.5 147.1 143.9 152.0 Excluded by Method 2.32 1 0.001953 0.006743 False High Sequest HT (A2) Unambiguous DAQGQPGLERAQQELVPPQQQASPPQLPK [K].dAQGQPGLERAQQELVPPQQQAsPPQLPk.[A] N-Term(TMT6plex); S23(Phospho); K29(TMT6plex) 5 O15027 O15027-5; O15027-4; O15027; O15027-2; O15027-3 1 5 0.1333 0 1 1 735.58825 3673.91213 3673.91034 0.49 0.00036 1039341.9375 CID (Collision Induced Dissociation) MS2 9.148199 30 98.4 51.437 96.2052 31284 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 98.0 101.9 83.4 100.8 96.5 99.4 126.1 102.4 87.8 88.1 Excluded by Method 2.4 1 0.001953 0.006781 False High Sequest HT (A2) Unambiguous KPQARKGSMADVPK [K].kPQARkGsmADVPk.[E] N-Term(TMT6plex); K1(TMT6plex); K6(TMT6plex); S8(Phospho); M9(Oxidation); K14(TMT6plex) 2 P04806 P04806; P04807 2 5 0.3086 0 1 1 505.89477 2525.44474 2525.44448 0.1 5e-05 945626.5 CID (Collision Induced Dissociation) MS2 0.0 20 44.3 60.0 47.5584 13100 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 16.8 40.9 56.5 17.3 43.4 75.1 17.5 48.8 69.3 57.0 Excluded by Method 3.37 1 0.001953 0.006553 False High Sequest HT (A2) Unambiguous LFEESDDK [K].lFEEsDDk.[E] N-Term(TMT6plex); S5(Phospho); K8(TMT6plex) 1 O43719 O43719 0 3 0.248 0 1 1 507.58098 1520.72838 1520.72858 -0.13 -7e-05 1028972.0625 CID (Collision Induced Dissociation) MS2 0.0 10 41.7 60.0 87.7111 28146 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 28.9 33.7 40.2 42.5 38.1 52.7 38.1 45.6 59.9 37.2 Excluded by Method 2.5 1 0.001953 0.006677 False High Sequest HT (A2) Unambiguous FYDDENLTE [K].fYDDENLtE.[-] N-Term(TMT6plex); T8(Phospho) 1 P50278 P50278 0 2 0.6698 0 1 1 727.80013 1454.59299 1454.59259 0.28 0.0002 38378864.0 CID (Collision Induced Dissociation) MS2 37.68016 40 137.7 3.061 94.7749 30714 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 61.9 118.9 198.4 61.6 108.6 242.2 71.4 130.4 235.8 148.2 Excluded by Method 3.24 1 0.001953 0.006684 False High Sequest HT (A2) Unambiguous ETTYDEIK [K].eTTYDEIk.[K] N-Term(TMT6plex); K8(TMT6plex) 2 P00359 P00359; P00358 0 2 0.3216 0 1 1 728.90009 1456.79289 1456.79355 -0.45 -0.00033 1582158.375 CID (Collision Induced Dissociation) MS2 0.0 40 114.4 60.0 62.7181 18710 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 53.2 96.3 142.2 64.5 105.0 183.9 62.0 111.6 201.8 123.4 Excluded by Method 2.55 1 0.001953 0.00647 False High Sequest HT (A2) Unambiguous STSAPQMSPGSSDNQSSSPQPAQQK [K].sTSAPQmSPGSSDNQsSSPQPAQQk.[L] N-Term(TMT6plex); M7(Oxidation); S16(Phospho); K25(TMT6plex) 5 Q14157-1 Q14157-1; Q14157-5; Q14157-4; Q14157; Q14157-3 0 4 0.0031 0 1 1 772.35871 3086.413 3086.41381 -0.26 -0.0002 101741.7265625 CID (Collision Induced Dissociation) MS2 21.93839 50 21.8 60.0 39.0673 10035 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 20.0 23.5 15.2 21.9 19.2 23.4 26.5 25.0 21.6 21.8 Excluded by Method 3.25 1 0.001953 0.006589 False High Sequest HT (A2) Unambiguous TDEERPPVEHSPEK [K].tDEERPPVEHsPEk.[Q] N-Term(TMT6plex); S11(Phospho); K14(TMT6plex) 1 Q15170 Q15170 0 4 0.4922 0 1 1 547.77348 2188.0721 2188.06875 1.53 0.00084 333698.90625 CID (Collision Induced Dissociation) MS2 3.674956 40 105.1 60.0 44.4154 11916 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 101.2 97.5 84.3 124.7 102.9 100.6 111.6 111.1 110.4 106.8 Excluded by Method 3.84 1 0.001953 0.006816 False High Sequest HT (A2) Unambiguous VQGEAVSNIQENTQTPTVQEESEEEEVDETGVEVK [K].vQGEAVSNIQENTQTPtVQEESEEEEVDETGVEVk.[D] N-Term(TMT6plex); T17(Phospho); K35(TMT6plex) 3 Q13765 Q13765; E9PAV3; E9PAV3-2 0 4 0.05 0 1 1 1100.51886 4399.05361 4399.06016 -1.49 -0.00164 161695.390625 CID (Collision Induced Dissociation) MS2 17.67084 70 6.9 60.0 146.3005 49927 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 6.8 4.1 3.1 6.3 3.8 8.0 8.8 11.1 9.5 7.1 3.0 1 0.001953 0.006457 False High Sequest HT (A2) Unambiguous QGSPDQVSPVSEMTSTSLYQDK [K].qGSPDQVsPVSEMTSTSLYQDk.[Q] N-Term(TMT6plex); S8(Phospho); K22(TMT6plex) 1 P46821 P46821 0 3 0.1343 0 1 1 974.80067 2922.38745 2922.38441 1.04 0.00102 420871.75 CID (Collision Induced Dissociation) MS2 0.0 60 10.0 60.0 106.7849 35292 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 6.5 7.2 11.0 6.6 10.1 15.8 12.0 8.2 10.5 11.6 Excluded by Method 2.16 1 0.001953 0.006534 False High Sequest HT (A2) Unambiguous DAQRLSPIPEEVPK [K].dAQRLsPIPEEVPk.[S] N-Term(TMT6plex); S6(Phospho); K14(TMT6plex) 3 Q96T23 Q96T23; Q96T23-3; Q96T23-2 1 3 0.4812 0 1 1 706.38507 2117.14066 2117.14079 -0.06 -4e-05 3692092.75 CID (Collision Induced Dissociation) MS2 1.781654 30 129.2 37.17 83.0202 26296 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 132.5 117.5 106.3 134.8 123.0 130.2 142.9 141.7 131.7 131.9 Excluded by Method 3.45 1 0.001953 0.00669 False High Sequest HT (A2) Unambiguous LSGFSFK [K].lSGFsFk.[K] N-Term(TMT6plex); S5(Phospho); K7(TMT6plex) 1 P29966 P29966 0 2 0.3781 0 1 1 662.3594 1323.71153 1323.71141 0.09 6e-05 3293324.0 CID (Collision Induced Dissociation) MS2 0.0 10 209.5 55.24 111.6518 37070 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 232.6 230.7 178.1 231.5 213.1 178.8 256.2 211.8 180.8 181.8 Excluded by Method 2.83 1 0.001953 0.006781 False High Sequest HT (A2) Unambiguous NGVAAEVSPAK [K].nGVAAEVsPAk.[E] N-Term(TMT6plex); S8(Phospho); K11(TMT6plex) 1 Q9BW71 Q9BW71 0 3 0.4506 0 1 1 527.62008 1580.84569 1580.84494 0.47 0.00025 661597.875 CID (Collision Induced Dissociation) MS2 0.0 70 9.7 60.0 58.7513 17223 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 7.5 8.7 7.2 9.8 6.8 11.7 10.9 12.5 9.2 13.2 2.53 1 0.001953 0.006452 False High Sequest HT (A2) Unambiguous QTELFVTDNSSK [K].qTELFVTDNsSk.[I] N-Term(TMT6plex); S10(Phospho); K12(TMT6plex) 1 Q06315 Q06315 0 3 0.0823 0 1 1 636.3238 1906.95684 1906.95635 0.26 0.00017 902516.875 CID (Collision Induced Dissociation) MS2 3.326567 40 20.3 60.0 88.6829 28429 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 10.7 18.2 25.8 12.4 15.4 30.5 16.1 20.2 31.8 21.6 Excluded by Method 2.43 1 0.001953 0.006712 False High Sequest HT (A2) Unambiguous SFTNIEQDYK [K].sFTNIEQDYk.[Q] N-Term(TMT6plex); S1(Phospho); K10(TMT6plex) 1 Q07528 Q07528 0 3 0.0115 0 1 1 594.96185 1782.87099 1782.87155 -0.31 -0.00019 228711.5 CID (Collision Induced Dissociation) MS2 65.1134 70 89.5 60.0 95.8257 31142 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 28.3 70.8 126.8 32.3 70.0 168.5 37.1 82.0 176.6 103.0 3.49 1 0.002055 0.008212 False High Sequest HT (A2) Unambiguous DISASSPNLGGILK [K].dIsASSPNLGGILk.[A] N-Term(TMT6plex); S3(Phospho); K14(TMT6plex) 1 Q08548 Q08548 0 3 0.0292 0 1 1 637.35144 1910.03977 1910.04002 -0.13 -8e-05 591347.625 CID (Collision Induced Dissociation) MS2 31.35767 70 76.3 60.0 116.527 38906 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 23.5 61.2 108.8 25.5 65.2 145.6 27.9 72.6 150.5 82.4 3.08 1 0.002055 0.008122 False High Sequest HT (A2) Unambiguous GDDDEESDEEAVKK [K].gDDDEEsDEEAVkk.[T] N-Term(TMT6plex); S7(Phospho); K13(TMT6plex); K14(TMT6plex) 1 O43395 O43395 1 3 0.454 0 1 1 778.3721 2333.10176 2333.10006 0.73 0.00057 688953.8125 CID (Collision Induced Dissociation) MS2 14.1508 20 39.9 60.0 56.7302 16510 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 41.0 37.2 32.9 43.9 40.7 41.5 42.4 43.8 33.6 41.5 Excluded by Method 3.48 1 0.002055 0.00712 False High Sequest HT (A2) Unambiguous EEPSPVK [K].eEPsPVk.[A] N-Term(TMT6plex); S4(Phospho); K7(TMT6plex) 1 P46821 P46821 0 2 0.3182 0 1 1 662.35202 1323.69676 1323.69616 0.46 0.0003 14942366.0 CID (Collision Induced Dissociation) MS2 2.42121 40 207.9 27.475 50.155 14046 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 204.0 198.6 152.9 209.4 199.8 212.5 238.4 242.6 206.3 214.5 Excluded by Method 2.86 1 0.002055 0.008292 False High Sequest HT (A2) Unambiguous EEEAKEESDDDMGFGLFD [K].eEEAkEEsDDDmGFGLFD.[-] N-Term(TMT6plex); K5(TMT6plex); S8(Phospho); M12(Oxidation) 1 P02400 P02400 1 3 0.6845 0 1 1 873.03747 2617.09786 2617.0941 1.44 0.00125 1557855.125 CID (Collision Induced Dissociation) MS2 9.701044 30 137.0 49.929 124.2525 41754 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 37.2 115.1 206.0 43.7 107.1 254.0 48.4 123.9 297.6 136.5 Excluded by Method 3.36 1 0.002055 0.007504 False High Sequest HT (A2) Unambiguous VEPVEGSLQSK [K].vEPVEGsLQSk.[L] N-Term(TMT6plex); S7(Phospho); K11(TMT6plex) 1 P38845 P38845 0 3 0.3364 0 1 1 570.97412 1710.90782 1710.90794 -0.07 -4e-05 852399.25 CID (Collision Induced Dissociation) MS2 0.0 60 51.5 60.0 75.0616 23303 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 19.0 40.0 68.5 18.2 51.6 92.0 20.5 48.7 96.7 59.5 Excluded by Method 3.21 1 0.002055 0.007318 False High Sequest HT (A2) Unambiguous TNAFDFGSSSFGSGFSK [K].tNAFDFGSSsFGSGFSk.[A] N-Term(TMT6plex); S10(Phospho); K17(TMT6plex) 1 P40477 P40477 0 3 0.016 0 1 1 761.02386 2281.05701 2281.05785 -0.37 -0.00028 485745.59375 CID (Collision Induced Dissociation) MS2 31.3299 30 24.5 60.0 124.5634 41866 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 11.7 19.6 34.5 9.1 18.0 45.4 14.3 23.3 47.7 21.8 Excluded by Method 2.5 1 0.002055 0.008557 False High Sequest HT (A2) Unambiguous SRSIEEK [K].sRsIEEk.[D] N-Term(TMT6plex); S3(Phospho); K7(TMT6plex) 1 Q04279 Q04279 1 3 0.251 0 1 1 462.91763 1386.73835 1386.73942 -0.77 -0.00036 548343.25 CID (Collision Induced Dissociation) MS2 11.91651 50 12.3 60.0 38.522 9834 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 3.7 6.7 21.3 3.2 12.3 20.8 2.2 12.6 25.8 14.1 Excluded by Method 2.39 1 0.002055 0.006941 False High Sequest HT (A2) Unambiguous ERIVRAFLIEEQK [K].eRIVRAFLIEEQk.[I] N-Term(TMT6plex); K13(TMT6plex) 1 P40525 P40525 2 4 0.1038 0 1 1 523.06891 2089.2538 2089.25338 0.2 0.0001 228156.359375 CID (Collision Induced Dissociation) MS2 63.47704 60 11.3 60.0 93.1354 30182 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 2.2 10.0 15.9 8.3 8.3 16.6 9.0 13.0 19.1 10.1 Excluded by Method 2.12 1 0.002055 0.007808 False High Sequest HT (A2) Unambiguous LSDDFDSPVK [K].lSDDFDsPVk.[G] N-Term(TMT6plex); S7(Phospho); K10(TMT6plex) 3 Q96T23 Q96T23; Q96T23-3; Q96T23-2 0 2 0.4 0 1 1 830.916 1660.82472 1660.82354 0.71 0.00059 4679959.5 CID (Collision Induced Dissociation) MS2 61.2876 50 124.9 26.255 90.808 29254 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 118.9 128.0 102.3 132.0 117.6 134.9 125.8 131.1 134.5 124.1 Excluded by Method 3.55 1 0.002055 0.00711 False High Sequest HT (A2) Unambiguous KPQARKGSMADVPK [K].kPQARkGsMADVPk.[E] N-Term(TMT6plex); K1(TMT6plex); K6(TMT6plex); S8(Phospho); K14(TMT6plex) 2 P04806 P04806; P04807 2 5 0.325 0 1 1 502.69577 2509.44974 2509.44957 0.07 3e-05 357917.375 CID (Collision Induced Dissociation) MS2 57.01979 30 56.4 60.0 55.5998 16063 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 31.1 51.6 63.7 30.3 53.2 87.8 36.1 56.7 91.8 61.5 Excluded by Method 2.4 1 0.002055 0.007277 False High Sequest HT (A2) Unambiguous LEAGLSDSK [K].lEAGLsDSk.[Q] N-Term(TMT6plex); S6(Phospho); K9(TMT6plex) 1 P17536 P17536 0 2 0.3176 0 1 1 729.38698 1457.76669 1457.7653 0.96 0.0007 1064295.0 CID (Collision Induced Dissociation) MS2 39.16224 40 74.0 60.0 77.8077 24340 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 23.4 65.4 94.4 28.4 59.5 142.5 34.7 66.1 138.9 86.5 Excluded by Method 3.18 1 0.002055 0.007743 False High Sequest HT (A2) Unambiguous LTNRLDDLLERSQELTISK [K].lTNRLDDLLERSQELtISk.[E] N-Term(TMT6plex); T16(Phospho); K19(TMT6plex) 1 P38853 P38853 2 4 0.0349 0 1 1 696.38403 2782.5143 2782.5116 0.97 0.00068 1055371.75 CID (Collision Induced Dissociation) MS2 6.149477 30 43.7 60.0 122.7385 41198 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 12.4 38.0 64.9 11.5 33.5 78.6 21.5 37.7 95.8 42.7 Excluded by Method 4.01 1 0.002055 0.007239 False High Sequest HT (A2) Unambiguous VVAETTYIDTPDTETK [K].vVAETTYIDtPDTETk.[K] N-Term(TMT6plex); T10(Phospho); K16(TMT6plex) 1 Q08412 Q08412 0 3 0.1215 0 1 1 774.39081 2321.15787 2321.15656 0.56 0.00044 412697.1875 CID (Collision Induced Dissociation) MS2 64.0831 0 52.2 60.0 89.0972 28605 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 50.9 58.2 50.2 44.1 51.7 54.7 49.9 52.8 55.1 53.9 Excluded by Method 4.28 1 0.002055 0.008095 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVSsDEEF.[-] N-Term(TMT6plex); S9(Phospho) 1 P16521 P16521 0 2 0.0127 0 1 1 885.37138 1769.73548 1769.73562 -0.08 -7e-05 229359.125 CID (Collision Induced Dissociation) MS2 41.88658 50 35.4 60.0 137.866 47045 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 9.4 25.3 59.5 9.6 27.1 68.9 14.5 30.8 78.3 30.2 Excluded by Method 2.37 1 0.002055 0.008375 False High Sequest HT (A2) Unambiguous ALEEVSLK [K].aLEEVsLk.[W] N-Term(TMT6plex); S6(Phospho); K8(TMT6plex) 1 P14126 P14126 0 2 0.263 0 1 1 713.90212 1426.79696 1426.79587 0.77 0.00055 1448603.0 CID (Collision Induced Dissociation) MS2 24.46467 20 42.4 60.0 90.0806 28977 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 12.4 35.9 61.1 19.0 30.0 81.8 19.1 38.1 82.0 45.0 Excluded by Method 3.27 1 0.002055 0.00776 False High Sequest HT (A2) Unambiguous IDFRVYNLLTPDSK [K].iDFRVYNLLtPDSk.[M] N-Term(TMT6plex); T10(Phospho); K14(TMT6plex) 3 Q9UHF7 Q9UHF7-3; Q9UHF7-2; Q9UHF7 1 3 0.1926 0 1 1 740.40125 2219.18918 2219.18774 0.65 0.00048 539746.375 CID (Collision Induced Dissociation) MS2 49.93444 60 41.9 60.0 125.0738 41977 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 43.3 41.6 35.5 38.2 37.2 42.5 49.1 47.6 44.1 39.7 Excluded by Method 2.7 1 0.002055 0.007663 False High Sequest HT (A2) Unambiguous ELEAERSLMSSPEDLTK [K].eLEAERSLmssPEDLTk.[D] N-Term(TMT6plex); M9(Oxidation); S10(Phospho); S11(Phospho); K17(TMT6plex) 1 P46821 P46821 1 3 0.0775 0 1 1 857.0691 2569.19276 2569.19099 0.69 0.00059 969744.9375 CID (Collision Induced Dissociation) MS2 17.43467 40 92.5 60.0 91.3137 29447 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 89.0 96.5 72.5 100.8 87.4 92.4 101.7 93.9 94.8 96.1 Excluded by Method 2.58 1 0.002055 0.008289 False High Sequest HT (A2) Unambiguous EMEHNTVCAAGTSPVGEIGEEK [K].emEHNTVcAAGTsPVGEIGEEk.[I] N-Term(TMT6plex); M2(Oxidation); C8(Carbamidomethyl); S13(Phospho); K22(TMT6plex) 9 P18583 P18583; P18583-4; P18583-6; P18583-5; P18583-10; P18583-9; P18583-2; P18583-3; P18583-7 0 3 0.0382 0 1 1 967.11373 2899.32662 2899.32551 0.38 0.00037 1087950.0 CID (Collision Induced Dissociation) MS2 25.71844 20 146.7 60.0 72.4676 22329 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 159.3 143.8 116.3 173.1 148.1 133.2 175.6 145.0 132.4 140.3 Excluded by Method 3.14 1 0.002055 0.008204 False High Sequest HT (A2) Unambiguous DSIINSPVSGRQSISPTLSNATTTTTK [K].dSIINSPVSGRQsIsPTLSNATTTTTk.[S] N-Term(TMT6plex); S13(Phospho); S15(Phospho); K27(TMT6plex) 1 P36024 P36024 1 4 0.053 0 1 1 849.42867 3394.69286 3394.69083 0.6 0.00051 1353079.875 CID (Collision Induced Dissociation) MS2 38.94395 60 34.4 54.252 98.8074 32262 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 12.5 30.2 56.8 13.1 29.0 58.2 14.7 35.8 57.6 36.0 Excluded by Method 3.02 1 0.002055 0.007079 False High Sequest HT (A2) Unambiguous EQAEASIDNLK [K].eQAEAsIDNLk.[N] N-Term(TMT6plex); S6(Phospho); K11(TMT6plex) 1 Q08245 Q08245 0 3 0.3248 0 1 1 585.96941 1755.89369 1755.89302 0.38 0.00022 1015168.125 CID (Collision Induced Dissociation) MS2 13.51209 50 25.3 60.0 71.0521 21881 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 11.7 20.0 39.2 14.6 17.0 44.2 12.1 23.0 46.9 24.7 Excluded by Method 2.34 1 0.002055 0.006918 False High Sequest HT (A2) Unambiguous SLPENSTASAASAFDK [K].sLPENStASAASAFDk.[A] N-Term(TMT6plex); T7(Phospho); K16(TMT6plex) 1 P41832 P41832 0 3 0.0152 0 1 1 712.01998 2134.04539 2134.04695 -0.73 -0.00052 1227001.0 CID (Collision Induced Dissociation) MS2 5.710379 50 107.9 60.0 92.7836 29997 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 27.3 75.5 164.9 40.9 100.1 196.1 36.6 100.3 220.5 116.5 Excluded by Method 3.95 1 0.002055 0.007229 False High Sequest HT (A2) Unambiguous FGSADNIPNLK [K].fGsADNIPNLk.[D] N-Term(TMT6plex); S3(Phospho); K11(TMT6plex) 2 O94876 O94876-2; O94876 0 2 0.4777 0 1 1 857.45307 1713.89887 1713.89771 0.68 0.00058 1215217.75 CID (Collision Induced Dissociation) MS2 12.6275 40 83.6 50.229 105.025 34597 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 69.6 75.3 83.9 76.8 72.6 113.7 73.3 84.4 105.7 80.9 Excluded by Method 3.81 1 0.002055 0.007897 False High Sequest HT (A2) Unambiguous QAIYTRATASE [K].qAIYTRATAsE.[-] N-Term(TMT6plex); S10(Phospho) 1 Q3E792 Q3E792 1 2 0.1589 0 1 1 760.37139 1519.7355 1519.7355 0.0 0.0 2007967.625 CID (Collision Induced Dissociation) MS2 16.16669 20 41.0 60.0 54.1181 15536 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 11.4 34.3 65.0 17.7 35.4 69.6 17.1 34.2 77.9 46.9 Excluded by Method 2.14 1 0.002055 0.00731 False High Sequest HT (A2) Unambiguous DMSPLSETEMALGK [K].dmsPLSETEmALGk.[D] N-Term(TMT6plex); M2(Oxidation); S3(Phospho); M10(Oxidation); K14(TMT6plex) 3 P27816 P27816; P27816-6; P27816-2 0 3 0.0889 0 1 1 693.66504 2078.98057 2078.97913 0.69 0.00048 956896.1875 CID (Collision Induced Dissociation) MS2 14.82921 50 59.1 60.0 111.1349 36907 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 57.5 57.9 56.4 51.9 56.2 63.7 55.7 59.1 73.6 58.8 Excluded by Method 2.7 1 0.002055 0.007488 False High Sequest HT (A2) Unambiguous RIACEEEFSDSEEEGEGGRK [K].rIAcEEEFsDsEEEGEGGRk.[N] N-Term(TMT6plex); C4(Carbamidomethyl); S9(Phospho); S11(Phospho); K20(TMT6plex) 1 Q13547 Q13547 2 4 0.3042 0 1 1 733.81758 2932.24849 2932.24733 0.39 0.00029 1054141.5 CID (Collision Induced Dissociation) MS2 21.17222 30 77.0 60.0 63.7214 19067 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 81.4 68.5 60.3 72.4 76.6 81.2 95.6 80.6 77.7 75.4 Excluded by Method 3.09 1 0.002055 0.008348 False High Sequest HT (A2) Unambiguous TEVPGSPAGTEGNCQEATGPSTVDTQNEPLDMK [K].tEVPGsPAGTEGNcQEATGPSTVDTQNEPLDmk.[E] N-Term(TMT6plex); S6(Phospho); C14(Carbamidomethyl); M32(Oxidation); K33(TMT6plex) 3 Q32MZ4 Q32MZ4-2; Q32MZ4; Q32MZ4-3 0 4 0.2118 0 1 1 993.70244 3971.78794 3971.79279 -1.22 -0.00121 131412.015625 CID (Collision Induced Dissociation) MS2 0.0 50 25.6 60.0 78.0701 24524 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 28.3 22.5 23.4 25.9 26.1 23.7 26.7 26.6 26.6 26.4 Excluded by Method 3.4 1 0.002055 0.007959 False High Sequest HT (A2) Unambiguous ELSPAGSISK [K].eLsPAGsISk.[N] N-Term(TMT6plex); S3(Phospho); S7(Phospho); K10(TMT6plex) 4 O95453 O95453-4; O95453-2; O95453-3; O95453 0 3 0.3564 0 1 1 536.26784 1606.78896 1606.78948 -0.32 -0.00017 1338875.125 CID (Collision Induced Dissociation) MS2 12.75355 40 44.5 60.0 77.3506 24202 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 41.2 40.4 39.5 41.0 40.2 45.6 52.3 51.3 44.0 49.0 Excluded by Method 3.03 1 0.002055 0.007768 False High Sequest HT (A2) Unambiguous SLCRTGSCSELDACPSK [K].sLcRTGscSELDAcPSk.[I] N-Term(TMT6plex); C3(Carbamidomethyl); S7(Phospho); C8(Carbamidomethyl); C14(Carbamidomethyl); K17(TMT6plex) 4 Q9H1H9 Q9H1H9-2; Q9H1H9; Q9H1H9-3; Q9H1H9-4 1 3 0.0633 0 1 1 822.71319 2466.12502 2466.12285 0.88 0.00072 913977.5 CID (Collision Induced Dissociation) MS2 0.0 60 13.0 60.0 64.964 19547 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 11.5 10.1 11.6 9.3 16.5 16.7 16.2 13.2 12.9 12.3 Excluded by Method 2.37 1 0.002055 0.006939 False High Sequest HT (A2) Unambiguous EPLIPASPK [K].ePLIPAsPk.[A] N-Term(TMT6plex); S7(Phospho); K9(TMT6plex) 1 Q9Y613 Q9Y613 0 3 0.4623 0 1 1 497.28582 1489.84291 1489.84315 -0.16 -8e-05 784655.25 CID (Collision Induced Dissociation) MS2 0.0 30 72.7 60.0 86.22 27589 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 69.0 76.6 53.4 87.5 80.6 69.5 78.9 73.8 69.2 68.5 Excluded by Method 1.99 1 0.002055 0.007788 False High Sequest HT (A2) Unambiguous NWGAVVRSPEGTPQK [K].nWGAVVRsPEGTPQk.[I] N-Term(TMT6plex); S8(Phospho); K15(TMT6plex) 2 Q86WB0 Q86WB0-3; Q86WB0 1 3 0.1982 0 1 1 722.04886 2164.13203 2164.13162 0.19 0.00013 3320932.0 CID (Collision Induced Dissociation) MS2 0.0 20 64.3 38.77 78.7215 24766 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 66.0 57.4 49.4 70.2 64.4 70.6 68.8 68.3 69.6 58.7 Excluded by Method 2.17 1 0.002055 0.007712 False High Sequest HT (A2) Unambiguous NTGVSPASRPSPGTPTSPSNLTSGLK [K].nTGVSPASRPSPGTPTsPSNLTSGLk.[T] N-Term(TMT6plex); S17(Phospho); K26(TMT6plex) 4 Q5VT52 Q5VT52-5; Q5VT52; Q5VT52-2; Q5VT52-3 0 4 0.012 0 1 1 762.89995 3048.57798 3048.57671 0.42 0.00032 1166261.25 CID (Collision Induced Dissociation) MS2 11.47156 60 62.7 60.0 83.8676 26711 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 60.8 57.2 54.0 74.4 56.2 70.9 77.5 59.9 66.4 49.9 Excluded by Method 2.49 1 0.002055 0.007166 False High Sequest HT (A2) Unambiguous SLQPFDDLLGDFNK [K].sLQPFDDLLGDFNk.[V] N-Term(TMT6plex); S1(Phospho); K14(TMT6plex) 1 Q06336 Q06336 0 3 0.2634 0 1 1 716.36534 2147.08147 2147.08261 -0.53 -0.00038 1890245.375 CID (Collision Induced Dissociation) MS2 0.0 40 102.0 43.97 130.5907 44313 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 42.6 89.2 138.0 40.0 80.6 178.4 49.0 102.8 192.0 107.5 Excluded by Method 2.05 1 0.002055 0.008273 False High Sequest HT (A2) Unambiguous GPQPPTVSPIRSSRPPPAK [K].gPQPPTVsPIRSSRPPPAk.[R] N-Term(TMT6plex); S8(Phospho); K19(TMT6plex) 3 P46087 P46087-2; P46087; P46087-4 1 4 0.0892 0 1 1 627.60243 2507.38789 2507.38996 -0.83 -0.00052 1168106.5 CID (Collision Induced Dissociation) MS2 44.2232 30 59.2 60.0 63.2809 18920 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 59.1 49.1 54.8 57.1 53.9 60.2 69.3 66.8 59.1 62.6 Excluded by Method 4.15 1 0.002055 0.008039 False High Sequest HT (A2) Unambiguous LDTVSDYAASVTTPVK [K].lDTVSDYAASVTtPVk.[E] N-Term(TMT6plex); T13(Phospho); K16(TMT6plex) 1 P53148 P53148 0 3 0.0062 0 1 1 735.72017 2205.14596 2205.1456 0.16 0.00012 525003.875 CID (Collision Induced Dissociation) MS2 40.39002 60 28.0 60.0 106.9005 35333 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 19.4 29.4 39.7 12.3 26.7 41.4 15.5 25.0 42.1 28.8 Excluded by Method 1.62 1 0.002055 0.007466 False High Sequest HT (A2) Unambiguous VLDEEGSEREFDEDSDEK [K].vLDEEGsEREFDEDsDEk.[E] N-Term(TMT6plex); S7(Phospho); S15(Phospho); K18(TMT6plex) 1 O43719 O43719 1 3 0.4377 0 1 1 916.0522 2746.14203 2746.14218 -0.05 -5e-05 807276.125 CID (Collision Induced Dissociation) MS2 0.0 20 75.4 60.0 87.0182 27885 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 73.5 69.4 69.4 81.6 74.0 78.7 81.1 79.7 71.6 74.9 Excluded by Method 3.45 1 0.002055 0.007488 False High Sequest HT (A2) Unambiguous ALVLELDEMSNGSEIQDALEEISGQK [K].aLVLELDEmSNGsEIQDALEEISGQk.[T] N-Term(TMT6plex); M9(Oxidation); S13(Phospho); K26(TMT6plex) 2 P17695 P17695; P17695-2 0 3 0.4387 0 1 1 1124.8975 3372.67794 3372.65337 7.28 0.00819 247606.75 CID (Collision Induced Dissociation) MS2 12.73877 70 5.8 60.0 146.129 49924 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 1.9 5.8 8.1 1.7 2.1 11.0 2.3 6.9 12.4 5.9 2.53 1 0.002055 0.007208 False High Sequest HT (A2) Unambiguous RIACEEEFSDSEEEGEGGRK [K].rIAcEEEFsDsEEEGEGGRk.[N] N-Term(TMT6plex); C4(Carbamidomethyl); S9(Phospho); S11(Phospho); K20(TMT6plex) 1 Q13547 Q13547 2 3 0.4876 0 1 1 978.08741 2932.24767 2932.24733 0.12 0.00011 1143273.25 CID (Collision Induced Dissociation) MS2 0.0 30 122.0 60.0 63.3059 18970 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 126.9 121.6 94.3 128.0 120.8 121.2 143.9 121.3 121.1 121.1 Excluded by Method 2.42 1 0.002055 0.007241 False High Sequest HT (A2) Unambiguous IGEGTYGVVYK [K].iGEGTYGVVyk.[AG] N-Term(TMT6plex); Y10(Phospho); K11(TMT6plex) 5 P24941 P24941; P06493; Q00526; P24941-2; P06493-2 0 3 0.2242 0 1 1 575.30743 1723.90773 1723.90721 0.3 0.00017 610252.6875 CID (Collision Induced Dissociation) MS2 5.386886 40 47.5 60.0 91.8322 29613 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 40.2 46.3 33.2 43.7 48.8 43.7 61.6 51.3 54.8 51.8 Excluded by Method 3.3 1 0.002055 0.007803 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVssDEEF.[-] N-Term(TMT6plex); S8(Phospho); S9(Phospho) 1 P16521 P16521 0 2 0.1818 0 1 1 925.35435 1849.70142 1849.70195 -0.29 -0.00027 493719.875 CID (Collision Induced Dissociation) MS2 10.88682 50 46.8 60.0 142.4581 48608 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 11.8 38.3 73.4 15.7 35.0 91.5 18.3 48.4 92.6 43.1 Excluded by Method 2.75 1 0.002055 0.007047 False High Sequest HT (A2) Unambiguous RSPSPEEK [K].rSPsPEEk.[A] N-Term(TMT6plex); S4(Phospho); K8(TMT6plex) 1 P25686 P25686 1 3 0.1522 0 1 1 489.92506 1467.76064 1467.76088 -0.16 -8e-05 321668.0 CID (Collision Induced Dissociation) MS2 6.14626 20 20.6 60.0 32.7009 7757 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 21.5 21.9 13.7 24.0 20.4 24.9 21.9 20.9 19.7 17.6 Excluded by Method 1.84 1 0.002055 0.007248 False High Sequest HT (A2) Unambiguous APSLTDLVK [K].aPsLTDLVk.[S] N-Term(TMT6plex); S3(Phospho); K9(TMT6plex) 1 O15014 O15014 0 2 0.1642 0 1 1 741.42366 1481.84004 1481.83807 1.33 0.00098 1678566.0 CID (Collision Induced Dissociation) MS2 18.42302 10 245.8 60.0 110.4558 36655 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 96.4 209.5 385.3 115.8 204.1 418.9 125.2 238.5 431.2 233.1 Excluded by Method 2.68 1 0.002055 0.006927 False High Sequest HT (A2) Unambiguous ITIADCGQLE [K].iTIADcGQLE.[-] N-Term(TMT6plex); C6(Carbamidomethyl) 2 P62937 P62937; P62937-2 0 2 0.6356 0 1 1 674.8529 1348.69853 1348.69798 0.4 0.00027 8292494.5 CID (Collision Induced Dissociation) MS2 0.0 20 107.4 34.817 94.1887 30490 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 89.8 99.6 89.3 99.5 105.1 119.8 121.7 117.0 107.4 124.4 Excluded by Method 2.25 1 0.002055 0.008562 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVsSDEEF.[-] N-Term(TMT6plex); S8(Phospho) 1 P16521 P16521 0 2 0.0468 0 1 1 885.37528 1769.74328 1769.73562 4.33 0.00383 688632.6875 CID (Collision Induced Dissociation) MS2 61.34328 40 50.8 60.0 133.003 45312 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 20.1 40.7 75.3 17.3 33.4 106.0 18.0 53.6 95.9 47.5 Excluded by Method 2.99 1 0.002055 0.008454 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVssDEEF.[-] N-Term(TMT6plex); S8(Phospho); S9(Phospho) 1 P16521 P16521 0 2 0.2121 0 1 1 925.3549 1849.70252 1849.70195 0.31 0.00028 865816.625 CID (Collision Induced Dissociation) MS2 42.31402 40 64.4 60.0 133.331 45433 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 21.2 52.1 96.3 21.2 50.6 123.1 28.9 58.6 127.1 65.1 Excluded by Method 2.31 1 0.002055 0.008395 False High Sequest HT (A2) Unambiguous RFVSEGDGGRLKPESY [K].rFVsEGDGGRLkPESY.[-] N-Term(TMT6plex); S4(Phospho); K12(TMT6plex) 2 Q53EL6 Q53EL6-2; Q53EL6 2 4 0.2973 0 1 1 584.55189 2335.18573 2335.18478 0.41 0.00024 1243477.125 CID (Collision Induced Dissociation) MS2 4.418646 20 65.6 60.0 72.6825 22439 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 60.6 62.3 54.8 72.2 61.4 69.3 66.7 74.2 68.3 66.0 Excluded by Method 2.96 1 0.002055 0.007386 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVSsDEEF.[-] N-Term(TMT6plex); S9(Phospho) 1 P16521 P16521 0 2 0.0029 0 1 1 885.36911 1769.73095 1769.73562 -2.64 -0.00234 379404.0625 CID (Collision Induced Dissociation) MS2 22.23535 40 47.5 60.0 136.4943 46548 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 11.1 38.1 71.2 14.7 32.5 87.8 22.0 47.8 96.6 53.1 Excluded by Method 3.43 1 0.002055 0.008388 False High Sequest HT (A2) Unambiguous RTCGVNDDESPSK [K].rTcGVNDDEsPSk.[I] N-Term(TMT6plex); C3(Carbamidomethyl); S10(Phospho); K13(TMT6plex) 1 Q8NG08 Q8NG08 1 3 0.1702 0 1 1 668.31539 2002.93161 2002.93054 0.53 0.00036 154566.484375 CID (Collision Induced Dissociation) MS2 33.04713 50 23.8 60.0 37.9272 9624 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 24.3 27.3 20.7 28.7 23.1 21.6 26.4 22.7 22.8 20.0 Excluded by Method 2.35 1 0.002055 0.006992 False High Sequest HT (A2) Unambiguous SHHANSPTAGAAK [K].sHHANsPTAGAAk.[S] N-Term(TMT6plex); S6(Phospho); K13(TMT6plex) 5 Q15555 Q15555-2; Q15555-3; Q15555-5; Q15555-4; Q15555 0 3 0.1958 0 1 1 596.30493 1786.90023 1786.90017 0.03 2e-05 63825.46875 CID (Collision Induced Dissociation) MS2 0.0 60 2.6 60.0 22.6924 4483 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 1.3 2.8 1.6 3.6 2.7 3.3 2.2 2.0 2.4 3.8 Excluded by Method 1.89 1 0.002055 0.008409 False High Sequest HT (A2) Unambiguous SASPVLPGVK [K].sASPVLPGVk.[R] N-Term(TMT6plex); S1(Phospho); K10(TMT6plex) 1 P38272 P38272 0 3 0.3616 0 1 1 498.2898 1492.85484 1492.85405 0.53 0.00026 686306.75 CID (Collision Induced Dissociation) MS2 10.27628 40 58.1 60.0 84.7053 26957 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 15.3 42.5 96.1 16.9 51.7 111.1 22.6 48.5 110.5 66.1 Excluded by Method 2.71 1 0.002055 0.008522 False High Sequest HT (A2) Unambiguous RTCGVNDDESPSK [K].rTcGVNDDEsPSk.[I] N-Term(TMT6plex); C3(Carbamidomethyl); S10(Phospho); K13(TMT6plex) 1 Q8NG08 Q8NG08 1 3 0.1572 0 1 1 668.31552 2002.932 2002.93054 0.73 0.00049 292040.53125 CID (Collision Induced Dissociation) MS2 4.269719 30 55.9 60.0 38.0623 9668 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 46.4 62.1 45.0 64.0 53.0 52.7 71.4 53.7 60.1 50.8 Excluded by Method 2.99 1 0.002055 0.007287 False High Sequest HT (A2) Unambiguous VSESTSIGK [K].vSESTsIGk.[G] N-Term(TMT6plex); S6(Phospho); K9(TMT6plex) 1 P53278 P53278 0 2 0.0609 0 1 1 723.3874 1445.76752 1445.7653 1.54 0.00111 1399896.625 CID (Collision Induced Dissociation) MS2 39.43712 30 170.0 60.0 61.4218 18226 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 40.7 129.0 251.9 56.7 140.3 336.4 61.0 166.0 337.8 180.7 Excluded by Method 3.12 1 0.002055 0.007643 False High Sequest HT (A2) Unambiguous ENRQSIINPDWNFEK [K].eNRQsIINPDWNFEk.[M] N-Term(TMT6plex); S5(Phospho); K15(TMT6plex) 2 P46459 P46459; P46459-2 1 3 0.4027 0 1 1 810.07403 2428.20754 2428.20625 0.53 0.00043 608113.125 CID (Collision Induced Dissociation) MS2 8.092286 50 22.0 60.0 99.9949 32680 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 24.2 20.9 19.9 20.1 27.6 27.7 21.0 16.5 30.0 12.5 Excluded by Method 3.7 1 0.002055 0.006933 False High Sequest HT (A2) Unambiguous DSNELSDSAGEEDSADLK [K].dSNELsDsAGEEDSADLk.[R] N-Term(TMT6plex); S6(Phospho); S8(Phospho); K18(TMT6plex) 2 Q9Y6X9 Q9Y6X9-2; Q9Y6X9 0 3 0.1288 0 1 1 834.01975 2500.0447 2500.04174 1.18 0.00099 311287.875 CID (Collision Induced Dissociation) MS2 68.43494 30 27.0 60.0 76.8243 24054 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 22.7 27.1 20.6 26.0 24.9 27.9 35.6 28.8 29.5 27.2 Excluded by Method 2.64 1 0.002055 0.007836 False High Sequest HT (A2) Unambiguous SLHPWYGITPTSSPK [K].sLHPWYGItPtSSPk.[T] N-Term(TMT6plex); T9(Phospho); T11(Phospho); K15(TMT6plex) 1 Q8N3F8 Q8N3F8 0 3 0.0027 0 1 1 763.70934 2289.11346 2289.11221 0.55 0.00042 860669.0 CID (Collision Induced Dissociation) MS2 11.76796 40 52.8 60.0 106.756 35281 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 43.8 55.8 45.0 48.5 48.2 47.0 65.8 55.3 60.3 58.6 Excluded by Method 3.66 1 0.002055 0.007497 False High Sequest HT (A2) Unambiguous KEESEESDDDMGFGLFD [K].kEESEEsDDDmGFGLFD.[-] N-Term(TMT6plex); K1(TMT6plex); S7(Phospho); M11(Oxidation) 3 P05387 P05386; P05386-2; P05387 1 3 0.0638 0 1 1 835.3541 2504.04775 2504.04642 0.53 0.00044 3438538.25 CID (Collision Induced Dissociation) MS2 6.086692 20 174.1 20.527 114.215 38056 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 170.7 162.3 134.6 191.2 171.1 170.1 195.6 185.0 183.5 177.2 Excluded by Method 1.88 1 0.002055 0.007643 False High Sequest HT (A2) Unambiguous LSGFSFK [K].lSGFsFk.[K] N-Term(TMT6plex); S5(Phospho); K7(TMT6plex) 1 P29966 P29966 0 2 0.3719 0 1 1 662.3594 1323.71151 1323.71141 0.08 5e-05 8706601.0 CID (Collision Induced Dissociation) MS2 2.989156 10 178.5 30.735 112.5583 37372 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 184.0 180.7 154.0 207.2 166.2 155.4 222.3 185.1 173.2 156.7 Excluded by Method 2.85 1 0.002079 0.009329 False High Sequest HT (A2) Unambiguous NTLETSSLNFK [K].nTLETsSLNFk.[V] N-Term(TMT6plex); S6(Phospho); K11(TMT6plex) 1 Q14151 Q14151 0 3 0.0065 0 1 1 597.98097 1791.92836 1791.9294 -0.58 -0.00035 1954857.875 CID (Collision Induced Dissociation) MS2 23.95581 50 48.8 51.299 99.2859 32417 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 29.0 42.1 47.8 35.5 44.6 59.3 46.6 55.6 73.7 54.0 Excluded by Method 3.1 1 0.002079 0.008715 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVsSDEEF.[-] N-Term(TMT6plex); S8(Phospho) 1 P16521 P16521 0 2 0.01 0 1 1 885.37291 1769.73854 1769.73562 1.65 0.00146 1095831.25 CID (Collision Induced Dissociation) MS2 47.40496 70 41.1 55.805 132.3584 45090 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 15.4 38.9 67.4 13.9 31.8 81.2 16.8 34.0 72.1 40.0 3.0 1 0.002079 0.01056 False High Sequest HT (A2) Unambiguous DTSTSADAEASEDQNK [K].dtSTSADAEASEDQNk.[E] N-Term(TMT6plex); T2(Phospho); K16(TMT6plex) 1 P53885 P53885 0 3 0.0791 0 1 1 736.3308 2206.97785 2206.9753 1.15 0.00085 337224.53125 CID (Collision Induced Dissociation) MS2 0.0 80 6.1 60.0 45.5171 12360 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 2.3 7.0 6.8 3.9 2.1 10.6 7.7 6.5 10.5 3.5 1.39 1 0.002079 0.009731 False High Sequest HT (A2) Unambiguous TASESISNLSEAGSIK [K].tASESISNLSEAGsIk.[K] N-Term(TMT6plex); S14(Phospho); K16(TMT6plex) 3 P30622 P30622-2; P30622; P30622-1 0 3 0.1722 0 1 1 711.36755 2132.08809 2132.08882 -0.34 -0.00024 1626880.75 CID (Collision Induced Dissociation) MS2 0.0 40 28.1 60.0 88.8893 28505 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 25.2 24.4 26.2 29.3 27.8 31.9 27.3 29.9 30.0 29.0 Excluded by Method 2.73 1 0.002079 0.008673 False High Sequest HT (A2) Unambiguous DDSHSAEDSEDEK [K].dDSHsAEDsEDEk.[E] N-Term(TMT6plex); S5(Phospho); S9(Phospho); K13(TMT6plex) 2 Q9H1E3 Q9H1E3; Q9H1E3-2 0 3 0.4273 0 1 1 694.60448 2081.79888 2081.79899 -0.05 -4e-05 253921.6875 CID (Collision Induced Dissociation) MS2 0.0 30 25.2 60.0 35.0184 8582 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 21.2 30.5 14.9 27.6 24.7 26.6 25.3 27.1 26.7 27.5 Excluded by Method 2.27 1 0.002079 0.009062 False High Sequest HT (A2) Unambiguous ALVLELDEMSNGSEIQDALEEISGQK [K].aLVLELDEmsNGSEIQDALEEISGQk.[T] N-Term(TMT6plex); M9(Oxidation); S10(Phospho); K26(TMT6plex) 2 P17695 P17695; P17695-2 0 3 0.0048 0 1 1 1124.89444 3372.66878 3372.65337 4.57 0.00514 172408.0625 CID (Collision Induced Dissociation) MS2 39.27225 50 7.6 60.0 143.5227 49018 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 2.2 6.5 12.0 1.3 3.0 15.4 7.5 9.0 12.8 6.3 Excluded by Method 2.09 1 0.002079 0.01115 False High Sequest HT (A2) Unambiguous ASAPERTPSPAPK [K].aSAPERtPsPAPk.[R] N-Term(TMT6plex); T7(Phospho); S9(Phospho); K13(TMT6plex) 4 Q63ZY6 Q63ZY6-6; Q63ZY6; Q96P11-2; Q3KNT7-3 1 3 0.3038 0 1 1 642.98797 1926.94935 1926.94917 0.1 6e-05 212767.53125 CID (Collision Induced Dissociation) MS2 0.0 30 290.5 60.0 45.7527 12444 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 285.8 297.7 248.1 280.9 273.5 311.5 312.4 309.3 289.5 296.8 Excluded by Method 3.16 1 0.002079 0.008744 False High Sequest HT (A2) Unambiguous ELEAERSLMSSPEDLTK [K].eLEAERSLmssPEDLTk.[D] N-Term(TMT6plex); M9(Oxidation); S10(Phospho); S11(Phospho); K17(TMT6plex) 1 P46821 P46821 1 3 0.3136 0 1 1 857.06913 2569.19282 2569.19099 0.71 0.00061 1548804.125 CID (Collision Induced Dissociation) MS2 38.30664 30 157.8 55.706 90.8668 29246 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 150.8 157.6 122.2 172.0 154.8 152.8 181.0 171.9 164.3 150.9 Excluded by Method 3.54 1 0.002079 0.009657 False High Sequest HT (A2) Unambiguous GSQESTNTLEEIVK [K].gSQEStNtLEEIVk.[F] N-Term(TMT6plex); T6(Phospho); T8(Phospho); K14(TMT6plex) 1 P35845 P35845 0 3 0.0932 0 1 1 718.34568 2153.0225 2153.01803 2.07 0.00149 1330777.375 CID (Collision Induced Dissociation) MS2 0.0 40 117.6 60.0 103.9108 34142 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 40.3 106.5 181.4 37.4 100.7 212.7 43.4 108.7 223.6 121.3 Excluded by Method 4.29 1 0.002079 0.008635 False High Sequest HT (A2) Unambiguous SSEASPTAQRSK [K].sSEAsPTAQRSk.[D] N-Term(TMT6plex); S5(Phospho); K12(TMT6plex) 1 Q7Z569 Q7Z569 1 3 0.187 0 1 1 596.30859 1786.91121 1786.91006 0.64 0.00038 381628.0 CID (Collision Induced Dissociation) MS2 5.668203 20 44.8 60.0 30.7604 7077 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 49.4 40.0 41.6 44.3 45.8 45.9 48.9 49.0 41.9 41.6 Excluded by Method 2.46 1 0.002079 0.01216 False High Sequest HT (A2) Unambiguous CSGPGLSPGMVRANLPQSFQVDTSK [K].cSGPGLsPGmVRANLPQSFQVDTSk.[A] N-Term(TMT6plex); C1(Carbamidomethyl); S7(Phospho); M10(Oxidation); K25(TMT6plex) 2 P21333 P21333; P21333-2 1 3 0.1451 0 1 1 1063.19265 3187.5634 3187.56814 -1.49 -0.00158 430753.5 CID (Collision Induced Dissociation) MS2 0.0 40 70.5 60.0 108.825 35978 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 66.4 69.8 63.7 79.9 58.5 72.9 74.6 69.8 84.5 65.1 Excluded by Method 3.24 1 0.002079 0.01145 False High Sequest HT (A2) Unambiguous AAADALSDLEIK [K].aAADALsDLEIk.[D] N-Term(TMT6plex); S7(Phospho); K12(TMT6plex) 1 P09938 P09938 0 3 0.0449 0 1 1 585.64945 1754.9338 1754.93415 -0.2 -0.00012 405530.46875 CID (Collision Induced Dissociation) MS2 49.92802 40 49.7 60.0 114.7703 38188 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 13.3 42.9 68.6 15.8 45.6 90.1 23.4 49.9 91.3 56.5 Excluded by Method 2.67 1 0.002079 0.01264 False High Sequest HT (A2) Unambiguous LFEESDDK [K].lFEEsDDk.[E] N-Term(TMT6plex); S5(Phospho); K8(TMT6plex) 1 O43719 O43719 0 3 0.4342 0 1 1 507.58075 1520.72768 1520.72858 -0.59 -0.0003 1201689.25 CID (Collision Induced Dissociation) MS2 0.0 20 23.6 60.0 88.0363 28187 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 16.3 20.1 22.5 18.5 22.9 33.4 24.2 21.6 30.0 26.9 Excluded by Method 2.81 1 0.002079 0.01125 False High Sequest HT (A2) Unambiguous ALEEVSLK [K].aLEEVsLk.[W] N-Term(TMT6plex); S6(Phospho); K8(TMT6plex) 1 P14126 P14126 0 2 0.2836 0 1 1 713.90184 1426.79641 1426.79587 0.38 0.00027 849964.625 CID (Collision Induced Dissociation) MS2 7.980466 20 49.3 60.0 91.0439 29315 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 23.4 47.5 64.2 24.3 43.8 73.3 24.9 52.3 87.2 52.0 Excluded by Method 3.35 1 0.002079 0.01021 False High Sequest HT (A2) Unambiguous EEEAKEESDDDMGFGLFD [K].eEEAkEEsDDDMGFGLFD.[-] N-Term(TMT6plex); K5(TMT6plex); S8(Phospho) 1 P02400 P02400 1 3 0.2353 0 1 1 867.70422 2601.09812 2601.09918 -0.41 -0.00035 2471826.0 CID (Collision Induced Dissociation) MS2 39.73275 60 60.8 32.739 128.5072 43445 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 41.9 56.5 61.5 38.4 50.7 93.3 45.8 65.9 94.9 59.0 Excluded by Method 2.38 1 0.002079 0.01131 False High Sequest HT (A2) Unambiguous NAEEESESEAEEGD [K].nAEEEsEsEAEEGD.[-] N-Term(TMT6plex); S6(Phospho); S8(Phospho) 4 Q7L1Q6 Q7L1Q6-2; Q7L1Q6-4; Q7L1Q6-3; Q7L1Q6 0 2 0.9103 0 1 1 957.32483 1913.64238 1913.6412 0.61 0.00059 179199.359375 CID (Collision Induced Dissociation) MS2 0.0 60 7.3 60.0 41.3851 10872 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 4.0 7.7 3.0 3.5 12.1 7.1 10.6 8.8 9.6 6.9 Excluded by Method 2.23 1 0.002079 0.009653 False High Sequest HT (A2) Unambiguous ASLRRVELSGPK [K].asLRRVELSGPk.[A] N-Term(TMT6plex); S2(Phospho); K12(TMT6plex) 4 Q9UHD8 Q9UHD8-7; Q9UHD8-2; Q9UHD8-5; Q9UHD8 2 4 0.0952 0 1 1 463.5206 1851.06057 1851.06175 -0.64 -0.0003 305576.71875 CID (Collision Induced Dissociation) MS2 0.0 30 30.4 60.0 67.7585 20635 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 29.7 34.1 26.4 33.7 29.3 31.2 34.9 32.3 28.7 23.5 Excluded by Method 1.68 1 0.002079 0.01176 False High Sequest HT (A2) Unambiguous SSVALQDLIK [K].sSVALQDLIk.[H] N-Term(TMT6plex); S1(Phospho); K10(TMT6plex) 1 P47116 P47116 0 3 0.0723 0 1 1 537.97557 1611.91215 1611.9123 -0.09 -5e-05 275173.8125 CID (Collision Induced Dissociation) MS2 27.56782 50 11.9 60.0 117.9502 39441 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 2.2 12.9 21.9 2.6 9.4 22.1 5.9 11.8 18.4 11.4 Excluded by Method 2.49 1 0.002079 0.01016 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVSsDEEF.[-] N-Term(TMT6plex); S9(Phospho) 1 P16521 P16521 0 2 0.0426 0 1 1 885.37175 1769.73622 1769.73562 0.34 0.0003 808258.25 CID (Collision Induced Dissociation) MS2 11.72031 40 65.4 60.0 122.0779 40965 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 19.1 58.7 98.6 22.2 54.7 106.0 27.2 61.7 133.1 72.5 Excluded by Method 2.58 1 0.002079 0.01175 False High Sequest HT (A2) Unambiguous DDDEEPSDSEDK [K].dDDEEPsDSEDk.[E] N-Term(TMT6plex); S7(Phospho); K12(TMT6plex) 1 P09232 P09232 0 2 0.1617 0 1 1 959.89727 1918.78727 1918.78431 1.54 0.00148 102142.28125 CID (Collision Induced Dissociation) MS2 0.0 50 13.3 60.0 43.2161 11526 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 3.2 12.3 15.8 3.5 7.3 28.0 2.9 12.3 32.0 16.1 Excluded by Method 2.66 1 0.002079 0.009151 False High Sequest HT (A2) Unambiguous EEEEAKEESDDDMGFGLFD [K].eEEEAkEEsDDDmGFGLFD.[-] N-Term(TMT6plex); K6(TMT6plex); S9(Phospho); M13(Oxidation) 1 P05318 P05318 1 3 0.4792 0 1 1 916.0503 2746.13636 2746.13669 -0.12 -0.00011 553244.4375 CID (Collision Induced Dissociation) MS2 25.22136 50 92.6 60.0 120.0372 40217 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 19.5 75.0 137.3 29.0 73.3 169.8 27.9 90.5 211.9 91.9 Excluded by Method 2.88 1 0.002079 0.008753 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVSsDEEF.[-] N-Term(TMT6plex); S9(Phospho) 1 P16521 P16521 0 2 0.0643 0 1 1 885.37187 1769.73646 1769.73562 0.47 0.00042 223450.5 CID (Collision Induced Dissociation) MS2 0.0 50 28.2 60.0 139.5141 47595 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 6.3 27.9 45.8 9.3 15.4 48.5 13.8 25.7 58.7 30.7 Excluded by Method 2.49 1 0.002079 0.009511 False High Sequest HT (A2) Unambiguous IESPESIK [K].iEsPEsIk.[E] N-Term(TMT6plex); S3(Phospho); S6(Phospho); K8(TMT6plex) 1 P23201 P23201 0 2 0.4047 0 1 1 760.87484 1520.74241 1520.74146 0.62 0.00047 569720.5 CID (Collision Induced Dissociation) MS2 30.54533 30 119.7 60.0 84.8372 26986 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 35.8 93.1 172.5 48.3 102.4 216.7 47.2 108.1 251.9 120.9 Excluded by Method 2.99 1 0.002079 0.009998 False High Sequest HT (A2) Unambiguous EAPSPTLRRK [K].eAPsPTLRRk.[L] N-Term(TMT6plex); S4(Phospho); K10(TMT6plex) 3 P25054 P25054-3; P25054; P25054-2 2 4 0.1678 0 1 1 423.99449 1692.95612 1692.95623 -0.06 -3e-05 156496.578125 CID (Collision Induced Dissociation) MS2 10.14003 30 38.4 60.0 43.6414 11682 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 45.3 36.6 29.7 37.0 33.4 42.0 39.6 37.4 36.4 46.6 Excluded by Method 2.92 1 0.002079 0.008995 False High Sequest HT (A2) Unambiguous VGSTENIK [K].vGsTENIk.[H] N-Term(TMT6plex); S3(Phospho); K8(TMT6plex) 4 P27816 P27816; P27816-5; P27816-6; P27816-2 0 2 0.0906 0 1 1 693.37598 1385.74468 1385.74417 0.37 0.00026 711937.1875 CID (Collision Induced Dissociation) MS2 28.22304 10 76.7 60.0 62.6777 18731 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 73.8 73.1 58.3 88.8 72.0 76.0 81.9 81.3 86.1 75.3 Excluded by Method 3.2 1 0.002079 0.0106 False High Sequest HT (A2) Unambiguous VEVTSEDEK [K].vEVTsEDEk.[E] N-Term(TMT6plex); S5(Phospho); K9(TMT6plex) 1 P07264 P07264 0 2 0.1281 0 1 1 787.39102 1573.77475 1573.77626 -0.95 -0.00075 747853.1875 CID (Collision Induced Dissociation) MS2 9.91293 30 75.8 60.0 62.68 18732 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 26.0 58.2 113.2 24.6 63.5 140.2 29.6 68.2 151.0 83.6 Excluded by Method 2.42 1 0.002079 0.01082 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVSsDEEF.[-] N-Term(TMT6plex); S9(Phospho) 1 P16521 P16521 0 2 0.0036 0 1 1 885.37193 1769.73658 1769.73562 0.54 0.00048 276150.4375 CID (Collision Induced Dissociation) MS2 0.0 50 34.1 60.0 146.4677 49984 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 7.5 31.4 51.8 13.7 27.2 61.9 12.6 35.6 69.2 30.4 Excluded by Method 2.81 1 0.002079 0.01256 False High Sequest HT (A2) Unambiguous QTGERSIFSQEVVELPDSEDETQQVNK [K].qTGERSIFSQEVVELPDsEDETQQVNk.[T] N-Term(TMT6plex); S18(Phospho); K27(TMT6plex) 1 P53885 P53885 1 4 0.0701 0 1 1 908.44574 3630.76113 3630.75765 0.96 0.00087 804535.3125 CID (Collision Induced Dissociation) MS2 0.0 50 19.7 60.0 105.1764 34613 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 5.4 13.7 31.5 4.5 21.1 38.7 7.4 20.0 35.8 18.5 Excluded by Method 3.28 1 0.002079 0.008821 False High Sequest HT (A2) Unambiguous GEAAAERPGEAAVASSPSK [K].gEAAAERPGEAAVASSPsk.[A] N-Term(TMT6plex); S18(Phospho); K19(TMT6plex) 1 P29966 P29966 0 3 0.1373 0 1 1 775.06107 2323.16866 2323.16953 -0.37 -0.00029 457688.875 CID (Collision Induced Dissociation) MS2 3.900376 20 16.5 60.0 52.5687 14961 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 15.4 15.1 12.5 14.7 15.6 17.0 17.4 18.8 18.1 20.1 Excluded by Method 2.84 1 0.002079 0.01133 False High Sequest HT (A2) Unambiguous ETTYDEIK [K].eTTYDEIk.[K] N-Term(TMT6plex); K8(TMT6plex) 2 P00359 P00359; P00358 0 2 0.2892 0 1 1 728.90078 1456.79429 1456.79355 0.51 0.00037 1810187.75 CID (Collision Induced Dissociation) MS2 18.46683 20 159.8 58.643 63.2883 18963 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 71.3 130.6 200.9 95.4 144.7 272.6 89.2 161.4 272.5 159.7 Excluded by Method 2.49 1 0.002079 0.009264 False High Sequest HT (A2) Unambiguous YDSPVSSPITSASELGSIAK [K].yDsPVsSPITSASELGSIAk.[L] N-Term(TMT6plex); S3(Phospho); S6(Phospho); K20(TMT6plex) 1 P53890 P53890 0 3 0.1794 0 1 1 876.42699 2627.26643 2627.26587 0.21 0.00019 520313.0625 CID (Collision Induced Dissociation) MS2 21.09152 50 111.6 60.0 126.4406 42559 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 37.3 90.8 164.4 41.0 90.8 217.6 42.2 102.5 222.0 107.5 Excluded by Method 2.23 1 0.002079 0.008707 False High Sequest HT (A2) Unambiguous YAAAAPAATSAASGDAAPAEEAAAEEEEESDDDMGFGLFD [K].yAAAAPAATSAASGDAAPAEEAAAEEEEEsDDDMGFGLFD.[-] N-Term(TMT6plex); S30(Phospho) 1 P05317 P05317 0 4 0.1196 0 1 1 1058.18944 4229.73591 4229.73795 -0.48 -0.00051 165534.359375 CID (Collision Induced Dissociation) MS2 23.08935 40 5.3 60.0 144.8267 49422 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 2.4 4.2 7.9 3.8 16.6 3.9 11.3 3.2 Excluded by Method 2.09 1 0.002079 0.01279 False High Sequest HT (A2) Unambiguous ESLSPGK [K].eSLsPGk.[L] N-Term(TMT6plex); S4(Phospho); K7(TMT6plex) 3 Q0ZGT2 Q0ZGT2-2; Q0ZGT2-4; Q0ZGT2 0 2 0.2677 0 1 1 628.33875 1255.67023 1255.66994 0.23 0.00015 4610920.0 CID (Collision Induced Dissociation) MS2 5.651575 30 111.0 47.888 54.8868 15820 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 116.3 107.4 77.5 112.8 115.2 106.7 128.0 118.3 118.0 109.6 Excluded by Method 2.54 1 0.002079 0.009881 False High Sequest HT (A2) Unambiguous ALVLELDEMSNGSEIQDALEEISGQK [K].aLVLELDEmsNGSEIQDALEEISGQk.[T] N-Term(TMT6plex); M9(Oxidation); S10(Phospho); K26(TMT6plex) 2 P17695 P17695; P17695-2 0 3 0.0102 0 1 1 1124.88993 3372.65523 3372.65337 0.55 0.00062 238164.421875 CID (Collision Induced Dissociation) MS2 38.15899 50 8.1 60.0 144.5205 49319 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 1.9 6.4 12.9 2.0 7.0 17.5 3.0 7.0 17.0 6.7 Excluded by Method 1.97 1 0.002079 0.009958 False High Sequest HT (A2) Unambiguous GSSERGSPIEK [K].gSSERGsPIEk.[Y] N-Term(TMT6plex); S7(Phospho); K11(TMT6plex) 3 Q9UHF7 Q9UHF7-3; Q9UHF7-2; Q9UHF7 1 3 0.3022 0 1 1 562.29435 1684.8685 1684.86714 0.81 0.00046 130648.9296875 CID (Collision Induced Dissociation) MS2 60.26915 20 155.4 60.0 40.5063 10555 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 151.4 159.2 123.5 154.0 153.6 148.5 170.9 165.2 159.8 168.3 Excluded by Method 2.68 1 0.002079 0.0107 False High Sequest HT (A2) Unambiguous MEGSDFESSGGRGLK [K].mEGsDFESSGGRGLk.[K] N-Term(TMT6plex); M1(Oxidation); S4(Phospho); K15(TMT6plex) 2 Q8WYH8 Q8WYH8-2; Q8WYH8 1 3 0.3368 0 1 1 704.33422 2110.9881 2110.98806 0.02 2e-05 1553048.375 CID (Collision Induced Dissociation) MS2 0.0 10 134.3 60.0 61.6362 18340 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 137.7 131.6 119.8 129.9 139.5 125.1 144.4 141.0 139.5 134.5 Excluded by Method 1.93 1 0.002079 0.009113 False High Sequest HT (A2) Unambiguous AEDGATPSPSNETPK [K].aEDGAtPSPsNETPk.[K] N-Term(TMT6plex); T6(Phospho); S10(Phospho); K15(TMT6plex) 1 P29966 P29966 0 3 0.124 0 1 1 706.9857 2118.94256 2118.93978 1.31 0.00093 1006956.9375 CID (Collision Induced Dissociation) MS2 26.34415 60 160.2 60.0 55.2586 15963 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 166.9 156.4 138.0 165.2 142.1 152.1 192.1 173.6 166.7 148.4 Excluded by Method 2.58 1 0.002079 0.008964 False High Sequest HT (A2) Unambiguous TFNSPNLK [K].tFNsPNLk.[D] N-Term(TMT6plex); S4(Phospho); K8(TMT6plex) 5 Q9Y6N7 Q9Y6N7; Q9Y6N7-4; Q9Y6N7-5; Q9Y6N7-3; Q9Y6N7-2 0 2 0.3732 0 1 1 729.89138 1458.77548 1458.7758 -0.22 -0.00016 3015580.25 CID (Collision Induced Dissociation) MS2 3.389981 10 144.7 59.541 77.1698 24101 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 141.9 146.7 114.4 141.6 138.0 140.3 153.0 160.3 143.7 167.0 Excluded by Method 3.51 1 0.002079 0.01082 False High Sequest HT (A2) Unambiguous RESVASGDDRAEEDMDEAIEK [K].rEsVASGDDRAEEDmDEAIEk.[G] N-Term(TMT6plex); S3(Phospho); M15(Oxidation); K21(TMT6plex) 1 P46821 P46821 2 4 0.1524 0 1 1 727.33364 2906.31275 2906.3127 0.02 1e-05 620192.4375 CID (Collision Induced Dissociation) MS2 0.0 60 11.9 60.0 56.2497 16330 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 11.0 14.5 4.0 9.3 16.1 13.7 12.5 13.0 12.3 12.8 Excluded by Method 2.69 1 0.002079 0.01036 False High Sequest HT (A2) Unambiguous TDEERPPVEHSPEK [K].tDEERPPVEHsPEk.[Q] N-Term(TMT6plex); S11(Phospho); K14(TMT6plex) 1 Q15170 Q15170 0 3 0.3552 0 1 1 730.02826 2188.07022 2188.06875 0.67 0.00049 977778.0625 CID (Collision Induced Dissociation) MS2 0.0 10 45.0 60.0 43.8994 11773 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 41.7 39.4 36.3 48.2 46.7 46.9 49.0 47.0 50.2 44.1 Excluded by Method 2.59 1 0.002079 0.01072 False High Sequest HT (A2) Unambiguous IVVVRAEELNISGEFFRNK [K].iVVVRAEELNISGEFFRNk.[L] N-Term(TMT6plex); K19(TMT6plex) 1 P26784 P26784 2 4 0.3909 0 1 1 670.39169 2678.54492 2678.53939 2.07 0.00138 512908.65625 CID (Collision Induced Dissociation) MS2 0.0 40 13.5 60.0 120.4376 40365 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 6.7 11.4 18.2 2.4 14.1 25.9 3.6 11.8 25.6 15.5 Excluded by Method 2.43 1 0.002079 0.009018 False High Sequest HT (A2) Unambiguous NEATPEAEQVKK [K].nEAtPEAEQVkk.[E] N-Term(TMT6plex); T4(Phospho); K11(TMT6plex); K12(TMT6plex) 1 Q08245 Q08245 1 3 0.5248 0 1 1 704.38329 2111.13532 2111.13526 0.03 2e-05 1453155.625 CID (Collision Induced Dissociation) MS2 11.72758 0 172.5 60.0 55.0992 15903 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 39.3 132.5 269.4 54.8 143.6 312.2 58.6 157.5 369.3 187.4 Excluded by Method 3.43 1 0.002079 0.009613 False High Sequest HT (A2) Unambiguous DENERASVSPMDEPVPDSESPIEK [K].dENERASVsPmDEPVPDSESPIEk.[V] N-Term(TMT6plex); S9(Phospho); M11(Oxidation); K24(TMT6plex) 1 P46821 P46821 1 4 0.1013 0 1 1 803.62519 3211.47894 3211.47541 1.1 0.00088 387451.71875 CID (Collision Induced Dissociation) MS2 6.704738 30 83.1 60.0 76.8461 23979 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 81.2 89.5 65.8 92.1 76.2 86.0 88.5 90.3 75.0 86.4 Excluded by Method 3.85 1 0.002079 0.01014 False High Sequest HT (A2) Unambiguous VQISPDSGGLPERSVSLTGAPESVQK [K].vQISPDSGGLPERSVsLTGAPESVQk.[A] N-Term(TMT6plex); S16(Phospho); K26(TMT6plex) 1 Q92945 Q92945 1 4 0.0199 0 1 1 794.9208 3176.66137 3176.66044 0.29 0.00023 845998.0 CID (Collision Induced Dissociation) MS2 67.80998 50 39.1 55.984 101.7477 33335 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 30.1 36.1 34.1 39.7 41.8 42.3 44.7 43.2 44.2 34.9 Excluded by Method 2.51 1 0.002079 0.01209 False High Sequest HT (A2) Unambiguous TMFAQVESDDEEAK [K].tmFAQVEsDDEEAk.[N] N-Term(TMT6plex); M2(Oxidation); S8(Phospho); K14(TMT6plex) 1 Q9NW82 Q9NW82 0 2 0.248 0 1 1 1077.48867 2153.97006 2153.9714 -0.62 -0.00067 345957.90625 CID (Collision Induced Dissociation) MS2 0.0 20 66.8 60.0 73.976 22921 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 65.1 60.8 51.9 69.2 69.7 62.3 83.5 69.6 64.7 70.8 Excluded by Method 2.5 1 0.002079 0.01282 False High Sequest HT (A2) Unambiguous QRSAGSFK [K].qRSAGsFk.[R] N-Term(TMT6plex); S6(Phospho); K8(TMT6plex) 3 P21359 P21359-2; P21359-6; P21359 1 3 0.3911 0 1 1 473.59008 1418.75568 1418.75574 -0.04 -2e-05 1378973.75 CID (Collision Induced Dissociation) MS2 34.21455 20 18.9 60.0 41.8991 11046 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 22.2 17.6 18.6 14.6 19.1 18.5 20.1 19.3 18.2 20.3 Excluded by Method 2.25 1 0.002079 0.009372 False High Sequest HT (A2) Unambiguous RSRLTPVSPESSSTEEK [K].rSRLtPVSPESSsTEEk.[S] N-Term(TMT6plex); T5(Phospho); S13(Phospho); K17(TMT6plex) 2 Q13501 Q13501-2; Q13501 2 4 0.0606 0 1 1 627.80917 2508.21483 2508.21484 0.0 0.0 1040962.9375 CID (Collision Induced Dissociation) MS2 6.566016 10 51.1 60.0 54.7837 15723 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 46.7 47.0 39.7 49.7 51.4 54.7 55.2 55.3 54.5 56.7 Excluded by Method 2.64 1 0.002079 0.008796 False High Sequest HT (A2) Unambiguous AVLFCLSEDK [K].aVLFcLSEDk.[K] N-Term(TMT6plex); C5(Carbamidomethyl); K10(TMT6plex) 1 P23528 P23528 0 2 0.4365 0 1 1 820.46203 1639.91677 1639.91295 2.33 0.00191 1419481.0 CID (Collision Induced Dissociation) MS2 15.70055 0 67.3 60.0 110.2142 36567 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 33.8 51.8 91.9 41.7 59.2 115.6 39.9 66.6 110.2 62.7 Excluded by Method 3.07 1 0.002079 0.008747 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVssDEEF.[-] N-Term(TMT6plex); S8(Phospho); S9(Phospho) 1 P16521 P16521 0 2 0.2109 0 1 1 925.35483 1849.70238 1849.70195 0.23 0.00021 1107308.75 CID (Collision Induced Dissociation) MS2 51.672 50 33.6 48.648 131.4203 44669 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 9.1 27.2 51.7 12.1 25.8 64.5 13.0 29.5 66.4 36.7 Excluded by Method 2.56 1 0.002079 0.008927 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVssDEEF.[-] N-Term(TMT6plex); S8(Phospho); S9(Phospho) 1 P16521 P16521 0 2 0.1856 0 1 1 925.35289 1849.69849 1849.70195 -1.87 -0.00173 492744.75 CID (Collision Induced Dissociation) MS2 21.18363 50 55.3 60.0 136.9166 46698 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 14.4 44.6 92.9 17.2 43.2 106.1 19.0 50.7 106.0 58.4 Excluded by Method 2.64 1 0.002079 0.01154 False High Sequest HT (A2) Unambiguous EEEEAKEESDDDMGFGLFD [K].eEEEAkEEsDDDmGFGLFD.[-] N-Term(TMT6plex); K6(TMT6plex); S9(Phospho); M13(Oxidation) 1 P05318 P05318 1 3 0.5268 0 1 1 916.0526 2746.14325 2746.13669 2.39 0.00219 786368.8125 CID (Collision Induced Dissociation) MS2 57.17322 40 144.7 60.0 118.0744 39400 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 35.0 111.7 201.9 46.9 113.7 246.3 50.4 134.5 346.4 160.0 Excluded by Method 3.55 1 0.002079 0.009559 False High Sequest HT (A2) Unambiguous DSTLIMQLLRDNLTLWTSENQGDEGDAGEGEN [K].dSTLImQLLRDNLTLWTSENQGDEGDAGEGEN.[-] N-Term(TMT6plex); M6(Oxidation) 2 P31946 P31946-2; P31946 1 4 0.4865 0 1 1 942.44948 3766.7761 3766.75512 5.57 0.00524 310678.3125 CID (Collision Induced Dissociation) MS2 29.75529 80 7.8 60.0 135.9366 46358 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 7.4 8.3 10.4 2.8 3.5 9.9 3.5 12.3 9.3 10.5 3.33 1 0.002079 0.01002 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVsSDEEF.[-] N-Term(TMT6plex); S8(Phospho) 1 P16521 P16521 0 2 0.0112 0 1 1 885.37113 1769.73498 1769.73562 -0.36 -0.00032 287932.3125 CID (Collision Induced Dissociation) MS2 16.36745 50 36.7 60.0 138.7511 47328 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 10.2 32.4 61.6 12.9 30.6 65.6 12.5 34.7 75.4 31.1 Excluded by Method 2.67 1 0.002079 0.01085 False High Sequest HT (A2) Unambiguous INSSPNVNTTASGVEDLNIIQVTIPDDDNERLSK [K].iNSsPNVNTTASGVEDLNIIQVTIPDDDNERLSk.[V] N-Term(TMT6plex); S4(Phospho); K34(TMT6plex) 4 P78347 P78347-3; P78347-4; P78347; P78347-2 1 4 0.0027 0 1 1 1052.53558 4207.12048 4207.11716 0.79 0.00083 3077539.75 CID (Collision Induced Dissociation) MS2 9.716143 60 4.3 15.683 127.6602 43007 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 3.2 2.6 2.1 1.6 6.0 4.1 7.3 6.5 7.1 2.6 Excluded by Method 3.75 1 0.002079 0.01142 False High Sequest HT (A2) Unambiguous GYGFVDFDSPSAAQK [K].gYGFVDFDsPSAAQk.[A] N-Term(TMT6plex); S9(Phospho); K15(TMT6plex) 1 Q15434 Q15434 0 3 0.0979 0 1 1 709.67892 2127.02221 2127.02001 1.03 0.00073 955281.375 CID (Collision Induced Dissociation) MS2 9.346675 40 118.5 60.0 109.8228 36384 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 112.0 121.9 100.9 111.8 112.6 113.0 137.3 128.0 133.3 114.2 Excluded by Method 3.88 1 0.002079 0.01106 False High Sequest HT (A2) Unambiguous EQAEASIDNLK [K].eQAEAsIDNLk.[N] N-Term(TMT6plex); S6(Phospho); K11(TMT6plex) 1 Q08245 Q08245 0 3 0.1485 0 1 1 585.96937 1755.89355 1755.89302 0.31 0.00018 2047496.75 CID (Collision Induced Dissociation) MS2 4.852967 50 153.5 51.673 76.3554 23823 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 44.4 113.4 240.6 66.6 145.3 246.6 70.2 161.9 288.8 157.1 Excluded by Method 2.02 1 0.002079 0.009986 False High Sequest HT (A2) Unambiguous VPVPGPGSGGNGPEREGPEEPGLAK [K].vPVPGPGsGGNGPEREGPEEPGLAk.[Q] N-Term(TMT6plex); S8(Phospho); K25(TMT6plex) 1 Q6ZRV2 Q6ZRV2 1 4 0.2141 0 1 1 731.3761 2922.48255 2922.47627 2.15 0.00157 572605.125 CID (Collision Induced Dissociation) MS2 31.8068 50 13.8 57.208 85.7315 27343 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 9.3 12.8 11.4 16.4 14.7 11.5 15.0 13.6 15.6 18.1 Excluded by Method 3.27 1 0.002079 0.008895 False High Sequest HT (A2) Unambiguous EECPRPMSISPPDFSPK [K].eEcPRPmSISPPDFsPk.[T] N-Term(TMT6plex); C3(Carbamidomethyl); M7(Oxidation); S15(Phospho); K17(TMT6plex) 1 P46821 P46821 0 3 0.2626 0 1 1 843.40463 2528.19933 2528.19667 1.05 0.00089 405784.0 CID (Collision Induced Dissociation) MS2 0.0 30 59.9 60.0 84.9558 27113 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 60.5 64.6 46.5 72.8 56.4 54.6 69.7 57.6 57.1 59.7 Excluded by Method 2.78 1 0.002079 0.01109 False High Sequest HT (A2) Unambiguous SESPCESPYPNEK [K].sESPcESPYPNEk.[D] N-Term(TMT6plex); S1(Phospho); C5(Carbamidomethyl); K13(TMT6plex) 2 Q8NI27 Q8NI27; Q8NI27-2 0 3 0.0307 0 1 1 687.97955 2061.92409 2061.92406 0.01 1e-05 3577673.75 CID (Collision Induced Dissociation) MS2 5.763303 60 16.4 22.783 59.5038 17504 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 18.1 14.0 15.4 17.0 15.3 20.5 15.3 15.2 15.4 17.8 Excluded by Method 2.61 1 0.002079 0.01239 False High Sequest HT (A2) Unambiguous LEAGLSDSK [K].lEAGLsDSk.[Q] N-Term(TMT6plex); S6(Phospho); K9(TMT6plex) 1 P17536 P17536 0 2 0.2771 0 1 1 729.38647 1457.76567 1457.7653 0.26 0.00019 1519916.875 CID (Collision Induced Dissociation) MS2 35.66768 40 61.9 60.0 76.8266 24005 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 22.3 46.3 94.9 21.7 49.8 121.2 30.1 62.7 107.0 62.9 Excluded by Method 3.32 1 0.002079 0.01054 False High Sequest HT (A2) Unambiguous DESDSENDIEIK [K].dESDsENDIEIk.[G] N-Term(TMT6plex); S5(Phospho); K12(TMT6plex) 1 P32499 P32499 0 3 0.1755 0 1 1 644.63378 1931.88679 1931.88872 -1.0 -0.00064 520040.5625 CID (Collision Induced Dissociation) MS2 0.0 60 19.5 60.0 75.8088 23666 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 15.4 21.5 21.2 14.0 15.9 23.5 16.5 20.0 29.7 17.4 Excluded by Method 2.45 1 0.002079 0.01099 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVSsDEEF.[-] N-Term(TMT6plex); S9(Phospho) 1 P16521 P16521 0 2 0.025 0 1 1 885.37101 1769.73475 1769.73562 -0.49 -0.00044 400753.75 CID (Collision Induced Dissociation) MS2 36.16844 40 61.4 60.0 121.2666 40585 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 15.4 48.3 101.0 18.7 46.0 119.3 24.0 59.0 117.2 65.2 Excluded by Method 2.4 1 0.002079 0.01182 False High Sequest HT (A2) Unambiguous DMSPLSETEMALGK [K].dmSPLsETEmALGk.[D] N-Term(TMT6plex); M2(Oxidation); S6(Phospho); M10(Oxidation); K14(TMT6plex) 3 P27816 P27816; P27816-6; P27816-2 0 3 0.0262 0 1 1 693.66466 2078.97942 2078.97913 0.14 0.0001 721022.875 CID (Collision Induced Dissociation) MS2 8.236831 50 43.3 60.0 109.2165 36156 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 42.1 38.0 40.3 45.1 41.3 39.8 52.2 46.8 46.2 41.2 Excluded by Method 2.29 1 0.002079 0.0119 False High Sequest HT (A2) Unambiguous SPSLSPSPPSPLEK [K].sPSLSPSPPSPLEk.[T] N-Term(TMT6plex); S1(Phospho); K14(TMT6plex) 1 P46821 P46821 0 3 0.0131 0 1 1 654.35142 1961.03971 1961.03968 0.02 1e-05 1979551.25 CID (Collision Induced Dissociation) MS2 17.70449 30 110.2 43.222 91.9873 29753 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 105.4 101.9 83.5 111.8 107.8 109.9 128.6 134.4 111.2 107.6 Excluded by Method 3.82 1 0.002079 0.008989 False High Sequest HT (A2) Unambiguous SPSLSPSPPSPLEK [K].sPsLsPsPPSPLEk.[T] N-Term(TMT6plex); S3(Phospho); S5(Phospho); S7(Phospho); K14(TMT6plex) 1 P46821 P46821 0 3 0.1226 0 1 1 707.66252 2120.97302 2120.97234 0.32 0.00022 3457438.25 CID (Collision Induced Dissociation) MS2 1.683891 30 95.1 60.0 97.9625 32003 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 105.5 95.8 76.4 101.7 92.0 98.5 106.0 97.6 92.8 84.6 Excluded by Method 3.18 1 0.002079 0.0117 False High Sequest HT (A2) Unambiguous NAEEESESEAEEGD [K].nAEEEsEsEAEEGD.[-] N-Term(TMT6plex); S6(Phospho); S8(Phospho) 4 Q7L1Q6 Q7L1Q6-2; Q7L1Q6-4; Q7L1Q6-3; Q7L1Q6 0 2 0.9762 0 1 1 957.3245 1913.64172 1913.6412 0.27 0.00026 101669.7265625 CID (Collision Induced Dissociation) MS2 0.0 50 4.7 60.0 44.3814 11903 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 3.0 6.4 3.9 8.2 3.1 2.9 2.3 3.0 7.6 6.3 Excluded by Method 2.1 1 0.002079 0.009382 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVSsDEEF.[-] N-Term(TMT6plex); S9(Phospho) 1 P16521 P16521 0 2 0.0829 0 1 1 885.3715 1769.73573 1769.73562 0.06 5e-05 39000344.0 CID (Collision Induced Dissociation) MS2 41.46295 10 41.4 60.0 112.0478 37251 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 15.5 28.5 60.1 22.0 34.8 66.0 23.9 41.5 74.9 46.4 Excluded by Method 2.17 1 0.002079 0.01202 False High Sequest HT (A2) Unambiguous MPSFGVSAPGK [K].mPsFGVSAPGk.[S] N-Term(TMT6plex); S3(Phospho); K11(TMT6plex) 2 Q8IVF2 Q8IVF2-3; Q8IVF2 0 3 0.363 0 1 1 539.28224 1615.83216 1615.83194 0.14 7e-05 558458.125 CID (Collision Induced Dissociation) MS2 42.29965 60 29.1 60.0 103.6509 34082 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 24.0 29.6 27.4 23.2 29.7 36.6 31.6 27.8 32.5 28.8 Excluded by Method 2.92 1 0.002079 0.01203 False High Sequest HT (A2) Unambiguous KETERASPIK [LK].kETERAsPIk.[M] N-Term(TMT6plex); K1(TMT6plex); S7(Phospho); K10(TMT6plex) 3 P27816 P27816; P27816-6; P27816-2 2 4 0.2477 0 1 1 482.28141 1926.10383 1926.10284 0.51 0.00025 1174752.625 CID (Collision Induced Dissociation) MS2 3.803747 30 218.7 60.0 45.8329 12433 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 199.9 221.0 169.9 236.2 211.1 219.5 245.1 236.1 211.9 236.3 Excluded by Method 2.18 1 0.002079 0.009672 False High Sequest HT (A2) Unambiguous LSGFSFK [K].lSGFsFk.[K] N-Term(TMT6plex); S5(Phospho); K7(TMT6plex) 1 P29966 P29966 0 2 0.2426 0 1 1 662.35953 1323.71179 1323.71141 0.28 0.00019 6305675.5 CID (Collision Induced Dissociation) MS2 2.83756 10 175.3 27.743 112.2081 37311 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 203.2 176.0 165.2 187.9 159.8 158.2 204.7 186.4 157.5 153.9 Excluded by Method 2.02 1 0.002079 0.01032 False High Sequest HT (A2) Unambiguous DDGSDRDSDREQDEK [K].dDGsDRDsDREQDEk.[Q] N-Term(TMT6plex); S4(Phospho); S8(Phospho); K15(TMT6plex) 1 Q9UGP8 Q9UGP8 2 3 0.5331 0 1 1 795.66085 2384.968 2384.96449 1.47 0.00117 72516.359375 CID (Collision Induced Dissociation) MS2 37.40996 50 35.0 60.0 29.7488 6675 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 35.0 37.2 28.6 38.3 29.7 28.6 44.1 43.8 29.4 35.5 Excluded by Method 2.72 1 0.002079 0.01081 False High Sequest HT (A2) Unambiguous SLTAAFYGDK [K].sLTAAFYGDk.[Q] N-Term(TMT6plex); S1(Phospho); K10(TMT6plex) 5 Q2LD37 Q2LD37-4; Q2LD37-6; Q2LD37; Q2LD37-2; Q2LD37-7 0 3 0.1724 0 1 1 537.61195 1610.8213 1610.82315 -1.15 -0.00062 493680.5625 CID (Collision Induced Dissociation) MS2 0.0 50 12.4 60.0 103.4744 34016 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 12.6 9.7 10.9 10.6 12.5 16.0 14.6 11.3 12.5 13.2 Excluded by Method 2.03 1 0.002079 0.009158 False High Sequest HT (A2) Unambiguous LRPRSIEVENDFLPVEK [K].lRPRsIEVENDFLPVEk.[T] N-Term(TMT6plex); S5(Phospho); K17(TMT6plex) 2 Q96B97 Q96B97-2; Q96B97 1 4 0.1845 0 1 1 645.60576 2579.40122 2579.39986 0.53 0.00034 954975.1875 CID (Collision Induced Dissociation) MS2 4.528862 50 93.3 60.0 103.8964 34174 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 100.4 84.8 87.3 96.2 88.2 90.1 104.1 102.5 90.6 89.1 Excluded by Method 2.06 1 0.002079 0.008633 False High Sequest HT (A2) Unambiguous NNDGEEQEVRDDNRPK [K].nNDGEEQEVRDDNRPk.[G] N-Term(TMT6plex); K16(TMT6plex) 1 O00461 O00461 1 4 0.3511 0 1 1 594.0505 2373.18016 2373.1795 0.28 0.00017 70502.8828125 CID (Collision Induced Dissociation) MS2 9.616012 70 6.2 60.0 34.4843 8332 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 7.6 8.5 3.2 3.0 2.2 4.0 10.0 7.4 9.2 6.9 2.25 1 0.002079 0.01254 False High Sequest HT (A2) Unambiguous SPSLSPSPPSPLEK [K].sPSLSPsPPsPLEk.[T] N-Term(TMT6plex); S7(Phospho); S10(Phospho); K14(TMT6plex) 1 P46821 P46821 0 3 0.2531 0 1 1 681.00691 2041.00618 2041.00601 0.08 6e-05 1476187.25 CID (Collision Induced Dissociation) MS2 3.625881 20 150.3 44.013 95.1471 30937 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 144.8 150.7 121.8 167.5 138.9 139.9 182.4 160.7 142.8 154.0 Excluded by Method 3.2 1 0.002079 0.01286 False High Sequest HT (A2) Unambiguous HDGGSPSIK [K].hDGGsPSIk.[A] N-Term(TMT6plex); S5(Phospho); K9(TMT6plex) 1 Q15648 Q15648 0 3 0.488 0 1 1 479.24983 1435.73495 1435.73467 0.2 9e-05 534934.75 CID (Collision Induced Dissociation) MS2 19.59721 40 31.7 60.0 40.4965 10500 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 29.3 32.9 27.0 28.0 29.1 32.9 36.0 34.6 34.5 32.5 Excluded by Method 2.5 1 0.002079 0.01005 False High Sequest HT (A2) Unambiguous NAEEESESEAEEGD [K].nAEEEsEsEAEEGD.[-] N-Term(TMT6plex); S6(Phospho); S8(Phospho) 4 Q7L1Q6 Q7L1Q6-2; Q7L1Q6-4; Q7L1Q6-3; Q7L1Q6 0 2 0.9033 0 1 1 957.3237 1913.64013 1913.6412 -0.56 -0.00054 278654.0625 CID (Collision Induced Dissociation) MS2 0.0 40 12.9 60.0 41.6359 10908 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 12.9 13.9 9.6 11.7 12.4 14.5 15.2 13.4 12.7 12.5 Excluded by Method 2.69 1 0.002079 0.009975 False High Sequest HT (A2) Unambiguous TASESISNLSEAGSIK [K].tASESISNLSEAGsIk.[K] N-Term(TMT6plex); S14(Phospho); K16(TMT6plex) 3 P30622 P30622-2; P30622; P30622-1 0 3 0.0714 0 1 1 711.36794 2132.08928 2132.08882 0.22 0.00015 1908632.125 CID (Collision Induced Dissociation) MS2 4.47627 30 27.4 60.0 88.0626 28212 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 23.3 28.6 24.8 28.5 27.1 27.2 28.1 27.1 33.7 25.6 Excluded by Method 1.82 1 0.002079 0.00951 False High Sequest HT (A2) Unambiguous DLSIEDQIK [K].dLsIEDQIk.[K] N-Term(TMT6plex); S3(Phospho); K9(TMT6plex) 1 P53072 P53072 0 2 0.3022 0 1 1 799.92604 1598.8448 1598.84428 0.33 0.00026 1140928.625 CID (Collision Induced Dissociation) MS2 29.72436 10 38.4 60.0 95.9312 31235 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 27.2 43.9 39.2 28.8 33.2 51.2 32.8 44.3 51.2 31.9 Excluded by Method 2.78 1 0.002079 0.009846 False High Sequest HT (A2) Unambiguous GTVQQADE [K].gTVQQADE.[-] N-Term(TMT6plex) 1 P32969 P32969 0 2 0.599 0 1 1 538.77478 1076.54228 1076.54213 0.14 8e-05 183402.484375 CID (Collision Induced Dissociation) MS2 0.0 80 17.1 60.0 29.5233 6596 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 17.2 17.4 11.2 17.3 16.8 20.0 21.4 18.1 15.0 16.9 1.92 1 0.002079 0.009301 False High Sequest HT (A2) Unambiguous EPETRYSVLNNDDYFADVSPLRATSPSK [K].ePETRYSVLNNDDYFADVsPLRATsPSk.[S] N-Term(TMT6plex); S19(Phospho); S25(Phospho); K28(TMT6plex) 1 Q1ED39 Q1ED39 2 4 0.3061 0 1 1 948.20054 3789.78032 3789.78144 -0.29 -0.00028 1174785.5 CID (Collision Induced Dissociation) MS2 48.18777 30 38.0 58.915 112.9189 37576 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 38.9 38.6 30.8 36.6 39.8 38.8 42.5 37.3 40.8 36.4 Excluded by Method 2.94 1 0.002079 0.009904 False High Sequest HT (A2) Unambiguous GADFLVTEVENGGSLGSK [K].gADFLVTEVENGGSLGSk.[K] N-Term(TMT6plex); K18(TMT6plex) 3 P14618 P14618-2; P14618-3; P14618 0 3 0.3368 0 1 1 746.73882 2238.20191 2238.2018 0.05 4e-05 975232.5625 CID (Collision Induced Dissociation) MS2 11.94589 40 16.7 60.0 118.6739 39624 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 18.2 17.2 18.3 15.9 8.0 23.4 15.9 16.8 17.9 15.1 Excluded by Method 1.93 1 0.002079 0.01072 False High Sequest HT (A2) Unambiguous DTSTSADAEASEDQNK [K].dTStSADAEASEDQNk.[E] N-Term(TMT6plex); T4(Phospho); K16(TMT6plex) 1 P53885 P53885 0 3 0.0045 0 1 1 736.33095 2206.9783 2206.9753 1.36 0.001 375394.28125 CID (Collision Induced Dissociation) MS2 0.0 90 8.9 60.0 45.8892 12495 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 4.0 7.9 11.5 7.6 15.7 2.5 8.1 18.9 12.5 2.22 1 0.002079 0.01119 False High Sequest HT (A2) Unambiguous AGMQPGLTMVVGPPGTGK [R].aGmQPGLtMVVGPPGTGk.[T] N-Term(TMT6plex); M3(Oxidation); T8(Phospho); K18(TMT6plex) 1 O60306 O60306 0 3 0.1268 0 1 1 751.39234 2252.16245 2252.15843 1.78 0.00134 436944.625 CID (Collision Induced Dissociation) MS2 62.56237 40 27.3 60.0 78.3739 24636 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 23.2 24.7 21.3 26.6 24.7 27.8 32.8 31.8 34.4 25.8 Excluded by Method 2.13 1 0.002079 0.01298 False High Sequest HT (A2) Unambiguous GGSSLSPDK [K].gGSSLsPDk.[S] N-Term(TMT6plex); S6(Phospho); K9(TMT6plex) 1 Q12044 Q12044 0 3 0.2246 0 1 1 462.57414 1385.70788 1385.70778 0.07 3e-05 578918.4375 CID (Collision Induced Dissociation) MS2 43.62756 60 21.2 60.0 51.798 14624 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 12.6 14.6 29.4 9.4 19.5 36.9 11.9 16.3 42.0 19.8 Excluded by Method 2.36 1 0.002079 0.01022 False High Sequest HT (A2) Unambiguous SPTSPLNMK [K].sPTsPLNMk.[L] N-Term(TMT6plex); S4(Phospho); K9(TMT6plex) 2 P28370 P28370-2; P28370 0 3 0.0779 0 1 1 504.93468 1512.78948 1512.78974 -0.17 -8e-05 620502.5 CID (Collision Induced Dissociation) MS2 19.6096 30 63.1 60.0 81.371 25763 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 65.9 62.7 57.2 56.7 49.3 66.1 78.0 62.9 67.5 65.0 Excluded by Method 2.44 1 0.002079 0.0127 False High Sequest HT (A2) Unambiguous EPPLTPVPLK [K].ePPLtPVPLk.[R] N-Term(TMT6plex); T5(Phospho); K10(TMT6plex) 1 P09884 P09884 0 3 0.363 0 1 1 543.6522 1628.94204 1628.94287 -0.51 -0.00028 317436.96875 CID (Collision Induced Dissociation) MS2 8.294348 50 73.1 60.0 97.7049 31902 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 47.0 62.5 88.0 50.6 66.4 107.8 58.0 73.5 100.9 76.4 Excluded by Method 2.7 1 0.002079 0.009728 False High Sequest HT (A2) Unambiguous EEEAKEESDDDMGFGLFD [K].eEEAkEEsDDDmGFGLFD.[-] N-Term(TMT6plex); K5(TMT6plex); S8(Phospho); M12(Oxidation) 1 P02400 P02400 1 3 0.4979 0 1 1 873.03742 2617.0977 2617.0941 1.38 0.0012 1047261.625 CID (Collision Induced Dissociation) MS2 0.0 40 148.9 60.0 120.629 40350 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 35.3 113.8 220.1 51.6 133.1 267.4 51.7 129.4 325.1 161.0 Excluded by Method 2.41 1 0.002079 0.01017 False High Sequest HT (A2) Unambiguous KETQSPEQVK [K].kETQsPEQVk.[S] N-Term(TMT6plex); K1(TMT6plex); S5(Phospho); K10(TMT6plex) 3 Q9NYF8 Q9NYF8-3; Q9NYF8; Q9NYF8-2 1 4 0.006 0 1 1 486.02228 1941.0673 1941.06612 0.61 0.0003 763435.6875 CID (Collision Induced Dissociation) MS2 0.0 30 86.8 60.0 49.1781 13657 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 83.5 81.9 67.1 91.2 94.8 75.9 109.6 91.2 88.5 84.4 Excluded by Method 3.35 1 0.002079 0.0115 False High Sequest HT (A2) Unambiguous NFHAEVSTPQVLSAK [K].nFHAEVStPQVLSAk.[K] N-Term(TMT6plex); T8(Phospho); K15(TMT6plex) 1 P48570 P48570 0 3 0.0649 0 1 1 722.71943 2166.14374 2166.13604 3.55 0.00256 892984.1875 CID (Collision Induced Dissociation) MS2 39.85956 50 66.3 60.0 87.4441 27989 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 31.3 60.9 90.9 38.9 64.6 98.2 42.6 68.6 97.9 68.9 Excluded by Method 3.7 1 0.002079 0.00902 False High Sequest HT (A2) Unambiguous EPSRERDIAK [K].ePsRERDIAk.[E] N-Term(TMT6plex); S3(Phospho); K10(TMT6plex) 2 Q8NI27 Q8NI27; Q8NI27-2 2 3 0.0108 0 1 1 580.31329 1738.92532 1738.92532 0.0 0.0 6632480.5 CID (Collision Induced Dissociation) MS2 4.503582 20 60.7 30.28 41.4789 10896 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 50.5 63.5 46.9 66.9 54.9 65.8 65.1 67.8 60.8 64.8 Excluded by Method 1.85 1 0.002079 0.01072 False High Sequest HT (A2) Unambiguous RLLGDSDSEEEQK [K].rLLGDsDsEEEQk.[E] N-Term(TMT6plex); S6(Phospho); S8(Phospho); K13(TMT6plex) 1 Q9BW71 Q9BW71 1 3 0.551 0 1 1 708.66085 2123.96801 2123.96633 0.79 0.00056 558541.5625 CID (Collision Induced Dissociation) MS2 13.97115 20 140.0 60.0 70.1742 21492 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 132.5 124.5 112.8 155.7 127.3 135.2 149.8 153.3 156.6 152.0 Excluded by Method 3.14 1 0.002079 0.009364 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVsSDEEF.[-] N-Term(TMT6plex); S8(Phospho) 1 P16521 P16521 0 2 0.0239 0 1 1 885.37181 1769.73634 1769.73562 0.4 0.00036 608838.8125 CID (Collision Induced Dissociation) MS2 41.02452 50 65.9 60.0 132.8103 45239 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 17.4 50.8 101.1 17.6 46.5 132.7 33.4 64.3 133.4 61.4 Excluded by Method 2.93 1 0.002079 0.0107 False High Sequest HT (A2) Unambiguous SALSPSKSPAK [K].sALSPSksPAk.[L] N-Term(TMT6plex); K7(TMT6plex); S8(Phospho); K11(TMT6plex) 2 P49792 P49792; Q7Z3J3 1 3 0.0732 0 1 1 614.02298 1840.05439 1840.05483 -0.24 -0.00015 607997.25 CID (Collision Induced Dissociation) MS2 5.737104 10 76.1 60.0 57.5624 16788 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 74.0 80.1 55.5 91.0 76.1 73.7 85.6 81.3 65.1 78.3 Excluded by Method 2.05 1 0.002079 0.01034 False High Sequest HT (A2) Unambiguous STESLQANVQRLK [K].sTESLQANVQRLk.[E] N-Term(TMT6plex); S1(Phospho); K13(TMT6plex) 1 P26373 P26373 1 3 0.166 0 1 1 671.36959 2012.09422 2012.09418 0.02 2e-05 1615075.0 CID (Collision Induced Dissociation) MS2 0.0 20 22.8 60.0 84.2669 26860 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 21.9 18.5 21.9 21.2 19.9 25.4 23.6 26.8 24.0 24.6 Excluded by Method 2.59 1 0.002079 0.01113 False High Sequest HT (A2) Unambiguous EEEAKEESDDDMGFGLFD [K].eEEAkEEsDDDmGFGLFD.[-] N-Term(TMT6plex); K5(TMT6plex); S8(Phospho); M12(Oxidation) 1 P02400 P02400 1 3 0.5942 0 1 1 873.03582 2617.0929 2617.0941 -0.46 -0.0004 2040475.75 CID (Collision Induced Dissociation) MS2 6.598768 20 111.1 44.382 118.1991 39487 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 28.5 88.8 163.0 38.4 92.5 206.7 39.6 107.6 237.7 108.0 Excluded by Method 3.45 1 0.002079 0.009225 False High Sequest HT (A2) Unambiguous NTGVSPASRPSPGTPTSPSNLTSGLK [K].nTGVSPASRPSPGTPTSPsNLTSGLk.[T] N-Term(TMT6plex); S19(Phospho); K26(TMT6plex) 4 Q5VT52 Q5VT52-5; Q5VT52; Q5VT52-2; Q5VT52-3 0 4 0.0133 0 1 1 762.90077 3048.58126 3048.57671 1.49 0.00114 888695.5625 CID (Collision Induced Dissociation) MS2 25.08424 60 45.8 60.0 83.2524 26421 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 49.1 46.1 41.6 54.0 46.4 47.0 49.8 42.8 42.3 38.7 Excluded by Method 3.76 1 0.002079 0.01106 False High Sequest HT (A2) Unambiguous FLNIENPENQSE [K].fLNIENPENQsE.[-] N-Term(TMT6plex); S11(Phospho) 1 Q04779 Q04779 0 3 0.443 0 1 1 581.59846 1742.78084 1742.78358 -1.57 -0.00091 142529.921875 CID (Collision Induced Dissociation) MS2 64.80708 40 44.6 60.0 117.889 39369 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 13.2 34.4 68.0 13.0 36.1 81.4 16.4 39.1 95.1 49.5 Excluded by Method 3.86 1 0.002079 0.01056 False High Sequest HT (A2) Unambiguous EEEAKEESDDDMGFGLFD [K].eEEAkEEsDDDmGFGLFD.[-] N-Term(TMT6plex); K5(TMT6plex); S8(Phospho); M12(Oxidation) 1 P02400 P02400 1 2 0.6185 0 1 1 1309.05341 2617.09955 2617.0941 2.08 0.00273 815878.375 CID (Collision Induced Dissociation) MS2 0.0 20 53.2 60.0 115.6249 38501 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 12.3 43.7 81.0 16.8 49.6 96.8 19.4 46.7 109.0 56.4 Excluded by Method 2.49 1 0.002079 0.008684 False High Sequest HT (A2) Unambiguous AAPAQVRPPSPGNIRPVK [K].aAPAQVRPPsPGNIRPVk.[R] N-Term(TMT6plex); S10(Phospho); K18(TMT6plex) 7 Q14244 Q14244-7; Q14244; Q14244-4; Q14244-3; Q14244-5; Q14244-6; Q14244-2 0 4 0.3768 0 1 1 599.09617 2393.36285 2393.35827 1.91 0.00115 404400.75 CID (Collision Induced Dissociation) MS2 26.35888 20 27.3 60.0 74.5679 23205 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 32.8 28.2 26.0 24.7 21.1 25.7 28.4 31.9 24.8 28.9 Excluded by Method 2.07 1 0.002079 0.00872 False High Sequest HT (A2) Unambiguous ALEEVSLK [K].aLEEVsLk.[W] N-Term(TMT6plex); S6(Phospho); K8(TMT6plex) 1 P14126 P14126 0 2 0.2887 0 1 1 713.90184 1426.79639 1426.79587 0.37 0.00026 1682759.375 CID (Collision Induced Dissociation) MS2 0.0 30 48.4 60.0 90.7025 29266 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 16.1 37.6 67.0 20.7 40.7 82.1 24.8 49.2 96.1 49.6 Excluded by Method 3.36 1 0.002079 0.008945 False High Sequest HT (A2) Unambiguous VFDDESDEKEDEEYADEK [K].vFDDEsDEkEDEEYADEk.[G] N-Term(TMT6plex); S6(Phospho); K9(TMT6plex); K18(TMT6plex) 1 O43719 O43719 1 4 0.2591 0 1 1 740.58742 2959.32783 2959.32247 1.81 0.00134 403719.4375 CID (Collision Induced Dissociation) MS2 56.19368 40 102.1 60.0 87.2069 27953 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 110.4 90.4 74.4 122.6 101.2 98.2 119.8 105.0 99.4 99.5 Excluded by Method 3.28 1 0.002079 0.01249 False High Sequest HT (A2) Unambiguous DYDTLSK [K].dYDTLsk.[C] N-Term(TMT6plex); S6(Phospho); K7(TMT6plex) 1 Q9UHR4 Q9UHR4 0 2 0.2182 0 1 1 690.34665 1379.68602 1379.68598 0.03 2e-05 719988.875 CID (Collision Induced Dissociation) MS2 0.0 20 46.3 60.0 67.9228 20633 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 48.0 45.0 38.5 49.0 52.1 39.8 55.4 38.7 46.9 49.1 Excluded by Method 2.75 1 0.002079 0.009553 False High Sequest HT (A2) Unambiguous FEGLAASEK [K].fEGLAAsEk.[E] N-Term(TMT6plex); S7(Phospho); K9(TMT6plex) 1 P15891 P15891 0 3 0.2915 0 1 1 497.26141 1489.76967 1489.77038 -0.48 -0.00024 293252.0 CID (Collision Induced Dissociation) MS2 57.65432 30 23.4 60.0 87.2673 27977 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 11.2 20.7 25.6 11.4 15.6 41.1 13.9 21.2 47.7 25.7 Excluded by Method 2.95 1 0.002079 0.008627 False High Sequest HT (A2) Unambiguous SQVDGAGDVSNEEIAADDTK [K].sQVDGAGDVsNEEIAADDTk.[K] N-Term(TMT6plex); S10(Phospho); K20(TMT6plex) 1 P53438 P53438 0 3 0.1729 0 1 1 853.73234 2559.18246 2559.18636 -1.52 -0.0013 651632.4375 CID (Collision Induced Dissociation) MS2 4.976156 40 19.2 60.0 73.9602 22888 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 7.5 14.7 24.4 8.5 17.2 27.5 10.2 19.7 40.0 22.7 Excluded by Method 2.14 1 0.002079 0.01089 False High Sequest HT (A2) Unambiguous IAISPLK [K].iAIsPLk.[S] N-Term(TMT6plex); S4(Phospho); K7(TMT6plex) 2 Q6MZP7 Q6MZP7-3; Q6MZP7 0 2 0.1004 0 1 1 640.39331 1279.77934 1279.7791 0.19 0.00012 1540002.125 CID (Collision Induced Dissociation) MS2 5.618755 20 52.6 60.0 100.6441 33006 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 53.9 58.1 41.1 65.0 48.1 47.7 51.2 51.8 57.0 51.8 Excluded by Method 2.59 1 0.002079 0.009339 False High Sequest HT (A2) Unambiguous DSISAVSSEKVSPSK [K].dSISAVSSEkVsPSk.[S] N-Term(TMT6plex); K10(TMT6plex); S12(Phospho); K15(TMT6plex) 1 P46821 P46821 1 4 0.1268 0 1 1 572.81372 2288.23305 2288.23537 -1.01 -0.00058 922200.875 CID (Collision Induced Dissociation) MS2 8.270401 30 33.3 60.0 73.6947 22783 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 28.3 30.9 36.6 29.3 32.6 37.5 27.8 37.2 38.5 34.0 Excluded by Method 2.76 1 0.002079 0.009318 False High Sequest HT (A2) Unambiguous IASPVSRKEPPLTPVPLK [K].iASPVsRkEPPLtPVPLk.[R] N-Term(TMT6plex); S6(Phospho); K8(TMT6plex); T13(Phospho); K18(TMT6plex) 1 P09884 P09884 2 4 0.052 0 1 1 694.89954 2776.57631 2776.57462 0.61 0.00042 365285.9375 CID (Collision Induced Dissociation) MS2 18.54278 40 91.8 60.0 100.7368 33040 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 99.1 93.9 71.0 98.8 85.4 94.1 93.8 92.6 96.9 92.7 Excluded by Method 2.69 1 0.002079 0.01066 False High Sequest HT (A2) Unambiguous KTSTSPPPEK [K].kTsTsPPPEk.[S] N-Term(TMT6plex); K1(TMT6plex); S3(Phospho); S5(Phospho); K10(TMT6plex) 2 Q9H1E3 Q9H1E3; Q9H1E3-2 1 3 0.0067 0 1 1 640.33508 1918.99069 1918.98952 0.61 0.00039 343735.40625 CID (Collision Induced Dissociation) MS2 23.04735 20 134.5 60.0 50.1145 14032 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 132.6 133.6 104.5 143.1 136.4 128.1 156.6 140.3 137.9 132.3 Excluded by Method 2.98 1 0.002079 0.00887 False High Sequest HT (A2) Unambiguous ASEAASPLPDSPGDK [K].aSEAASPLPDsPGDk.[L] N-Term(TMT6plex); S11(Phospho); K15(TMT6plex) 2 Q68CZ2 Q68CZ2; Q68CZ2-2 0 3 0.1827 0 1 1 660.663 1979.97445 1979.97272 0.87 0.00057 785655.75 CID (Collision Induced Dissociation) MS2 54.05221 60 42.5 60.0 72.0236 22248 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 44.0 40.0 39.0 43.1 49.1 40.8 44.6 41.2 43.7 39.9 Excluded by Method 1.97 1 0.002079 0.008953 False High Sequest HT (A2) Unambiguous LGFSSSPTK [K].lGFSSsPTk.[K] N-Term(TMT6plex); S6(Phospho); K9(TMT6plex) 3 Q8IVF2 Q8IVF2-3; Q8IVF2-2; Q8IVF2 0 3 0.1067 0 1 1 487.9301 1461.77575 1461.77547 0.19 0.0001 3105838.0 CID (Collision Induced Dissociation) MS2 5.493394 70 133.0 60.0 82.3733 26089 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 130.5 123.2 103.0 136.2 117.6 143.8 142.4 143.0 139.8 150.5 2.25 1 0.002079 0.01089 False High Sequest HT (A2) Unambiguous YAPVSPTVQQK [K].yAPVsPTVQQk.[Q] N-Term(TMT6plex); S5(Phospho); K11(TMT6plex) 1 P48415 P48415 0 3 0.0258 0 1 1 585.9865 1755.94496 1755.94466 0.17 0.0001 1903817.125 CID (Collision Induced Dissociation) MS2 45.31296 40 57.6 33.609 72.2523 22334 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 37.7 47.3 69.7 44.2 57.4 85.0 36.1 56.5 82.9 59.2 Excluded by Method 3.49 1 0.002079 0.01261 False High Sequest HT (A2) Unambiguous FYDDENLTE [K].fYDDENLtE.[-] N-Term(TMT6plex); T8(Phospho) 1 P50278 P50278 0 2 0.7057 0 1 1 727.80011 1454.59295 1454.59259 0.25 0.00018 38728168.0 CID (Collision Induced Dissociation) MS2 39.41178 40 140.2 3.016 94.1733 30520 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 61.4 115.1 186.6 64.4 112.1 246.0 78.9 126.1 260.3 151.2 Excluded by Method 2.82 1 0.002079 0.0115 False High Sequest HT (A2) Unambiguous EEEAKEESDDDMGFGLFD [K].eEEAkEEsDDDMGFGLFD.[-] N-Term(TMT6plex); K5(TMT6plex); S8(Phospho) 1 P02400 P02400 1 3 0.5172 0 1 1 867.70534 2601.10146 2601.09918 0.87 0.00076 1992938.125 CID (Collision Induced Dissociation) MS2 25.71707 20 94.7 22.381 127.7178 43007 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 31.1 80.2 126.9 34.8 74.5 165.9 43.4 101.0 191.2 97.7 Excluded by Method 2.9 1 0.002079 0.01078 False High Sequest HT (A2) Unambiguous NAEEESESEAEEGD [K].nAEEEsEsEAEEGD.[-] N-Term(TMT6plex); S6(Phospho); S8(Phospho) 4 Q7L1Q6 Q7L1Q6-2; Q7L1Q6-4; Q7L1Q6-3; Q7L1Q6 0 2 0.911 0 1 1 957.3251 1913.64293 1913.6412 0.9 0.00086 69337.59375 CID (Collision Induced Dissociation) MS2 0.0 60 3.6 60.0 44.2276 11890 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 2.7 3.7 1.5 3.8 6.4 3.9 3.4 4.0 3.4 3.1 Excluded by Method 1.91 1 0.002079 0.01176 False High Sequest HT (A2) Unambiguous DLPSLGSNAAFANVK [K].dLPSLGsNAAFANVk.[V] N-Term(TMT6plex); S7(Phospho); K15(TMT6plex) 1 P06105 P06105 0 3 0.1193 0 1 1 681.36164 2042.07035 2042.07238 -0.99 -0.00067 854630.9375 CID (Collision Induced Dissociation) MS2 0.0 100 82.7 60.0 115.5945 38525 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 59.1 78.1 81.5 66.2 87.5 105.8 71.2 79.4 109.5 88.4 3.27 1 0.002079 0.01057 False High Sequest HT (A2) Unambiguous TESPLENVAELK [K].tEsPLENVAELk.[K] N-Term(TMT6plex); S3(Phospho); K12(TMT6plex) 1 P35732 P35732 0 3 0.0109 0 1 1 623.33247 1867.98287 1867.98183 0.55 0.00034 581101.125 CID (Collision Induced Dissociation) MS2 3.512282 30 26.1 60.0 106.1779 35067 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 6.7 17.7 35.8 9.9 17.8 47.3 15.2 29.7 53.5 27.2 Excluded by Method 2.74 1 0.002079 0.009181 False High Sequest HT (A2) Unambiguous LFEDDDSNEK [K].lFEDDDsNEk.[L] N-Term(TMT6plex); S7(Phospho); K10(TMT6plex) 1 O43719 O43719 0 3 0.2621 0 1 1 583.93853 1749.80104 1749.79845 1.48 0.00087 493016.78125 CID (Collision Induced Dissociation) MS2 9.072847 60 68.8 60.0 89.1555 28686 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 66.2 68.5 52.8 78.9 65.4 71.0 78.9 72.2 65.0 68.9 Excluded by Method 2.9 1 0.002079 0.00958 False High Sequest HT (A2) Unambiguous RTCPSLSPTSPLNNK [K].rTcPSLsPTSPLNNk.[G] N-Term(TMT6plex); C3(Carbamidomethyl); S7(Phospho); K15(TMT6plex) 2 O75925 O75925; O75925-2 1 3 0.0258 0 1 1 737.38381 2210.13686 2210.14047 -1.63 -0.0012 694992.375 CID (Collision Induced Dissociation) MS2 5.195665 20 40.5 60.0 73.158 22673 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 27.4 41.6 41.2 37.1 40.2 54.2 42.3 42.0 48.1 30.5 Excluded by Method 3.1 1 0.002079 0.01265 False High Sequest HT (A2) Unambiguous DVTPPPETEVVLIK [K].dVtPPPETEVVLIk.[N] N-Term(TMT6plex); T3(Phospho); K14(TMT6plex) 3 P27816 P27816; P27816-6; P27816-2 0 3 0.2233 0 1 1 692.38662 2075.14532 2075.14415 0.56 0.00039 927691.625 CID (Collision Induced Dissociation) MS2 6.818353 20 148.5 60.0 116.4425 38793 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 161.2 141.2 113.0 155.0 139.8 147.6 171.8 163.1 140.5 152.2 Excluded by Method 3.18 1 0.002079 0.01112 False High Sequest HT (A2) Unambiguous ESEILERTPSPLK [K].eSEILERTPsPLk.[K] N-Term(TMT6plex); S10(Phospho); K13(TMT6plex) 1 P40157 P40157 1 4 0.0267 0 1 1 510.03127 2037.10325 2037.10334 -0.05 -2e-05 978975.9375 CID (Collision Induced Dissociation) MS2 0.0 70 58.3 60.0 84.0219 26702 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 14.4 46.1 78.6 20.7 47.2 106.7 22.1 60.4 122.2 65.1 2.62 1 0.002079 0.01265 False High Sequest HT (A2) Unambiguous LEETKESLQNK [K].lEETkEsLQNk.[G] N-Term(TMT6plex); K5(TMT6plex); S7(Phospho); K11(TMT6plex) 1 Q08245 Q08245 1 4 0.1344 0 1 1 522.29083 2086.1415 2086.14001 0.71 0.00037 1503744.125 CID (Collision Induced Dissociation) MS2 0.0 10 143.7 60.0 75.1925 23438 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 48.9 129.8 216.3 50.6 124.5 253.1 140.5 308.4 165.3 Excluded by Method 3.2 1 0.002079 0.01154 False High Sequest HT (A2) Unambiguous FTPVASKFSPGAPGGSGSQPNQK [K].fTPVAskFSPGAPGGSGSQPNQk.[L] N-Term(TMT6plex); S6(Phospho); K7(TMT6plex); K23(TMT6plex) 2 Q15942 Q15942; Q15942-2 1 4 0.0089 0 1 1 754.14993 3013.57789 3013.57515 0.91 0.00069 1940639.625 CID (Collision Induced Dissociation) MS2 1.450494 40 107.9 37.201 92.3143 29823 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 111.7 109.1 84.7 106.7 100.9 114.7 112.4 114.2 111.2 113.1 Excluded by Method 3.37 1 0.002079 0.01002 False High Sequest HT (A2) Unambiguous EEEEAKEESDDDMGFGLFD [K].eEEEAkEEsDDDmGFGLFD.[-] N-Term(TMT6plex); K6(TMT6plex); S9(Phospho); M13(Oxidation) 1 P05318 P05318 1 3 0.5429 0 1 1 916.05077 2746.13777 2746.13669 0.39 0.00036 1213366.125 CID (Collision Induced Dissociation) MS2 46.02059 50 93.2 58.88 119.2174 39866 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 28.6 79.0 135.0 31.9 77.2 165.7 37.3 92.0 194.4 90.7 Excluded by Method 3.15 1 0.002079 0.01071 False High Sequest HT (A2) Unambiguous DISGPSPSK [K].dISGPsPSk.[K] N-Term(TMT6plex); S6(Phospho); K9(TMT6plex) 2 Q96GX9 Q96GX9; Q96GX9-3 0 3 0.2993 0 1 1 475.91767 1425.73845 1425.73908 -0.45 -0.00021 4908603.5 CID (Collision Induced Dissociation) MS2 7.682343 60 274.5 43.003 57.5125 16768 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 287.1 266.1 209.4 309.6 283.0 256.6 312.7 292.7 254.3 273.0 Excluded by Method 3.04 1 0.002079 0.01136 False High Sequest HT (A2) Unambiguous EMEVEESPEK [K].eMEVEEsPEk.[I] N-Term(TMT6plex); S7(Phospho); K10(TMT6plex) 2 Q8N3X1 Q8N3X1-2; Q8N3X1 0 3 0.4208 0 1 1 582.27537 1744.81157 1744.81165 -0.05 -3e-05 3146645.75 CID (Collision Induced Dissociation) MS2 9.21457 60 45.8 34.94 66.6437 20149 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 44.5 49.6 40.4 42.7 44.5 46.0 52.3 44.0 49.6 44.4 Excluded by Method 2.21 1 0.002079 0.01185 False High Sequest HT (A2) Unambiguous AEFTSPPSLFK [K].aEFTsPPSLFk.[T] N-Term(TMT6plex); S5(Phospho); K11(TMT6plex) 4 Q6UN15 Q6UN15-4; Q6UN15-5; Q6UN15-3; Q6UN15 0 3 0.06 0 1 1 587.97895 1761.92229 1761.92286 -0.32 -0.00019 450139.78125 CID (Collision Induced Dissociation) MS2 9.953315 60 123.0 60.0 119.2551 39879 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 121.1 121.0 101.5 109.6 132.7 124.8 139.1 133.9 124.7 122.0 Excluded by Method 2.5 1 0.002079 0.01162 False High Sequest HT (A2) Unambiguous LEALSVK [K].lEALsVk.[E] N-Term(TMT6plex); S5(Phospho); K7(TMT6plex) 4 P43487 Q9C010-2; P43487; Q9C010; P43487-2 0 2 0.2007 0 1 1 649.38029 1297.75331 1297.75328 0.03 2e-05 6552965.5 CID (Collision Induced Dissociation) MS2 22.64673 20 162.2 24.865 92.7675 29991 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 157.3 152.1 127.8 164.3 154.7 168.7 175.0 177.4 171.2 173.2 Excluded by Method 2.74 1 0.002079 0.01297 False High Sequest HT (A2) Unambiguous EISTPTHWSK [K].eIStPTHWSk.[L] N-Term(TMT6plex); T4(Phospho); K10(TMT6plex) 2 Q8IX12 Q8IX12-2; Q8IX12 0 3 0.0396 0 1 1 575.29891 1723.88218 1723.88206 0.07 4e-05 679594.6875 CID (Collision Induced Dissociation) MS2 14.94651 40 73.2 60.0 77.1416 24124 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 73.3 73.5 62.5 59.8 74.0 83.0 78.1 80.0 67.2 81.1 Excluded by Method 2.02 1 0.002304 0.01428 False High Sequest HT (A2) Unambiguous TEVPGSPAGTEGNCQEATGPSTVDTQNEPLDMK [K].tEVPGsPAGTEGNcQEATGPSTVDTQNEPLDmk.[E] N-Term(TMT6plex); S6(Phospho); C14(Carbamidomethyl); M32(Oxidation); K33(TMT6plex) 3 Q32MZ4 Q32MZ4-2; Q32MZ4; Q32MZ4-3 0 4 0.1798 0 1 1 993.70338 3971.79169 3971.79279 -0.28 -0.00028 560188.5625 CID (Collision Induced Dissociation) MS2 44.56083 60 17.0 60.0 77.3635 24207 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 15.5 19.6 15.8 15.1 16.3 15.2 18.7 17.0 16.5 20.7 Excluded by Method 3.56 1 0.002304 0.0161 False High Sequest HT (A2) Unambiguous DRRGSNLQSHEQK [K].dRRGsNLQSHEQk.[F] N-Term(TMT6plex); S5(Phospho); K13(TMT6plex) 1 P16965 P16965 2 4 0.0792 0 1 1 524.02133 2093.06348 2093.06534 -0.88 -0.00046 63050.3671875 CID (Collision Induced Dissociation) MS2 41.39187 20 5.3 60.0 25.5626 5296 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 6.5 12.3 2.6 12.9 2.2 7.2 2.6 6.3 Excluded by Method 2.02 1 0.002304 0.01399 False High Sequest HT (A2) Unambiguous EEEAKEESDDDMGFGLFD [K].eEEAkEEsDDDmGFGLFD.[-] N-Term(TMT6plex); K5(TMT6plex); S8(Phospho); M12(Oxidation) 1 P02400 P02400 1 3 0.6106 0 1 1 873.03735 2617.09749 2617.0941 1.3 0.00113 1274443.75 CID (Collision Induced Dissociation) MS2 11.09359 20 133.3 56.547 121.7873 40859 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 28.4 110.8 198.0 43.8 106.6 251.9 50.8 130.5 270.5 141.5 Excluded by Method 3.21 1 0.002304 0.01526 False High Sequest HT (A2) Unambiguous STSNLASPMSSK [K].sTSNLAsPmSSk.[V] N-Term(TMT6plex); S7(Phospho); M9(Oxidation); K12(TMT6plex) 1 Q12271 Q12271 0 3 0.3469 0 1 1 588.62725 1763.86719 1763.86509 1.19 0.0007 407297.71875 CID (Collision Induced Dissociation) MS2 5.805849 60 56.5 60.0 52.5993 14972 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 19.5 49.4 95.7 21.1 47.2 105.7 21.3 51.4 103.4 50.7 Excluded by Method 3.2 1 0.002304 0.01627 False High Sequest HT (A2) Unambiguous TSELFPLSGSESGDIK [K].tSELFPLsGSESGDIk.[A] N-Term(TMT6plex); S8(Phospho); K16(TMT6plex) 1 P40563 P40563 0 3 0.0594 0 1 1 735.70812 2205.10981 2205.10922 0.27 0.0002 397275.96875 CID (Collision Induced Dissociation) MS2 8.374832 40 55.0 60.0 114.9888 38305 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 18.2 45.5 83.3 27.3 48.1 81.5 29.7 56.0 100.2 60.4 Excluded by Method 2.86 1 0.002304 0.01444 False High Sequest HT (A2) Unambiguous GGPGSAVSPYPTFNPSSDVAALHK [K].gGPGSAVsPYPTFNPSSDVAALHk.[A] N-Term(TMT6plex); S8(Phospho); K24(TMT6plex) 1 P04083 P04083 0 4 0.1263 0 1 1 724.366 2894.44216 2894.44899 -2.36 -0.00171 231745.1875 CID (Collision Induced Dissociation) MS2 46.88817 40 29.2 60.0 105.9078 34969 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 34.0 26.4 25.0 29.6 24.8 29.5 31.7 27.9 33.4 29.2 Excluded by Method 3.8 1 0.002304 0.01435 False High Sequest HT (A2) Unambiguous DSTNEFVGSPSLTSPQYIPSPLSSTKPPGSVNSAAK [K].dSTNEFVGSPSLTsPQYIPsPLSSTkPPGSVNSAAk.[Y] N-Term(TMT6plex); S14(Phospho); S20(Phospho); K26(TMT6plex); K36(TMT6plex) 1 P53114 P53114 0 4 0.0211 0 1 1 1124.56327 4495.23124 4495.22898 0.5 0.00056 339400.53125 CID (Collision Induced Dissociation) MS2 46.68563 60 17.0 60.0 120.4736 40377 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 3.9 20.5 26.7 7.2 15.3 27.1 10.6 13.4 29.3 15.9 Excluded by Method 3.32 1 0.002304 0.01404 False High Sequest HT (A2) Unambiguous KEESEESDDDMGFGLFD [K].kEESEEsDDDmGFGLFD.[-] N-Term(TMT6plex); K1(TMT6plex); S7(Phospho); M11(Oxidation) 3 P05387 P05386; P05386-2; P05387 1 2 0.0287 0 1 1 1252.52751 2504.04775 2504.04642 0.53 0.00067 1319591.875 CID (Collision Induced Dissociation) MS2 0.0 50 100.3 60.0 113.3139 37653 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 96.7 101.9 68.9 107.3 98.4 99.6 116.7 102.1 110.3 101.0 Excluded by Method 3.48 1 0.002304 0.01432 False High Sequest HT (A2) Unambiguous KDDSHSAEDSEDEK [K].kDDSHSAEDsEDEk.[E] N-Term(TMT6plex); K1(TMT6plex); S10(Phospho); K14(TMT6plex) 2 Q9H1E3 Q9H1E3; Q9H1E3-2 1 3 0.2877 0 1 1 787.03532 2359.0914 2359.09056 0.36 0.00028 420914.6875 CID (Collision Induced Dissociation) MS2 0.0 60 8.7 60.0 36.5273 9064 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 9.8 7.5 8.0 12.1 9.4 3.5 9.6 9.7 9.2 8.1 Excluded by Method 2.12 1 0.002304 0.01458 False High Sequest HT (A2) Unambiguous RGSVRGTSAADV [K].rGsVRGTSAADV.[-] N-Term(TMT6plex); S3(Phospho) 2 P48739 P48739; P48739-3 2 2 0.3374 0 1 1 742.87475 1484.74223 1484.74199 0.16 0.00012 669188.5625 CID (Collision Induced Dissociation) MS2 0.0 10 217.7 60.0 35.7977 8804 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 227.5 207.8 170.1 217.4 208.0 214.5 246.6 239.8 227.5 217.6 Excluded by Method 1.63 1 0.002304 0.0165 False High Sequest HT (A2) Unambiguous EFSIPRAHAWPSPYK [K].eFsIPRAHAWPSPYk.[D] N-Term(TMT6plex); S3(Phospho); K15(TMT6plex) 1 Q8NFJ5 Q8NFJ5 1 4 0.1462 0 1 1 581.80522 2324.19905 2324.19931 -0.11 -6e-05 1218479.125 CID (Collision Induced Dissociation) MS2 10.89859 50 31.7 60.0 101.3616 33189 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 36.8 28.6 24.8 30.9 31.7 32.7 35.1 35.6 28.3 32.3 Excluded by Method 2.12 1 0.002304 0.01551 False High Sequest HT (A2) Unambiguous NQSDAESIIPTPALNGYGK [K].nQsDAESIIPTPALNGYGk.[G] N-Term(TMT6plex); S3(Phospho); K19(TMT6plex) 1 P27637 P27637 0 3 0.0714 0 1 1 838.42612 2513.26381 2513.26891 -2.03 -0.0017 793002.875 CID (Collision Induced Dissociation) MS2 25.2269 60 35.8 60.0 113.9072 37908 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 8.1 36.3 53.6 6.9 31.3 66.9 14.6 35.6 67.1 37.7 Excluded by Method 3.36 1 0.002304 0.01575 False High Sequest HT (A2) Unambiguous HIVSNDSSDSDDESHEPK [K].hIVSNDSSDsDDESHEPk.[G] N-Term(TMT6plex); S10(Phospho); K18(TMT6plex) 1 P47712 P47712 0 4 0.1136 0 1 1 634.78675 2536.12516 2536.12409 0.42 0.00027 265328.75 CID (Collision Induced Dissociation) MS2 5.04053 10 26.8 60.0 33.8036 8149 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 25.2 24.5 22.0 35.3 28.1 22.0 35.2 31.6 12.6 31.4 Excluded by Method 2.64 1 0.002304 0.01616 False High Sequest HT (A2) Unambiguous LQALPTP [K].lQALPtP.[-] N-Term(TMT6plex); T6(Phospho) 1 Q06188 Q06188 0 2 0.6888 0 1 1 524.78561 1048.56394 1048.56413 -0.18 -9e-05 457558.875 CID (Collision Induced Dissociation) MS2 0.0 50 93.0 60.0 101.2052 33132 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 23.6 77.9 150.9 28.3 71.2 180.2 38.4 85.5 185.2 88.6 Excluded by Method 1.96 1 0.002304 0.01317 False High Sequest HT (A2) Unambiguous IAISPLK [K].iAIsPLk.[S] N-Term(TMT6plex); S4(Phospho); K7(TMT6plex) 2 Q6MZP7 Q6MZP7-3; Q6MZP7 0 2 0.0982 0 1 1 640.39343 1279.77959 1279.7791 0.38 0.00024 1121745.375 CID (Collision Induced Dissociation) MS2 17.00512 20 68.4 60.0 100.9469 33033 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 72.1 70.4 53.0 75.8 69.0 75.9 76.6 68.9 62.6 59.2 Excluded by Method 2.85 1 0.002304 0.01339 False High Sequest HT (A2) Unambiguous NDDSHDDLILLSAK [K].nDDsHDDLILLSAk.[K] N-Term(TMT6plex); S4(Phospho); K14(TMT6plex) 1 Q06673 Q06673 0 3 0.0641 0 1 1 698.68886 2094.05202 2094.05204 -0.01 -1e-05 1148273.25 CID (Collision Induced Dissociation) MS2 4.939526 60 38.7 60.0 102.9453 33775 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 21.0 34.0 48.5 25.7 37.6 51.6 30.4 39.2 60.7 38.2 Excluded by Method 1.56 1 0.002304 0.01417 False High Sequest HT (A2) Unambiguous ARPFYLTHLITQSVSPK [K].aRPFYLTHLITQSVsPk.[N] N-Term(TMT6plex); S15(Phospho); K17(TMT6plex) 1 Q12163 Q12163 0 4 0.082 0 1 1 624.85078 2496.3813 2496.378 1.32 0.00082 288793.78125 CID (Collision Induced Dissociation) MS2 0.0 50 30.5 60.0 107.1647 35429 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 12.5 27.0 46.5 15.6 28.2 57.2 11.1 28.1 50.6 28.0 Excluded by Method 2.56 1 0.002304 0.01371 False High Sequest HT (A2) Unambiguous ELESPLTPGK [K].eLESPLtPGk.[V] N-Term(TMT6plex); T7(Phospho); K10(TMT6plex) 1 O15014 O15014 0 3 0.3904 0 1 1 536.96004 1608.86555 1608.86501 0.34 0.00018 2471570.75 CID (Collision Induced Dissociation) MS2 20.45663 40 21.9 51.87 83.853 26641 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 15.0 17.5 19.6 18.9 21.8 27.0 17.1 29.2 34.3 18.5 Excluded by Method 2.28 1 0.002304 0.01459 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVssDEEF.[-] N-Term(TMT6plex); S8(Phospho); S9(Phospho) 1 P16521 P16521 0 2 0.239 0 1 1 925.35565 1849.70403 1849.70195 1.12 0.00104 575776.0625 CID (Collision Induced Dissociation) MS2 11.32338 40 45.9 60.0 138.1168 47114 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 12.0 43.0 75.5 16.3 34.7 89.6 20.0 39.6 88.0 40.8 Excluded by Method 2.05 1 0.002304 0.01388 False High Sequest HT (A2) Unambiguous APLKPYPVSPSDK [K].aPLkPYPVsPSDk.[V] N-Term(TMT6plex); K4(TMT6plex); S9(Phospho); K13(TMT6plex) 1 P41252 P41252 0 3 0.0665 0 1 1 722.74436 2166.21851 2166.21787 0.3 0.00021 4344490.5 CID (Collision Induced Dissociation) MS2 22.73023 40 95.1 19.041 83.7461 26663 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 86.4 79.5 89.2 78.9 93.9 111.0 93.5 99.1 109.0 110.2 Excluded by Method 3.76 1 0.002304 0.01763 False High Sequest HT (A2) Unambiguous LEETKESLQNK [K].lEETkEsLQNk.[G] N-Term(TMT6plex); K5(TMT6plex); S7(Phospho); K11(TMT6plex) 1 Q08245 Q08245 1 3 0.225 0 1 1 696.05235 2086.1425 2086.14001 1.19 0.00083 656122.125 CID (Collision Induced Dissociation) MS2 0.0 0 195.6 60.0 74.4947 23115 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 41.9 151.4 269.0 65.2 168.8 362.4 80.3 196.4 405.6 215.2 Excluded by Method 3.6 1 0.002304 0.01631 False High Sequest HT (A2) Unambiguous EEEAAEESDDDMGFGLFD [K].eEEAAEEsDDDmGFGLFD.[-] N-Term(TMT6plex); S8(Phospho); M12(Oxidation) 2 P05319 P10622; P05319 0 2 0.7 0 1 1 1165.94298 2330.87869 2330.87332 2.31 0.00269 315234.34375 CID (Collision Induced Dissociation) MS2 51.02992 70 28.2 60.0 148.8967 50825 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 7.1 20.2 49.1 9.7 24.2 53.4 8.5 24.0 57.8 27.8 2.4 1 0.002304 0.01354 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVsSDEEF.[-] N-Term(TMT6plex); S8(Phospho) 1 P16521 P16521 0 2 0.0783 0 1 1 885.37241 1769.73754 1769.73562 1.08 0.00096 391166.84375 CID (Collision Induced Dissociation) MS2 21.04215 50 32.5 60.0 134.2163 45752 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 9.7 27.6 49.6 12.6 31.3 57.9 11.6 24.4 64.8 35.9 Excluded by Method 2.3 1 0.002304 0.01464 False High Sequest HT (A2) Unambiguous LVADGVFYAELNEFFTRELAEEGYSGVEVRVTPTK [K].lVADGVFYAELNEFFTRELAEEGYSGVEVRVTPTk.[T] N-Term(TMT6plex); K35(TMT6plex) 1 P05750 P05750 2 5 0.3475 0 1 1 879.6685 4394.31339 4394.29604 3.95 0.00347 667728.0 CID (Collision Induced Dissociation) MS2 0.0 60 18.4 60.0 132.0624 44953 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 11.7 15.6 17.5 12.8 17.7 31.8 11.1 21.1 28.3 16.4 Excluded by Method 3.54 1 0.002304 0.0158 False High Sequest HT (A2) Unambiguous NLLSSSDSRRNSQDEDSLPNNTNLIK [K].nLLSSSDSRRNsQDEDsLPNNTNLIk.[E] N-Term(TMT6plex); S12(Phospho); S17(Phospho); K26(TMT6plex) 1 Q08280 Q08280 2 4 0.0056 0 1 1 884.67747 3535.68806 3535.68312 1.4 0.00124 1437348.0 CID (Collision Induced Dissociation) MS2 0.0 50 7.0 60.0 98.8238 32243 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 2.7 3.6 7.3 6.7 8.1 11.1 7.6 8.7 7.5 6.2 Excluded by Method 3.59 1 0.002304 0.01505 False High Sequest HT (A2) Unambiguous QLELSINSASPNVNHSPVPTK [K].qLELSINSASPNVNHsPVPTk.[K] N-Term(TMT6plex); S16(Phospho); K21(TMT6plex) 1 P32457 P32457 0 4 0.072 0 1 1 693.36862 2770.45264 2770.45408 -0.52 -0.00036 1636743.625 CID (Collision Induced Dissociation) MS2 11.22056 60 62.1 60.0 94.2804 30526 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 41.3 59.8 70.1 47.8 51.1 89.7 60.3 61.8 72.3 66.5 Excluded by Method 2.5 1 0.002304 0.01435 False High Sequest HT (A2) Unambiguous FQYIAISQSDADSESCK [K].fQYIAISQSDADSESck.[M] N-Term(TMT6plex); C16(Carbamidomethyl); K17(TMT6plex) 1 P38013 P38013 0 3 0.4068 0 1 1 803.06648 2407.18488 2407.18516 -0.12 -0.0001 2093868.25 CID (Collision Induced Dissociation) MS2 43.87744 40 13.4 60.0 98.5865 32154 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 9.7 10.1 14.4 7.3 11.3 20.7 8.5 14.8 24.5 12.8 Excluded by Method 2.36 1 0.002304 0.01782 False High Sequest HT (A2) Unambiguous WLISPVK [K].wLIsPVk.[I] N-Term(TMT6plex); S4(Phospho); K7(TMT6plex) 3 Q8NCD3 Q8NCD3-2; Q8NCD3-3; Q8NCD3 0 3 0.4021 0 1 1 460.93994 1380.80528 1380.80565 -0.27 -0.00012 1003951.3125 CID (Collision Induced Dissociation) MS2 24.9673 40 137.9 60.0 119.2715 39935 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 146.6 136.6 112.9 141.6 136.6 136.0 156.6 149.9 127.8 134.5 Excluded by Method 2.86 1 0.002304 0.01603 False High Sequest HT (A2) Unambiguous DDGSDRDSDREQDEK [K].dDGsDRDsDREQDEk.[Q] N-Term(TMT6plex); S4(Phospho); S8(Phospho); K15(TMT6plex) 1 Q9UGP8 Q9UGP8 2 3 0.3893 0 1 1 795.66013 2384.96583 2384.96449 0.56 0.00045 118565.8046875 CID (Collision Induced Dissociation) MS2 21.78759 40 27.5 60.0 29.6344 6686 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 21.4 28.6 19.8 28.6 28.0 25.8 34.3 37.9 27.0 23.8 Excluded by Method 2.44 1 0.002304 0.01753 False High Sequest HT (A2) Unambiguous AEFTSPPSLFK [K].aEFTsPPSLFk.[T] N-Term(TMT6plex); S5(Phospho); K11(TMT6plex) 4 Q6UN15 Q6UN15-4; Q6UN15-5; Q6UN15-3; Q6UN15 0 3 0.0672 0 1 1 587.9793 1761.92335 1761.92286 0.28 0.00016 1257443.625 CID (Collision Induced Dissociation) MS2 3.53137 70 106.9 60.0 120.8054 40496 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 104.6 109.3 95.5 105.7 105.6 102.3 121.2 104.8 112.2 107.5 2.53 1 0.002304 0.01568 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVSsDEEF.[-] N-Term(TMT6plex); S9(Phospho) 1 P16521 P16521 0 2 0.02 0 1 1 885.37278 1769.73829 1769.73562 1.51 0.00133 166426.9375 CID (Collision Induced Dissociation) MS2 35.69462 60 33.6 60.0 145.239 49562 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 11.5 28.0 53.6 8.7 26.5 60.7 12.1 35.9 66.2 32.9 Excluded by Method 2.5 1 0.002304 0.01432 False High Sequest HT (A2) Unambiguous LFEESDDK [K].lFEEsDDk.[E] N-Term(TMT6plex); S5(Phospho); K8(TMT6plex) 1 O43719 O43719 0 3 0.3125 0 1 1 507.58138 1520.72959 1520.72858 0.67 0.00034 1520082.25 CID (Collision Induced Dissociation) MS2 2.767731 40 32.2 60.0 87.1621 27883 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 30.3 32.3 29.6 29.8 28.2 40.8 29.5 33.7 37.8 29.7 Excluded by Method 2.4 1 0.002304 0.01433 False High Sequest HT (A2) Unambiguous IAALAGQQ [K].iAALAGQQ.[-] N-Term(TMT6plex) 1 P26321 P26321 0 2 0.6784 0 1 1 500.80335 1000.59941 1000.59886 0.55 0.00028 2001184.125 CID (Collision Induced Dissociation) MS2 5.424277 50 64.0 60.0 75.4692 23488 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 33.9 56.3 70.0 40.3 61.1 101.9 42.5 62.2 104.0 67.6 Excluded by Method 1.71 1 0.002304 0.0151 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVSsDEEF.[-] N-Term(TMT6plex); S9(Phospho) 1 P16521 P16521 0 2 0.0428 0 1 1 885.37253 1769.73779 1769.73562 1.22 0.00108 286958.28125 CID (Collision Induced Dissociation) MS2 0.0 50 32.0 60.0 143.1513 48848 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 6.8 28.0 42.7 12.7 23.3 65.6 14.8 24.7 64.3 37.4 Excluded by Method 2.57 1 0.002304 0.01447 False High Sequest HT (A2) Unambiguous SANEFSTTPEDK [K].sANEFsTTPEDk.[H] N-Term(TMT6plex); S1(Phospho); S6(Phospho); K12(TMT6plex) 1 Q06315 Q06315 0 3 0.0319 0 1 1 648.61961 1943.84429 1943.84409 0.1 7e-05 1594456.375 CID (Collision Induced Dissociation) MS2 3.437337 50 78.5 48.342 71.4859 21985 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 27.1 65.8 117.7 28.6 66.0 140.2 29.7 74.7 161.1 73.6 Excluded by Method 2.82 1 0.002304 0.01603 False High Sequest HT (A2) Unambiguous DMSPLSETEMALGK [K].dMsPLSETEmALGk.[D] N-Term(TMT6plex); S3(Phospho); M10(Oxidation); K14(TMT6plex) 3 P27816 P27816; P27816-6; P27816-2 0 3 0.0766 0 1 1 688.33347 2062.98585 2062.98422 0.79 0.00055 545623.875 CID (Collision Induced Dissociation) MS2 52.80325 60 46.4 60.0 122.1014 40892 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 39.7 42.2 43.2 30.4 42.8 60.2 34.4 56.9 61.5 53.0 Excluded by Method 2.35 1 0.002304 0.01415 False High Sequest HT (A2) Unambiguous RIVAPPGGRSNITSLS [K].rIVAPPGGRSNITSLs.[-] N-Term(TMT6plex); S16(Phospho) 2 Q14195-2 Q14195-2; Q14195 2 3 0.2679 0 1 1 645.35235 1934.04248 1934.04219 0.15 0.0001 871509.375 CID (Collision Induced Dissociation) MS2 21.60379 60 50.2 60.0 71.6345 22042 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 36.9 88.7 42.4 53.1 45.2 49.7 41.5 48.4 45.5 50.1 Excluded by Method 2.8 1 0.002304 0.01737 False High Sequest HT (A2) Unambiguous IESPESIK [K].iEsPEsIk.[E] N-Term(TMT6plex); S3(Phospho); S6(Phospho); K8(TMT6plex) 1 P23201 P23201 0 2 0.316 0 1 1 760.8749 1520.74251 1520.74146 0.69 0.00053 446497.28125 CID (Collision Induced Dissociation) MS2 13.89851 10 97.9 60.0 83.8802 26651 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 28.8 88.8 139.1 39.8 78.8 172.5 42.6 96.4 193.7 98.5 Excluded by Method 2.12 1 0.002304 0.01595 False High Sequest HT (A2) Unambiguous QSFDDNDSEELEDK [K].qSFDDNDsEELEDk.[D] N-Term(TMT6plex); S8(Phospho); K14(TMT6plex) 1 O60841 O60841 0 3 0.0468 0 1 1 736.98995 2208.95528 2208.95859 -1.5 -0.0011 345259.90625 CID (Collision Induced Dissociation) MS2 24.44756 60 41.1 60.0 81.5093 25816 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 40.5 46.1 35.8 38.8 35.8 38.7 47.7 43.9 48.4 35.1 Excluded by Method 2.78 1 0.002304 0.0141 False High Sequest HT (A2) Unambiguous VQGEAVSNIQENTQTPTVQEESEEEEVDETGVEVK [K].vQGEAVSNIQENTQTPTVQEESEEEEVDEtGVEVk.[D] N-Term(TMT6plex); T30(Phospho); K35(TMT6plex) 3 Q13765 Q13765; E9PAV3; E9PAV3-2 0 4 0.0091 0 1 1 1100.52086 4399.06163 4399.06016 0.33 0.00037 184448.859375 CID (Collision Induced Dissociation) MS2 20.46829 30 6.8 60.0 141.3379 48219 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 9.0 7.4 8.8 4.1 6.5 8.3 8.5 8.0 3.7 4.1 Excluded by Method 3.31 1 0.002304 0.01675 False High Sequest HT (A2) Unambiguous LESESTSPSLEMK [K].lESESTsPSLEmk.[I] N-Term(TMT6plex); S7(Phospho); M12(Oxidation); K13(TMT6plex) 4 Q5VT52 Q5VT52-5; Q5VT52; Q5VT52-2; Q5VT52-3 0 3 0.0552 0 1 1 664.66012 1991.96581 1991.96486 0.47 0.00032 1466173.125 CID (Collision Induced Dissociation) MS2 48.75159 40 30.9 39.305 75.3057 23421 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 31.2 27.1 20.8 36.8 27.6 31.4 29.7 37.8 37.1 30.0 Excluded by Method 2.9 1 0.002304 0.01512 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVssDEEF.[-] N-Term(TMT6plex); S8(Phospho); S9(Phospho) 1 P16521 P16521 0 2 0.2076 0 1 1 925.35502 1849.70277 1849.70195 0.44 0.00041 672396.5625 CID (Collision Induced Dissociation) MS2 7.253429 50 59.2 60.0 143.5278 48979 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 15.3 54.9 92.1 20.5 46.7 113.3 20.1 53.0 123.9 51.9 Excluded by Method 2.36 1 0.002304 0.01387 False High Sequest HT (A2) Unambiguous VMIGSPK [K].vmIGsPk.[K] N-Term(TMT6plex); M2(Oxidation); S5(Phospho); K7(TMT6plex) 2 Q68CZ2 Q68CZ2; Q68CZ2-2 0 3 0.3711 0 1 1 429.23771 1285.69857 1285.69913 -0.44 -0.00019 324932.25 CID (Collision Induced Dissociation) MS2 49.78657 30 53.5 60.0 67.8183 20658 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 44.2 55.5 38.0 65.8 50.5 49.4 61.5 62.2 57.1 51.2 Excluded by Method 3.18 1 0.002304 0.01394 False High Sequest HT (A2) Unambiguous RSASVGSNQSEQDK [K].rSAsVGSNQSEQDk.[G] N-Term(TMT6plex); S4(Phospho); K14(TMT6plex) 1 P08018 P08018 1 3 0.1018 0 1 1 677.66884 2030.99197 2030.99083 0.56 0.00038 144257.453125 CID (Collision Induced Dissociation) MS2 35.69112 70 8.0 60.0 32.8718 7813 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 7.3 11.7 2.1 8.1 14.3 2.7 2.7 21.6 9.3 2.26 1 0.002304 0.01578 False High Sequest HT (A2) Unambiguous QASASYDSEEEEEGLPMSYDEK [K].qASAsYDSEEEEEGLPmSYDEk.[R] N-Term(TMT6plex); S5(Phospho); M17(Oxidation); K22(TMT6plex) 1 Q15059 Q15059 0 3 0.0237 0 1 1 1016.77037 3048.29657 3048.29571 0.28 0.00029 758514.5 CID (Collision Induced Dissociation) MS2 0.0 50 10.7 60.0 83.3694 26433 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 7.8 12.1 7.1 9.1 12.0 10.1 11.6 12.5 13.9 10.8 Excluded by Method 2.53 1 0.002304 0.01761 False High Sequest HT (A2) Unambiguous TSPENVQDRFALVTPK [K].tSPENVQDRFALVtPk.[K] N-Term(TMT6plex); T1(Phospho); T14(Phospho); K16(TMT6plex) 1 P49792 P49792 1 3 0.436 0 1 1 807.40546 2420.20183 2420.20281 -0.41 -0.00033 723408.875 CID (Collision Induced Dissociation) MS2 0.0 40 104.0 60.0 98.267 32121 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 92.4 100.8 95.6 110.7 91.1 102.4 119.0 118.7 109.0 100.5 Excluded by Method 2.89 1 0.002304 0.01597 False High Sequest HT (A2) Unambiguous ISSSEDFSGK [K].iSsSEDFSGk.[D] N-Term(TMT6plex); S3(Phospho); K10(TMT6plex) 1 P38065 P38065 0 2 0.0936 0 1 1 797.89241 1594.77754 1594.77659 0.6 0.00047 659947.25 CID (Collision Induced Dissociation) MS2 0.0 10 72.9 60.0 76.3402 23872 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 18.4 63.1 99.7 24.6 68.9 129.7 24.6 67.6 154.4 77.6 Excluded by Method 2.35 1 0.002304 0.01385 False High Sequest HT (A2) Unambiguous TQSASPPPK [K].tQSAsPPPk.[E] N-Term(TMT6plex); S5(Phospho); K9(TMT6plex) 1 Q9UN81 Q9UN81 0 3 0.0789 0 1 1 484.26196 1450.77131 1450.77072 0.41 0.0002 255885.921875 CID (Collision Induced Dissociation) MS2 20.99196 60 23.6 60.0 43.4107 11598 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 17.2 25.6 19.5 25.6 22.2 25.6 27.4 24.3 25.0 23.5 Excluded by Method 1.9 1 0.002304 0.01418 False High Sequest HT (A2) Unambiguous DISGPSPSK [K].dISGPsPSk.[K] N-Term(TMT6plex); S6(Phospho); K9(TMT6plex) 2 Q96GX9 Q96GX9; Q96GX9-3 0 3 0.3288 0 1 1 475.91784 1425.73896 1425.73908 -0.08 -4e-05 944210.625 CID (Collision Induced Dissociation) MS2 5.928361 70 277.9 60.0 58.3712 17060 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 286.8 267.6 216.6 296.2 265.8 271.6 335.4 294.8 265.9 278.4 2.95 1 0.002304 0.01606 False High Sequest HT (A2) Unambiguous NKPGPNIESGNEDDDASFK [K].nkPGPNIEsGNEDDDASFk.[I] N-Term(TMT6plex); K2(TMT6plex); S9(Phospho); K19(TMT6plex) 1 O60841 O60841 0 5 0.3322 0 1 1 561.07729 2801.35734 2801.35979 -0.88 -0.00049 719976.125 CID (Collision Induced Dissociation) MS2 17.9832 20 45.0 60.0 76.2278 23828 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 46.5 39.5 34.6 47.7 42.2 50.6 54.3 45.8 45.5 43.6 Excluded by Method 3.04 1 0.002304 0.01703 False High Sequest HT (A2) Unambiguous TVAELMEN [K].tVAELmEN.[-] N-Term(TMT6plex); M6(Oxidation) 1 P53981 P53981 0 2 0.3316 0 1 1 576.29464 1151.582 1151.58156 0.38 0.00022 1165856.0 CID (Collision Induced Dissociation) MS2 5.567182 70 157.8 60.0 60.9004 18034 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 43.3 124.8 271.4 43.9 122.7 293.3 54.7 145.2 317.5 161.0 1.9 1 0.002304 0.01409 False High Sequest HT (A2) Unambiguous IAISPLK [K].iAIsPLk.[S] N-Term(TMT6plex); S4(Phospho); K7(TMT6plex) 2 Q6MZP7 Q6MZP7-3; Q6MZP7 0 2 0.086 0 1 1 640.3938 1279.78031 1279.7791 0.95 0.00061 526187.25 CID (Collision Induced Dissociation) MS2 15.08861 30 82.6 60.0 99.9663 32694 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 81.9 86.1 72.4 84.6 74.7 83.1 97.6 93.2 76.1 76.3 Excluded by Method 2.79 1 0.002304 0.0134 False High Sequest HT (A2) Unambiguous SSLETIVEK [K].sSLETIVEk.[K] N-Term(TMT6plex); S1(Phospho); K9(TMT6plex) 1 P47035 P47035 0 3 0.025 0 1 1 515.28477 1543.83976 1543.83846 0.84 0.00043 483511.5625 CID (Collision Induced Dissociation) MS2 44.02625 50 35.6 60.0 96.2701 31310 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 16.2 27.7 41.7 22.3 31.7 60.0 26.0 40.8 56.9 33.0 Excluded by Method 2.4 1 0.002304 0.01423 False High Sequest HT (A2) Unambiguous YNDVIEDESDDDNMSTDEAK [K].yNDVIEDEsDDDNmSTDEAk.[N] N-Term(TMT6plex); S9(Phospho); M14(Oxidation); K20(TMT6plex) 1 P48415 P48415 0 3 0.0868 0 1 1 953.73038 2859.17658 2859.18035 -1.32 -0.00125 340081.3125 CID (Collision Induced Dissociation) MS2 14.2573 50 21.3 60.0 77.7923 24419 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 10.2 22.6 25.9 11.9 18.1 39.2 10.6 21.6 34.2 18.2 Excluded by Method 2.19 1 0.002304 0.01506 False High Sequest HT (A2) Unambiguous YRIQEQESSGEEDSDLSPEEREK [K].yRIQEQEssGEEDSDLSPEEREk.[K] N-Term(TMT6plex); S8(Phospho); S9(Phospho); K23(TMT6plex) 2 Q6NXS1 Q6NXS1; P41236 2 3 0.3261 0 1 1 1120.16118 3358.46898 3358.47654 -2.25 -0.00252 1015442.375 CID (Collision Induced Dissociation) MS2 42.0285 20 54.6 60.0 70.8551 21724 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 60.1 56.9 47.6 49.2 44.1 53.0 65.8 59.7 57.5 51.8 Excluded by Method 3.22 1 0.002304 0.01375 False High Sequest HT (A2) Unambiguous LFPGSPAIYK [K].lFPGsPAIYk.[L] N-Term(TMT6plex); S5(Phospho); K10(TMT6plex) 9 P49792 P0DJD0; P49792; Q99666; Q7Z3J3-2; P0DJD1; Q99666-2; O14715; A6NKT7; Q7Z3J3 0 2 0.2932 0 1 1 815.95531 1630.90334 1630.901 1.43 0.00117 1765691.5 CID (Collision Induced Dissociation) MS2 0.0 30 55.9 60.0 118.0635 39396 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 53.5 65.2 47.1 47.8 49.1 65.1 49.4 65.3 67.3 49.6 Excluded by Method 3.65 1 0.002304 0.01459 False High Sequest HT (A2) Unambiguous CSGPGLSPGMVRANLPQSFQVDTSK [K].cSGPGLsPGMVRANLPQSFQVDTSk.[A] N-Term(TMT6plex); C1(Carbamidomethyl); S7(Phospho); K25(TMT6plex) 2 P21333 P21333; P21333-2 1 3 0.1343 0 1 1 1057.86058 3171.56718 3171.57323 -1.91 -0.00201 595162.4375 CID (Collision Induced Dissociation) MS2 4.211496 30 41.5 60.0 116.8708 39035 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 34.1 46.9 33.4 44.7 38.9 38.3 42.8 43.5 55.5 36.9 Excluded by Method 2.83 1 0.002304 0.01403 False High Sequest HT (A2) Unambiguous TSVSQNVIPSSAQK [K].tSVSQNVIPSsAQk.[R] N-Term(TMT6plex); S11(Phospho); K14(TMT6plex) 2 Q03188 Q03188; Q03188-2 0 3 0.0795 0 1 1 662.02231 1984.05238 1984.05164 0.37 0.00025 2091819.0 CID (Collision Induced Dissociation) MS2 2.064486 60 40.2 49.116 73.8896 22953 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 40.1 36.5 29.6 44.6 33.5 40.8 50.6 41.3 42.8 42.4 Excluded by Method 2.39 1 0.002304 0.01611 False High Sequest HT (A2) Unambiguous SSLESPTMLK [K].sSLEsPTmLk.[L] N-Term(TMT6plex); S5(Phospho); M8(Oxidation); K10(TMT6plex) 1 Q12044 Q12044 0 3 0.096 0 1 1 549.6212 1646.84904 1646.84765 0.84 0.00046 1202719.75 CID (Collision Induced Dissociation) MS2 7.145415 20 140.9 60.0 77.5572 24246 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 30.4 115.2 211.3 44.1 101.8 275.1 48.4 132.4 282.2 167.7 Excluded by Method 3.23 1 0.002304 0.01617 False High Sequest HT (A2) Unambiguous QAREESEESEAEPVQRTAK [K].qAREEsEEsEAEPVQRTAk.[K] N-Term(TMT6plex); S6(Phospho); S9(Phospho); K19(TMT6plex) 1 Q9BW71 Q9BW71 2 3 0.384 0 1 1 931.43521 2792.29109 2792.29052 0.2 0.00019 1696338.5 CID (Collision Induced Dissociation) MS2 0.0 30 156.3 60.0 46.6402 12766 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 162.4 159.8 128.7 159.2 156.6 153.3 161.2 173.5 157.3 150.9 Excluded by Method 2.5 1 0.002304 0.01481 False High Sequest HT (A2) Unambiguous ASGRGAESPFEEK [K].aSGRGAEsPFEEk.[S] N-Term(TMT6plex); S8(Phospho); K13(TMT6plex) 1 P46821 P46821 1 3 0.3795 0 1 1 634.98346 1902.93584 1902.93628 -0.23 -0.00015 41245316.0 CID (Collision Induced Dissociation) MS2 4.035169 30 148.5 8.329 55.7287 16111 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 154.4 151.4 122.9 148.7 149.5 153.8 158.9 148.5 148.2 149.1 Excluded by Method 3.03 1 0.002304 0.01333 False High Sequest HT (A2) Unambiguous VEPVEGSLQSK [K].vEPVEGsLQSk.[L] N-Term(TMT6plex); S7(Phospho); K11(TMT6plex) 1 P38845 P38845 0 3 0.2006 0 1 1 570.97401 1710.90747 1710.90794 -0.27 -0.00016 538408.25 CID (Collision Induced Dissociation) MS2 0.0 40 67.5 60.0 74.7841 23285 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 18.7 52.6 83.5 34.1 63.0 121.0 31.4 67.0 134.2 69.4 Excluded by Method 3.49 1 0.002304 0.01549 False High Sequest HT (A2) Unambiguous KQSFDDNDSEELEDK [K].kQsFDDNDsEELEDk.[D] N-Term(TMT6plex); K1(TMT6plex); S3(Phospho); S9(Phospho); K15(TMT6plex) 1 O60841 O60841 1 3 0.4216 0 1 1 882.73399 2646.18743 2646.18282 1.74 0.00154 574895.25 CID (Collision Induced Dissociation) MS2 0.0 10 73.7 60.0 78.4697 24673 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 73.5 60.4 54.9 98.3 68.2 70.3 88.7 77.2 72.4 73.1 Excluded by Method 3.7 1 0.002304 0.01381 False High Sequest HT (A2) Unambiguous WFFISGSV [K].wFFISGsV.[-] N-Term(TMT6plex); S7(Phospho) 1 P00817 P00817 0 2 0.1718 0 1 1 626.30477 1251.60227 1251.60124 0.82 0.00051 207795.375 CID (Collision Induced Dissociation) MS2 0.0 40 26.0 60.0 140.368 47919 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 3.8 19.0 42.0 9.8 15.9 57.2 10.0 26.5 50.8 25.0 Excluded by Method 2.27 1 0.002304 0.01329 False High Sequest HT (A2) Unambiguous EEEEAKEESDDDMGFGLFD [K].eEEEAkEEsDDDmGFGLFD.[-] N-Term(TMT6plex); K6(TMT6plex); S9(Phospho); M13(Oxidation) 1 P05318 P05318 1 3 0.6635 0 1 1 916.05059 2746.1372 2746.13669 0.19 0.00017 1741460.875 CID (Collision Induced Dissociation) MS2 13.03806 30 138.4 35.386 116.8424 38941 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 31.5 115.2 204.3 47.2 126.2 245.4 49.2 124.7 301.8 138.3 Excluded by Method 3.15 1 0.002304 0.01656 False High Sequest HT (A2) Unambiguous TVAELMEN [K].tVAELmEN.[-] N-Term(TMT6plex); M6(Oxidation) 1 P53981 P53981 0 2 0.3981 0 1 1 576.29498 1151.58268 1151.58156 0.97 0.00056 774522.375 CID (Collision Induced Dissociation) MS2 8.385663 70 103.9 60.0 61.7829 18338 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 25.7 81.1 157.7 38.6 84.7 207.5 42.0 96.2 202.9 103.1 2.16 1 0.002304 0.01493 False High Sequest HT (A2) Unambiguous DGNSSPQEFK [K].dGNsSPQEFk.[L] N-Term(TMT6plex); S4(Phospho); K10(TMT6plex) 1 Q07084 Q07084 0 3 0.0113 0 1 1 549.59936 1646.78353 1646.78274 0.48 0.00026 286440.15625 CID (Collision Induced Dissociation) MS2 16.0948 50 46.3 60.0 59.158 17377 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 33.6 44.4 39.0 41.0 42.2 61.0 46.4 45.2 67.6 42.8 Excluded by Method 1.77 1 0.002304 0.01448 False High Sequest HT (A2) Unambiguous SISPEVSASRRK [K].sISPEVSASRRk.[L] N-Term(TMT6plex); S1(Phospho); K12(TMT6plex) 1 Q9Y2F5 Q9Y2F5 2 4 0.0365 0 1 1 464.5106 1855.02058 1855.02028 0.16 7e-05 410795.28125 CID (Collision Induced Dissociation) MS2 3.229911 20 28.5 60.0 50.843 14268 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 33.4 25.1 25.2 27.1 22.9 33.7 29.3 31.8 25.1 31.8 Excluded by Method 3.01 1 0.002304 0.01726 False High Sequest HT (A2) Unambiguous TQSASPPPK [K].tQSAsPPPk.[E] N-Term(TMT6plex); S5(Phospho); K9(TMT6plex) 1 Q9UN81 Q9UN81 0 3 0.0971 0 1 1 484.2616 1450.77026 1450.77072 -0.32 -0.00015 103559.1015625 CID (Collision Induced Dissociation) MS2 17.63332 10 69.4 60.0 43.4722 11573 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 22.0 53.3 112.2 25.6 64.4 123.8 28.9 66.3 135.3 62.4 Excluded by Method 2.06 1 0.002304 0.01717 False High Sequest HT (A2) Unambiguous SREPTGVEENK [K].sREPTGVEENk.[T] N-Term(TMT6plex); S1(Phospho); K11(TMT6plex) 2 Q7Z6E9 Q7Z6E9-2; Q7Z6E9 1 3 0.2908 0 1 1 595.30483 1783.89994 1783.89916 0.43 0.00026 301529.25 CID (Collision Induced Dissociation) MS2 0.0 20 26.2 60.0 43.5208 11590 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 25.1 31.8 15.4 25.5 28.3 24.2 27.6 24.5 34.2 25.0 Excluded by Method 2.82 1 0.002304 0.01635 False High Sequest HT (A2) Unambiguous DSSVPLIIDSSGSAN [K].dSsVPLIIDSSGSAN.[-] N-Term(TMT6plex); S3(Phospho) 1 P38310 P38310 0 2 0.0049 0 1 1 885.9227 1770.83813 1770.83601 1.2 0.00106 430049.15625 CID (Collision Induced Dissociation) MS2 0.0 70 24.5 60.0 103.9988 34213 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 9.7 19.7 33.2 11.1 15.1 38.3 16.6 27.3 49.0 24.7 2.04 1 0.002304 0.01406 False High Sequest HT (A2) Unambiguous FGLSPSK [K].fGLsPSk.[G] N-Term(TMT6plex); S4(Phospho); K7(TMT6plex) 1 P25694 P25694 0 2 0.2076 0 1 1 637.35132 1273.69537 1273.69576 -0.31 -0.0002 6827861.0 CID (Collision Induced Dissociation) MS2 4.639447 20 121.5 39.93 87.4461 27964 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 33.2 91.6 190.2 38.1 103.3 223.4 44.7 110.8 249.2 131.0 Excluded by Method 2.89 1 0.002304 0.0163 False High Sequest HT (A2) Unambiguous AVGPASILK [K].aVGPAsILk.[E] N-Term(TMT6plex); S6(Phospho); K9(TMT6plex) 2 O95218 O95218-2; O95218 0 2 0.3953 0 1 1 697.41455 1393.82182 1393.82202 -0.15 -0.0001 718864.3125 CID (Collision Induced Dissociation) MS2 56.9486 40 42.4 60.0 95.8206 31192 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 44.2 42.8 36.5 47.4 41.6 39.6 39.7 44.7 51.0 36.4 Excluded by Method 2.96 1 0.002304 0.01596 False High Sequest HT (A2) Unambiguous TSTSPPPEK [K].tSTsPPPEk.[S] N-Term(TMT6plex); S4(Phospho); K9(TMT6plex) 2 Q9H1E3 Q9H1E3; Q9H1E3-2 0 3 0.0472 0 1 1 494.59359 1481.76621 1481.7653 0.61 0.0003 2068522.625 CID (Collision Induced Dissociation) MS2 4.369602 40 119.8 60.0 45.8447 12438 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 111.8 116.0 101.3 119.9 117.9 121.4 131.9 127.8 119.3 130.3 Excluded by Method 2.12 1 0.002304 0.01468 False High Sequest HT (A2) Unambiguous NTLETSSLNFK [K].nTLETSsLNFk.[V] N-Term(TMT6plex); S7(Phospho); K11(TMT6plex) 1 Q14151 Q14151 0 3 0.0492 0 1 1 597.98174 1791.93068 1791.9294 0.71 0.00042 1013820.1875 CID (Collision Induced Dissociation) MS2 11.10757 50 83.4 60.0 94.1078 30495 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 77.3 84.8 62.3 93.8 76.4 91.3 87.8 85.9 90.5 84.3 Excluded by Method 3.25 1 0.002304 0.01373 False High Sequest HT (A2) Unambiguous LTFDTTFSPNTGKK [K].lTFDTTFsPNTGkk.[S] N-Term(TMT6plex); S8(Phospho); K13(TMT6plex); K14(TMT6plex) 3 P45880 P45880; P45880-2; P45880-1 1 4 0.0629 0 1 1 581.81789 2324.24971 2324.25063 -0.39 -0.00023 1169987.25 CID (Collision Induced Dissociation) MS2 0.0 40 99.0 60.0 102.3603 33593 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 98.7 96.6 86.1 90.3 101.6 101.9 110.4 103.7 102.3 98.0 Excluded by Method 3.02 1 0.002304 0.01308 False High Sequest HT (A2) Unambiguous IAASLSDDDLK [K].iAASLsDDDLk.[E] N-Term(TMT6plex); S6(Phospho); K11(TMT6plex) 1 P25389 P25389 0 3 0.1033 0 1 1 562.63033 1685.87643 1685.8763 0.07 4e-05 1049211.375 CID (Collision Induced Dissociation) MS2 19.13866 30 23.8 60.0 97.1318 31687 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 6.0 19.0 31.1 9.4 24.6 45.2 9.8 19.4 51.8 22.1 Excluded by Method 2.42 1 0.002304 0.01513 False High Sequest HT (A2) Unambiguous WLISPVK [K].wLIsPVk.[I] N-Term(TMT6plex); S4(Phospho); K7(TMT6plex) 3 Q8NCD3 Q8NCD3-2; Q8NCD3-3; Q8NCD3 0 3 0.4687 0 1 1 460.9399 1380.80515 1380.80565 -0.36 -0.00017 506770.46875 CID (Collision Induced Dissociation) MS2 0.0 40 127.5 60.0 119.693 40002 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 142.5 128.7 95.0 132.7 117.0 131.5 146.9 135.2 124.9 120.8 Excluded by Method 2.88 1 0.002304 0.01505 False High Sequest HT (A2) Unambiguous QGGSPDEPDSK [K].qGGsPDEPDSk.[A] N-Term(TMT6plex); S4(Phospho); K11(TMT6plex) 2 Q9ULX6 Q9ULX6; Q9ULX6-2 0 2 0.6258 0 1 1 827.89019 1654.7731 1654.77257 0.32 0.00026 102141.8515625 CID (Collision Induced Dissociation) MS2 0.0 60 25.1 60.0 39.4009 10157 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 21.8 24.9 23.8 22.8 25.7 22.4 32.7 28.5 24.8 23.9 Excluded by Method 3.02 1 0.002304 0.01371 False High Sequest HT (A2) Unambiguous VLSDSEDEEK [K].vLsDsEDEEk.[D] N-Term(TMT6plex); S3(Phospho); S5(Phospho); K10(TMT6plex) 1 Q8WWQ0 Q8WWQ0 0 3 0.244 0 1 1 590.26126 1768.76923 1768.76953 -0.17 -0.0001 289397.0625 CID (Collision Induced Dissociation) MS2 41.36313 30 46.7 60.0 73.5169 22717 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 42.3 51.0 39.1 45.0 43.9 48.1 50.9 49.6 61.0 36.2 Excluded by Method 2.5 1 0.002424 0.01842 False High Sequest HT (A2) Unambiguous RSASYTGAKV [K].rSAsYTGAkV.[-] N-Term(TMT6plex); S4(Phospho); K9(TMT6plex) 1 P31688 P31688 2 3 0.1039 0 1 1 526.61987 1577.84505 1577.84528 -0.15 -8e-05 3898002.25 CID (Collision Induced Dissociation) MS2 27.21474 40 76.0 35.083 49.4354 13801 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 23.2 51.4 115.4 23.7 63.1 148.9 31.7 69.3 150.6 82.6 Excluded by Method 2.31 1 0.002424 0.0191 False High Sequest HT (A2) Unambiguous VGILPSSSTDDVTSK [K].vGILPSSStDDVTSk.[E] N-Term(TMT6plex); T9(Phospho); K15(TMT6plex) 1 P22215 P22215 0 3 0.0465 0 1 1 682.0247 2044.05956 2044.06154 -0.97 -0.00066 1202298.0 CID (Collision Induced Dissociation) MS2 11.06668 60 159.3 60.0 98.8368 32248 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 68.5 142.8 219.1 66.1 134.6 282.6 82.2 158.6 273.9 164.4 Excluded by Method 3.01 1 0.002424 0.01917 False High Sequest HT (A2) Unambiguous EIPASPSRTK [K].eIPASPsRTk.[I] N-Term(TMT6plex); S7(Phospho); K10(TMT6plex) 1 Q9NYZ3 Q9NYZ3 1 3 0.0216 0 1 1 541.9672 1623.88705 1623.88714 -0.06 -3e-05 1094026.5 CID (Collision Induced Dissociation) MS2 12.43401 20 96.3 60.0 52.3309 14873 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 103.2 106.2 81.4 89.2 89.7 96.5 102.5 97.4 94.9 102.0 Excluded by Method 2.78 1 0.002424 0.0188 False High Sequest HT (A2) Unambiguous WFFISGSV [K].wFFISGsV.[-] N-Term(TMT6plex); S7(Phospho) 1 P00817 P00817 0 2 0.25 0 1 1 626.30435 1251.60141 1251.60124 0.14 9e-05 357438.1875 CID (Collision Induced Dissociation) MS2 0.0 50 36.7 60.0 135.8616 46344 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 11.8 28.2 55.6 14.7 26.8 69.7 18.0 38.0 68.6 35.4 Excluded by Method 2.16 1 0.002424 0.01874 False High Sequest HT (A2) Unambiguous DDSHSAEDSEDEK [K].dDSHSAEDsEDEk.[E] N-Term(TMT6plex); S9(Phospho); K13(TMT6plex) 2 Q9H1E3 Q9H1E3; Q9H1E3-2 0 2 0.3108 0 1 1 1001.42 2001.83272 2001.83266 0.03 3e-05 102473.5703125 CID (Collision Induced Dissociation) MS2 0.0 50 6.2 60.0 31.353 7232 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 12.0 3.4 5.9 4.2 4.1 6.8 8.9 4.0 6.4 6.7 Excluded by Method 2.22 1 0.002424 0.01821 False High Sequest HT (A2) Unambiguous REGPVGGESDSEEMFEK [K].rEGPVGGEsDSEEMFEk.[T] N-Term(TMT6plex); S9(Phospho); K17(TMT6plex) 3 Q9Y4B5 Q9Y4B5-3; Q9Y4B5-2; Q9Y4B5 1 3 0.25 0 1 1 807.70673 2421.10563 2421.10454 0.45 0.00036 1646616.125 CID (Collision Induced Dissociation) MS2 0.0 60 16.2 60.0 80.7079 25423 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 14.7 15.9 20.2 14.3 14.3 19.4 14.5 15.0 19.5 13.8 Excluded by Method 2.32 1 0.002424 0.01936 False High Sequest HT (A2) Unambiguous HEQPQIESPK [K].hEQPQIEsPk.[-] N-Term(TMT6plex); S8(Phospho); K10(TMT6plex) 1 P40558 P40558 0 3 0.3214 0 1 1 577.63338 1730.88559 1730.88787 -1.32 -0.00076 303024.1875 CID (Collision Induced Dissociation) MS2 38.02866 40 31.2 60.0 45.7417 12440 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 13.2 25.0 40.5 14.2 30.8 59.4 16.1 25.9 57.3 29.7 Excluded by Method 1.96 1 0.002424 0.01844 False High Sequest HT (A2) Unambiguous DSTLIMQLLRDNLTLWTSENQGDEGDAGEGEN [K].dSTLImQLLRDNLTLWTSENQGDEGDAGEGEN.[-] N-Term(TMT6plex); M6(Oxidation) 2 P31946 P31946-2; P31946 1 4 0.4116 0 1 1 942.44507 3766.75845 3766.75512 0.88 0.00083 342802.4375 CID (Collision Induced Dissociation) MS2 0.0 40 4.9 60.0 136.396 46528 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 3.4 3.1 3.8 3.3 6.5 11.1 3.6 2.8 8.1 3.6 Excluded by Method 3.62 1 0.002424 0.01891 False High Sequest HT (A2) Unambiguous LSRGDSLK [K].lSRGDsLk.[E] N-Term(TMT6plex); S6(Phospho); K8(TMT6plex) 2 Q9C0B5 Q9C0B5; Q9C0B5-2 1 3 0.2313 0 1 1 471.93374 1413.78666 1413.7867 -0.03 -1e-05 2002601.875 CID (Collision Induced Dissociation) MS2 6.212742 20 205.0 60.0 56.011 16243 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 197.8 202.9 160.9 218.3 193.9 202.1 234.1 225.8 210.5 203.5 Excluded by Method 2.68 1 0.002424 0.0181 False High Sequest HT (A2) Unambiguous NTLETSSLNFK [K].nTLEtSSLNFk.[V] N-Term(TMT6plex); T5(Phospho); K11(TMT6plex) 1 Q14151 Q14151 0 3 0.0116 0 1 1 597.98132 1791.92939 1791.9294 0.0 0.0 1869732.375 CID (Collision Induced Dissociation) MS2 5.999073 40 67.1 55.27 98.3772 32101 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 49.3 70.4 59.6 56.5 62.4 86.3 65.6 69.3 78.0 73.9 Excluded by Method 2.59 1 0.002424 0.01849 False High Sequest HT (A2) Unambiguous LETDESPIQTK [K].lETDEsPIQTk.[S] N-Term(TMT6plex); S6(Phospho); K11(TMT6plex) 1 P23585 P23585 0 3 0.2389 0 1 1 600.31286 1798.92401 1798.92398 0.02 1e-05 2096513.0 CID (Collision Induced Dissociation) MS2 46.34478 30 42.4 60.0 75.434 23474 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 27.9 34.0 41.7 38.7 42.9 53.0 41.3 46.8 50.3 46.9 Excluded by Method 1.8 1 0.002424 0.01935 False High Sequest HT (A2) Unambiguous IEEVLSPEGSPSKSPSK [K].iEEVLSPEGSPSksPSk.[K] N-Term(TMT6plex); K13(TMT6plex); S14(Phospho); K17(TMT6plex) 2 Q9UEY8 Q9UEY8-2; Q9UEY8 1 3 0.0369 0 1 1 846.79354 2538.36607 2538.36711 -0.41 -0.00035 3079077.0 CID (Collision Induced Dissociation) MS2 0.0 50 27.0 39.912 92.7314 30032 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 29.1 23.6 20.2 28.4 24.4 31.0 26.0 29.4 30.7 27.1 Excluded by Method 2.44 1 0.002424 0.0188 False High Sequest HT (A2) Unambiguous KEESEESDDDMGFGLFD [K].kEESEEsDDDmGFGLFD.[-] N-Term(TMT6plex); K1(TMT6plex); S7(Phospho); M11(Oxidation) 3 P05387 P05386; P05386-2; P05387 1 2 0.1604 0 1 1 1252.53043 2504.05357 2504.04642 2.86 0.00358 430459.65625 CID (Collision Induced Dissociation) MS2 0.0 30 28.9 60.0 114.7164 38205 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 31.5 28.1 17.2 33.9 26.5 27.0 30.6 29.9 29.9 34.8 Excluded by Method 2.12 1 0.002424 0.01854 False High Sequest HT (A2) Unambiguous FGLSPSK [K].fGLsPSk.[G] N-Term(TMT6plex); S4(Phospho); K7(TMT6plex) 1 P25694 P25694 0 2 0.2 0 1 1 637.35168 1273.69609 1273.69576 0.26 0.00017 5774734.5 CID (Collision Induced Dissociation) MS2 0.0 20 114.6 29.754 86.4666 27618 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 30.3 94.9 186.0 41.6 90.5 220.1 44.1 107.0 225.2 105.8 Excluded by Method 2.55 1 0.002424 0.01796 False High Sequest HT (A2) Unambiguous IFGSRVRVDSTAK [K].iFGSRVRVDsTAk.[V] N-Term(TMT6plex); S10(Phospho); K13(TMT6plex) 2 P78371 P78371-2; P78371 2 4 0.1238 0 1 1 494.27936 1974.09559 1974.09378 0.92 0.00045 461613.78125 CID (Collision Induced Dissociation) MS2 0.0 10 65.8 60.0 72.5308 22438 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 57.0 65.6 49.4 75.2 66.1 65.1 66.8 72.7 71.4 68.3 Excluded by Method 2.02 1 0.002424 0.01917 False High Sequest HT (A2) Unambiguous SYQNSPSSDDGIRPLPEYSTEK [K].sYQNsPsSDDGIRPLPEYSTEk.[H] N-Term(TMT6plex); S5(Phospho); S7(Phospho); K22(TMT6plex) 1 Q15648 Q15648 0 3 0.0356 0 1 1 1030.13692 3088.39622 3088.39538 0.27 0.00028 490219.09375 CID (Collision Induced Dissociation) MS2 0.0 50 51.1 60.0 91.1181 29373 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 43.3 49.1 44.3 50.9 46.8 53.1 56.8 59.2 50.7 56.5 Excluded by Method 3.37 1 0.002424 0.01866 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVsSDEEF.[-] N-Term(TMT6plex); S8(Phospho) 1 P16521 P16521 0 2 0.0362 0 1 1 885.37186 1769.73644 1769.73562 0.46 0.00041 296194.28125 CID (Collision Induced Dissociation) MS2 39.60504 40 38.4 60.0 139.8615 47712 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 11.6 34.9 58.0 10.2 31.0 72.5 16.8 36.4 64.4 48.4 Excluded by Method 2.76 1 0.002424 0.0191 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVssDEEF.[-] N-Term(TMT6plex); S8(Phospho); S9(Phospho) 1 P16521 P16521 0 2 0.2476 0 1 1 925.35516 1849.70305 1849.70195 0.59 0.00055 355970.46875 CID (Collision Induced Dissociation) MS2 40.80233 40 47.9 60.0 136.0336 46400 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 18.3 44.9 73.1 19.0 40.0 85.6 21.6 36.0 94.4 46.0 Excluded by Method 2.1 1 0.002424 0.01868 False High Sequest HT (A2) Unambiguous AFSIIPSQRFIK [K].aFSIIPsQRFIk.[S] N-Term(TMT6plex); S7(Phospho); K12(TMT6plex) 1 P38970 P38970 1 3 0.4832 0 1 1 649.04167 1945.11046 1945.10764 1.45 0.00094 237438.265625 CID (Collision Induced Dissociation) MS2 29.40874 30 68.8 60.0 118.0324 39386 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 17.6 58.4 111.4 20.8 57.3 134.2 21.2 59.4 133.7 73.7 Excluded by Method 3.58 1 0.002424 0.01811 False High Sequest HT (A2) Unambiguous SFKLSGFSFK [K].sFkLsGFSFk.[K] N-Term(TMT6plex); K3(TMT6plex); S5(Phospho); K10(TMT6plex) 1 P29966 P29966 1 3 0.1802 0 1 1 639.0282 1915.07005 1915.06975 0.16 0.0001 954254.0 CID (Collision Induced Dissociation) MS2 2.822912 10 279.9 60.0 120.3626 40251 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 295.4 280.2 233.1 310.2 247.7 266.9 352.7 307.0 269.4 236.5 Excluded by Method 2.83 1 0.002424 0.0192 False High Sequest HT (A2) Unambiguous RISICSSDK [K].rIsIcSSDk.[R] N-Term(TMT6plex); S3(Phospho); C5(Carbamidomethyl); K9(TMT6plex) 1 Q13547 Q13547 1 3 0.0667 0 1 1 535.28108 1603.82869 1603.82791 0.49 0.00026 863836.75 CID (Collision Induced Dissociation) MS2 17.32831 20 96.9 60.0 61.0474 18088 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 74.0 89.2 109.2 83.0 94.6 125.1 74.6 95.2 130.9 92.9 Excluded by Method 1.35 1 0.002424 0.01823 False High Sequest HT (A2) Unambiguous FGSADNIPNLK [K].fGsADNIPNLk.[D] N-Term(TMT6plex); S3(Phospho); K11(TMT6plex) 2 O94876 O94876-2; O94876 0 3 0.3006 0 1 1 571.97016 1713.89593 1713.89771 -1.04 -0.00059 461383.9375 CID (Collision Induced Dissociation) MS2 0.0 60 159.4 60.0 105.5267 34829 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 159.7 161.2 119.8 188.1 145.6 152.3 184.4 168.5 166.5 147.8 Excluded by Method 3.26 1 0.002424 0.01894 False High Sequest HT (A2) Unambiguous ARASPSVTCK [K].aRAsPSVTck.[S] N-Term(TMT6plex); S4(Phospho); C9(Carbamidomethyl); K10(TMT6plex) 1 O15014 O15014 1 3 0.3728 0 1 1 538.95417 1614.84797 1614.8439 2.52 0.00136 557369.25 CID (Collision Induced Dissociation) MS2 8.345433 60 22.6 60.0 42.0728 11115 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 24.1 20.6 14.7 22.2 24.7 23.5 25.5 25.3 20.7 24.8 Excluded by Method 2.28 1 0.002424 0.01922 False High Sequest HT (A2) Unambiguous TSVESEIGGNQVGLK [K].tSVEsEIGGNQVGLk.[I] N-Term(TMT6plex); T1(Phospho); S5(Phospho); K15(TMT6plex) 1 P34250 P34250 0 3 0.0056 0 1 1 712.6851 2136.04075 2136.0391 0.77 0.00055 864258.4375 CID (Collision Induced Dissociation) MS2 26.19976 30 61.1 60.0 100.6727 33017 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 34.6 52.2 85.6 37.5 52.4 89.2 45.0 58.3 94.2 61.7 Excluded by Method 1.79 1 0.002574 0.01966 False High Sequest HT (A2) Unambiguous ELSPAGSISK [K].eLsPAGsISk.[N] N-Term(TMT6plex); S3(Phospho); S7(Phospho); K10(TMT6plex) 4 O95453 O95453-4; O95453-2; O95453-3; O95453 0 3 0.2678 0 1 1 536.26789 1606.78911 1606.78948 -0.23 -0.00012 648869.125 CID (Collision Induced Dissociation) MS2 35.33751 20 24.4 60.0 78.1793 24480 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 21.3 22.7 20.3 26.2 20.7 29.7 24.5 25.7 32.5 20.8 Excluded by Method 1.83 1 0.002716 0.01984 False High Sequest HT (A2) Unambiguous SEPVSPQDFSFSK [K].sEPVSPQDFSFSk.[N] N-Term(TMT6plex); S1(Phospho); K13(TMT6plex) 1 P48552 P48552 0 3 0.006 0 1 1 664.99574 1992.97268 1992.972 0.34 0.00023 418262.5 CID (Collision Induced Dissociation) MS2 41.5412 60 22.7 60.0 106.5648 35136 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 18.6 18.7 24.5 17.5 16.1 31.4 17.5 24.9 35.1 22.3 Excluded by Method 1.68 1 0.002716 0.02008 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVSsDEEF.[-] N-Term(TMT6plex); S9(Phospho) 1 P16521 P16521 0 2 0.0904 0 1 1 885.37614 1769.74501 1769.73562 5.3 0.00469 1629748.625 CID (Collision Induced Dissociation) MS2 0.0 40 94.0 27.225 116.9149 39051 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 23.8 75.2 152.1 31.5 71.7 187.2 32.4 91.7 180.6 94.2 Excluded by Method 3.65 1 0.002716 0.02001 False High Sequest HT (A2) Unambiguous ELEAERSLMSSPEDLTK [K].eLEAERSLMssPEDLTk.[D] N-Term(TMT6plex); S10(Phospho); S11(Phospho); K17(TMT6plex) 1 P46821 P46821 1 3 0.3711 0 1 1 851.73793 2553.19924 2553.19607 1.24 0.00106 539045.4375 CID (Collision Induced Dissociation) MS2 8.828223 20 97.1 60.0 108.3624 35868 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 105.2 99.9 75.4 99.4 94.5 100.9 107.5 98.8 91.6 98.0 Excluded by Method 3.53 1 0.002716 0.02017 False High Sequest HT (A2) Unambiguous LSGFSFK [K].lSGFsFk.[K] N-Term(TMT6plex); S5(Phospho); K7(TMT6plex) 1 P29966 P29966 0 2 0.4157 0 1 1 662.35934 1323.71141 1323.71141 0.0 0.0 6430168.0 CID (Collision Induced Dissociation) MS2 1.220706 10 147.6 24.941 112.7462 37477 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 150.1 155.2 116.6 147.6 146.2 136.2 160.0 164.0 151.7 148.4 Excluded by Method 2.55 1 0.002716 0.01994 False High Sequest HT (A2) Unambiguous ELDTAQK [K].eLDTAQk.[H] N-Term(TMT6plex); K7(TMT6plex) 3 P00359 P00360; P00359; P00358 0 2 0.3984 0 1 1 631.87269 1262.7381 1262.73564 1.95 0.00123 524694.25 CID (Collision Induced Dissociation) MS2 30.53722 40 50.2 60.0 49.5963 13862 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 28.0 37.9 61.7 20.8 42.5 88.6 31.3 51.8 75.8 63.2 Excluded by Method 2.46 1 0.002845 0.02131 False High Sequest HT (A2) Unambiguous SPVISRPK [K].sPVISRPk.[S] N-Term(TMT6plex); S1(Phospho); K8(TMT6plex) 2 Q8N8S7-2 Q8N8S7-3; Q8N8S7-2 0 3 0.3234 0 1 1 474.61429 1421.82833 1421.82817 0.11 5e-05 6329871.5 CID (Collision Induced Dissociation) MS2 4.942333 20 127.4 36.662 50.9758 14348 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 141.5 131.8 103.3 129.9 119.0 118.2 134.5 135.9 115.5 144.3 Excluded by Method 2.35 1 0.002845 0.02141 False High Sequest HT (A2) Unambiguous RGFEGSCSQK [K].rGFEGScsQk.[E] N-Term(TMT6plex); C7(Carbamidomethyl); S8(Phospho); K10(TMT6plex) 1 O43719 O43719 1 3 0.1038 0 1 1 565.27583 1693.81294 1693.81333 -0.23 -0.00013 1455302.5 CID (Collision Induced Dissociation) MS2 12.13709 50 63.4 60.0 43.9131 11776 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 68.4 64.0 53.5 58.8 60.4 68.4 71.4 65.8 56.4 67.0 Excluded by Method 2.6 1 0.002845 0.02156 False High Sequest HT (A2) Unambiguous FSVSSNDSQNSLK [K].fSVSSNDsQNsLk.[N] N-Term(TMT6plex); S8(Phospho); S11(Phospho); K13(TMT6plex) 1 Q06833 Q06833 0 3 0.1744 0 1 1 677.64681 2030.92587 2030.92374 1.05 0.00071 1498903.25 CID (Collision Induced Dissociation) MS2 24.08007 20 60.3 60.0 89.9336 28977 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 28.1 50.4 77.3 28.8 48.6 101.8 37.5 59.7 114.3 56.2 Excluded by Method 2.58 1 0.002845 0.02128 False High Sequest HT (A2) Unambiguous DMSPLSETEMALGK [K].dMSPLsETEMALGk.[D] N-Term(TMT6plex); S6(Phospho); K14(TMT6plex) 3 P27816 P27816; P27816-6; P27816-2 0 3 0.0446 0 1 1 683.00166 2046.99043 2046.9893 0.55 0.00038 913110.5625 CID (Collision Induced Dissociation) MS2 26.2236 10 75.9 60.0 126.7252 42665 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 80.4 76.9 59.6 73.4 70.3 76.2 89.7 80.2 79.1 72.8 Excluded by Method 2.02 1 0.002845 0.02089 False High Sequest HT (A2) Unambiguous WFFISGSV [K].wFFISGsV.[-] N-Term(TMT6plex); S7(Phospho) 1 P00817 P00817 0 2 0.1667 0 1 1 626.30459 1251.6019 1251.60124 0.53 0.00033 193034.5625 CID (Collision Induced Dissociation) MS2 0.0 50 24.8 60.0 145.1021 49561 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 7.3 21.5 39.5 6.8 17.2 47.5 9.6 24.5 46.4 27.2 Excluded by Method 1.74 1 0.002845 0.02066 False High Sequest HT (A2) Unambiguous SDISPLTPRESSPLYSPTFSDSTSAVK [K].sDISPLTPRESsPLYSPTFSDSTSAVk.[E] N-Term(TMT6plex); S12(Phospho); K27(TMT6plex) 1 P46821 P46821 1 4 0.0286 0 1 1 852.67983 3407.69747 3407.70237 -1.44 -0.00122 529607.9375 CID (Collision Induced Dissociation) MS2 22.193 20 36.0 60.0 110.6059 36710 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 36.7 30.3 34.3 35.4 36.2 39.7 43.3 30.6 36.7 36.4 Excluded by Method 2.45 1 0.002845 0.02103 False High Sequest HT (A2) Unambiguous ADTTTPTTSAITASRSESPPPLSDPK [K].aDTTTPTTSAITASRSEsPPPLSDPk.[Q] N-Term(TMT6plex); S18(Phospho); K26(TMT6plex) 2 Q15059 Q15059; Q15059-2 1 3 0.1036 0 1 1 1056.2025 3166.59295 3166.59209 0.27 0.00029 663011.5625 CID (Collision Induced Dissociation) MS2 0.0 30 67.8 60.0 81.3598 25759 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 61.2 65.7 59.3 61.6 66.6 74.6 82.6 64.9 74.2 67.7 Excluded by Method 1.93 1 0.002845 0.02106 False High Sequest HT (A2) Unambiguous TASESISNLSEAGSIK [K].tASESISNLsEAGsIk.[K] N-Term(TMT6plex); S10(Phospho); S14(Phospho); K16(TMT6plex) 3 P30622 P30622-2; P30622; P30622-1 0 3 0.2768 0 1 1 738.02422 2212.0581 2212.05515 1.34 0.00099 1096625.75 CID (Collision Induced Dissociation) MS2 27.05496 20 215.9 44.51 89.9217 28917 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 91.4 180.7 306.0 103.7 167.0 360.1 123.2 206.1 401.7 219.4 Excluded by Method 2.71 1 0.002845 0.02043 False High Sequest HT (A2) Unambiguous VLPELQGK [K].vLPELQGk.[L] N-Term(TMT6plex); K8(TMT6plex) 3 P00359 P00360; P00359; P00358 0 2 0.3924 0 1 1 671.42946 1341.85164 1341.85061 0.77 0.00052 808591.8125 CID (Collision Induced Dissociation) MS2 12.96552 50 136.4 60.0 89.4122 28724 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 46.2 118.1 216.6 55.7 128.4 253.8 55.1 118.9 234.8 136.5 Excluded by Method 2.37 1 0.002845 0.02157 False High Sequest HT (A2) Unambiguous SANEFSTTPEDK [K].sANEFSTtPEDk.[H] N-Term(TMT6plex); T8(Phospho); K12(TMT6plex) 1 Q06315 Q06315 0 3 0.2597 0 1 1 621.96425 1863.87821 1863.87776 0.24 0.00015 2510732.0 CID (Collision Induced Dissociation) MS2 0.0 50 29.5 39.275 64.5958 19409 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 15.6 25.6 34.4 16.5 25.2 46.0 22.7 27.7 49.9 31.6 Excluded by Method 1.81 1 0.002845 0.02065 False High Sequest HT (A2) Unambiguous EISVSQK [K].eIsVSQk.[A] N-Term(TMT6plex); S3(Phospho); K7(TMT6plex) 1 Q05050 Q05050 0 2 0.0826 0 1 1 664.8652 1328.72312 1328.7227 0.31 0.00021 1609338.875 CID (Collision Induced Dissociation) MS2 0.0 20 48.0 60.0 63.7369 19091 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 21.4 36.5 62.3 17.6 41.2 88.3 22.8 46.3 87.8 55.9 Excluded by Method 2.42 1 0.002845 0.02033 False High Sequest HT (A2) Unambiguous TSTSPPPEK [K].tSTsPPPEk.[S] N-Term(TMT6plex); S4(Phospho); K9(TMT6plex) 2 Q9H1E3 Q9H1E3; Q9H1E3-2 0 2 0.1463 0 1 1 741.38634 1481.76539 1481.7653 0.07 5e-05 1479510.25 CID (Collision Induced Dissociation) MS2 10.80371 20 407.7 60.0 44.2514 11900 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 422.3 381.7 340.9 416.2 399.5 413.7 454.1 431.6 416.8 400.1 Excluded by Method 2.94 1 0.002845 0.0206 False High Sequest HT (A2) Unambiguous LQALPTP [K].lQALPtP.[-] N-Term(TMT6plex); T6(Phospho) 1 Q06188 Q06188 0 2 0.7056 0 1 1 524.78561 1048.56395 1048.56413 -0.17 -9e-05 404544.28125 CID (Collision Induced Dissociation) MS2 0.0 40 100.7 60.0 100.34 32835 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 35.3 85.9 165.5 45.7 78.5 188.0 37.7 89.9 178.6 101.7 Excluded by Method 1.97 1 0.002845 0.02088 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVssDEEF.[-] N-Term(TMT6plex); S8(Phospho); S9(Phospho) 1 P16521 P16521 0 2 0.2157 0 1 1 925.35618 1849.70509 1849.70195 1.69 0.00157 271366.09375 CID (Collision Induced Dissociation) MS2 0.0 30 54.8 60.0 134.4775 45842 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 13.4 41.1 86.0 18.2 43.0 104.3 20.1 49.5 115.2 56.7 Excluded by Method 2.04 1 0.002845 0.02146 False High Sequest HT (A2) Unambiguous LSGFSFK [K].lSGFsFk.[K] N-Term(TMT6plex); S5(Phospho); K7(TMT6plex) 1 P29966 P29966 0 2 0.4491 0 1 1 662.35959 1323.7119 1323.71141 0.37 0.00024 420099.78125 CID (Collision Induced Dissociation) MS2 21.47424 10 21.1 60.0 120.2427 40244 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 21.1 16.9 19.4 20.9 17.6 21.9 25.4 22.8 23.1 22.1 Excluded by Method 2.16 1 0.002845 0.02096 False High Sequest HT (A2) Unambiguous LYERRGGGSGGGEESEGEEVDED [K].lYERRGGGSGGGEEsEGEEVDED.[-] N-Term(TMT6plex); S15(Phospho) 1 Q96QR8 Q96QR8 2 3 0.3011 0 1 1 908.04901 2722.13249 2722.13147 0.37 0.00034 2126667.75 CID (Collision Induced Dissociation) MS2 0.0 60 53.5 60.0 54.028 15447 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 54.0 54.0 51.8 52.5 43.3 55.2 57.8 52.5 59.7 53.7 Excluded by Method 2.79 1 0.002845 0.02026 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVssDEEF.[-] N-Term(TMT6plex); S8(Phospho); S9(Phospho) 1 P16521 P16521 0 2 0.1515 0 1 1 925.35478 1849.70228 1849.70195 0.18 0.00016 502352.375 CID (Collision Induced Dissociation) MS2 4.34313 30 56.7 60.0 148.8338 50813 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 14.9 43.3 91.2 19.8 42.8 115.5 17.8 52.6 111.7 57.5 Excluded by Method 2.31 1 0.00299 0.02175 False High Sequest HT (A2) Unambiguous YDSPVSSPITSASELGSIAK [K].yDsPVsSPITSASELGSIAk.[L] N-Term(TMT6plex); S3(Phospho); S6(Phospho); K20(TMT6plex) 1 P53890 P53890 0 3 0.2318 0 1 1 876.42851 2627.27099 2627.26587 1.95 0.00171 539915.6875 CID (Collision Induced Dissociation) MS2 70.44923 40 73.8 60.0 125.5834 42199 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 16.0 54.0 114.1 27.1 57.6 146.4 22.9 69.3 159.0 71.4 Excluded by Method 2.2 1 0.00299 0.02166 False High Sequest HT (A2) Unambiguous STFREESPLRIK [K].sTFREEsPLRIk.[M] N-Term(TMT6plex); S7(Phospho); K12(TMT6plex) 4 Q9NYF8 Q9NYF8-3; Q9NYF8-4; Q9NYF8; Q9NYF8-2 2 4 0.3395 0 1 1 501.02874 2001.09312 2001.09345 -0.16 -8e-05 5339675.5 CID (Collision Induced Dissociation) MS2 6.550855 0 132.0 27.225 73.729 22892 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 119.2 140.1 120.9 142.1 123.0 130.9 152.0 135.2 133.4 123.6 Excluded by Method 1.62 1 0.00299 0.02167 False High Sequest HT (A2) Unambiguous NPDEDEFLINSDDEM [K].nPDEDEFLINsDDEm.[-] N-Term(TMT6plex); S11(Phospho); M15(Oxidation) 1 Q08412 Q08412 0 2 0.6963 0 1 1 1054.42461 2107.84195 2107.82524 7.92 0.00835 296910.5 CID (Collision Induced Dissociation) MS2 45.97418 20 151.9 60.0 88.678 28448 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 30.8 124.4 245.4 50.1 129.2 292.3 48.9 140.2 300.9 157.0 Excluded by Method 1.91 1 0.0031 0.02329 False High Sequest HT (A2) Unambiguous SPSLSPSPPSPLEK [K].sPSLSPSPPSPLEk.[T] N-Term(TMT6plex); S1(Phospho); K14(TMT6plex) 1 P46821 P46821 0 3 0.0181 0 1 1 654.35111 1961.03879 1961.03968 -0.46 -0.0003 1780070.875 CID (Collision Induced Dissociation) MS2 17.27352 20 106.3 39.525 91.2663 29429 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 107.5 101.5 92.4 103.4 94.3 102.8 118.3 116.4 106.3 120.4 Excluded by Method 2.76 1 0.0031 0.02421 False High Sequest HT (A2) Unambiguous EECPRPMSISPPDFSPK [K].eEcPRPmSISPPDFsPk.[T] N-Term(TMT6plex); C3(Carbamidomethyl); M7(Oxidation); S15(Phospho); K17(TMT6plex) 1 P46821 P46821 0 3 0.3042 0 1 1 843.405 2528.20044 2528.19667 1.49 0.00126 415798.4375 CID (Collision Induced Dissociation) MS2 47.20213 30 56.5 60.0 84.4261 26853 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 57.3 56.7 48.1 52.8 51.0 58.3 61.4 61.4 63.4 54.1 Excluded by Method 2.4 1 0.0031 0.02303 False High Sequest HT (A2) Unambiguous ELNVSFINEK [K].eLNVsFINEk.[KR] N-Term(TMT6plex); S5(Phospho); K10(TMT6plex) 5 P49585 P49585; Q9Y5K3; Q9Y5K3-3; Q9Y5K3-2; Q9Y5K3-4 0 3 0.1994 0 1 1 577.64306 1730.91462 1730.91302 0.92 0.00053 1336809.625 CID (Collision Induced Dissociation) MS2 7.14546 70 49.3 60.0 112.8769 37492 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 46.7 43.6 39.3 47.6 46.3 56.8 60.1 51.0 55.5 45.7 3.21 1 0.0031 0.024 False High Sequest HT (A2) Unambiguous KLNELQNSEDSDAEDGGK [K].kLNELQNSEDsDAEDGGk.[Q] N-Term(TMT6plex); K1(TMT6plex); S11(Phospho); K18(TMT6plex) 1 Q12136 Q12136 1 3 0.083 0 1 1 906.11585 2716.333 2716.32816 1.78 0.00161 371766.46875 CID (Collision Induced Dissociation) MS2 0.0 60 9.0 60.0 70.2151 21507 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 6.5 8.1 9.8 3.7 4.1 15.3 8.6 9.2 13.1 11.7 Excluded by Method 2.29 1 0.0031 0.02368 False High Sequest HT (A2) Unambiguous VGEGTYGVVYK [K].vGEGtYGVVYk.[A] N-Term(TMT6plex); T5(Phospho); K11(TMT6plex) 1 P00546 P00546 0 3 0.0302 0 1 1 570.63471 1709.88959 1709.89156 -1.15 -0.00066 801333.5625 CID (Collision Induced Dissociation) MS2 8.961745 50 34.6 60.0 82.16 26065 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 23.5 30.0 35.1 21.1 29.9 46.4 34.9 33.3 53.2 38.8 Excluded by Method 2.32 1 0.0031 0.02393 False High Sequest HT (A2) Unambiguous VATLNSEEESDPPTYK [K].vATLNsEEESDPPTYk.[D] N-Term(TMT6plex); S6(Phospho); K16(TMT6plex) 2 Q00341 Q00341; Q00341-2 0 2 0.1131 0 1 1 1159.56531 2318.12333 2318.12051 1.22 0.00141 258793.21875 CID (Collision Induced Dissociation) MS2 0.0 40 42.8 60.0 83.672 26637 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 41.0 43.0 36.3 43.9 44.2 43.9 44.8 44.8 42.8 43.2 Excluded by Method 2.74 1 0.0031 0.02405 False High Sequest HT (A2) Unambiguous TPSPKEEDEEPESPPEK [K].tPsPkEEDEEPEsPPEk.[K] N-Term(TMT6plex); S3(Phospho); K5(TMT6plex); S13(Phospho); K17(TMT6plex) 2 Q9H1E3 Q9H1E3; Q9H1E3-2 1 4 0.0424 0 1 1 693.82727 2772.28726 2772.28728 -0.01 -1e-05 619620.125 CID (Collision Induced Dissociation) MS2 56.28654 40 191.8 60.0 66.2767 20076 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 196.4 212.8 161.6 150.8 176.7 209.0 218.3 208.7 193.9 189.8 Excluded by Method 2.83 1 0.0031 0.02228 False High Sequest HT (A2) Unambiguous LGDVSPTQIDVSQFGSFK [K].lGDVsPTQIDVSQFGSFk.[E] N-Term(TMT6plex); S5(Phospho); K18(TMT6plex) 1 P46821 P46821 0 3 0.0208 0 1 1 821.75797 2463.25936 2463.25728 0.84 0.00069 178594.640625 CID (Collision Induced Dissociation) MS2 55.74667 70 9.2 60.0 142.3549 48612 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 8.6 8.4 8.2 6.5 9.4 11.2 9.1 9.4 10.3 10.7 1.92 1 0.0031 0.02198 False High Sequest HT (A2) Unambiguous EEEAKEESDDDMGFGLFD [K].eEEAkEEsDDDmGFGLFD.[-] N-Term(TMT6plex); K5(TMT6plex); S8(Phospho); M12(Oxidation) 1 P02400 P02400 1 3 0.5397 0 1 1 873.03687 2617.09606 2617.0941 0.75 0.00065 652238.625 CID (Collision Induced Dissociation) MS2 25.77657 30 89.2 60.0 113.3518 37667 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 19.1 70.8 122.2 33.2 75.4 167.9 28.6 80.3 193.2 100.9 Excluded by Method 2.39 1 0.0031 0.02344 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVSsDEEF.[-] N-Term(TMT6plex); S9(Phospho) 1 P16521 P16521 0 2 0.0714 0 1 1 885.37095 1769.73463 1769.73562 -0.56 -0.0005 727172.75 CID (Collision Induced Dissociation) MS2 32.45543 40 82.9 60.0 118.8335 39684 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 36.7 70.3 115.9 36.1 70.3 151.6 43.5 81.7 146.7 75.7 Excluded by Method 2.8 1 0.0031 0.02425 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVssDEEF.[-] N-Term(TMT6plex); S8(Phospho); S9(Phospho) 1 P16521 P16521 0 2 0.2176 0 1 1 925.35602 1849.70476 1849.70195 1.52 0.0014 608203.125 CID (Collision Induced Dissociation) MS2 0.0 50 50.4 60.0 142.6654 48678 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 14.0 41.9 80.1 15.3 34.1 97.4 19.2 49.4 103.5 49.0 Excluded by Method 2.39 1 0.0031 0.02244 False High Sequest HT (A2) Unambiguous EVSPALK [K].eVsPALk.[A] N-Term(TMT6plex); S3(Phospho); K7(TMT6plex) 1 P36146 P36146 0 2 0.2395 0 1 1 641.36493 1281.72258 1281.72198 0.47 0.0003 1806434.25 CID (Collision Induced Dissociation) MS2 6.366906 30 109.4 60.0 67.6601 20534 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 30.2 104.3 145.7 32.5 85.8 211.0 44.4 104.3 227.5 108.6 Excluded by Method 2.63 1 0.0031 0.0225 False High Sequest HT (A2) Unambiguous NIILEEGK [K].nIILEEGk.[E] N-Term(TMT6plex); K8(TMT6plex) 1 P23528 P23528 0 2 0.1674 0 1 1 687.42376 1373.84025 1373.84044 -0.14 -0.0001 2335610.25 CID (Collision Induced Dissociation) MS2 0.0 60 41.3 60.0 80.165 25256 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 43.3 48.6 32.7 39.0 33.2 44.0 43.2 38.0 45.9 45.4 Excluded by Method 2.27 1 0.0031 0.0227 False High Sequest HT (A2) Unambiguous EEEAKEESDDDMGFGLFD [K].eEEAkEEsDDDmGFGLFD.[-] N-Term(TMT6plex); K5(TMT6plex); S8(Phospho); M12(Oxidation) 1 P02400 P02400 1 3 0.6044 0 1 1 873.03655 2617.0951 2617.0941 0.38 0.00033 1127372.25 CID (Collision Induced Dissociation) MS2 7.034232 40 104.1 60.0 122.9608 41230 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 23.9 86.9 148.0 31.7 89.1 179.7 39.1 96.8 233.9 111.6 Excluded by Method 2.73 1 0.0031 0.02227 False High Sequest HT (A2) Unambiguous NEDLGSLK [K].nEDLGsLk.[E] N-Term(TMT6plex); S6(Phospho); K8(TMT6plex) 1 Q06315 Q06315 0 2 0.2689 0 1 1 707.37299 1413.73869 1413.73908 -0.27 -0.00019 6514183.0 CID (Collision Induced Dissociation) MS2 0.0 20 126.3 48.76 74.6161 23136 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 92.0 110.4 127.1 107.9 114.3 162.0 104.4 126.2 189.7 129.2 Excluded by Method 2.64 1 0.0031 0.02393 False High Sequest HT (A2) Unambiguous IGEGTYGVVYK [K].iGEGtyGVVYk.[AG] N-Term(TMT6plex); T5(Phospho); Y6(Phospho); K11(TMT6plex) 5 P24941 P24941; P06493; Q00526; P24941-2; P06493-2 0 3 0.141 0 1 1 601.96269 1803.87353 1803.87354 -0.01 0.0 451031.28125 CID (Collision Induced Dissociation) MS2 12.93859 50 66.3 60.0 97.2006 31654 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 44.1 74.9 62.9 48.8 59.2 75.9 65.5 75.7 96.9 58.7 Excluded by Method 2.27 1 0.003237 0.0249 False High Sequest HT (A2) Unambiguous ENTPEQDDVATK [K].eNtPEQDDVATk.[K] N-Term(TMT6plex); T3(Phospho); K12(TMT6plex) 1 P06786 P06786 0 3 0.0063 0 1 1 628.97084 1884.89796 1884.89922 -0.67 -0.00042 658939.25 CID (Collision Induced Dissociation) MS2 45.56681 30 19.5 60.0 52.162 14789 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 9.2 15.2 26.5 9.6 18.7 31.3 10.5 15.6 37.6 21.1 Excluded by Method 1.6 1 0.003237 0.02496 False High Sequest HT (A2) Unambiguous DISTPDLSK [K].dIsTPDLSk.[V] N-Term(TMT6plex); S3(Phospho); K9(TMT6plex) 1 Q12675 Q12675 0 3 0.0325 0 1 1 505.26882 1513.79189 1513.79151 0.25 0.00013 455679.96875 CID (Collision Induced Dissociation) MS2 8.687666 30 8.8 60.0 78.6354 24735 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 3.6 8.7 9.0 5.9 7.1 17.7 3.2 10.5 10.9 11.8 Excluded by Method 1.54 1 0.003237 0.02441 False High Sequest HT (A2) Unambiguous YKLDEDEDEDDADLSK [K].ykLDEDEDEDDADLsk.[Y] N-Term(TMT6plex); K2(TMT6plex); S15(Phospho); K16(TMT6plex) 2 O95218 O95218-2; O95218 1 3 0.2882 0 1 1 889.75595 2667.25329 2667.25293 0.14 0.00012 4877744.0 CID (Collision Induced Dissociation) MS2 0.0 30 78.6 23.294 80.1539 25252 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 74.0 75.1 49.8 95.0 84.3 66.1 100.0 81.4 81.7 78.6 Excluded by Method 2.88 1 0.003237 0.02472 False High Sequest HT (A2) Unambiguous RLLGDSDSEEEQK [K].rLLGDsDsEEEQk.[E] N-Term(TMT6plex); S6(Phospho); S8(Phospho); K13(TMT6plex) 1 Q9BW71 Q9BW71 1 3 0.3523 0 1 1 708.66118 2123.96898 2123.96633 1.25 0.00088 678224.4375 CID (Collision Induced Dissociation) MS2 15.48651 30 58.8 60.0 77.6052 24349 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 54.7 52.7 51.1 59.8 53.1 64.8 67.7 58.8 66.9 58.1 Excluded by Method 2.81 1 0.003237 0.02471 False High Sequest HT (A2) Unambiguous SKPIPIMPASPQK [K].skPIPIMPAsPQk.[G] N-Term(TMT6plex); K2(TMT6plex); S10(Phospho); K13(TMT6plex) 9 O00429 O00429-4; O00429-9; O00429; O00429-6; O00429-8; O00429-7; O00429-3; O00429-5; O00429-2 0 4 0.5463 0 1 1 541.06593 2161.24187 2161.24231 -0.2 -0.00011 1272159.125 CID (Collision Induced Dissociation) MS2 5.675736 0 191.6 60.0 89.1937 28641 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 189.1 189.7 142.7 202.6 179.0 187.0 231.6 201.8 194.3 198.0 Excluded by Method 3.24 1 0.00335 0.02524 False High Sequest HT (A2) Unambiguous YAAAAPAATSAASGDAAPAEEAAAEEEEESDDDMGFGLFD [K].yAAAAPAATSAASGDAAPAEEAAAEEEEEsDDDMGFGLFD.[-] N-Term(TMT6plex); S30(Phospho) 1 P05317 P05317 0 4 0.2322 0 1 1 1058.19217 4229.74686 4229.73795 2.11 0.00223 210999.03125 CID (Collision Induced Dissociation) MS2 0.0 60 7.0 60.0 142.0367 48502 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 1.8 2.9 11.5 1.4 3.3 14.1 1.8 8.9 15.4 8.8 Excluded by Method 2.11 1 0.00335 0.02609 False High Sequest HT (A2) Unambiguous WFFISGSV [K].wFFISGsV.[-] N-Term(TMT6plex); S7(Phospho) 1 P00817 P00817 0 2 0.2022 0 1 1 626.30459 1251.6019 1251.60124 0.53 0.00033 295441.53125 CID (Collision Induced Dissociation) MS2 19.75673 60 30.6 60.0 136.9459 46720 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 12.0 25.1 45.1 9.7 18.6 59.4 13.2 29.9 61.4 31.2 Excluded by Method 1.78 1 0.00335 0.0253 False High Sequest HT (A2) Unambiguous KNPDEDEFLINSDDEM [K].kNPDEDEFLINsDDEm.[-] N-Term(TMT6plex); K1(TMT6plex); S12(Phospho); M16(Oxidation) 1 Q08412 Q08412 1 2 0.5485 0 1 1 1233.04473 2465.08219 2465.08314 -0.38 -0.00047 651269.8125 CID (Collision Induced Dissociation) MS2 0.0 20 36.9 60.0 92.4533 29873 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 7.5 30.6 55.4 12.6 32.5 66.9 13.7 34.4 70.7 44.4 Excluded by Method 2.06 1 0.00335 0.02613 False High Sequest HT (A2) Unambiguous NLENDTTSSPTQDLDEK [K].nLENDTTSsPTQDLDEk.[S] N-Term(TMT6plex); S9(Phospho); K17(TMT6plex) 1 Q04279 Q04279 0 3 0.0633 0 1 1 815.71929 2445.1433 2445.14343 -0.05 -4e-05 1913678.625 CID (Collision Induced Dissociation) MS2 3.74627 50 19.3 39.576 79.3483 24921 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 8.0 22.2 26.8 9.0 13.6 25.2 12.5 16.5 36.2 22.8 Excluded by Method 2.21 1 0.00335 0.02658 False High Sequest HT (A2) Unambiguous SPTASRISLGK [K].sPTASRISLGk.[K] N-Term(TMT6plex); S1(Phospho); K11(TMT6plex) 1 O94885 O94885 1 3 0.0691 0 1 1 552.31467 1654.92947 1654.92934 0.07 4e-05 1464333.625 CID (Collision Induced Dissociation) MS2 0.0 0 64.6 60.0 67.3636 20424 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 65.7 67.4 49.3 73.1 61.5 59.2 72.0 73.2 68.6 56.3 Excluded by Method 1.88 1 0.00335 0.02666 False High Sequest HT (A2) Unambiguous RCPTPEIQK [K].rcPtPEIQk.[K] N-Term(TMT6plex); C2(Carbamidomethyl); T4(Phospho); K9(TMT6plex) 2 P40818 P40818; P40818-2 1 3 0.2078 0 1 1 556.29678 1666.87578 1666.8752 0.35 0.00019 2995001.75 CID (Collision Induced Dissociation) MS2 3.85591 40 132.8 54.454 53.4228 15281 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 137.5 149.8 109.9 135.5 123.5 126.8 157.5 133.7 129.0 124.9 Excluded by Method 1.54 1 0.00335 0.0257 False High Sequest HT (A2) Unambiguous MEGSDFESSGGRGLK [K].mEGsDFESSGGRGLk.[K] N-Term(TMT6plex); M1(Oxidation); S4(Phospho); K15(TMT6plex) 2 Q8WYH8 Q8WYH8-2; Q8WYH8 1 3 0.2788 0 1 1 704.33451 2110.98896 2110.98806 0.43 0.0003 2324105.75 CID (Collision Induced Dissociation) MS2 0.0 10 121.9 60.0 60.9959 18068 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 126.0 126.9 99.9 114.9 121.0 121.0 129.3 133.7 120.5 126.2 Excluded by Method 2.08 1 0.00335 0.02581 False High Sequest HT (A2) Unambiguous ELEAERSLMSSPEDLTK [K].eLEAERSLmssPEDLTk.[D] N-Term(TMT6plex); M9(Oxidation); S10(Phospho); S11(Phospho); K17(TMT6plex) 1 P46821 P46821 1 4 0.0812 0 1 1 643.05328 2569.19128 2569.19099 0.11 7e-05 473272.75 CID (Collision Induced Dissociation) MS2 47.92283 40 39.2 60.0 89.8122 28874 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 41.9 40.2 32.9 40.1 34.5 36.2 41.1 42.1 40.3 42.7 Excluded by Method 3.45 1 0.00335 0.02747 False High Sequest HT (A2) Unambiguous KTSTSPPPEK [K].ktSTSPPPEk.[S] N-Term(TMT6plex); K1(TMT6plex); T2(Phospho); K10(TMT6plex) 2 Q9H1E3 Q9H1E3; Q9H1E3-2 1 4 0.0037 0 1 1 460.51129 1839.02335 1839.02319 0.09 4e-05 498804.8125 CID (Collision Induced Dissociation) MS2 7.680379 40 213.4 60.0 46.0432 12550 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 223.7 213.2 162.4 226.7 207.6 192.9 248.6 236.9 191.7 230.6 Excluded by Method 2.7 1 0.00335 0.02557 False High Sequest HT (A2) Unambiguous FGLSPSK [K].fGLsPSk.[G] N-Term(TMT6plex); S4(Phospho); K7(TMT6plex) 1 P25694 P25694 0 3 0.2817 0 1 1 425.23695 1273.69629 1273.69576 0.42 0.00018 311424.5625 CID (Collision Induced Dissociation) MS2 25.53083 50 178.4 60.0 87.0898 27911 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 36.2 149.2 284.1 58.4 141.4 331.3 66.5 167.5 376.3 173.1 Excluded by Method 1.42 1 0.00335 0.02707 False High Sequest HT (A2) Unambiguous GTVQQADE [K].gTVQQADE.[-] N-Term(TMT6plex) 1 P32969 P32969 0 2 0.4676 0 1 1 538.77497 1076.54267 1076.54213 0.5 0.00027 233570.046875 CID (Collision Induced Dissociation) MS2 0.0 70 20.1 60.0 29.39 6600 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 18.9 24.0 17.4 19.5 18.0 22.6 20.2 19.6 22.9 17.7 1.39 1 0.00335 0.02504 False High Sequest HT (A2) Unambiguous SRSNLSQENDNEGK [K].sRSNLSQENDNEGk.[H] N-Term(TMT6plex); S1(Phospho); K14(TMT6plex) 1 P39705 P39705 1 3 0.0503 0 1 1 706.00713 2116.00683 2116.00721 -0.18 -0.00013 212869.125 CID (Collision Induced Dissociation) MS2 12.5778 30 17.8 60.0 38.6182 9869 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 3.5 11.6 27.6 6.6 16.5 33.7 7.4 18.1 35.4 17.3 Excluded by Method 1.59 1 0.00335 0.02535 False High Sequest HT (A2) Unambiguous GEENGEVINTEEEEEEEHQQK [K].gEENGEVINtEEEEEEEHQQk.[G] N-Term(TMT6plex); T10(Phospho); K21(TMT6plex) 1 P38996 P38996 0 3 0.5263 0 1 1 1008.7844 3024.33864 3024.33593 0.89 0.0009 512332.375 CID (Collision Induced Dissociation) MS2 0.0 70 6.3 60.0 61.676 18356 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 1.3 10.9 7.3 1.5 3.1 8.8 2.9 8.1 11.9 7.2 2.28 1 0.00335 0.0271 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVSsDEEF.[-] N-Term(TMT6plex); S9(Phospho) 1 P16521 P16521 0 2 0.0047 0 1 1 885.3721 1769.73693 1769.73562 0.74 0.00065 301462.125 CID (Collision Induced Dissociation) MS2 0.0 50 35.9 60.0 146.5435 50023 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 9.0 26.2 56.3 10.2 30.5 73.6 13.5 28.3 71.4 40.3 Excluded by Method 2.11 1 0.00335 0.02721 False High Sequest HT (A2) Unambiguous DRRGSNLQSHEQK [K].dRRGSNLQsHEQk.[F] N-Term(TMT6plex); S9(Phospho); K13(TMT6plex) 1 P16965 P16965 2 4 0.0354 0 1 1 524.02179 2093.06532 2093.06534 -0.01 0.0 99221.3046875 CID (Collision Induced Dissociation) MS2 17.73674 30 8.8 60.0 25.0085 5155 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 2.0 11.5 10.0 3.1 9.4 13.5 3.6 9.4 12.4 13.0 Excluded by Method 1.98 1 0.00335 0.0274 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVssDEEF.[-] N-Term(TMT6plex); S8(Phospho); S9(Phospho) 1 P16521 P16521 0 2 0.1462 0 1 1 925.35535 1849.70342 1849.70195 0.79 0.00073 436175.625 CID (Collision Induced Dissociation) MS2 23.65702 30 61.0 60.0 145.9478 49804 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 17.4 50.6 91.9 19.6 50.2 108.6 16.1 61.4 126.4 67.3 Excluded by Method 2.53 1 0.00335 0.02504 False High Sequest HT (A2) Unambiguous LIRDGSIDLVINLPNNNTK [K].lIRDGSIDLVINLPNNNTk.[F] N-Term(TMT6plex); K19(TMT6plex) 3 P31327 P31327; P31327-3; P31327-2 1 3 0.1146 0 1 1 856.50144 2567.48976 2567.49211 -0.91 -0.00078 968063.0625 CID (Collision Induced Dissociation) MS2 51.22374 80 31.5 60.0 119.5407 39946 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 29.2 27.2 30.6 27.7 26.0 32.0 35.0 31.8 41.9 33.5 1.92 1 0.00335 0.02555 False High Sequest HT (A2) Unambiguous EEEEAKEESDDDMGFGLFD [K].eEEEAkEEsDDDmGFGLFD.[-] N-Term(TMT6plex); K6(TMT6plex); S9(Phospho); M13(Oxidation) 1 P05318 P05318 1 3 0.5157 0 1 1 916.05004 2746.13557 2746.13669 -0.41 -0.00037 723736.875 CID (Collision Induced Dissociation) MS2 24.93642 40 106.5 42.614 118.0092 39414 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 24.6 86.2 180.3 40.9 95.6 189.5 36.9 89.9 211.3 109.5 Excluded by Method 3.18 1 0.00335 0.02727 False High Sequest HT (A2) Unambiguous RTCPSLSPTSPLNNK [K].rTcPsLsPTSPLNNk.[G] N-Term(TMT6plex); C3(Carbamidomethyl); S5(Phospho); S7(Phospho); K15(TMT6plex) 2 O75925 O75925; O75925-2 1 3 0.2774 0 1 1 764.0411 2290.10873 2290.10681 0.84 0.00064 334381.71875 CID (Collision Induced Dissociation) MS2 71.67341 30 72.2 60.0 79.4477 24958 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 74.9 72.3 60.5 69.0 69.5 69.4 79.9 88.0 71.1 67.7 Excluded by Method 2.92 1 0.00346 0.02959 False High Sequest HT (A2) Unambiguous SPSLSPSPPSPLEK [K].sPSLSPsPPsPLEk.[T] N-Term(TMT6plex); S7(Phospho); S10(Phospho); K14(TMT6plex) 1 P46821 P46821 0 3 0.2753 0 1 1 681.00672 2041.0056 2041.00601 -0.2 -0.00014 3438177.75 CID (Collision Induced Dissociation) MS2 5.944226 40 117.3 35.92 94.8216 30762 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 118.3 113.0 100.2 114.6 116.6 124.7 132.3 112.7 118.4 122.5 Excluded by Method 2.47 1 0.00346 0.02962 False High Sequest HT (A2) Unambiguous EEEAKEESDDDMGFGLFD [K].eEEAkEEsDDDmGFGLFD.[-] N-Term(TMT6plex); K5(TMT6plex); S8(Phospho); M12(Oxidation) 1 P02400 P02400 1 3 0.6546 0 1 1 873.03608 2617.09368 2617.0941 -0.16 -0.00014 1009537.75 CID (Collision Induced Dissociation) MS2 6.926423 20 79.2 60.0 123.535 41411 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 23.9 67.1 111.5 30.1 69.2 143.3 29.3 75.8 167.0 74.8 Excluded by Method 2.49 1 0.00346 0.02842 False High Sequest HT (A2) Unambiguous ELESPLTPGK [K].eLESPLtPGk.[V] N-Term(TMT6plex); T7(Phospho); K10(TMT6plex) 1 O15014 O15014 0 3 0.3641 0 1 1 536.95994 1608.86528 1608.86501 0.17 9e-05 3059696.75 CID (Collision Induced Dissociation) MS2 21.99522 40 45.9 38.483 84.3628 26897 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 36.2 39.7 43.5 38.3 45.0 59.7 49.4 45.1 48.7 53.6 Excluded by Method 3.57 1 0.00346 0.02955 False High Sequest HT (A2) Unambiguous RIVAPPGGRSNITSLS [K].rIVAPPGGRSNITSLs.[-] N-Term(TMT6plex); S16(Phospho) 2 Q14195-2 Q14195-2; Q14195 2 3 0.1818 0 1 1 645.35235 1934.04249 1934.04219 0.15 0.0001 676196.25 CID (Collision Induced Dissociation) MS2 19.54082 60 57.3 60.0 72.3223 22361 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 41.5 100.2 49.1 53.3 53.3 60.4 53.5 50.4 53.5 58.0 Excluded by Method 2.2 1 0.00346 0.0297 False High Sequest HT (A2) Unambiguous SIDDEDVDENEDDVYGNSSGRK [K].sIDDEDVDENEDDVYGNSSGRk.[H] N-Term(TMT6plex); S1(Phospho); K22(TMT6plex) 3 Q8TEQ0 Q8TEQ0; Q8IUI4; Q8TEQ0-2 1 3 0.1064 0 1 1 999.43877 2996.30174 2996.30463 -0.96 -0.00096 257216.59375 CID (Collision Induced Dissociation) MS2 24.54167 60 4.8 60.0 71.1168 21906 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 3.1 6.8 3.4 6.8 7.2 2.9 3.8 7.7 3.8 2.5 Excluded by Method 2.35 1 0.00346 0.02818 False High Sequest HT (A2) Unambiguous ISSSEDFSGK [K].iSsSEDFSGk.[D] N-Term(TMT6plex); S3(Phospho); K10(TMT6plex) 1 P38065 P38065 0 2 0.1246 0 1 1 797.892 1594.77672 1594.77659 0.08 6e-05 919056.125 CID (Collision Induced Dissociation) MS2 0.0 40 71.0 60.0 75.7277 23585 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 15.6 52.7 112.6 27.1 66.8 137.2 24.5 66.0 137.7 70.2 Excluded by Method 2.89 1 0.00346 0.02846 False High Sequest HT (A2) Unambiguous DESDSENDIEIK [K].dESDsENDIEIk.[G] N-Term(TMT6plex); S5(Phospho); K12(TMT6plex) 1 P32499 P32499 0 3 0.0745 0 1 1 644.63415 1931.88789 1931.88872 -0.43 -0.00028 352912.3125 CID (Collision Induced Dissociation) MS2 24.94667 50 16.4 60.0 76.048 23677 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 11.7 14.6 18.9 15.8 13.2 20.6 13.7 17.2 21.9 16.6 Excluded by Method 1.88 1 0.00346 0.02867 False High Sequest HT (A2) Unambiguous LALDMEISAYRK [K].lALDmEIsAYRk.[L] N-Term(TMT6plex); M5(Oxidation); S8(Phospho); K12(TMT6plex) 1 P20700 P20700 1 3 0.1356 0 1 1 655.34922 1964.03309 1964.03282 0.14 9e-05 2002842.625 CID (Collision Induced Dissociation) MS2 16.33178 20 126.1 60.0 97.5038 31740 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 123.8 126.9 105.4 126.0 126.1 128.0 127.6 143.5 130.8 122.7 Excluded by Method 1.77 1 0.00346 0.02981 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVSsDEEF.[-] N-Term(TMT6plex); S9(Phospho) 1 P16521 P16521 0 2 0.0338 0 1 1 885.37138 1769.73548 1769.73562 -0.08 -7e-05 182274.828125 CID (Collision Induced Dissociation) MS2 0.0 40 25.7 60.0 143.0079 48799 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 8.5 19.3 36.3 9.5 27.1 53.6 7.0 21.1 48.0 26.3 Excluded by Method 1.48 1 0.00346 0.02826 False High Sequest HT (A2) Unambiguous IMATPEQVGK [K].imAtPEQVGk.[M] N-Term(TMT6plex); M2(Oxidation); T4(Phospho); K10(TMT6plex) 2 P12956 P12956; P12956-2 0 2 1.0 0 1 1 814.4289 1627.85053 1627.85307 -1.56 -0.00127 740736.3125 CID (Collision Induced Dissociation) MS2 21.84646 10 148.6 60.0 68.5054 20862 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 145.3 145.9 125.0 141.4 142.3 158.4 178.8 146.5 151.2 151.7 Excluded by Method 2.79 1 0.00346 0.02816 False High Sequest HT (A2) Unambiguous IGEGTYGVVYK [K].iGEGtyGVVYk.[AG] N-Term(TMT6plex); T5(Phospho); Y6(Phospho); K11(TMT6plex) 5 P24941 P24941; P06493; Q00526; P24941-2; P06493-2 0 2 0.4079 0 1 1 902.44125 1803.87522 1803.87354 0.93 0.00084 925117.25 CID (Collision Induced Dissociation) MS2 22.55223 30 53.5 60.0 97.482 31817 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 41.1 56.1 44.6 52.1 55.5 55.8 63.2 56.0 58.0 53.0 Excluded by Method 2.77 1 0.00346 0.02883 False High Sequest HT (A2) Unambiguous SSEASPTAQRSK [K].sSEAsPTAQRSk.[D] N-Term(TMT6plex); S5(Phospho); K12(TMT6plex) 1 Q7Z569 Q7Z569 1 3 0.2444 0 1 1 596.30867 1786.91147 1786.91006 0.78 0.00047 129230.3515625 CID (Collision Induced Dissociation) MS2 0.0 20 30.5 60.0 30.9545 7092 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 32.7 30.8 27.7 33.9 29.7 27.7 28.8 31.8 33.1 28.7 Excluded by Method 1.8 1 0.00346 0.02856 False High Sequest HT (A2) Unambiguous DDSHSAEDSEDEK [K].dDSHsAEDsEDEk.[E] N-Term(TMT6plex); S5(Phospho); S9(Phospho); K13(TMT6plex) 2 Q9H1E3 Q9H1E3; Q9H1E3-2 0 3 0.4554 0 1 1 694.60598 2081.80339 2081.79899 2.11 0.00146 200491.78125 CID (Collision Induced Dissociation) MS2 3.428412 30 27.2 60.0 35.3365 8640 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 31.1 24.7 29.1 25.6 23.7 28.5 23.4 31.8 26.8 27.0 Excluded by Method 3.03 1 0.00346 0.02959 False High Sequest HT (A2) Unambiguous SHHANSPTAGAAK [K].sHHANSPTAGAAk.[S] N-Term(TMT6plex); S1(Phospho); K13(TMT6plex) 5 Q15555 Q15555-2; Q15555-3; Q15555-5; Q15555-4; Q15555 0 3 0.0158 0 1 1 596.30497 1786.90037 1786.90017 0.11 7e-05 40454.109375 CID (Collision Induced Dissociation) MS2 0.0 80 4.7 60.0 22.5769 4442 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 3.3 3.2 2.7 3.9 6.5 3.2 6.5 3.8 6.2 8.2 1.9 1 0.00346 0.02803 False High Sequest HT (A2) Unambiguous TLDFDALSVGQRGAK [K].tLDFDALsVGQRGAk.[T] N-Term(TMT6plex); S8(Phospho); K15(TMT6plex) 1 Q14195-2 Q14195-2 1 3 0.3463 0 1 1 706.04479 2116.11981 2116.12039 -0.27 -0.00019 1042826.625 CID (Collision Induced Dissociation) MS2 7.356939 20 38.3 60.0 102.8851 33837 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 23.7 40.1 39.9 18.3 35.6 59.9 24.9 36.5 65.4 39.0 Excluded by Method 2.57 1 0.00346 0.02929 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVsSDEEF.[-] N-Term(TMT6plex); S8(Phospho) 1 P16521 P16521 0 2 0.0537 0 1 1 885.37169 1769.73609 1769.73562 0.27 0.00024 188769.421875 CID (Collision Induced Dissociation) MS2 31.61473 50 30.8 60.0 145.199 49595 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 10.4 27.7 50.4 9.4 28.2 58.6 9.8 27.3 59.4 27.0 Excluded by Method 2.05 1 0.00346 0.02858 False High Sequest HT (A2) Unambiguous ELEAERSLMSSPEDLTK [K].eLEAERSLMssPEDLTk.[D] N-Term(TMT6plex); S10(Phospho); S11(Phospho); K17(TMT6plex) 1 P46821 P46821 1 3 0.4215 0 1 1 851.73622 2553.19412 2553.19607 -0.77 -0.00065 992945.0625 CID (Collision Induced Dissociation) MS2 0.0 30 102.3 60.0 108.0118 35710 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 105.8 109.5 75.1 120.9 85.9 95.2 119.2 112.9 101.5 97.3 Excluded by Method 3.25 1 0.00346 0.02923 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVSsDEEF.[-] N-Term(TMT6plex); S9(Phospho) 1 P16521 P16521 0 2 0.0345 0 1 1 885.37149 1769.73571 1769.73562 0.05 4e-05 235198.390625 CID (Collision Induced Dissociation) MS2 34.5798 50 26.8 60.0 140.9116 48075 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 6.4 21.5 42.1 6.4 22.9 53.2 9.6 20.3 56.4 29.0 Excluded by Method 2.32 1 0.00346 0.02942 False High Sequest HT (A2) Unambiguous EEEAAEESDDDMGFGLFD [K].eEEAAEEsDDDmGFGLFD.[-] N-Term(TMT6plex); S8(Phospho); M12(Oxidation) 2 P05319 P10622; P05319 0 3 0.6136 0 1 1 777.63214 2330.88186 2330.87332 3.66 0.00285 1513491.75 CID (Collision Induced Dissociation) MS2 1.801981 40 93.4 47.767 144.8084 49422 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 21.6 75.2 142.4 29.3 84.1 178.8 32.8 95.6 187.0 87.1 Excluded by Method 2.2 1 0.00346 0.02903 False High Sequest HT (A2) Unambiguous LYERRGGGSGGGEESEGEEVDED [K].lYERRGGGSGGGEEsEGEEVDED.[-] N-Term(TMT6plex); S15(Phospho) 1 Q96QR8 Q96QR8 2 3 0.031 0 1 1 908.04879 2722.13181 2722.13147 0.12 0.00011 407679.03125 CID (Collision Induced Dissociation) MS2 0.0 70 13.4 60.0 54.8624 15811 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 14.7 14.6 11.3 13.3 10.1 12.1 12.5 14.4 14.6 16.4 2.9 1 0.00346 0.02966 False High Sequest HT (A2) Unambiguous KEESEESDDDMGFGLFD [K].kEESEEsDDDmGFGLFD.[-] N-Term(TMT6plex); K1(TMT6plex); S7(Phospho); M11(Oxidation) 3 P05387 P05386; P05386-2; P05387 1 2 0.0137 0 1 1 1252.52617 2504.04507 2504.04642 -0.54 -0.00068 669638.125 CID (Collision Induced Dissociation) MS2 0.0 20 46.3 60.0 114.4971 38089 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 50.4 47.0 31.1 47.1 35.7 45.0 54.1 48.3 58.1 46.7 Excluded by Method 2.19 1 0.003575 0.03185 False High Sequest HT (A2) Unambiguous NISSNRPADTRDIVADKPILGFNAFAALESEDEDDEA [K].nISSNRPADTRDIVADkPILGFNAFAALEsEDEDDEA.[-] N-Term(TMT6plex); K17(TMT6plex); S30(Phospho) 1 P12962 P12962 1 5 0.4345 0 1 1 909.64276 4544.1847 4544.18703 -0.51 -0.00047 9595213.0 CID (Collision Induced Dissociation) MS2 10.32008 60 24.9 4.933 128.2603 43257 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 8.3 23.5 37.9 9.2 19.6 44.6 8.0 22.4 51.3 24.2 Excluded by Method 2.9 1 0.003575 0.03047 False High Sequest HT (A2) Unambiguous SHERPDDVSV [K].sHERPDDVsV.[-] N-Term(TMT6plex); S9(Phospho) 1 P54003 P54003 0 3 0.6265 0 1 1 483.89066 1449.65744 1449.65725 0.13 6e-05 1871461.75 CID (Collision Induced Dissociation) MS2 2.151756 10 176.6 60.0 39.9839 10361 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 42.3 138.1 284.0 50.1 137.8 352.3 58.8 161.7 360.2 180.4 Excluded by Method 3.32 1 0.003575 0.03081 False High Sequest HT (A2) Unambiguous KVELNIVLPELRPVETSPTK [K].kVELNIVLPELRPVEtSPTk.[Q] N-Term(TMT6plex); K1(TMT6plex); T16(Phospho); K20(TMT6plex) 1 Q06604 Q06604 1 4 0.009 0 1 1 758.19647 3029.76404 3029.76189 0.71 0.00054 402957.46875 CID (Collision Induced Dissociation) MS2 26.09966 60 78.4 60.0 123.9972 41662 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 23.0 63.7 115.2 21.9 55.1 148.2 34.0 79.7 169.8 73.7 Excluded by Method 2.21 1 0.003575 0.03032 False High Sequest HT (A2) Unambiguous EEPLSEEEPCTSTAIASPEKK [K].eEPLsEEEPcTsTAIASPEkk.[K] N-Term(TMT6plex); S5(Phospho); C10(Carbamidomethyl); S12(Phospho); K20(TMT6plex); K21(TMT6plex) 1 Q9Y2X3 Q9Y2X3 1 3 0.2406 0 1 1 1060.50662 3179.50531 3179.50752 -0.69 -0.00074 1128951.75 CID (Collision Induced Dissociation) MS2 0.0 30 112.1 60.0 86.5971 27645 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 123.2 108.3 81.9 140.2 105.0 98.1 134.4 110.9 107.9 111.0 Excluded by Method 3.99 1 0.003575 0.03091 False High Sequest HT (A2) Unambiguous SPTASRISLGK [K].sPTASRISLGk.[K] N-Term(TMT6plex); S1(Phospho); K11(TMT6plex) 1 O94885 O94885 1 3 0.0603 0 1 1 552.31487 1654.93007 1654.92934 0.44 0.00024 1252450.625 CID (Collision Induced Dissociation) MS2 6.844601 10 48.6 60.0 66.2944 20040 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 57.7 47.9 35.4 54.0 43.9 47.2 50.4 52.0 48.2 48.9 Excluded by Method 1.99 1 0.003575 0.03005 False High Sequest HT (A2) Unambiguous SPGASNFSTLPK [K].sPGASNFSTLPk.[I] N-Term(TMT6plex); S1(Phospho); K12(TMT6plex) 1 Q9Y4E8 Q9Y4E8 0 3 0.0435 0 1 1 581.97467 1743.90945 1743.90827 0.67 0.00039 9134750.0 CID (Collision Induced Dissociation) MS2 7.955101 30 125.4 13.638 85.7251 27401 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 138.1 124.4 97.0 131.3 124.9 117.3 140.8 129.9 118.7 131.2 Excluded by Method 2.99 1 0.003575 0.03096 False High Sequest HT (A2) Unambiguous ELEAERSLMSSPEDLTK [K].eLEAERSLMssPEDLTk.[D] N-Term(TMT6plex); S10(Phospho); S11(Phospho); K17(TMT6plex) 1 P46821 P46821 1 3 0.3311 0 1 1 851.73793 2553.19924 2553.19607 1.24 0.00106 771730.6875 CID (Collision Induced Dissociation) MS2 20.57409 30 56.3 60.0 109.7578 36397 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 56.3 60.7 52.0 56.2 51.6 52.0 55.9 63.3 64.8 50.2 Excluded by Method 2.96 1 0.003575 0.03259 False High Sequest HT (A2) Unambiguous HLDVDLDRQSLSSIDK [K].hLDVDLDRQSLssIDk.[N] N-Term(TMT6plex); S12(Phospho); S13(Phospho); K16(TMT6plex) 1 Q9Y6D5 Q9Y6D5 1 3 0.043 0 1 1 820.40411 2459.19779 2459.19846 -0.27 -0.00022 717508.25 CID (Collision Induced Dissociation) MS2 0.0 40 93.0 60.0 93.907 30421 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 73.4 92.5 81.4 88.7 87.5 108.5 100.2 104.5 114.3 79.2 Excluded by Method 2.79 1 0.003575 0.03228 False High Sequest HT (A2) Unambiguous ELDTAQK [K].eLDTAQk.[H] N-Term(TMT6plex); K7(TMT6plex) 3 P00359 P00360; P00359; P00358 0 2 0.2669 0 1 1 631.87238 1262.73749 1262.73564 1.47 0.00093 756391.875 CID (Collision Induced Dissociation) MS2 29.99246 50 34.2 60.0 49.307 13742 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 19.0 26.3 46.4 16.4 27.3 53.6 29.6 32.5 53.4 37.8 Excluded by Method 2.36 1 0.003575 0.03188 False High Sequest HT (A2) Unambiguous LPWEVSDGEVSSGK [K].lPWEVsDGEVSSGk.[T] N-Term(TMT6plex); S6(Phospho); K14(TMT6plex) 1 P48415 P48415 0 3 0.3375 0 1 1 676.67391 2028.00717 2028.00911 -0.96 -0.00065 641851.6875 CID (Collision Induced Dissociation) MS2 35.72493 20 87.9 60.0 113.5169 37798 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 71.1 85.4 88.0 74.7 79.5 105.1 76.8 92.1 120.0 86.0 Excluded by Method 2.4 1 0.003575 0.03051 False High Sequest HT (A2) Unambiguous EEEAKEESDDDMGFGLFD [K].eEEAkEEsDDDmGFGLFD.[-] N-Term(TMT6plex); K5(TMT6plex); S8(Phospho); M12(Oxidation) 1 P02400 P02400 1 3 0.4919 0 1 1 873.03692 2617.09619 2617.0941 0.8 0.0007 1092757.0 CID (Collision Induced Dissociation) MS2 2.971869 30 109.3 60.0 124.0789 41642 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 29.3 95.8 149.5 38.0 95.8 195.9 39.6 103.2 240.7 105.3 Excluded by Method 2.46 1 0.003575 0.03021 False High Sequest HT (A2) Unambiguous AAADALSDLEIK [K].aAADALsDLEIk.[D] N-Term(TMT6plex); S7(Phospho); K12(TMT6plex) 1 P09938 P09938 0 3 0.0829 0 1 1 585.64967 1754.93445 1754.93415 0.17 0.0001 567397.3125 CID (Collision Induced Dissociation) MS2 0.0 30 32.1 60.0 115.0997 38346 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 9.0 24.4 44.5 16.9 23.1 60.5 10.9 30.8 62.6 37.9 Excluded by Method 2.17 1 0.003575 0.03008 False High Sequest HT (A2) Unambiguous DSTLIMQLLRDNLTLWTSENQGDEGDAGEGEN [K].dSTLImQLLRDNLTLWTSENQGDEGDAGEGEN.[-] N-Term(TMT6plex); M6(Oxidation) 2 P31946 P31946-2; P31946 1 4 0.3165 0 1 1 942.44786 3766.76961 3766.75512 3.85 0.00362 286072.90625 CID (Collision Induced Dissociation) MS2 10.33462 50 6.8 60.0 136.4475 46541 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 6.1 8.2 6.6 3.4 8.0 10.4 7.5 4.0 6.5 7.4 Excluded by Method 2.97 1 0.003575 0.03197 False High Sequest HT (A2) Unambiguous TELNSSAESEQPLDK [K].tELNSsAESEQPLDk.[T] N-Term(TMT6plex); S6(Phospho); K15(TMT6plex) 4 Q9Y5B6 Q9Y5B6-4; Q9Y5B6-3; Q9Y5B6; Q9Y5B6-2 0 3 0.0192 0 1 1 729.35769 2186.05852 2186.06299 -2.05 -0.00149 581693.9375 CID (Collision Induced Dissociation) MS2 40.70277 30 61.4 60.0 76.405 23897 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 63.1 60.9 55.6 57.4 57.0 66.1 63.4 61.9 65.4 62.9 Excluded by Method 2.61 1 0.003575 0.03024 False High Sequest HT (A2) Unambiguous VFDDESDEK [K].vFDDEsDEk.[E] N-Term(TMT6plex); S6(Phospho); K9(TMT6plex) 1 O43719 O43719 0 2 0.2848 0 1 1 811.373 1621.73872 1621.73987 -0.71 -0.00058 589727.75 CID (Collision Induced Dissociation) MS2 0.0 40 50.1 60.0 76.0362 23702 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 46.8 52.1 48.3 53.6 37.6 61.0 57.4 51.3 48.2 44.5 Excluded by Method 3.23 1 0.003575 0.03052 False High Sequest HT (A2) Unambiguous VLPELQGK [K].vLPELQGk.[L] N-Term(TMT6plex); K8(TMT6plex) 3 P00359 P00360; P00359; P00358 0 2 0.285 0 1 1 671.42935 1341.85141 1341.85061 0.6 0.0004 1049848.25 CID (Collision Induced Dissociation) MS2 43.65299 40 177.3 60.0 89.9048 28967 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 95.9 147.8 259.2 88.4 154.7 280.1 96.2 167.8 291.5 191.5 Excluded by Method 1.93 1 0.003716 0.03287 False High Sequest HT (A2) Unambiguous EQAEASIDNLK [K].eQAEAsIDNLk.[N] N-Term(TMT6plex); S6(Phospho); K11(TMT6plex) 1 Q08245 Q08245 0 3 0.1855 0 1 1 585.96909 1755.89272 1755.89302 -0.17 -0.0001 5138824.5 CID (Collision Induced Dissociation) MS2 9.7307 50 102.1 31.015 68.6597 20904 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 33.2 71.5 142.1 42.4 93.1 198.4 43.4 93.9 193.3 109.4 Excluded by Method 2.48 1 0.003845 0.03288 False High Sequest HT (A2) Unambiguous IRNSGDDTSMLFQDDESDQKVPWEEDVK [K].iRNSGDDTsMLFQDDEsDQkVPWEEDVk.[K] N-Term(TMT6plex); S9(Phospho); S17(Phospho); K20(TMT6plex); K28(TMT6plex) 1 P48415 P48415 2 4 0.0349 0 1 1 1033.47969 4130.89695 4130.89101 1.44 0.00148 1641292.125 CID (Collision Induced Dissociation) MS2 0.0 20 46.3 60.0 85.2169 27151 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 41.6 45.8 35.3 51.6 48.3 42.7 62.8 48.5 51.6 34.6 Excluded by Method 4.3 1 0.003845 0.03422 False High Sequest HT (A2) Unambiguous DDGSQSPIRK [K].dDGSQsPIRk.[Q] N-Term(TMT6plex); S6(Phospho); K10(TMT6plex) 1 Q02969 Q02969 1 3 0.2395 0 1 1 547.61858 1640.84117 1640.84092 0.15 8e-05 1929321.0 CID (Collision Induced Dissociation) MS2 11.95316 20 18.2 60.0 43.6437 11635 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 7.3 19.3 19.6 15.4 15.8 25.1 12.6 18.3 31.9 16.9 Excluded by Method 1.67 1 0.003845 0.03386 False High Sequest HT (A2) Unambiguous AAADALSDLEIK [K].aAADALsDLEIk.[D] N-Term(TMT6plex); S7(Phospho); K12(TMT6plex) 1 P09938 P09938 0 3 0.0548 0 1 1 585.64947 1754.93385 1754.93415 -0.17 -0.0001 942591.5625 CID (Collision Induced Dissociation) MS2 57.5667 30 77.6 60.0 114.4211 38136 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 25.6 71.4 112.7 25.3 58.8 146.7 25.6 72.7 154.9 82.4 Excluded by Method 2.92 1 0.003845 0.03297 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVsSDEEF.[-] N-Term(TMT6plex); S8(Phospho) 1 P16521 P16521 0 2 0.0098 0 1 1 885.37271 1769.73815 1769.73562 1.43 0.00126 195047.28125 CID (Collision Induced Dissociation) MS2 0.0 40 22.9 60.0 145.4233 49637 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 7.4 18.4 33.1 11.2 14.4 43.0 10.2 24.3 44.3 23.1 Excluded by Method 2.04 1 0.003845 0.03427 False High Sequest HT (A2) Unambiguous SSLFLPSGSSSTSLK [K].sSLFLPSGSsSTSLk.[F] N-Term(TMT6plex); S10(Phospho); K15(TMT6plex) 1 P48524 P48524 0 3 0.0253 0 1 1 679.36255 2036.07311 2036.07171 0.69 0.00047 1065650.75 CID (Collision Induced Dissociation) MS2 5.050586 50 100.4 60.0 112.2465 37291 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 25.0 82.5 159.2 32.5 88.5 184.0 37.3 100.0 202.4 92.5 Excluded by Method 2.77 1 0.003845 0.03413 False High Sequest HT (A2) Unambiguous RASFAK [K].rAsFAk.[S] N-Term(TMT6plex); S3(Phospho); K6(TMT6plex) 12 Q9H4A3 Q9H4A3-5; Q9Y3S1; Q9H4A3-2; Q9Y3S1-2; Q96J92; Q9H4A3-7; Q9H4A3; Q96J92-2; Q96J92-3; Q9Y3S1-4; Q9H4A3-6; Q9Y3S1-3 1 3 0.1972 0 1 1 406.56527 1217.68127 1217.68078 0.4 0.00016 1265971.25 CID (Collision Induced Dissociation) MS2 3.661903 30 289.8 60.0 48.2271 13309 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 283.7 279.3 243.1 297.7 273.7 287.6 323.3 316.7 280.0 313.0 Excluded by Method 2.89 1 0.003845 0.03344 False High Sequest HT (A2) Unambiguous LSLGQYDNDAGGQLPFSK [K].lSLGQyDNDAGGQLPFSk.[C] N-Term(TMT6plex); Y6(Phospho); K18(TMT6plex) 2 Q68CZ2 Q68CZ2; Q68CZ2-2 0 3 0.0229 0 1 1 816.74407 2448.21767 2448.22123 -1.45 -0.00119 365127.3125 CID (Collision Induced Dissociation) MS2 11.37363 40 57.0 60.0 118.8657 39780 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 59.8 59.4 49.4 61.5 50.5 56.0 62.6 57.5 60.9 52.4 Excluded by Method 2.18 1 0.003845 0.0341 False High Sequest HT (A2) Unambiguous VGEGTYGVVYK [K].vGEGtYGVVYk.[A] N-Term(TMT6plex); T5(Phospho); K11(TMT6plex) 1 P00546 P00546 0 3 0.0415 0 1 1 570.63516 1709.89093 1709.89156 -0.37 -0.00021 442922.3125 CID (Collision Induced Dissociation) MS2 0.0 50 41.4 60.0 81.4828 25752 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 23.3 36.6 47.7 29.9 31.3 62.2 24.4 42.2 70.3 45.7 Excluded by Method 2.17 1 0.003845 0.03346 False High Sequest HT (A2) Unambiguous SHERPDDVSV [K].sHERPDDVsV.[-] N-Term(TMT6plex); S1(Phospho); S9(Phospho) 1 P54003 P54003 0 2 0.5393 0 1 1 765.31593 1529.62459 1529.62359 0.66 0.0005 2750656.0 CID (Collision Induced Dissociation) MS2 24.15264 40 122.7 54.972 48.1672 13286 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 35.1 90.1 197.1 39.7 92.3 235.8 55.5 119.5 242.0 119.8 Excluded by Method 2.8 1 0.003975 0.03518 False High Sequest HT (A2) Unambiguous DAQGQPGLERAQQELVPPQQQASPPQLPK [K].dAQGQPGLERAQQELVPPQQQAsPPQLPk.[A] N-Term(TMT6plex); S23(Phospho); K29(TMT6plex) 5 O15027 O15027-5; O15027-4; O15027; O15027-2; O15027-3 1 4 0.3523 0 1 1 919.23301 3673.91023 3673.91034 -0.03 -3e-05 1997735.5 CID (Collision Induced Dissociation) MS2 6.981718 30 79.0 34.944 97.3722 31692 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 79.7 84.8 63.9 81.8 71.8 86.1 85.0 87.1 74.1 75.8 Excluded by Method 2.98 1 0.003975 0.03498 False High Sequest HT (A2) Unambiguous ELSPAGSISK [K].eLsPAGsISk.[N] N-Term(TMT6plex); S3(Phospho); S7(Phospho); K10(TMT6plex) 4 O95453 O95453-4; O95453-2; O95453-3; O95453 0 3 0.4249 0 1 1 536.26671 1606.78557 1606.78948 -2.43 -0.0013 582328.5625 CID (Collision Induced Dissociation) MS2 32.41803 30 73.4 60.0 78.0719 24525 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 77.7 72.5 61.0 73.6 67.3 76.3 91.0 72.5 72.0 69.7 Excluded by Method 3.53 1 0.003975 0.03484 False High Sequest HT (A2) Unambiguous STSAPQMSPGSSDNQSSSPQPAQQK [K].sTSAPQMsPGSSDNQSSSPQPAQQk.[L] N-Term(TMT6plex); S8(Phospho); K25(TMT6plex) 5 Q14157-1 Q14157-1; Q14157-5; Q14157-4; Q14157; Q14157-3 0 4 0.1167 0 1 1 768.36101 3070.4222 3070.41889 1.08 0.00083 862571.5 CID (Collision Induced Dissociation) MS2 40.18006 30 44.4 60.0 56.5028 16425 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 47.9 48.6 37.5 40.4 42.5 43.0 44.7 49.6 47.5 41.8 Excluded by Method 3.77 1 0.003975 0.03468 False High Sequest HT (A2) Unambiguous SPSLSPSPPSPLEK [K].sPsLsPSPPSPLEk.[T] N-Term(TMT6plex); S3(Phospho); S5(Phospho); K14(TMT6plex) 1 P46821 P46821 0 3 0.0256 0 1 1 681.00592 2041.00322 2041.00601 -1.37 -0.00093 1015772.9375 CID (Collision Induced Dissociation) MS2 0.0 40 127.9 60.0 96.1492 31263 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 136.4 127.9 101.0 119.0 117.1 130.3 144.1 152.7 126.9 123.3 Excluded by Method 3.52 1 0.004108 0.03542 False High Sequest HT (A2) Unambiguous EFSIPRAHAWPSPYK [K].eFsIPRAHAWPSPYk.[D] N-Term(TMT6plex); S3(Phospho); K15(TMT6plex) 1 Q8NFJ5 Q8NFJ5 1 3 0.3214 0 1 1 775.40452 2324.199 2324.19931 -0.13 -0.0001 780863.0625 CID (Collision Induced Dissociation) MS2 44.89351 50 20.5 60.0 100.5805 32982 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 22.0 20.1 17.0 22.1 19.5 20.7 16.0 22.7 23.7 20.8 Excluded by Method 2.52 1 0.004108 0.03557 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVsSDEEF.[-] N-Term(TMT6plex); S8(Phospho) 1 P16521 P16521 0 2 0.0395 0 1 1 885.37175 1769.73622 1769.73562 0.34 0.0003 223320.5 CID (Collision Induced Dissociation) MS2 0.0 50 29.3 60.0 140.0566 47809 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 10.2 24.8 48.0 9.8 26.0 54.2 10.9 24.6 53.2 31.1 Excluded by Method 2.53 1 0.004108 0.03577 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVssDEEF.[-] N-Term(TMT6plex); S8(Phospho); S9(Phospho) 1 P16521 P16521 0 2 0.2297 0 1 1 925.35516 1849.70305 1849.70195 0.59 0.00055 559061.75 CID (Collision Induced Dissociation) MS2 16.10803 40 49.3 60.0 143.837 49083 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 16.3 39.2 69.6 15.2 32.8 97.4 23.6 45.7 102.1 51.5 Excluded by Method 2.09 1 0.004108 0.03577 False High Sequest HT (A2) Unambiguous VSRVASPK [K].vSRVAsPk.[K] N-Term(TMT6plex); S6(Phospho); K8(TMT6plex) 1 P46821 P46821 1 3 0.4021 0 1 1 461.27065 1381.79739 1381.79687 0.38 0.00017 9303264.0 CID (Collision Induced Dissociation) MS2 3.699326 40 251.2 26.202 45.1654 12187 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 247.2 245.6 186.5 267.7 255.0 244.5 281.4 263.6 256.4 263.9 Excluded by Method 2.81 1 0.004224 0.03624 False High Sequest HT (A2) Unambiguous DTSFEHEK [K].dTsFEHEk.[E] N-Term(TMT6plex); S3(Phospho); K8(TMT6plex) 1 P40157 P40157 0 3 0.0091 0 1 1 510.91317 1530.72495 1530.72416 0.51 0.00026 2729258.0 CID (Collision Induced Dissociation) MS2 6.024961 20 55.7 60.0 52.2049 14774 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 15.6 47.5 83.1 23.2 45.3 97.0 21.6 54.3 110.5 59.1 Excluded by Method 2.19 1 0.004224 0.03634 False High Sequest HT (A2) Unambiguous KQSFDDNDSEELEDK [K].kQSFDDNDsEELEDk.[D] N-Term(TMT6plex); K1(TMT6plex); S9(Phospho); K15(TMT6plex) 1 O60841 O60841 1 3 0.1784 0 1 1 856.07643 2566.21473 2566.21648 -0.68 -0.00058 3062943.0 CID (Collision Induced Dissociation) MS2 0.0 50 37.5 60.0 74.0545 23014 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 39.2 42.4 27.5 48.1 35.5 32.8 46.4 38.9 33.3 30.8 Excluded by Method 3.7 1 0.004224 0.03718 False High Sequest HT (A2) Unambiguous NTANEDVTESTEDLK [K].nTANEDVTEStEDLk.[N] N-Term(TMT6plex); T11(Phospho); K15(TMT6plex) 1 Q12500 Q12500 0 3 0.0068 0 1 1 735.35047 2204.03687 2204.03717 -0.14 -0.0001 791668.6875 CID (Collision Induced Dissociation) MS2 26.91825 40 9.2 60.0 67.159 20345 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 3.2 8.3 8.3 3.5 9.4 12.5 9.0 10.9 15.1 11.8 Excluded by Method 1.47 1 0.004224 0.03676 False High Sequest HT (A2) Unambiguous IFGSRVRVDSTAK [K].iFGSRVRVDsTAk.[V] N-Term(TMT6plex); S10(Phospho); K13(TMT6plex) 2 P78371 P78371-2; P78371 2 4 0.172 0 1 1 494.279 1974.09417 1974.09378 0.2 0.0001 901962.25 CID (Collision Induced Dissociation) MS2 16.87585 10 38.3 60.0 72.0383 22195 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 30.1 28.6 37.2 37.0 43.8 37.6 42.2 43.1 39.0 44.2 Excluded by Method 1.57 1 0.004224 0.03718 False High Sequest HT (A2) Unambiguous APKSPEVIEID [K].aPksPEVIEID.[-] N-Term(TMT6plex); K3(TMT6plex); S4(Phospho) 1 P53044 P53044 1 3 0.4575 0 1 1 579.31404 1735.92758 1735.92834 -0.44 -0.00025 748302.25 CID (Collision Induced Dissociation) MS2 5.717676 40 332.9 60.0 91.611 29558 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 77.0 255.0 520.6 110.7 271.1 643.3 124.5 291.2 674.7 360.5 Excluded by Method 2.12 1 0.004224 0.03705 False High Sequest HT (A2) Unambiguous EEPPPARSGSGGGSAK [K].eEPPPARSGsGGGSAk.[E] N-Term(TMT6plex); S10(Phospho); K16(TMT6plex) 5 Q9H4A3 Q9H4A3-5; Q9H4A3-2; Q9H4A3-7; Q9H4A3; Q9H4A3-6 1 3 0.0679 0 1 1 674.67414 2022.00788 2022.00575 1.05 0.00071 173132.484375 CID (Collision Induced Dissociation) MS2 30.38057 20 84.5 60.0 36.4924 9109 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 90.4 86.4 70.2 90.0 89.1 92.0 86.2 88.5 75.3 77.1 Excluded by Method 2.65 1 0.004224 0.03613 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVssDEEF.[-] N-Term(TMT6plex); S8(Phospho); S9(Phospho) 1 P16521 P16521 0 2 0.1612 0 1 1 925.35635 1849.70543 1849.70195 1.88 0.00174 429985.96875 CID (Collision Induced Dissociation) MS2 31.14233 30 76.2 60.0 143.6074 49047 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 17.8 60.7 122.0 23.0 65.4 144.6 21.8 67.0 156.5 83.1 Excluded by Method 2.42 1 0.004224 0.03766 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVssDEEF.[-] N-Term(TMT6plex); S8(Phospho); S9(Phospho) 1 P16521 P16521 0 2 0.1706 0 1 1 925.35236 1849.69744 1849.70195 -2.44 -0.00226 652018.875 CID (Collision Induced Dissociation) MS2 13.31295 40 57.0 60.0 134.8224 45983 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 23.9 54.2 83.1 18.5 45.2 101.9 24.3 56.5 108.8 53.3 Excluded by Method 2.52 1 0.004224 0.03633 False High Sequest HT (A2) Unambiguous WFFISGSV [K].wFFISGsV.[-] N-Term(TMT6plex); S7(Phospho) 1 P00817 P00817 0 2 0.1389 0 1 1 626.30465 1251.60203 1251.60124 0.62 0.00039 180349.796875 CID (Collision Induced Dissociation) MS2 0.0 50 28.6 60.0 144.0579 49204 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 9.5 23.9 53.9 9.7 21.1 53.5 9.3 23.1 50.8 31.6 Excluded by Method 1.8 1 0.004336 0.03795 False High Sequest HT (A2) Unambiguous AYSPTCSPTLGFK [K].aYsPTcsPTLGFk.[E] N-Term(TMT6plex); S3(Phospho); C6(Carbamidomethyl); S7(Phospho); K13(TMT6plex) 1 Q5TGY3 Q5TGY3 0 3 0.0689 0 1 1 682.98608 2046.9437 2046.9413 1.17 0.0008 654288.3125 CID (Collision Induced Dissociation) MS2 10.48442 30 158.6 60.0 107.4676 35505 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 155.0 152.8 154.2 155.6 146.7 153.4 178.1 171.7 157.5 160.5 Excluded by Method 3.05 1 0.004336 0.03916 False High Sequest HT (A2) Unambiguous IELPPLSPK [K].iELPPLsPk.[S] N-Term(TMT6plex); S7(Phospho); K9(TMT6plex) 1 P40036 P40036 0 3 0.1737 0 1 1 511.30165 1531.89041 1531.8901 0.2 0.0001 277283.40625 CID (Collision Induced Dissociation) MS2 30.59197 40 12.4 60.0 115.6089 38530 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 3.8 10.3 21.0 8.3 9.6 19.2 7.4 12.5 18.9 12.9 Excluded by Method 1.67 1 0.004336 0.0392 False High Sequest HT (A2) Unambiguous EEEAKEESDDDMGFGLFD [K].eEEAkEEsDDDmGFGLFD.[-] N-Term(TMT6plex); K5(TMT6plex); S8(Phospho); M12(Oxidation) 1 P02400 P02400 1 3 0.5153 0 1 1 873.0368 2617.09584 2617.0941 0.67 0.00058 2775403.0 CID (Collision Induced Dissociation) MS2 5.328568 40 171.8 37.674 120.6483 40440 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 45.1 150.7 236.3 49.1 153.0 316.6 61.1 162.8 367.3 175.6 Excluded by Method 2.62 1 0.004336 0.03909 False High Sequest HT (A2) Unambiguous EEEAAEESDDDMGFGLFD [K].eEEAAEEsDDDmGFGLFD.[-] N-Term(TMT6plex); S8(Phospho); M12(Oxidation) 2 P05319 P10622; P05319 0 3 0.6082 0 1 1 777.63199 2330.88142 2330.87332 3.48 0.0027 5317502.5 CID (Collision Induced Dissociation) MS2 29.44319 30 72.1 12.002 130.9188 44512 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 29.4 60.5 105.2 29.0 54.1 129.2 31.0 72.8 135.5 74.8 Excluded by Method 2.68 1 0.004336 0.03857 False High Sequest HT (A2) Unambiguous DDDEEPSDSEDK [K].dDDEEPsDSEDk.[E] N-Term(TMT6plex); S7(Phospho); K12(TMT6plex) 1 P09232 P09232 0 2 0.0977 0 1 1 959.89907 1918.79087 1918.78431 3.42 0.00328 143077.453125 CID (Collision Induced Dissociation) MS2 0.0 50 12.7 60.0 43.5972 11663 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 2.1 9.6 17.0 1.9 13.2 22.8 6.1 14.9 24.3 15.5 Excluded by Method 2.66 1 0.004336 0.03876 False High Sequest HT (A2) Unambiguous LSGFSFK [K].lSGFsFk.[K] N-Term(TMT6plex); S5(Phospho); K7(TMT6plex) 1 P29966 P29966 0 2 0.407 0 1 1 662.35934 1323.71141 1323.71141 0.0 0.0 2696076.5 CID (Collision Induced Dissociation) MS2 10.85711 10 61.6 47.006 114.9519 38291 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 35.9 194.6 26.7 94.6 35.0 36.3 44.6 42.4 41.7 64.2 Excluded by Method 2.58 1 0.004336 0.03835 False High Sequest HT (A2) Unambiguous VVHRTATADDK [K].vVHRTAtADDk.[K] N-Term(TMT6plex); T7(Phospho); K11(TMT6plex) 4 P20290 P20290-2; Q96K17-3; Q96K17; P20290 1 3 0.0798 0 1 1 584.31291 1750.92416 1750.92532 -0.66 -0.00039 67225.7734375 CID (Collision Induced Dissociation) MS2 10.47581 20 11.9 60.0 33.3115 7914 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 12.0 13.0 5.7 12.1 11.4 14.1 13.0 12.1 11.0 14.9 Excluded by Method 1.88 1 0.004336 0.03884 False High Sequest HT (A2) Unambiguous EEDEEPESPPEK [K].eEDEEPEsPPEk.[K] N-Term(TMT6plex); S8(Phospho); K12(TMT6plex) 2 Q9H1E3 Q9H1E3; Q9H1E3-2 0 4 0.1311 0 1 1 488.97513 1952.87868 1952.87782 0.44 0.00022 3315669.0 CID (Collision Induced Dissociation) MS2 0.0 50 260.5 38.776 55.581 16029 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 251.3 240.5 199.1 299.6 257.9 241.9 294.6 270.2 273.8 276.0 Excluded by Method 3.51 1 0.004336 0.03836 False High Sequest HT (A2) Unambiguous SFEVTDPVNSSLK [K].sFEVtDPVNSSLk.[G] N-Term(TMT6plex); T5(Phospho); K13(TMT6plex) 1 P54838 P54838 0 3 0.1314 0 1 1 654.33903 1961.00254 1961.0033 -0.38 -0.00025 622087.5625 CID (Collision Induced Dissociation) MS2 59.19168 50 46.3 60.0 104.6317 34498 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 24.1 42.0 59.3 23.1 38.4 78.5 37.6 38.0 75.1 47.1 Excluded by Method 2.36 1 0.004336 0.03841 False High Sequest HT (A2) Unambiguous EEEAKEESDDDMGFGLFD [K].eEEAkEEsDDDmGFGLFD.[-] N-Term(TMT6plex); K5(TMT6plex); S8(Phospho); M12(Oxidation) 1 P02400 P02400 1 2 0.4615 0 1 1 1309.05219 2617.09711 2617.0941 1.15 0.0015 1714877.375 CID (Collision Induced Dissociation) MS2 0.0 30 116.4 60.0 114.4587 38075 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 29.7 97.4 171.5 38.5 97.2 220.2 38.4 110.6 243.9 116.6 Excluded by Method 2.73 1 0.004336 0.03959 False High Sequest HT (A2) Unambiguous EPSRERDIAK [K].ePsRERDIAk.[E] N-Term(TMT6plex); S3(Phospho); K10(TMT6plex) 2 Q8NI27 Q8NI27; Q8NI27-2 2 3 0.0796 0 1 1 580.31337 1738.92556 1738.92532 0.14 8e-05 4756693.5 CID (Collision Induced Dissociation) MS2 4.130244 30 100.3 28.275 41.2009 10807 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 98.2 95.5 76.2 101.5 100.4 103.5 109.9 114.7 96.7 106.4 Excluded by Method 2.26 1 0.004336 0.03841 False High Sequest HT (A2) Unambiguous NAEEESESEAEEGD [K].nAEEEsEsEAEEGD.[-] N-Term(TMT6plex); S6(Phospho); S8(Phospho) 4 Q7L1Q6 Q7L1Q6-2; Q7L1Q6-4; Q7L1Q6-3; Q7L1Q6 0 2 0.8125 0 1 1 957.32391 1913.64055 1913.6412 -0.34 -0.00033 143013.875 CID (Collision Induced Dissociation) MS2 25.48159 30 9.4 60.0 40.1502 10426 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 8.6 6.7 8.0 10.7 11.5 11.4 10.5 8.1 12.8 6.0 Excluded by Method 1.28 1 0.004336 0.03846 False High Sequest HT (A2) Unambiguous EALAFDNDESDAQDDANNEK [K].eALAFDNDEsDAQDDANNEk.[E] N-Term(TMT6plex); S10(Phospho); K20(TMT6plex) 1 P38968 P38968 0 4 0.4553 0 1 1 688.05191 2749.18583 2749.18781 -0.72 -0.0005 1005557.875 CID (Collision Induced Dissociation) MS2 16.95254 10 41.4 33.463 84.9714 27037 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 26.8 38.6 40.9 32.9 42.1 55.3 35.8 41.9 60.6 38.7 Excluded by Method 3.69 1 0.004336 0.03802 False High Sequest HT (A2) Unambiguous GSILLTSDEEEEEK [K].gSILLTsDEEEEEk.[G] N-Term(TMT6plex); S7(Phospho); K14(TMT6plex) 1 P46949 P46949 0 3 0.0325 0 1 1 706.34778 2117.02879 2117.0303 -0.71 -0.0005 1561800.75 CID (Collision Induced Dissociation) MS2 0.0 60 50.3 60.0 92.5239 29874 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 33.1 54.5 51.6 38.0 36.7 71.0 36.5 44.4 77.4 60.1 Excluded by Method 2.46 1 0.004336 0.0379 False High Sequest HT (A2) Unambiguous GVIPERAPSPTAK [K].gVIPERAPsPTAk.[L] N-Term(TMT6plex); S9(Phospho); K13(TMT6plex) 1 Q12017 Q12017 1 4 0.0601 0 1 1 466.01407 1861.03443 1861.03487 -0.23 -0.00011 785481.1875 CID (Collision Induced Dissociation) MS2 0.0 40 80.2 60.0 67.956 20709 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 23.1 64.0 123.4 26.5 76.9 157.5 32.5 75.5 147.7 75.1 Excluded by Method 2.33 1 0.004336 0.03931 False High Sequest HT (A2) Unambiguous IRNQQQRQSHENIEK [K].iRNQQQRQsHENIEk.[L] N-Term(TMT6plex); S9(Phospho); K15(TMT6plex) 1 P13185 P13185 2 4 0.293 0 1 1 612.32328 2446.27128 2446.27164 -0.15 -9e-05 147805.96875 CID (Collision Induced Dissociation) MS2 0.0 10 5.1 60.0 35.6667 8814 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 1.5 3.1 7.7 4.1 10.9 1.5 6.9 11.8 3.8 Excluded by Method 2.15 1 0.004336 0.03805 False High Sequest HT (A2) Unambiguous NDDSHDDLILLSAK [K].nDDsHDDLILLSAk.[K] N-Term(TMT6plex); S4(Phospho); K14(TMT6plex) 1 Q06673 Q06673 0 3 0.092 0 1 1 698.68925 2094.05319 2094.05204 0.55 0.00039 961214.5625 CID (Collision Induced Dissociation) MS2 14.66793 60 31.4 60.0 102.01 33459 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 17.7 27.0 43.4 26.9 20.6 46.7 22.1 35.1 48.8 26.0 Excluded by Method 1.74 1 0.004454 0.04099 False High Sequest HT (A2) Unambiguous SLKESEQESEEEILAQK [K].sLkEsEQEsEEEILAQk.[K] N-Term(TMT6plex); K3(TMT6plex); S5(Phospho); S9(Phospho); K17(TMT6plex) 1 Q9BW71 Q9BW71 1 3 0.1637 0 1 1 942.13314 2824.38486 2824.38733 -0.88 -0.00082 522193.34375 CID (Collision Induced Dissociation) MS2 10.78154 60 39.5 60.0 94.7775 30746 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 40.1 33.5 28.2 42.8 36.8 44.2 42.7 45.6 40.2 41.0 Excluded by Method 3.36 1 0.004454 0.04051 False High Sequest HT (A2) Unambiguous SLQNFQFPK [K].sLQNFQFPk.[N] N-Term(TMT6plex); S1(Phospho); K9(TMT6plex) 1 P40048 P40048 0 3 0.2896 0 1 1 549.62876 1646.87174 1646.87077 0.59 0.00033 207533.234375 CID (Collision Induced Dissociation) MS2 64.66348 20 48.0 60.0 109.8828 36377 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 16.1 39.7 76.0 21.7 41.8 84.4 20.2 48.5 78.1 53.5 Excluded by Method 2.97 1 0.004454 0.04154 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVSsDEEF.[-] N-Term(TMT6plex); S9(Phospho) 1 P16521 P16521 0 2 0.0568 0 1 1 885.37169 1769.73609 1769.73562 0.27 0.00024 14095670.0 CID (Collision Induced Dissociation) MS2 70.06023 10 82.6 60.0 112.6449 37405 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 28.8 61.9 113.4 38.2 80.5 143.8 39.1 88.4 144.2 87.8 Excluded by Method 2.29 1 0.004454 0.04042 False High Sequest HT (A2) Unambiguous KQSFDDNDSEELEDK [K].kQSFDDNDsEELEDk.[D] N-Term(TMT6plex); K1(TMT6plex); S9(Phospho); K15(TMT6plex) 1 O60841 O60841 1 3 0.1095 0 1 1 856.07572 2566.2126 2566.21648 -1.51 -0.00129 1316638.5 CID (Collision Induced Dissociation) MS2 27.00192 40 17.2 60.0 74.6626 23155 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 15.0 17.9 18.4 18.6 19.0 16.7 11.0 17.7 20.5 16.8 Excluded by Method 2.1 1 0.004454 0.04106 False High Sequest HT (A2) Unambiguous EEEAKEESDDDMGFGLFD [K].eEEAkEEsDDDmGFGLFD.[-] N-Term(TMT6plex); K5(TMT6plex); S8(Phospho); M12(Oxidation) 1 P02400 P02400 1 2 0.4682 0 1 1 1309.05294 2617.0986 2617.0941 1.72 0.00225 414698.6875 CID (Collision Induced Dissociation) MS2 0.0 30 29.3 60.0 115.7763 38625 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 9.5 21.7 37.3 12.0 26.1 56.0 8.8 28.1 64.1 29.1 Excluded by Method 2.2 1 0.004454 0.04134 False High Sequest HT (A2) Unambiguous ASDSEEPEEK [K].asDSEEPEEk.[Q] N-Term(TMT6plex); S2(Phospho); K10(TMT6plex) 4 Q9C0E8 Q9C0E8-2; Q9C0E8; Q9C0E8-4; Q9C0E8-3 0 2 0.283 0 1 1 829.88188 1658.75649 1658.75625 0.15 0.00012 803662.5625 CID (Collision Induced Dissociation) MS2 0.0 40 56.2 60.0 44.9803 12166 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 59.3 54.6 52.3 44.0 50.9 59.6 54.5 61.6 53.8 71.5 Excluded by Method 2.12 1 0.004454 0.04012 False High Sequest HT (A2) Unambiguous GLSPLEEEK [K].gLsPLEEEk.[L] N-Term(TMT6plex); S3(Phospho); K9(TMT6plex) 1 P31374 P31374 0 3 0.3846 0 1 1 513.94017 1539.80596 1539.80716 -0.78 -0.0004 674049.875 CID (Collision Induced Dissociation) MS2 0.0 40 71.2 60.0 87.3902 27944 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 24.9 64.0 90.4 31.9 65.1 122.3 33.2 64.5 127.6 88.2 Excluded by Method 3.51 1 0.004454 0.04026 False High Sequest HT (A2) Unambiguous NAEEESESEAEEGD [K].nAEEEsEsEAEEGD.[-] N-Term(TMT6plex); S6(Phospho); S8(Phospho) 4 Q7L1Q6 Q7L1Q6-2; Q7L1Q6-4; Q7L1Q6-3; Q7L1Q6 0 2 0.8841 0 1 1 957.32492 1913.64256 1913.6412 0.71 0.00068 77307.9921875 CID (Collision Induced Dissociation) MS2 0.0 50 2.9 60.0 37.0877 9317 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 1.9 2.6 2.7 2.7 3.0 3.4 2.6 1.8 7.8 Excluded by Method 1.64 1 0.004454 0.04172 False High Sequest HT (A2) Unambiguous VMIGSPK [K].vMIGsPk.[K] N-Term(TMT6plex); S5(Phospho); K7(TMT6plex) 2 Q68CZ2 Q68CZ2; Q68CZ2-2 0 2 0.1609 0 1 1 635.35608 1269.70489 1269.70422 0.53 0.00034 2174974.5 CID (Collision Induced Dissociation) MS2 3.448554 30 157.8 60.0 77.1936 24142 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 142.2 160.0 121.6 167.5 153.9 158.8 183.9 171.0 156.8 162.0 Excluded by Method 2.61 1 0.004454 0.04167 False High Sequest HT (A2) Unambiguous DMSPLSETEMALGK [K].dMSPLSEtEMALGk.[D] N-Term(TMT6plex); T8(Phospho); K14(TMT6plex) 3 P27816 P27816; P27816-6; P27816-2 0 3 0.0503 0 1 1 683.00104 2046.98856 2046.9893 -0.36 -0.00025 373972.65625 CID (Collision Induced Dissociation) MS2 12.77873 30 76.1 60.0 126.0597 42376 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 90.5 75.6 57.4 68.8 70.4 80.5 86.0 78.5 79.2 74.4 Excluded by Method 1.79 1 0.004529 0.04361 False High Sequest HT (A2) Unambiguous AEFTSPPSLFK [K].aEFTsPPSLFk.[T] N-Term(TMT6plex); S5(Phospho); K11(TMT6plex) 4 Q6UN15 Q6UN15-4; Q6UN15-5; Q6UN15-3; Q6UN15 0 3 0.0729 0 1 1 587.9791 1761.92274 1761.92286 -0.07 -4e-05 1165654.625 CID (Collision Induced Dissociation) MS2 1.763993 60 139.8 60.0 121.0087 40490 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 139.2 150.2 126.0 133.9 128.5 140.1 158.1 144.0 142.1 136.2 Excluded by Method 2.47 1 0.004529 0.04616 False High Sequest HT (A2) Unambiguous LGFSSSPTK [K].lGFSSsPTk.[K] N-Term(TMT6plex); S6(Phospho); K9(TMT6plex) 3 Q8IVF2 Q8IVF2-3; Q8IVF2-2; Q8IVF2 0 2 0.0325 0 1 1 731.3912 1461.77512 1461.77547 -0.24 -0.00018 6379736.0 CID (Collision Induced Dissociation) MS2 40.44028 30 155.3 30.099 82.9553 26364 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 159.5 147.4 115.2 168.2 146.8 156.2 186.6 155.6 157.6 159.4 Excluded by Method 2.46 1 0.004529 0.0431 False High Sequest HT (A2) Unambiguous IGEGTYGVVYK [K].iGEGtyGVVYk.[AG] N-Term(TMT6plex); T5(Phospho); Y6(Phospho); K11(TMT6plex) 5 P24941 P24941; P06493; Q00526; P24941-2; P06493-2 0 3 0.3378 0 1 1 601.96245 1803.8728 1803.87354 -0.41 -0.00025 124866.9921875 CID (Collision Induced Dissociation) MS2 71.73402 40 51.6 60.0 98.5091 32150 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 37.6 86.3 38.2 56.0 38.8 55.7 44.7 50.9 59.8 48.3 Excluded by Method 2.96 1 0.004529 0.04313 False High Sequest HT (A2) Unambiguous DLFDYSPPLHK [K].dLFDYsPPLHk.[N] N-Term(TMT6plex); S6(Phospho); K11(TMT6plex) 3 Q9NYF8 Q9NYF8-3; Q9NYF8; Q9NYF8-2 0 3 0.043 0 1 1 623.98946 1869.95382 1869.95522 -0.75 -0.00047 421072.71875 CID (Collision Induced Dissociation) MS2 47.9584 30 99.1 60.0 108.9732 36066 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 88.3 83.0 92.7 86.4 95.0 123.1 97.3 107.7 125.6 91.8 Excluded by Method 2.79 1 0.004529 0.04396 False High Sequest HT (A2) Unambiguous WFFISGSV [K].wFFISGsV.[-] N-Term(TMT6plex); S7(Phospho) 1 P00817 P00817 0 2 0.1091 0 1 1 626.30465 1251.60203 1251.60124 0.62 0.00039 152756.421875 CID (Collision Induced Dissociation) MS2 20.09277 50 21.6 60.0 146.6254 50098 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 3.6 22.5 32.4 5.9 14.1 37.9 10.3 21.2 48.8 18.9 Excluded by Method 1.65 1 0.004529 0.04587 False High Sequest HT (A2) Unambiguous MASRRSEEK [K].mASRRsEEk.[E] N-Term(TMT6plex); S6(Phospho); K9(TMT6plex) 2 Q9NQ29 Q9NQ29-3; Q9NQ29 2 3 0.1885 0 1 1 544.61652 1631.835 1631.83406 0.58 0.00031 129544.921875 CID (Collision Induced Dissociation) MS2 0.0 10 19.9 60.0 27.4033 5920 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 22.4 14.9 16.5 22.0 20.0 19.5 23.6 24.3 16.7 19.0 Excluded by Method 1.22 1 0.004529 0.04205 False High Sequest HT (A2) Unambiguous EDQEEALSDNEPESSEK [K].eDQEEALsDNEPESSEk.[F] N-Term(TMT6plex); S8(Phospho); K17(TMT6plex) 1 P34250 P34250 0 3 0.3068 0 1 1 825.3651 2474.08074 2474.08597 -2.12 -0.00175 583128.625 CID (Collision Induced Dissociation) MS2 0.0 30 14.5 60.0 61.446 18272 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 8.7 13.7 18.8 7.5 11.9 20.3 9.4 13.6 26.2 14.5 Excluded by Method 1.76 1 0.004529 0.04255 False High Sequest HT (A2) Unambiguous WLISPVK [K].wLIsPVk.[I] N-Term(TMT6plex); S4(Phospho); K7(TMT6plex) 3 Q8NCD3 Q8NCD3-2; Q8NCD3-3; Q8NCD3 0 2 0.4553 0 1 1 690.90648 1380.80569 1380.80565 0.03 2e-05 4763082.0 CID (Collision Induced Dissociation) MS2 34.5074 30 177.3 55.121 118.7618 39694 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 186.2 173.1 145.4 187.2 176.0 160.0 202.1 189.9 174.3 178.6 Excluded by Method 2.46 1 0.004529 0.04537 False High Sequest HT (A2) Unambiguous RPASPSSPEHLPATPAESPAQRFEARIEDGK [K].rPAsPsSPEHLPATPAESPAQRFEARIEDGk.[L] N-Term(TMT6plex); S4(Phospho); S6(Phospho); K31(TMT6plex) 1 Q9H7L9 Q9H7L9 2 5 0.0765 0 1 1 790.19131 3946.92742 3946.92542 0.51 0.0004 725095.9375 CID (Collision Induced Dissociation) MS2 0.0 30 33.1 60.0 88.6674 28503 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 31.4 38.1 23.3 41.0 37.2 29.8 30.9 35.4 33.8 29.7 Excluded by Method 3.92 1 0.004529 0.04725 False High Sequest HT (A2) Unambiguous NTLETSSLNFK [K].nTLETSsLNFk.[V] N-Term(TMT6plex); S7(Phospho); K11(TMT6plex) 1 Q14151 Q14151 0 3 0.0541 0 1 1 597.98143 1791.92972 1791.9294 0.18 0.00011 1328777.375 CID (Collision Induced Dissociation) MS2 14.6124 40 55.5 60.0 94.7598 30789 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 50.3 54.2 42.4 61.5 51.8 58.6 62.6 60.2 59.1 53.9 Excluded by Method 2.22 1 0.004529 0.04379 False High Sequest HT (A2) Unambiguous KPTPLPSLK [K].kPtPLPSLk.[D] N-Term(TMT6plex); K1(TMT6plex); T3(Phospho); K9(TMT6plex) 1 P06105 P06105 0 3 0.37 0 1 1 583.3609 1748.06814 1748.06902 -0.51 -0.00029 1670008.625 CID (Collision Induced Dissociation) MS2 5.488984 50 99.0 60.0 81.7642 25914 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 30.3 80.6 154.7 24.8 89.2 195.7 39.3 90.6 177.6 106.9 Excluded by Method 3.0 1 0.004529 0.04625 False High Sequest HT (A2) Unambiguous SLKESEQESEEEILAQK [K].sLkEsEQEsEEEILAQk.[K] N-Term(TMT6plex); K3(TMT6plex); S5(Phospho); S9(Phospho); K17(TMT6plex) 1 Q9BW71 Q9BW71 1 3 0.105 0 1 1 942.13269 2824.38352 2824.38733 -1.35 -0.00127 344108.0625 CID (Collision Induced Dissociation) MS2 31.10512 40 22.7 60.0 95.8677 31123 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 21.3 19.8 18.2 24.9 23.2 25.7 23.5 25.3 22.7 22.1 Excluded by Method 2.38 1 0.004529 0.04268 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVSsDEEF.[-] N-Term(TMT6plex); S9(Phospho) 1 P16521 P16521 0 2 0.0819 0 1 1 885.37108 1769.73487 1769.73562 -0.42 -0.00037 181625.171875 CID (Collision Induced Dissociation) MS2 28.62341 50 31.9 60.0 142.7097 48734 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 10.6 21.4 51.3 8.3 25.6 60.4 11.0 26.0 69.2 35.2 Excluded by Method 1.71 1 0.004529 0.04564 False High Sequest HT (A2) Unambiguous QGGSQPSSFSPGQSQVTPQDQEK [K].qGGSQPSSFsPGQSQVTPQDQEk.[A] N-Term(TMT6plex); S10(Phospho); K23(TMT6plex) 1 Q9H0L4 Q9H0L4 0 3 0.0462 0 1 1 981.4696 2942.39423 2942.39333 0.31 0.0003 581022.625 CID (Collision Induced Dissociation) MS2 48.505 60 7.5 60.0 67.9818 20654 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 4.0 7.5 3.5 6.7 8.6 6.7 9.4 7.3 11.4 9.6 Excluded by Method 1.95 1 0.004529 0.04752 False High Sequest HT (A2) Unambiguous VSGTLDTPEK [K].vSGTLDtPEk.[T] N-Term(TMT6plex); T7(Phospho); K10(TMT6plex) 1 Q9BYG3 Q9BYG3 0 3 0.2482 0 1 1 528.94789 1584.82913 1584.82863 0.32 0.00017 999024.3125 CID (Collision Induced Dissociation) MS2 0.0 40 186.0 60.0 67.9543 20645 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 182.2 181.4 145.7 210.7 175.3 171.5 218.1 210.5 176.6 188.0 Excluded by Method 2.74 1 0.004529 0.04476 False High Sequest HT (A2) Unambiguous TTRTPEEGGYSYDISEK [K].tTRtPEEGGYSYDISEk.[T] N-Term(TMT6plex); T4(Phospho); K17(TMT6plex) 1 P46821 P46821 1 3 0.0317 0 1 1 824.3988 2471.18184 2471.17434 3.04 0.0025 915161.375 CID (Collision Induced Dissociation) MS2 14.72324 30 42.7 60.0 70.9218 21775 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 45.4 37.1 33.1 41.7 40.9 50.4 44.4 44.0 46.5 43.7 Excluded by Method 3.78 1 0.004529 0.04321 False High Sequest HT (A2) Unambiguous IGVADAINELLEPIRQEFANNK [K].iGVADAINELLEPIRQEFANNk.[E] N-Term(TMT6plex); K22(TMT6plex) 1 P36421 P36421 1 3 0.4662 0 1 1 971.54646 2912.62482 2912.62458 0.08 8e-05 7397779.5 CID (Collision Induced Dissociation) MS2 39.9035 40 5.7 11.714 131.1028 44532 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 8.5 9.7 2.5 10.7 3.8 6.0 12.8 3.0 Excluded by Method 2.81 1 0.004529 0.04489 False High Sequest HT (A2) Unambiguous AIGTAYAVLSNPEKR [K].aIGTAyAVLSNPEkR.[K] N-Term(TMT6plex); Y6(Phospho); K14(TMT6plex) 2 Q9NXW2 Q9NXW2; Q9NXW2-2 1 3 0.0236 0 1 1 710.05758 2128.15819 2128.15678 0.66 0.00047 3297451.25 CID (Collision Induced Dissociation) MS2 6.44459 30 104.1 60.0 74.4679 23104 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 104.0 107.5 77.2 134.0 85.6 83.2 144.1 109.1 83.7 112.3 Excluded by Method 2.96 1 0.004529 0.04615 False High Sequest HT (A2) Unambiguous GTEENSEIESFK [K].gTEENSEIEsFk.[S] N-Term(TMT6plex); S10(Phospho); K12(TMT6plex) 1 P53890 P53890 0 2 0.363 0 1 1 954.457 1907.90673 1907.90398 1.44 0.00138 400906.46875 CID (Collision Induced Dissociation) MS2 51.09232 10 24.8 60.0 76.7466 24024 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 3.1 23.7 30.6 11.0 20.3 43.8 8.0 27.0 53.7 26.6 Excluded by Method 2.92 1 0.004529 0.04213 False High Sequest HT (A2) Unambiguous GSQESTNTLEEIVK [K].gSQEStNtLEEIVk.[F] N-Term(TMT6plex); T6(Phospho); T8(Phospho); K14(TMT6plex) 1 P35845 P35845 0 3 0.0366 0 1 1 718.34427 2153.01827 2153.01803 0.11 8e-05 573491.75 CID (Collision Induced Dissociation) MS2 17.74756 20 81.3 60.0 103.6391 34128 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 21.9 70.1 124.7 28.2 64.5 151.3 32.3 84.4 156.4 79.3 Excluded by Method 3.82 1 0.004529 0.04237 False High Sequest HT (A2) Unambiguous SVESTSPEPSK [K].sVESTsPEPSk.[I] N-Term(TMT6plex); S6(Phospho); K11(TMT6plex) 10 P18583 P18583; P18583-4; P18583-6; P18583-5; P18583-10; P18583-9; P18583-2; P18583-3; P18583-7; P18583-8 0 2 0.0897 0 1 1 843.42532 1685.84337 1685.83992 2.05 0.00172 374078.71875 CID (Collision Induced Dissociation) MS2 0.0 10 271.0 60.0 49.8849 13952 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 262.8 271.0 233.4 273.1 257.2 276.7 303.4 264.8 286.0 282.0 Excluded by Method 3.12 1 0.004529 0.04295 False High Sequest HT (A2) Unambiguous EESYSPSK [K].eESYsPSk.[D] N-Term(TMT6plex); S5(Phospho); K8(TMT6plex) 3 Q96T23 Q96T23; Q96T23-3; Q96T23-2 0 2 0.057 0 1 1 732.85518 1464.70309 1464.70236 0.5 0.00036 1293443.25 CID (Collision Induced Dissociation) MS2 37.06617 20 169.2 51.321 50.209 14081 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 158.6 162.6 150.9 166.0 158.9 171.5 177.8 175.8 209.4 160.4 Excluded by Method 2.28 1 0.004529 0.04595 False High Sequest HT (A2) Unambiguous AVEARSPGLQK [K].aVEARsPGLQk.[E] N-Term(TMT6plex); S6(Phospho); K11(TMT6plex) 4 Q3KQU3 Q3KQU3-3; Q3KQU3-2; Q3KQU3-4; Q3KQU3 1 3 0.427 0 1 1 565.3198 1693.94484 1693.94024 2.72 0.00153 736972.625 CID (Collision Induced Dissociation) MS2 3.854361 20 231.2 60.0 54.6668 15720 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 219.9 222.4 223.8 211.8 207.6 244.6 241.2 252.9 228.0 259.8 Excluded by Method 2.81 1 0.004529 0.0435 False High Sequest HT (A2) Unambiguous SGDEGSEDEAPSGED [K].sGDEGsEDEAPsGED.[-] N-Term(TMT6plex); S6(Phospho); S12(Phospho) 2 Q9H1E3 Q9H1E3; Q9H1E3-2 0 2 0.8281 0 1 1 935.31123 1869.61519 1869.61499 0.1 0.0001 112261.1171875 CID (Collision Induced Dissociation) MS2 0.0 50 9.4 60.0 37.981 9586 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 7.6 11.0 7.2 9.3 10.4 11.2 8.5 7.0 11.0 10.7 Excluded by Method 1.28 1 0.004529 0.04449 False High Sequest HT (A2) Unambiguous GSSERGSPIEK [K].gSSERGsPIEk.[Y] N-Term(TMT6plex); S7(Phospho); K11(TMT6plex) 3 Q9UHF7 Q9UHF7-3; Q9UHF7-2; Q9UHF7 1 3 0.204 0 1 1 562.29396 1684.86734 1684.86714 0.12 7e-05 932717.75 CID (Collision Induced Dissociation) MS2 10.0555 30 199.9 60.0 40.7024 10619 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 193.4 208.2 156.3 200.7 189.8 206.5 217.9 228.1 202.8 195.6 Excluded by Method 3.48 1 0.004654 0.0477 False High Sequest HT (A2) Unambiguous LLYPVKSESSTV [K].lLYPVksESSTV.[-] N-Term(TMT6plex); K6(TMT6plex); S7(Phospho) 1 P53860 P53860 1 2 0.0166 0 1 1 931.00956 1861.01183 1861.0124 -0.31 -0.00028 469528.21875 CID (Collision Induced Dissociation) MS2 44.9059 60 34.8 60.0 108.6423 35945 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 14.2 27.6 45.5 17.6 32.6 54.2 21.0 36.8 61.5 37.0 Excluded by Method 1.81 1 0.004654 0.04813 False High Sequest HT (A2) Unambiguous IALESEGRPEEQMESDNCSGGDDDWTHLSSK [K].iALESEGRPEEQmESDNcsGGDDDWTHLSSk.[E] N-Term(TMT6plex); M13(Oxidation); C18(Carbamidomethyl); S19(Phospho); K31(TMT6plex) 2 Q13501 Q13501-2; Q13501 0 4 0.1569 0 1 1 1009.19365 4033.75279 4033.74691 1.46 0.00147 236330.6875 CID (Collision Induced Dissociation) MS2 19.91821 60 3.3 60.0 93.2779 30184 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 2.9 6.8 3.4 2.3 7.1 2.8 3.0 2.8 2.0 Excluded by Method 3.57 1 0.004654 0.04815 False High Sequest HT (A2) Unambiguous WQPLPPEPLDTTPTK [K].wQPLPPEPLDTtPTk.[V] N-Term(TMT6plex); T12(Phospho); K15(TMT6plex) 1 Q08412 Q08412 0 3 0.0376 0 1 1 753.40024 2258.18615 2258.18741 -0.56 -0.00042 374774.28125 CID (Collision Induced Dissociation) MS2 22.68256 40 24.1 60.0 116.5586 38879 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 12.8 21.3 31.1 16.1 21.4 34.5 18.8 25.7 38.4 21.1 Excluded by Method 1.86 1 0.004654 0.04779 False High Sequest HT (A2) Unambiguous LSFSLK [K].lsFSLk.[T] N-Term(TMT6plex); S2(Phospho); K6(TMT6plex) 1 Q9NWB6 Q9NWB6 0 2 0.0721 0 1 1 616.85661 1232.70594 1232.7056 0.28 0.00017 3101239.25 CID (Collision Induced Dissociation) MS2 0.0 20 105.7 46.21 113.1074 37577 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 105.3 106.0 98.5 104.2 98.6 114.3 103.0 113.5 111.1 102.7 Excluded by Method 2.22 1 0.004654 0.04806 False High Sequest HT (A2) Unambiguous LSGFSFK [K].lSGFsFk.[K] N-Term(TMT6plex); S5(Phospho); K7(TMT6plex) 1 P29966 P29966 0 2 0.372 0 1 1 662.35946 1323.71164 1323.71141 0.17 0.00011 2107749.0 CID (Collision Induced Dissociation) MS2 11.36559 10 54.1 60.0 115.8905 38597 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 34.4 158.5 31.6 68.9 31.8 38.9 44.0 34.0 46.7 51.7 Excluded by Method 2.5 1 0.004654 0.04754 False High Sequest HT (A2) Unambiguous QLSPEDSSSPVK [K].qLsPEDSSSPVk.[S] N-Term(TMT6plex); S3(Phospho); K12(TMT6plex) 2 Q96AY2 Q96AY2-2; Q96AY2 0 3 0.3894 0 1 1 604.64463 1811.91933 1811.91923 0.05 3e-05 1531214.75 CID (Collision Induced Dissociation) MS2 6.005631 60 20.7 60.0 68.1719 20790 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 15.7 19.2 22.1 16.6 19.1 28.3 21.6 19.8 25.2 19.4 Excluded by Method 2.08 1 0.004654 0.04756 False High Sequest HT (A2) Unambiguous EDEENGPEEQAMSDEEELK [K].eDEENGPEEQAmsDEEELk.[Q] N-Term(TMT6plex); M12(Oxidation); S13(Phospho); K19(TMT6plex) 1 Q12019 Q12019 0 3 0.2696 0 1 1 921.39045 2762.15679 2762.16397 -2.6 -0.00239 152305.515625 CID (Collision Induced Dissociation) MS2 73.06925 20 18.3 60.0 77.2074 24198 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 13.5 18.5 16.7 11.1 16.3 21.8 17.5 21.2 21.7 25.0 Excluded by Method 2.04 1 0.004654 0.04802 False High Sequest HT (A2) Unambiguous DMESPTK [K].dmEsPTk.[L] N-Term(TMT6plex); M2(Oxidation); S4(Phospho); K7(TMT6plex) 4 P27816 P27816; P27816-5; P27816-6; P27816-2 0 3 0.3913 0 1 1 454.55261 1361.64329 1361.64241 0.65 0.00029 151439.34375 CID (Collision Induced Dissociation) MS2 3.996987 60 46.8 60.0 36.4876 9050 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 49.7 49.9 39.1 58.6 44.8 36.5 54.1 47.2 43.2 44.4 Excluded by Method 1.61 1 0.004759 0.05044 False High Sequest HT (A2) Unambiguous VLEESSVK [K].vLEESsVk.[D] N-Term(TMT6plex); S6(Phospho); K8(TMT6plex) 1 P46949 P46949 0 2 0.0921 0 1 1 714.8918 1428.77633 1428.77513 0.84 0.0006 1745735.0 CID (Collision Induced Dissociation) MS2 15.23253 20 213.5 60.0 72.1621 22243 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 54.5 178.7 318.3 62.6 187.0 408.6 81.4 203.0 429.5 211.6 Excluded by Method 3.15 1 0.004759 0.05084 False High Sequest HT (A2) Unambiguous SSTPEEVK [K].sStPEEVk.[K] N-Term(TMT6plex); T3(Phospho); K8(TMT6plex) 1 P23528 P23528 0 2 0.0373 0 1 1 707.8654 1414.72353 1414.7231 0.31 0.00022 1085658.625 CID (Collision Induced Dissociation) MS2 23.34483 30 266.7 60.0 46.8857 12858 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 274.1 262.7 191.3 290.7 262.7 258.8 298.8 292.9 268.9 265.8 Excluded by Method 2.41 1 0.004759 0.04904 False High Sequest HT (A2) Unambiguous GFITEDL [K].gFITEDL.[-] N-Term(TMT6plex) 1 P05738 P05738 0 2 0.6588 0 1 1 512.28163 1023.55597 1023.55599 -0.02 -1e-05 796545.25 CID (Collision Induced Dissociation) MS2 10.8704 50 248.3 60.0 109.5356 36310 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 56.8 194.6 418.0 74.2 182.8 508.3 79.3 216.7 494.8 257.5 Excluded by Method 1.7 1 0.004759 0.04961 False High Sequest HT (A2) Unambiguous DMESPTK [K].dmEsPTk.[L] N-Term(TMT6plex); M2(Oxidation); S4(Phospho); K7(TMT6plex) 4 P27816 P27816; P27816-5; P27816-6; P27816-2 0 2 0.0291 0 1 1 681.3249 1361.64253 1361.64241 0.09 6e-05 3416303.5 CID (Collision Induced Dissociation) MS2 2.418515 30 311.3 60.0 36.4361 9082 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 318.1 293.8 234.9 345.1 304.6 300.8 360.8 329.6 321.0 304.1 Excluded by Method 1.72 1 0.004759 0.04992 False High Sequest HT (A2) Unambiguous VRYPPSPAK [K].vRYPPsPAk.[M] N-Term(TMT6plex); S6(Phospho); K9(TMT6plex) 1 P30838 P30838 1 3 0.4145 0 1 1 518.29362 1552.8663 1552.86529 0.65 0.00034 390313.0 CID (Collision Induced Dissociation) MS2 0.0 50 83.3 60.0 58.2267 17007 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 82.0 85.3 55.9 87.8 80.7 80.9 94.2 94.3 86.2 85.5 Excluded by Method 1.52 1 0.004759 0.0512 False High Sequest HT (A2) Unambiguous ETERASPIK [K].eTERAsPIk.[M] N-Term(TMT6plex); S6(Phospho); K9(TMT6plex) 3 P27816 P27816; P27816-6; P27816-2 1 3 0.3184 0 1 1 523.62002 1568.84552 1568.84494 0.36 0.00019 2148333.5 CID (Collision Induced Dissociation) MS2 6.785692 30 275.7 60.0 44.2686 11862 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 280.6 271.2 222.4 290.5 259.3 268.0 312.0 279.3 281.1 292.8 Excluded by Method 1.79 1 0.004759 0.05097 False High Sequest HT (A2) Unambiguous RASFAK [K].rAsFAk.[S] N-Term(TMT6plex); S3(Phospho); K6(TMT6plex) 12 Q9H4A3 Q9H4A3-5; Q9Y3S1; Q9H4A3-2; Q9Y3S1-2; Q96J92; Q9H4A3-7; Q9H4A3; Q96J92-2; Q96J92-3; Q9Y3S1-4; Q9H4A3-6; Q9Y3S1-3 1 3 0.1108 0 1 1 406.56488 1217.68008 1217.68078 -0.57 -0.00023 5079712.5 CID (Collision Induced Dissociation) MS2 2.653838 30 214.7 59.187 47.7332 13171 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 220.5 205.1 166.9 218.0 202.1 223.2 235.7 230.9 203.2 241.5 Excluded by Method 3.34 1 0.004759 0.04905 False High Sequest HT (A2) Unambiguous EEQVSIK [K].eEQVsIk.[Q] N-Term(TMT6plex); S5(Phospho); K7(TMT6plex) 1 P32457 P32457 0 2 0.1581 0 1 1 685.87036 1370.73344 1370.73327 0.12 8e-05 3325617.25 CID (Collision Induced Dissociation) MS2 0.0 10 47.7 54.082 62.9243 18788 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 28.8 38.0 56.2 24.5 37.8 82.8 29.4 49.7 76.2 53.4 Excluded by Method 2.34 1 0.004759 0.05074 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVssDEEF.[-] N-Term(TMT6plex); S8(Phospho); S9(Phospho) 1 P16521 P16521 0 2 0.2388 0 1 1 925.35486 1849.70244 1849.70195 0.26 0.00024 2712827.0 CID (Collision Induced Dissociation) MS2 39.53916 60 26.1 23.316 127.9687 43197 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 8.5 22.9 39.5 7.3 26.5 50.6 7.2 22.6 53.5 22.4 Excluded by Method 2.01 1 0.004759 0.05118 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVSsDEEF.[-] N-Term(TMT6plex); S9(Phospho) 1 P16521 P16521 0 2 0.0429 0 1 1 885.37144 1769.73561 1769.73562 -0.01 -1e-05 129000.4296875 CID (Collision Induced Dissociation) MS2 40.38428 50 17.4 60.0 148.9171 50832 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 3.8 14.6 32.6 6.8 16.6 30.7 3.9 13.2 32.6 19.6 Excluded by Method 1.4 1 0.004759 0.05007 False High Sequest HT (A2) Unambiguous EKPLNSDQEDVFISEK [K].ekPLNsDQEDVFISEk.[T] N-Term(TMT6plex); K2(TMT6plex); S6(Phospho); K16(TMT6plex) 1 Q04279 Q04279 0 4 0.1325 0 1 1 662.09749 2645.36815 2645.36784 0.12 8e-05 769818.75 CID (Collision Induced Dissociation) MS2 40.90404 30 32.4 60.0 96.7159 31440 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 14.5 24.0 40.9 13.5 29.5 57.0 22.2 26.4 60.2 36.1 Excluded by Method 2.34 1 0.004759 0.04922 False High Sequest HT (A2) Unambiguous VVDYSQFQESDDADEDYGRDSGPPTK [K].vVDYSQFQEsDDADEDYGRDSGPPTk.[K] N-Term(TMT6plex); S10(Phospho); K26(TMT6plex) 1 Q9H1E3 Q9H1E3 1 4 0.1321 0 1 1 865.38788 3458.52968 3458.53134 -0.48 -0.00041 61685.91015625 CID (Collision Induced Dissociation) MS2 0.0 40 2.8 60.0 142.1366 48497 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 2.7 2.3 4.1 2.2 2.5 2.0 6.6 2.9 3.1 Excluded by Method 2.12 1 0.004759 0.05045 False High Sequest HT (A2) Unambiguous ELEAERSLMSSPEDLTK [K].eLEAERsLmsSPEDLTk.[D] N-Term(TMT6plex); S7(Phospho); M9(Oxidation); S10(Phospho); K17(TMT6plex) 1 P46821 P46821 1 3 0.0302 0 1 1 857.0687 2569.19154 2569.19099 0.21 0.00018 917221.875 CID (Collision Induced Dissociation) MS2 49.86185 20 53.2 60.0 92.4249 29835 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 48.4 53.2 50.5 54.2 54.9 52.6 63.6 49.6 56.0 48.6 Excluded by Method 2.32 1 0.004759 0.04991 False High Sequest HT (A2) Unambiguous SRSNLSQENDNEGK [K].sRSNLSQENDNEGk.[H] N-Term(TMT6plex); S1(Phospho); K14(TMT6plex) 1 P39705 P39705 1 3 0.0859 0 1 1 706.00717 2116.00696 2116.00721 -0.12 -8e-05 558314.625 CID (Collision Induced Dissociation) MS2 38.21164 30 12.5 60.0 38.4336 9808 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 2.8 11.7 18.8 2.7 8.7 23.0 6.6 12.5 25.1 13.5 Excluded by Method 1.98 1 0.004759 0.04915 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVssDEEF.[-] N-Term(TMT6plex); S8(Phospho); S9(Phospho) 1 P16521 P16521 0 2 0.1898 0 1 1 925.35501 1849.70275 1849.70195 0.43 0.0004 536827.3125 CID (Collision Induced Dissociation) MS2 12.43707 30 56.3 60.0 140.3927 47928 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 13.4 42.9 89.9 16.2 46.3 107.7 16.1 55.2 120.7 54.8 Excluded by Method 2.16 1 0.004759 0.04954 False High Sequest HT (A2) Unambiguous LDESDENEEALAK [K].lDEsDENEEALAk.[A] N-Term(TMT6plex); S4(Phospho); K13(TMT6plex) 1 Q08492 Q08492 0 2 0.557 0 1 1 1000.97652 2000.94576 2000.94657 -0.4 -0.0004 472479.6875 CID (Collision Induced Dissociation) MS2 0.0 20 12.0 60.0 82.7022 26178 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 1.9 10.5 15.1 6.5 10.4 21.4 3.8 14.1 22.7 13.1 Excluded by Method 3.07 1 0.004759 0.04912 False High Sequest HT (A2) Unambiguous AAAYDISEDEED [K].aAAYDIsEDEED.[-] N-Term(TMT6plex); S7(Phospho) 1 P49585 P49585 0 2 0.3357 0 1 1 818.82634 1636.64539 1636.64647 -0.66 -0.00054 261038.828125 CID (Collision Induced Dissociation) MS2 24.17241 40 45.2 60.0 79.4459 24957 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 43.1 42.8 33.3 47.2 46.9 44.6 46.8 58.5 44.1 44.5 Excluded by Method 2.83 1 0.004759 0.04921 False High Sequest HT (A2) Unambiguous TNLSGRQSPSFK [K].tNLSGRQsPSFk.[L] N-Term(TMT6plex); S8(Phospho); K12(TMT6plex) 3 Q6IN85 Q6IN85-2; Q6IN85; Q6IN85-4 1 3 0.1762 0 1 1 620.6639 1859.97715 1859.97808 -0.5 -0.00031 4363620.0 CID (Collision Induced Dissociation) MS2 13.97147 20 131.6 58.037 60.6135 17924 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 125.8 125.4 102.6 146.7 125.5 134.1 147.0 137.6 139.8 131.5 Excluded by Method 2.61 1 0.004759 0.04897 False High Sequest HT (A2) Unambiguous LTEGSD [R].lTEGSD.[-] N-Term(TMT6plex) 1 Q96MW7 Q96MW7 0 2 0.4211 0 1 1 425.72129 850.43531 850.43554 -0.28 -0.00012 56830.33203125 CID (Collision Induced Dissociation) MS2 0.0 0 1.7 60.0 11.508 1879 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 1.8 2.2 2.7 1.6 1.5 2.0 2.2 3.2 Excluded by Method 0.19 1 0.004759 0.0506 False High Sequest HT (A2) Unambiguous WFFISGSV [K].wFFISGsV.[-] N-Term(TMT6plex); S7(Phospho) 1 P00817 P00817 0 2 0.2488 0 1 1 626.30403 1251.60079 1251.60124 -0.37 -0.00023 177341.75 CID (Collision Induced Dissociation) MS2 21.7189 50 28.4 60.0 139.3706 47542 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 9.6 25.5 37.6 7.8 19.5 55.9 12.7 31.2 55.1 29.4 Excluded by Method 2.01 1 0.004759 0.04914 False High Sequest HT (A2) Unambiguous LASSGEVDK [K].lAsSGEVDk.[I] N-Term(TMT6plex); S3(Phospho); K9(TMT6plex) 1 P23201 P23201 0 2 0.0969 0 1 1 722.37977 1443.75226 1443.74965 1.81 0.00131 725627.125 CID (Collision Induced Dissociation) MS2 0.0 30 45.4 60.0 64.9392 19538 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 10.6 37.1 60.5 21.1 35.9 77.4 18.8 49.9 94.2 48.6 Excluded by Method 3.2 1 0.004888 0.05181 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVSsDEEF.[-] N-Term(TMT6plex); S9(Phospho) 1 P16521 P16521 0 2 0.0193 0 1 1 885.37071 1769.73414 1769.73562 -0.84 -0.00074 308937.59375 CID (Collision Induced Dissociation) MS2 35.65741 40 26.9 60.0 144.0528 49160 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 3.3 23.7 40.9 4.0 19.7 56.8 7.4 22.6 58.2 31.9 Excluded by Method 2.07 1 0.004888 0.05195 False High Sequest HT (A2) Unambiguous DMESPTK [K].dMEsPTk.[L] N-Term(TMT6plex); S4(Phospho); K7(TMT6plex) 4 P27816 P27816; P27816-5; P27816-6; P27816-2 0 2 0.1344 0 1 1 673.32764 1345.648 1345.64749 0.38 0.00025 4580762.0 CID (Collision Induced Dissociation) MS2 14.17227 30 172.8 34.121 51.5675 14537 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 177.7 173.3 128.8 182.2 159.5 159.8 207.2 192.4 177.5 169.9 Excluded by Method 2.53 1 0.004888 0.05132 False High Sequest HT (A2) Unambiguous LDVTLAK [K].lDVtLAk.[D] N-Term(TMT6plex); T4(Phospho); K7(TMT6plex) 4 P27816 P27816; P27816-5; P27816-6; P27816-2 0 2 0.1739 0 1 1 649.3806 1297.75393 1297.75328 0.5 0.00033 1313209.25 CID (Collision Induced Dissociation) MS2 34.1673 20 59.1 60.0 98.0909 32052 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 50.8 52.3 54.6 54.5 61.5 59.3 59.7 66.7 70.8 60.4 Excluded by Method 2.53 1 0.005141 0.05312 False High Sequest HT (A2) Unambiguous ISIGNK [KR].isIGNk.[SA] N-Term(TMT6plex); S2(Phospho); K6(TMT6plex) 1 Q05050 Q05050 0 2 0.0 0 1 1 585.33845 1169.66962 1169.66955 0.06 4e-05 23236304.0 CID (Collision Induced Dissociation) MS2 0.0 10 168.4 12.126 76.5265 23889 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 35.6 135.5 242.6 55.3 144.9 329.2 62.0 147.3 361.7 169.9 Excluded by Method 2.2 1 0.005141 0.05419 False High Sequest HT (A2) Unambiguous SIPEEQREQSPNK [K].sIPEEQREQsPNk.[M] N-Term(TMT6plex); S10(Phospho); K13(TMT6plex) 1 P40563 P40563 1 3 0.5127 0 1 1 694.02053 2080.04704 2080.04762 -0.28 -0.00019 546487.3125 CID (Collision Induced Dissociation) MS2 61.84299 60 79.0 60.0 49.4776 13770 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 22.4 62.9 124.4 27.0 67.3 151.9 29.7 69.6 149.7 85.2 Excluded by Method 3.16 1 0.005141 0.0523 False High Sequest HT (A2) Unambiguous VFDDESDEK [K].vFDDEsDEk.[E] N-Term(TMT6plex); S6(Phospho); K9(TMT6plex) 1 O43719 O43719 0 2 0.2452 0 1 1 811.37347 1621.73966 1621.73987 -0.13 -0.00011 286770.90625 CID (Collision Induced Dissociation) MS2 48.05799 40 86.2 60.0 77.893 24372 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 77.5 68.7 94.9 65.5 85.5 136.3 53.6 70.6 145.5 64.1 Excluded by Method 2.61 1 0.005141 0.05303 False High Sequest HT (A2) Unambiguous QGPSESPSPTK [K].qGPSESPsPTk.[V] N-Term(TMT6plex); S8(Phospho); K11(TMT6plex) 1 Q05040 Q05040 0 3 0.3333 0 1 1 551.61465 1652.82941 1652.82969 -0.17 -9e-05 422677.375 CID (Collision Induced Dissociation) MS2 12.01252 50 58.2 60.0 44.3076 11919 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 38.5 49.9 63.4 42.3 58.1 82.5 46.7 53.6 90.9 56.0 Excluded by Method 1.29 1 0.005141 0.05305 False High Sequest HT (A2) Unambiguous STSPSAAGGQQK [K].sTsPSAAGGQQk.[T] N-Term(TMT6plex); S3(Phospho); K12(TMT6plex) 1 Q2TB10 Q2TB10 0 3 0.0704 0 1 1 552.95049 1656.83692 1656.83584 0.66 0.00036 78108.8046875 CID (Collision Induced Dissociation) MS2 51.90455 70 5.3 60.0 37.2141 9367 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 1.8 4.2 2.2 7.8 6.6 4.2 7.2 8.2 7.0 4.1 1.42 1 0.005141 0.05239 False High Sequest HT (A2) Unambiguous ALVLELDEMSNGSEIQDALEEISGQK [K].aLVLELDEMSNGsEIQDALEEISGQk.[T] N-Term(TMT6plex); S13(Phospho); K26(TMT6plex) 2 P17695 P17695; P17695-2 0 4 0.0628 0 1 1 839.91962 3356.65665 3356.65845 -0.54 -0.00045 256260.40625 CID (Collision Induced Dissociation) MS2 59.5804 30 12.5 60.0 142.2083 48562 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 3.3 11.5 20.0 6.0 11.8 25.7 6.0 10.1 19.9 10.5 Excluded by Method 1.91 1 0.005141 0.05244 False High Sequest HT (A2) Unambiguous IGIVEISPK [K].iGIVEIsPk.[G] N-Term(TMT6plex); S7(Phospho); K9(TMT6plex) 2 P07280 P07280; P07281 0 2 0.1991 0 1 1 747.44103 1493.87478 1493.87445 0.22 0.00016 978730.9375 CID (Collision Induced Dissociation) MS2 37.65658 20 47.6 60.0 108.0866 35704 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 14.9 37.6 67.5 19.1 44.9 89.5 16.0 40.3 92.2 53.7 Excluded by Method 2.16 1 0.005141 0.05254 False High Sequest HT (A2) Unambiguous GTVQQADE [K].gTVQQADE.[-] N-Term(TMT6plex) 1 P32969 P32969 0 2 0.5369 0 1 1 538.77446 1076.54164 1076.54213 -0.46 -0.00025 126648.1171875 CID (Collision Induced Dissociation) MS2 12.41015 90 9.5 60.0 29.3124 6567 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 10.4 8.0 9.3 9.2 9.1 11.4 12.3 7.0 10.2 8.1 1.49 1 0.005259 0.05431 False High Sequest HT (A2) Unambiguous TSTSPPPEK [K].tSTsPPPEk.[S] N-Term(TMT6plex); S4(Phospho); K9(TMT6plex) 2 Q9H1E3 Q9H1E3; Q9H1E3-2 0 3 0.0231 0 1 1 494.59365 1481.76639 1481.7653 0.74 0.00036 159183.515625 CID (Collision Induced Dissociation) MS2 37.19917 40 407.2 60.0 44.739 12033 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 417.1 414.6 354.2 430.7 368.4 390.5 465.6 425.3 406.0 399.3 Excluded by Method 2.16 1 0.005259 0.05564 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVssDEEF.[-] N-Term(TMT6plex); S8(Phospho); S9(Phospho) 1 P16521 P16521 0 2 0.1841 0 1 1 925.35489 1849.7025 1849.70195 0.3 0.00027 5869340.5 CID (Collision Induced Dissociation) MS2 40.09845 50 67.5 11.317 127.891 43086 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 20.1 53.6 104.9 25.1 51.9 127.2 28.1 64.6 136.9 63.1 Excluded by Method 2.39 1 0.005259 0.05456 False High Sequest HT (A2) Unambiguous HDGGSPSIK [K].hDGGsPSIk.[A] N-Term(TMT6plex); S5(Phospho); K9(TMT6plex) 1 Q15648 Q15648 0 3 0.4367 0 1 1 479.2494 1435.73366 1435.73467 -0.7 -0.00034 507595.96875 CID (Collision Induced Dissociation) MS2 3.058991 40 65.8 60.0 40.0185 10375 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 63.9 64.2 56.9 71.4 66.6 65.5 67.4 64.8 68.3 69.1 Excluded by Method 2.45 1 0.005259 0.0545 False High Sequest HT (A2) Unambiguous DSTLIMQLLRDNLTLWTSENQGDEGDAGEGEN [K].dSTLImQLLRDNLTLWTSENQGDEGDAGEGEN.[-] N-Term(TMT6plex); M6(Oxidation) 2 P31946 P31946-2; P31946 1 4 0.217 0 1 1 942.44606 3766.76242 3766.75512 1.94 0.00183 11389732.0 CID (Collision Induced Dissociation) MS2 2.978917 80 10.1 5.7 128.889 43580 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 9.8 10.9 7.8 7.6 10.5 8.5 11.1 11.4 12.9 10.3 3.41 1 0.005259 0.05555 False High Sequest HT (A2) Unambiguous EEEEAKEESDDDMGFGLFD [K].eEEEAkEEsDDDmGFGLFD.[-] N-Term(TMT6plex); K6(TMT6plex); S9(Phospho); M13(Oxidation) 1 P05318 P05318 1 3 0.5472 0 1 1 916.0512 2746.13905 2746.13669 0.86 0.00079 1639442.75 CID (Collision Induced Dissociation) MS2 8.520776 50 127.6 44.107 116.5031 38857 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 31.5 98.7 185.6 44.0 102.8 237.7 48.0 114.7 285.5 127.6 Excluded by Method 3.07 1 0.005373 0.05738 False High Sequest HT (A2) Unambiguous DGQTDSAAEESSSQETSK [K].dGQTDsAAEESSSQETSk.[GD] N-Term(TMT6plex); S6(Phospho); K18(TMT6plex) 4 P55201 P55201-3; P55201-4; P55201-2; P55201 0 3 0.1184 0 1 1 799.02388 2395.05708 2395.05501 0.86 0.00069 701607.5 CID (Collision Induced Dissociation) MS2 0.0 70 5.0 60.0 43.9289 11782 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 3.4 4.0 2.2 3.7 7.3 6.5 11.1 3.2 2.7 5.4 1.52 1 0.005373 0.0562 False High Sequest HT (A2) Unambiguous LSGFSFK [K].lSGFsFk.[K] N-Term(TMT6plex); S5(Phospho); K7(TMT6plex) 1 P29966 P29966 0 2 0.3744 0 1 1 662.35941 1323.71154 1323.71141 0.1 7e-05 425604.21875 CID (Collision Induced Dissociation) MS2 20.4424 10 19.7 60.0 121.5008 40754 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 15.2 21.8 17.1 15.9 18.1 23.3 18.7 21.9 25.3 20.1 Excluded by Method 2.11 1 0.005373 0.05731 False High Sequest HT (A2) Unambiguous RGSVRGTSAADV [K].rGsVRGTSAADV.[-] N-Term(TMT6plex); S3(Phospho) 2 P48739 P48739; P48739-3 2 3 0.216 0 1 1 495.5859 1484.74316 1484.74199 0.79 0.00039 180698.484375 CID (Collision Induced Dissociation) MS2 5.268599 20 268.0 60.0 35.3718 8701 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 273.0 264.2 230.8 282.0 257.7 262.5 304.6 296.9 271.5 237.1 Excluded by Method 1.62 1 0.005373 0.05781 False High Sequest HT (A2) Unambiguous STLESEKPGSPEAAETSPPSNIIDHCEK [K].sTLESEkPGsPEAAETsPPSNIIDHcEk.[L] N-Term(TMT6plex); K7(TMT6plex); S10(Phospho); S17(Phospho); C26(Carbamidomethyl); K28(TMT6plex) 3 Q96T23 Q96T23; Q96T23-3; Q96T23-2 0 4 0.123 0 1 1 965.20742 3857.80784 3857.81606 -2.13 -0.00205 683318.9375 CID (Collision Induced Dissociation) MS2 0.0 20 84.4 60.0 88.3047 28288 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 92.8 83.6 71.4 94.5 82.2 74.4 91.5 91.9 74.7 87.2 Excluded by Method 4.47 1 0.005373 0.05643 False High Sequest HT (A2) Unambiguous DMSPLSETEMALGK [K].dMsPLSETEmALGk.[D] N-Term(TMT6plex); S3(Phospho); M10(Oxidation); K14(TMT6plex) 3 P27816 P27816; P27816-6; P27816-2 0 3 0.0048 0 1 1 688.33361 2062.98628 2062.98422 1.0 0.00069 493205.0625 CID (Collision Induced Dissociation) MS2 44.67617 50 66.1 60.0 121.1824 40584 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 57.5 67.4 70.2 48.0 59.0 90.8 55.7 58.6 87.9 66.2 Excluded by Method 2.1 1 0.005373 0.05638 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVsSDEEF.[-] N-Term(TMT6plex); S8(Phospho) 1 P16521 P16521 0 2 0.4378 0 1 1 885.37297 1769.73866 1769.73562 1.72 0.00152 392625.375 CID (Collision Induced Dissociation) MS2 34.6227 40 48.7 60.0 134.5125 45874 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 22.2 42.3 65.2 25.8 35.7 88.4 22.8 51.4 85.8 47.2 Excluded by Method 2.01 1 0.005373 0.05779 False High Sequest HT (A2) Unambiguous ELEAERSLMSSPEDLTK [K].eLEAERsLmsSPEDLTk.[D] N-Term(TMT6plex); S7(Phospho); M9(Oxidation); S10(Phospho); K17(TMT6plex) 1 P46821 P46821 1 3 0.2694 0 1 1 857.06819 2569.19001 2569.19099 -0.38 -0.00033 476078.4375 CID (Collision Induced Dissociation) MS2 47.34386 10 63.5 60.0 89.038 28642 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 54.4 58.5 63.5 54.6 59.9 75.4 63.7 69.4 75.9 59.3 Excluded by Method 2.45 1 0.005373 0.05756 False High Sequest HT (A2) Unambiguous WFFISGSV [K].wFFISGsV.[-] N-Term(TMT6plex); S7(Phospho) 1 P00817 P00817 0 2 0.2118 0 1 1 626.30379 1251.6003 1251.60124 -0.76 -0.00047 157957.859375 CID (Collision Induced Dissociation) MS2 0.0 50 21.9 60.0 141.1238 48148 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 6.6 19.3 32.9 9.2 15.0 37.2 9.5 18.4 52.3 18.2 Excluded by Method 1.7 1 0.005373 0.05675 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVssDEEF.[-] N-Term(TMT6plex); S8(Phospho); S9(Phospho) 1 P16521 P16521 0 2 0.2637 0 1 1 925.35321 1849.69915 1849.70195 -1.52 -0.0014 605749.0625 CID (Collision Induced Dissociation) MS2 20.55628 40 38.8 60.0 133.6534 45568 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 8.9 31.0 61.0 11.9 24.4 71.5 15.3 43.1 79.1 42.1 Excluded by Method 1.82 1 0.0055 0.05788 False High Sequest HT (A2) Unambiguous EEEEAKEESDDDMGFGLFD [K].eEEEAkEEsDDDmGFGLFD.[-] N-Term(TMT6plex); K6(TMT6plex); S9(Phospho); M13(Oxidation) 1 P05318 P05318 1 3 0.474 0 1 1 916.05248 2746.1429 2746.13669 2.26 0.00207 553629.4375 CID (Collision Induced Dissociation) MS2 13.05569 50 146.4 60.0 112.914 37541 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 34.3 117.5 221.2 56.2 126.8 249.3 49.1 146.3 306.3 157.0 Excluded by Method 3.46 1 0.005622 0.05937 False High Sequest HT (A2) Unambiguous DISTPDLSK [K].dIsTPDLSk.[V] N-Term(TMT6plex); S3(Phospho); K9(TMT6plex) 1 Q12675 Q12675 0 3 0.0311 0 1 1 505.26882 1513.79192 1513.79151 0.27 0.00014 1203776.375 CID (Collision Induced Dissociation) MS2 0.0 20 49.7 60.0 82.864 26275 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 13.4 41.2 68.4 19.2 42.3 91.8 21.8 44.7 102.0 52.2 Excluded by Method 2.89 1 0.005622 0.05931 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVsSDEEF.[-] N-Term(TMT6plex); S8(Phospho) 1 P16521 P16521 0 2 0.0045 0 1 1 885.37309 1769.7389 1769.73562 1.85 0.00164 222540.4375 CID (Collision Induced Dissociation) MS2 22.979 50 33.7 60.0 140.7595 48023 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 10.4 25.2 53.3 11.9 31.1 63.5 12.6 34.2 68.9 26.0 Excluded by Method 2.24 1 0.005622 0.05983 False High Sequest HT (A2) Unambiguous EPLIPASPK [K].ePLIPAsPk.[A] N-Term(TMT6plex); S7(Phospho); K9(TMT6plex) 1 Q9Y613 Q9Y613 0 3 0.5992 0 1 1 497.28566 1489.84242 1489.84315 -0.49 -0.00024 791004.5 CID (Collision Induced Dissociation) MS2 0.0 30 87.3 60.0 86.4625 27594 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 82.5 94.3 72.0 92.7 88.5 84.7 99.4 94.1 83.9 80.9 Excluded by Method 2.42 1 0.006008 0.06095 False High Sequest HT (A2) Unambiguous EECPRPMSISPPDFSPK [K].eEcPRPmSISPPDFsPk.[T] N-Term(TMT6plex); C3(Carbamidomethyl); M7(Oxidation); S15(Phospho); K17(TMT6plex) 1 P46821 P46821 0 3 0.181 0 1 1 843.40301 2528.19448 2528.19667 -0.86 -0.00073 425727.65625 CID (Collision Induced Dissociation) MS2 18.19601 30 45.2 60.0 85.3571 27181 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 42.7 46.9 33.5 45.0 48.9 44.7 42.8 47.3 55.3 44.6 Excluded by Method 2.1 1 0.006233 0.06448 False High Sequest HT (A2) Unambiguous LSGFSFK [K].lSGFsFk.[K] N-Term(TMT6plex); S5(Phospho); K7(TMT6plex) 1 P29966 P29966 0 2 0.428 0 1 1 662.35947 1323.71166 1323.71141 0.19 0.00013 6466314.5 CID (Collision Induced Dissociation) MS2 0.0 10 141.9 43.005 113.2924 37716 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 152.1 143.7 114.7 143.1 135.4 136.2 150.8 157.9 144.8 140.7 Excluded by Method 2.5 1 0.006233 0.06449 False High Sequest HT (A2) Unambiguous GPAESPDEGITTTEGEGECEQTPEELEPVEK [K].gPAESPDEGITTTEGEGEcEQtPEELEPVEk.[Q] N-Term(TMT6plex); C19(Carbamidomethyl); T22(Phospho); K31(TMT6plex) 1 P46821 P46821 0 4 0.0374 0 1 1 971.43992 3882.73786 3882.74041 -0.66 -0.00064 790969.375 CID (Collision Induced Dissociation) MS2 0.0 50 8.4 60.0 94.7002 30718 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 9.2 4.2 8.3 3.9 9.8 12.7 11.0 8.3 10.6 6.2 Excluded by Method 2.14 1 0.006233 0.06374 False High Sequest HT (A2) Unambiguous DVPPLSETEATPVPIK [K].dVPPLSETEAtPVPIk.[D] N-Term(TMT6plex); T11(Phospho); K16(TMT6plex) 2 P27816 P27816; P27816-6 0 3 0.0323 0 1 1 744.4036 2231.19626 2231.19764 -0.62 -0.00046 744974.3125 CID (Collision Induced Dissociation) MS2 40.70801 20 58.6 47.995 104.8087 34516 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 48.3 47.7 62.2 53.4 60.8 73.0 56.4 70.4 65.2 48.6 Excluded by Method 1.86 1 0.006233 0.06563 False High Sequest HT (A2) Unambiguous LASSGEVDK [K].lAsSGEVDk.[I] N-Term(TMT6plex); S3(Phospho); K9(TMT6plex) 1 P23201 P23201 0 2 0.1454 0 1 1 722.37845 1443.74962 1443.74965 -0.02 -1e-05 1578856.375 CID (Collision Induced Dissociation) MS2 5.787312 10 41.6 60.0 65.6253 19829 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 22.8 34.9 47.6 24.3 34.5 63.6 29.4 40.5 76.7 42.0 Excluded by Method 2.27 1 0.006233 0.0621 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVssDEEF.[-] N-Term(TMT6plex); S8(Phospho); S9(Phospho) 1 P16521 P16521 0 2 0.1085 0 1 1 925.35587 1849.70446 1849.70195 1.35 0.00125 758174.375 CID (Collision Induced Dissociation) MS2 14.15402 30 72.7 60.0 113.3322 37659 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 20.3 61.9 118.5 20.5 58.8 137.1 27.6 66.9 139.4 76.4 Excluded by Method 2.58 1 0.006233 0.06529 False High Sequest HT (A2) Unambiguous EEEAKEESDDDMGFGLFD [K].eEEAkEEsDDDmGFGLFD.[-] N-Term(TMT6plex); K5(TMT6plex); S8(Phospho); M12(Oxidation) 1 P02400 P02400 1 3 0.523 0 1 1 873.03833 2617.10042 2617.0941 2.42 0.00211 963793.3125 CID (Collision Induced Dissociation) MS2 0.0 20 111.6 60.0 125.4723 42202 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 24.7 86.2 166.8 40.1 92.0 198.7 41.4 122.6 232.9 110.9 Excluded by Method 3.04 1 0.006233 0.06481 False High Sequest HT (A2) Unambiguous VGILPSSSTDDVTSK [K].vGILPSSStDDVTSk.[E] N-Term(TMT6plex); T9(Phospho); K15(TMT6plex) 1 P22215 P22215 0 2 0.0052 0 1 1 1022.53446 2044.06164 2044.06154 0.05 5e-05 422893.34375 CID (Collision Induced Dissociation) MS2 0.0 50 25.9 60.0 98.045 31978 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 12.6 22.5 33.8 15.8 24.6 38.0 13.3 26.6 41.8 29.9 Excluded by Method 1.93 1 0.006233 0.06205 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVssDEEF.[-] N-Term(TMT6plex); S8(Phospho); S9(Phospho) 1 P16521 P16521 0 2 0.2424 0 1 1 925.35368 1849.70008 1849.70195 -1.01 -0.00094 957572.9375 CID (Collision Induced Dissociation) MS2 0.0 50 26.1 43.179 130.549 44313 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 8.1 22.8 43.8 4.0 23.3 47.0 10.9 25.1 47.5 28.1 Excluded by Method 1.98 1 0.006233 0.06243 False High Sequest HT (A2) Unambiguous EEEAAEESDDDMGFGLFD [K].eEEAAEEsDDDmGFGLFD.[-] N-Term(TMT6plex); S8(Phospho); M12(Oxidation) 2 P05319 P10622; P05319 0 3 0.6338 0 1 1 777.6336 2330.88625 2330.87332 5.55 0.00431 1818485.375 CID (Collision Induced Dissociation) MS2 4.981381 50 79.0 43.835 141.5072 48279 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 13.2 63.5 132.8 27.2 61.6 140.4 29.5 69.8 166.8 85.6 Excluded by Method 3.25 1 0.006233 0.06578 False High Sequest HT (A2) Unambiguous TVAELMEN [K].tVAELmEN.[-] N-Term(TMT6plex); M6(Oxidation) 1 P53981 P53981 0 2 0.4144 0 1 1 576.29444 1151.5816 1151.58156 0.03 2e-05 990121.1875 CID (Collision Induced Dissociation) MS2 16.94059 90 139.6 60.0 61.3981 18253 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 28.8 108.7 229.9 43.3 112.2 274.4 41.2 117.1 284.3 155.7 2.22 1 0.006233 0.06531 False High Sequest HT (A2) Unambiguous QGGSPDEPDSK [K].qGGsPDEPDSk.[A] N-Term(TMT6plex); S4(Phospho); K11(TMT6plex) 2 Q9ULX6 Q9ULX6; Q9ULX6-2 0 2 0.5194 0 1 1 827.8903 1654.77333 1654.77257 0.46 0.00038 237649.9375 CID (Collision Induced Dissociation) MS2 0.0 40 11.6 60.0 39.7671 10283 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 11.3 9.2 11.0 9.3 11.6 13.5 11.5 11.5 15.3 11.4 Excluded by Method 2.06 1 0.006365 0.06598 False High Sequest HT (A2) Unambiguous STLESEKPGSPEAAETSPPSNIIDHCEK [K].sTLESEkPGsPEAAETsPPSNIIDHcEk.[L] N-Term(TMT6plex); K7(TMT6plex); S10(Phospho); S17(Phospho); C26(Carbamidomethyl); K28(TMT6plex) 3 Q96T23 Q96T23; Q96T23-3; Q96T23-2 0 4 0.0642 0 1 1 965.20778 3857.8093 3857.81606 -1.75 -0.00169 542457.8125 CID (Collision Induced Dissociation) MS2 0.0 30 76.7 60.0 88.0226 28263 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 78.6 79.2 59.3 86.4 68.1 76.7 87.9 76.5 79.8 74.4 Excluded by Method 3.74 1 0.006451 0.07048 False High Sequest HT (A2) Unambiguous ESLSPGK [K].eSLsPGk.[L] N-Term(TMT6plex); S4(Phospho); K7(TMT6plex) 3 Q0ZGT2 Q0ZGT2-2; Q0ZGT2-4; Q0ZGT2 0 2 0.252 0 1 1 628.33846 1255.66964 1255.66994 -0.24 -0.00015 4114855.25 CID (Collision Induced Dissociation) MS2 0.0 30 88.1 60.0 55.1032 15847 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 86.3 91.6 69.1 92.3 91.1 86.5 89.8 88.5 91.8 93.7 Excluded by Method 2.54 1 0.006451 0.06655 False High Sequest HT (A2) Unambiguous HIVSNDSSDSDDESHEPK [K].hIVSNDSSDSDDEsHEPk.[G] N-Term(TMT6plex); S14(Phospho); K18(TMT6plex) 1 P47712 P47712 0 3 0.0264 0 1 1 846.04568 2536.12248 2536.12409 -0.64 -0.00054 409917.34375 CID (Collision Induced Dissociation) MS2 0.0 60 5.1 60.0 43.2752 11545 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 3.5 6.4 3.7 3.6 4.1 3.5 2.7 6.3 13.4 3.5 Excluded by Method 2.65 1 0.006451 0.07026 False High Sequest HT (A2) Unambiguous MEGSDFESSGGRGLK [K].mEGsDFESSGGRGLk.[K] N-Term(TMT6plex); M1(Oxidation); S4(Phospho); K15(TMT6plex) 2 Q8WYH8 Q8WYH8-2; Q8WYH8 1 3 0.3053 0 1 1 704.33399 2110.98742 2110.98806 -0.3 -0.00021 2024501.125 CID (Collision Induced Dissociation) MS2 0.0 10 125.4 60.0 62.0173 18426 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 118.0 126.1 98.3 117.7 119.9 133.3 127.1 144.1 133.5 135.7 Excluded by Method 2.26 1 0.006451 0.07009 False High Sequest HT (A2) Unambiguous EVPSKEEPSPVK [K].eVPSkEEPsPVk.[A] N-Term(TMT6plex); K5(TMT6plex); S9(Phospho); K12(TMT6plex) 1 P46821 P46821 1 4 0.166 0 1 1 524.0429 2093.14979 2093.14985 -0.03 -1e-05 1295720.125 CID (Collision Induced Dissociation) MS2 21.69099 20 59.2 59.328 64.5329 19428 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 53.4 62.7 49.5 54.8 60.8 63.9 61.8 57.1 66.7 61.0 Excluded by Method 2.35 1 0.006451 0.07005 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVssDEEF.[-] N-Term(TMT6plex); S8(Phospho); S9(Phospho) 1 P16521 P16521 0 2 0.1422 0 1 1 925.35474 1849.7022 1849.70195 0.13 0.00012 945772.0 CID (Collision Induced Dissociation) MS2 36.39814 40 27.6 60.0 131.6489 44806 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 10.5 27.2 44.0 7.7 24.8 50.6 13.5 26.1 48.3 22.9 Excluded by Method 2.25 1 0.006451 0.06967 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVSsDEEF.[-] N-Term(TMT6plex); S9(Phospho) 1 P16521 P16521 0 2 0.0533 0 1 1 885.37064 1769.734 1769.73562 -0.92 -0.00081 450434.34375 CID (Collision Induced Dissociation) MS2 25.30176 40 59.8 60.0 124.7419 41889 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 17.1 51.6 85.2 19.9 55.3 110.9 22.7 50.7 116.0 69.1 Excluded by Method 2.44 1 0.006451 0.06898 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVssDEEF.[-] N-Term(TMT6plex); S8(Phospho); S9(Phospho) 1 P16521 P16521 0 2 0.1435 0 1 1 925.35545 1849.70362 1849.70195 0.9 0.00083 621333.6875 CID (Collision Induced Dissociation) MS2 43.50769 30 60.2 60.0 145.6271 49706 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 19.0 49.7 97.9 19.0 46.7 109.6 23.8 54.9 119.9 61.0 Excluded by Method 2.23 1 0.006451 0.06794 False High Sequest HT (A2) Unambiguous VLSDSEDEEK [K].vLsDsEDEEk.[D] N-Term(TMT6plex); S3(Phospho); S5(Phospho); K10(TMT6plex) 1 Q8WWQ0 Q8WWQ0 0 2 0.3091 0 1 1 884.88743 1768.76759 1768.76953 -1.1 -0.00097 1038974.0 CID (Collision Induced Dissociation) MS2 28.32652 20 47.0 60.0 72.4376 22404 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 50.7 47.7 32.7 50.3 46.6 49.5 49.9 49.0 46.2 47.1 Excluded by Method 2.75 1 0.006451 0.06888 False High Sequest HT (A2) Unambiguous HEQPQIESPK [K].hEQPQIEsPk.[-] N-Term(TMT6plex); S8(Phospho); K10(TMT6plex) 1 P40558 P40558 0 3 0.2917 0 1 1 577.63445 1730.8888 1730.88787 0.54 0.00031 353788.59375 CID (Collision Induced Dissociation) MS2 43.07592 50 18.2 60.0 46.5458 12694 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 9.4 16.2 25.1 13.8 17.5 27.1 14.0 14.2 27.1 17.4 Excluded by Method 1.68 1 0.006451 0.06975 False High Sequest HT (A2) Unambiguous STFREESPLRIK [K].sTFREEsPLRIk.[M] N-Term(TMT6plex); S7(Phospho); K12(TMT6plex) 4 Q9NYF8 Q9NYF8-3; Q9NYF8-4; Q9NYF8; Q9NYF8-2 2 4 0.28 0 1 1 501.0286 2001.09258 2001.09345 -0.43 -0.00022 61481896.0 CID (Collision Induced Dissociation) MS2 0.0 0 177.6 4.495 74.6059 23131 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 171.2 164.8 139.0 158.4 171.0 187.8 204.2 189.1 182.5 208.0 Excluded by Method 2.0 1 0.006451 0.07133 False High Sequest HT (A2) Unambiguous HIQEEEEQDVDMTGDEEQEEEPDREK [K].hIQEEEEQDVDmtGDEEQEEEPDREk.[I] N-Term(TMT6plex); M12(Oxidation); T13(Phospho); K26(TMT6plex) 1 P28000 P28000 1 4 0.401 0 1 1 932.65154 3727.58434 3727.58424 0.03 3e-05 600919.375 CID (Collision Induced Dissociation) MS2 44.11687 70 1.6 60.0 55.342 15994 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 2.2 2.7 1.9 9.6 1.92 1 0.006573 0.07153 False High Sequest HT (A2) Unambiguous QGGSPDEPDSK [K].qGGsPDEPDSk.[A] N-Term(TMT6plex); S4(Phospho); K11(TMT6plex) 2 Q9ULX6 Q9ULX6; Q9ULX6-2 0 2 0.5458 0 1 1 827.88961 1654.77195 1654.77257 -0.37 -0.00031 76170.8125 CID (Collision Induced Dissociation) MS2 26.17302 60 29.5 60.0 39.8425 10262 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 29.0 35.0 22.0 27.9 28.8 29.0 32.4 32.5 33.2 24.7 Excluded by Method 2.73 1 0.006573 0.07169 False High Sequest HT (A2) Unambiguous EEQVSIK [K].eEQVsIk.[Q] N-Term(TMT6plex); S5(Phospho); K7(TMT6plex) 1 P32457 P32457 0 2 0.0457 0 1 1 685.87037 1370.73346 1370.73327 0.14 0.0001 2883243.0 CID (Collision Induced Dissociation) MS2 0.0 20 26.7 60.0 63.5764 19071 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 18.7 23.9 32.1 20.7 22.7 32.9 23.8 21.3 41.1 29.6 Excluded by Method 1.97 1 0.006573 0.07277 False High Sequest HT (A2) Unambiguous SPSLSPSPPSPLEK [K].sPSLSPsPPsPLEk.[T] N-Term(TMT6plex); S7(Phospho); S10(Phospho); K14(TMT6plex) 1 P46821 P46821 0 3 0.2379 0 1 1 681.00835 2041.01049 2041.00601 2.19 0.00149 438338.5625 CID (Collision Induced Dissociation) MS2 33.25174 20 96.3 60.0 93.3837 30191 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 99.3 91.8 76.8 108.6 96.4 98.0 101.2 102.2 97.4 91.3 Excluded by Method 4.12 1 0.006697 0.07285 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVsSDEEF.[-] N-Term(TMT6plex); S8(Phospho) 1 P16521 P16521 0 2 0.0058 0 1 1 885.3723 1769.73732 1769.73562 0.96 0.00085 313603.84375 CID (Collision Induced Dissociation) MS2 48.45224 50 47.9 60.0 136.5561 46584 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 11.8 38.3 74.8 16.0 34.7 87.6 19.4 48.8 94.1 53.1 Excluded by Method 1.71 1 0.006825 0.07359 False High Sequest HT (A2) Unambiguous EISTPTHWSK [K].eIStPTHWSk.[L] N-Term(TMT6plex); T4(Phospho); K10(TMT6plex) 2 Q8IX12 Q8IX12-2; Q8IX12 0 3 0.076 0 1 1 575.29877 1723.88176 1723.88206 -0.17 -0.0001 533936.875 CID (Collision Induced Dissociation) MS2 25.51134 40 67.5 60.0 77.7789 24414 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 66.6 55.9 60.8 56.2 66.7 73.9 71.3 78.1 73.0 72.9 Excluded by Method 1.71 1 0.006955 0.07421 False High Sequest HT (A2) Unambiguous SFEERVETTVTSLK [K].sFEERVETTVTSLk.[T] N-Term(TMT6plex); S1(Phospho); K14(TMT6plex) 2 Q16890 Q16890; Q16890-5 1 3 0.072 0 1 1 722.04892 2164.13222 2164.13029 0.89 0.00064 619013.8125 CID (Collision Induced Dissociation) MS2 25.27002 30 23.7 57.094 120.8119 40418 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 25.1 29.7 22.1 26.1 19.7 21.7 21.3 23.2 26.3 21.6 Excluded by Method 2.5 1 0.007334 0.07604 False High Sequest HT (A2) Unambiguous EEEAAEESDDDMGFGLFD [K].eEEAAEESDDDmGFGLFD.[-] N-Term(TMT6plex); M12(Oxidation) 2 P05319 P10622; P05319 0 2 0.3841 0 1 1 1125.95633 2250.90539 2250.90698 -0.71 -0.0008 310918.875 CID (Collision Induced Dissociation) MS2 0.0 60 8.6 60.0 115.1691 38333 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 2.5 9.6 9.4 5.9 6.5 15.4 6.3 9.5 12.7 8.2 Excluded by Method 1.38 1 0.007334 0.07564 False High Sequest HT (A2) Unambiguous LSGFSFK [K].lSGFsFk.[K] N-Term(TMT6plex); S5(Phospho); K7(TMT6plex) 1 P29966 P29966 0 2 0.3448 0 1 1 662.35935 1323.71142 1323.71141 0.01 0.0 138559.9375 CID (Collision Induced Dissociation) MS2 0.0 0 15.6 60.0 148.3543 50633 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 14.2 10.6 15.2 13.6 16.6 15.7 15.9 20.9 18.5 14.6 Excluded by Method 1.74 1 0.007334 0.07574 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVSsDEEF.[-] N-Term(TMT6plex); S9(Phospho) 1 P16521 P16521 0 2 0.0255 0 1 1 885.37413 1769.74098 1769.73562 3.03 0.00268 603028.4375 CID (Collision Induced Dissociation) MS2 33.06723 40 76.0 60.0 120.0002 40114 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 17.7 54.9 116.6 22.4 63.4 154.2 25.9 74.2 152.3 78.7 Excluded by Method 2.74 1 0.007334 0.07532 False High Sequest HT (A2) Unambiguous LSGFSFK [K].lSGFsFk.[K] N-Term(TMT6plex); S5(Phospho); K7(TMT6plex) 1 P29966 P29966 0 2 0.4417 0 1 1 662.35916 1323.71104 1323.71141 -0.28 -0.00019 264850.0 CID (Collision Induced Dissociation) MS2 0.0 10 22.6 60.0 121.5039 40705 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 23.6 18.5 20.8 24.2 19.3 29.3 25.4 22.7 23.9 18.3 Excluded by Method 2.06 1 0.007461 0.07752 False High Sequest HT (A2) Unambiguous EEEAKEESDDDMGFGLFD [K].eEEAkEEsDDDmGFGLFD.[-] N-Term(TMT6plex); K5(TMT6plex); S8(Phospho); M12(Oxidation) 1 P02400 P02400 1 3 0.5769 0 1 1 873.03838 2617.10059 2617.0941 2.48 0.00216 919820.125 CID (Collision Induced Dissociation) MS2 13.51143 40 89.1 60.0 125.5115 42173 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 27.5 73.0 136.8 38.2 73.0 158.0 43.5 89.6 165.9 85.7 Excluded by Method 2.34 1 0.007461 0.07748 False High Sequest HT (A2) Unambiguous GFITEDL [K].gFITEDL.[-] N-Term(TMT6plex) 1 P05738 P05738 0 2 0.4591 0 1 1 512.2816 1023.55593 1023.55599 -0.07 -3e-05 1999875.375 CID (Collision Induced Dissociation) MS2 0.0 50 312.7 60.0 109.1431 36129 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 73.1 252.0 530.1 93.3 237.5 621.8 109.7 290.1 615.7 304.1 Excluded by Method 1.59 1 0.00757 0.07947 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVSsDEEF.[-] N-Term(TMT6plex); S9(Phospho) 1 P16521 P16521 0 2 0.0047 0 1 1 885.37327 1769.73927 1769.73562 2.06 0.00182 280973.625 CID (Collision Induced Dissociation) MS2 0.0 50 24.3 60.0 147.0104 50232 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 7.4 15.9 37.2 7.5 17.0 46.2 7.2 25.3 53.7 25.1 Excluded by Method 2.14 1 0.00757 0.07818 False High Sequest HT (A2) Unambiguous LSGFSFK [K].lSGFsFk.[K] N-Term(TMT6plex); S5(Phospho); K7(TMT6plex) 1 P29966 P29966 0 2 0.4297 0 1 1 662.35941 1323.71154 1323.71141 0.1 7e-05 2038826.125 CID (Collision Induced Dissociation) MS2 0.0 10 61.8 60.0 114.5734 38191 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 58.2 61.1 50.6 71.7 51.6 55.7 70.4 73.1 65.4 60.5 Excluded by Method 2.49 1 0.00757 0.07875 False High Sequest HT (A2) Unambiguous KETQSPEQVK [K].kEtQSPEQVk.[S] N-Term(TMT6plex); K1(TMT6plex); T3(Phospho); K10(TMT6plex) 3 Q9NYF8 Q9NYF8-3; Q9NYF8; Q9NYF8-2 1 4 0.0054 0 1 1 486.02207 1941.06645 1941.06612 0.17 8e-05 931969.5 CID (Collision Induced Dissociation) MS2 0.0 30 102.8 60.0 48.8119 13570 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 89.8 92.3 78.7 110.9 107.2 110.6 118.4 112.6 99.1 108.6 Excluded by Method 3.68 1 0.00757 0.08009 False High Sequest HT (A2) Unambiguous SSEASPTAQRSK [K].sSEAsPTAQRSk.[D] N-Term(TMT6plex); S5(Phospho); K12(TMT6plex) 1 Q7Z569 Q7Z569 1 3 0.1401 0 1 1 596.30803 1786.90953 1786.91006 -0.3 -0.00018 91072.59375 CID (Collision Induced Dissociation) MS2 0.0 20 32.7 60.0 30.6042 7016 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 36.1 33.6 28.7 36.7 27.2 27.4 40.0 31.4 32.9 32.5 Excluded by Method 1.57 1 0.00757 0.07887 False High Sequest HT (A2) Unambiguous RSPSPEEK [K].rSPsPEEk.[A] N-Term(TMT6plex); S4(Phospho); K8(TMT6plex) 1 P25686 P25686 1 3 0.1768 0 1 1 489.92499 1467.76042 1467.76088 -0.32 -0.00015 179251.28125 CID (Collision Induced Dissociation) MS2 6.245537 20 17.6 60.0 32.7259 7761 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 17.3 19.4 11.9 21.3 16.4 19.9 19.5 16.3 17.9 16.4 Excluded by Method 1.98 1 0.007684 0.08143 False High Sequest HT (A2) Unambiguous EIPASPSRTK [K].eIPAsPSRTk.[I] N-Term(TMT6plex); S5(Phospho); K10(TMT6plex) 1 Q9NYZ3 Q9NYZ3 1 3 0.1979 0 1 1 541.96723 1623.88714 1623.88714 0.0 0.0 2220053.25 CID (Collision Induced Dissociation) MS2 9.653593 20 29.0 60.0 51.956 14713 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 30.1 30.0 22.2 27.0 31.9 30.4 32.6 27.7 32.1 26.3 Excluded by Method 1.92 1 0.007684 0.08131 False High Sequest HT (A2) Unambiguous LEGADDRLEADDSDDLENIDSGDLALYK [K].lEGADDRLEADDsDDLENIDSGDLALYk.[D] N-Term(TMT6plex); S13(Phospho); K28(TMT6plex) 1 Q12513 Q12513 1 3 0.4 0 1 1 1202.56565 3605.68239 3605.6784 1.11 0.00133 399016.5625 CID (Collision Induced Dissociation) MS2 27.597 60 4.8 60.0 122.8515 41195 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 4.0 10.1 1.9 3.4 10.5 3.5 11.4 3.5 Excluded by Method 2.85 1 0.008159 0.08507 False High Sequest HT (A2) Unambiguous DEESAEVEDVHDDVESDDEI [K].dEESAEVEDVHDDVEsDDEI.[-] N-Term(TMT6plex); S16(Phospho) 1 P53904 P53904 0 3 0.551 0 1 1 862.34278 2585.01378 2585.01371 0.03 2e-05 1222155.0 CID (Collision Induced Dissociation) MS2 0.0 20 53.6 60.0 84.2083 26772 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 16.8 41.9 80.0 17.3 40.7 101.4 17.7 53.0 115.2 51.9 Excluded by Method 2.94 1 0.008284 0.08616 False High Sequest HT (A2) Unambiguous GDDDEESDEEAVKK [K].gDDDEEsDEEAVkk.[T] N-Term(TMT6plex); S7(Phospho); K13(TMT6plex); K14(TMT6plex) 1 O43395 O43395 1 3 0.3508 0 1 1 778.37188 2333.10109 2333.10006 0.44 0.00034 1379927.625 CID (Collision Induced Dissociation) MS2 6.249825 10 21.9 60.0 56.1163 16255 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 26.7 24.0 16.0 19.5 17.8 22.3 23.7 22.8 23.7 22.5 Excluded by Method 2.48 1 0.008411 0.08635 False High Sequest HT (A2) Unambiguous ELGDAYVSSDEEF [K].eLGDAYVssDEEF.[-] N-Term(TMT6plex); S8(Phospho); S9(Phospho) 1 P16521 P16521 0 2 0.1268 0 1 1 925.35337 1849.69947 1849.70195 -1.34 -0.00124 349067.75 CID (Collision Induced Dissociation) MS2 24.42516 30 48.4 60.0 140.3198 47870 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 15.3 40.0 71.5 16.7 38.9 86.6 16.1 45.6 99.0 54.8 Excluded by Method 2.05 1 0.00903 0.08949 False High Sequest HT (A2) Unambiguous YNDVIEDESDDDNMSTDEAK [K].yNDVIEDESDDDNmSTDEAk.[N] N-Term(TMT6plex); Y1(Phospho); M14(Oxidation); K20(TMT6plex) 1 P48415 P48415 0 3 0.0508 0 1 1 953.73069 2859.17753 2859.18035 -0.99 -0.00094 505367.28125 CID (Collision Induced Dissociation) MS2 0.0 20 26.8 60.0 77.7333 24312 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 10.5 24.6 35.1 10.4 21.3 47.2 14.7 25.8 48.3 29.8 Excluded by Method 1.77 1 0.00903 0.08909 False High Sequest HT (A2) Unambiguous NAEEESESEAEEGD [K].nAEEEsEsEAEEGD.[-] N-Term(TMT6plex); S6(Phospho); S8(Phospho) 4 Q7L1Q6 Q7L1Q6-2; Q7L1Q6-4; Q7L1Q6-3; Q7L1Q6 0 2 0.8932 0 1 1 957.32446 1913.64165 1913.6412 0.23 0.00022 128807.6484375 CID (Collision Induced Dissociation) MS2 0.0 50 4.5 60.0 42.7372 11354 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 2.9 4.0 3.4 7.0 2.3 6.8 3.7 3.0 8.6 3.6 Excluded by Method 1.03 1 0.009137 0.09099 False High Sequest HT (A2) Unambiguous KTSTSPPPEK [K].kTsTsPPPEk.[S] N-Term(TMT6plex); K1(TMT6plex); S3(Phospho); S5(Phospho); K10(TMT6plex) 2 Q9H1E3 Q9H1E3; Q9H1E3-2 1 3 0.0108 0 1 1 640.33465 1918.9894 1918.98952 -0.06 -4e-05 941208.75 CID (Collision Induced Dissociation) MS2 10.03879 20 94.0 60.0 51.0717 14401 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 96.0 94.2 73.6 91.7 89.1 99.7 109.6 99.4 91.9 94.8 Excluded by Method 2.79 1 0.009137 0.09094 False High Sequest HT (A2) Unambiguous LETDESPIQTK [K].lETDEsPIQTk.[S] N-Term(TMT6plex); S6(Phospho); K11(TMT6plex) 1 P23585 P23585 0 3 0.172 0 1 1 600.31199 1798.92141 1798.92398 -1.43 -0.00086 3637087.5 CID (Collision Induced Dissociation) MS2 42.65182 20 54.2 60.0 76.0438 23759 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_3.raw F8.1 37.0 45.4 62.5 38.3 49.0 72.1 49.1 56.0 81.3 51.6 Excluded by Method 3.14 1 0.009245 0.09202 False High Sequest HT (A2) Unambiguous AEDGATPSPSNETPK [K].aEDGAtPSPSNEtPk.[K] N-Term(TMT6plex); T6(Phospho); T13(Phospho); K15(TMT6plex) 1 P29966 P29966 0 3 0.1512 0 1 1 706.98901 2118.95249 2118.93978 6.0 0.00424 163918.046875 CID (Collision Induced Dissociation) MS2 59.59319 40 104.2 60.0 52.3461 14826 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 106.9 96.6 97.2 105.9 97.3 97.3 132.3 107.1 108.8 92.5 Excluded by Method 2.58 1 0.009245 0.09185 False High Sequest HT (A2) Unambiguous ETERASPIK [K].eTERAsPIk.[M] N-Term(TMT6plex); S6(Phospho); K9(TMT6plex) 3 P27816 P27816; P27816-6; P27816-2 1 3 0.3301 0 1 1 523.61997 1568.84534 1568.84494 0.25 0.00013 33328248.0 CID (Collision Induced Dissociation) MS2 4.908831 40 355.7 7.49 43.6027 11668 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 368.4 351.9 288.1 377.4 340.4 352.6 416.1 363.0 348.6 350.9 Excluded by Method 2.06 1 0.009245 0.09272 False High Sequest HT (A2) Unambiguous DSTNEFVGSPSLTSPQYIPSPLSSTKPPGSVNSAAK [K].dSTNEFVGSPSLTsPQYIPsPLSSTkPPGSVNSAAk.[Y] N-Term(TMT6plex); S14(Phospho); S20(Phospho); K26(TMT6plex); K36(TMT6plex) 1 P53114 P53114 0 4 0.0047 0 1 1 1124.56197 4495.22605 4495.22898 -0.65 -0.00073 269380.78125 CID (Collision Induced Dissociation) MS2 0.0 40 5.2 60.0 119.9231 40126 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_2.raw F8.2 4.0 9.8 3.9 8.6 2.9 6.5 9.5 6.7 Excluded by Method 2.14 1 0.009245 0.09259 False High Sequest HT (A2) Unambiguous DISASSPNLGGILK [K].dIsASSPNLGGILk.[A] N-Term(TMT6plex); S3(Phospho); K14(TMT6plex) 1 Q08548 Q08548 0 3 0.0383 0 1 1 637.35163 1910.04033 1910.04002 0.17 0.00011 330798.40625 CID (Collision Induced Dissociation) MS2 0.0 60 66.4 60.0 116.7302 38898 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 20.8 55.3 93.5 18.9 59.3 124.6 23.3 73.3 132.5 62.4 Excluded by Method 2.35 1 0.009245 0.09176 False High Sequest HT (A2) Unambiguous LSGFSFK [K].lSGFsFk.[K] N-Term(TMT6plex); S5(Phospho); K7(TMT6plex) 1 P29966 P29966 0 2 0.369 0 1 1 662.35946 1323.71164 1323.71141 0.17 0.00011 5502106.0 CID (Collision Induced Dissociation) MS2 4.838619 10 103.9 35.43 113.6649 37783 20171106_LUMOS1_nLC13_AH_TechBench2_TMTMS3L_OTMC_1.raw F8.3 102.8 104.0 89.8 105.1 95.6 106.2 121.4 116.6 96.8 100.8 Excluded by Method 2.52 1 0.009615 0.09512